Re: [Histonet] reference lab in So. CAL

[Jennifer MacDonald via Histonet]

We were able to find a local lab that experience with the specimens in 
question.  Thank you to all that responded with offers of help.

Thank you, 
Jennifer MacDonald 
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Re: [Histonet] Reference Lab in So. CAL

[Jennifer MacDonald via Histonet]

Thank you to all that responded.  I have a little more information about 
the project. 

The specimens are subdermal implants in mouse.  The specimens are 
cylinders about 5 mm long.  The first student has about 400 specimens and 
there may be more to follow.  The tissue has been fixed in formalin and 
they are currently in the formalin.  The are ready to be processed.  They 
would like H on the tissues.  Turn around time is not critical, but 
they would like it to be done in less than a couple of weeks. 

Local labs are preferred for time and ease of specimen handling reasons. 
They are located in Monrovia.  Shipping out of state or country is not 
feasible. 
Estimated cost and time frame would help.  I can pass your contact 
information on to the facility.

Thank you,
Jennifer MacDonald

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[Histonet] reference lab in So.CAL

[Jennifer MacDonald via Histonet]

I have a colleague looking for a lab that can handle about 400 specimens 
for H staining.  Any recommendations?
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[Histonet] CSH call for abstracts

[Jennifer MacDonald via Histonet]

Hi All,
The California Society for Histotechnology will have its annual symposium 
in Anaheim, CA May 4-6, 2018.  If you are interested in presenting please 
send me your abstract.  Thank you,
Jennifer
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Re: [Histonet] comparison between microtomes leica RM 2125RTS y thermo HM325

[Jennifer MacDonald via Histonet]

The HM325 also has that feature. 



From:   Jay Lundgren via Histonet 
To: Julio Benavides 
Cc: histonet 
Date:   09/01/2017 10:11 AM
Subject:Re: [Histonet] comparison between microtomes leica RM 
2125RTS y thermo HM325



I prefer the Leica, because it has that nifty rough cut button, but that's
just my personal choice.  The Thermo has a better waste tray.  I think
you'd be happy with either one.  The best thing for you to do would be to
get your sales reps to bring them both to your lab to demo and pick your
favorite.

  Jay A. Lundgren, M.S., HTL (ASCP)


On Fri, Sep 1, 2017 at 9:28 AM, Julio Benavides via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

>
> Hi there,
>
> we are buying a new microtome for the lab (research, not high load of
> work) and have been offered leica RM 2125RTS and thermo HM325 with 
similar
> prices.
>
> Could anyone help us to decide?
>
> thanks a lot
>
> cheers
>
> Julio
>
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[Histonet] Digital Microscopes

[Jennifer MacDonald via Histonet]

Is anyone familiar with digital scanning microscopes?  We are in the 
market for one.
Thank you.
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Re: [Histonet] ClearRite

[Jennifer MacDonald via Histonet]

For many years we used ClearRite, both on our tissue processor and for 
staining.  We are now using a similar product from another company.  We 
did not see any problems with our IHC with the use of this product.  We do 
not do molecular testing.  We found it to be far superior to the limonenes 
and much safer to use than xylene.  Coverslipping is another issue.  Not 
all mounting media are compatible with the aliphatic hydrocarbons.  We 
have good success with Permount.
Jennifer MacDonald




From:   "Cartun, Richard via Histonet" <histonet@lists.utsouthwestern.edu>
To: "histonet@lists.utsouthwestern.edu" 
<histonet@lists.utsouthwestern.edu>
Date:   06/09/2017 10:36 AM
Subject:[Histonet] ClearRite



Anyone using ClearRite (xylene replacement) for tissue processing?  If so, 
what's been your experience?  Any effect on IHC or molecular testing? 
Thank you!

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & 
Morphologic Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


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Re: [Histonet] heated block trimmers

[Jennifer MacDonald via Histonet]

We have two of the Paratrimmers by Shandon.  They really cut down on the 
mess with scraping the blocks and the repetitive motion.



From:   "Underwood, Fred via Histonet" 
To: "histonet@lists.utsouthwestern.edu" 

Date:   05/24/2017 06:04 AM
Subject:[Histonet] heated block trimmers



Hi All,

Anyone have any experience with the heated block trimmers?  Specifically, 
I was looking at the one from Newcomer.

Thanks,
Fred
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[Histonet] California Society for Histotechnology

[Jennifer MacDonald via Histonet]

It's not too late to register for the 2017 CSH 40th Annual symposium. Lots 
of great workshops! 
No late fees!

http://californiahistology.org/events.html#csh2017
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[Histonet] CE opportunities

[Jennifer MacDonald via Histonet]

   The California Society for Histotechnology is hosting their annual
   symposium May 18-21 in San Jose.  Please join us for some great
   workshops and California spring weather.  San Jose is a great airport
   to fly into.

   More information can be found at:
   http://californiahistology.org/events.html
   The deadline for CSH hotel pricing is April 28.
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Re: [Histonet] hirsch-peiffer cresyl violet method?

[Jennifer MacDonald via Histonet]

Tim,
I have this method in one of medical technology books from many years ago. 
 Lynch's Medical Laboratory Technology II.  The author of the Histology 
section was Charles F. A. Culling.  There are two procedures, one for 
urine and one for nervous system tissue.  I assume you want the nervous 
system?

Procedure:
1.  Cut 8 to 12 um frozen sections of formalin-fixed tissue into distilled 
water.
2. Stain free-floating sections in 1% cresyl echt violet in 1% acetic acid 
(pH about 2.7) for 10 minutes (pH 3.5 to 3.6 is preferable) as with urine.
3. Rinse in distilled water and mount in glycerin.

Results
Granules of metachromatic leukodystrophy - golden brown that remains 
uncvhanged after treatment with 1% ammonium water.
The granules stain positively with PAS in paraffin sections, but the 
present author has fuond that the PAS-positive reaction faded in routinely 
mounted sections after 6 to 12 months.

I can scan the page for you if you'd like.
Jennifer



From:   "Morken, Timothy via Histonet" 
To: Histonet 
Date:   04/14/2017 11:45 AM
Subject:[Histonet] hirsch-peiffer cresyl violet  method?



Hi all,

Does anyone have the method for Hirsch-peiffer cresyl violet used  for 
metachromatic leukodystrophy? On frozen sections.

We are trying to find out if it is different than the usual cresyl violet 
with acetic acid method for Nissl bodies.

I've found many references to it, but none that give the procedure. And 
the original paper is from 1955 not easily available. And is in German



Thanks for any help.

Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center

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Re: [Histonet] egg albumin

[Jennifer MacDonald via Histonet]

I respectively disagree with this analogy.  Eggnog is dairy based, made 
with milk, heavy cream and sugar.  Dairy products have a much shorter life 
than egg whites.




From:   Rene J Buesa via Histonet 
To: Nancy Schmitt , 
"'histonet@lists.utsouthwestern.edu'" 
Date:   04/12/2017 09:07 AM
Subject:Re: [Histonet] egg albumin



Do you have a bottle of "Eggnog"? Egg-albumin will have similar shelf life 
if refrigerated.René 

On Wednesday, April 12, 2017 7:40 AM, Nancy Schmitt via Histonet 
 wrote:
 

 Good Morning-

Thoughts on shelf life of egg albumin?

Thank you

Nancy Schmitt MLT, HT(ASCP)


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[Histonet] Clinical Laboratory Educators' Conference

[Jennifer MacDonald via Histonet]

This is a great opportunity for those teaching histotechnology.  Although 
many of the workshops are CLS oriented there are many that are about 
teaching laboratory science in general.  There are also workshops about 
clinical rotation issues.
I have gone several years and always bring back great information for our 
program.
Below is a link for the information.


http://www.ascls.org/education-meetings/clec
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[Histonet] California State Histo Symposium

[Jennifer MacDonald via Histonet]

The California Society for Histotechnology will be hosting the 41st Annual 
Symposium in San Jose May 19-21 at the Hilton.  We are accepting abstracts 
for workshops at this time.
Although it is the 41st we will be celebrating our 40th. 

Please email me with your abstract.

jmacdonald...@gmail.com
or
jmacdon...@mtsac.edu
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Re: [Histonet] Congo Red Controls

[Jennifer MacDonald via Histonet]

We cut ours at 8 um within a couple of days of when we are going to use 
them.  We bake them as usual.



From:   "Myles, Irene via Histonet" 
To: "'histonet@lists.utsouthwestern.edu'" 

Date:   11/14/2016 12:26 PM
Subject:[Histonet] Congo Red Controls



Good Afternoon Histonet,

How does everyone handle their Congo Red Controls; thickness, room temp or 
refridgerator, how long do they last once cut, keep them baked or unbaked 
and anything else I did not list.  Thank you very much.

Irene

Irene Myles, HT (ASCP)
Senior Histology Technician
Brigham and Women's Hospital
Histology; Dept. of Pathology
Amory Bldg; 3rd flr; RM 368J
75 Francis Street
Boston, MA 02215
617-732-5454



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Re: [Histonet] Bachelor's degrees to sit for HTL

[Jennifer MacDonald via Histonet]

Fawn,
There is no specification as to what type of degree it must be.  It can be 
any baccalaureate degree as long as you have at least 30 semester hours of 
biology and chemistry combined.  I have had students with art and 
psychology degrees sit for the HTL exam.  They came back to college to get 
the additional science classes to satisfy the HTL (ASCP) requirement.
Jennifer



From:   Fawn Bomar via Histonet 
To: "histonet@lists.utsouthwestern.edu" 

Date:   10/20/2016 08:27 AM
Subject:[Histonet] Bachelor's degrees to sit for HTL



Hi everyone,



I was wondering if anyone would be able to help my co-worker and myself in 
trying to figure out exactly what we need to do to obtain a bachelor's 
degree that would qualify us to sit for the HTL.  As of right now we both 
have associate's degrees and our HT certification.  We both work full time 
and have children so we cannot attend college full time and would have to 
do most of it online if possible due to our location in South Boston, 
Virginia.



We are trying to figure out how to blend online classes with the science 
classes that may have labs required that we would have to attend campus 
for.



Another question we are having is what specific bachelor's degree do we 
have to obtain, does it have to be a biology degree or a science degree?



Thanks for all your help,



Fawn Bomar
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Re: [Histonet] taking the HTL

[Jennifer MacDonald via Histonet]

This may be a better option:  
https://www.labce.com/histology_exam_simulator.aspx
The NSH has partnered with media lab for exams.  You have a full year 
access to the questions.  There are over 2,000 questions in the question 
bank and there are images as well.  NSH members pay the discounted rate of 
$79.  Non NSH members pay $99.

Jennifer



From:   Lauren Sweeney via Histonet 
To: "histonet@lists.utsouthwestern.edu" 

Date:   09/19/2016 10:36 AM
Subject:[Histonet] taking the HTL



Hello all,

I was wondering if anyone out there has used the HTL practice tests 
package you can buy from ASCP to prepare for their exam, and if you did 
was it worth $30?

Thanks!

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[Histonet] Nurses in charge of labs

[Jennifer MacDonald via Histonet]

This decision could affect histology labs as well.  Please read below and 
consider the effects of having a nurse in charge of the lab.
Jennifer

Petition Urging CMS to Reconsider Ruling on Nursing Degree Equivalence

ASCLS, in conjunction with our partners on the Board of Certification, 
urge the laboratory community and other interested individuals to Sign the 
Petition urging the Centers for Medicare and Medicaid Services (CMS) to 
reconsider its position that nursing is a biological science for purposes 
of performing laboratory testing.
[ASCLS_Logo]
Government Affairs Alert


Sign Petition Urging CMS to Reconsider Ruling on Nursing Degree 
Equivalence
ASCLS, in conjunction with our partners on the Board of Certification, 
urge the laboratory community and other interested individuals to Sign the 
Petition urging the Centers for Medicare & Medicaid Services (CMS) to 
reconsider its position that nursing is a biological science for purposes 
of performing laboratory testing.

Sign the petition here.<
http://weblaunch.blifax.com/listener3/redirect?l=8b3397a5-c6e1-4643-a69c-00f119b9c805=ca0c8eaf-ab6f-e611-95c6-0050569f409f=http%3a%2f%2fcqrcengage.com%2fascpath%2fapp%2fsign-petition%3f0%26engagementId%3d239813
>

On April 1, CMS announced that “an associate’s or bachelor’s degree in 
nursing is equivalent to an associate’s or bachelor’s degree, 
respectively, in biological science”—seemingly declaring that individuals 
with a nursing degree are potentially as qualified to perform advanced 
testing as certified laboratory professionals. It also appears that CMS’s 
position could allow individuals with as little as a bachelor’s degree in 
nursing to direct a CLIA moderate complexity laboratory and/or serve in 
senior supervisory roles within a CLIA high complexity laboratory. Since 
the Clinical Laboratory Improvement Amendments (CLIA) of 1988 doesn’t 
specifically require clinical training of individuals with a degree in 
biological sciences, CMS’s new policy exempts individuals with a 
bachelor’s degree in nursing from any specific training requirement prior 
to performing high complexity testing for diagnostic purposes.

