Re: [Histonet] IHC validations

[Ingles Claire via Histonet]

Paula:
Usually CLIA follows CAP's lead or has less strict guidelines. Following the 
CAP guidelines should be good for both.
Claire

From: Paula via Histonet 
Sent: Tuesday, June 2, 2020 11:25 AM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] IHC validations

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Hello,

I see 10 positive and 10 negative cases for CAP guidelines, but what about
for CLIA?  What is their guideline to validate an IHC antibody?

Thanks in advance,

Paula

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Re: [Histonet] UV Light in Fume Hood

[Ingles Claire via Histonet]

UV-C wavelength is the best for the virus killing.
Claire

From: Paula via Histonet 
Sent: Thursday, May 14, 2020 1:28 PM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] UV Light in Fume Hood

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Hello everyone.. I hope all is well.



My admin wants me to look into possibly adding a UV light inside our fume
hood, which currently does not have one.  I'm thinking maybe it's not as
effective to kill viruses as we think it would be.



If anyone can share your thoughts about it, or if anyone has bought one and
has any insights to share, that would be greatly appreciated.



Have a great day and take care,

Paula

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Re: [Histonet] guidelines for using a microwave designated for food, but used to heat up histogel

[Ingles Claire via Histonet]

OMG!!! really?!?
OSHA, Joint Commission, CAP, ANY regulatory agency. Give them the lounge 
microwave and get a new one for your break room immediately!! Fumes, 
contamination on the outside of whatever it's in, etc. Not even mentioning if 
it gets spilled.
But then again, our docs like to store Botox in our lunch fridge once in a 
while... *sigh*
Claire


From: Eileen Akemi Allison via Histonet 
Sent: Thursday, December 12, 2019 6:12 AM
To: Histonet 
Subject: [Histonet] guidelines for using a microwave designated for food, but 
used to heat up histogel

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Good morning histopeeps:

We recently brought on an Ophthalmology Pathologist from MD Anderson and they 
use histogel for orienting their thin eye specimens and need a microwave to 
heat it up.  We do not have a microwave in the lab for their use but I had 
ordered one for them.  I just found out they used the microwave in our lab 
lounge to heat up histonet which was opened and being stored in our gross area 
refrigerator.

Do any of you know the CAP regulation against use of laboratory reagents being 
used in a microwave being used for food consumption?  I ended up removing the 
microwave they used and put it in the histology lab.  Our Director wants me to 
write a formal letter to submit to him.

Thank you in advance for your impute!

Akemi Allison, BS,  HT/HTL (ASCP)


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Re: [Histonet] Flammable Sprays in Cryostats

[Ingles Claire via Histonet]

Liquid Nitrogen guns.
Claire

From: Knutson, Deanne via Histonet 
Sent: Wednesday, September 25, 2019 7:50 AM
To: 'histonet@lists.utsouthwestern.edu' 
Subject: [Histonet] Flammable Sprays in Cryostats

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I just received a letter from Leica Biosystems where they are prohibiting the 
usage of flammable
freezing sprays in their cryostats.  What are others using in their cryostats 
to instantly freeze specimens?

Thank you.


Deanne Knutson
Supervisor
Anatomic Pathology
dknut...@primecare.org






  
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Re: [Histonet] Xylene substitutes for clearing agents

[Ingles Claire via Histonet]

Chris:
Propar from Anatech works great for us. I believe it is still advisable to use 
xylene in the cleaning cycle on the processors though. We had to go back the 
other way a bit when our Doc wanted a tape coverslipper. Now he gripes about 
the xylene smell. Hmmm.
Claire


From: Hagon, Christopher (Health) via Histonet 

Sent: Tuesday, August 27, 2019 10:53 PM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Xylene substitutes for clearing agents

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UNOFFICIAL

Hello histonetters!

I realise that this has been asked a lot, but cannot find a good link for the 
comparisons of each. I am charged with looking into converting our lab to go 
xylene free. We don't want to go down the limonene path, so that leaves the 
isopropyl alcohol method, or the aliphatic hydrocarbon xylene substitution 
(Leica Sub-X etc).

Looking for opinions from each camp if possible, on how easy or hard it was to 
change procedures. Was there much trial and error in changing the processing 
protocols with the aliphatics? Any pitfalls I should look out for?

Any input greatly appreciated.


Chris Hagon | Senior Scientist, Anatomical Pathology
ACT Pathology | health.act.gov.au



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Re: [Histonet] Softening Calf Hooves

[Ingles Claire via Histonet]

Sandra:
We here at the Dermatopathology department of
UW Mad have found:
-10% Potassium Hydroxide works best for us on our nails.

-We usually only need to have it in solution for a few hours. As you are trying 
to soften hoof, I might play with both concentration and time in solution.

- I don't believe Monty Python ever actually stated the relative air speed of 
either type of unladen swallow. 


Say HI to Vicki K for me.
Claire Ingles

From: Rene J Buesa via Histonet 
Sent: Thursday, March 21, 2019 12:32 PM
To: Sandra Cheasty; Histonet (histonet@lists.utsouthwestern.edu)
Subject: Re: [Histonet] Softening Calf Hooves

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Either potassium or sodium hydroxide will work at 10% aq. solution. Check it 
daily until soft enough.As to your "swallow" question, I "Goggled" your 
question and the answer is the following"
In the end, it's concluded that the airspeed velocity of a (European) unladen 
swallow is about 24 miles per hour or 11 meters per second. But, the real 
question is not about swallows at all. King Arthur in the movie had two coconut 
shells that he banged together to simulate the sound of a horse galloping.Jul 
7, 2013
René


On Thursday, March 21, 2019 12:06 PM, Sandra Cheasty via Histonet 
 wrote:


 Hello all,
I have 1 inch thick cross sections (band saw) of calf hooves. 
The pathologist has already decaled the bone, and now we have to soften the 
hooves so she can take thinner sections for processing.


*Which is better, potassium hydroxide or sodium hydroxide, and what 
concentration?

*How often should they be checked for softening?

*What is the relative air speed of an un-laden swallow? (European)

Thanks for your help!
Sandy

Sandra J. Cheasty, HT (ASCP)
Histology & Necropsy Supervisor
UW-Madison, School of Veterinary Medicine

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[Histonet] ST4020 Stainer

[Ingles Claire via Histonet]

Netters!
A question on behalf of a co-worker. We are in the process of validating the 
ST4020 stainer from Leica. We are a Mohs clinic that currently uses Carazzi's 
double strength Hematoxylin. We are looking for a better staining protocol. We 
are planning on moving to a Harris formula when switching so any Heme formula 
is possible right now.
Claire
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Re: [Histonet] Processing cystic tissue

[Ingles Claire via Histonet]

Charles:
I usually get out as much of the cystic material as I can without damaging the 
cyst wall itself. That is all the docs are looking at anyway. I am assuming you 
are referring to skin cysts, not ovaries, etc.

Claire


From: Charles Riley via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Tuesday, April 04, 2017 9:05 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Processing cystic tissue

Can anyone give me an idea how they process cystic tissues. THe normal
tissue processes extremely well on my current protocol but the cystic areas
just are too soft to cut. Any tricks anyone can provide to process better
or cut better after processing would be greatly appreciated

--

Charles Riley HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
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Re: [Histonet] Reference for this Recipe and Use

[Ingles Claire via Histonet]

Sadly, it is much safer than the old Chloroform clearing method.
Claire


From: Tony Henwood (SCHN) via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Sunday, March 26, 2017 6:22 PM
To: ian bernard
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Reference for this Recipe and Use

This is sometimes known as Newel's solution for revealing lymph nodes in fatty 
specimens:

Newell KJ, Sawka BW, Rudrick BF, Driman DK. (2001) "GEWF solution" Arch Pathol 
Lab Med 125:642 645.

Absolute ethanol1000ml
Water   340ml
Concentrated formalin (37%) 160ml
Glacial acetic acid 100ml



Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA

-Original Message-
From: ian bernard via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Saturday, 25 March 2017 3:04 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Reference for this Recipe and Use

500 ML OF 100% ALCOHOL

170 ML OF DISTILLED WATER

80 ML OF 40% FORMALIN

50 ML OF GLACIAL ACETIC ACID



V/R

IB



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Re: [Histonet] Performing PAS with Diastase by Hand

[Ingles Claire via Histonet]

We get our Diastase powder from American Mastertech.

Claire
Dermatopathology lab
University of Wisconsin Hospital and Clinics


From: Vickroy, James via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Wednesday, October 05, 2016 3:48 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Performing PAS with Diastase by Hand

We are starting to perform a PAS with diastase for fungus to be used by our 
dermatopathologist.   At first we weren't sure he was going to need a method 
that used diastase but he now prefers it.  Problem is our PAS counter kit did 
not come with diastase.  Please don't tell me to use saliva although I'm old 
enough to know we did for years.  We did order some powered diastase that was 
quite expensive and of course the working solution had a very short shelf-life. 
  Can anybody recommend a product and vendor that is relatively inexpensive and 
economical to use?

Jim

Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvick...@springfieldclinic.com



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Re: [Histonet] Histonet Digest, Vol 151, Issue 1

[Ingles Claire via Histonet]

Not to mention that if you inhale too many fumes you will pass out. One of the 
original anesthetics. :)
Claire


From: Douglas Gregg via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Wednesday, June 01, 2016 1:04 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Histonet Digest, Vol 151, Issue 1

Subject: Re: [Histonet] Histology tips

Here is one that your safety officer will like. Ether is sometimes called
for as a de-stainer but is dangerous to keep except in a sealed can. Once
opened, it can be quite explosive in a frig. This is my solution. Get a
cheap can of engine starting fluid. It is pure ether. Don't get the high
priced version with lubricants added. You just use what you need from the
spray can and the rest keeps just fine for years.

Douglas Gregg DVM PhD
Veterinary pathologist
Southold, NY
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Re: [Histonet] PAS Stain

[Ingles Claire via Histonet]

I don't know, I believe Dr. Salk did the same with the polio vaccine. He even 
involved his family! Dedicated doctors...
Claire


From: Tony Henwood (SCHN) via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Thursday, May 05, 2016 6:33 PM
To: Anne Murvosh
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] PAS Stain

Only a crazy Aussie would do this!!

Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA


-Original Message-
From: Anne Murvosh via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, 6 May 2016 6:03 AM
To: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] PAS Stain

You clearly don't know your histo history. The reason we know that H pylori 
exists is because a Scientist, Dr. Barry Marshall wanted to prove bacteria 
caused ulcers and not stress.  No one believed him.  So he took the organisms 
from a patient, mixed it in a broth and drank it. He then biopsied himself and 
treated it. There's a non-uniform method that saved a lot of suffering.  Bravo 
we crazy scientists.  Anne


-Original Message-
From: Geoff via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, May 05, 2016 12:31 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] PAS Stain

I cannot believe any scientist would advocate such a non-uniform method as 
spitting on a slide.
Buy a bottle of what ever enzyme and use a reproducible buffer and temperature.

Geoff

On 5/5/2016 3:19 PM, Anne Murvosh via Histonet wrote:
> Yes, spitting is the tried and true way to do it.  Not to mention no 
> measuring and cheaper.  The reason we switched to a powder is because I just 
> don't spit well I used to have someone do it for me cause I would end up 
> drooling. YUCK! The best way to find out is do the amylase method and the 
> spit method at the same time and have the doctor pick the best.  A fun 
> experiment  Anne
>
> -Original Message-
> From: Bob Richmond via Histonet
> [mailto:histonet@lists.utsouthwestern.edu]
> Sent: Thursday, May 05, 2016 11:36 AM
> To: koelli...@comcast.net
> Cc: Histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] PAS Stain
>
> Spokane Ray points out something I've wondered about for years - can
> just anybody spit on the slide and remove the glycogen? I've never
> heard of any variation, but the number of people I've asked is very
> limited. This
> reference:
> http://www.ncbi.nlm.nih.gov/gene/276
> certainly suggests that different people have different salivary alpha
> amylase activity.
>
> Bob Richmond
>
> On Thu, May 5, 2016 at 2:27 PM,  wrote:
>
>> I love having the Samuri Pathologist on this forum for wisdom and
>> real-laboratory life knowledge.  And yes, I have in the past spit on
>> slide ON OCCASSION when faced with a dire necessity.  Although I know
>> there are those who would wretch about this; it remains a fact of
>> viable laboratory life for some.
>>
>> My problem now is that in this era of (MUCH TOO MUCH) regulation, how
>> do you "test lots" or control from "lot-to-lot variation" in this
>> SOP?  When Jane or Joe do this routinely and then goes on vacation,
>> what about Sally or Jim spit?  There is a variation in copy number of
>> the AMY1 gene
>> (amylase) and resulting difference in amylase protein concentration
>> amongst individuals.
>>
>> Why not just standardize it from the start, reagent, pH, temperature
>> and it really cannot fail.
>>
>> Spokane Ray
>>
>> --
>> *From: *"Bob Richmond via Histonet"
>> 
>> *To: *"Histonet@lists.utsouthwestern.edu" <
>> histonet@lists.utsouthwestern.edu>
>> *Sent: *Thursday, May 5, 2016 11:10:40 AM
>> *Subject: *Re: [Histonet] PAS Stain
>>
>>
>> Amylase (diastase) for the PAS stain queries:
>>
>> Whatever happened to spitting on the slide (30 min at room temperature)?
>> John Kiernan advises "thinking of lemons and drooling into a small beaker"
>> though I'd advise chewing on a rubber band for a few seconds.
>>
>> He notes that alpha amylase is preferred. I'd go with the cheapest
>> one in the Sigma-Aldrich catalog. Room temperature is usual, but I
>> note that Sigma offers a heat-stable alpha amylase.
>>
>> Bob Richmond
>> Samurai Pathologist
>> Maryville TN
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Re: [Histonet] PAS Stain

[Ingles Claire via Histonet]

The level of amylase in your saliva also depends on when you ate last. If 
you're spitting on slides after you just ate, the reaction will be weaker as 
the amylase will have been used up on lunch digestion. (also, you need to be 
careful about having your lunch salad contaminate the slide... :)
Claire


From: Ray via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Thursday, May 05, 2016 1:48 PM
To: Richmond, Bob
Cc: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] PAS Stain

An excellent point.  For anyone wanting to investigate-simply do a PubMed 
search on variation of AMY1 gene.  Sorry; I guess I should say this is, 
strictly speaking, non-histology related topic and I don't want to get into 
trouble as some before me.  Tons of research about this linking back (in 
theory) to positive selection in hunter-gatherers versus agricultural 
ancestors, race, obesity, phenotypic and dietary differences as to why maybe 
there can be big differences.
Spokane Ray

- Original Message -

From: "Bob Richmond" 
To: koelli...@comcast.net
Cc: "Histonet@lists.utsouthwestern.edu" 
Sent: Thursday, May 5, 2016 11:35:42 AM
Subject: Re: [Histonet] PAS Stain

Spokane Ray points out something I've wondered about for years - can just 
anybody spit on the slide and remove the glycogen? I've never heard of any 
variation, but the number of people I've asked is very limited. This reference:
http://www.ncbi.nlm.nih.gov/gene/276
certainly suggests that different people have different salivary alpha amylase 
activity.

