Re: [Histonet] Tape Transfer Methods For Cryosectioned Brains
Hi Paula, I am cutting at -24, but have tried going as warm as -18. I am currently learning with 30uM sections with the ultimate goal of moving towards 5 or 10uM. Our lab standard has been collecting into millonigs solution and doing free floating IHC. We generally have no issue with this technique, but do lose some of the peripheral damaged tissue near the infarct in our stroke brains. We're trying to work up painting the samples directly onto slides and skipping free floating staining to get a better end product. My current samples are very old, they were collected into 4%PFA in 2017 and cryoprotected by freezing in OCT cryomatrix in a little dish floating on LN2 in 2019. They've been stored at -80 since then. Usually we process the brains within a few weeks of collecting them so this particular tissue isn't our ideal situation. We're using old tissue to practice technique, so the current samples aren't going to be used for any actual analysis. When we heard about the tape method of collecting we were very curious as the the opinion other labs have about it. Have you tried it? Thanks for the answer! Heather Heather Deziel, MSc. Laboratory Technician, CNS|CRO 550 University Ave, Charlottetown, PE C1A 4P3 (a subsidiary of Neurodyn Life Sciences Inc.) On 2021-04-15 10:29, Patpxs wrote: > Good Morning Heather, > > I have some questions about how you cut frozen brains. > > What temperature are you cutting at? > How thick are your sections? > How are your samples frozen? Flash freezing, slow freezing, iso-pentane in > LN2? > > Your answers may provide clues to help you get better cryosections. > > Paula > > Sent from my iPhone > >> On Apr 15, 2021, at 5:39 AM, Heather Deziel via Histonet >> wrote: >> >> Hello Histonet, >> >> I'm looking into working up a tape transfer method of collecting >> cryosections of brain while preserving infarct to be used in IHC. I >> find that when I try and section heavily damaged regions of the brain >> the tissue tears and and I lose it. Has anyone got any recommendations >> about the the Section-lab transfer tape (Kawamoto method), using the >> circuit plating tape recommended here >> (https://www.future-science.com/doi/full/10.2144/btn-2018-0021) or the >> cryojane system from Leica? >> >> Thank you, >> >> Heather >> >> Heather Deziel, MSc. >> >> Laboratory Technician, CNS|CRO 550 University Ave, Charlottetown, PE C1A >> 4P3 >> >> (a subsidiary of Neurodyn Life Sciences Inc.) >> ___ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tape Transfer Methods For Cryosectioned Brains
Hello Histonet, I'm looking into working up a tape transfer method of collecting cryosections of brain while preserving infarct to be used in IHC. I find that when I try and section heavily damaged regions of the brain the tissue tears and and I lose it. Has anyone got any recommendations about the the Section-lab transfer tape (Kawamoto method), using the circuit plating tape recommended here (https://www.future-science.com/doi/full/10.2144/btn-2018-0021) or the cryojane system from Leica? Thank you, Heather Heather Deziel, MSc. Laboratory Technician, CNS|CRO 550 University Ave, Charlottetown, PE C1A 4P3 (a subsidiary of Neurodyn Life Sciences Inc.) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Mounting medium
I’m trying to find a really rapid drying mounting medium for same day shipping does anyone have any suggestions? When reading they usually just say “rapist dry” but how long is “rapid” Thanks for the help awesome histopeeps! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thank you for input
Thank you to everyone for the recommendations for xylene alternatives. Stat lab is trying to make it right and I have a lot of options to explore now. Thanks Heather ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] AM to statlab and recommendations
Did anyone else use American Mastertech and is now super frustrated with Stat Lab??? I’ve used AM for years and loved them, great prices and fast shipping, now cost is through the roof and they’re shipping all my stuff separately costing a fortune! Looking for recommendations on xylene alternatives and safe mounting medium. I’ve been using transcend and cover safe two American Mastertech signature products but I’m running for my life. I’ll be contacting a few of the other vendors I use as well but I’d like some recommendations on xylene alternatives we’ve been xylene free for 4 years and I’m not going back. Thank you Heather ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Seeking On call Histotech
Hello Histonet! We are seeking an “on call” histotech to help us through our busy season and potentially longer. Our lab is located in Spokane Valley, Wa and it’s a fun relaxed environment. The schedule is very flexible and pay negotiable. We are a veterinary and research focused lab but welcome all backgrounds. It’s a very unique position with a lot of growth opportunity. Please send resumes to nationwidehistol...@gmail.com Thank you, Heather Marlatt ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Histonet Digest, Vol 170, Issue 13 histology hacks