We have great respect for the work and invaluable services nurses provide 
patients, but we do not agree that the nursing degree is equivalent to a 
biological sciences degree or that it would adequately prepare someone to 
perform non-waived laboratory services.

Our greatest concern is this policy will negatively affect test quality 
and patient outcomes as well as effect access to quality testing services. 
Take a few minutes to Sign the Petition to tell CMS<
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> that you believe a degree in nursing is not the same thing as a degree 
in the biological sciences and that appropriate academic coursework and 
clinical training/experience are need to provide quality testing services.







If you have any questions, please direct them to ASCLS Executive Vice 
President Jim Flanigan via email at j...@ascls.org.



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Message 

Re: [Histonet] Eosin

[Jennifer MacDonald via Histonet]

Elizabeth,
You can try to lower the pH of the eosin a bit.  Add some acetic acid. Use 
absolute alcohol after the eosin for differentiating/dehydrating.
Jennifer



From:   "Cameron, Elizabeth via Histonet" 

To: "histonet@lists.utsouthwestern.edu" 

Date:   08/30/2016 07:29 AM
Subject:[Histonet] Eosin



Hi,
I have been staining fish tissues fixed in Davidsons with H, and the 
researcher would like the eosin to be more intense.  Our standard protocol 
works well for our own tissue, but the fish look much more washed out.  I 
am using alcoholic eosin Y, have tried both water and alcohol before and I 
have varied the alcohol differentiation steps after the Eosin.  I also 
extended the time in Eosin and increased the wash after bluing to make 
sure the sections are not basic.  Any suggestions would be appreciated.
Thank you.

Elizabeth M. Cameron, HT(ASCP), QIHCCM
Lead Histologist
Mid Coast Hospital
123 Medical Center Drive
Brunswick, ME 04011
(207) 373-6573

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Re: [Histonet] On the hunt for new microtomes!

[Jennifer MacDonald via Histonet]

Your ignorant sarcasm isn't appreciated. Leica makes the microtome and
private labels it for Sakura.

Sent from my iPhone

> On Aug 2, 2016, at 7:46 AM, Rene J Buesa via Histonet
 wrote:
>
> As far as I know Leica Microsystems has bought/absorbed along the years
many optical/instruments makers, such as Baush, American Optical,
Cambridge Instruments, Wild-Heerburg (Switzerland, although this one was a
"reverse" acquisition), Australian "Bond" manufacturers, NOVOCASTRA
laboratories BUT I have never heard that it has bought Sakura instruments.
It would be nice if somebody has reliable information about this alleged
acquisition.René
>
>On Monday, August 1, 2016 4:02 PM, Rene J Buesa via Histonet
 wrote:
>
>
> I don't know now, but some years ago Thermo instruments were less that
reliable. Try Leica or even better Sakura.René
>
> On Monday, August 1, 2016 3:20 PM, Mary Faith Encarnacion via
Histonet  wrote:
>
>
> Hey HistoNet,
>
> Thanks to everyone who helped me out by providing their opinions on
> embedding centers.  This time, I need everyone's thoughts on microtomes.
> My lab is debating between the Thermo/Microm HM355S and the Leica RM2255.
> Your thoughts and advice are very much appreciated!  If there are any
more
> I should try, let me know!
>
> Thanks again,
> Mary Faith
> Histology Supervisor
> VA Palo Alto Health Care System
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Re: [Histonet] On the hunt for new microtomes!

[Jennifer MacDonald via Histonet]

Lecia makes the Sakura microtome.  We have both Sakura and Microm 
microtomes in our student lab.  They have both proved to be reliable. 
There is a difference between a Shandon microtome and a Microm microtome. 



From:   Rene J Buesa via Histonet 
To: Mary Faith Encarnacion , 
"histonet@lists.utsouthwestern.edu" 
Date:   08/01/2016 12:42 PM
Subject:Re: [Histonet] On the hunt for new microtomes!



I don't know now, but some years ago Thermo instruments were less that 
reliable. Try Leica or even better Sakura.René 

On Monday, August 1, 2016 3:20 PM, Mary Faith Encarnacion via Histonet 
 wrote:
 

 Hey HistoNet,

Thanks to everyone who helped me out by providing their opinions on
embedding centers.  This time, I need everyone's thoughts on microtomes.
My lab is debating between the Thermo/Microm HM355S and the Leica RM2255.
Your thoughts and advice are very much appreciated!  If there are any more
I should try, let me know!

Thanks again,
Mary Faith
Histology Supervisor
VA Palo Alto Health Care System
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Re: [Histonet] Reticulin Stain

[Jennifer MacDonald via Histonet]

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Re: [Histonet] block scrapers

[Jennifer MacDonald via Histonet]

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Re: [Histonet] Cleaning Tissue Molds

[Jennifer MacDonald via Histonet]

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Re: [Histonet] Poor Staining

[Jennifer MacDonald via Histonet]

Heat can cause poor chromatin staining.  Is the two shades of eosin only 
on the biopsies, or all tissues? 



From:   Charles Riley via Histonet 
To: histonet@lists.utsouthwestern.edu
Date:   06/16/2016 11:19 AM
Subject:[Histonet] Poor Staining



Can over processing small biopsies lead to poor chromatin staining? Also
can this cause an issue with the eosin staining ( for example only getting
to shades of pink instead of three)?

Please give me any feedback you can

-- 

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs
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Re: [Histonet] warthin-starry stain

[Jennifer MacDonald via Histonet]

are you doing a microwave method or traditional?



From:   Jeff Halstead via Histonet 
To: "histonet@lists.utsouthwestern.edu" 

Date:   06/16/2016 10:17 AM
Subject:[Histonet] warthin-starry stain



Hello histonet---I am a tech in western Oregon, and I have been 
experiencing strange problems with the afore mentioned stain. When the 
stain is run a very strange grey precipitate deposits upon the slides. The 
deposition is not uniform across the slides and varies from slide to slide 
within the run. I use bleach washed slides and all glassware is also 
bleach washed. The slides I am using are non charged with only frosted 
top. The last two runs I used reagents from just opened bottles. No meatal 
contacts the slides or the glassware used to assimilate the reagents. As I 
said---what gives. To say I am perplexed is understating my mood. I have 
even tried washing the slides in the hot water bath to no improvement. My 
path has a list of cases to run and is a bit anxious. Please help. Anyone 
with any ideas ? one step I did not mention-I bleach wash the slides-wash 
in di water-soak in 100% ethanol-then air dry and cover. Also thought I 
might mention I de-wax the pookers from 30 minut
 es to 45 minutes. Thanx for the time   jeff
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Re: [Histonet] Powder Picric Acid vs Liquid Picric Acid

[Jennifer MacDonald via Histonet]

0.5 grams in 400 mL is 0.125%.  Dilute your 1.3% tenfold and it will be 
close enough.



From:   Amy Johnson via Histonet 
To: "histonet@lists.utsouthwestern.edu" 

Date:   06/13/2016 10:47 AM
Subject:[Histonet] Powder Picric Acid vs Liquid Picric Acid



Our current procedure for the Brown and Brenn Gram stain uses 0.5 grams of 
picric acid to 400mls of acetoneis there a way to use a 1.3% 
Picric Acid solution in place of the powdered Picric Acid?

Amylin Johnson, B.S. HTL(ASCP)
Associates in Pathology
Wausau Wi 54401
715-847-2130

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Re: [Histonet] Histonet for Med Techs

[Jennifer MacDonald via Histonet]

clse...@list.apsu.edu
This is a list serve for MT/MLT Educators.

Sent from my iPhone

> On May 23, 2016, at 6:09 AM, Pam Barker via Histonet
 wrote:
>
> Hi Histonetters!
> I hope everyone is looking forward to a great week and a fantastic
Memorial
> Day Weekend.  I am hoping someone can help me with this.
> I would like to know if anyone is aware of a forum similar to histonet
but
> for med techs?  (Besides Medlab out of the University of Buffalo).
>
> Thanks-Pam
>
> Right Place, Right Time, Right Move with RELIA!
>
> Thank You!
>  Pam M. Barker
>
> Pam Barker
> President/Senior Recruiting Specialist-Histology
> RELIA Solutions
> Specialists in Allied Healthcare Recruiting
> 5703 Red Bug Lake Road #330
> Winter Springs, FL 32708-4969
> Phone: (407)657-2027
> Cell: (407)353-5070
> FAX: (407)678-2788
> E-mail: rel...@earthlink.net
> www.facebook.com/PamBarkerRELIA
> www.linkedin.com/in/reliasolutions
> www.twitter.com/pamatrelia
>
>
>
>
>
>
>
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Re: [Histonet] PAS Stain

[Jennifer MacDonald via Histonet]

We switched form malt diastase to alpha amylase and have seen a 
significant improvement in digestion, but as others have said enzyme 
activity will be destroyed at 60 degrees C. We put ours into a 37 degree C 
water bath.  Enzymes work optimally at 37C, body temperature.  As the 
temperature rises above 37C you will see a decrease in enzyme activity and 
then none at all.
Jennifer



From:   Joanne Clark via Histonet 
To: "histonet@lists.utsouthwestern.edu" 

Date:   05/04/2016 01:04 PM
Subject:[Histonet] PAS Stain



Hi Histonetters, I'm hoping someone can help me troubleshoot our PAS 
diastase method.  We have been digesting the tissue in 0.5% diastase of 
malt in a 60 degree oven for 30 minutes, but do not see the glycogen being 
digested out.  I have tried alpha amylase and beta amylase also without 
any luck.  Does anyone have any suggestions to get the digestion to work

Joanne Clark, BAAS, HT(ASCP)CM
Director of Histology

P.   (575) 622-5600
C.   (575) 317-6403
F.   (575) 622-3720
TF. (800) 753-7284

pcnm.com




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Re: [Histonet] Sea urchin Spines

[Jennifer MacDonald via Histonet]

I'm told that it is the ammonia in urine that is the "active ingredient". 
Perhaps trying ammonia water?



From:   "deGuzman, Jose R via Histonet" 

To: "histonet@lists.utsouthwestern.edu" 

Date:   04/27/2016 01:58 PM
Subject:Re: [Histonet] Sea urchin Spines



Have you tried urine or something similar? I know that urine dissolves the 
spine. 



Does anyone have any tips on how to get a section of a sea urchin spine? I 
tried decal but that didn't soften it at all and I tried using nair. If 
anyone has any suggestions please let me know

-- 

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs



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Re: [Histonet] Blog Post Not lab related

[Jennifer MacDonald via Histonet]

In addition to the Histonet, I am on a listserve for clinical laboratory 
educators.  Dr. Raff posts his blog link on that listserve as well.   He 
also responds to some of the questions.  There has never been one public 
complaint regarding his posts.  It is very clear in the subject line and 
the ability to delete his posts are quite easy.  Please don't give 
credence to the myth that the clinical lab has more patience and common 
sense than the histology world. 



From:   Rene J Buesa via Histonet 
To: Caroline Miller , Lester Raff MD 

Cc: "histonet@lists.utsouthwestern.edu" 

Date:   04/14/2016 06:02 AM
Subject:Re: [Histonet] Blog Post Not lab related



Amen!!!But you have to concede that Lester is a very  persistent, almost 
obstinate individual, probably used to impose his will and this postings 
are just an example of it: he likes his blog and tries to impose it to 
everyone. Evidently he has all the time in the world and just does not 
know what to do with it and enjoys sharing his "witty" side. René 

On Wednesday, April 13, 2016 3:24 PM, Caroline Miller via Histonet 
 wrote:
 

 Lester, I do believe this is the second 'non lab related' blog post this
week. As we have spoken about before I do not think histonet is an
appropriate place for your blog posts. I, personally, was totally OK with
you co-posting with other lab related topics. However you are now pushing
it and I respectfully ask that you refrain from sending out your blog 
posts
to the whole list, let people sign up if they are interested.

The issue is that this is a histology related list, if everyone posted
non-lab stuff here it would soon be chaos!

Respectfully yours,
mills

On Wed, Apr 13, 2016 at 11:38 AM, Lester Raff MD via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Just some philosophy towards the end of a long day.
>
>
> 
http://www.chicagonow.com/downsize-maybe/2016/04/kitten-power-can-get-things-done/

>
>
>
> Just a reminder-I try to limit my blog invitations here. If you enjoy 
the
> blog, remember to subscribe (no charge, no spam) on the ChicagoNow blog
> site.
>
> Thanks for your readership.
>
> Lester J. Raff, MD MBA
> UroPartners
> Medical Director Of Laboratory
> 2225 Enterprise Dr. Suite 2511
> Westchester, Il 60154
> Tel: 708-486-0076
> Fax: 708-492-0203
>
> ___
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>



-- 
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297
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Re: [Histonet] Reference for erythrosin-eosin counterstain for H

[Jennifer MacDonald via Histonet]

There is a reference to erythrosin in Lynch's Medical Laboratory 
Technology, but it is brief.  Culling is the author of the histology 
chapter, so perhaps he has something more in depth.



