[Histonet] RE: Handling Muscle, Nerves and Renal biopsy specimens
Ade: I would agree with Nancy on muscle and nerve biopsies but not for renal biopsies. We have a tech on call to handle 'emergent' renal/liver biopsies on the weekends and holidays. These do happen rather frequently outside of regular business hours at my institution. How you choose to handle these will depend on the frequency and urgency of the situation at your institution. You can send me a separate email if you want more details. Joe joseph-galbra...@uiowa.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Monday, November 21, 2011 8:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Handling Muscle, Nerves and Renal biopsy specimens These cases are only scheduled during regular hours M-F. Nancy - Message: 1 Date: Sun, 20 Nov 2011 00:34:44 -0500 From: ADESUPO ADESUYI adesupo2...@hotmail.com Subject: [Histonet] Handling Muscle, Nerves and Renal biopsy specimens To: histonet@lists.utsouthwestern.edu Message-ID: snt136-w60bf15eec992cbd6e54fa3b6...@phx.gbl Content-Type: text/plain; charset=iso-8859-1 Hi, Please I am wondering if you guys could help me by telling me how you handle specimens like Muscle, Nerve and Renal biopsy after the working hours like may be at night or weekend. Thank you all for your usual cooperation. Ade. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cat Scratch fever
No one under 30 would have a clue what that joke means. Lol Joe -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Wednesday, October 12, 2011 8:38 AM To: Andrew Byrnes; Mauger, Joanne Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cat Scratch fever Too true! Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Andrew Byrnes Sent: Wednesday, October 12, 2011 8:31 AM To: Mauger, Joanne Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cat Scratch fever Good song! Andrew Byrnes AccelPath.com On Oct 12, 2011, at 7:35 AM, Mauger, Joanne mau...@email.chop.edu wrote: Newcomer Supply sells cat scratch control slides. Jo -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jessica Snay Sent: Wednesday, October 12, 2011 1:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cat Scratch fever Does anyone know where I can get a paraffin control block for cat scratch fever? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] frozen breast tissue
Hello everyone: I have a research project that wants to collect and study breast tissue both tumor and normal for long range studies (not clearly defined) that would include DNA/RNA/mRNA/protein expression along with many other possibilities. I would like your expertise (particularly from the researchers) as to what methods of preservation and storage you would recommend. Currently we have been snap freezing foil wrapped fresh tissue at -80 using a SnapFrost unit and then storing at -130 and also doing the same with a small OCT embedding piece of tissue as well. I'm concerned about the ability to cut frozen sections on the OCT specimen. Particularly, I'd be interested in opinions regarding any options for embedding and freezing fresh tissue that might enhance the ability to cut frozen sections rather than having to resort to FFPE. What are your thoughts and experiences? Thanks. Joe joseph-galbra...@uiowa.edumailto:joseph-galbra...@uiowa.edu Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain?
Amber: Actually, no we do not dump the items you listed down the drain. They get recycled appropriately. Joe -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Friday, September 30, 2011 9:25 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain? What about Alcohol formalin from the processor? The diluted alcohol (100, 95, and 75%) and the alcohol from the cleaning cycle? Do you not pour that down the drain with water? And, what about the HE stainer...do you not pour the bluing, hematoxylin, eosin and acid alcohol down the drain? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yaskovich, Ruth A (NIH/NIDCR) [E] Sent: Friday, September 30, 2011 9:06 AM To: Steve McClain; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain? Steve, I totally agree. NOTHING but water should go down the drain. Ruth N.I.H. -Original Message- From: Steve McClain [mailto:ste...@mcclainlab.com] Sent: Friday, September 30, 2011 9:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain? The argument against using toxic, yet recyclable xylene in favor of a more expensive, less efficacious xylene-substitute like Slide Bright in larger labs is not compelling. Aside from low volatility of Slide-Bright, what is gained in using a more expensive substitute whose toxicities are not well-known? The chemical composition differs, yet most MSDS warning are the same. The advantage for low volatility can be an advantage for small hole in the wall lab settings, like an office Mohs lab or frozen section lab in the OR suite where ventilation may be an issue. Unlike xylene where 75% recycling yield is norm , Slide-Bright can be recycled- how well, I don't know. I've requested specifics from B/R for a protocol and will forward that info later. However, if Slide-Bright is disposed like xylene it carries the same disposal cost. The company indicates Slide-Bright is a flammable aliphatic hydrocarbon which laden with paraffin may be disposed of down the drain with copious amounts of water, yet it is the lab directors job to ensure all local state and federal guidelines are followed. Aren't you defeating part of your purpose in working toward a safer lab and greener environment by dumping aliphatic hydrocarbons into your ground water? Steve Steve A. McClain, MD McClain Labs, LLC 45 Manor Road, Smithtown, NY 11787 631 361 4000 Slide-Bright MSDS follows: Revision Date: 6/1/2006 MATERIAL SAFETY DATA SHEET Conforms to 93/112/EC and ISO 11014-1 1. CHEMICAL PRODUCT AND COMPANY IDENTIFICATION PRODUCT NAME:OptiClear E PRODUCT NUMBER: OE-104 CHEMICAL NAMES/ DESCRIPTION: Aliphatic Hydrocarbon MANUFACTURER: National Diagnostics, Inc. 305 Patton Drive Atlanta, GA 30336 TELEPHONE NUMBER: (800) 526-3867 (404) 699-2121 EMERGENCY NUMBER: CHEMTREC (800) 424-9300 2. COMPOSITION / INFORMATION ON INGREDIENTS Component% Comp CAS #EINECS # TLV (units) Aliphatic Hydrocarbons EEC LABEL SYMBOL AND CLASSIFICATION R: 11-38 100 400 ppm Highly flammable. Irritating to skin. S: (2-) 16-23-24-62 Keep out of the reach of children. Keep away from sources of ignition - No Smoking. Do not breathe fumes. Avoid contact with the skin. If swallowed, do not induce vomiting: Seek medical advice immediately and show this container or label. 3. HAZARDS IDENTIFICATION APPEARANCE AND ODOR: Clear, colorless liquid EMERGENCY OVERVIEW - IMMEDIATE HAZARD HIGHLY FLAMMABLE. PRODUCT IS SLIGHTLY IRRITATING TO EYES (NO INJURY). HIGH VAPOR MAY CAUSE RESPIRATORY TRACT IRRITATION, HEADACHE, DIZZINESS, ANESTHESIA, DROWSINESS, UNCONSCIOUSNESS, OR DEATH. INGESTION: MINIMAL TOXICITY. ASPIRATION MAY LEAD TO PULMONARY INJURY AND DEATH. EMERGENCY OVERVIEW - CHRONIC HAZARD W ARNING NO CHRONIC HAZARDS SUSPECTED. POTENTIAL HEALTH EFFECTS INHALATION High vapor/aerosol concentrations (greater than approximately 1000 ppm) are irritating to the eyes and the respiratory tract, may cause headaches, dizziness, anesthesia, drowsiness, unconsciousness, and other central nervous system effects, including death. INGESTION Minimal toxicity by ingestion, though small amounts of this product aspirated into the respiratory system durin ingestion or vomiting may cause mild to severe pulmonary injury, possibly progressing to death. SKIN Low order
RE: [Histonet] Fungus Control Tissue
Sheila: Have you tried your local or state animal disease lab or the National Animal Disease Lab in Ames, IA. If you can work with animal instead of human tissue these labs may be able to help. Do you have a large academic or tertiary care medical center nearby which has a block reclamation program (collects anonymized tissue blocks for research purposes that are being discarded by labs after the required holding period). Sometimes these programs can find blocks with unusual or infectious processes as they collect from a broad range of sources. (PS: We have such a program here.) Joe -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Thursday, September 29, 2011 12:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fungus Control Tissue Hi all. Does anyone have a resource for control blocks? We are in need of fungus controls and are trying to save the cost of buying slides. Thanks all. Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin
Histoland: I have to agree also. Programs that are graduating students with none of the skills listed in Matt's message are not doing their students or the profession any favors. Programs certified to produce graduates should be required to place these students in rotations that give them practical experience and manual skills. As a University based hospital we collaborate with area programs to provide their students with a practical rotation. Believe me they must get up to speed quickly under our tutelage and leave having learned the skills or they do not pass our rotation. If programs are just training students to pass the ASCP written exam without any practical experience either on site at the program or in collaboration with real labs then that is indeed a sad state of affairs and one that we as professionals should address via NSH and ASCP. The practical may not have been the answer for everyone but we should not allow students to graduate without basic practical skills. Thanks. Joe Galbraith Univ of Iowa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Tuesday, August 30, 2011 7:32 PM To: Weems, Joyce; Matthew Lunetta; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin I second that Joyce. sp From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce [jwe...@sjha.org] Sent: Tuesday, August 30, 2011 6:17 PM To: Matthew Lunetta; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin I don't understand how a student of any program would have not a portion of their program dedicated to these skills. We partner with Darton College and their students to do a certain number of hours for their Clinicals. They know how to do those things, are trained by the clinical coordinator for the program, and are graded on their work. Are they prepared to go into a lab and work like they've done OJT for 1-2 years? Not at all, but they need to be hired with the understanding that they will need time and patience to develop their speed and their skill. My 2 cents... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta Sent: Tuesday, August 30, 2011 13:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin Hey all, I found Peggy's comments on why the practical was discontinued to be very interesting. Of late I have had some experience with a new HT that graduated from a program and passed the current HT exam. So, as they say in Great Britain, to stick a pin in the ASCP reasons. This new fresh and shiny HT has all the book knowledge we needed them to have. What they did not have was any technical skills. 1) never used a microscope or centrifuge. 2) no special staining experience 3) no embedding experience 4) no cutting experience When they cut or embed they are no were near the speed, accuracy or quality that is needed in our industry. While they can answer any question you ask them they just do not have the technical skills one would expect from a new graduate. I have learned several lessons from this experience. 1) I am so very glad I was one of the last HT's to have taken the practical 2) Any new HT's will be taking a practical if I am involved in the selection process. 3) I will question they quality of any new HT from this particular program While I am sure that there are many new HT's that do have the skills needed, this one experience has caused me to be more cautious. Respectfully, Matt Lunetta BS, HT (ASCP) Message: 2 Date: Tue, 30 Aug 2011 18:09:46 +0200 From: Gudrun Lang Subject: AW: [Histonet] Re: peggy wenk comments on HT/HTL practical To: 'Bob Richmond' Cc: histonet@lists.utsouthwestern.edu Message-ID: 8b7976b131854abc8db236fab5026...@dielangs.at Content-Type: text/plain; charset=iso-8859-1 Dear Dr. Richmond Here in Austria we have a job open for a pathologist with 5 years experience. ;) Please, think it over to come. Lovely mountains, lovely techs... It sounds, like you are from that sort of pathologist techs dream of. Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Bob Richmond Gesendet: Dienstag, 30. August 2011 04:43 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: peggy wenk comments on HT/HTL practical I really appreciate Peggy Wenk's analysis of the practical examination and
FW: [Histonet] Re:peggy wenk comments on HT/HTL practical - Tostick a Pin
-Original Message- From: Galbraith, Joe Sent: Wednesday, August 31, 2011 10:13 AM To: 'Heath, Nancy L.'; 'histonet-boun...@lists.utsouthwestern.edu' Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - Tostick a Pin Nancy: I would encourage our regional and national leaders to bring this issue to the board of governors at the NSH and have the board direct the NSH executives to address ASCP regarding setting standards for the programs that with to provide 'graduates' eligible to take the exam. Perhaps the standards should include a practical rotation in some form. Perhaps a requirement for sitting for the exam should be successful completion of a practical rotation. These are just thoughts. Others may have better ideas. Joe -Original Message- From: Heath, Nancy L. [mailto:nhe...@lifespan.org] Sent: Wednesday, August 31, 2011 9:27 AM To: Galbraith, Joe Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - Tostick a Pin Totally agree with you Joe! How would we as professionals in the field organize our concerns to NSH and/or ASCP? I would think we would need a large group of histotechs to be heard. Nancy Heath, HT(ASCP) Rhode Island Hospital -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Galbraith, Joe Sent: Wednesday, August 31, 2011 9:36 AM To: Shirley A. Powell; Weems, Joyce; Matthew Lunetta; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - Tostick a Pin Histoland: I have to agree also. Programs that are graduating students with none of the skills listed in Matt's message are not doing their students or the profession any favors. Programs certified to produce graduates should be required to place these students in rotations that give them practical experience and manual skills. As a University based hospital we collaborate with area programs to provide their students with a practical rotation. Believe me they must get up to speed quickly under our tutelage and leave having learned the skills or they do not pass our rotation. If programs are just training students to pass the ASCP written exam without any practical experience either on site at the program or in collaboration with real labs then that is indeed a sad state of affairs and one that we as professionals should address via NSH and ASCP. The practical may not have been the answer for everyone but we should not allow students to graduate without basic practical skills. Thanks. Joe Galbraith Univ of Iowa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Tuesday, August 30, 2011 7:32 PM To: Weems, Joyce; Matthew Lunetta; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin I second that Joyce. sp From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce [jwe...@sjha.org] Sent: Tuesday, August 30, 2011 6:17 PM To: Matthew Lunetta; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin I don't understand how a student of any program would have not a portion of their program dedicated to these skills. We partner with Darton College and their students to do a certain number of hours for their Clinicals. They know how to do those things, are trained by the clinical coordinator for the program, and are graded on their work. Are they prepared to go into a lab and work like they've done OJT for 1-2 years? Not at all, but they need to be hired with the understanding that they will need time and patience to develop their speed and their skill. My 2 cents... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta Sent: Tuesday, August 30, 2011 13:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin Hey all, I found Peggy's comments on why the practical was discontinued to be very interesting. Of late I have had some experience with a new HT that graduated from a program and passed the current HT exam. So, as they say in Great Britain, to stick a pin in the ASCP reasons. This new fresh and shiny HT has all the book knowledge we needed them to have. What they did not have was any technical skills. 1) never used a microscope or centrifuge. 2) no special staining experience 3) no embedding experience 4) no cutting experience When they cut or embed
RE: [Histonet] RE: Embedding process improvement and competencyassessment
How disgusting to hear about cheating. I recall that someone was supposed to sign off as a witness that the applicant had done the work themselves. I spent months acquiring tissue, processing, embedding, cutting and staining a set of blocks and slides and was rewarded with a high score for the effort. It was something I could be proud of. As I recall we had to submit 25 or so slides back then only some of which were graded and the grading was really strict (but did vary with the grader). Joe Galbraith HTL (and also MT by the way) University of Iowa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra Sent: Thursday, August 25, 2011 12:14 PM To: 'Jennifer MacDonald'; Heath, Nancy L. Cc: Histonet Listserv (E-mail); histonet-boun...@lists.utsouthwestern.edu; D'Attilio, Shelley Subject: RE: [Histonet] RE: Embedding process improvement and competencyassessment I heard of a lot of cheating as well. People paid others to do the blocks and staining. How good does it do? In the end, these people are cheating themselves. Very sad! Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Thursday, August 25, 2011 7:58 AM To: Heath, Nancy L. Cc: Histonet Listserv (E-mail); histonet-boun...@lists.utsouthwestern.edu; D'Attilio, Shelley Subject: RE: [Histonet] RE: Embedding process improvement and competencyassessment I fail to see the correlation of a non HT person supervising the Histology lab and the lack of a practical exam for HT/HTL staff. One of the issues that Shelley brought up was the staff lost or did not develop their embedding skills. Submission of a practical exam is not proof of highly developed embedding skills. For the HT exam there were 8 blocks that were submitted (9 slides). I know of cases where the blocks were not even embedded or cut by the applicant. Heath, Nancy L. nhe...@lifespan.org Sent by: histonet-boun...@lists.utsouthwestern.edu 08/25/2011 07:11 AM To D'Attilio, Shelley sdatt...@stormontvail.org, Podawiltz, Thomas tpodawi...@lrgh.org, Histonet Listserv (E-mail) histonet@lists.utsouthwestern.edu cc Subject RE: [Histonet] RE: Embedding process improvement and competencyassessment This is exactly why the powers that be should have NEVER gotten rid of the practical portion of the HT/HTL board certification! