RE: [Histonet] De-differentiated tumor vs. Radiation effect
Used to report cervical cytology post radiation; many of the cells were extremely bizarre and to my mind looked malignant. Fraid I coped out and reported them as 'abnormal cells present in a post radiation smear, malignancy cannot be excluded'. These changes lasted for many years until they settled down to give a normal looking 'post menopausal smear'. Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lott, Robert Sent: 22 September 2009 22:58 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] De-differentiated tumor vs. Radiation effect Hi Everyone, One of our pathologist has some IHC results that is a bit confusing... Patient had previous Squamous cell Ca of the lung... had long courses of radiation and Chemo...eventually died... At autopsy, the tumor looks much more primitive and bizarre than before... radiation effect, etc... Are the any published papers that have looked at the effects of radiation on pre and post IHC staining Any help would be appreciated. Robert Robert L. Lott, HTL(ASCP) / Manager, Anatomic Pathology Trinity Medical Center/ LabFirst/ 800 Montclair Road / Birmingham, AL 35213 / 205-592-5388 -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Separation Artifact
If its happening to an entire batch but not to other batches then you can sorta rule out fixation unless you use different batches of fixative for different batches of samples; logical yes? To put it another way if you use the same fixative and fixation time on all batches but only one is affected then it must be an issue with the machine. Not knowing which one you are using makes this difficult but sometimes they go wrong or there's the wrong fluid dispensed. If however you are using different batches of fixatives on different batches of samples you may have one batch of fixative that's incorrectly made up. Hope that helps. Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of theci...@yahoo.com Sent: 21 September 2009 21:27 To: Histonet Subject: [Histonet] Separation Artifact Hello histonet! I'm trying to figure out the cause of some artifact in my ffpe he skin slides. Every once and a while I get a batch of slides with this strange separation artifact mostly around melanocytes. Its like there is a space around each cell(s). Also the collagen seems to have some odd stretching. I tried recuting to make sure there was no microtomy issues but the artifact remains. I check my waterbath temp / soaked the blocks. Not soaked the blocks. I'm at a loss. This happens to entire processing batch. The doctors say they are still pathologically readable but I still want to find a way to fix this. Sent from my Verizon Wireless BlackBerry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] air quality in the lab
That's how often you have to turnover the water in a Koi Pond; 4 times and hour. Do you keep fish? Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 16 September 2009 16:53 To: godsgal...@aol.com; histonet@lists.utsouthwestern.edu; Merced M Leiker Subject: Re: [Histonet] air quality in the lab From the recesses of my sometimes foggy memory, the figure of 4 exchanges/hour has popped up. Perhaps somebody else has some other figure. René J. --- On Wed, 9/16/09, Merced M Leiker lei...@buffalo.edu wrote: From: Merced M Leiker lei...@buffalo.edu Subject: Re: [Histonet] air quality in the lab To: godsgal...@aol.com, histonet@lists.utsouthwestern.edu Date: Wednesday, September 16, 2009, 11:11 AM that's a good one. I'd like to know that one, too. --On Wednesday, September 16, 2009 10:40 AM -0400 godsgal...@aol.com wrote: Does anyone know what the regulations or requirements are for the air turnover in the lab? Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] type of paraffin and polymer
Ester Wax Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: 11 September 2009 17:10 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] type of paraffin and polymer Dear Histonetters, I quite need answer as soon as it possible, PLEASE! I am working with mouse tissue and tumors. What type of paraffin is the best for processing and embedding to cut 4µ nice sections? Thanks in advance, Naira ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] FNA SLIDES
Adequacy is carried out by the Biomedical Scientist usually in the UK. I make a couple of air dried per passage and stain Diff Quick; you can then give an indication of adequacy within 5 min or so. Usually if the FNA is performed properly you don't get much material;; for example breast and LN's are usually sparse and you get maybe a couple of slides (we don't check these for adequacy as the Site is easily re-needled). Pancreas, Thyroid and Lung are another matter. Usually CT orientated FNAC of the lung tend not to be too cellular but sometimes they are; I used to stain a couple for adequacy and make as many slides as possible. The problem is if you make loads of slides and malignant cells are not apparent in those you stain then you have to look at all the others; tough. If there are malignant cells abundant then you ought to look at all the slides just in case they hold diagnostic information. In the end we did 6 slides and washed the remainder into a pot with saline and did Cytospins if appropriate. Pancreatic FNACs taken under a ultra sound flexible scope can also be very cellular (blood) and the above statement holds. Thyroid's can be very bloody, very bloody; trick is not to pull on the plunger but let it seep into the syringe. Too much blood and what little you have gets well diluted!! Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jane C. Moose Sent: 31 August 2009 19:34 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FNA SLIDES A question has arisen for us- How many slides do you (should you) make per pass for pathologist for adequacy and/or diagnosis? What about CT guided biopsies of liver, lung, masses etc. Thanks in advance for your input. Jane Jane Moose LIS Coordinator Newberry County Memorial Hospital Newberry, SC 29108 P-803-405-7129 F- 803-405-7474 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Rat's lung
Immerse it in water fully inflated and see how much water is displaced; is that Avogadro hypothesis or some other Greek dude? Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of tahs...@brain.net.pk Sent: 23 July 2009 16:51 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Rat's lung Dear All, How the volume of Rat's lung can be meausured? Thanks advance Muhammad Tahseen Histology Supervisor SKMCHRC lAHORE,pAKISTAN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] histology for kids
Depends on the age of the kids as I don't understand the term 'grade-school'. What I did for kids around 10 yesrs old or so was to go to the Butchers and get some Ox kidney, heart and liver. I prepared slides from them, took a microscope to let them see the structure and also took scapels for them to cut up the animal tissue. Odd how many kids haven't handled animal organs or raw meat. Anyways be careful of the scapel maybe you risk adverse Americans ought just to use scissors or a pen knife. Ask the kids they might be carrying a blade!! (joke, joke, honest). -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: 22 July 2009 14:58 To: Histonet Subject: [Histonet] histology for kids Hello All, My company is hosting an in-house science awareness day for local grade-school students. I would love to teach them about histology, but all of the demonstrations need to be done in our conference room (thus, nothing hazardous). Does anyone know of any house-hold dyes (grape juice, food coloring, beet juice, etc) that would stain tissue elements on slides? I would like to bring down some deparaffinized tissues and stain them with something and throw a coverslip on (water-mounted) so that they can look at the tissue with a microscope. I will also bring some already prepared slides (wtih real stains) for them to look at. Any ideas? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] nuclear bubbling
Sub optimal fixation and as Rene said; drying off at high temperatures. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: 15 July 2009 20:45 To: Histonet Subject: [Histonet] nuclear bubbling Has anyone experienced nuclear bubbling on prostate biopsies? Joyce * CONFIDENTIALITY NOTICE * This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HTL
Do you want the blunt truth? There's a perception, even within the other disciplines in Diagnostic Labs, that BMS's in Histology (HistoTechs) are second rate Scientists. I know that's an inflammatory remark but I've battled with it for years. Pharmacists, Physiotherapists, Ots, Audiologists and Speech Language Therapists run Clinics treat Patients and are 'clinical'. The perception is that 'scientists' are not clinical and before we get appreciated for that we probably need to run Clinics ourselves but how do Histotechs/ BMS's achieve that? In the UK scientific staff are slowly doing that with Anticoagulant Clinics, with advanced dissection and the reporting of cervical smears after achieving the appropriate level of qualification. I'm hoping one day that the 'glass ceiling' will be taken off the Path Labs and that a scientist will, after obtaining his/ her degree, Masters (or PhD), like the Clinical Scientists, obtain the MRCPath and then clinically lead a discipline. Only when we step from behind the skirts of the Medics will the sun shine on us. Does that help? Kemlo Rogerson MSc MIBiol CBiol DMS CSci FIBMS (I tried). -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shea's Sent: 15 July 2009 04:13 To: jaustin1...@gmail.com Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] HTL Michael, Ditto, very well stated. I too believe that our industry is under appreciated. Many new grads of today find a two year degree demeaning and wouldn't consider HT because of it. I don't understand how some professions like pharmacy physical therapy gain respect and grow to create 5 yr, 6yr 7yr programs. They are very well respected by the MDs and Hospital administration and have nice salaries to show for it. Why hasn't our field flourished? Jan, BS, HTL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] What percent of HTL's do not have a BS degree?