Bob Richmond

On Thu, May 5, 2016 at 2:27 PM, < koelli...@comcast.net > wrote:



I love having the Samuri Pathologist on this forum for wisdom and 
real-laboratory life knowledge.  And yes, I have in the past spit on slide ON 
OCCASSION when faced with a dire necessity.  Although I know there are those 
who would wretch about this; it remains a fact of viable laboratory life for 
some.

My problem now is that in this era of (MUCH TOO MUCH) regulation, how do you 
"test lots" or control from "lot-to-lot variation" in this SOP?  When Jane or 
Joe do this routinely and then goes on vacation, what about Sally or Jim spit?  
There is a variation in copy number of the AMY1 gene (amylase) and resulting 
difference in amylase protein concentration amongst individuals.

Why not just standardize it from the start, reagent, pH, temperature and it 
really cannot fail.

Spokane Ray


From: "Bob Richmond via Histonet" < histonet@lists.utsouthwestern.edu >
To: " Histonet@lists.utsouthwestern.edu " < histonet@lists.utsouthwestern.edu >
Sent: Thursday, May 5, 2016 11:10:40 AM
Subject: Re: [Histonet] PAS Stain


Amylase (diastase) for the PAS stain queries:

Whatever happened to spitting on the slide (30 min at room temperature)?
John Kiernan advises "thinking of lemons and drooling into a small beaker"
though I'd advise chewing on a rubber band for a few seconds.

He notes that alpha amylase is preferred. I'd go with the cheapest one in
the Sigma-Aldrich catalog. Room temperature is usual, but I note that Sigma
offers a heat-stable alpha amylase.

Bob Richmond
Samurai Pathologist
Maryville TN
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Re: [Histonet] Running a histo lab without a histotech?

[Ingles Claire via Histonet]

Not to my knowledge. We have 3 derm/Mohs labs here in our system. There are 
only two of us who have our certifications. Heaven knows the world (or the lab 
for that matter) doesn't stop revolving when WE go on vacation. The third lab 
is a one person show and she isn't certified. Just so all the CLIA/JCHO/CAP 
stuff is being adhered to. The only thing I can think of regarding 
training/college must haves are for grossing as it is considered high 
complexity. I agree with Rene. Not your problem.
Claire


From: Rene J Buesa via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Friday, October 23, 2015 7:35 AM
To: Jay Lundgren; histonet
Subject: Re: [Histonet] Running a histo lab without a histotech?

About the "legality" I do not think it is of your direct concern, unless you 
want to "challenge" the situation with some sort of legal action, which is 
really not advisable.As a functioning laboratory in a hospital it has to be 
under the supervision of either CLIA or  ASAP and the pathologist is the 
Director of such laboratory so, it is the pathologist's responsibility of 
assuming about the "legality" of that situation and is the pathology who you 
should ask this question.René


 On Thursday, October 22, 2015 5:02 PM, Jay Lundgren via Histonet 
 wrote:


 Hello histonetters!  I am on assignment in a small hospital lab in the
Heartland and they are staffed by only two people.  One is a registered
(ASCP) HT with 10 years of experience, and the other is a former
phlebotomist being trained on the job from scratch , who has yet to take
her accreditation exam.

 As a matter of fact, she has yet to take all the college courses (math,
science) to qualify to take her exam, and is still being  trained in the
hospital's protocols, only allowed to cut larger specimens (no bxs), etc.
She can accession, answer the phone, embed, load the stainer, coverslipper,
etc.,  so she can turn out most of the work.  I am here working for three
weeks because the trainee is on medical leave.

My question is, when the registered HT goes on vacation or sick leave, is
it legal for the lab to operate with only the trainee?

Or does a histo lab have to have at least one registered tech to operate?


  Thanks,

  Jay A. Lundgren, M.S., HTL (ASCP)
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Re: [Histonet] are you desperate enough to hire a B.Sc. graduate?

[Ingles Claire via Histonet]

We have had this problem for years. We are more than willing to train someone. 
We are a small lab so a bigger concern is if they will fit in with the rest of 
us personality wise. Not that we just hire anyone willy-nilly, but many people 
with BS's have some lab experience/background anyway. We just make sure they 
have at least a BS so they fit the qualifications to gross. I am actually the 
only one in a lab of 4 with my certification, and though I also have an AA in 
Histotechnology, my BS in Biology made getting the AA alot easier. It is more 
of a concern if they can't troubleshoot and adapt on the fly to the ever 
changing situations in the lab. We actually just hired an HT with 28 years of 
experience, but don't know if she is going to make it past her 6 month eval. 
She can't multitask and has a hard time adapting to the different specimen 
types. I think she has worked mostly in research in the past. (not belittling 
you researchers out there, but many of you know that research vs. cli
 nical work can take different skill sets.)
Claire


From: Michael LaFriniere via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Monday, August 10, 2015 1:39 PM
To: tgen...@gmail.com
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] are you desperate enough to hire a B.Sc. graduate?

Yes this is a common problem, although I am not sure desperate enough would 
be appropriate  verbiage.  As histologists over the years we have learned to 
cope with and being highly resourceful. I have also resorted to the same idea, 
hiring both AS and BS individuals filling positions and training specific 
technical task to meet the production needs at present has worked very well in 
my laboratory.

Michael
Michael R. LaFriniere, HT (ASCP)
Executive Director


Capital Choice Pathology Laboratory
12041 Bournefield Way, Suite A . Silver Spring, MD 20904
P: 240.471.3427 . F: 240.471.3401 . Cell 410-940-8844
michael.lafrini...@ccplab.com



-Original Message-
From: Tyrone Genade via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, August 10, 2015 12:13 PM
To: histonet
Subject: [Histonet] are you desperate enough to hire a B.Sc. graduate?

Hello,

Last week a I visited Pittsburgh and had a chance to talk with a fellow 
histologist there. He remarked on the dire state his lab is in with respect to 
finding qualified histologists to employ; and that the lab was now considering 
hiring B.Sc. graduates and training them up in sectioning etc... Is this a 
common problem, the trouble finding qualified histotechs, and are other labs 
also considering hiring B.Sc. graduates to staff their labs?

I have one student working with me (with medical school aspirations) who 
sections beautifully. There is surely talent out there...

Bye
--
Tyrone Genade
Orange City, Iowa
tel: (+1) 712 230 4101
http://tgenade.freeshell.org

Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord.
To find out how to receive this FREE gift visit http://www.alpha.org.
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Re: [Histonet] Manual Cassette Labelling

[Ingles Claire via Histonet]

We use Secureline Marker II/superfrost Histoprep pens from Fisher. Their item # 
is 14-905-30 for black, 14-905-35 for red. We have never had a problem with 
them smearing or fading on the cassettes no matter what we subject them to.
Claire


From: Joanne Clark via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Thursday, August 06, 2015 3:27 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Manual Cassette Labelling

Hi Histoland,

We have a satellite lab that manually prepares their cassettes.  We have had 
significant problems with the markers coming off after tissue processing.  We 
have tried StatLab markers, Leica Markers and the red AquaRellable pencils and 
continue to have issues.  Other than purchasing a cassette labeling system 
(their volumes are pretty low and there is limited bench space in the lab for a 
cassette labeler) does anyone have any suggestions or ideas on what might be 
causing this phenomenon or a really good permanent lab marker that has been 
successful for you?

Joanne Clark, BAAS, HT(ASCP)CM
Director of Histology

P.   (575) 622-5600
C.   (575) 317-6403
F.   (575) 622-3720
TF. (800) 753-7284

pcnm.com




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Re: [Histonet] Marciafava-Bignami Disease

[Ingles Claire via Histonet]

I know I watch my exposure to Formalin and Xylene especially. I was diagnosed 
with NASH in 2007 (who knows how long before then, since I am asymptomatic) 
when I had a Pituitary Adenoma that had hemorraged (Boy talk about a headache!) 
Anyway, I have always wondered about the cause. I thought it was genetic as my 
father also had it for over 40 years before finally having a transplant. But I 
wonder if it was his exposure to jet fuel, etc. as he was a jet mechanic for 20 
years in the USAF plus another 15-20 with a private company. So far my liver 
has been stable even getting a bit better to the point of being just a hair 
above the normal range. I do everything under the sun in my lab. Mohs, 
Grossing, plus all the routine stuff of cutting and staining, etc. I have been 
able so far to keep the repetitive injuries at bay, and Dansco shoes have saved 
my feet from the Plantar Faciitis (had that about a year straight at one 
point.). We had managed to keep xylene out of our lab for years by usi
 ng Propar, but one of the Pathologists insisted on having a tape coverslipper. 
We still use it as little as possible. I had a co-worker, when she was pregnant 
wasn't allowed to gross (hence contact with formalin). Because of the NASH I 
get blood tests done every 6 months. It will be interesting to see if they have 
changed possibly due to the exposure to xylene, which I haven't had exposure to 
for years otherwise.
Claire


From: Mayer,Toysha N via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Tuesday, August 04, 2015 2:49 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] Marciafava-Bignami Disease

This is very interesting.  I have worked in the field for over 20 yrs, and was 
pregnant while working in a small lab.  Mostly everything was manual, and I did 
not use all of the safety precautions I should have (my fault). My son had 
severe speech and language delay as well as a language processing issue when he 
was smaller, and now stutters.  I have often wondered (as most parents would) 
if my lack of precautions may have contributed. We stress the safety issues to 
the students daily, to get it all in their heads.  I often tell  people that I 
am so happy because I work with xylene, and I feel real good.  So far, I don't 
see any noticeable symptoms of anything wrong, just the usual-decreased 
olfactory sensing, repetitive motion issues, plantar fasciitis, and varicose 
veins.  Hair loss was an issue at one time, but that was attributed to PCOS.  
Now that I am not in the lab full time anymore it has decreased since I went 
natural (I'm African-american).  One thing I have noticed is my C
 -reactive protein is high, and I take a statin for that.  I have no liver 
issues, and my functions appear to be normal.  Next time I go in for a 
physical, I will have my workplace hazards documented in my EMR.
I will look up Primary Biliary Cirrhosis (PBC), to get some more information, 
especially since I was born jaundiced and I am anemic.


Sincerely,

Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP)
Instructor/Education Coordinator
Program in Histotechnology
School of Health Professions
UT M.D. Anderson Cancer Center
713.563-3481


Message: 11
Date: Tue, 4 Aug 2015 06:30:12 -0700
From: Eileen Akemi Allison akemiat3...@gmail.com
To: Edmondson David (RBV) NHS Christie Tr
david.edmond...@christie.nhs.uk
Cc: Histonet histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Marciafava-Bignami Disease I have re-logged
into thesystem and maybe this will now communicate with 
Histologists
out there,  and hope that I do not get twice as many emails from
Histonet#
Message-ID: e3d7c5a9-c516-4f0e-b522-61b348820...@gmail.com
Content-Type: text/plain;   charset=utf-8

GP?s are fine for general health issues, but I would certainly get more 
conclusive tests done by a neurologist, as well as contacting the best worker?s 
comp attorney in your area who has dealt with chemical exposure cases.

Studies show we are #1 in this country for the most hazardous professions. It's 
safe compared to when I 1st started in this field in 1965! Well, I am a 
dinosaur from back in the day when we had inadequate ventilation, a shortage of 
fume hoods, inadequate education on hazards, safety and PPE's.

In 1979 I started to work at OHSU in the surgical path lab, as well as doing 
research projects. We made up all of our own HE's and special stains from 
scratch, as well as made up our own 10% NBF in 55 gallon drums without fume 
hoods, ventilation, masks or gloves. I also worked with Glyco-Methacrylate 
embedded tissues without hoods or gloves! Since it was a medical school, we did 
every special stain under the sun and dealt with about every chemical, reagent, 
acid and stain you could think of! We also smoked cigarettes and drank coffee 
in the lab while we embedded and cut! We sure were a naive group back then!

In the early days, the facilities I 

[Histonet] RE: Paraffin-Plastic Stratification/Congo red problem

[Ingles Claire]

I believe amyloid does fade with age on cut slides. Frieda sited Bancroft and 
Cook that intensity decreases with age and long-standing deposits stain less 
intensely than smaller Newly formed deposits. She also lists a Crystal Violet 
method in the 3rd edition, but that it is more of a rapid screening technique 
and is not as specific as Congo Red. 
Claire


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Wheelock, Timothy R. 
[twheel...@mclean.harvard.edu]
Sent: Friday, January 23, 2015 2:00 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Paraffin-Plastic Stratification/Congo red problem

Hi Jeffery:

Yes, I notice the same paraffin dust bunnies, perhaps especially since I use 
a embedding paraffin that has a fair amount of plastic.
Before I embed my brain tissue, I mix the embedding media thoroughly, until the 
solution is clear.

As for your Congo Red problem, I wish that I could help you.
I am now experiencing the same problem, except that it affects the positive 
controls as well.
The staining is there, but much fainter than it should be.

I usually immerse albumin coated slides in 1% Congo red for 15 minutes, 
differentiate them with Potassium Hydroxide for 3 dips, counterstain with 
Harris Hematoxylin for 1 minute, differentiate with acid alcohol for 5-10 dips, 
immerse in running tap water for 10 minutes, immerse in 95% ethanol for 2 dips, 
then dehydrate and mount.

Tim




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[Histonet] Sakura slide racks

[Ingles Claire]

Histonetters!!

I am looking for some replacement racks that hold 20 slides and that fit in the 
Sakura Tissue-Tek film coverslipper. For some reason our ordering department 
doesn't want us to/let us order directly from Sakura.