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of D'Attilio, Shelley Sent: Thursday, August 25, 2011 9:45 AM To: Podawiltz, Thomas; Histonet Listserv (E-mail) Subject: [Histonet] RE: Embedding process improvement and competencyassessment Hi Tom, Thank you for your kind words. I am off the bench almost completely. I can work in the gross room in a pinch and my counting skills are excellent, so I can always file slides and block if an emergency arises:) I occasionally cover a bench in Chemistry as well, but my staff is all pretty glad that I mostly stay in my office. Thanks so much for the embedding information. The main problem we are tackling at the moment is tissue orientation. I have written a pretty detailed embedding procedure that is being reviewed by the new histology supervisor. Our plan is to refresh the training of everyone on staff in conjunction with this procedure, then add specific embedding competencies to our checklist. I will make sure that the procedure incorporates the first 6 elements that you listed below. Currently we have a QA sheet that is given to the pathologist with each batch of slides. Pathologists provide us with feedback on the slide quality by filling out the form. Slides with sub-standard quality--whether in orientation, cutting, staining, whatever--our reviewed by every histotech in the lab with an aim to education and improvement of performance. We have a form called the Slide Quality Review Form that details the quality issue. Techs are directed to review the slides and comment. Difficult cases or those where people disagree are discussed in our department meetings. One of our difficulties over the years has been how the work was divided between the histotechs. One histotech loved to embed and was very good at it, so he did most of the embedding. He eventually moved to an overnight shift, which resulted in him embedding even more than he was. Consequently, other staff people either lost their skills or never fully developed them. It was introduction of rapid processing that really brought this issue to the forefront, since different people were embedding at different times of the day. Unfortunately, I let my NSH membership lapse this year for budgetary reasons. I
RE: [Histonet] RE: New CAP question
All: It is my understanding that you should retain validation records for any testing that is still being done regardless of how long ago that validation may have occurred. In general, you need to be able to show how you traced and compared the current test to some previously validated test and that requirement does not have an expiration date to my knowledge. If the switch occurred so long ago that there was no validation requirement at the time of the switch then some inspectors may let you off the hook but I suspect that the intent here is that you would still need to be able to document that you have validated the test somehow, perhaps by redoing it today. FDA approved prognostic markers are often scrutinized more carefully than other testing. Documentation for testing that has been discontinued can eventually be discarded after a lengthy waiting period. There is indeed variation between interpretations depending on your inspector with some being very rigid and others much less so. Joe Galbraith University of Iowa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, August 25, 2011 11:46 AM To: Vickroy, Jim; Martha Ward; Carol Bryant; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New CAP question I think it may depend on the inspector. We had something similar happen in Cytology during inspection. They had no validation records for their Thin Prep processing, which they had been doing for years. They were required to validate and provide documentation to CAP. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Thursday, August 25, 2011 9:31 AM To: Martha Ward; Carol Bryant; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New CAP question I hope you're correct. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 -Original Message- From: Martha Ward [mailto:mw...@wakehealth.edu] Sent: Thursday, August 25, 2011 11:30 AM To: Carol Bryant; Vickroy, Jim; histonet@lists.utsouthwestern.edu Subject: RE: New CAP question We too have been performing ER and PR for at least 15 years, participate in CAP proficiency testing and, when we switched staining platforms a few years ago, validated the new antibody we switched to. I have interpreted the standard as necessary if you are introducing ER/PR in your lab. In my opinion you would not have to go back and revalidate something you did years ago just to have something to show at inspection time. We had our CAP inspection this summer and a similar question pertains to the HER2 assay, which we have also been doing for many years, and that is what I told our inspector, which seemed to satisfy them. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, August 25, 2011 12:10 PM To: 'Vickroy, Jim'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New CAP question Please respond to all. I would like the information also. Thank you, Carol -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Thursday, August 25, 2011 12:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New CAP question One of the new CAP questions is ANP.22976 ER/PgR validation. If the laboratory performs immunohistochemistry for estrogen receptor and/or progesterone receptor as a prognostic/predictive marker on breast carcinoma, the laboratory has documented appropriate validation for the assays. In the note it says should include a minimum of 40 cases and validation should be performed by comparing the laboratory's results with another assay that has been appropriately validated. We have been doing ER/PR's for over ten years. Originally we compared our ER/PR testing with the old immunology method that used frozen breast tissue. We also compared our ER/PR results with another hospital. Problem is that this has been over ten years and we do not keep quality control records that long. Am I missing something? I know we use the FDA approved protocol from Ventana on our Ventana Benchmark XT. Should we do another validation study using Ventana or another hospital that is using the FDA approved method? Anybody understand what CAP is wanting and how to accomplish this? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including
RE: [Histonet] Formalin down the drain??
Indeed not only county to county but your local municipality can have specific regulations related to hazardous waste discard in the sewer since it is their waste treatment plant that takes the hit so to speak. Most small municipalities will adopt state or county regulations (whichever is stricter) but they can always write even stricter rules if they wish and that may well be the case in some larger metro areas. Good luck. Joe University of Iowa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra Sent: Monday, July 25, 2011 3:56 PM To: 'O'Donnell, Bill'; mtitf...@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Formalin down the drain?? I totally agree, under any circumstances, whatever the State law defines, we should not dump formalin, xylene or alcohols down the drain. And even though California has very strict rules, it even seems to differ from county to county. In one of my past jobs we were allowed to dump the formalin down the drain (Orange County of all places), whereas in San Diego, that was absolutely prohibited. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Monday, July 25, 2011 1:20 PM To: mtitf...@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Formalin down the drain?? One should not automtically assume that laws are broken here. (Rant begins here) First of all, it is the States that set the limits of what can and cannot be dumped. All States must meet Federal standards,but States are free to determine how they do that. (It's one of the benefits of the American Revolution) Some states are more heavily regulated than others. California and Colorado come to mind immediately. Different organizations, locations and circumstances may allow for disposal of products that may be diluted to such a degree as to be negligable in the waste stream. Our institution generates 65,000 gallons of waste water daily, which allows us to make the dilution limits of anything that our histo lab could produce in a day. No laws are broken if I should pour xylene, formalin, alcohols or other common compounds that we might generate on even our busiest days into the waste stream. HOWEVER, while we may be allowed to do so by state and local regulations, we have decided it is not prudent to do so and so we collect, ship, neutralize or recycle most all that the histo lab generates. We do this at the lab level, with lab funding. It is the responsible thing to do, and we are morally and ethically bound to do so, but we are not outside the law if we do not. If your local municipal waste systems people give you the green light on dumping formalin down the drain. you are not breaking the law, federal or otherwise, in doing so. It is true that if you wish to affect things globally, one has to be responsible locally. Here is what my rant comes down to Make certain that you are meeting local standards for your chemical disposal or you may well be breaking the law. And a big thank you (from myself, my children, grandchildren and great-grand children and that lady who sells me the slurpee at the local convenience store) for anything anyone is doing above and beyond that. :)Rant is over... Have a nice day :) You cannot Like this rant on Facebook or follow this rant on Twitter. Bill -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mtitf...@aol.com Sent: Monday, July 25, 2011 12:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin down the drain?? I was a little distressed to read the message from Amy in Camp Hill, Pennsylvania declaring she dumps everything (and I mean everything) from her histology lab down the drain. There are a bunch of Federal Laws governing handling and disposal of chemicals used in the histology laboratory and she appears to be breaking several. The wastewater law limits how much formalin you can discard down the sink (and you cannot dilute as you go). The same law forbids disposal of organic solvents like xylene, or solutions containing organic solvents. Local laws in Pennsylvania may be more strict. I recommend to Amy that she purchases a book like, Hazardous materials in the histopathology laboratory by Janet Richard Dapson and read the whole thing cover to cover! Michael Titford Pathology USA Mobile AL USA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list