Hey Everyone!! I haven't read this book yet, but we are planning to buy it for our department. I can see that a lot of remarks and reviews are being made and I wanted to give some points from someone who was coming into the field from having a B.S. and several years of training as a lab assistant. I have now been in histology for 11 years, but have only been certified for 6. 1. Congratulations on the book! I think that it is something that will be useful for new techs. 2. Hack is a word that is generally known in the younger generations as a way to fix things or make things that you wouldn't normally think of. There are many labs out there (I have worked at two) where the physicians and/or pathology administration wouldn't do anything to have the tissue processed in a better way. For example: Fatty tissue being cut in WAY too thick. Things being crammed into the cassettes so it was difficult to fit it into the mold. Staples, sutures, etc. not being removed from the tissue during grossing. As a tech, there is not a whole lot that can be done from our perspective to get things changed. We can mention it, but you won't always be in a situation where those changes will be made. (We lived by the motto "Crap in, crap out", but you like to be able to do the best work possible, especially since we are dealing with patient tissue. This is where techniques would be helpful to employees-so that you can do the best work possible with what you are provided. We used to cut most of our fatty blocks in cold water and lay the slide gently on the water bath to remove the wrinkles. New techs are not going to know these tricks and let's face it, most of the people who know these tricks/hacks are about to retire. So let's not judge or be critical of someone who is trying to make the field more user-friendly. Side note: Thank-you everyone who asks and responds to questions on here! I have learned a lot and I think that it is important for us to keep an open dialogue to learn from one another! HEATHER SEELEY, HT(ASCP) From: Steve McClain [ste...@mcclainlab.com] Sent: Tuesday, January 16, 2018 7:24 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Histonet Digest, Vol 170, Issue 13 histology hacks I purchased the book and applaud the effort because there is some decent information. However, the term hack is a poor choice for histology and many of the fixes or secrets described are because some hack failed to do her/his job at an earlier step in the process. (Definition of hack. transitive verb. 1 a : to cut or sever with repeated irregular or unskillful blows. b : to cut or shape by or as if by crude or ruthless strokes. As a noun it is used to mean a mediocre performer or worker; tiresome drudge.) Some methods, while useful in some settings, have important cons not listed, cons which may be counterproductive. For example using Mercurochrome or Eosin to mark tissue may preclude further testing with fluorescent endpoints, such as FISH. Plus if you really want to use Eosin to mark the dermis, it is far easier to add used Eosin to one alcohol in the tissue processor. That gives a visible indicator of carryover, indicating need to change or rotate solutions. Other methods seem (to me) like workarounds or Band-Aids for Labs w poor grossing, poor processing or poor reagents or poor technique or poor method choices, eg, 2.14 describes a situation where an incompetent grosser truly hacks or crudely cuts into unfixed tissue yielding too thick a slice. The real solution is to fix the tissue before slicing. Poor fixation results in poor processing and poor sectioning and poor staining reactions. For another example, Cassette sponges offer few advantages, while folding lens paper allows the grossers to see through the paper and know all pieces are inside before closing the cassette lid. The tissue does not stick to it, and small flakes can be scraped from the lens paper at embedding. Last during folding, the forceps can be cleaned at grossing and during unfolding, forceps may be cleaned w the paper after embedding. Sponges also result in greater solution carryover. Several colloquial naming conventions, eg, chamber saver 2.16 for underprocessed tissue may be memorable to some readers, yet seem are odd to me. This 2.16 method is an especially useful technique which may also be done to extend paraffin time, whenever poor sectioning due to poor processing is encountered at the microtome. Variation 1 Place the block back into the proper sized mold and return to the heated side of the embedding center for an hour to extend processing (reprocessing). Then remove the old paraffin from the mold w a plastic pipette, then re-embed, replacing the paraffin w new. Variation 2 for outside blocks we routinely replace an unknown paraffin from another lab by melting in a mold, and ‘reprocess’ in our (b
[Histonet] Mouse bladder
Does anyone have a good protocol for mouse bladder embedded in agar that they are willing to share? We have mouse bladders in 2% agar and have tried a few different protocol variations from our usual mouse but the agar keeps shriveling up. Thanks Heather ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FT Days Rock Hill, SC