Thanks

Claire

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[Histonet] Heme boogers

[Ingles Claire]

Hello All!



We have been having an interesting problem with our Hematoxylin. It seems there 
are globules that form on the bottom of our hematoxylin wells in our automatic 
stainer (Leica XL). These globs are acellular, so it is not a fungus or 
something. We use a progressive Mayer's Hematoxylin formula as follows:



1000ml DI

50g Aluminum Ammonium Sulfate

1g Hematoxylin powder

1g Citric Acid

0.2g Sodium Iodate



Anyone know what this might be? It doesn't affect the staining, but likes to 
hang on the bottom of the staining racks and is a bit gross. Plus it gets 
dragged into some of the following wells on occasion.



Thanks!

Claire

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RE: [Histonet] Re: Flames at embedding centers

[Ingles Claire]

Old Histotechs never die. They're just well fixed... :).


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Mark Turner 
[mtur...@csilaboratories.com]
Sent: Wednesday, November 19, 2014 1:53 PM
To: Sanders, Jeanine (CDC/OID/NCEZID); Blazek, Linda; 
Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Flames at embedding centers

We had a special clean counter we used for pizza on a  regular basis.

I worked with a pathologist who refused to wear gloves and would gross colons 
bare-handed.  Guy is still alive and kicking at 85!

In the very old days, we used carbon tetrachloride to dehydrate in the open 
tissue processor (Technicon).  Not going to say anything at all about 
disposal

Mark Turner,  Ph.D., HT(ASCP)QIHC
Manager, Histology/IHC


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, 
Jeanine (CDC/OID/NCEZID)
Sent: Wednesday, November 19, 2014 2:10 PM
To: Blazek, Linda; Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Flames at embedding centers

Okay, since we are confessing.
When I was a student in histology school, we did the potluck thing IN THE LAB! 
I mean, ALL the food was laid out on a back 
counter

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda
Sent: Wednesday, November 19, 2014 2:08 PM
To: Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Flames at embedding centers

Since the break room was right across the hall from the histology lab, we use 
to clear off a counter to put all the food for our potlucks.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, 
Jeanine (CDC/OID/NCEZID)
Sent: Wednesday, November 19, 2014 2:04 PM
To: James Watson; 'Bob Richmond'; Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Flames at embedding centers

sigh the good old days

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of James Watson
Sent: Wednesday, November 19, 2014 1:58 PM
To: 'Bob Richmond'; Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Flames at embedding centers

How about the person in the 1970's coverslipping with an open dish of xylene at 
AFIP and someone at the other end of the stain line decolorizing Brown and 
Brenn stains with acetone/ether in the sink; then the acetone/ether fumes 
migrating across the stain line to the cigarette and having the whole counter 
and wall catch fire.

James Watson HT  ASCP
GNF  Genomics Institute of the Novartis Research Foundation Scientific 
Technical Leader II, Histology Tel858-332-4647 Fax   858-812-1915 
jwat...@gnf.org

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond
Sent: Wednesday, November 19, 2014 10:36 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Flames at embedding centers

Remembering histotechnologists at Johns Hopkins in the 1960s smoking cigarettes 
while hand-staining slides in rows of large Stender dishes, including a dish 
with 20% picric acid in acetone, used to remove formalin pigment (since 
buffering formalin wasn't permitted way back then).

Bob Richmond
Samurai Pathologist
Maryville TN
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RE: [Histonet] On the lighter side...

[Ingles Claire]

Old histologists never die, they are just well fixed...
Claire

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Michael Ann Jones 
[mjo...@metropath.com]
Sent: Monday, August 11, 2014 9:07 AM
To: Edwards, Richard; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] On the lighter side...

25 years, (what¹s that in micron¹s?²) Bernice, you are too funny!!
(lots of tenure here . . .lotsa brain cells)
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 8/11/14, 5:17 AM, Edwards, Richard r...@leicester.ac.uk wrote:

Sniffed my  first formalin and  saw  first post-mortem November 1965.

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RE: [Histonet] On the lighter side...

[Ingles Claire]

Just a foot note to all this. I still can't get my certification listed on my 
work badge because they've never heard of it. I'll just have to give them a 
tutorial...*sigh*  I think this type of stuff is what gets me burned out the 
easiest. The thing that saves me are my co-workers. Tim is right about the 
meetings though. Besides you can also learn little tricks from the More 
Experienced among us. Teach me O Wise Ones. I am but forever a novice. :)
Claire


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Morken, Timothy 
[timothy.mor...@ucsfmedctr.org]
Sent: Friday, August 08, 2014 11:45 AM
To: 'Beth Brinegar'; Elizabeth Chlipala
Cc: histonet@lists.utsouthwestern.edu; Cartun, Richard
Subject: RE: [Histonet] On the lighter side...

Yah! A new one!!! Stay on Histonet, go to meetings and stay interested- the 
burnout prevention!

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA
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RE: [Histonet] On the lighter side...

[Ingles Claire]

15 years and over 10 certified HTL. 
Claire Ingles

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Richard A. Carter 
[rcar...@cancer-test.com]
Sent: Thursday, August 07, 2014 4:36 PM
To: 'Jennifer MacDonald'; Douglas Porter
Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] On the lighter side...

28 years

Rick Carter

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer 
MacDonald
Sent: Thursday, August 7, 2014 2:20 PM
To: Douglas Porter
Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu
Subject: Re: [Histonet] On the lighter side...

31 years

Jennifer MacDonald



From:   Douglas Porter doug.por...@caplab.org
To: histonet@lists.utsouthwestern.edu
Date:   08/07/2014 11:36 AM
Subject:[Histonet] On the lighter side...
Sent by:histonet-boun...@lists.utsouthwestern.edu



How long have you been a registered histotech?  36 years here.  You???



Douglas A. Porter, HT (ASCP)
Grossing Technician
IT Coordinator

Cancer Registrar


CAP-Lab, PLC
2508 South Cedar Street
Lansing, MI 48910-3138

517-372-5520 (phone)
517-372-5540 (fax)

 mailto:doug.por...@caplab.org doug.por...@caplab.org

 http://www.caplab.org/ www.caplab.org



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RE: [Histonet] Basement Lab

[Ingles Claire]

Our main Histology lab at UW Madison Hospital is in the basement of the VA 
(They're) attached. We have to go UP a floor to get to the morgue. Although why 
they put the morgue on the main level is beyond me... Oh, wait. government 
administration... I am at one of the outlying clinics and at least we can walk 
by windows once in a while. :)  But for some reason we do get paid more than 
the med techs here. Knock me over with a feather. 
Claire


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[Histonet] RE: Req Storage

[Ingles Claire]

I'm guessing the procedure you discribe does, as we have been doing it that way 
for years. We keep our hard copies on site for a week before sending them to be 
scanned.
Claire

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Nails, Felton 
[flna...@texaschildrens.org]
Sent: Tuesday, March 25, 2014 10:11 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Req Storage

Has anyone started scanning requisitions and discarding the original AP reqs?
If so does this meet the CAP retention requirement?





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[Histonet] OT: RE: wax on the floors

[Ingles Claire]

When you're finished with him, send Bruce my way!!!
Claire


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Blazek, Linda 
[lbla...@digestivespecialists.com]
Sent: Wednesday, December 11, 2013 2:32 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: wax on the floors

I'm still looking for Bruce the stripper!
We use to use a paraffin floor scraper kind of like a scraper on the end of a 
broom stick but somehow it disappeared.  We now use a paraffin scrapper that is 
much easier on the floor.  The one designed for scrapping the floor had a blade 
that had a tendency to gouge the floor.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mayer,Toysha N
Sent: Wednesday, December 11, 2013 3:17 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Re: wax on the floors

Ok, I could not resist.  Let's see, I have used:
Paint scraper
Slide (when I first started)
Spackle spatula taped to a broomstick (when I worked at small, poor place) Ice 
thingy (removes the ice from car windshield)  hey I live in Houston, we don't 
have ice Floor scraper from a carpet/vinyl floor company (when I worked in a 
fancy lab)

Also, don't let housekeeping wax the floors.



Sincerely,

Toysha N. Mayer, MBA, HT(ASCP)
tnma...@mdanderson.org
Instructor/Education Coordinator
Program in Histotechnology
School of Health Professions
MD Anderson Cancer Center
713-563.3481


Message: 1
Date: Wed, 11 Dec 2013 09:49:03 -0800 (PST)
From: Paula Pierce cont...@excaliburpathology.com
Subject: Re: [Histonet] wax on the floors
To: Victor A. Tobias vtob...@uw.edu,Histonet
histonet@lists.utsouthwestern.edu
Message-ID:
1386784143.76836.yahoomail...@web5706.biz.mail.ne1.yahoo.com
Content-Type: text/plain; charset=iso-8859-1

A brand name! LOL
?
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
5830 N Blue Lake Dr. Please note new address!
Norman, OK 73069
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com



 From: Victor A. Tobias vtob...@uw.edu
To: Paula Pierce cont...@excaliburpathology.com; Nancy Schmitt 
nancy_schm...@pa-ucl.com; Histonet histonet@lists.utsouthwestern.edu
Sent: Wednesday, December 11, 2013 11:25 AM
Subject: RE: [Histonet] wax on the floors


Is Bruce a Brand name or the employee?

Victor Tobias HT(ASCP)
Clinical Applications Analyst
Harborview Medical Center
Dept of Pathology Room NJB 244
Ninth  Jefferson
Seattle, WA 98104
vtob...@u.washington.edu
206-744-2735
206-744-8240 Fax
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce
Sent: Wednesday, December 11, 2013 9:17 AM
To: Nancy Schmitt; Histonet
Subject: Re: [Histonet] wax on the floors

Bruce floor stripper!
?
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
5830 N Blue Lake Dr. Please note new address!
Norman, OK 73069
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com



From: Nancy Schmitt nancy_schm...@pa-ucl.com
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Sent: Wednesday, December 11, 2013 11:05 AM
Subject: [Histonet] wax on the floors


Happy Hump Day!

What is everyone doing to clean floors of paraffin?? Does anyone have any great 
secrets to how the floors are cleaned of the debris?

Thanks for any input!

Nancy

Nancy Schmitt HT, MLT(ASCP)
Histology Coordinator
United Clinical Laboratories




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End of Histonet Digest, Vol 121, Issue 15
*

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[Histonet] RE: Yahoo link

[Ingles Claire]

Old Histologists never die, they're just well fixed...
Claire

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Morken, Timothy 
[timothy.mor...@ucsfmedctr.org]
Sent: Tuesday, December 03, 2013 11:22 AM
To: 'Shirley A. Powell'; Histonet
Subject: [Histonet] RE: Yahoo link

Well, Shirley, you are actually an Angel, so nothing will ever stop you!! (from 
an old Georgia Society hand).

Tim Morken
Supervisor, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shirley A. 
Powell
Sent: Tuesday, December 03, 2013 9:18 AM
To: Histonet
Subject: [Histonet] Yahoo link

When I entered the profession I was told the average life expectancy of a 
histotechs was 20 years from hiring.  That scared me but I was already hooked.  
I have been doing this 51 years, so maybe good laboratory practices can help, 
in spite of bad ventilation and all those other dangers mentioned.

Shirley Powell
Antique Histotech

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce
Sent: Tuesday, December 03, 2013 11:40 AM
To: Morken, Timothy; Histonet
Subject: Re: [Histonet] RE: Yahoo link

Just what I was thinking. We will never get enough new students now!

With proper training and equipment, we do the job safely!




And you can drown in an inch of water. Is that in the MSDS!

Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
5830 N Blue Lake Dr. Please note new address!
Norman, OK 73069
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com



 From: Morken, Timothy timothy.mor...@ucsfmedctr.org
To: 'Mike Tighe' mti...@trudeauinstitute.org; 
histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) 
histonet@lists.utsouthwestern.edu
Sent: Tuesday, December 3, 2013 10:24 AM
Subject: [Histonet] RE: Yahoo link


Great. Just what we need.


Even so, we in the business can take these lists with a grain of K4[Fe(CN) 6] * 
3H 2 O since we know that suitable precautions preclude most of the danger. For 
instance, I'll have to say that the histo lab here is wonderful in that it has 
such good ventilation (ie.,  extraction) that there is none of the usual 
chemical smell - no xylene, alcohol, specials chemicals that often assaults the 
senses in histology. Vendors that come here are amazed.

Tim Morken
Supervisor, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Tighe
Sent: Tuesday, December 03, 2013 6:31 AM
To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] Yahoo link

Anybody wonder who has the most harzardous job to your health? We're Number 
One!!!



http://finance.yahoo.com/news/the-15-jobs-that-are-most-damaging-to-your-health-155706120.html



Mike
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RE: [Histonet] slide and block discards

[Ingles Claire]

I'd be interested in it too. 
Claire


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Cheryl 
[tkngfl...@yahoo.com]
Sent: Monday, February 18, 2013 12:25 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] slide and block discards

Hello All-

Does anyone have some authoritative document stating that histologic slides 
and/or blocks are non-infectious?  We all know this to be true--but is there a 
piece of paper that states this unequivocally?

Thanks!


Cheryl Kerry, HT(ASCP)
Full Staff Inc.
Staffing the AP Lab by helping one GREAT Tech at a time.
281.852.9457 Office
800.756.3309 Phone  Fax
ad...@fullstaff.org

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[Histonet] RE: Something different!

[Ingles Claire]

Seriously cool! Will have to try to read the whole thing in my spare time. The 
first few pages look intriguing already.
Claire
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RE: [Histonet] Basis for Quality Work in a Histotech

[Ingles Claire]

You're right Tim. I don't know how much time I have saved by listening to my 
inner voice and doublechecking things when the case number and name on a 
recut/stain request. One of our docs is famous for putting down wrong case #'s. 
Even when everything looks OK, sometimes the docs accidentally mark the wrong 
stain as our sheet is in a grid and they are mentally in the wrong column. I 
even get suspicious if they order an unusual stain that is sitting right next 
to a more routine on the list. I call it my Histo sense :) 5 minutes of 
double-checking is much more efficient than having to recut and restain again.
Claire


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Morken, Timothy 
[timothy.mor...@ucsfmedctr.org]
Sent: Monday, December 17, 2012 11:52 AM
To: Lynette Pavelich; Sheila Haas; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Basis for Quality Work in a Histotech

Here is our slide labeling procedure. It is simple, but we insist it be 
followed. This will  be modified once barcoding is instituted to use scanning 
ID of slides vs block.
We also instituted block ID beads at the embedding center. That has helped 
tremendously to identify people that may need embedding retraining.