RE: [Histonet] batching controls
We run an appropriate positive control on every patient test slide to test that the antibody and all components used on that slide worked. This may be overkill but it helps us ensure that each slide worked properly not just the run. Joe joseph-galbra...@uiowa.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, January 11, 2011 11:44 AM To: Laurie Colbert; Taylor, Jean; ih...@googlegroups.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] batching controls How does this work if you need to send the case out for a consult and the control is on another patient's slide? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Laurie Colbert Sent: Tuesday, January 11, 2011 12:39 PM To: Taylor, Jean; ih...@googlegroups.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] batching controls We batch our controls. We still try to put the control tissue on a patient slide, and then we reference that case that has the control on the other cases that don't have a control. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean Sent: Tuesday, January 11, 2011 9:22 AM To: 'ih...@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] batching controls Hi Everyone, I'd like to know how many labs batch their controls when the same antibody is ordered on multiple cases. Do you run the control on a separate slide, or with one of the cases ordered? Thank you! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [IHCRG] RE: [Histonet] batching controls
Hadi: Just for clarification, the control is on the same slide as the patient so it is subjected to the same conditions as the patient (post sectioning). The control block from which the control is cut is QA'ed against other known positive blocks prior to use as a control. Thanks. Joe -Original Message- From: ancillaryp...@mac.com [mailto:ancillaryp...@mac.com] Sent: Tuesday, January 11, 2011 12:09 PM To: Galbraith, Joe Cc: Rathborne, Toni; Laurie Colbert; Taylor, Jean; ih...@googlegroups.com; histonet@lists.utsouthwestern.edu Subject: Re: [IHCRG] RE: [Histonet] batching controls This is the most cautious approach, and for antibodies where there is no internal controls that would be your only way to verify that the studies worked. But it's also associated with some unnecessary rejection of good studies because controls aren't treated the same way as the patient slides. Good approach and we do it on our technical-only cases where the slides are returned to the outside pathologists for interpretation. Hadi On Jan 11, 2011, at 1:03 PM, Galbraith, Joe wrote: We run an appropriate positive control on every patient test slide to test that the antibody and all components used on that slide worked. This may be overkill but it helps us ensure that each slide worked properly not just the run. Joe joseph-galbra...@uiowa.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, January 11, 2011 11:44 AM To: Laurie Colbert; Taylor, Jean; ih...@googlegroups.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] batching controls How does this work if you need to send the case out for a consult and the control is on another patient's slide? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Laurie Colbert Sent: Tuesday, January 11, 2011 12:39 PM To: Taylor, Jean; ih...@googlegroups.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] batching controls We batch our controls. We still try to put the control tissue on a patient slide, and then we reference that case that has the control on the other cases that don't have a control. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean Sent: Tuesday, January 11, 2011 9:22 AM To: 'ih...@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] batching controls Hi Everyone, I'd like to know how many labs batch their controls when the same antibody is ordered on multiple cases. Do you run the control on a separate slide, or with one of the cases ordered? Thank you! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. -- You received this message because you are subscribed to the Google Groups ihcrg group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. To post to this group, send email to ih...@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscr...@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en To contact the National Society for Histotechnology, email: hi...@nsh.org or call 443.535.4060. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [IHCRG] RE: [Histonet] batching controls
Hadi: I concur with your comments completely. We attempt to make the conditions the same to the extent possible but it is never exact. Facing the block should cut away surface oxidation in most cases. We avoid precutting large numbers of controls (particularly for antibodies known to be particularly sensitive to tissue oxidation where we will cut the control on the same day, often at the same time, as the patient, taking care to avoid cross contamination). There are so many variables to consider. Testing for oxidative decay should probably be standard operating procedure for control block evaluation especially for new antibodies. Internal controls are always the best from this standpoint. Best wishes. Joe -Original Message- From: Hadi Yaziji [mailto:ancillaryp...@mac.com] Sent: Tuesday, January 11, 2011 1:07 PM To: Galbraith, Joe Cc: Rathborne, Toni; Laurie Colbert; Taylor, Jean; ih...@googlegroups.com; histonet@lists.utsouthwestern.edu Subject: Re: [IHCRG] RE: [Histonet] batching controls As you guessed correctly, I wasn't referring to analytical conditions. Control tissues are often fixed at longer times than patient tissues. Secondly, the surface in the control tissue blocks is often oxidized. Also, when control slides are cut, some labs cut dozens of controls from the same block and store the slides. All of these conditions make the control slides lose much of the sensitivity depending on the different parameters mentioned above. That is why in our consensus articles published in AIMM in 2007 and 2008, we insisted to put benign breast tissue in the same tumor section to make sure the positive controls are treated identically to the patient tissue. This is the only way to ensure the controls are treated the same way as patient tissue. What you correctly referred to is only a small part of the whole picture. Hadi On Jan 11, 2011, at 1:51 PM, Galbraith, Joe wrote: Hadi: Just for clarification, the control is on the same slide as the patient so it is subjected to the same conditions as the patient (post sectioning). The control block from which the control is cut is QA'ed against other known positive blocks prior to use as a control. Thanks. Joe -Original Message- From: ancillaryp...@mac.com [mailto:ancillaryp...@mac.com] Sent: Tuesday, January 11, 2011 12:09 PM To: Galbraith, Joe Cc: Rathborne, Toni; Laurie Colbert; Taylor, Jean; ih...@googlegroups.com; histonet@lists.utsouthwestern.edu Subject: Re: [IHCRG] RE: [Histonet] batching controls This is the most cautious approach, and for antibodies where there is no internal controls that would be your only way to verify that the studies worked. But it's also associated with some unnecessary rejection of good studies because controls aren't treated the same way as the patient slides. Good approach and we do it on our technical-only cases where the slides are returned to the outside pathologists for interpretation. Hadi On Jan 11, 2011, at 1:03 PM, Galbraith, Joe wrote: We run an appropriate positive control on every patient test slide to test that the antibody and all components used on that slide worked. This may be overkill but it helps us ensure that each slide worked properly not just the run. Joe joseph-galbra...@uiowa.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, January 11, 2011 11:44 AM To: Laurie Colbert; Taylor, Jean; ih...@googlegroups.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] batching controls How does this work if you need to send the case out for a consult and the control is on another patient's slide? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Laurie Colbert Sent: Tuesday, January 11, 2011 12:39 PM To: Taylor, Jean; ih...@googlegroups.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] batching controls We batch our controls. We still try to put the control tissue on a patient slide, and then we reference that case that has the control on the other cases that don't have a control. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean Sent: Tuesday, January 11, 2011 9:22 AM To: 'ih...@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] batching controls Hi Everyone, I'd like to know how many labs batch their controls when the same antibody is ordered on multiple cases. Do you run the control on a separate slide, or with one of the cases ordered? Thank you! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI ___ Histonet
RE: [Histonet] human macrophages in human melanoma xenografts