From:   "Tony Henwood (SCHN) via Histonet" 

To: "histonet@lists.utsouthwestern.edu" 

Date:   04/03/2016 05:20 PM
Subject:[Histonet] Reference for erythrosin-eosin counterstain for 
H



Hi all,

I am doing an H workshop next month and one of the Hx counterstains I 
will be including is Erythrosin-eosin (see below).
This counterstain is the preferred by some of our major Histopathology 
departments in Australia.

The issue I have is that I cannot find a reference for this variant of the 
H

Anyone have any ideas?


Eosin Y (CI 45380) 5g
Erythrosin B (CI 45430)   5g
Sodium Hydrogen Carbonate 1.25g
Magnesium Sulphate 10g
Distilled water   500ml
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principle Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA


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Re: [Histonet] Nuclear Bubbling

[Jennifer MacDonald via Histonet]

There is no need to be rude.  He has tried the drying option and is still 
having nuclear bubbling.  He is exploring other possible issues.  You 
would see this if you read the email in its entirety. 



From:   Rene J Buesa via Histonet 
To: "Vickroy, James" , 
"histonet@lists.utsouthwestern.edu" 
Date:   02/16/2016 10:13 AM
Subject:Re: [Histonet] Nuclear Bubbling



If I remember correctly, this issue has been discussed previously.The 
general consensus as to the cause of nuclear "bubbling" (in reality a lack 
of staining in the nuclear area) has been attributed to an incomplete 
section drying.After the section has be "fished" from the water bath, if 
the slide is not set to drain the underneath water before drying, the 
nuclear components are dissolved hence when the section is stained, there 
is nothing to stain → "nuclear bubbling".I think this has been previously 
stated so I really do not understand posting this same question again.I do 
not think that posting again the question a different answer is going to 
be received.rené 

On Tuesday, February 16, 2016 12:32 PM, "Vickroy, James via Histonet" 
 wrote:
 

 
Struggling to find an answer.  We do a lot of GI biopsies in our lab.  
Sometimes they look wonderful without any nuclear bubbling, other times 
the bubbling is pretty intense.  Since nuclear bubbling is often 
attributed to incomplete fixation we of course have investigated the 
fixation times.  I do not find that the problem is fixation.  In fact some 
of the biopsies end up fixing for 48 hrs before processing. (weekend).  
There was a suggestion last week or so that there might be water trapped 
under the slides after cutting and before staining.  I really thought that 
this might be the issue however I'm not sure at this point.  Extra drying 
seems to help but sometimes slides side by side are so variable, one with 
bubbles and one without.  I also don't believe the problem is in the 
processing schedule since the problem has shown up on both a rapid and a 
normal schedule. (therefore longer dehydration, clearing, etc.)

I am wondering if anyone else has worked with this issue.  Here are my 
questions:


1.Could it be something that is happening with the tissue before 
it gets to the lab?  Usually a delay if fixation  causes other artifacts 
but not bubbling.  Could it be heat from the GI procedure?

2.  We do use blue sponges for our biopsies.  I know some say get rid 
of the sponges but has anyone seen this problem caused by usage of 
sponges?

3.  What about the heat stage in our Prisma stainer?


I am really getting frustrated.  Pathologists never complain however I 
would rather all of the tissue did not have the "nuclear bubbling".  Again 
we only do biopsies so I really don't think the standard old " not enough 
time in formalin" is the issue.  I have even wondered about variables such 
as we use recycled formalin, recycled Clearite III.

Any suggestions?

Jim



Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvick...@springfieldclinic.com<
mailto:jvick...@springfieldclinic.com>



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Re: [Histonet] unsolved proplem

[Jennifer MacDonald via Histonet]

There are a couple of things that it might be. 
1.   Uneven deparaffinization before staining.
2.  Water in your last reagents/paraffin on the processor.



From:   mohamed abd el razik via Histonet 

To: "Histonet@lists.utsouthwestern.edu" 

Date:   01/26/2016 11:33 AM
Subject:[Histonet] unsolved proplem



Dear allI have submitted a proplem about reprocessing tissue blocks that 
have patches of stained areas and unstained pale yellow areas (H 
stain)!!!, unfortently the proplem didn't solved yet and i have tried all 
possible solutions, increasing processing time and ordered new chemicals 
from other soruces and changed the paraplast company but still have the 
proplem  
i'm using 70% alc 2hr - 80% alc 1hr - 90% alc 45mins- absolut 1 and 2 each 
for 30 mins then Benzen 1 and 2 each for 25 mins then clearing by methyl 
benzoat 2hs at least then paraffin1+2 (Maccormick melting degree 
56-58)each 90 mins for mice kidney, liver and tests
the previous protocol was very satisfactory to us and we have tried to 
stain older sections processed befor the emerging proplem and have a nice 
stain by current H stain to ensure that the proplem is out our staining 
step. 
any suggestions please !!
Mohamed


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Re: [Histonet] Processing of breast tissue

[Jennifer MacDonald via Histonet]

Do you using "freezing spray" on these specimens?  Direct spray can cause 
the tissue to appear burnt. 



From:   Charles Riley via Histonet 
To: histonet@lists.utsouthwestern.edu
Date:   01/05/2016 06:00 AM
Subject:[Histonet] Processing of breast tissue



We have been experiencing some issue lately with cutting our breast
excisions. Recently we had two specimen where after our 8 hr run the 
tissue
was still extremely soft. Our medical director said the fat sectioned
beautifully but the fibrous tissue appeared cooked. Can anyone give a
suggestion as to how we should process our breast excisions from grossing
through to microtomy sectioning.

Currently I have tried to get grossing to limit the specimen to 2mm by 2mm
by 1mm thick. We run an 8hr process using the Thermo Shandon Excelsior
processor. We cut our sections at 4um but have recently had to do them at
6um in order to get the soft samples cut.
-- 

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs

Doctors Pathology Services, Dover DE
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[Histonet] Leica rep in SoCal

[Jennifer MacDonald via Histonet]

Looking for the contact information for Leica sales in Southern 
California.
Thanks,
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Re: [Histonet] Hourly wage for trainee in CA

[Jennifer MacDonald via Histonet]

Akemi,
I think this offer is low for a certified HT/HTL, particularly in that 
area.  I have had graduates go up to Northern CA and the minimum starting 
wage that they have accepted is $25.  These are brand new graduates, with 
minimal experience, but I don't believe their titles are "trainees".
Jennifer



From:   Eileen Akemi Allison via Histonet 

To: Histonet 
Date:   11/28/2015 08:08 AM
Subject:[Histonet] Hourly wage for trainee in CA



Hello all my histology friends in California: 
1st I hope you all had a wonderful Thanksgiving! I would like to know what 
the hourly rate of a new histologist with a HT certification is making. I 
would very much appreciate this information.  We are offering a training 
position to a local individual who doesn't have a degree, HT or HTL 
certification, or recent experience (last worked in histology in 1991). I 
want to see if our offer of $20.00 an hour plus benefit's was out of line. 
 I would prefer to have a certified HT, but no one has answered our ads.

Best regards,

Akemi Allison BS, HT/HTL (ASCP)
Pathology Manager
Monterey Bay GI Consultants Laboratory
23 Upper Ragsdale Drive, Suite 200
Monterey, CA 93940
W: Email: aalli...@montereygi.com 
H: Email: akemiat3...@gmail.com 
Tele: (831) 375-3577 X117

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Re: [Histonet] IHC hand staining

[Jennifer MacDonald via Histonet]

In one of our classes the students do IHC staining by hand.  They perform 
multiple procedures over the semester.  We currently use Biocare reagents. 
 The polymer based detection systems are simple to use.  The data sheets 
that accompany the antibodies and detection reagents are really helpful 
with protocols. 



From:   Elaine allison Hoffman via Histonet 

To: Histonet List 
Date:   11/05/2015 08:08 AM
Subject:[Histonet] IHC hand staining



Greetings Histonetters,
I need some information on "hand" immuno-staining.  We are a small GI lab 
doing Giemsa stains on H. Pylori right now, but the Lab Director wants me 
to look into IHC hand staining for H.Pylori.  Anybody out there doing hand 
staining and willing to give me a protocol to try? Or maybe point me in 
the right direction to who could help me with that.  Any reliable IHC 
staining kits recommended?  Thanks in advance for the help
Elaine Hoffman
The Gastroenterology Clinic & Endoscopy Center, IncGI Pathology 
Laboratory1622 E Market StreetWarren, Ohio   44483

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Re: [Histonet] Pas Digestion

[Jennifer MacDonald via Histonet]

The optimal temperature for most enzymes in 37 degrees Celsius. 




From:   "Lager, Loree via Histonet" 
To: "'histonet@lists.utsouthwestern.edu'" 

Date:   11/03/2015 12:38 PM
Subject:[Histonet] Pas Digestion



Hello,

This is my first time using the histonet, as our veteran user retired.

We've been having an intermittent problem with incomplete glycogen 
digestion on PASD.  Our digestion solution is 0.5%  α-Amylase, Type VI-B 
from porcine pancreas (Sigma), which we freeze in small aliquots and thaw 
for 45 minutes at room temperature before use. We digest for 20 minutes @ 
room temperature, dropping amylase on slides.

In troubleshooting the issue, we discovered amylase was several years old, 
so new amylase ordered.   The new lot (while the same product number) 
produced even worse results.  After a closer look we saw that the new lot 
contained ½ of the units/mg solid, 26 to 13, not sure why?

Anyone willing to share their procedure or suggestions to help us resolve 
this issue?

Thank you,
Loree Lager
Seattle Children's Hospital
Histology




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Re: [Histonet] Coverslipping mystery

[Jennifer MacDonald]

What type of clearing agent are you using?  The aliphatic hydrocarbons are 
not compatible with all mounting media.



From:   Adam Boanas a.boa...@epistem.co.uk
To: histonet@lists.utsouthwestern.edu 
histonet@lists.utsouthwestern.edu
Date:   07/09/2015 06:16 AM
Subject:[Histonet] Coverslipping mystery



Hello,

We are having a problem that is developing into a big issue in our lab and 
I was wondering if anybody could shed any light on it. Our CV5000 
coverslipper has recently started introducing microscopic air bubbles onto 
the slides during coverslipping. We have been told by our engineer that it 
is a consequence of the age and use of the motor and that sourcing another 
for an instrument that old (15yrs) will be v difficult. As such, we have 
been forced to manually coverslip using DPX and a pipette - manually 
applying the coverslips to the slide, thus mirroring the action of the 
coverslipper. This is fine at first and for the next few days the slides 
look great and very clean. However, after about day 4 -5 days post 
coverslipping, the slides develop an odd appearance down the microscope 
which looks like very fine `parched earth / crazy paving` all over the 
slide - including the section. The excess mountant around the edge of the 
coverslip also has a very faint, cloudy appearance whe
 n this occurs. This of course renders the slide un-useable. Does anyone 
have a clue what this might be down to / how we can stop it?
We are struggling for ideas with this one!  - this occurs with fresh DPX 
also.

Many thanks
Adam

Adam Boanas
Senior Research Associate
Epistem Ltd
48 Grafton Street
Manchester, M13 9XX

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Re: [Histonet] General Genetics (lecture and lab textbooks)

[Jennifer MacDonald]

I believe he is trying to find a textbook that others have used and cover 
his questions.  There are many textbooks out there and it is time 
consuming to review all of them, so he is asking if anyone is familiar 
with one that covers his needs.



From:   John Kiernan jkier...@uwo.ca
To: Jorge A. Santiago-Blay blayjo...@gmail.com
Cc: histonet@lists.utsouthwestern.edu 
histonet@lists.utsouthwestern.edu
Date:   06/11/2015 10:33 PM
Subject:Re: [Histonet] General Genetics (lecture and lab 
textbooks)



Dear Jorge A. Santiago-Blay,

How did you learn enough about General Genetics for science majors to be 
hired to teach the subject? Do you not own at least one textbook in the 
field? How did you qualify to teach the subject? 

With the internet (Google) it will be easy to find the most recent edition 
of the text from which you studied General Genetics. You could buy the 
book or ask the publisher for a free copy. Publishers happily provide free 
textbooks to instructors, hoping that every student will buy a copy of the 
adopted textbook. 

Why ask me? My fields of interest, before I retired, were Neuroanatomy and 
Histochemistry, not Genetics.

Does your institution have a way to warn students that an instructor does 
not know a suitable textbook for his course? Your students may need such a 
warning!

J. A. Kiernan
Anatomy  Cell Biology
University of Western Ontario
London, Canada
= = =
On 08/06/15, Jorge A. Santiago-Blay  blayjo...@gmail.com wrote:
 General Genetics (lecture and lab textbooks)
 
 Dear Histonet-Listers:
 
 I wish to know if you have recommendations of *excellent* textbooks
 (lecture and labs) for General Genetics for science majors. Although I 
just
 finished teaching an upper division Genetic Analysis course and I am 
well
 familiarized with what is available, I am not as familiarized with la 
creme
 de la creme for General Genetics.
 