One other thing I should mention. People in the lab should be told to trust 
their inner voice. We had several labeling errors that on investigation were 
suspected or ignored by a tech in the chain or events. In  one case the 
sectioning tech pulled the wrong block from the file, labeled the slides with 
that block number and sent it to the IHC group. An IHC tech took the slide and 
noticed the number was wrong but ASSUMED the sectioning tech had made an error, 
so corrected the number to match the request on her log. The mistake was only 
caught by the pathologist who noticed the IHC slide did not match the HE slide 
from the proper block.

I tell  my techs: Trust your instincts. If something seems wrong - for whatever 
reason - STOP!!! Investigate the issue. You will save time, effort, and maybe 
lives.

I also tell my techs not no pathologist will remember how fast you were, just 
how many mistakes you made.


Tim Morken

+++

Slide Labeling Procedure at the Microtome

Slide labeling at the cutting station is the most important part of the process 
used to identify slides. A mistake at this point will cause a mix-up in slides 
or cases and could lead to improper diagnosis and treatment for patients.

Because this is such an important step the following procedure must be followed 
exactly. Deviation may cause mislabeling. Deviation from the procedure may be 
cause for reprimand and termination.

Important points:
1.  Label slides for one block at a time.
2.  Do not pre-label slides before cutting sections from a block, or for 
any other blocks.
3.  Work with one block at a time as much as possible. If the block must be 
re-soaked before completing the slide count then the block must be isolated 
from the other blocks to prevent a mix-up.
4.  The stainer tech will compare stained slides to the blocks to ensure 
they are correct.


Sectioning Procedure
1)  Pick up the next page of the processing log and the blocks that are on 
that page.
2)  Verify that the blocks picked up are on the processing log.
a.  Put the blocks in order according to the processing log.
b.  Put a check mark “” next to the block line on the log to indicate the 
block is present in the group.
c.  Note any problems with the blocks
1.  Put an “X”  mark next to any problem blocks and note the problem.
a.  Add-ons
b.  Missing
c.  Reprocessing needed
d.  Re-embedding and reason
e.  other
3)  Face-trim the blocks and put them on ice (if necessary) in order as 
shown on the processing log.
4)  Pick up the first block on the log.
a.  Do not pre-label slides for this or any other block
5)  Place the block in the microtome and cut sections according to
a.  requests on the processing log and established sectioning requirements
b.   “Tissue Sectioning Standards” of the Sectioning section of the 
Histology Laboratory manual
6)  Pick up the proper number of sections on the appropriate slides.
7)  Label the slides
a.  Write the Accession number and Part letter/number of the block on the 
microtome.
b.  Label slides with your initials (legibly)
c.  Label initial slide in the group with the cassette color and page 
number (this slide is first in the rack and faces forward for the Stainer 
person to see)
d.  NOTE:  If the block must be re-soaked to complete the slide count, then 
isolate the block on the ice tray by putting it at the back of the tray facing 
backwards. This will indicate that some slides have been cut previously.
8)  Place the slides for HE in the HE staining rack
a.  Write the color 

RE: [Histonet] RE: back up cryostat

[Ingles Claire]

I think one of the most important things to have a back-up for is a processor.
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Manfre, Philip
Sent: Fri 9/21/2012 7:15 AM
To: barbara.cr...@lpnt.net; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: back up cryostat



If you use anything often enough, it is wise to have a back-up, if possible. 
Microtomes, and especially cryostats, tend to malfunction without warning and 
can leave you stranded. We have a back-up that isn't ours, but we have access 
to it.  That's another option.  Make friends with someone else who has one and 
strike an arrangement with them.

Philip Manfre, BA, HT(ASCP)
Associate Principal Scientist
Merck Research Laboratories
WP45-251
PO Box 4
West Point, PA 19486

215-652-9750
215-993-0383 (fax)
philip_man...@merck.com





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RE: [Histonet] RE: mouse testis in Bouins/Picric acid hazzards

[Ingles Claire]

Yes, but why take the chance. There are also other chemicals in the lab the 
picric acid can interact with to make it even more volitile than it was to 
begin with. Dynamite other explosives have the same problem. The older it gets 
the more degraded and unstable it becomes. One never knows if or when. I'd like 
to avoid traumatically amputating my arms if possible, thank you.
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Geoff
Sent: Mon 9/17/2012 9:26 AM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: mouse testis in Bouins/Picric acid hazzards



I am with Wayne on this one. While I have not tried to make it explode
it does seem to me that the dangers are hyped beyond reason.
Years ago an old bottle of picric acid would be discovered in a high
school chemistry lab. Horrors! Call the bomb squad! So it was taken out
to a large field, packed
with explosives and BOOM! Of course it exploded, it was surrounded with
explosives.

Geoff

On 9/14/2012 8:58 PM, E. Wayne Johnson wrote:
 What danger of Picric Acid are you concerned with?




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RE: [Histonet] Unsubscribe

[Ingles Claire]

I vote for Raspberry! 
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of nmhi...@comcast.net
Sent: Tue 7/24/2012 11:36 AM
To: Stacey Crenshaw
Cc: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Unsubscribe



OH NO!  They're BACK!  Saints preserve us!




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RE: [Histonet] (no subject)

[Ingles Claire]

I'd love to know too. We also use a xylene substitute.
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Brendal Finlay
Sent: Mon 7/23/2012 12:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)




Hello all!

We are budgeted to get an automated coverslipper for our department. 
When we talked about it with our service rep, he asked if we wanted a
glass or tape coverslipper.  I worked with a glass one many years ago
when they first came out and all I remember is breaking and sticking
coverslips.  I'm sure things have improved by now and was wondering
about the pros and cons of the glass versus tape coverslipper.  Which
do you prefer and why?


Thank you!
Brendal C. Finlay, HT (ASCP)
West Florida Medical Center Clinic
brendal.fin...@medicalcenterclinic.com


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RE: [Histonet] ER, Clone SP1

[Ingles Claire]

Don't give anyone any ideas!!
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Jonathan Cremer
Sent: Fri 7/13/2012 1:45 AM
To: Morken, Timothy; Kim Donadio; Patsy Ruegg
Cc: Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] ER, Clone SP1



That drives me up the wall as well. How can you possibly patent something that 
has been made by nature, and in theory is present in every single human being 
(I'm talking about any random gene here)? That would be like Newton patenting 
gravity and then going around and charging everyone a licensing fee for staying 
on earth instead of floating off in space, or claiming every apple that has 
fallen off a tree.
It's ridiculous!
---




 


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RE: [Histonet] Mohs

[Ingles Claire]

I would love to know this too. I am getting about $23/hr but I have pretty good 
benefits. I'm at UWisc Madison Hospital.



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Rebecca a. Johnson
Sent: Mon 7/2/2012 5:37 PM
To: histonet
Subject: [Histonet] Mohs 




Need to know what Mohs techs are getting paid. 


Thanks
Becky
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RE: [Histonet] Humidity Check

[Ingles Claire]

I think it is actually a requirement that temp and humidity are monitiored 
daily. 
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of 
lau...@blufrogpath.com
Sent: Mon 7/2/2012 3:19 PM
To: Histonet post
Subject: [Histonet] Humidity Check




   Do others check the humidity of their Histo lab on a daily basis?=

  




  


   Laurie Colbert
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RE: [Histonet] Frozen Sections Falling Off Slides

[Ingles Claire]

Just remember - No formalin, no need for retrieval. In my experience that is 
the hardest step on tissue sections. It also shortens the procedure a bunch 
too. 
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Emily Sours
Sent: Sun 6/17/2012 2:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Frozen Sections Falling Off Slides



Wow you can run frozen sections through those machines? That's awesome.  I
figured it wouldn't be gentle enough to process them.
I'm a fan of Fisher Scientific superfrost plus slides.  Fisher claims the
slides are electrostatically charged to keep the sections from falling
off.  Whether that's a bunch of hooha or it's actually true, our sections
don't fall off, even after in situs.  I believe most scientific suppliers
sell their own brand of superfrost plus slides if you don't want to buy
from Fisher.

Emily

You see a peanut, day's off to a good start; you witness some soil it's a
jamboree for Vince Noir.
--Howard Moon, in Charlie, The Mighty Boosh



On Sat, Jun 16, 2012 at 12:12 PM, Kim Donadio one_angel_sec...@yahoo.comwrote:

 Was your leica set that cold? Maybe you could try warming the slide for a
 couple seconds before putting in alcohol. As far a charged slides I am
 amazed with the slides Dako sells for their IHC.

 Sent from my iPhone

 On Jun 16, 2012, at 12:01 PM, Bernadette del Rosario badzros...@yahoo.com
 wrote:

  Hello histonetters!!!Im presently using the Tissue Tek Cryo3 Frozen
 section Machine...But i dont understand why i got problems sections falling
 off slides.I used different types of slides non adhesive,adhesive(poly l
 lysine,thermoshandon,starfrost sakura)..Regular procedure : Sections taken
 from 2-4 microns,transfered to 95% alcohol fixative, wolfsgang fixative
 etc..then after distilled water wash out...sections started to peel
 off...My machine setting- chamber is -30 C;cryochamber is -39 C..example of
 tissue- fibroma and salivary gland...
  I used Leica cryostat previously but i never got these problems...Even i
 used non adhesive but sections still on the slides..Can someone advised
  please.Maybe youre using the same tissue tek cryo 3...
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RE: [Histonet] Billing IHC on MOHS

[Ingles Claire]

Is this per Specimen or per surgical site. Mohs specimens are usually cut 
into smaller pieces and inked after the excision is removed. I would think this 
constitutes x# of blocks from the same specimen. Same as an excision that is 
breadloafed into separate sections. I don't think it matters who does the 
cutting, although when we send these to path for permenents they are logged in 
a separate specimens but only measured, never breadloafed.  Is it more 
dependent on how it is received in the lab even though the end result is still 
the same? 
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Weems, Joyce K.
Sent: Thu 6/14/2012 2:20 PM
To: 'Carol Torrence'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Billing IHC on MOHS



If each location is identified as a separate specimen, you can bill per 
specimen, is the way I understand it.


e.g.
Received separately - 88342 x 4

3:00 margin - A
6:00 margin - B
9:00 margin - C
12:00 margin - D

If one specimen is received and divided into separate cassettes  - 88342 x 1
A1 - 3:00 margin
A2 - 6:00 margin
A3 - 9:00 margin
A4 - 12:00 margin

Best, $1,783.00

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's 
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contain information that is privileged and confidential.  Any unauthorized 
review, use, disclosure, or distribution is prohibited. If you are not the 
intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Torrence
Sent: Thursday, June 14, 2012 2:37 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Billing IHC on MOHS

I have a question about billing IHC on MOHS.  When I go through the coding 
rules..I can defend it either way... I think. Ha!  If you are doing the same 
antibody on one site with 5 individual zones, taking 5 independently labeled
slides and each zone requires evaluation before continuing surgery.   Do you
charge 88342 times 5 or just once.  I understand it would be just once if this 
was a routine surgical specimen but this is a horse of a different color.



For example.  Even for frozen sections performed during surgery, additional 
margins can be charged as additional frozen sections.


Thanks in advance!



Carol M. Torrence, HT(ASCP)CM



ctorre...@kmcpa.com





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RE: [Histonet] ***News Flash*** OT

[Ingles Claire]

Not to TRY to offend anyone who actually has Leprosy, but Wierd Al Yankovic 
has a song titled Party at the Leper Colony. The puns are really groaners. 
Almost makes me wish I had the disease so my ears would fall off... :) I'll 
find my copy (yes I am a big Wierd Al fan) if anyone is actually interested 
in hearing it. 
He also has a song called I Love my Pancreas set to the beat of Beach Boys 
type music. There is another medical one but I can't recall it right now.
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Joe Nocito
Sent: Tue 6/12/2012 4:33 PM
To: 'Kim Donadio'; 'Jay Lundgren'; 'histonet'
Subject: RE: [Histonet] ***News Flash***



My question is are they being found with empty Lonestar beer cans. Inside
joke. Years ago in Texas, Lonestar Beer was running commercials with
armadillos running across the street carrying Lonestar Beer. See, down here
in Texas, armadillos are usually found as road kill. The commercials had
them belly up holding a Lonestar Beer bottle. I used to have a stuffed one
in my truck. Only in Texas!!!

JTT

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Donadio
Sent: Tuesday, June 12, 2012 4:24 PM
To: Jay Lundgren; histonet
Subject: Re: [Histonet] ***News Flash***

Epidemic update***
 
Seems the Critters have been tracked back to south florida. Witnesses have
described multiple critters on the sides of highways feet pointing up small
children assumed the critters had passed from a heart attack. No injuries
have been reported but one arrest was made. One man was caught surgically
removing the brain from one such critter. Homeland security has been
notified. Stay tuned for further details.



 From: Jay Lundgren jaylundg...@gmail.com
To: histonet histonet@lists.utsouthwestern.edu
Sent: Tuesday, June 12, 2012 2:14 PM
Subject: [Histonet] ***News Flash***
 
BREAKING NEWS
I. C. Critterz
The Associated Press

 Washington, D.C.-  Residents of northern D.C., along with Bethesda,
Silver Springs, and College Park are reporting a wave of armadillo
sightings.  The armadillos are allegedly covered in unusual skin lesions
and missing several toes.  Local zoologists are baffled, as armadillos are
native to the south-central and southeastern United States and are
not normally found in Maryland.  While no attacks have been reported,
authorities are urging residents not to approach any armadillos they might
encounter.
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RE: [Histonet] Can use some help with processing references

[Ingles Claire]

We have also removed the blocks and let them harden until we are ready to embed 
them, then put back in the hot well on the embedder to warm back up and go from 
there. As they are already processed and 
infiltrated, I don't think there would be a problem leaving them at room 
temperature for a few days.
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Ian R Bernard
Sent: Sun 5/13/2012 4:48 PM
To: Jackie O'Connor; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Can use some help with processing references



Rather than research literature (lit), may I refer you to the foremost 
authority on histotechnology procedures: Histotechonology- A Self Instructional 
Text, 3rd Edition by Frieda Carson and Christa Hladik, Chapter 2, page 38, 
paragraph 2: Tissue should remain in paraffin the shortest time necessary for 
good infiltration because exposure to prolonged heat causes shrinkage and 
hardening.  I consider book the foremost (bible) reference on histotechnology. 
 At least I hope so, since I'm studying for my HTL from this book. Note: There 
are lit at the end of this chapter that may go into more detail.