Naira: Here is a link to a site listing macrophage markers. F4/80 is a commonly mentioned marker for macrophages. I presume you mean IHC rather than ICH. Enjoy. http://www.antibodybeyond.com/reviews/cell-markers/macrophage-marker.htm Joe joseph-galbra...@uiowa.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Friday, October 23, 2009 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] human macrophages in human melanoma xenografts Hi Histonetters, I would like to be able to look at human macrophages in human melanoma xenografts raised in mouse. Could you please suggest me a best Ab for ICH and protocol? Thanks in advance, Naira ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] human macrophages in human melanoma xenografts
Liz: Thanks for clarifying. Your comments are spot on. That's what I get for responding too quickly and not thinking things through. I would agree with Naira that red detection is useful in the melanoma context. Joe 380 MRC 4-4737 (voice) 3-3482 (fax) pager 131-1170 joseph-galbra...@uiowa.edu -Original Message- From: Liz Chlipala [mailto:l...@premierlab.com] Sent: Friday, October 23, 2009 11:00 AM To: Margaryan, Naira; Galbraith, Joe; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts That will work but I'm not aware of the non-mouse anti-human macrophage marker, you could check the web or biocompare. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: Margaryan, Naira [mailto:nmargar...@childrensmemorial.org] Sent: Friday, October 23, 2009 9:57 AM To: Liz Chlipala; Galbraith, Joe; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts Thanks a lot both of you! For melanoma detection, I usually use HRP with AEC. Is there any non-mouse anti-human macrophage marker? Thank you much, Naira -Original Message- From: Liz Chlipala [mailto:l...@premierlab.com] Sent: Friday, October 23, 2009 10:51 AM To: Galbraith, Joe; Margaryan, Naira; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts F4/80 is a mouse macrophage marker. If she wants to detect human macrophages in a mouse background she will need to use a mouse anti-human CD68. She will need to run it with a mouse on mouse detection system and run all of the appropriate negative controls. I would also select a alkaline phosphatase detection system rather than HRP with DAB just incase the tumor has any melanin in it. I have done this before looking for human lymphocytes with LCA in a mouse background. You just need to make sure you have all of the appropriate controls. For a positive control I would use a human tonsil or something like that and you need to make sure you run the appropriate isotype negative controls. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Galbraith, Joe Sent: Friday, October 23, 2009 9:44 AM To: Margaryan, Naira; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts Naira: Here is a link to a site listing macrophage markers. F4/80 is a commonly mentioned marker for macrophages. I presume you mean IHC rather than ICH. Enjoy. http://www.antibodybeyond.com/reviews/cell-markers/macrophage-marker.htm Joe joseph-galbra...@uiowa.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Friday, October 23, 2009 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] human macrophages in human melanoma xenografts Hi Histonetters, I would like to be able to look at human macrophages in human melanoma xenografts raised in mouse. Could you please suggest me a best Ab for ICH and protocol? Thanks in advance, Naira ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE:Missing the point of Plants-in-the-lab OT
All: We had an inspector comment that plants were discouraged only because of the possibility that the plant pollens or the microbes on the plants or in the soil could end up on the water bath (and other work surfaces) and hence ultimately (directly or indirectly) in the tissue on the slides where the pathologist may have to determine if they were contaminants or integral to the tissue. Most of the time, this would be trivial but in some cases the issue could be substantive. So we were told. Sadly, we no longer have plants in the lab despite their positive impact on air quality and employee satisfaction. Joe joseph-galbra...@uiowa.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McMahon, Loralee A Sent: Thursday, October 22, 2009 2:31 PM To: Akemi Allison-Tacha; Sara''Breeden; histonet@lists.utsouthwestern.edu; LindaBlazek; Patti Loykasek Subject: RE: [Histonet] RE:Missing the point of Plants-in-the-lab OT I just want to know what CAP question states that you cannot have plants in the lab. Or is this the inspectors interpretation of a CAP question. Maybe the question regarding the lab working conditions. I could see if you have plants all over the counters crowding the space. But that is not regarding the plants themselves that would be in regard to having a crowded work environment. ?? When you give a phase 1 deficiency you have to reference the CAP question. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha [akemiat3...@yahoo.com] Sent: Thursday, October 22, 2009 3:26 PM To: Sara''Breeden; histonet@lists.utsouthwestern.edu; LindaBlazek; Patti Loykasek Subject: Re: [Histonet] RE:Missing the point of Plants-in-the-lab OT Hi All, I think all of you are missing the point of Patti's question. She stated that her lab was dinged for having plants in the lab by a CAP inspector. I had the same thing happen to me years ago. The inspector stated that plants attract insects that can contaminate a supposedly clean environment. Patti has an extremely well organized lab that only had a small phase (1) deficiency last year. I think the inspector couldn't find anything, so they had to come up with this ridiculous infraction. Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingTele: 408.402.5257 Cell: 408.335.9994 E-Mail: akemiat3...@yahoo.com --- On Thu, 10/22/09, Blazek, Linda lbla...@digestivespecialists.com wrote: From: Blazek, Linda lbla...@digestivespecialists.com Subject: [Histonet] RE: Plants-in-the-lab OT To: 'Breeden, Sara' sbree...@nmda.nmsu.edu, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Thursday, October 22, 2009, 12:15 PM I don't know Sally, but where I worked many moons ago I had a spider plant that was extremely prolific. The powers that be made me remove it from the lab for an inspection. It went to live in one of the administrator's office for several months. And died! I think it needed the fumes! Or it missed me. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Phone: (937) 293-4424 ext 7118 Email: lbla...@digestivespecialists.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, October 22, 2009 3:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Plants-in-the-lab OT I think it's the fluorescent lights that makes them thrive. The absorption of Fume Matter is a secondary, but beneficial, effect. You go, chlorophyll! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] DNA Extraction
Sharon: Here is just one of many such protocols published recently. Commercial kits are available from molecular diagnostic suppliers (we use a Qiagen kit for example - there are certainly numerous others). http://www.protocol-online.org/prot/Protocols/DNA-Extraction-from-Archiv al-Formalin-fixed--Paraffin-embedded-Tissue-Sections-3159.html Joe 380 MRC 4-4737 (voice) 3-3482 (fax) pager 131-1170 joseph-galbra...@uiowa.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shaw, Sharon Sent: Monday, September 14, 2009 9:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DNA Extraction Hello Histo land, Does anyone know how to extract DNA from formalin fixed paraffin embedded tissue? Thanks, Sharon WPI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Company email
Sheila: Often on-line vendors do not 'like' and sometimes do not accept 'free-mailer' email addresses like yahoo, g-mail, hotmail, etc. because they are frequently faked or spoofed. This is often an issue with the payment processor (the company the supplier hires to process credit cards) not the supplier directly. These processors frequently get burned by scam artists and it can sometimes cost the processor lots of money, hence their reluctance to process free email addresses. They are looking for pay for service addresses from internet access providers like Quest, Mediacom, or your company, etc. For those (and there are many of us these days) who save money by using a free-mailer, this issue can be a problem. You can complain to the supplier and they may be able to pressure the processor to some degree but it is almost certainly not directly their issue. Look to see whose secure site you are transferred to when you go to pay for your purchase on-line. That may tell you the company who is actually setting this rule. If Beckman is actually processing their payments and making the rule directly, then try using a different email address (the best ones that are set up via your own company, ie yourn...@yourcompany.com). In the absence of a 'real' email addy, I presume you have tried the telephone ordering approach. If they won't take an order over the phone without a 'real' email addy, then you are in a world of hurt. Be sure that you are using a physical address for your location and ship to address that is trusted and can be independently confirmed. That may help them be less concerned. Best of luck. Joe joseph-galbra...@uiowa.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Monday, September 14, 2009 11:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Company email I just ran into an interesting issue that I thought I'd share. I was trying to place a credit card order with Beckman Coulter but was denied due to them not liking my email address. Mind you, I was willing to pay with a credit card. I placed the order on 9/2 and have been fighting with them ever since. Ridiculous! Today they were standing by their policy of not accepting my email address as a business address so I'm taking my business elsewhere. Just thought I'd share this with those of you looking for a company to do business with. Sheila Haas Laboratory Supervisor Micro Path Laboratories ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Company email