 Ideally, I want a textbook that is (list is not comprehensive):
 1. As inexpensive as possible (available online, softbound, etc.).
 
 2. Easy to read, understand. and profusely illustrated.
 
 3. Does not assume more than intro. biology (for majors) knowledge.
 
 4. Emphasizes principles of genetics, model organisms, critical 
thinking,
 experimental design, ecological and conservation genetics, etc.
 
 5. Has a generous companion packages (incl. PowerPoints, online quiz
 cartridges - not the ones that reside with the publishers but the ones 
than
 reside on campus only, etc.). For anything online, technology is as 
close
 to 100% reliable and intuitive as possible.
 
 6. Does not shy away from math and stats (because I do not shy away from
 those either).
 
 7. Is not divorced of the historical context of genetics.
 
 8. Highlights the importance of genetics in modern life.
 
 9. For labs, all of the above and not just the usual genetics exercises. 
I
 want a book that invites exploration and engages all of us in the field.
 Ideally, with a manual for s/he that may be helping with setting up the 
lab.
 
 If you have any feedback, please kindly send it directly to me,
 blayjo...@gmail.com
 
 With gratefulness,
 
 Jorge
 
 P.S Apologies for potential duplicate emails.
 
 Jorge A. Santiago-Blay, PhD
 blaypublishers.comblay.cfm
 http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm
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Re: [Histonet] OJT Histotechs/Training

[Jennifer MacDonald]

This is an issue with our program as well.  We have a difficult time 
finding clinical sites for our students.  Many people want to hire trained 
individuals, but don't want to invest any time in the training.  Our 
students receive a great deal of hands-on time in the student laboratory, 
but need real life experience. 
Jennifer MacDonald
Mt. San Antonio College



From:   Mayer,Toysha N tnma...@mdanderson.org
To: 'histonet@lists.utsouthwestern.edu' 
histonet@lists.utsouthwestern.edu
Date:   05/14/2015 01:48 PM
Subject:Re: [Histonet] OJT Histotechs/Training



One good way to find techs is to offer to become a clinical affiliate for 
a program.  Most programs struggle with attracting students and providing 
them with clinical affiliates to fine tune their skills.
It may not matter that the school is not located near you, the student may 
have family nearby to stay with. 
We are always looking for long distance affiliates, that way we can 
attract an out-of-state student and not saturate the local area.  I have 
students who want to relocate to different areas and just for a change and 
this helps them do so.  We also get calls from applicants who don't mind 
moving to us for 9-10 months, as long as they can go home when they 
finish. 
If the program is agreeable to this, the specifics can be worked out, such 
as what skills are entry level and the length of the time the student is 
at your facility.
Ours is called an Internship and the student is at the facility for 12 
weeks.  They come in knowing basic embedding, cutting, routine staining, 
specials, and have performed a minimum of three IHC stains.  Two are 
manual and one automated. 
Some programs keep the students in house for some time before they leave 
for internship, while others leave the technical training to the clinics. 
It all depends on what is available. 
This would be a low cost way to see if you like a person, can train them 
and are willing to teach. 
Some students are looking to relocate just before graduation, so a move 
for an internship is a consideration. 
Many times it is the expectations of the trainer that are not aligned with 
the skill level of entry-level techs and that can cause problems.  This 
way the person can come in with an assessment of the skill level and the 
OJT phase can begin.  If the affiliate chooses to hire the student, great. 
 If not, then no harm.  At least you get to say that you tried and did not 
have to waste money doing so.  It is not a source of free labor, but a way 
of accurately assessing a person's fit for your needs.
Many allied health programs (not just histo) are doing this and it helps 
to showcase different labs and programs.

Just my two cents.

Sincerely,

Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP)
Instructor/Education Coordinator
Program in Histotechnology
School of Health Professions
UT M.D. Anderson Cancer Center
713.563-3481





Message: 1
Date: Thu, 14 May 2015 17:07:06 +
From: Morken, Timothy timothy.mor...@ucsf.edu
To: Pam Marcum mucra...@comcast.net, Lisa Roy ro...@labcorp.com
Cc: Histonet histonet@lists.utsouthwestern.edu, Michael Dessoye
 mjdess...@commonwealthhealth.net
Subject: Re: [Histonet] OJT Histotechs/Training
Message-ID:
 761e2b5697f795489c8710bcc72141ff36831...@ex07.net.ucsf.edu
Content-Type: text/plain; charset=utf-8

I think there is some actor from the CSI series that has done some of this 
work promoting lab techs...

Tim Morken

-Original Message-
From: Pam Marcum [mailto:mucra...@comcast.net] 
Sent: Thursday, May 14, 2015 9:18 AM
To: Lisa Roy
Cc: Histonet; Michael Dessoye
Subject: Re: [Histonet] OJT Histotechs/Training

I understand and agree with everything being said and feel we do need more 
education in getting your registry, as Histology is changing and 
growing.??We need to be prepared to grow with it, much as we did when IHC 
first came into Histology and many thought it would go to the MTs.?? 
? 
The one thing that has not changed in the 50 years I have done Histology 
is the fact that no one outside of AP knows what a Histologist is or what 
we do.? (I'm tried of being asked Oh what kind of history is that?)? 
Until we change that and get more information about the field and 
advantages we will still be in the straights we are in now.? No one 
joining because so few people even know what we do or that there is an 
opportunity here.? If you don't know what Histology is why would you even 
look at the field.? I know about and have done school visits, career days 
etc; and those are not enough.? 
? 
We need a spokesperson or celebrity?who has needed our services and not 
even known we, Histology, were the ones who did the slides their wonderful 
doctors used to save their lives.? This person or persons needs to speak 
loud and strong the way Robin Roberts has done on TV for her doctors 
and?help.?However; Histology was neven mentioned in those gratis 
moments.?I have only known one?person in NSH who suggested

[Histonet] HT jobs in Eureka, CA

[Jennifer MacDonald]

I have a graduate looking for histotechnician jobs in the Eureka, CA area. 
 Please contact me if you know of any.  Thanks,
Jennifer MacDonald
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RE: [Histonet] RE: Nuclear Artifact

[Jennifer MacDonald]

Our pathologists also complained about some of the GI biopsies looking 
burnt.  We tracked all of the problem cases back to one histotech.  The 
histotech was causing the burn artifact with excessive use of freezing 
spray. 



From:   Arbaugh, Roberta rarba...@csdermatology.com
To: 'Morken, Timothy' timothy.mor...@ucsf.edu, Sue 
suetp...@comcast.net, Lisa Roy ro...@labcorp.com
Cc: histonet@lists.utsouthwestern.edu 
histonet@lists.utsouthwestern.edu
Date:   04/22/2015 11:32 AM
Subject:RE: [Histonet] RE: Nuclear Artifact
Sent by:histonet-boun...@lists.utsouthwestern.edu



We have had the same problem. We come to the conclusion that it was water 
droplets. We had the problem when our humidity was high in the lab, or our 
weekend run. The changes we made where :
1. We no longer process over the weekend . We cannot count on our heating 
and cooling in our building.
2. We place a towel and a thick piece of cardboard on top of the processor 
lid.
3.We do not use the really fine mesh cassettes. We will use formalin 
soaked sponges or perm papers.
4. We do not over pack cassette basket.
We had our processor check every time we saw the artifact and they could 
never find a problem with the processor.
Hope this help, Roberta

-Original Message-
From: Morken, Timothy [mailto:timothy.mor...@ucsf.edu]
Sent: Wednesday, April 22, 2015 11:16 AM
To: Sue; Lisa Roy
Cc: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Nuclear Artifact

One pathologist said it looks like the tissue has been cooked.  Which 
could also be drying artifact after bx, before formalin.

The only issue is we can have two biopsies right next to one another in 
the basket one looks good and one looks bad. My director also thinks it is 
the processors.

Same processor but two results? Solving intermittent problems takes time 
to check variables - time almost no one wants to spend to check out every 
possibility. But if one variable is the same for both, and the result is 
different, then most likely it is a different variable causing the 
problem.

I think the other idea suggested of checking the handling of the tissue at 
the source of the biopsy is more likely to shed some light on this issue.


Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department 
of Pathology UC San Francisco Medical Center





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu [
mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue
Sent: Tuesday, April 21, 2015 4:55 PM
To: Lisa Roy
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Nuclear Artifact

OMG we are experiencing the same issue. At first it was just GI and now we 
are seeing it on prostate. One pathologist said it looks like the tissue 
has been cooked. The only issue is we can have two biopsies right next to 
one another in the basket one looks good and one looks bad. My director 
also thinks it is the processors. I had Thermo out and they could find 
nothing. We changed out all the reagents and the biopsies were fine than 
two days later we had some bad ones. I know in July Fisher had a formalin 
recall associated to the mixture of buffer, water and formalin. We thought 
that might be it but it is now almost a year later and all the bad 
formalin should be gone. The histotechs say the tissue is crunchy and they 
are right. I am running a test tonight of a small needle biopsy that I 
made from a colon. I placed it is straight formaldehyde overnight and am 
processing it on our biopsy cycle tonight. My director also wanted us to 
only put three levels on our Thermo, but he wanted the middle level to 
have empty baskets. I stopped that today because I think the other issue 
is that the poor biopsies may be on the top level and as the reagents are 
used the level changes, and also due to displacement with the middle level 
being empty the reagent levels may not reach the top. We just do not have 
the manpower to inspect every reagent every day, we have 6 processor and 
it would take a tech all day. We actually take a digital picture when they 
come out of the processor. I want to check my problems cases tomorrow. We 
do not use sponges but the only other like was the PA who was wrapping the 
blue paper very tight around the tissue. I really do not think this is the 
issue though.. Any other insight would be greatly appreciated.

Susan T. Paturzo
TJUH
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[Histonet] Histo jobs in the Eureka area

[Jennifer MacDonald]

I have a student that will graduate in June with eligibility to sit for the
HT exam. She will be looking for a job in the Eureka area. Does anyone know
about the HT employment outlook there?
Thank you,
Jennifer MacDonald

Sent from my iPhone


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[Histonet] CSH Hotel deadline

[Jennifer MacDonald]

CSH rate at the hotel ends tonight.  If you plan on attending the CSH 
please make your hotel reservations.  Lots of great workshops and 
beautiful location.

http://californiahistology.org/events.html
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Re: [Histonet] Reference needed

[Jennifer MacDonald]

No.  Someone in another lab asked me if I knew if there was documentation 
of how these work.  I did not have this in my resources.  I will look for 
the book you mentioned.  It is more curiosity than need.  I am also 
curious of the chemistry.  Thank you.
Jennifer



From:   John Kiernan jkier...@uwo.ca
To: Jennifer MacDonald jmacdon...@mtsac.edu, 
histonet@lists.utsouthwestern.edu
Date:   04/13/2015 10:23 PM
Subject:Re: [Histonet] Reference needed



Do you need a source to satisfy ignorant officials? 
Try a good textbook that shows up in second-hand bookshops. 
Brown,GC 1978 An Introduction to Histotechnology. New York: 
Appleton-Century-Crofts. 
It doesn't seem to have an ISBN.
John Kiernan
= = =
On 13/04/15, Jennifer MacDonald jmacdon...@mtsac.edu wrote:
Does anyone have a reference for the theory of:
1.  The use of soapy water to prevent fatty tissue from blowing 
apart in the flotation bath?
2.  The use of ammonia water to rehydrate tissues?
Many of us use these tricks, but is there a source for the theory?
Thanks,
Jennifer MacDonald
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Re: [Histonet] Schiffs

[Jennifer MacDonald]

Schiff into 10 mL of 37-40% formaldehyde



From:   Bernice Frederick b-freder...@northwestern.edu
To: Histonet@lists.utsouthwestern.edu 
histonet@lists.utsouthwestern.edu
Date:   04/13/2015 12:40 PM
Subject:[Histonet] Schiffs
Sent by:histonet-boun...@lists.utsouthwestern.edu



Having a brain fart all- does the Schiff go into the formaldehyde or vice 
versa to test if the Schiffs is still good?
Bernice


Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-freder...@northwestern.edumailto:b-freder...@northwestern.edu

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[Histonet] Reference needed

[Jennifer MacDonald]

Does anyone have a reference for the theory of:
1.  The use of soapy water to prevent fatty tissue from blowing 
apart in the flotation bath?
2.  The use of ammonia water to rehydrate tissues?
Many of us use these tricks, but is there a source for the theory?
Thanks,
Jennifer MacDonald
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Re: [Histonet] Bile Stain

[Jennifer MacDonald]

Yes this possible.

Sent from my iPhone

 On Apr 8, 2015, at 1:54 PM, Fran Pearsall fpea...@clemson.edu wrote:

 I would like to ask if it's possible to do a Fouchet's reaction in the
 first part of the stain; then instead of doing the Van Giesens as the
 counter stain can I just use Nuclear Fast Red? For some reason my Steins
 Iodine stain isn't getting the bile green? Thank you for any suggestions.