The paragraph goes on to state that:  ...melted paraffin should be kept 2 to 4 
degrees Celsius above the melting point because tissues exposed to overheated 
paraffin during infiltration will over harden.

Bottom-line, I would not leave tissues in paraffin over the weekend.  If you 
are using an automatic-closed system processor, your equipment should be able 
to work on a delayed status, where the tissues will sit in 10 Neutral Buffered 
Formalin under vacuum (better for the tissue), all weekend, and start 
processing schedule on Sunday to come off on Monday morning. At least ours does.

Hope this helps.

Ian R. Bernard
Ian R. Bernard, MSHA, HT (ASCP)
10th Medical Group- Anatomic Pathology Lab
USAF Academy, CO 80840



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jackie O'Connor
Sent: Sunday, May 13, 2012 2:42 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Can use some help with processing references



Hi Y'all - I'm kind of in a jam here - I'm looking for references on paraffin 
tissue processing - can someone point me towards any literature that says 
leaving tissues in molten paraffin over the weekend is a bad idea?  I need to 
prove a point, and need something to back me up.  I'm out of the US doing a 
consult, having trouble doing a lit search - I'm really not lazy. 
Thanks in advance.
Jackie O'
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RE: [Histonet] RE: Qualifications for grossing

[Ingles Claire]

You can reprocess, recut, and restain, but never re-gross. I for one also 
gross, but only skin. And yes, I DO know how to gross an alopecia specimen.
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Rene J Buesa
Sent: Wed 4/25/2012 11:55 AM
To: Joanne Clark; Davide Costanzo
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: Qualifications for grossing



For what little it may mean, I wholeheartly agree with Davice Costanzo e-mail. 
I completely agree with him.
In the same way the economic situation we are now was caused by greed, that is 
the motor guiding those who, to just save money, let a histotech (ologist) to 
do grossing.
Grossing,, especially large complex specimens, is the fundamental initial task 
in any complex diagnosis. The PA is the one who SELECTS what is going to be 
processed and used for diagnosis.
IF some part of the specimen is not submitted as the result of ignorance caused 
by poor training, the worst thing could happen, namely, a FALSE NEGATIVE

--- On Wed, 4/25/12, Davide Costanzo pathloc...@gmail.com wrote:



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[Histonet] While on the topic of harmful chemicals...

[Ingles Claire]

I have been having some health issues lately (understatement) Does anyone know 
the best formalin resistant disposable gloves? I gross (skin only) under a fume 
hood but the gloves still come in contact with formalin naturally. I am also 
(OK, mostly) interested in the health effects involving formalin, Propar, and 
the alcohols as they pertain to the liver. (No guys, I mean REAGENT alcohols, 
not the ingestable kind.) :)
Any direction I can be pointed in would be great. I am praying I won't have to 
find a creative way of practicing my beloved career because  these chemicals 
are causing or making worse my health problems.
:(
 
Claire

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[Histonet] (no subject)

[Ingles Claire]

Hey histo gurus:
Just a little twist for you. We are planning on doing GMS and PASD on frozen 
skin sections. Is there any change in staining protocol for paraffin vs frozen. 
We plan on fixing in ETOH. I have a co-worker saying that we don't need to use 
diastase on the PASD because there are already active enzymes in the tissue 
as it is fresh. He also said that we don't need to heat the silver for the GMS. 
Not sure why. These changes don't seem right to me. But I have been wrong 
before (Yes, I admit it). Thanks for all the wisdom out there floating in the 
electrons. We are staining for fungus BTW.
Claire

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RE: [Histonet] anti static spray?

[Ingles Claire]

 
Are you sure it isn't just your electric personality? :)
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Kim Donadio
Sent: Fri 2/10/2012 2:15 PM
To: Elizabeth Chlipala
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] anti static spray?



I have this problem a lot. I have static so bad literally blue sparks fly from 
my finger tips. I'm scared I'm going to blow up something one day and I walk 
around terrified to open doors. Anyway, in order to remedy my problem during 
sectioning I use a lot of hand lotion and get some bounce dryer sheets and run 
over my cloths and hair This usually works.
Kim D




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RE: [Histonet] Stain and component shelf life

[Ingles Claire]

Can you share this info with me too? We just got grief about this from our JC 
inspector about a month ago.
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Breeden, Sara
Sent: Tue 12/13/2011 8:04 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Stain and component shelf life



Can someone direct me to a reliable source for information on shelf life
of stains and components, specifically expiration date for unopened vs.
opened powdered/dry/granular stains?  I have heard unopened dry stains
w/shelf life of 10 years; opened dry stains w/shelf life of 5 years. Any
citation for that?



Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)



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RE: [Histonet] Toluidine blue

[Ingles Claire]

Anyone know Dutch?



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Bruijntjes, J.P. 
(Joost)
Sent: Fri 12/9/2011 4:47 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Toluidine blue



Henk

Al weer even geleden: weet je al iets over de toepassing van CD4 op 
varkensweefsel formaline gefixeerd en in paraffine ingebed weefsel?

Heb nog een vraag: zijn jullie in het bezit van een cryostaat waar je nog met 
ouderwetse vaste messen kan snijden? Ik ben op zoek naar de mogelijkheid om 
m.b.v. een D-mes bothoudend/verkalkte weefsels te snijden. We hebben hier een 
microm, maar die is zoals die hier nu staat alleen toepasbaar voor disposable 
mesjes. Een aanpassing om vaste messen te gaan gebruiken kost een ruime 1000 
euro en als het niet zou werken is dat zonde-geld.

Alvast bedankt
Joost

TNO.NLhttp://www.tno.nl/

Joost Bruijntjes

T +31 88 866 17 38
F +31 30 694 49 86
E joost.bruijnt...@tno.triskelion.nlmailto:joost.bruijnt...@tno.triskelion.nl

Disclaimerhttp://www.tno.nl/tno/email/


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RE: [Histonet] Fibulin 3

[Ingles Claire]

Does this antibody detect little lies?
 
Sorry, short week and am anticipating the turkey!
Claire
 
 

Subject: [Histonet] Fibulin 3

Does anyone have any experience with Fibulin 3 (EFEMP1) IHC in human tissues? 
Interested in opinions/observations on staining patterns  and sub-cellular 
localization
Thanks in advance and happy thanksgiving to all





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RE: [Histonet] RE: 15 years of Histonet

[Ingles Claire]

 
I didn't think space had a side anyway.?.  You're not an egomaniac, You are 
just really proud of the work you do!
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Joe Nocito
Sent: Mon 11/14/2011 4:36 PM
To: Morken, Timothy; Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: 15 years of Histonet



Can't agree more. I mean, where would Joe the Toe be without the Histonet?
By the way, just read the reviews from the lecture my buddy Hector and I
gave in Cinci this year. One of the critiques stated that Hector and I are
the biggest egomaniacs this side of space. Wow, I've been called a lot of
things in my life, but an egomaniac? Really? That hurt, right to the core.




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RE: [Histonet] Luciferase IHC

[Ingles Claire]

And it's not even Friday!
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Kim Donadio
Sent: Tue 11/1/2011 7:00 PM
To: Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Luciferase IHC



You have to soak it in holy water, then all H311 will break out
 
Sorry, I couldnt resist.
 
 





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RE: [Histonet] RE: Leica Bond verses the Ventana Ultra

[Ingles Claire]

James Bond? Sean Connery was always my favorite. :) Happy Friday! I'm off for a 
week and a half to the northwoods. Those fish better look out, and I don't mean 
zebrafish.
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Rathborne, Toni
Sent: Fri 9/16/2011 11:33 AM
To: 'Horn, Hazel V'; 'Pardue, Judith'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Leica Bond verses the Ventana Ultra



We love our Bond, but can not compare to the Ultra for long-term use. We demo'd 
both instruments, and chose the Bond.




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RE: [Histonet] Biocare decloaker

[Ingles Claire]

I swear I blow a gasket at least once a week!   Oh wait that's me, not the 
decloker. :)
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Weems, Joyce
Sent: Mon 7/25/2011 3:42 PM
To: William; Mike Pence
Cc: histonet@lists.utsouthwestern.edu; Diana McCaig
Subject: RE: [Histonet] Biocare decloaker



I wonder if a new gasket would help me.. I keep blowing mine! :)


Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of William
Sent: Monday, July 25, 2011 16:40
To: Mike Pence
Cc: histonet@lists.utsouthwestern.edu; Diana McCaig
Subject: Re: [Histonet] Biocare decloaker

As to the efficacy of retrieval, there is actually not that big of a 
difference. Biocare publishes that 90 degrees for 60 mins is roughly equivalent 
to 125 degrees for 30 seconds.

My guess, based on limited information in the original email is that Diana 
needs a new gasket. Those should be replaced every 6 months.

Will Chappell

Sent from my iPhone

On Jul 25, 2011, at 1:34 PM, Mike Pence mpe...@grhs.net wrote:

 There is a huge difference between 122 degrees for 30 seconds and 90
 degrees for 45 minutes. I would say you need to look at something here!

 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana
 McCaig
 Sent: Monday, July 25, 2011 2:45 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Biocare decloaker


 We are trying to validate a Biocare decloaker and have found when we
 use
 122 degrees for 30 seconds we get great signal, but distorted
 morphology.



 If we reduce to 90 degrees for  45 minutes, the signal is
 significantly decreased but the morphology is good.



 What protocols are you using?



 Diana

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It may contain information that is privileged and
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RE: [Histonet] How many tissues an histo tech is suppose to cut per

[Ingles Claire]

 
I frankly would like to be able to not worry so much about TAT so the larger 
tissues can fix better! 
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Grantham, Andrea L 
- (algranth)
Sent: Tue 6/28/2011 10:05 AM
To: undisclosed-recipients
Cc: HISTONET
Subject: Re: [Histonet] How many tissues an histo tech is suppose to cut per



I have to jump into this discussion if only to say that I am in total agreement 
with Susan and others regarding quality over speed.
Over the last few years I've had many students rotate through my lab - a 
research core facility -  and when I'm teaching them to cut the perfect section 
they tell me that in the clinical labs they don't have time for perfect. It is 
sad that we can't all strive to be the best that we can be especially when the 
outcome of what we do has a huge impact on a patient's treatment in many cases. 
When I was growing up in histology I had a pathologist who impressed on me 
the importance of good sections. He said the job of the pathologist is hard 
enough without trying to read out slides that are less than optimal and this is 
what you get when you rush through the sectioning.
Just try to cut one slide per minute and see what your pathologist has to say 
about the sections.
Andi








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[Histonet] CE certification

[Ingles Claire]

Question to all you ASCP rule gurus - I got my certification before the 
stipulation involving having so many CE's in order to get recertified every 3 
years. In order to be current with my certification, do I still need to get 
recertified every 3 years or am I grandfathered in to the old rules where I 
don't have to and my certificate is essentially good 'forever'? My boss is 
trying not to give me my certification bonus because he says I am not current 
on my certification. Not to be greedy, but $300 is $300.
Claire 
 

 

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RE: [Histonet] Retirement

[Ingles Claire]

But what about wildfires? :)  
Otherwise send me an application. I have been to New Mexico (mostly Abiquiu 
area) a few times and loved it. Not too sure about Alberquerque though. Too 
many episodes of Cops based there. Taos is nice enough. ;)
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Breeden, Sara
Sent: Fri 6/17/2011 1:08 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Retirement



It seems that my reference to RETIREMENT has gotten everyone thinking
about it.  Heh...heh..  It has been suggested that I  reconnoiter in
advance of Those of You Who Won't Be Retiring Before I Do (February 29,
2012, if the creek don't rise...).  I would be happy to perform that
hazardous duty but I need more of those $5.00 donations coming in for
whatever it was that I posted last week (I hope my gray cells will
rejuvenate when I retire).  I won't need the money for travel because I
think I'm right where I need to be.  Have any of you thought about New
Mexico???  Just within the past year, it has occurred to me many times
why this is such a good choice for retirement.  We do not have
hurricanes, we do not have tornados (okay, maybe rarely), we are not
prone to earthquakes, the weather is jolly darn good 90% of the time
(spring is out - way too windy) and we don't have more than a couple or
four inches of snow in the winter.  We don't start our furnace/heater
until November and it's only in use until maybe early April.  The air
conditioner was just put to use two weeks ago and we won't need it past
the first part of September.  Low cost of living, lots of homes
(reasonably priced - info upon request) for sale and the number of
things one can do in New Mexico are practically endless.  We have
everything but a beach (and if California keeps shaking, we might have
beachfront property - not that I'd wish that on California...).  We have
skiing, a big lake (fondly called Elephant Butt [Butte]) for water
activities, stream, river and lake fishing out the kazoo, mountains to
climb, white sands in which to wallow, beautiful sunsets and terrible
drivers.  Oops - that one slipped out!  The margarita (and Bud Light)
are the State Drinks (if one is so inclined) and this is the Land of
Manana (read it like Spanish).  Manana is way much better than I needed
that right this very minute and no excuses!  Shaded patios, cool
evening breezes and gorgeous cool summer mornings (at least until 7:00
a.m.).  Besides, I need a replacement beginning March 1, 2012.  Brand
new lab, tech-designed, bright and LEED, tons of space, a separate
storage room for blocks and slides AND a volatile storage room with two
acid cabinets and two xylene/alcohol cabinets and a salary (that's
another subject, I do work for a State...).  Can't have everything, but
this is darned close.



And I do not work for the Chamber of Commerce!



Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)



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RE: [Histonet] RE: Retirement

[Ingles Claire]

Try about 30 (years)!
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Blazek, Linda
Sent: Tue 6/14/2011 2:25 PM
To: 'Breeden, Sara'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Retirement



7 years compared to 8 months is gigantically significant.