Sometimes the best way to complain is with the feet. Joe joseph-galbra...@uiowa.edu From: Sheila Haas [mailto:micropathl...@yahoo.com] Sent: Monday, September 14, 2009 12:33 PM To: Galbraith, Joe Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Company email I've jumped through hoops already and made a dozen calls to the company (they are refusing) to no evail so I suppose hurt is accurate but not for long. There are many other companies out their to order from so I'll just move down the list. Thanks, Sheila Haas Laboratory Supervisor Micro Path Laboratories From: Galbraith, Joe joseph-galbra...@uiowa.edu To: Sheila Haas micropathl...@yahoo.com Cc: histonet@lists.utsouthwestern.edu Sent: Monday, September 14, 2009 1:05:49 PM Subject: RE: [Histonet] Company email Sheila: Often on-line vendors do not 'like' and sometimes do not accept 'free-mailer' email addresses like yahoo, g-mail, hotmail, etc. because they are frequently faked or spoofed. This is often an issue with the payment processor (the company the supplier hires to process credit cards) not the supplier directly. These processors frequently get burned by scam artists and it can sometimes cost the processor lots of money, hence their reluctance to process free email addresses. They are looking for pay for service addresses from internet access providers like Quest, Mediacom, or your company, etc. For those (and there are many of us these days) who save money by using a free-mailer, this issue can be a problem. You can complain to the supplier and they may be able to pressure the processor to some degree but it is almost certainly not directly their issue. Look to see whose secure site you are transferred to when you go to pay for your purchase on-line. That may tell you the company who is actually setting this rule. If Beckman is actually processing their payments and making the rule directly, then try using a different email address (the best ones that are set up via your own company, ie yourn...@yourcompany.com). In the absence of a 'real' email addy, I presume you have tried the telephone ordering approach. If they won't take an order over the phone without a 'real' email addy, then you are in a world of hurt. Be sure that you are using a physical address for your location and ship to address that is trusted and can be independently confirmed. That may help them be less concerned. Best of luck. Joe joseph-galbra...@uiowa.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Monday, September 14, 2009 11:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Company email I just ran into an interesting issue that I thought I'd share. I was trying to place a credit card order with Beckman Coulter but was denied due to them not liking my email address. Mind you, I was willing to pay with a credit card. I placed the order on 9/2 and have been fighting with them ever since. Ridiculous! Today they were standing by their policy of not accepting my email address as a business address so I'm taking my business elsewhere. Just thought I'd share this with those of you looking for a company to do business with. Sheila Haas Laboratory Supervisor Micro Path Laboratories ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] formalin storage
Amy: Exactly what do you mean by 'we store all our chemicals in a flammable storage cabinet'? By any chance, do you mean that you actually put everything into the same cabinet together? I would be very concerned about putting everything into one cabinet (flammable or not). Some materials should not be stored together (it's not safe and regulations require separate storage based on reactive properties). If this is the case, please consult your safety officer or use the MSDS sheet or the internet to determine proper storage. Joe Galbraith University of Iowa joseph-galbra...@uiowa.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Friday, September 11, 2009 8:44 AM To: Riesen, Rebecca; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] formalin storage We store all our chemicals in a flammable storage cabinet -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Riesen, Rebecca Sent: Friday, September 11, 2009 9:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] formalin storage We have been directed by our Safety Officer to store all formalin (37% and 10% NBF) in a flammable storage room, cabinet or container. Yes, 37% Formalin we do store in this manner, but I have never heard of this requirement for 10%NBF. I looked on line to many MSDS sheets from different vendors and found only one that stated such storage requirements for 10% NBF. During this search I found all but one company states that formalin is not flammable. I brought this to the Safety Officer. He agrees that it is not flammable but that it IS combustible. Combustible=Flash point of 100F to 200F. Of the dozen sites I visited I found the following data concerning the Flash Point of 10% NBF: from NA / 200F / 122F to 185F. The NFPA (National Fire Protection Agency) guideline of no more than 1 gallon in a flammable storage container and 1 gallon outside of a safety cabinet/container per 100 square feet is already quite limiting. Using this guideline, we have calculated acceptable volumes of the known flammables (Alcohols and Xylenes) we can store. Adding 10% NBF to the equation will have us traveling to our bulk storage area constantly. Does anyone out there store 10%NBF in flammable cans/cabinets? Riesen, Rebecca rebecca.rie...@nchmd.org NCH Healthcare Systems Direct 239-436-5000 x2188 Fax 239-436-6767 Visit our website at http://www.nchmd.org CONFIDENTIALITY NOTICE This email and any files transmitted with it are from the NCH Healthcare System. This message is confidential and is intended only for the addressee. If you are not the intended recipient or have received this email in error, please call us immediately at (239) 436-5000 and ask to speak to the message sender or promptly email the message sender of the delivery error and then delete the message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] formalin storage
I have also had 'general' safety inspectors (OSHA people who mostly focus on meat packing plants and food processors) make the same claim that we had to use metal or other cut resistant gloves. I had to demonstrate the task of cutting a frozen section to get them to understand. They still did not like it but they did recognize the problem. We do use chain mail gloves for the bone saw since it is a huge commercial band saw. Joe Galbraith University of Iowa joseph-galbra...@uiowa.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Friday, September 11, 2009 8:39 AM To: Emily Sours Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] formalin storage To prevent getting cut on the knife. Emily Sours talulahg...@gmail.com 09/11/2009 08:34 AM To Jackie M O'Connor Jackie.O'con...@abbott.com, histonet@lists.utsouthwestern.edu cc Subject Re: [Histonet] formalin storage I have to ask--what was the point of chain mail gloves?! Emily One of the defining characteristics of modern surgery was that patients ought to survive it. --Peter Stanley, For Fear of Pain: British Surgery, 1790-1850 On Fri, Sep 11, 2009 at 9:31 AM, Jackie M O'Connor Jackie.O'con...@abbott.com wrote: I once had my safety officer insist I wear chain maille gloves while cutting frozen sections. They didn' t care about all the reasons I gave them why I shouldn't - like it would be impossible to use the machine while wearing them, and the patient would have to lie on the operating table longer waiting to find out if their entire colon was going to be removed. Jean Warren jwarre...@cinci.rr.com Sent by: histonet-boun...@lists.utsouthwestern.edu 09/11/2009 08:25 AM To Riesen, Rebecca rebecca.rie...@nchmd.org, histonet@lists.utsouthwestern.edu cc Subject Re: [Histonet] formalin storage No, it is ridiculous. Safety people tried to argue this with us years ago. One of our pathologists told them, How can something that is almost 90% water be a combustion hazard? - Original Message - From: Riesen, Rebecca rebecca.rie...@nchmd.org To: histonet@lists.utsouthwestern.edu Sent: Friday, September 11, 2009 9:15 AM Subject: [Histonet] formalin storage We have been directed by our Safety Officer to store all formalin (37% and 10% NBF) in a flammable storage room, cabinet or container. Yes, 37% Formalin we do store in this manner, but I have never heard of this requirement for 10%NBF. I looked on line to many MSDS sheets from different vendors and found only one that stated such storage requirements for 10% NBF. During this search I found all but one company states that formalin is not flammable. I brought this to the Safety Officer. He agrees that it is not flammable but that it IS combustible. Combustible=Flash point of 100F to 200F. Of the dozen sites I visited I found the following data concerning the Flash Point of 10% NBF: from NA / 200F / 122F to 185F. The NFPA (National Fire Protection Agency) guideline of no more than 1 gallon in a flammable storage container and 1 gallon outside of a safety cabinet/container per 100 square feet is already quite limiting. Using this guideline, we have calculated acceptable volumes of the known flammables (Alcohols and Xylenes) we can store. Adding 10% NBF to the equation will have us traveling to our bulk storage area constantly. Does anyone out there store 10%NBF in flammable cans/cabinets? Riesen, Rebecca rebecca.rie...@nchmd.org NCH Healthcare Systems Direct 239-436-5000 x2188 Fax 239-436-6767 Visit our website at http://www.nchmd.org CONFIDENTIALITY NOTICE This email and any files transmitted with it are from the NCH Healthcare System. This message is confidential and is intended only for the addressee. If you are not the intended recipient or have received this email in error, please call us immediately at (239) 436-5000 and ask to speak to the message sender or promptly email the message sender of the delivery error and then delete the message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: Re:[Histonet] formalin storage