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[Histonet] CSH Symposium

[Jennifer MacDonald]

Just a reminder that the annual CSH Symposium is just around the corner. 
We will be in Santa Cruz/Scotts Valley May 1-3.  Please join us for some 
great workshops. CE documentation will be provided at the completion of 
each workshop for pre-registered attendees.  If you'd like a PDF version 
please email me.

http://californiahistology.org/events.html

Thank you,
Jennifer MacDonald
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Re: [Histonet] Tol Blue

[Jennifer MacDonald]

Mast cells granules are metachromatic.  What you see is the expected 
staining reaction.



From:   Kimberly Marshall kimbe...@animalreferencepathology.com
To: histonet@lists.utsouthwestern.edu 
histonet@lists.utsouthwestern.edu
Date:   03/24/2015 02:20 PM
Subject:[Histonet] Tol Blue
Sent by:histonet-boun...@lists.utsouthwestern.edu



?Hello my fellow Histo Techs.  Have a question I just know someone out 
there can answer for me.

In canine tissue, we are having problems with the Tol Blue for mast cell. 
Am experiencing metachromasia, or the mast cells turning purple not blue. 
I have attempted to decrease time, or add time, but its not helping.  My 
pathologist says he has had this issue before.  So question is.  Could it 
be the mast cell in a dog does not stain the same? Is there another stain 
that may work?  Any help will be much appreciated.


Thanks in Advance.

Kimberly Marshall H.T.(ASCP)






Kimberly Marshall H.T.(ASCP)

Histology/Lab Supervisor

Toll Free 1-800-426-2099

Fax 801-584-5104

PO Box 17580

Salt Lake City, Utah 84107

www.animalreferencepathology.comhttp://www.animalreferencepathology.com/



Advancing the art and science of veterinary medicine



[cid:image001.jpg@01CF8F87.A0BD4830]

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[Histonet] BS in Histotechnology

[Jennifer MacDonald]

In what areas would a facility hire an HTL over an HT?  Is there a need 
for more HTL programs?  4
Thank you,
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Re: [Histonet] Guinea Pig Inner Ear

[Jennifer MacDonald]

are you just looking at the cochlea? 



From:   Michelle Aono aono...@auburn.edu
To: 'histonet@lists.utsouthwestern.edu' 
histonet@lists.utsouthwestern.edu
Date:   03/12/2015 11:40 AM
Subject:[Histonet] Guinea Pig Inner Ear
Sent by:histonet-boun...@lists.utsouthwestern.edu



Hello Histonet!  I am renewing my search for guinea pig inner ear slides 
to replace the old Turtox ones for our histo class.   Does anyone know 
where I might acquire guinea pig inner ear slides?  Or if I could get them 
as fixed, decalcified, embedded blocks, ready for sectioning?  Or if you 
have guinea pig inner ears I could have...  If you have ever sectioned 
inner ear I would love to hear from you!  Orientation seems to be critical 
and the one attempt we did on dog IE was a miserable failure.   Thanks for 
any and all help!