Hey Everyone!! We have a position opening up and I wanted to let everyone know in case there is any interest. We all work FT M-F. There will be three other techs, so four total with the new employee. There are three pathologists, two transcriptionists, a PA, and two cytotechs and we are like family in here! We do monthly fun lunches as well as a lunch club, Secret Sister, and we even do artsy things sometimes on weekends. We are looking for someone with a positive and energetic mindset that will fit in, but also someone with good work habits and some experience. We would love to hear from you! Please contact me directly at heather.see...@tenethealth.com<mailto:heather.see...@tenethealth.com> for more info! HEATHER SEELEY, HT(ASCP) Histotech 803-985-4676 OFFICE 803-327-7598 FAX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] A Question About Paraffin Times
We do a delay protocol as well. The tissue samples will sit in formalin and start processing Sunday night for a normal amount of time to come off Monday morning. If this is not a possibility, it would be better to let them harden in the paraffin than to leave them in the hot paraffin, as this will lead to the tissue becoming brittle and very difficult to section. HEATHER SEELEY, HT(ASCP) Histotech 803-985-4676 OFFICE 803-327-7598 FAX From: P Sicurello [pat...@gmail.com] Sent: Tuesday, May 16, 2017 11:34 AM To: HistoNet Subject: [Histonet] A Question About Paraffin Times Good Morning Listers, I am asking the collective wisdom of the Histonet this question: Is it better to remove baskets from the processor on Saturday morning and: A. Let the cassettes freeze, then melt them down and embed Monday morning? OR B. Leave the cassettes in molten paraffin and embed Monday morning? I am of the opinion that leaving the samples (not fatty, like breast cores) in molten paraffin (62 degrees C) is bad practice, and causes them to get "crunchy", among other things. What do you think? Thank in advance. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 <#> *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Dirty HP Antibody
We also had the same problem with Ventana. We are currently using Cell Marque and it is working great! HEATHER SEELEY, HT(ASCP) Histotech 803-985-4676 OFFICE 803-327-7598 FAX From: Terri Braud [tbr...@holyredeemer.com] Sent: Monday, May 30, 2016 12:19 PM To: 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Dirty HP Antibody We had the same issue a few years ago with the Ventana HP antibody. Then we tried the antibody from Zymed and it was so much better. Now we use BioCare's antibody, and it is very clean. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] PAS Stain
We have one girl that always spits on our slide! :) works great! HEATHER SEELEY, HT(ASCP) Histotech 803-985-4676 OFFICE 803-327-7598 FAX From: Bob Richmond [rsrichm...@gmail.com] Sent: Thursday, May 05, 2016 2:10 PM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] PAS Stain Amylase (diastase) for the PAS stain queries: Whatever happened to spitting on the slide (30 min at room temperature)? John Kiernan advises "thinking of lemons and drooling into a small beaker" though I'd advise chewing on a rubber band for a few seconds. He notes that alpha amylase is preferred. I'd go with the cheapest one in the Sigma-Aldrich catalog. Room temperature is usual, but I note that Sigma offers a heat-stable alpha amylase. Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Blog Post Not lab related
I am new to the Histonet, not to histology though. There have been 2 great question and answer sessions since I've been on here. The way some of you guys talk to each other is so disrespectful. I went through AFIP as a civilian years ago and I thought the histo world was a small society of people who shared a common, if not morbid, curiosity of the human anatomy, how it works and how can we do the best we can do to help our patient. I will read all the submissions, because there may actually be a good answer to a question. I will have to really weigh my options with asking a question...cruelty and ridicule or the possibility of a good answer? If you don't like histo, then don't do histo. If you can't be nice, then say nothing at all. Life is a beat down everyday, I darn sure wouldn't need if from my peers too. Heather L. Brown, HT, ASCP JBSA-Lackland, Texas ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Lab Coats and OCT
Hello All, Now that the hospital is enforcing us to wear lab coats all the time, wondering if anyone has suggestions for some that aren't too hot, the ones we have now are making us sweat! Also, we have been having issues with our OCT, we currently use the Sakura OCT and have use it for years. Within the last 6 months or so it has become like glue on the chucks even if we let it soak in hot water and scrub with a brush, it just doesn't want to come out! If anyone has any suggestions on brands to try we would appreciate it! Thanks! HEATHER SEELEY, HT(ASCP) Histotechnologist 803-985-4676 OFFICE 803-327-7598 FAX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PPE/ Hair Regulations
Hello All! Wondering what your lab requires for histology as far as lab coat length and hair policy? We have a bit of a controversy going on and was wanting to get some feedback from fellow histotechs! We are in a hospital and the histology department is located within the lab. Thanks, HEATHER SEELEY, HT(ASCP) Histotechnologist 803-985-4676 OFFICE 803-327-7598 FAX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fulltime Opening LaCrosse, WI