Additionally, we have been told that the reaction product is also a very potent carcinogen and that is the reason why you are supposed to thoroughly rinse tissue free of unbound formalin prior to immersion in HCl based decalcification agents and once again when removing from decal and placing the tissue back into formalin. Joe joseph-galbra...@uiowa.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Smith, Allen Sent: Friday, September 11, 2009 3:29 PM To: 'ti fei' Cc: 'Histonet@lists.utsouthwestern.edu' Subject: RE: Re:[Histonet] formalin storage Theoretically, the fumes of HCl can react with the formaldehyde fumes to produce bis-chloromethyl ether, which is twice as toxic as osmium tetroxide. The yield from this reaction at 1 Atm is so small that I don't think I would worry about it unless I had open containers in a closed space. Allen A. Smith Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, FL -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of ti fei Sent: Friday, September 11, 2009 10:23 AM To: Riesen, Rebecca; histonet Subject: Re:[Histonet] formalin storage never store everything in one cabinet. As far as i know, HCl and formalin should not be stored together. If any of you know why... -- Original -- From: Riesen, Rebeccarebecca.rie...@nchmd.org; Date: Fri, Sep 11, 2009 10:09 PM To: histonethistonet@lists.utsouthwestern.edu; Subject: [Histonet] formalin storage Thank you all! I have received many responses already concerning 10% Neutral Buffered Formalin (NBF) storage. Only one person has stated that they store all chemicals, including formalin, in safety cabinets. I just want to clarify something. All MSDS's I studied stated that 10% NBF indeed is NOT flammable, but the Flash Point is under 200 F on all but one manufacturer's product. The NFPA99 11.7.2.3.1 and 11.7.2.3.2 rules on flammables storage include all Class I, II and IIIA liquids. Class IIIA liquids include those with a Flashpoint of less than 200 F. That would include 10% NBF. It appears to be the formaldehyde fume (which we all know very well) is the combustible portion no matter if it is 96.7% water. Most manufacturer's recommendations are for storage in a tightly sealed container, probably to keep those nasty fumes inside. One would think that would be sufficient. Riesen, Rebecca rebecca.rie...@nchmd.org NCH Healthcare Systems Direct 239-436-5000 x2188 Fax 239-436-6767 Visit our website at http://www.nchmd.org CONFIDENTIALITY NOTICE This email and any files transmitted with it are from the NCH Healthcare System. This message is confidential and is intended only for the addressee. If you are not the intended recipient or have received this email in error, please call us immediately at (239) 436-5000 and ask to speak to the message sender or promptly email the message sender of the delivery error and then delete the message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Ink issues
I concur with the Samurai Pathologist. Long ago, we used Bouin's but have stopped that procedure and prefer to just blot dry but do also use 3% acetic acid, especially if blotting could potentially damage a delicate tissue. Good luck. Joe Galbraith University of Iowa joseph-galbra...@uiowa.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Wednesday, September 02, 2009 12:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Ink issues About what to put on tissue to make the marking ink stick to it: I don't use anything. The trick is to blot the surfaces thoroughly dry with a paper towel before you try to put the ink on. Of course, there's nothing you can use that will make ink adhere well to cauterized surfaces (some breast biopsy specimens, LEEP cones, and such. The pathologist can however identify cauterized margins under the microscope.) Because of the picric acid (and because it stains your clothes) Bouin's fixative is unacceptable. So is acetone, which is a serious explosion hazard. If you must use something, use 3% acetic acid (white vinegar diluted half-strength). I've been grossing since 1965, worked on maybe sixty different pathology services. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] uneven alternating sections on cryostat
Nathan: As indicated by others - first check that everything is tight as that is most often the issue - don't forget to check the adhesion of the specimen to the chuck as well. Also check the angle of approach to the blade as it could be too steep or too shallow. If everything is tight and properly adjusted, then you may have a retraction issue. If your cryostat retracts during the upstroke, the head may still be returning to the proper distance from the head to the specimen during the downstroke and hence you may see no section at first and then a gradually increasing thickness of section during the remainder of the downstroke. This can be due to two causes: 1) You can make this happen by operating the microtome at too high of a speed, if you slow down and the process improves then speed may be a factor; 2) Your microtome may be sticky and require maintenance (defrosting, cleaning, lubrication, etc.). The latter is best left to someone with extensive experience or a service rep. Good luck. Joe Galbraith University of Iowa joseph-galbra...@uiowa.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adam . Sent: Thursday, August 27, 2009 4:53 PM To: Nathan Cramer Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] uneven alternating sections on cryostat I was having this problem too, but I think I finally figured it out. There is a screw on our cryostat that attaches the chuck to the rest of the machine. This screws fits into a small hole in the chuck. Sometimes the screw isn't well set in the hole or is well set but for some reason comes loose and causes the chuck to wobble. For some reason, this causes your problem. Now I make sure that everything is tightly secured, and I rarely have this problem. Good luck, Adam On Thu, Aug 27, 2009 at 4:47 PM, Nathan Cramer natec...@gmail.com wrote: Thanks, Robyn... I'll make sure the holder is clamped down. (sometimes its the little things...) Best Nate R J VAZQUEZ wrote: Nathan, It sounds like the blade holder is not secure enough or even in the tightness on each side. Hope this helps. Robyn Date: Thu, 27 Aug 2009 16:44:11 -0400 From: [1]natec...@gmail.com To: [2]histo...@lists.utsouthwestern.edu Subject: [Histonet] uneven alternating sections on cryostat When cutting PFA fixed, cryoprotected tissue on our cryostat, I frequently find that every other section gets cut improperly. I'll get one nicely cut section and then on the next pass I only get half of a section. This cycle simply repeats over and over and I lose many slices. It has been a while since our cryostat has been serviced, so I'm wondering if this an operator error or a machine problem. I thought maybe the tissue temperature hadn't settled properly, but the uneven cutting still happens even when I let the tissue sit for 30 minutes in the chuck holder. (cutting mouse spinal cord at -20C) On another note, if anyone has any tips for improving white/gray matter contrast in frozen spinal cord sections stained with luxol fast blue, I'd be very appreciative. I do defat the slices with chloroform and differentiate with lithium carbonate but everything is usually either very dark or very light. I am learning as I go (checking the archives here often) so any help would be great. Thanks! Nathan Cramer Neurobiology and Behavior Cornell University Ithaca, NY 14853 ___ Histonet mailing list [3]histo...@lists.utsouthwestern.edu [4]http://lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. mailto:natec...@gmail.com 2. mailto:histonet@lists.utsouthwestern.edu 3. mailto:Histonet@lists.utsouthwestern.edu 4. http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Mouse Vs. Rabbit
Delia: Here is a link to a good overview article for the issues involved in your question. In the early days (and still somewhat true), the main difference between rabbit and mouse derived antibodies was polyclonal vs monoclonal. Today that distinction has been blurred somewhat as there are polyclonal mouse and monoclonal rabbit antibodies available for limited applications. Species selection is also driven by the need to derive an antibody in a species other than the target you intend to stain (though even that barrier has been bridged by systems like 'mouse on mouse' kits currently available). I hope this helps. http://dels.nas.edu/ilar_n/ilarjournal/46_3/pdfs/v4603Lipman.pdf Joe Galbraith University of Iowa joseph-galbra...@uiowa.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of DELIA GARCIA Sent: Monday, August 10, 2009 12:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mouse Vs. Rabbit Hi, I am new to IHC so please excuse me if I dont ask this question properly. What is the difference between a mouse clone and rabbit clone? I came across a decision I had to make while purchasing an antibody. It was mentioned that usually a rabbit clone is the preferred. Is that true and why? Thank you so much! Delia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Good Fibrin stain with Movat's?