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RE: [Histonet] IHC pos. neg. control question

[Ingles Claire]

 
And the block in question has already been proven positive using THAT procedure 
and antibody during validation.
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Thomas Jasper
Sent: Thu 5/19/2011 2:39 PM
To: pete.peder...@healthonecares.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos.  neg. control question



Pete,

When you run a positive control.  The tissue is already a known positive
(or it should be) for whichever antibody you are running regardless of
prior handling.  It would be impossible for this not to be so.  However,
with a negative, the concern is seeing how the patient tissue turns out
when subjected to all the same conditions, minus the antibody. 
tj

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
pete.peder...@healthonecares.com
Sent: Thursday, May 19, 2011 12:31 PM
To: gdaw...@dynacaremilwaukee.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos.  neg. control question

Glen,

If I am to understand you correctly you are saying control tissue is not
treated the same as patient tissue, therefore is useless as a negative
control correct? Then inversely doesn't that mean the same thing towards
the use of a positive control? How can you guarantee the positive
control tissue was treated the same as the positive stained patient
tissue? According to your logic it cannot. Therefore, without the use of
a negative control how can you say the staining seen in the positive
control is truly positive and not artifact? Best practice says use
positive and negative patient and control tissue. Please enlighten me if
you know anything to the contrary?

Pete Pedersen   B.S. HTL (ASCP)
Anatomic Pathology Supervisor

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dawson,
Glen
Sent: Thursday, May 19, 2011 12:32 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos.  neg. control question

IMHO: Running any piece of tissue as a control that does not belong to
the patient being tested makes zero sense.  Because it would not be from
the patient tissue being tested, how do you know if it was handled the
same as the patient tissue?  For example:

1) Were they processed the same way?
2) Did the patient tissue dry out in the OR before it was delievered?
3) Was the patient tissue ever irradiated?
4) Does the patient tissue contain any of a number of substances that
could cause non-specific staining.
5) Was the patient abducted by aliens?

My point is that running a piece of tissue as a negative control that is
not even from the patient being tested throws all of the conditions that
the patient tissue was exposed to prior to and during processing out the
window.  This makes NO sense.

Glen Dawson  BS, HT(ASCP)  QIHC
IHC Manager
Milwaukee, WI



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Curt
Tague
Sent: Thursday, May 19, 2011 11:04 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC pos.  neg. control question

I got this email from a pathologist today. we have always run a positive
with the patient tissue and a negative, the same patient tissue, and had
no
problems. Am I missing something. Is there any documented regulation
dictating what needs to be used for the controls. In some cases if we
get
one slide of patient tissue, then we will use the pos. and neg. cont.
from
the same block but typically it's the pt. tissue that is used for the
neg.
control. Thanks for your guidance.



Email:

I received slides on sentinel lymph node biopsies with a positive
control
on the same slide as the breast tissue, but the negative control was
just
the patient's lymph node and did not have the corresponding section used
for
the positive control.  The patient's own tissue cannot be used as a
negative
control.  The tissue that stained positively must serve as the negative
control without the antibody.  This is critical and you need to correct
that
immediately.





Curt



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RE: [Histonet] RE: OT: April Fool's Nonsense

[Ingles Claire]

I get a bit creative with my joke slides and actually use old tissue to cut out 
shapes . Process, embedd, etc. and put them on slides and give to the doc. in 
the middle of his other cases. I have a pumpkin face on one with the slide 
labeled O'Lantern, Jack etc. I have also been known to use those cute bits of 
different shaped confetti and taped them to a slide for the docs. 
(coverslipping doesn't work too well as they are so thick).
Claire

 

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RE: [Histonet] expired abs

[Ingles Claire]

I think my abs expired long ago!
Happy Friday
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Patsy Ruegg
Sent: Thu 3/10/2011 8:28 PM
To: 'ihcrg Group (E-mail)'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] expired abs



Just wanted to remind everyone if you are about to discard expired abs I
would be willing to take them and put them to good use. 



If you have expired abs to get rid of contact me and I will provide you a
fedex acct. # so you can send them to me rather than discard.  This effort
paid off recently because I sent a care package of expired abs and detection
reagents to a hospital tech in Ghana West Africa who is using them to test
patients there because they have training in ihc but do not have the abs or
detection reagents to do it.



Regards,

Patsy





Patsy Ruegg, HT(ASCP)QIHC

IHCtech

12635 Montview Blvd. Ste.215

Aurora, CO 80045

720-859-4060

fax 720-859-4110

www.ihctech.net

www.ihcrg.org



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RE: [Histonet] Re: saliva for glycogen hydrolysis

[Ingles Claire]

Wouldn't GUM be a more palatable option? Who knows where some of those rubber 
bands have been!
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Robert Richmond
Sent: Mon 2/14/2011 11:09 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: saliva for glycogen hydrolysis



Here's a truly hi-tech suggestion for doing PAS-diastase stains by the
spit method (which by the way is still how it's done by the small
pathology services I work on):

You can produce copious quantities of saliva by the simple expedient
of chewing on a rubber band for a couple of minutes. This used to be
the technique used to obtain saliva samples for determination of ABH
substance secretor status in the blood bank. (I belong to that elite
20% of donors who are non-secretors, and Lewis-a positive to prove
it.)

Bob Richmond
Samurai pathologist and occasional sialogogue
Knoxville TN

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RE: [Histonet] Wage question for Wisconsin

[Ingles Claire]

 
The answer to that question may have to wait a week or two. At least here at 
the UW. Our wonderful governor is trying to get rid of the state workers unions 
and collective bargaining. He is trying to push it through (it was just 
introduced this last Friday), and the vote is slated for later this week. The 
techs at the UW hospital have fortunately signed their contract that is good 
until the reopener in 1013 and we are not strictly state employees. However, 
the way things are looking, it is not out of the question that he may also try 
to void our contracts.  Our pay MAY go up, but we will no longer have much, if 
anything, for benefits.  Don't count on sending your kids to the UW colleges if 
this goes through. 
Sorry, I'm fighting mad right now. I'm not usually big on the unions, but this 
is HUGE for millions of Wisconsinites. GGR!
Claire

Were there any other Wisconsin techs at the rally today?  Even the Police and 
Firefighters unions were down there! 


From: histonet-boun...@lists.utsouthwestern.edu on behalf of Michael Hillmer
Sent: Tue 2/15/2011 5:02 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Wage question for Wisconsin



We are dermatology clinic in Northeast Wisconsin and we are trying to do
gather accurate wage data.  Can anybody offer wages for an HT, HtL and
lab assistants?  Any help would be greatly appreciated.


Thank you-



Michael Hillmer PHR

HR Coordinator

Dermatology Associates of Wisconsin

Phone: (920)683-5278




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RE: [Histonet] Help with OCT problem

[Ingles Claire]

Same with ETOH. We wipe our surfaces down with it to clean them so no cross 
contamination as we freeze our skin here on the metal side areas of our 
cryostats. If the alcohol hasn't been wiped dry or evaporated we have that 
problem too.
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Liz Chlipala
Sent: Thu 2/10/2011 1:49 PM
To: Reynolds,Donna M; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Help with OCT problem



Donna

I have seen soft or rather sticky OCT samples in the past.  If you
freeze in isopentane and don't let the isopentane evaporate off the
samples prior to wrapping the sample in foil, that excess isopentane
changes the OCT, it makes it sticky.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com





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RE: [Histonet] Seeking a Surgical Pathology Supervisor in Madison, WI

[Ingles Claire]

Sorry gang: the position in Madison wasn't the one I was thinking of. Disregard 
warning. It is the position for the surgical path manager at UW Hospital in 
Madison I was talking about.
Claire




 I think you are confusing this position with a different position in
 Madison. The supervisor in this position will oversee Histology
 including IHC and the gross room. There are no autopsies and no renal
 lab. The ideal candidate will have experience with LEAN or workflow
 redesign. I look forward to speaking with anyone who is qualified and
 would be happy to give you more details.

 Regards,

 Therese
 Slone Partners

 On Feb 8, 2011, at 6:35 PM, Ingles Claire wrote:

 Sorry Therese, but -
 Only consider this position if you like to be Really busy. As I
 respect all on this list. I will issue a warning that this position is
 much more involved than the 'ad' says. You would be overseeing the Histo
 lab, IHC lab, Renal biopsy lab, Grossing area, Dermatopathology lab, and
 have daily contact with the pathologists and many others. Contact me for
 details if you need to. Bob Merril (if anyone knows him) had this job,
 but he has retired.
 Claire
 UW Hospital and Clinics
 Madison WI

 

 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Therese
 Cook
 Sent: Mon 2/7/2011 9:21 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Seeking a Surgical Pathology Supervisor in
 Madison, WI




  Slone Partners=eeks a Surgical Pathology Supervisor for our hospital
  client, located=n Madison, Wisconsin.



 This  key  management position, will=versee the staff and the day
  to  day  operations of this busy department. =athology Assistants or
  HT and supervisory experience are required. A BS=s preferred.



 If  you  are  energetic,  have  great  communication=kills, and a
  positive attitude, this might be the position for you.



 Qualified  candidates  should send their resume to Therese=ook at
  [1]there...@slonepartners.com.



 If you do not meet these qualifications, but wish to be considered
 for  other roles in the laboratory diagnostic industry, please forward
  your=esume to Tara Kochis at [2]t...@slonepartners.com.



 All inquiries are kept confidential.

 References

  1.
 3Dmailto:there...@slonepartners.com?subject=New%20Candidate%20Request%2
 0%28Surgical%20Pathology%20Supervisor%20-%201455%29
  2.
 3Dmailto:t...@slonepartners.com?subject=New%20Candidate%20Request%20__
 _
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet




 SLONEPARTNERS
 THERESE COOK - EXECUTIVE RECRUITER

 Corporate Headquarters
 1521 Alton Road #638
 Miami Beach, Florida 33139

 TOLL FREE:877.419.1439
 DIRECT:   330.863.1054
 CELL: 330.323.4525
 FAX:  330.232.9333

 www.slonepartners.com




SLONEPARTNERS
THERESE COOK - EXECUTIVE RECRUITER

Corporate Headquarters
1521 Alton Road #638
Miami Beach, Florida 33139

TOLL FREE:  877.419.1439
DIRECT: 330.863.1054
CELL:   330.323.4525
FAX:330.232.9333

www.slonepartners.com




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RE: [Histonet] Seeking a Surgical Pathology Supervisor in Madison, WI

[Ingles Claire]

Sorry Therese, but - 
Only consider this position if you like to be Really busy. As I respect all on 
this list. I will issue a warning that this position is much more involved than 
the 'ad' says. You would be overseeing the Histo lab, IHC lab, Renal biopsy 
lab, Grossing area, Dermatopathology lab, and have daily contact with the 
pathologists and many others. Contact me for details if you need to. Bob Merril 
(if anyone knows him) had this job, but he has retired.
Claire 
UW Hospital and Clinics
Madison WI 



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Therese Cook
Sent: Mon 2/7/2011 9:21 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Seeking a Surgical Pathology Supervisor in Madison, WI




   Slone Partners=eeks a Surgical Pathology Supervisor for our hospital
   client, located=n Madison, Wisconsin.
  

  
This  key  management position, will=versee the staff and the day
   to  day  operations of this busy department. =athology Assistants or
   HT and supervisory experience are required. A BS=s preferred.
  

  
If  you  are  energetic,  have  great  communication=kills, and a
   positive attitude, this might be the position for you.
  

  
Qualified  candidates  should send their resume to Therese=ook at
   [1]there...@slonepartners.com.
  

  
If you do not meet these qualifications, but wish to be considered   for  other 
roles in the laboratory diagnostic industry, please forward
   your=esume to Tara Kochis at [2]t...@slonepartners.com.
  

  
All inquiries are kept confidential.

References

   1. 
3Dmailto:there...@slonepartners.com?subject=New%20Candidate%20Request%20%28Surgical%20Pathology%20Supervisor%20-%201455%29;
   2. 
3Dmailto:t...@slonepartners.com?subject=New%20Candidate%20Request%20___
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RE: [Histonet] Re: Storing acetic acid ***

[Ingles Claire]

 
GEE, and all us young, wet behind the ears techs thank goodness you are here to 
keep us on the straight and narrow.(OK, maybe not the second part...)  I learn 
so much from you guys.
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of John Kiernan
Sent: Tue 1/25/2011 2:04 PM
To: Robert Richmond
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Re: Storing acetic acid ***



 Schoolboys in the 1950s knew all about this and other interesting explosive 
chemical reactions, back in the days when elementary chemistry was more than 
just arithmetic. We had fun!




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RE: [Histonet] History of Histology

[Ingles Claire]

This book, and a couple of others by Mitchell, are availible for free if you 
have a Nook (Barnes  Noble e-reader). You won't believe all the older (circa 
late 1800's, early 1900's) histo  pathology books. Nothing by Brian 
Bracegirdle though. There are plenty of interesting reads if time permits. Most 
of them cheap or free.
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Victoria Baker
Sent: Tue 1/4/2011 9:34 AM
To: Geoff McAuliffe
Cc: histonet@lists.utsouthwestern.edu; Barone, Carol
Subject: Re: [Histonet] History of Histology



Thophil Mitchell Prudden A Manuel of Normal Histology  published in 1881.

Vikki




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RE: [Histonet] High temp/paraffin oven

[Ingles Claire]

 
Not as much room/air flow for vents to dissipate extra heat?
Claire

 


From: histonet-boun...@lists.utsouthwestern.edu on behalf of 
histopa...@hotmail.com
Sent: Wed 12/1/2010 4:19 PM
To: Histonet
Subject: [Histonet] High temp/paraffin oven



Hello to all,

Any idea why my middle paraffin oven would be out of range, but the othet two 
on opposite sides are not?

Histopathy
Sent on the Sprint® Now Network from my BlackBerry® 


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RE: [Histonet] Hellerstrom Hellman anyone?

[Ingles Claire]

Did you acid clean your glassware before hand?
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Amos Brooks
Sent: Wed 12/1/2010 12:07 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Hellerstrom  Hellman anyone?



Hi,
I just did a Hellerstrom  Hellman alcoholic silver stain for 
pancreatic D-
cells. I followed the method described by Bancroft  Stevens. When the slides
were placed into the reducing solution they instantly precipitated into a
toasty mess, the solution turned milky brown and the slides (even around the
sections) picked up a lot of silver precipitate. If anyone has done this of
could offer suggestions about it, I'd really like to trouble shoot the stain a
bit.
A bit of background about the stain that I thought I should add. 
According to
the published method I used a 10% silver solution in ETOH with 100ul of 1M
nitric acid. The pH of the solution was 5.0. The reducing solution was
alcoholic formalin with 5% pyrogallic acid.
I thought the silver solution seemed really concentrated (It hardly even
disolved). Incubating such a solution overnight at 37 deg C (as indicated in
the method) seemed a bit much. Has anyone heard of modifications of this
stain? There is no sense reinventing the wheel, but I certainly don't want to
repeat it knowing that it will innevitably do the same thing again unless
there is something glaringly obvious that I did wrong.