Hi All, Gundersen Health System located in LaCrosse, Wisconsin has a fulltime Histology Technician position available. Go to http://www.gundersenhealth.org/careers for position description and information on how to apply! Heather Laudon, BS, HTL (ASCP)cm Histology Technician Surgical Pathology Laboratory Gundersen Health System 1900 South Avenue Lacrosse, WI 54601 Mail Stop: H04-008 (608)775-3139 hmlau...@gundersenhealth.orgmailto:hmlau...@gundersenhealth.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] OJT Histotechs/Training
I agree and would also like to like Pams response. On May 14, 2015 1:26 PM, Joelle Weaver joellewea...@hotmail.com wrote: I know it is hard Hazel. I have a hard time finding well qualified people myself. OJT only works now if you already have a degree. If we can't raise the pay and the recognition we will have some difficulty recruiting those people too. I know the situation well, as I used to be the sole educator person in an HT program. It closed due to lack of enrollment and funding. I have trained people the best way that I knew how both formally and informally. I have driven in my car to multiple states,written articles, gone to conferences, schools, colleges and trade shows, and did my personal best to present topics and sell' our profession. Admittedly, sometimes it feels like it doesn't matter or change anything. But we have to keep trying and pushing for our group, its best interests, its public recognition, its compensation. There will be no budging from anyone else I'm afraid. Change often moves like a glacier, but it does move! Joelle Weaver MAOM, HTL (ASCP) QIHC From: hor...@archildrens.org To: joellewea...@hotmail.com; timothy.mor...@ucsf.edu; histonet@lists.utsouthwestern.edu Date: Thu, 14 May 2015 14:46:40 -0500 Subject: RE: [Histonet] OJT Histotechs/Training Joelle I agree with you. But the problem is, no one knows we exist. OJT is the only route for some/if not most positions to be filled. We would all love to have a choice of educated ASCP registered techs to choose from. I have an open position and no applicants. Hazel Horn, HTL/HT (ASCP) Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.org archildrens.org -Original Message- From: Joelle Weaver [mailto:joellewea...@hotmail.com] Sent: Thursday, May 14, 2015 2:35 PM To: Morken, Timothy; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] OJT Histotechs/Training We have discussed this on the histonet many times... Most professions, and most if not all healthcare professions, require degrees and/or certification for entry. This is how the public, other medical professions ,and even HR-who do not know the technical- assess for our capacity to provide care and judge the skill level needed of the profession as they are looking in. We all know that this isn't always perhaps the best method to assess or measure some aspects of this profession, but this is what they work from. There are good and bad examples of both OJT and educated, formally trained histology professionals. However, education is more than learning facts, it helps develop many other facets of the person that are viewed as valuable to organizations. That is why it is used as a screening tool. Please try to value the broader perspective. Technical proficiency itself is probably not going to be enough as the future unfolds. Though it may seem unfair if you have worked for a very long time and learned a great deal through experience, the bottom line is that for some employers, some environments and outside groups- education, credentials and professionalism are the primary criteria they use to evaluate, and they pay and recognize accordingly. Joelle Weaver MAOM, HTL (ASCP) QIHC From: timothy.mor...@ucsf.edu To: histonet@lists.utsouthwestern.edu Date: Thu, 14 May 2015 17:28:15 + Subject: Re: [Histonet] OJT Histotechs/Training Mike, yes, the vast majority of histotechs have been, are, and will be OJT (me included). The people who take on training these people have a responsibility to do the best they can. Most techs end up learning whatever their lab does and so have limited knowledge. I studied a full year for the HT and passed fine, and later the HTL. In our small lab at the time we had a broad array of testing in histology (specials, muscle histochem, immunochem, electron microscopy), but I found out my true lack of knowledge when I went to Saudi Arabia and worked with techs from other countries where they had comprehensive bachelors-level programs required for ALL lab techs. Those from the US, all certificated, where vastly under-educated compared to techs from other countries. It was a bit embarrassing! Luckily we have online courses and degrees available now - not available in the 1980's when I started. That is a tremendous advantage to those who are willing to take advantage of it. Other than that it will be up to the lab management to be sure the OJT tech gets the basic instruction according to the requirements of the ASCP exam. That is the bare bones knowledge necessary to function. Even then the experience in the lab is key to whether the knowledge is just regurgitated or practiced. Lab management has a responsibility to be sure good lab
[Histonet] C1q FITC