Michelle (Shelly) Aono
~~~
Research Associate II
107B/124 Greene Hall
Auburn University, Dept of APP
Auburn, AL 36849
(334) 844-5594

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Re: [Histonet] PA

[Jennifer MacDonald]

To be a PA you would need to have ASCP certification.  There is one route 
to be eligible for certification.

To be eligible for this examination category, an applicant must have a 
minimum of a baccalaureate degree from a regionally accredited 
college/university, AND successful completion of a NAACLS accredited 
Pathologists’ Assistant program within the last five years.

Most of the NAACLS programs are Masters degrees.



From:   Mike mikeykk...@live.com
To: Histonet@lists.utsouthwestern.edu 
Histonet@lists.utsouthwestern.edu
Date:   03/10/2015 08:49 PM
Subject:[Histonet] PA
Sent by:histonet-boun...@lists.utsouthwestern.edu



Can you be a pathology assistant with on the job training only? Does it 
matter if you have a bachelors vs a masters degree in a science not 
related to pathology or a tech license vs no tech license?
Thanks 
Sent from my iPhone
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[Histonet] CSH Symposium

[Jennifer MacDonald]

The California Society for Histotechnology will be hosting their 39th 
Annual Symposium at the Hilton Scotts Valley/Santa Cruz May 1-3.  The link 
below will take you to our website.  CE certificates will be issued at the 
completion of each workshop for those that have pre-registered for the 
event.  The program can also be emailed to you if you contact me.
Jennifer

http://www.californiahistology.org/events.html
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[Histonet] needle size

[Jennifer MacDonald]

Does anyone have the gauge of needle used for breast core biopsies and for 
prostate biopsies?
Thanks,
Jennifer
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Re: [Histonet] FW: Iron control tissue or blocks

[Jennifer MacDonald]

Spleen is a good, sensitive control to use for iron. 



From:   Montana, Maria mtmont...@primecare.org
To: histonet@lists.utsouthwestern.edu 
histonet@lists.utsouthwestern.edu
Date:   01/20/2015 08:39 AM
Subject:[Histonet] FW: Iron control tissue or blocks
Sent by:histonet-boun...@lists.utsouthwestern.edu






From: Montana, Maria
Sent: Wednesday, January 07, 2015 1:50 PM
To: hiso...@lists.utsouthwestern.edu
Subject: Iron control tissue or blocks

Hi, Histoneters,

I was wondering if anyone out there has some good Fe control tissue or 
blocks that you would be willing to trade for. We have Amyloid tissue, if 
you are interested.

Thanks,

Maria Montana HTL (ASCP)
CHI St Alexius Health
(701)530-6732



This email may include confidential and privileged information. If this is 
not intended for your use, please destroy immediately and contact the 
sender of the message.





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Re: [Histonet] Cheap Disposable Blades for Facing In

[Jennifer MacDonald]

We save our blade from the previous day to use for facing. We keep them in
slide mailers.

Sent from my iPad

 On Jan 22, 2015, at 4:21 PM, Sandra Cheasty
cheas...@svm.vetmed.wisc.edu wrote:

 Hello everyone,

 Does anyone have a source for cheap, low-profile blades
for facing in blocks?

 Thanks!
 Sandy



 Sandra J. Cheasty, HT (ASCP)

 Histology  Necropsy Supervisor, President Keith Richards Fan Club

 UW-Madison, School of Veterinary Medicine









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[Histonet] PAS rx

[Jennifer MacDonald]

Some PAS procedures call for sulphurous rinses after the Schiffs reagent. 
From your experience is there a noticeable difference if you do not do the 
rinse?  Would it matter if you used sodium metabisulfite rather than 
potassium metabisulfite? 
Thank,
Jennifer
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Re: And other crazy stuff. RE: [Histonet] cutting honey bees

[Jennifer MacDonald]

Termites for a science fair project.
anchovy from a pizza to pull a joke on a pathologist
chicken bone from dim sum chicken feet, again to pull one over on a 
pathologist.  Not decalcified, embedded in resin.



From:   Morken, Timothy timothy.mor...@ucsf.edu
To: Patsy Ruegg prueg...@hotmail.com, Roberta Horner r...@psu.edu, 
Douglas Gregg classic...@gmail.com, Histonet@Lists. Edu 
histonet@lists.utsouthwestern.edu
Date:   01/08/2015 12:00 PM
Subject:And other crazy stuff.  RE: [Histonet] cutting honey bees
Sent by:histonet-boun...@lists.utsouthwestern.edu



You crazy research people...OK, so what is the craziest thing you ever had 
to cut, or were asked to cut?

For me, not too bad, but embedding for EM and sectioning a single oocyte 
that was nearly microscopic. I'll just say it took a LOT of thick sections 
too face down to it without actually cutting through it.


Open the floodgates

Tim Morken

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu [
mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Tuesday, January 06, 2015 11:13 AM
To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu
Subject: RE: [Histonet] cutting honey bees

for the whole bee I probably would process and embed it in glycol 
methacrylate (gma) it is much harder and would give better sections, we 
have done zebra fish and several other harder tissues including calcified 
bone in GMA.

Cheers,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



 From: r...@psu.edu
 To: classic...@gmail.com; histonet@lists.utsouthwestern.edu
 Date: Sat, 3 Jan 2015 23:15:33 +
 Subject: RE: [Histonet] cutting honey bees
 CC: 
 
 I sectioned and stained honey bee and yellow jacket stingers years ago. 
They wanted to show the difference between the stingers.  I wasn't sure 
what to do so I processed and handled like everything else.  I was able to 
get some good sections.  I put 6 stingers in each block and cut several 
sections figuring there should be at least one good stinger in each block 
and it worked.
 Roberta Horner
 Penn State University
 Animal Diagnostic Lab
 
 From: histonet-boun...@lists.utsouthwestern.edu 
 [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg 
 [classic...@gmail.com]
 Sent: Saturday, January 03, 2015 6:08 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] cutting honey bees
 
 Has anyone had experience embedding and cutting honey bees. I am sure 
 there are some issues with the harder exoskeleton. Would that have to 
 be dissected away first. I am considering helping a student with a 
 science fair project on bees.
 
 Douglas Gregg
 Veterianary pathologist
 
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[Histonet] plant processing

[Jennifer MacDonald]

I have a colleague at a neighboring school that would like to process 
plant tissue for materials for his botany class.  Is there a good resource 
for plant processing?  Is there anyone in southern California that is 
processing plant materials?
Thank you,
Jennifer
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[Histonet] CSH 2015

[Jennifer MacDonald]

We are looking for workshops for our 2015 meeting.  Does anyone have a 
MOHS workshop that they would like to present?  A workshop on grossing?
Thanks,
Jennifer MacDonald
CSH Education Chair
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RE: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek

[Jennifer MacDonald]

There may be a leaking valve. 



From:   Jamal j.rowa...@alborglaboratories.com
To: 'Arun Jyothi S.P' arunjyoth...@gmail.com, 
histonet@lists.utsouthwestern.edu
Date:   11/25/2014 12:21 AM
Subject:RE: [Histonet] Formalin smell in the last paraffin station 
in vip  6   tissue tek
Sent by:histonet-boun...@lists.utsouthwestern.edu



Hi
If the paraffin are overheated it gives abnormal odor.
Recheck the paraffin bath temperature and the paraffin melting point then 
make sure to adjust the bath temperature at 2 degrees above the melting 
point.
Please update me.


Best Regards,


Jamal M. Al Rowaihi  Anatomic Pathology Supervisor   | Al 
Borg Medical Laboratories |  Mobile +966 503629832| 
j.rowa...@alborglaboratories.com 
Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| 
Phone: +966 12 670 0099| Fax: +966 12 676 4984 | 
www.alborglaboratories.com

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu [
mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Arun Jyothi 
S.P
Sent: Tuesday, November 25, 2014 9:53 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Formalin smell in the last paraffin station in vip 6 
tissue tek

Dear all
After processing in vip 6 the last paraffin has a strong odour of formalin

Have anybody experienced the same
Any ideas why it is happening.

Arun
Kuwait
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[Histonet] Help with muscle

[Jennifer MacDonald]

Hello All,
I hope I am not asking a dumb question. As I know that everyone on the 
histonet is very knowledgeable I was hoping to get some suggestions 
regarding processing tissue for neuromuscular junction staining. We are a 
neuro research lab and want to quantify neuromuscular junction on treated 
and non-treated rat gastrocnemius. We need the whole muscle for 
quantifications. The first problem we encountered when attempting to do 
this on PFA perfused and post fixed whole muscle was negative staining 
with SV2 antibody (presynaptic) but nicely stained alpha bungarotoxin 
(post synaptic) end plates. After a few attempts with no success we 
decided to freeze the whole muscle in dry ice and isopentane. First off we 
are having issues with the middle of the muscle freezing completely even 
after leaving it in solution for more than 10 min. Secondly while we do 
get nice staining with SV2 and bungarotoxin in some of the endplates we 
don’t see colocalization in most of the NMJ’s. One possibility that I was 
thinking could be causing this is that when the muscle is being picked up 
on the slide (cryosections) it is not laying completely flat on the slide 
(we tend to have to focus back and forth quite a bit) so we see the 
SV2/bungarotoxin near each other but not overlapping. One other thing I 
thought might be occurring is that when the muscle is being frozen it is 
retracting causing a shift in the presynaptic/postsynaptic NMJ. What is 
the best way to process the tissue, fixation or freezing? Any suggestions 
are greatly appreciated.
Thanks,
Leslie
Leslie Garcia
Senior Histologist
Clive Svendsen Lab
Board of Governors Regenerative Medicine Institute Cedars-Sinai Medical 
Center
8700 Beverly Blvd.
AHSP 8405
Los Angeles, CA 90048
Phone - 310-248-8571
Web - http://www.cedars-sinai.edu/RMI
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Re: [Histonet] Storage of Glacial Acetic Acid

[Jennifer MacDonald]

We store ours in the acid cabinet.  It has a freezing point of 16.6 
degrees Celsius so it needs to be kept at room temperature.



From:   Laurie Colbert lcolb...@pathmdlabs.com
To: Histonet Post (histonet@lists.utsouthwestern.edu) 
histonet@lists.utsouthwestern.edu
Date:   10/16/2014 12:11 PM
Subject:[Histonet] Storage of Glacial Acetic Acid
Sent by:histonet-boun...@lists.utsouthwestern.edu



How do you store GAA?  As I recall, it should not be stored with other 
acids in an acid cabinet.  I'm think it should be stored with flammables??

Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA  90048
(323) 648-3214 direct
(424) 245-7284 main lab

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[Histonet] RDO

[Jennifer MacDonald]

Does anyone know what the O signifies in RDO?  Optimal?

Thanks,
Jennifer
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Re: [Histonet] Another TGIF Friday...

[Jennifer MacDonald]

A slightly different version:

The Gospel According to the Tech

TRIANGLE BIOMEDICAL SCIENCES
Durham, NC 27705 (919) 477-9283
A Pathologist   Leaps tall buildings in a single bound,
is more powerful than a locomotive,
is faster than a speeding bullet,
walks on water,
gives policy to God.

An InternistLeaps short buildings in a single bound,
is more powerful than a switch engine,
is just as fast as a speeding bullet,
walks on water if sea is calm,
talks with God. 

A Surgeon   Leaps short buildings with a running 
start,
  and favorable wind,
is almost as powerful as a switch engine,
walks on water in an indoor swimming pool,
talks with God if special permission is 
approved.

A General Practitioner  Barely clears a Quonset hut,
loses tug of war with a locomotive,
can fire a speeding bullet,
swims well,
is occasionally addressed by God.
 
A Gynecologist  Makes high marks on walls when trying toleap 
tall buildings,
is run over by a locomotive,
can sometimes handle a gun without 
inflicting self injury,
is all wet,
talks to animals.

A Psychiatrist  Runs into buildings,
recognizes locomotives two out of three 
times,
is not issued live ammunition,
can stay afloat with a life jacket,
talks to walls.

A Medical Student   Falls over doorstep when trying to enter 
buildings,
says “look at the choo-choo”,
wets himself with a water pistol,
plays in mud puddles,
mumbles to himself.

A Histologist   Lifts buildings and walks under them,
kicks locomotives off the tracks
catches speeding bullets in his/her teeth 
and eats them,
freezes water with a single glance,
is GOD. 



From:   sarah.dys...@stdavids.com
To: histonet@lists.utsouthwestern.edu
Date:   10/10/2014 08:51 AM
Subject:[Histonet] Another TGIF Friday...
Sent by:histonet-boun...@lists.utsouthwestern.edu



Does anyone have that thing handy that says what different positions in 
the histology lab do?  It's like Superman, leaping buildings and such...

Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP)
Pathology Supervisor
St. David's North Austin Medical Center
12221 North Mopac Expressway
Austin, Texas  78758
(512)901-1220

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Re: [Histonet] Glacial Acetic Acid subs.

[Jennifer MacDonald]

vinegar.  you can buy cleaning vinegar.  It is acetic acid, but not 
concentrated.



From:   Armstrong, Karoleigh T karoleigh.armstr...@armc.net
To: histonet@lists.utsouthwestern.edu 
histonet@lists.utsouthwestern.edu
Date:   09/30/2014 11:09 AM
Subject:[Histonet] Glacial Acetic Acid subs.
Sent by:histonet-boun...@lists.utsouthwestern.edu



Does any one know of a substitute for Glacial Acetic Acid? We use it to 
clean the lines on the VIP and in the Eosin. Our Corp. heads want to 
eliminate Glacial in concentration form from all its hospitails.
Thanks,
Karoleigh Armstrong, HT (ASCP)


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RE: [Histonet] Non-cert. histo classes

[Jennifer MacDonald]

I agree with Tim.  Any additional skills in histology will make them more 
marketable.  EM and paraffin microtomy are very similar in principle.

As of yet an AA/A is not required to take the HT (ASCP) certification 
exam.  Route 1 states graduation from a NAACLS accredited program and 
there are still some NAACLS accredited programs that offer a certificate 
and do not require an AA or AS.  The NSH appealed to NAACLS to change one 
of the Standards to require all NAACLS HT programs to either award an 
AA/AS degree or require students to have one before earning the 
certificate.  The changes to the standard went out for public comment at 
the beginning of the summer.  The NAACLS BOD meeting is September 18-19. 
The Board will vote then to either recommend or not recommend the changes 
to the standard.  If it is approved by the BOD there will be a transition 
period to allow those programs that do not offer or require an AA/AS to 
meet the standard.This does not affect those that are already certified, 
nor does it change the ASCP requirements.

Jennifer



From:   Morken, Timothy timothy.mor...@ucsfmedctr.org
To: 'Jon Krupp' jkr...@deltacollege.edu, 
histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Date:   09/02/2014 10:38 AM
Subject:RE: [Histonet] Non-cert. histo classes
Sent by:histonet-boun...@lists.utsouthwestern.edu



Jon,

It is not a bad idea. Ideally a person going into the field would have a 
good formal education in the field. However, 99.9% of the people working 
in the field did not go through a formal program, but learned on the job. 
Therefore, a person who was exposed to ANY formal education in paraffin 
histotechnology (processing, cutting, staining, special stains) would be 
well ahead- OJT is highly variable in quality as you might guess. A lot of 
what is learned in biological EM is transferable to paraffin - fixation, 
processing, even sectioning principles are the same. The difference is 
really in medium and staining chemicals, and of course, the microscope 
used. 

There are obviously a lot more jobs in histology than in EM. Biotech does 
not necessarily require certification and it is not needed as a regulatory 
requirement of their work (and a combined EM/histotech is more valuable; 
throw in some DNA/RNA work (ISH, FISH, PCR and you have a supertech!). 
Hospitals and other medical labs usually do not require certification 
(like or not!) for entry level (or even higher levels in many cases) but 
if they want you to have it they will usually have a time period that they 
require you to get it - certification eligible or certification within 
one year or something like that. Many of our histotechs came from the 
UCSF research labs where they learned a bit of paraffin sectioning and 
then applied in our lab. All have done well and all have gone on to get 
certified.

Acquiring certification requires working one year under a board certified 
pathologist, and taking a test. It takes some study, but that is the route 