Thanks,
Amos

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RE: [Histonet] Un related to actual histology

[Ingles Claire]

Ah---A woman after my own heart. I always thought of going as Bunson Honeydew 
or (especially) Beaker from the muppet show.  I work in a clinic setting and 
have always thought of coming in as the nurse/ Daryl Hannah character from Kill 
Bill would be interesting, but I don't want to give the patients heart attacks 
or have all the nurses get offended and get me fired. I would also of course 
bring a tape recorder to play her theme as well as wearing the obligatory 
katana. 

Or even, FrankenSTEEN.  Stab a scapel in your thigh for full effect.  Also
bring a recording of horses neighing so you can say Frau Bleuker!

Emily
--


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RE: [Histonet] RE: Cutting, Processing, etc

[Ingles Claire]

DON'T get me started... And I have only been practicing 9 years!
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Nails, Felton
Sent: Thu 9/16/2010 11:06 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Cutting, Processing, etc




 what is happening to our field??

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R
Sent: Thursday, September 16, 2010 10:38 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cutting, Processing, etc

Hello Histoland!






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RE: [Histonet] Will a Thermo Scientific Sales Representative pleasecontact me

[Ingles Claire]

Maybe they're all pestering me. :) I believe Leica has Thermo now. I know they 
have closed at least one factory in my region, maybe more.
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Blazek, Linda
Sent: Wed 9/8/2010 3:40 PM
To: 'Horn, Hazel V'; 'gayle callis'; 'Histonet'
Subject: RE: [Histonet] Will a Thermo Scientific Sales Representative 
pleasecontact me



You mean there are ones?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Wednesday, September 08, 2010 4:20 PM
To: 'gayle callis'; 'Histonet'
Subject: RE: [Histonet] Will a Thermo Scientific Sales Representative please 
contact me

Ditto...I need the same help

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of gayle callis
Sent: Wednesday, September 08, 2010 3:14 PM
To: 'Histonet'
Subject: [Histonet] Will a Thermo Scientific Sales Representative please 
contact me

I need to have a Thermo Scientific sales rep contact me to clarify ordering
Richard Allan staining reagents. 



Thank you



Gayle M. Callis

HTL/HT/MT(ASCP)

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RE: [Histonet] caris and genoptix experience

[Ingles Claire]

Gee... It's not like WE have work to do or anything. Thanks for the heads up 
about these companies. 
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of 
kim.dona...@bhcpns.org
Sent: Sat 9/4/2010 10:55 AM
To: histot...@imagesbyhopper.com
Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] caris and genoptix experience



I had exactly the same experience with the same company.

It's a bad mark right off the bat with me if vendors just walk in the door
and expect me to drop what I am doing.






Kim Donadio
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996



histot...@imagesbyhopper.com
Sent by: histonet-boun...@lists.utsouthwestern.edu
09/03/2010 09:51 PM

To
'Tench, Bill' bill.te...@pph.org, histonet@lists.utsouthwestern.edu
cc

Subject
RE: [Histonet] caris and genoptix experience






Very interesting.  I recently had a very pushy Genoptix rep at my
facility.
Wanted to take a LOT of my time, but as I was walking out the door (the
rep
came unannounced), they only got about 10 minutes.  They were quick to
point
out that wasn't enough time ... well, um ...  make an appointment!!




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tench,
Bill
Sent: Friday, September 03, 2010 4:13 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] caris and genoptix experience


We have had almost identical experiences with both organizations. Caris
has
been informed that it is not welcome; genoptix is on the same list Bill
Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar
Medical Center 555 E. Valley Parkway Escondido, California  92025
bill.te...@pph.org
Voice: 760- 739-3037
Fax: 760-739-2604


[None] made the following annotations
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RE: [Histonet] Silver

[Ingles Claire]

I thought Histotechs were supposed to have purple thumbs. :) 
(You know, gardeners have green thumbs...)
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of O'Donnell, Bill
Sent: Tue 9/7/2010 4:15 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Silver




I know... I should wear gloves when doing a GMS.I... know... that.
(sorry, I thought this was Facebook for a second) Have a great week!

- Sir Bill of the Blackened Thumb


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RE: [Histonet] I Have been scammed

[Ingles Claire]

Glad you're not stuck, Jerry. This is why I act like an old codger and refuse 
to get a facebook,other social networking sites, etc. account. What's the deal 
with farmville anyway? :)
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Jerry Santiago
Sent: Tue 8/17/2010 10:52 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] I Have been scammed



Histonetters,

Thanks for all of your concerns and calls. I was made aware of the Europe scam
and have contacted the authorities and they are doing what they suppose to do.

I am still in Florida and have not left the country for any reason. My e-mail
company states that this was stolen from facebook and gained access to my email
account cleaning out all of my contacts and my access to my e-mail account as
well as facebook.

It will be fine and I apologize for anyone that got caught in this scam.

Jerry Santiago
Shands Jacksonville
Jacksonville, FL
904-244-6149
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RE: [Histonet] Retaining empty jars

[Ingles Claire]

Ditto.
Claire


re:

CAP retention guidelines say that wet tissues are to be retained for 2 weeks 
after final signout.  We keep all containers, empty or not, for the 2 weeks 
then strain and discard them in biohazard trash.




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RE: [Histonet] Antibody Validation

[Ingles Claire]

Funny, I'd think the vendors would want you to do tons of your own validation, 
as it uses up antibody faster, ergo they get to sell you more antibody! :) In a 
feisty mood today.
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Rene J Buesa
Sent: Tue 6/15/2010 9:49 AM
To: histo...@pathology.swmed.edu; teri.hall...@midmichigan.org
Subject: Re: [Histonet] Antibody Validation



Teri:
You are right about the validations you propose although I am not surprised 
that your vendor does not think it is necessary. They are in the business of 
selling and you are in the business of assuring the high quality of your work 
to obtaining the most accurate work for patients' sake.
There is where the difference resides. Ignore your vendor and keep validating 
your protocols.
René J.

--- On Tue, 6/15/10, teri.hall...@midmichigan.org 
teri.hall...@midmichigan.org wrote:


From: teri.hall...@midmichigan.org teri.hall...@midmichigan.org
Subject: [Histonet] Antibody Validation
To: histo...@pathology.swmed.edu
Date: Tuesday, June 15, 2010, 7:55 AM


I am being questioned by our vendor as to why we need to validate our
automated immunostainer and image analysis instrument. They would like
documentation pertaining to the requirement of validation and the number
of specimens utilized for validation.  I am requesting that each
antibody be validated on the instrument against a previously validated
instrument. Additionally, I am requesting that each new lot of antibody
be validated upon receipt against previously ran specimens. This would
also apply to the image analysis antibodies. (Her2 has been validated by
FISH.) The vendor has apparently polled users in the area and this is
not a standard protocol, therefore the request for documentation.

I think it is pretty clearly stated by CAP in the Quality Management In
Anatomic Pathology. Any other suggestions?

Teresa Hallada BS, MT/CT (ASCP)
Pathology Lead
MidMichigan Health - Gratiot
teri.hall...@midmichigan.org
989.463.1101 ext 3423

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[Histonet] Sodium borate?

[Ingles Claire]

Help!
Does anyone know a substitute for sodium borate in the GMS stain? Ours got 
unknowingly thrown out by our out-date police and he didn't tell anyone, and 
now we need it ASAP. And we don't have half of the stuff for the Warthin-Starry 
either. (Derm lab. We do this stuff oh so often, but now of course it is a stat 
case.) Or if anyone knows a halfway easy stain for, I'm assuming, spirochetes.
Claire
 

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[Histonet] Paraffin

[Ingles Claire]

A question has come up recently about whether or not to completely turn off our 
embedding center every night or not. I was under the understanding that the 
constant solidifying and re-melting in the paraffin pot damages the structure 
of the paraffin and makes it more brittle, etc. Is my memory correct, or do I 
need to update my hard drive?
Claire

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[Histonet] CD 133

[Ingles Claire]

Thought I'd try again...
Anyone know where I can send some slides for a CD133? 
Claire

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[Histonet] CD133

[Ingles Claire]

Happy Monday! (grumble grumble)
I need to know if there are any reference labs out there that do CD133 for stem 
cells. Our doc wants this done, but we can't find anyone who does it.
Thanks
Claire

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RE: [Histonet] Toluidine blue

[Ingles Claire]

Newcomer
 
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of 
zodia...@comcast.net
Sent: Wed 4/21/2010 5:35 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Toluidine blue



Hello to all,


I was wondering if anyone knows where to purchase toluidine blue (for mast 
cells) other than polyscientific.


Thank you in advance for your replies,


Jenny
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RE: [Histonet] ascp exam

[Ingles Claire]

Are you kidding!?! I have an AA in Histotechnology and still didn't pass my 
(HTL) written on the first round. I did pass my slide review though, thank you 
very much. :)  I passed on the second try, which included 3 years of OJT and 
tons of studying after graduating school. (BTW, I also have a BS in Biology 
with a major in English) I think they have just made the test harder because 
they have gotten rid of the slide part of the test. And it sounds like the 
micrographs still suck. :p
 
Claire
 
Disclaimer:  Chill out people. I'm not crabby, I'm just drawn that way.  



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Brandi Higgins
Sent: Wed 4/21/2010 6:18 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] ascp exam



Hello,

For those of you who are ASCP certified, either HT or HTL, did many of you
have to take the exam more than once?  I have not yet sat for the exam, but
I see the pass rate is low, and average score is usually just slightly above
the pass line.  I have read a lot of comments on some message boards saying
people don't even recognize the material in a lot of the questions, and how
the images of stains are of very poor quality.  I am just wondering if most
of the people who don't pass are people who think because they work in a lab
they will be able to pass the exam, and as a result they don't study for it
the first time.  Or, is it really that difficult and many people study hard
but still cannot pass it.  Any comments welcome!  Thanks!

Brandi Higgins
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RE: [Histonet] PA baby sitter

[Ingles Claire]

That's OK, our poor PA has to babysit the residents, so she needs all the help 
she can get!!
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Rene J Buesa
Sent: Tue 3/23/2010 5:24 PM
To: rick.garnh...@memorialhealthsystem.com; Jeffrey Silverman
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] PA baby sitter



Tell be about it again when you get to gross 38,000 cases/year.
René J,

--- On Tue, 3/23/10, Jeffrey Silverman pathmas...@yahoo.com wrote:


From: Jeffrey Silverman pathmas...@yahoo.com
Subject: [Histonet] PA baby sitter
To: rick.garnh...@memorialhealthsystem.com
Cc: histonet@lists.utsouthwestern.edu
Date: Tuesday, March 23, 2010, 5:18 PM


I had to laugh when I read this one. I'm a PA grossing 8500 surgicals per year 
in a busy general hospital so it's not just biopsies but lots of major organ 
resections with tons of orthopedics. I work with two histotechnologists. Not 
only do I close my own cassettes, I accession most specimens, make my own 
cassettes,  save and dispose of the surgical leftover tissues, serve as 
histology supervisor and laboratory safety officer for all sections attending 
all the associated meetings that those two entail, often embed at least half of 
the tissues and pitch in whenever I'm needed in histo- cutting and running 
automated specials.

Having said that, if there's anyone in Europe looking for such a person and can 
pay 60K euro per annum , I'd love to meet you.  I'm in the opposite boat of 
Malika. Hey, wanna trade jobs?

The techs are at my beck and call to bring me things I need,  like more acetone 
or formalin and/or  to attend to whatever other help I need, but no one ever 
sits with me to close the cassettes and feed me specimens.  I agree with the 
accountability issues involved in lost or mishandled tissue. What happens when 
the aide is off, do they expect  a histotech to come in to close the cassettes 
for the PA.
And the productivity increase for such assistance can't be more than 5% IMHO 
based on my experience. 

Jeff Silverman

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RE: [Histonet] Alcohol Source?

[Ingles Claire]

I vote for the scotch!
Claire




depends. A fine Scotch whiskey may be aged in old oak casks, while a
delicate Tequila may not be



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RE: [Histonet] Re: bone marrow fixative

[Ingles Claire]

Ah, in a perfect world! (which would be down right boring, thanks)
Claire


Neutral buffered formalin requires time for fixation - clots need to
be cut up as soon as possible after they're received, and biopsy
specimens really ought to fix overnight before decalcification and
processing. Communication with oncologists is essential (and rarely
achievable).

Bob Richmond
Samurai Pathologist
Knoxville TN

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[Histonet] Mohs FYI

[Ingles Claire]

Hey gang:
Here with some good news for once. I found out today that we WON our appeal to 
JCAHO over our tissue retaining time. They are giving Mohs labs an OK not to 
have to retain our tissue!! I guess it is 'processed' tissue instead of 'gross' 
tissue. Retaining it doesn't does not help patient care as it is not fixed and 
deteriorates (and there are already slides of it anyway.) 
So all you fellow Mohs techs can start breathing (and getting rid of your 
tissue) again.
Claire

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RE: [Histonet] cleaning cryostat

[Ingles Claire]

I work in a Mohs Lab. (high output frozen skin) We wipe out the cryostat with 
95% ETOH and clear out the shavings every day we use our machines. We normally 
tear down and completely defrost our cryostats every other month or so, 
depending on humidity and frost build up. (and available time to have a 
cryostat down.) We are not inspected by CAP, but JCAHO and CLIA who are at 
least as stringent. 
Claire




I would like this information also. This is the first year we are CAP so
any help I can get would be appreciated.  Thanks so much have a great
Friday and Week end. 
Kathy Gorham H.T.





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RE: [Histonet] Research Associate job opportunity (Danvers, MA)

[Ingles Claire]

Ian:
Love doesn't pay the bills these days. ;)
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Ian Montgomery
Sent: Tue 2/2/2010 8:46 AM
To: histonet@lists.utsouthwestern.edu
Subject: FW: [Histonet] Research Associate job opportunity (Danvers, MA)



John,
Its science, we do it for love, money is not an issue.
Ian.