Does anyone have a vendor with C1q FITC availability? We typically order Dako product #F0254 which is a polyclonal rabbit anti-human C1q complement, but are told they are on backorder for an undetermined amount of time (possibly May 2015). Thanks for your help! Heather Laudon, BS, HTL (ASCP)cm Histology Technician Surgical Pathology Laboratory Gundersen Health System 1900 South Avenue Lacrosse, WI 54601 Mail Stop: H04-008 (608)775-3139 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Bcl-2
Hi everyone- Just wondering if anyone has a working protocol for Bcl-2 in FFPE mouse tissue? If so, can you share both the protocol and the antibody information? We have tried numerous antibodies over the years with very limited success. Thank you for your help!! Best, Heather Knight ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Control Blocks
Anyone know where I can purchase positive control blocks for special stains? I'm in an independent lab and don't have access to hospital cases. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Plant Pathology
Do any of you knowledgeable Histo-folks work with plants? I have a researcher that wants to use FISH probes on some plant samples and I have never prepared plant tissues. If anyone out there has some tips/protocols/recommendations I would really appreciate it. Thank you! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] See You Soon
CONGRATULATIONS SARAH! I hope you love the new position! :-) On Wed, Nov 27, 2013 at 11:13 AM, Sarah Dysart sdys...@mirnarx.com wrote: Hey guys and gals...I have taken a new position as Pathology Supervisor at North Austin Medical Center here in Austin...so I am unsubscribing for now, but have no fear...I will be back ASAP with my new email address. Hope everyone has a great Turkey Day!! Signing off for now... Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Question - OR specimens to Pathology
I wouldn't recommend putting fresh tissue specimens destined for frozen tissue processing into or on formalin before it is grossed, etc. Our Mohs surgeon walks the surgical specimen from the OR directly to the Mohs lab on an oriented piece of gauze in a clean petri dish along with map, chart, etc. Surgeon and tech then have the opportunity to discuss specimen before tech processes. This process passes muster for CLIA and works well for us. Heather D'Orazio Mohs Tech Rogers Dermatology Clinic 1727 West College Bozeman MT 59715 (406) 587-4432 From: Grantham, Andrea L - (algranth) algra...@email.arizona.edu To: Cc: HISTONET histonet@lists.utsouthwestern.edu Sent: Thursday, August 1, 2013 3:51 PM Subject: Re: [Histonet] Question - OR specimens to Pathology OR - you can get a rubbermaid took box and slap a few biohazard stickers on it and put some formalin pads inside and you have a transport box for surgical specimens. I happen to know that that is how this box was invented. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edumailto:algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Processing Guinea Pig
Hello Histonet! I'm a long time reader first time poster. Does anyone have experience processing guinea pig tissues? I have been processing kidney and heart but it is consistently coming out mushy in the middle. The mouse tissue comes out fine even when processed on the same run. I had the tissue grossed in thinner (2.5mm) thinking that perhaps it was too thick but it didn't seem to help. Also, it has been fixed in 10%NBF for several days. I was just wondering if anyone else had similar problems with guinea pig? I appreciate in advance any advice or tips. Here is the protocol: Formalin 1hr 70% etOH 1hr 95% 1hr 100% 30min 100% 1hr 100% 1hr 100%1hr Clearify 1hr Clearify 1hr Clearify 1hr Paraffin 1hr paraffin 1hr All under pressure and heat only on the paraffin. Thanks Heather ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] vacuum sealer
Sipromac table top vacuum sealer - we have the 350 model. http://www.sipromac.com/a_p01a.html -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- boun...@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Monday, December 31, 2012 2:39 PM To: Sarah Dysart; Bernadette del Rosario; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] vacuum sealer The only vacuum system I have experience with is the Kapac vacuum bag system. There are various bag sizes to choose from, and the bags themselves are quite thick. I must also put out there, that after sealing, even doing it twice (two seals) on each bag, and storing, we have experienced leakage after a few years. When going back through for either retrieval or discard, we run across ~10% that have leaked out and the tissue has dried up. :( Would be interested in seeing if there is a product out there that is better that I missed out on! Happy New Year everyone!! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 From: histonet-boun...@lists.utsouthwestern.edu [histonet- boun...@lists.utsouthwestern.edu] on behalf of Sarah Dysart [sdys...@mirnarx.com] Sent: Monday, December 31, 2012 9:31 AM To: Bernadette del Rosario; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] vacuum sealer Get one from Walmart. Half the price and last just as long =) Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- boun...@lists.utsouthwestern.edu] On Behalf Of Bernadette del Rosario Sent: Sunday, December 30, 2012 9:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] vacuum sealer Hi histonets..Im looking for a good brand of vacuum sealers for storing histology tissue samples.Dont you have any issues with this sealers.Im looking for one which retains a little formalin in it.I want to know which type you are using..Thanks a lot.Have a great day.. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Modified Gomori Trichrome