most people take.

The most important part is that certification now requires an AA degree at 
the minimum with certain levels of biology and chemistry courses. Those at 
Delta would meet that standard pretty easily if they are in the EM program 
anyway. 

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special 
Studies
UC San Francisco Medical Center
San Francisco, CA


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu [
mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jon Krupp
Sent: Tuesday, September 02, 2014 9:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Non-cert. histo classes

Greetings

I am a regular reader of this list, but from a peripheral setting.

I work in an electron microscopy certificate program. Our students are 
prepared to do either or both certificates in EM, biological and 
materials.

During their biological training, students are instructed in thin 
sectioning, specimen prep., etc. I would like to get ideas about how 
valuable adding more light microscopy specimen prep, staining, and 
sectioning might be for these students. Would it be good for them to have 
these skills and knowledge but not a histotech cert.?

I thought about doing a full cert. program, but am daunted by the 
requirements to set one up and I don't want to compete with other better 
established programs near by.

Bottom line is I would like to know if teaching basic histo tech skills, 
w/o certification, is a viable path. Could students leverage these skills 
into jobs at non-health care type facilities? Could having these skill 
help them complete a certificated program if they wanted to take that 
direction later? 

Your input will be valuable to both me and my students.

Thanks

Jon

Jonathan Krupp
Applied Science, Business  Technology
San Joaquin Delta College
5151 Pacific Ave.
Stockton, CA  95207
209-954-5284
jkr...@deltacollege.edu

Find us on Facebook @

Re: [Histonet] Weight Loss/Weight Gain Decal

[Jennifer MacDonald]

I believe this was originally from Patsy Ruegg

Decalcification End Point: Weight Loss, Weight Gain


1.  Blot sample to remove excess fixative
2.  Weigh bone in mg, record as beginning weight
3.  Next day, rinse bone, blot and weigh bone daily, record weight. 
Change decalcifying solution to refresh acid OR EDTA. Return bone to fresh 
decalcifying solution.
4.  When bone begins to GAIN weight, remove from decalcifying 
solution, rinse and process.

YOU MUST WEIGH IN MILLIGRAMS FOR ACCURACY

Once calcium is totally removed (bone loses weight as this happens), water 
replaces the calcium and weight begins to go up.  This is the point at 
which calcium should be totally gone.  The original method used a chemical 
test at the end to insure no calcium was in the decalcifying solution.  If 
you do this, you cannot stir the solution during decalcification.  Be sure 
to suspend bone in the solution to insure all sides of bone are in contact 
with decalcification solution. 



From:   Wait, Trevor Jordan wa...@livemail.uthscsa.edu
To: histonet@lists.utsouthwestern.edu 
histonet@lists.utsouthwestern.edu
Date:   08/11/2014 12:29 PM
Subject:[Histonet] Weight Loss/Weight Gain Decal
Sent by:histonet-boun...@lists.utsouthwestern.edu



Hello all! I'm currently doing some decalcification and was curious if 
anyone had some particular advice about the weight loss/weight gain 
method. I understand that when the decalcification process is complete, 
the tissue block will begin to increase in weight. However, I'm confused 
when I should record the weight for the block once they have been taken 
out of the EDTA solution. You see, for the times I weighed the blocks 
before... the weights were a little skewed because there were differing 
amounts of solution on the blocks while they were sitting on the balance. 
I just want to standardize my protocol a little more so that I can be sure 
the block is actually gaining weight due to the calcium loss rather than 
just extra solution sitting on the outside of the block. Would letting the 
blocks sit out of solution for about 30 minutes before being weighed help 
with the matter? I know that the blocks take on water once they are 
completely decalcified so I'm not sure how much this will affect that.


Trevor Jordan Wait
University of Texas Health Science Center, San Antonio
Class of 2017 MD Candidate
Abilene Christian University Class of 2013 Graduate
B.S.  Biochemistry
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Re: [Histonet] On the lighter side...

[Jennifer MacDonald]

31 years

Jennifer MacDonald



From:   Douglas Porter doug.por...@caplab.org
To: histonet@lists.utsouthwestern.edu
Date:   08/07/2014 11:36 AM
Subject:[Histonet] On the lighter side...
Sent by:histonet-boun...@lists.utsouthwestern.edu



How long have you been a registered histotech?  36 years here.  You???

 

Douglas A. Porter, HT (ASCP) 
Grossing Technician 
IT Coordinator

Cancer Registrar 


CAP-Lab, PLC 
2508 South Cedar Street 
Lansing, MI 48910-3138 

517-372-5520 (phone) 
517-372-5540 (fax) 

 mailto:doug.por...@caplab.org doug.por...@caplab.org 

 http://www.caplab.org/ www.caplab.org 

 

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Re: [Histonet] Xylene Substitute Protocols

[Jennifer MacDonald]

We have found that Permount works best with the aliphatic hydrocarbons.

Sent from my iPad

 On Jul 24, 2014, at 10:01 AM, Hilliard, Dawud Deshawn
dawud_hilli...@med.unc.edu wrote:

 Hello all,

 Would anyone mind sharing their xylene substitute protocols for their
automatic staining, to include the mountant type for coverslipping? We have
tried a few, but without much success. We are currently trying Leica's
Sub-X, also without much success.

 I greatly appreciate the insight!

 Dawud Hilliard
 MPM, HTL (ASCP) QIHC
 Facility Director
 Animal Histopathology Core Lab
 Lineberger Comprehensive Cancer Center
 University of North Carolina - Chapel Hill
 Chapel Hill, North Carolina 27599
 (919) 966-3653

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Re: [Histonet] HS IHC project

[Jennifer MacDonald]

T cell and B cell markers work well with human spleen.



From:   Patsy Ruegg pru...@ihctech.net
To: Histonet@Lists. Edu histonet@lists.utsouthwestern.edu
Date:   07/21/2014 11:08 AM
Subject:[Histonet] HS IHC project
Sent by:histonet-boun...@lists.utsouthwestern.edu



Friends,

I am going to help a local HS class (a Biotech class) do
 a simple IHC project.  This class already processes tissue, embeds, 
sections and does an HE stain.  My husband fixed up an old black AO
 microtome for them to use and they actually cut sections.  They cannot 
use human tissue there so what they have available is mouse spleen 
already ffpe.  I was thinking of using a rab antibody that works on ffpe
 ms spleen.  Ki67 comes to mind?  It has been a while since I was in the
 lab so I am consulting with you all to make sure a rab poly or rab mono
 Ki67 will indeed stain a ffpe ms spleen?  If you have evidence of this 
can you tell me which ab was used successfully.  If you can think of a 
better rab ab to use on the ffpe ms spleen for this project let me 
know.  It would help if we could keep it simple for antigen retrieval.

One of our kind vendors will be donating IHC reagents for this HS class 
project.

I hope the IHC meeting is going well in Vegas, wish I was there.

Cheers,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
pru...@ihctech.net
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[Histonet] California Society for Histotechnology

[Jennifer MacDonald]

Don't miss out on some great work shops.  The deadline for registration 
has been extended to this Friday and if you still need a room that can be 
arranged. 

For registration:
http://californiahistology.org/events.html

For rooms:
contact Hilton reservations coordinator Yvette Moradians at 
818-596-4506
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Re: [Histonet] Semen analysis training

[Jennifer MacDonald]

I would contact any clinical lab in your area.  When I worked in the 
clinical lab we did semen analysis in Hematology. 
Jennifer



From:   Chris Duffett cduff...@pathlinelabs.com
To: 
Cc: histonet@lists.utsouthwestern.edu 
histonet@lists.utsouthwestern.edu
Date:   04/15/2014 10:18 AM
Subject:[Histonet] Semen analysis training
Sent by:histonet-boun...@lists.utsouthwestern.edu



Looking for a location in the New York City or northern New Jersey area 
that might be able to help with training 1or2 techs to so semen analysis 
for motility and morphology.
We know of the Cleveland clinic program but sea a bit too far to send 
staff.
Any insight will be helpful

Sent from my iPhone
Chris Duffett
Manager, Laboratory Development
Pathline/Emerge
845-709-4246


 On Apr 15, 2014, at 1:11 PM, Klaus Dern klaus.der...@gmail.com 
wrote:

 In reference to Ms. Weems, Joyce K. posting of Dec.17.2013 ( Microtome
 Service ). I would like to add that this information is specifically 
about
 retrofitting the advance mechanism on the following Microtomes which are
 not supported by the manufacturer anymore with an adjustment feature to
 elliminate excessive spindle play. ( thick  thin sections ).

 The Microtomes in Question are.

 Reichert/Jung: 2030
 Leica: RM 2125
 Leica: 2030 Biocut
 Leica/Jung: 2035
 Leica: CM-1850 Cryostat
 Sakura: SRM 200

 For Information, please contact.

 Klaus Dern

 Phone: 706  635-8840
 Fax: 706  635-3074
 e.mail:  klaus.der...@gmail.com
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Re: [Histonet] white Sakura ice trays

[Jennifer MacDonald]

We purchased ours directly from Sakura.

Sent from my iPad

 On Apr 11, 2014, at 1:04 PM, Delray Beach Pathology Kari Simeone
ksime...@leavittmgt.com wrote:

 Can anyone tell me what vendor I can purchase the white Sakura ice trays
for blocks that you can freeze (liquid inside) and put blocks on when
cutting. Hope this run on sentence makes sense! Thanks in advance. :-)



 Kari M Simeone

 Histology/Immunohistochemistry Specialist Supervisor

 Alternate Laboratory Supervisor

 Delray Beach Technical Laboratory

 ksime...@leavittmgt.commailto:ksime...@leavittmgt.com





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disclosure under applicable law. If you have received this message in
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information. Please contact the sender immediately by return e-mail and
delete the original message.


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[Histonet] California Society for Histotechnology Symposium

[Jennifer MacDonald]

The 38th Annual Symposium/Convention for the California Society will be 
held May 2-4, 2014 at the Hilton Woodland Hills in southern California.
Lots of great workshops and vendors!

For more information and to download the program visit:  
http://californiahistology.org/events.html
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Re: [Histonet] Best Study material for HTL

[Jennifer MacDonald]

There is a newer addition of this student guide available through the NSH 
or ASCP.  If you are a member there is a reduced price.

http://www.ascp.org/Store/Books/BOC-Study-Guide-Histotechnology.html#StoreList




From:   Tony Auge tony.a...@gmail.com
To: joelle weaver joellewea...@hotmail.com
Cc: histonet@lists.utsouthwestern.edu 
histonet@lists.utsouthwestern.edu
Date:   03/03/2014 08:54 AM
Subject:Re: [Histonet] Best Study material for HTL
Sent by:histonet-boun...@lists.utsouthwestern.edu



This study guide is pretty handy:

http://www.amazon.com/Practice-Questions-Histotechnology-Examinations-Registry/dp/089189473X/ref=sr_1_1?ie=UTF8qid=1393865047sr=8-1keywords=histotechnology+bor


I used that and Carson to study for my HTL.


-- 

Tony Auge HTL (ASCP) QIHC
Histology Supervisor - Chandler Pathology Services
Cell: (651) 373-4768
Email: tony.a...@gmail.com
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[Histonet] aqueous mounting medium

[Jennifer MacDonald]

Does anyone have a good recipe for aqueous mounting medium?
Thanks,
Jennifer
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Re: [Histonet] aqueous mounting medium

[Jennifer MacDonald]

Enzyme histochemistry

 On Feb 19, 2014, at 6:42 PM, Lee  Peggy Wenk lpw...@sbcglobal.net
wrote:

 to be used for what?
 - Lipids - ORO, Sudan black B?
 - Immunofluorescence - which dye?

 Different needs, different requirements.

 Peggy A. Wenk, HTL(ASCP)SLS

 -Original Message-
 From: Jennifer MacDonald
 Sent: Wednesday, February 19, 2014 12:27 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] aqueous mounting medium

 Does anyone have a good recipe for aqueous mounting medium?
 Thanks,
 Jennifer
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Re: [Histonet] Dried out liver bx's

[Jennifer MacDonald]

We had the same problem with GI biopsies. The problem was always with the
same tech. She was blasting the blocks with freezing spray and they were
freezer burnt.

 On Feb 19, 2014, at 11:49 AM, SARAH GIBSON sara...@hotmail.com wrote:

 We are having problems with some of our small liver bx looking burned and
dry. Does anyone have any suggestions on ways to fix this problem and any
techniques while sectionly to get a better quality section. Thanks for any
help.

 Sent from my iPad
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Re: [Histonet] Warthin Starry Help

[Jennifer MacDonald]

Hydroquinone has a short shelf life once opened.

 On Jan 14, 2014, at 6:59 AM, Campbell, Tasha M. tmcampb...@fmh.org
wrote:

 I need help with warthin starry stain please.  I am a very small lab
 trying to save money so I want to make the reagents myself.  I have
 sigma Aldrich dry reagents and have been making the reagents and
 following the instructions correctly and I did get the stain to work
 wonderfully when I first started.  Then suddenly it quit working and I
 cannot get it to work again.  I have made fresh reagents day after day
 and I have a pH of 3.8 for the acidulated water.  The tissue will stain
 normally with the yellow to brown background and I the developing
 solution turns black when it is left on paper but the h.pylori organisms
 are not staining.  I am getting very frustrated and need some help
 please.



 Thank you.



 Tasha Campbell, HTL

 tmcampb...@fmh.org



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Re: [Histonet] Best place to Buy Slides boxes preferably True North.

[Jennifer MacDonald]

Contact Russ at StatLab. We bought ours from StatLab. Good quality latches 
compared to some we bought. 

Russell Komae
StatLab Medical Products
Southern California Account Manager
407 Interchange Street | McKinney, TX 75071
t: 310.529.4465 | f: 972.436.1369
rko...@statlab.com | www.statlab.com


 On Dec 11, 2013, at 1:52 PM, Lewis, Patrick 
 patrick.le...@seattlechildrens.org wrote:
 
 Hi Everyone,
 
 Can anyone tell me the cheapest place to get True North Slide boxes (100 
 slides purple box)
 
 VWR has them for about $33.00 EACH.
 
 We get a shipping discount with VWR but there has got to be a cheaper 
 supplier.
 
 Baring that, can anyone recommend a good 100 slide storage box.   Preferably 
 in the $10-20.00 per box range.
 
 
 
 
 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is 
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Re: [Histonet] freezing spray

[Jennifer MacDonald]

Reasons against the use of freezing spray for frozen sections:
From the Federal Registry 1910.1030:  “All procedures involving blood or 
other potential infectious materials shall be performed in such a manner 
as to minimize splashing, spraying, spattering, or generation of droplets 
of these substances.”
From NCCLS (now CLSI) Document M29-T:  “Frozen sections done on unfixed 
tissue pose a high risk because accidents are common.  Freezing of tissue 
does not inactivate infectious agents.  Freezing propellants under 
pressure should not be used for frozen sections as they may cause 
spattering of droplets of infectious material.”




From:   Horn, Hazel V hor...@archildrens.org
To: histonet (histonet@lists.utsouthwestern.edu) 
histonet@lists.utsouthwestern.edu
Date:   10/24/2013 11:47 AM
Subject:[Histonet] freezing spray
Sent by:histonet-boun...@lists.utsouthwestern.edu



Our hospital has not allowed freezing spray to be used in the frozen 
section lab for many years.  We now have a new group of doctors who want 
to use the spray.  The docs think the frozen sections take too long to 
freeze.  Yet, they meet the frozen section TAT for more than 98% of our 
cases.  I think it's worth not using it for the one case where no one 
suspects TB but the patient will be positive.

Do you allow freezing spray in your frozen section lab?


Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.orgmailto:hor...@archildrens.org
archildrens.orghttp://www.archildrens.org/







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The information contained in this message may be privileged and 
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notify 
us immediately by replying to the message and deleting it from your 
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Re: [Histonet] Microtome Blade safety, in or out when not in use?

[Jennifer MacDonald]

We save the blade to use for trimming.  We store the blade in a plastic 
5-slide mailer.



From:   Leah Simmons leah_simmon...@hotmail.com
To: histonet@lists.utsouthwestern.edu 
histonet@lists.utsouthwestern.edu
Date:   10/24/2013 06:36 PM
Subject:[Histonet] Microtome Blade safety, in or out when not in 