Judy: Amos has a good suggestion. Below is a link to a Movat procedure from Penn, we discontinued our clinical Movat procedure due to lack of use (though it is a great stain). In the attached link, note the comments about the wash and differentiation steps. The Movat is nice in that it allows you to differentiate elastic, fibrin and collagen. Check after the alcian blue to see if the fibrin is already stained blue. Adjust concentrations and timing if necessary. Ensure that the alkaline alcohol is completely removed. Good luck. http://www.med.upenn.edu/mcrc/histology_core/movat.shtml Joe Galbraith University of Iowa joseph-galbra...@uiowa.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Sunday, August 09, 2009 6:03 AM To: jud...@u.washington.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Good Fibrin stain with Movat's? Hi, Have you tried a Sirius red (in sturated picric acid) with a Weigart's hamatoxylin for the Nucleii? I usually don't bother counterstaining the nucleii with this stain, but I suppose it would work. You wouuld probably want to do the hematoxylin after the Sirius red since it is an acidic solution and would bleach out the hematoxylin otherwise. While this isn't a Movat's I think it might do the trick for you. Amos Message: 2 Date: Fri, 7 Aug 2009 10:21:11 -0700 (PDT) From: Judith L. Williams jud...@u.washington.edu Subject: [Histonet] Good Fibrin stain with Movat's? To: histonet@lists.utsouthwestern.edu Message-ID: pine.lnx.4.43.0908071021110.27...@hymn33.u.washington.edu Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed Greetings to all histonetters- I have a pathologist that wants to show fibrin in lung. She wants also black nuclei and we tried a Movat's to look at fibrin. The fibrin in the lung is showing up light blue not red! Any suggestions on how to show fibrin distinctly, with black nuclei? thanks! Judy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Special stainers
We also use and like the Artisan. Joe Galbraith University of Iowa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Tuesday, July 14, 2009 9:42 AM To: kristen arvidson; histonet Subject: RE: [Histonet] Special stainers The Artisan (DAKO) is the best. Glen Dawson BS, HT QIHC (ASCP) IHC Manager Milwaukee, WI -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Tuesday, July 14, 2009 9:13 AM To: histonet Subject:[Histonet] Special stainers What stainer is everyone liking the best? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Lymphocytes IHC
Igor: CD45 is a general marker for cells of hematopoietic origin and will stain T, B and NK subsets of lymphocytes but not plasma cells. CD19 or CD20 will stain most B-cells and CD3 will stain most T-cells. Joe -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Igor Deyneko Sent: Monday, June 08, 2009 8:20 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Lymphocytes IHC Dear Histonetters! I am wondering whether there is a universal IHC marker for different types of lymphocytes, both B and T??? I am trying to start working on inflammation models and have no previous experience with inflammation markers. If anyone knows any, that would be great if they work on paraffin embedded tissues since most CD markers work on frozen only (it's for archival samples). Thank you very much in advance. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA 02139 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RPMI
Dorothy: There is a split in opinion with the pathologists on our staff but the younger and more broadly based staff (meaning multiple institution background) are adamant that the appropriate way to temporarily store tissue (not blood) is at refrigerator temp (preferably wet ice initially). I would also be interested to hear what others are saying. Joe -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Monday, June 01, 2009 12:44 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RPMI What is the correct way to store RPMI after placing a tissue sample in it, refrigerated or ambient? Thanks for your educated assistance!! Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Microtome Problems
Sharon: I will let others with personal experience with your particular microtomes offer advice on how to repair them but, first and foremost, if these problems occurred as a direct result of the service call, I would be in immediate contact with the company that provided the service, complain to them about the outcome you experienced, and insist that they make the situation right by you. Especially if you contact them right away, they should at the least return to correct the problems they created, possibly by sending a different rep. Best of luck. Joe -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Veterinary Services Laboratory Sent: Friday, May 29, 2009 9:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome Problems Hello All I'm new to the group and histo. (3 years) so far I'm getting a lot of useful tips from all the messages. Thank you. I have two microtomes that were working almost perfectly before I had a technician service them. Now, the Leica 2035 Jung biocuts' blade holder is twisting to the side (going up at one end). I have tried tightening, slacking, and cleaning the entire assembly to no avail. Does anyone know of where I might be able to source one or even how to fix the one I have. Secondly, the Leitz 1512 rotary microtome, is now jumping forward and giving a loud clanking noise. It takes huge chunks out of the tissue. Any and all help is appreciated, Thank you Sharon Ms. Sharon Drayton Veterinary Services Laboratory Ministry of Agriculture Rural Development The Pine St. Michael BB11091 Barbados Tel: (246) 427-5492 or (246) 427-5073 Fax: (246)426-7517 Email: vet...@caribsurf.com Website: www.agriculture.gov.bb ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Dry ice - transportation
Richard: There may be local regulations that could vary. Here you can buy dry ice in the grocery store (if you bring an appropriate container). The safety warnings associated with dry ice indicate (as you would expect) - transport in unsealed insulated container (Styrofoam chest), transport in the trunk of your car not the passenger compartment and crack a window, avoid direct contact with skin eyes or ingestion to avoid burns, etc. If your PA's are traveling long distances using a minivan SUV or similar single compartment vehicle, the main problem would be accumulation of CO2 gas that would ultimately induce drowsiness and in worst case scenario asphyxia. Joe -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, May 21, 2009 1:33 PM To: Histonet Subject: [Histonet] Dry ice - transportation Are there any restrictions on the transportation of dry ice (and human tissue) in private vehicles? Our PAs cover a surgical center off-site and we need to transport frozen tissue back to the hospital for biobanking purposes. They drive back and forth in their own vehicles. Thanks. Richard Richard W. Cartun, Ph.D. Director, Histology Immunopathology Director, Biospecimens Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Leica cryostat
Janet: I've worked with the CM1850 and 1950 and like them a lot. You listed the 1800 twice. Was one of your options an 1850? Joe -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dertien, Janet Sent: Wednesday, May 13, 2009 3:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica cryostat Hello everyone, We have decided to purchase a refurbished Leica cryostat and are trying to decide which model to choose; does anyone have experience with the 1510, the CM 1800 or the 1800? Any feedback would be appreciated. Thanks! Janet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Semiautomated Antigen Retrival systems/equipment
Hello all: Is anyone using any standalone semi-automated antigen retrieval equipment? I've seen a few microwave or wet bath based systems (sometimes integrated into full blown IHC staining systems) in the lit and on the net. How many people are still using the Nordicware pressure cooker in a microwave or the BioCare Medical electric antigen retrieval pressure cooker? Comments are welcome as we are looking at short and long term solutions to make the IHC process less labor intensive. Thanks. Joe ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] FW: prover' pojaluista
We have found that the key to IHC staining consistency following HIER (esp. when using a pressure cooker) is consistent and SLOW cool down. Opening the cooker too quickly can cause flash boiling and is dangerous but removing the slides from the cooker too rapidly and plunging them into room temp buffer can also be problematic. We found that careful trickling of DI water into the cooker for a consistent amount of time until the buffer reaches ambient temp to be effective. We actually time the cooling process and check the temp to be sure the slides are gently and adequately cooled before transferring to buffer prior to staining. Joe Galbraith -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, May 07, 2009 11:57 AM To: histonet@lists.utsouthwestern.edu; Margaryan, Naira Subject: Re: [Histonet] FW: prover' pojaluista Strictly speaking, IHC reactions do not depend on water temperature temperature. What you describe does not include dist. water between the IHC steps therefore the water temp. should not be affecting your reactions. If you use dist water after HIER you have to remember placing the slides into room temp. buffer before starting the IHC procedure, so if there was any effect due to the dist. water, that should have take care of it. The inconsistency in your staining I think is not due to the dist. water temp. René J. --- On Thu, 5/7/09, Margaryan, Naira nmargar...@childrensmemorial.org wrote: From: Margaryan, Naira nmargar...@childrensmemorial.org Subject: [Histonet] FW: prover' pojaluista To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Thursday, May 7, 2009, 11:23 AM Dear Histonetters, This is my question regarding IHC staining and water temperature: does IHC staining depend on water temperature in washing step? As always, I am using the same protocol for IHC. But one day it works perfectly, another day, on the same tissue, it does not work. Everything is same, solutions are fresh, and the only thing that is different is water temperature. I am using running distilled water before and after Antigen Retrieval step, after DAB, after counterstaining and after bluing reagent. Dd-water goes into autostainer: all blocking steps and after primary, secondary and tertiary. I am not using usual running water to exclude different Chlorine concentrations in different days. Have anybody of you ever paid attention to running distilled water temperature? What water temperature (cold or warm) you will recommend for washing steps? If this is critical, I can prepare my water up front. Hope to get an answer soon. Thanks, Naira ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Snap-Frost System
Hello:
Does anyone have any experience with using the Snap-Frost rapid freezing
system from Alphelys (France)? Our application is predominantly for
tissue banking though we do a booming business in intraoperative
diagnosis as well. The company and one review article {Virchow Arch
(2008) 452:305-312} claim good RNA/DNA recovery from OCT embedded,
isopentane frozen tissue using the system. They also claim good to
excellent morphology at -80 in isopentane compared to liquid nitrogen at
-190 and especially compared to CO2 freezing. Can anyone confirm or
refute the claims? Does anyone know of a US based supplier of the
Snap-Frost system? Thanks.
Joe Galbraith
University of Iowa Tissue Repository
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