I have request to implement this stain for frozen muscle in OCT blocks. The pathologist is looking for demonstration of ragged red fibers, however it is my understanding (very limited) that this (in this circumstance) is a diagnostic stain and the tissue on hand that I will be using for development will be normal with no RRF present. I am unable to discern what type of tissue to use as a positive control, outside of muscle diseased specimen. Any suggestions and/or working methods would be greatly appreciated. Heather Hart, MLT (ASCP) This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Slide Baskets????
I was wondering if anyone in histoland has any slide baskets for the old sakura DRS 601 stainer laying around??? I can't seem to find these for a decent price so I thought I'd ask. I only have one at the moment and as you might imagine that us really cramping my style. Thanks in advance Heather ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Reagent containers???
Does anyone have reagent containers and slide baskets for a Shandon LTD Varistain XY Slide stainer that they would be willing to get rid of? Feel free to reply to my personal email addy Thanks Heather ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HTL exam
Hi, I am taking the HTL exam in about three months and would really appreciate any guidance that anyone has, study tips, etc. I have already downloaded the ASCP information on the test. Thanks! -Heather ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microwave use in histo lab
Hello everyone! I am currently taking a class titled Current Trends Applications in Applied Science Technology which is essentially a capstone course for my BSAST degree completion. This course requires a four part research paper on any technology of choice in the (students) related field. The topic I have chosen is Microwave Use in the Histology Lab. I am trying to gather information for the third module in which I need to address the topics listed below. I would appreciate any personal input or opinions about the topic per guidelines listed. Thank you for your help! Heather Hart, MLT (ASCP) Various Perspectives and Opinions Provide alternative perspectives from experts on technology Political implications and influences Public opinion on technology such as the media, consumers and community Your assessment of how effective the initial planning and risk assessment was to the implementation and usage of the technology “You are encouraged to access other users and/or associated technologists in order to gain insight into various perspectives and fully understand the applications of the technology selected.” This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] re: april fools prank
I've been known to leave a fake spider in an embedder for the morning person.although it wasn't april fools just for fun it got a great reaction :) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] hrp vs alk phos
Dear Histonetters, Our pathologist wants us to change from HRP to ALK PHOS. We run a Ventana XT using the titration option. Fifteen of our 20 antibody selection are giving the same result when run concurrently with the 2 detection systems. The other 5 stain perfectly with the HRP kit and gives NO staining with the ALK PHOS kit. We use the same protocol except for the kit selection and the titrated antibody is taken from the same vial for the respective pairs. Has anybody had a problem like this? The answer may be obvious but we cannot see it . yet. Please help Many many thanks Heather On Thu, 2 Feb 2012 15:31:04 +0100 Gudrun Lang gu.l...@gmx.at wrote We have been performing C4d IHC on FFPE kidney for a couple of years. We receive only fixed samples. Yesterday my doctors came with this idea of IF - I still have to ask for the special reasons. But I have the suspicion, that they are not aware of the fact, that IF on frozen unfixed tissue is the usual way found in literature. There are some articles that deal with comparison of IF(frozen) and IHC(ffpe). The results are usually an equal outcome. IF(frozen) shows additional staining in glomeruli. Perhaps someone told them, that IF is the golden standard and recommended. Gudrun -Ursprüngliche Nachricht- Von: Richard Cartun [mailto:rcar...@harthosp.org] Gesendet: Mittwoch, 01. Februar 2012 21:21 An: gu.l...@gmx.at Betreff: Re: [Histonet] C4d IF on FFPE kidney Hi Gudrun: Is there reason why you want to use IF and not immunoperoxidase? Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Gudrun Lang gu.l...@gmx.at 2/1/2012 3:17 PM Hi! Can someone provide me a immunofluorescence protocol for C4d on formalin fixed human paraffin sections? thanks in advance Gudrun Lang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet South Africas premier free email service - www.webmail.co.za For super low premiums, click here. http://www.dialdirect.co.za/?vdn=15828 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Decal for IHC