use?
Sent by:histonet-boun...@lists.utsouthwestern.edu



Hello all :-)
I am doing a quick microtome blade safety survey,
When you finish work, do you leave your blade in the microtome behind the 
blade guard or do you take it out?
If you take it out and it is a new blade or a blade still useful for 
trimming  where do you store it?
Thank you for your feedback, I really appreciate it.
Regards
Leah Simmons
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[Histonet] California Society for Histotechnology Meeting

[Jennifer MacDonald]

The California Society for Histotechnology annual symposium is scheduled 
for May 2-4, 2014 at the Hilton Woodland Hills (southern CA, LA area).  We 
are now accepting abstracts.  If you are interested in speaking please 
contact me and I will send you an application.
Thank you,
Jennifer MacDonald
CSH Secretary

jmacdonald...@gmail.com
jmacdon...@mtsac.edu
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Re: [Histonet] HT HistoDeck question...

[Jennifer MacDonald]

We also use this oil red O method and use the 40% formaldehyde.
The questions lacks enough information to correctly answer it.  I am sure 
the author of the question had something in mind and other options didn't 
occur to him/her at the time.
Jennifer




From:   Grantham, Andrea L - (algranth) algra...@email.arizona.edu
To: 
Cc: HISTONET histonet@lists.utsouthwestern.edu
Date:   10/03/2013 08:33 AM
Subject:Re: [Histonet] HT HistoDeck question...
Sent by:histonet-boun...@lists.utsouthwestern.edu



I'd go with A, but it really depends on what you are going to do with 
the sections after fixation.

In the protocol for Oil Red O in Freida's second edition (that I use 
almost daily combined with some steps from PolyScientific's ORO protocol), 
step #1 says to fix in 40% formaldehyde. Doesn't say vapors so I plunk the 
slides in the liquid 40%.
I have fixed frozen sections for regular HE and other stains as well in 
40% formaldehyde and they come out beautiful.
Unless there is some specific request or reason to use cold acetone or 
alcohol this is what I use.

Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algra...@email.arizona.edumailto:algra...@email.arizona.edu
Tel: 520.626.4415 Fax: 520.626.2097





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RE: [Histonet] HT HistoDeck question...

[Jennifer MacDonald]

the protocol calls for 1 minute in the 40% formaldehyde and then rinse the 
sections well.



From:   Rathborne, Toni trathbo...@somerset-healthcare.com
To: 'Jennifer MacDonald' jmacdon...@mtsac.edu, Grantham, Andrea L 
-   (algranth) algra...@email.arizona.edu
Cc: HISTONET histonet@lists.utsouthwestern.edu, 
histonet-boun...@lists.utsouthwestern.edu 
histonet-boun...@lists.utsouthwestern.edu
Date:   10/03/2013 11:42 AM
Subject:RE: [Histonet] HT HistoDeck question...
Sent by:histonet-boun...@lists.utsouthwestern.edu



For those of you who use the 40% formaldehyde, how long is you fixation 
time on frozen slides? We use 10% NBF with 1 minute to fix, but sometimes 
it gets hectic if you have multiple frozens all at once. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu [
mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer 
MacDonald
Sent: Thursday, October 03, 2013 2:04 PM
To: Grantham, Andrea L - (algranth)
Cc: HISTONET; histonet-boun...@lists.utsouthwestern.edu
Subject: Re: [Histonet] HT HistoDeck question...

We also use this oil red O method and use the 40% formaldehyde.
The questions lacks enough information to correctly answer it.  I am sure 
the author of the question had something in mind and other options didn't 
occur to him/her at the time.
Jennifer




From:   Grantham, Andrea L - (algranth) algra...@email.arizona.edu
To: 
Cc: HISTONET histonet@lists.utsouthwestern.edu
Date:   10/03/2013 08:33 AM
Subject:Re: [Histonet] HT HistoDeck question...
Sent by:histonet-boun...@lists.utsouthwestern.edu



I'd go with A, but it really depends on what you are going to do with 
the sections after fixation.

In the protocol for Oil Red O in Freida's second edition (that I use 
almost daily combined with some steps from PolyScientific's ORO protocol), 
step #1 says to fix in 40% formaldehyde. Doesn't say vapors so I plunk the 
slides in the liquid 40%.
I have fixed frozen sections for regular HE and other stains as well in 
40% formaldehyde and they come out beautiful.
Unless there is some specific request or reason to use cold acetone or 
alcohol this is what I use.

Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algra...@email.arizona.edumailto:algra...@email.arizona.edu
Tel: 520.626.4415 Fax: 520.626.2097





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Re: [Histonet] Gram stain

[Jennifer MacDonald]

We have had great success with the Twort gram stain.



From:   Mesru T turke...@gmail.com
To: histonet@lists.utsouthwestern.edu
Date:   10/02/2013 10:13 AM
Subject:[Histonet] Gram stain
Sent by:histonet-boun...@lists.utsouthwestern.edu



Hi All,



Does anybody have a protocol for Gram stain on FFPE mouse intestine
sections. We are looking to distinguish between Klebsiella and Vancomycin
Resistant Enterococcus (VRE). I would like to avoid using Picric acid as
some protocols suggest.

Thanks in advance.
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RE: [Histonet] Unregistered HT

[Jennifer MacDonald]

As long as we do not need certification, licensure and minium education 
requirements we will not be recognized as Laboratory Professionals.



From:   Marcum, Pamela A pamar...@uams.edu
To: 'joelle weaver' joellewea...@hotmail.com, 'Emily Sours' 
talulahg...@gmail.com, histonet@lists.utsouthwestern.edu 
histonet@lists.utsouthwestern.edu
Date:   09/10/2013 01:12 PM
Subject:RE: [Histonet] Unregistered HT
Sent by:histonet-boun...@lists.utsouthwestern.edu



I agree we have huge gray areas and not all histology schools are as good 
as they could be for what we are facing in Histology.  I keep harping on 
the fact that until we are recognized as Laboratory Professionals we will 
stay in this limbo.  The rules determining complex testing should be 
revisited to what is done in Histology Laboratories today and not what we 
did 30 or more years ago.  The Clinical Laboratory is now so automated it 
is hard to find anyone in most areas who can even remember doing any 
manual testing.  The Micro lab is the closest to being as manual as areas 
of Histology.

I am in a small market and finding a registered Histologist is harder for 
us.  I would love to have 8 to choose from and interview.

Pam Marcum

From: joelle weaver [mailto:joellewea...@hotmail.com]
Sent: Tuesday, September 10, 2013 2:59 PM
To: Marcum, Pamela A; 'Emily Sours'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Unregistered HT

 Well I am  mostly clinical...but I think that organizations can set 
standards outside and beyond what CAP,CLIA etc stipulate. For the position 
I have now, I had to submit all my transcripts from high school up through 
masters in addition to  proof of my ASCP certification, IHC qualification, 
continuing education, and professional association activity. There is a 
lot of gray area out there. They seem to have not had trouble getting 
applicants though ( and I know this varies by market), there were over 8 
candidates for an HT opening, which I thought was a pretty good turn out.


Joelle Weaver MAOM, HTL (ASCP) QIHC

 From: pamar...@uams.edu
 To: talulahg...@gmail.com; joellewea...@hotmail.com
 CC: histonet@lists.utsouthwestern.edu
 Subject: RE: [Histonet] Unregistered HT
 Date: Tue, 10 Sep 2013 13:53:26 +

 Research is a different area and not controlled by CAP, CLIA and other 
hospital licensing or accreditation organizations. We are bound by the 
rules of these organizations and while I agree with you to a point. We do 
need minimums for training and registration by recognized licensing bodies 
when patient tissue is being processed for histological examination. I am 
sure no one thinks of this often however; there are medical legal issues 
with insurance we have that do not apply for research. It is also clear 
that registration does not mean we don't have registered people who are 
not as good as they should be for excellent patient care.

 I have worked in research and while I would not ever say the hiring of 
non-registered people is a problem for research. It is often a specialty 
area that requires knowing more than routine Histology. I have done 
plastics in research that could not ever be used in routine Histology due 
to the time factors and in some cases limited use with staining 
applications, especially IHC for some procedures. Many other areas in 
research require more specialized training than would be used in a routine 
area. I would also add some really great techs are in many phases of 
research. I know MT who work in Histology and are not registered as the MT 
BS, overrides the HT requirement for many institutions.

 Many factors must be considered for both research and routine Histology 
that cover far more than just hiring registered people in certain areas of 
the laboratory.

 Pam Marcum


 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
mailto:histonet-boun...@lists.utsouthwestern.edu [
mailto:histonet-boun...@lists.utsouthwestern.edu]mailto:[
mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily 
Sours
 Sent: Tuesday, September 10, 2013 8:20 AM
 To: joelle weaver
 Cc: histonet@lists.utsouthwestern.edu
mailto:histonet@lists.utsouthwestern.edu
 Subject: Re: [Histonet] Unregistered HT

 Do employers consider lab techs to be proficient enough? I've been doing 
ISH and immuno for 13 years, but I'm not certified as I do research. Maybe 
there aren't a lot of lab techs out there? Just wondering since you might 
be missing someone awesome by hiring only certified people.

 By bitching and bitching and bitching, they could exhaust the drama of 
their own horror stories. Grow bored. Only then could they accept a new 
story for their lives. Move forward.

 -Chuck Palahniuk, Haunted


 On Mon, Sep 9, 2013 at 6:49 PM, joelle weaver joellewea...@hotmail.com
mailto:joellewea...@hotmail.comwrote:

  All I have is a histology assistant description I put together. It
  is mostly clerical, instrument up keep and other duties. My employer

Re: [Histonet] (no subject)

[Jennifer MacDonald]

Recommended melting point of paraffin is 2-4 degrees above the melting 
point of the paraffin.  Because we really don't see paraffins that would 
have a melting point of 46, the BEST answer would be 58-70.  Perhaps not 
what we do, but the best answer for the choices provided.



From:   Martin, Erin erin.mar...@ucsf.edu
To: histonet histonet@lists.utsouthwestern.edu
Cc: Naujokas, Agne agne.naujo...@ucsfmedctr.org, Meier, Melissa 
melissa.me...@ucsfmedctr.org
Date:   09/09/2013 06:30 AM
Subject:[Histonet] (no subject)
Sent by:histonet-boun...@lists.utsouthwestern.edu



Good morning all!

One of our fellows emailed me a question that she came across while 
studying for her boards:



I'm studying for my board exam and came across questions re: paraffin 
embedding.
It reads: best temperature for paraffin embedding is
38-48
48-58
58-70.
I am getting some info on Internet that says 58 but is the range lower or 
higher than that? What do we use?

This seems to me to be an odd question because it depends on the melting 
point of the paraffin in use.  Ours melts at 58C and we embed at 60C, but 
we have also used paraffin that melts at 56C and we embedded at 58C.  Or 
am I missing something?  Does anyone have a clear cut answer to this?



Thanks everyone!

Erin

Erin Martin, Histology Supervisor

UCSF  Dermatopathology Service
415-353-7248

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Re: [Histonet] Oil red O

[Jennifer MacDonald]

I believe the question is what is the O signify.
as in OG6 the O is for orange.



From:   Rene J Buesa rjbu...@yahoo.com
To: P.E. Visser p...@xs4all.nl, histonet@lists.utsouthwestern.edu 
histonet@lists.utsouthwestern.edu
Date:   09/09/2013 12:46 PM
Subject:Re: [Histonet] Oil red O
Sent by:histonet-boun...@lists.utsouthwestern.edu



Do you mean that you were asked to do Oil Red O (ORO) stain?
It is described in any technology book.
I piece of advise: stain the nuclei with hametoxyline first and after that 
stain with ORO
René J.



From: P.E. Visser p...@xs4all.nl
To: histonet@lists.utsouthwestern.edu 
Sent: Monday, September 9, 2013 3:40 PM
Subject: [Histonet] Oil red O


Hi all

I was requested where the O stands for. who has any suggestion.



Regards Piet Visser 

Histotech Bronovo The Netherlands





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[Histonet] Help for Northern CA HT program

[Jennifer MacDonald]

Merritt College has started a HT program and is in need of clinical sites 
for their students to get hands-on experience.  If you interested in 
being a mentor for the HT students please contact Gisele Giorgi at 
profgio...@peralta.edu
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Re: [Histonet] CLIA Compliance Regulations for histology staff coverage

[Jennifer MacDonald]

California does not have licensure for histotechs.  To my knowledge 
California does not have any regulations concerning who can cut biopsies. 
There are many labs that have uncertified people working without the 
supervision of certified people.  That being said, is this the only reason 
the Pathologist wants the cases sent our during her absence?  He is the 
one that has to sign his name to the cases.



From:   Akemi Allison akemiat3...@yahoo.com
To: Histonet Histonet@lists.utsouthwestern.edu
Date:   07/18/2013 08:34 AM
Subject:[Histonet] CLIA Compliance Regulations for histology staff 
coverage
Sent by:histonet-boun...@lists.utsouthwestern.edu



Good morning to all in Histoland!
 
Hopefully, one of you can help me with a question which was proposed to me 
by a fellow histologist working in a very small GI histology lab. 
 
Here is what information she gave me:  She is training a histology 
assistant in house to do all the histology technical responsibilities at 
the request of the GI doctors.  She said her OJT assistant is doing a 
great job technically, but she has not yet registered in school to finish 
her  AA Degree so she can sit for the HT exam.  She has been working under 
her instruction for 1 year.  Her lab is California State and CLIA 
licensed.
 
The GI doctors want the assistant to cover for her while she is on 
vacation.  The pathologist who is the medical director does  not think 
that CLIA Regulations allows this and wants the specimens sent out during 
her absence.  She needs the CLIA Regulations stating what the guidelines 
are so her lab is complying to regulations.  She didn’t want to call CLIA 
because it would send a RED FLAG up.  I also didn’t want to call them 
because they may ask me what the name of the lab was that was proposing 
this question.  
 
I told her that this was most likely illegal, but she needs it in black 
and white.  Do any of you have that information or a web link to go to at 
your finger tips? She needs the regulations by next week.
 
Thank you in advance for your help,
Akemi
 
Akemi Allison-Tacha, BS, HT/HTL (ASCP) 
Pathology Manager
Monterey Bay GI Consultants
23 Upper Ragsdale Drive, Suite 200
Monterey, CA 93940
(381) 375-3577  X117
Email: aalli...@montereygi.com
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[Histonet] Positions in SF area

[Jennifer MacDonald]

I have a graduate that is looking for a Histotechnician position in the 
Bay area.  He is currently working for a dermatology lab.  He would like 
to relocate to the Bay area.  If you know of any positions would  you 
please let me know.  Thank you,
Jennifer MacDonald
Mt. San Antonio College
909-274-4884
jmacdon...@mtsac.edu
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[Histonet] immuno moisture chambers

[Jennifer MacDonald]

Ted Pella has some options.  We use the Immunostain Moisture Chambers 
#21049, but there are others.

http://www.tedpella.com/glasswar_html/slidedsh.htm#21049
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[Histonet] Wage and Vacancy Survey

[Jennifer MacDonald]

I was able to locate the vacancy survey that the ASCP published, but 
cannot find the wage survey.  Does anyone have information?  It was 
supposed to be published in the November 2012 issue of LabMedicine.
Thanks,
Jennifer MacDonald
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[Histonet] Need Current Histology Benchmark Data

[Jennifer MacDonald]

Does anyone have current benchmark data? – like expected average # 
blocks/slides, per day ? 

 
 

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Re: [Histonet] Gram stain

[Jennifer MacDonald]

We use a Twort stain with my students.  They have been doing the gram 
stain the last couple of days and getting great results.  I will look for 
the entire procedure.



From:   Fawaz Zouabi fawaz.zou...@sswahs.nsw.gov.au
To: histonet@lists.utsouthwestern.edu
Date:   05/23/2013 09:32 PM
Subject:[Histonet] Gram stain
Sent by:histonet-boun...@lists.utsouthwestern.edu



Hi histonetters
 does any one have a good working method for GRAM stain ???
I used mod brown's variation and my Neg bact still does not stain red. 
 
Fawaz Zouabi
Histo-Technologist 
 Department of Forensic Medicine Glebe NSW Forensic  Analytical Science 
Service - FASS
P O Box 90 Glebe NSW 2037 | Tel +612 8584 7842 | Fax +612 95664573 


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