Dr. Ian Montgomery,
Histotechnology,
I.B.L.S. Support Unit,
Thomson Building,
University of Glasgow,
Glasgow,
G12 8QQ.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of John Kiernan
Sent: 02 February 2010 06:21
To: Stephen Repucci
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Research Associate job opportunity (Danvers, MA)

Why doesn't your job ad say what the salary is?

- Original Message -
From: Stephen Repucci srepu...@cellsignal.com
Date: Monday, February 1, 2010 16:37
Subject: [Histonet] Research Associate job opportunity (Danvers, MA)
To: histonet@lists.utsouthwestern.edu

 Cell Signaling Technology, Inc. (CST) is a worldwide leader in
 the 
 development and commercialization of antibodies and assays for
 pathway 
 analysis, as well as novel discovery technologies such as 
 PhosphoScanR. We are committed to developing innovative new
 research 
 tools to help define the mechanisms underlying cell function
 and 
 disease, thereby broadly accelerating progress in biomedical
 research 
 and medicine.

 We are seeking a Research Associate in the Immunohistochemistry
 Group 
 to assist with the validation of antibodies for use in 
 Immunohistochemistry. Responsibilities will include 
 immunohistochemical analysis on paraffin-embedded tissues and
 cells, 
 but may evolve as the company continues to grow.
 Additional 
 responsibilities include protocol optimization/assay
 development, 
 discovery research, specialized staining projects, and may
 also 
 include product development. The candidate should be very
 well 
 organized with exquisite attention to detail and capable of
 multi-
 tasking and working independently in a fast paced, dynamic and
 high-
 volume testing environment. The ideal candidate will have an MS
 or BS 
 in a life science with at least two years of general
 laboratory 
 experience. IHC experience is preferred.

 For more information please contact me or apply on-line at:
 https://home.eease.com/recruit/?id=484960

 Stephen Repucci
 srepu...@cellsignal.com
 978-867-2318



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[Histonet] tissue retention...again

[Ingles Claire]

Hey there:
I would like to take a quick poll of all techs doing Mohs sections out there. I 
would like to know what you do with your tissue after being frozen sectioned, 
how long you keep it, how you store it, etc.
Thanks
Claire

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[Histonet] tissue retention times

[Ingles Claire]

OK, everybody crack open your JCAHO requirement manuals...
We are a MOHS lab and were surveyed by Joint Commission about 2 months ago. 
During the survey, we were cited because we only retain our physical tissue 
specimens for 24 hours, as they remain fresh and are not fixed. We throw the 
tissue the following afternoon vs. retaining for the 1 week after microscopic 
sections are examined and reports are reviewed and signed. (Standard 
#QC.2.120) 
Has anyone else (specifically a Mohs lab) been cited for this? We are currently 
trying to appeal, but have been denied so far. I am having a teleconference 
with the JCAHO powers that be to try and get the appeal going again, but I'm 
not the lawyer type.
The letter states that their interpretation is that even if the tissue is not 
regarded as 'gross' tissue, any 'useable' tissue must be retained for a minimum 
of a week after the case is signed off on.I am thinking on focusing on the 
'useable' bit as related to diagnostic value of delayed retained fresh tissue 
processing i.e. freeze artifact, tissue degredation, inability to perform 
ancillary testing, etc. Also, what is the definition of 'reviewed and signed 
reports'? Does this mean when the case is finalized, or just the tissue 
sections in question? We sometimes have patients that go a month + between Mohs 
procedures on the same positive lesion.
We will probably have to get a -80 freezer for this if the appeal doesn't go 
through. Not to mention the fact that some of our cases are not resolved for 
months. We would have constantly search when cases are finalized to know when 
we can dispose of the tissue. FYI, the letter also states that the JCAHO 
standard is more stringent than the CLIA standard, so I'm not even really going 
to use it in arguments. 
 
HELP!
Claire Ingles
UW Hospital  Clinics
Madison WI
 

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[Histonet] FW: FW: Follow Up: Joint Commission Lab Survey CorrectiveAction Plan West MOHS

[Ingles Claire]

OK Guys, here's another JCAHO/CLIA controversy. Has anyone (Mostly MOHS labs) 
been sited for this? Currently we only save tissue specimens 24 hours in case 
the Dr. decides to send some to path after the fact. (don't get me going on 
morphology quality). Does anyone have a clear definition of gross tissue 
specimens It's not like we measure this stuff before freezing.
Thanks for your well educated guesses
Claire


 

West MOHS Clinic was cited for a deficiency regarding maintenance and storage 
of tissue during the Joint Commission Lab Survey. 

According to standard QC.2.120, EP 2:  Microscopic slides, paraffin blocks, 
bone marrow aspirates, needle biopsy specimens, and gross tissue specimens are 
stored for required times as defined by organization policy, law, and 
regulation.  Microscopic slides, including stained slides, are retained for at 
least 10 years.  Paraffin blocks are stored for a minimum of two years from the 
date of examination.  Gross tissue specimens are retained for at least seven 
days after required microscopic sections are examined and reports reviewed and 
signed.  State law and regulation requirements are for longer times (See 
Appendix XX for additional specific retention times.)

The surveyor observed in his report that biopsy tissue was frozen for section, 
slides prepared and then the tissue was discarded.  Gross tissue specimens must 
be retained for seven days after the microscopic sections are examined and 
reports reviewed and signed.


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RE: [Histonet] Laboratory Licensing

[Ingles Claire]

Haven't heard of that one yet. Just so the Dr. is licensed to read the slides. 
Wouldn't it essentially (geographically) be the same as a Dr. reading consult 
slides from another hospital?
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Pratt, Caroline
Sent: Wed 11/4/2009 9:46 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Laboratory Licensing



We were recently surveyed by the Joint Commission.  We have our
laboratory at one physical address and our Dermatopathologists at
another physical address (the hospital).  The surveyor indicated that we
need a separate CLIA and state license for the hospital location where
the slides are being read even though no tests are performed there.
These are high complexity Tissue Pathology.  Has anyone heard of this
before or have a similar set up and how are you licensed.  It doesn't
sound accurate to me.  My understanding was that any test being
performed required licensure, I have never heard of laboratory licensure
being required for the reporting of results.  Please provide any
assistance or experience you can!!! Thanks!



Caroline M. Pratt, MBA




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[Histonet] storage temps

[Ingles Claire]

Hey gang:
Just a simple quiz. What are the temp. ranges for long term slide and (more 
importantly) paraffin block storage?
Claire

 

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RE: [Histonet] Plants windows in lab

[Ingles Claire]

I didn't think they had sun in Seattle. :)
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Blazek, Linda
Sent: Fri 10/23/2009 10:39 AM
To: histonet
Subject: RE: [Histonet] Plants  windows in lab




Don't go to the web site.  It has a picture of the water and boats!  It will 
just drive you batty!

-Original Message-



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RE: [Histonet] Inspection question/OT

[Ingles Claire]

And don't forget a colony of cochineal beetles...
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Edwards, R.E.
Sent: Thu 10/22/2009 9:14 AM
To: 'Thomas Jasper'; Weems, Joyce
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Inspection question/OT



Plants grown  in the lab might include, blue lungwort, dead man's fingers, 
mistletoe, cancer weed, devil's gut, bonewort, kidney vetch, liverwort, Hawaii 
birdnest spleenwort and if you  have  the space how  about a Haematoxylum 
campechianum?.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper
Sent: 21 October 2009 23:29
To: Weems, Joyce
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Inspection question

Furthermore, most any plant (unless I'm missing something here) is
beneficial for air quality...plants want CO2 and we appreciate their
oxygen.  I sure hope some inspector somewhere hasn't taken issue with
plants your in the lab.

Tom J.

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, Oregon 97701 
541/693-2677   
tjas...@copc.net

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems,
Joyce
Sent: Wednesday, October 21, 2009 3:17 PM
To: Patti Loykasek; histonet
Subject: RE: [Histonet] Inspection question

No...
And spider plants and some others help remove formalin fumes. That was
published somewhere. j


Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patti
Loykasek
Sent: Wednesday, October 21, 2009 18:15
To: histonet
Subject: [Histonet] Inspection question

Hi All. Happy Wednesday. Has anyone everyone had an auditor/inspector
note that plants in the histology laboratory are a possible
contamination hazard  must be removed? Just wondering.


Patti Loykasek BS, HTL, QIHC
Clinical Lab Supervisor
PhenoPath Laboratories
Seattle, WA




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you are not the intended recipient, please contact the sender by e-mail
and destroy all copies of the original message, or you may call
PhenoPath Laboratories, Seattle, WA U.S.A.
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RE: [Histonet] RE:Missing the point of Plants-in-the-lab OT

[Ingles Claire]

Sadly, our lab janitor could probably qualify as the same thing... And he's 
supposed to CLEAN the lab! Psoriasis skin flakes in a derm lab. Ewww.   Sigh :(
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Haynes, MaryAnne
Sent: Thu 10/22/2009 2:35 PM
To: Akemi Allison-Tacha; Sara''Breeden; histonet@lists.utsouthwestern.edu; 
LindaBlazek; Patti Loykasek
Subject: RE: [Histonet] RE:Missing the point of Plants-in-the-lab OT



Some Health departments state that plants and their potting soil can be a 
potential microbial and fungi contaminate in the lab.
Mary Anne Haynes

Mary Anne D. Haynes, MBHA,  DLM, SLS(ASCP)
Pathology Manager
Children's National Medical Center
Department of Anatomic Pathology
111 Michigan Ave NW
Washington, DC 20010
202-476-4311 (office)
202 476-4030 (fax)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi 
Allison-Tacha
Sent: Thursday, October 22, 2009 15:26
To: Sara''Breeden; histonet@lists.utsouthwestern.edu; LindaBlazek; Patti 
Loykasek
Subject: Re: [Histonet] RE:Missing the point of Plants-in-the-lab OT

Hi All,
I think all of you are missing the point of Patti's question.  She stated that 
her lab was dinged for having plants in the lab by a CAP inspector.  
I had the same thing happen to me years ago.  The inspector stated that plants 
attract insects that can contaminate a supposedly clean environment.  
Patti has an extremely well organized lab that only had a small phase (1) 
deficiency last year.  I think the inspector couldn't find anything, so they 
had to come up with this ridiculous infraction.

Akemi Allison-Tacha BS, HT(ASCP)HTL
PresidentPhoenix Lab ConsultingTele: 408.402.5257
Cell: 408.335.9994
E-Mail: akemiat3...@yahoo.com



--- On Thu, 10/22/09, Blazek, Linda lbla...@digestivespecialists.com wrote:

From: Blazek, Linda lbla...@digestivespecialists.com
Subject: [Histonet] RE: Plants-in-the-lab OT
To: 'Breeden, Sara' sbree...@nmda.nmsu.edu, 
histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Date: Thursday, October 22, 2009, 12:15 PM

I don't know Sally, but where I worked many moons ago I had a spider plant that 
was extremely prolific.  The powers that be made me remove it from the lab for 
an inspection.  It went to live in one of the administrator's office for 
several months.  And died!  I think it needed the fumes!  Or it missed me.

Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Phone: (937) 293-4424 ext 7118
Email: lbla...@digestivespecialists.com




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara
Sent: Thursday, October 22, 2009 3:02 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Plants-in-the-lab OT

I think it's the fluorescent lights that makes them thrive.  The
absorption of Fume Matter is a secondary, but beneficial, effect.  You
go, chlorophyll!



Sally Breeden, HT(ASCP)

NM Dept. of Agriculture

Veterinary Diagnostic Services

PO Box 4700

Albuquerque, NM  87106

505-841-2576



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RE: [Histonet] Inspection question

[Ingles Claire]

Not to mention a little organic bit of the outside world when your stuck inside 
a white-washed cube all day...
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Thomas Jasper
Sent: Wed 10/21/2009 5:29 PM
To: Weems, Joyce
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Inspection question



Furthermore, most any plant (unless I'm missing something here) is
beneficial for air quality...plants want CO2 and we appreciate their
oxygen.  I sure hope some inspector somewhere hasn't taken issue with
plants your in the lab.

Tom J.

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, Oregon 97701 
541/693-2677   
tjas...@copc.net

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems,
Joyce
Sent: Wednesday, October 21, 2009 3:17 PM
To: Patti Loykasek; histonet
Subject: RE: [Histonet] Inspection question

No...
And spider plants and some others help remove formalin fumes. That was
published somewhere. j


Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patti
Loykasek
Sent: Wednesday, October 21, 2009 18:15
To: histonet
Subject: [Histonet] Inspection question

Hi All. Happy Wednesday. Has anyone everyone had an auditor/inspector
note that plants in the histology laboratory are a possible
contamination hazard  must be removed? Just wondering.


Patti Loykasek BS, HTL, QIHC
Clinical Lab Supervisor
PhenoPath Laboratories
Seattle, WA




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RE: [Histonet] Iodine

[Ingles Claire]

Walgreens?



From: histonet-boun...@lists.utsouthwestern.edu on behalf of kristen arvidson
Sent: Tue 9/29/2009 4:01 PM
To: histonet
Subject: [Histonet] Iodine



I recently found out I can no longer get iodine from my usual vendor.  We use 
it for verhoff stain (we do our stains by hand).  Any suggestions??


 
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RE: [Histonet] Slide Brite xylene substitute

[Ingles Claire]

I inherited a couple gallons of slide brite. We have been using it for a month 
or two and is very inferior to other clearants we have tried. I just got sick 
of the drawbacks and got rid of it. It always seemed to leave water droplets on 
our slides and takes alot longer to clear. We changed back to Propar from 
Anatech. We have never had problems with it. We still use Permount on our 
slides but Anatech also has their Refrax mountant that is formulated to work 
better with Permount.  
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of jennifer cresor 
mike hough
Sent: Tue 8/11/2009 9:22 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Slide Brite xylene substitute



Hello all,

Does anyone out there have experiences with Slide Brite xylene substitute? Did 
you have any problems? Lately, I have been finding what appears to be water on 
my slides. Under the microscope it looks like pink gobules on the slide and the 
tissue. The last 2 days I changed out all of my stains and reagents to see if 
that would cure the problem and it did not. It is very random, not on all of 
the slides. But, when these gobules are on the slide it changes the stain 
dramatically to a dark blue appearing hematoxylin. Any thoughts? I am 
considering whether to change to a different xylene substitute. Any 
recommendations. Thank you for your response.

Jennifer
Temecula, California
jenc...@ca.rr.com
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