Can anyone offer a recommendation for a good decalcifier that does not comprimise Immunohistochemistry results...in particular ER/PR? Thanks for your help! Heather Laudon, HTL Histology Technician Surgical Pathology Laboratory Gundersen Lutheran Hospital 1900 South Avenue Lacrosse, WI 54601 Mail Stop: H04-008 (608)775-3139 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Bone Marrow Biopsies
What is the procedure for collecting and processing bone marrow bx? How much time should it be placed in DECAL?? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CLIA requirements for Profiency Testing
Getting ducks in a row for CLIA inspection, and saw proficiency testing on thier checklist. We are a dermatology inhouse lab, anyone doing PT testing in same situation? If so, who do you use? Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Does anyone have a good protocol for Giemsa? I really need it ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Old VIP cassette baskets
Does anyone know where I can find a basket that was used in the older VIP processors? Its about 4.5 x 5 inches, holds maybe 50 cassettes laying down on their side. I'm not familiar with where to find used Histology equipment. If someone has one for sale I would love to hear from you. Thanks Heather ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Procedure Manual Template
Does anyone know of a standard operating procedure manual template could be found? I have to write one and need a little guidance. And thanks to all who have helped with my other questions, I cant figure out how to reply to the old posts... Thanks again Heather ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Poor contrast in HE stain
I am in the process of setting up stain run for our new stainer and am having a problem getting crisp contrast in our HE. I have adjusted times with little improvement. The stain looks muddy I guess. The pathologist says it's the contrast. We are doing skin biopsies. Using Xylene, Gills, richard allen clarifier , doesnt seem like this should be too hard, but it's getting frustrating. Thanks HR ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Processors and power outages
Wondering what the labs who are not in hospital setting, are doing with thier processors for power outages. Starting a derm lab and want to address this issue. Thanks H. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HT Exam
Hello everyone! Has anyone taken the HT Exam recently. I heard it was hard. Can anyone give me any help please. Heather Cooper TruPath Laboratory, LLC Leesville, LA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HELP!!!!! H. Pylori Immunos
Does anyone do H. Pylori Immunos by hand? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Could you please forward this job announcement to the list serve: VA Medical Center, Loma Linda, CA is seeking applicants for a Histology Supervisor. The department consists of three histotechnologists and two cytology/histology technicians and has fully automated immunohistochemistry, special stains, HE stains and cover slipping. The responsibilities of this position include supervising the daily operations of the histology department including staff training, competency assessment, performance reviews, maintenance of and adherence to standard operating procedures, routine quality assurance, adherence to CAP and JCAHO standards, and selection and implementation of new instrumentation and equipment. This position is a working supervisory position, and it is expected that the supervisor will regularly perform bench work including embedding, microtomy, special stains and immunohistochemistry, with certain days set aside solely for supervisory duties. Applicants should have supervisory experience, solid interpersonal skills, strong immunohistochemical technical skills, knowledge of CAP and JCAHO regulations, good written communication ability, and a solid knowledge of histology. ASCP Histology Technician certification and US Citizenship are required. Position is subject to random drug testing. This agency provides reasonable accommodations to applicants with disabilities where appropriate. For further information from Dr. Heather Rojas at (909) 825-7084 x2586 or by email: heather.ro...@va.gov Qualified candidates may send resume to: Anna DeRito, HR Specialist (136-05S) Jerry L. Pettis VAMC 11201 Benton Street Loma Linda, CA 92357 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] histonet post
Could you please post the following announcement: VA Medical Center, Loma Linda, CA is seeking applicants for a Histology Supervisor. The department consists of three histotechnologists and two cytology/histology technicians and has fully automated immunohistochemistry, special stains, HE stains and cover slipping. The responsibilities of this position include supervising the daily operations of the histology department including staff training, competency assessment, performance reviews, maintenance of and adherence to standard operating procedures, routine quality assurance, adherence to CAP and JCAHO standards, and selection and implementation of new instrumentation and equipment. This position is a working supervisory position, and it is expected that the supervisor will regularly perform bench work including embedding, microtomy, special stains and immunohistochemistry, with certain days set aside solely for supervisory duties. Applicants should have supervisory experience, solid interpersonal skills, strong immunohistochemical technical skills, knowledge of CAP and JCAHO regulations, good written communication ability, and a solid knowledge of histology. ASCP Histology Technician certification and US Citizenship are required. Position is subject to random drug testing. This agency provides reasonable accommodations to applicants with disabilities where appropriate. For further information from Dr. Heather Rojas at (909) 825-7084 x2586 or by email: heather.ro...@va.gov Qualified candidates may send resume to: Anna DeRito, HR Specialist (136-05S) Jerry L. Pettis VAMC 11201 Benton Street Loma Linda, CA 92357 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IgG4 source
I have used invitrogen - mouse anti-human IgG4 Clone HP6025 (successfully) AR in 1mm EDTA Dilution 1:400 Control - tonsil (or Sjogren's Syndrome) Joao Pessoa jphistol...@gmail.com 2010/05/07 11:50 PM I need to find a source for an IgG4 antibody for use with IHC studies on human FFPE tissue. Any ideas? My preference would be for a concentrated IVD. Thanks, Joao Histo Tech ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 4500. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. _ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Previous Technician of mine Looking for a position in Wilmington, NC
Hello All, Posting this for an old traveler/technician of mine: She is looking for a position geographically based by or in Wilmington, NC. If you know of any leads pass them along and I will forward to her. Thank you in advance! Heather D. Renko, Histology Supervisor OSF Saint Anthony Medical Center Main Laboratory-Histology 5666 East State Street Rockford, Illinois 61108 815-395-5410 Direct 815-395-5116 Department == The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. == ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] re: H. Pylori
We are very pleased with the Ventana pre dilute and if we purchased controls, I would recommend Master Scientific as we have always been very pleased with them as a whole. Heather D. Renko, Histology Supervisor OSF Saint Anthony Medical Center Main Laboratory-Histology 5666 East State Street Rockford, Illinois 61108 815-395-5410 Direct 815-395-5116 Department == The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. == ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Leica Cassette Printer
We have the Leica cassette printer in our facility too. Purchased this product a little over a year ago. Feel free to contact me directly for any information I can pass along to you. Heather D. Renko, Histology Supervisor OSF Saint Anthony Medical Center Main Laboratory-Histology 5666 East State Street Rockford, Illinois 61108 815-395-5410 Direct 815-395-5116 Department == The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. == ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] re: incomplete sections
From personal experience some possible solutions: * Infiltration issues ( is your paraffin fresh, carry over on reagents) * Blocks being too warm or too wet * Once had this issue when cleaning of the processor wasn't being properly followed. Hope this helps a bit. Heather D. Renko, Histology Supervisor OSF Saint Anthony Medical Center Main Laboratory-Histology 5666 East State Street Rockford, Illinois 61108 815-395-5410 Direct 815-395-5116 Department == The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. == ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] re: ViAS
Anyone out there using VIAS for Quantitative Analysis of ER/PR? Please email me direct as I have a few questions. Thanks you! Heather D. Renko, Histology Supervisor OSF Saint Anthony Medical Center Main Laboratory-Histology 5666 East State Street Rockford, Illinois 61108 815-395-5410 Direct 815-395-5116 Department == The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. == ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] re: control slide storage
Anyone have any published literature for reference on storage control; refrigerator vs. room temp vs. freezer. I have a friend asking for this and would also would love to get this information for my own reference I searched the archives but, came up with only one publication. Thank you in advance. Heather D. Renko, Histology Supervisor OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 heather.d.re...@osfhealthcare.org == The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. == ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] re: Cut off times for tissue to be processed
I am trying to develop a policy for cut off times for putting tissue into the processor and wanted find out if anyone out there would like to share what they have. Please email me directly-thank you in advance. Heather D. Renko, Histology Supervisor OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 heather.d.re...@osfhealthcare.org == The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. == ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