The histology world doesn't look for well qualified workers they look for cheap labor (SIC). I have heard more than one pathologist state that a monkey can do our job. See my other post. The retort ought to be that a Histology BMS/ Histotech can do yours!! A honest Pathologist once told me that a good Histotech could report 80% of what he did, you needed some medical knowledge to maybe report the next 15% or so, Pathologists with a speciality probably reported the next 2% or 3% and it took an expert to deal with the top few percent. He taught me Pathology of the skin and I was good at it; I naturally then became a Cytologist as there's no way, without a MRCPath, that I could ever report skin biopsies. A Gynaecologist friend of mine once told that the Pathologist/ Histotech (BMS) relationship was perceived by many of his colleagues to be the last bastion of prostitution. I never figured out who was the pimp!! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael Bradley Sent: 14 July 2009 21:50 To: Weems, Joyce Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] What percent of HTL's do not have a BS degree? HI all I am a rarity. I am an HTL with a Bachelors Degree. I got my HTL in the early 90s and I guess I was misguided because I thought it would open more doors for me than just an HT. I was sadly mistaken. After I passed my test I waited 9 months for a raise and promotion (which was just a greater title) and when I got my raise so did 2 other employees that didn't even have or try for their certification. I spent many nights and weekends studying and doing my stains for the test. I am proud of my accomplishments. It is a shame that our industry does not reconize the difference between HT and HTL. A few years back I was working as a traveling histotech and when I tried to get a permanent position no one wanted to hire me because I was over qualified by having over 15 years experience and a HTL certification. I worked hard to no avail. The histology world doesn't look for well qualified workers they look for cheap labor. I have heard more than one pathologist state that a monkey can do our job. I have also worked in a lab where they would hire someone with a GED to cut slides. A career in histology is for the most part a dead end and there is no future. As long as our industry doesn't respect education and experience there will be less and less histotechs and the quality of the slides will suffer which in turn will bring down patient care. Just my 2 cents. MB proud HTL On Tue, Jul 14, 2009 at 3:37 PM, Weems, Joyce jwe...@sjha.org wrote: Honey... You are a mere child! There are some of us that have been in the business for 40+ years. I missed the grandfather approach by 7 mo - time that I didn't work moving from place to place with my military ex-husband. But I did finally get the degree and do the exam. But we're still around. And I'll probably be working till I'm 100!!! J:) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Tuesday, July 14, 2009 15:16 To: Feher, Stephen Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] What percent of HTL's do not have a BS degree? Hi Steve, I've got no statistics to offer you...just an observation. I would say that finding an HTL, without a Bachelor's degree is akin to the proverbial needle in a haystack. Anyone that obtained their HTL, if/when they could be grandfathered in, is likely to be retired or close to it. First of all, most folks that went the OJT route for certification were eligible to sit for the HT only (to my knowledge). I've never met anyone with an HTL that did not have a Bachelor's as a pre-requisite. I've been doing histology for ~25 years. I've met people from all over the country and various parts of the world. Truth is there isn't an abundance of HTLs out there. Unlike the Medical Lab world, with the basic differences between MTs and MLTs, anatomic path does not exactly mirror that with the HTL and HT. It's true the MT and HTL both require a Bachelor's, but responsibilities in most labs, etc., generally do not hinge on someone being an HT vs. an HTL. A person like myself is probably more common (Bachelor's and an HT). Unless you know of someone in particular; that you want to hire, with an HTL without a Bachelor's, I wouldn't waste time trying to justify it. I guess the bottom line is if you want an HTL, that person will almost assuredly have a Bachelor's. If you want to hire someone without a Bachelor's that is certified (HT) you'll have better luck. I think having an HTL is a great thing. I honestly have never pursued it (though eligible) as the circumstances of my career would not have rewarded me for doing so. As
RE: [Histonet] HTL
Some volunteer to stay 'behind the medics' as it is safe; some are kept there kicking and screaming (I'm hoarse). Medics are medics; it is a 'gentlemen's club' but non-Path medics are finding their position eroded by the Consultant Nurse and Consultant Physiotherapist. Pathologists are strenously opposing the idea of a Consultant Biomedical Scientist but bizzarely the Consultant Clinical Scientist is seen as OK. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anne van Binsbergen Sent: 15 July 2009 09:01 To: Kemlo Rogerson Cc: histonet@lists.utsouthwestern.edu; Shea's Subject: Re: [Histonet] HTL well said!! your statement: 'Only when we step from behind the skirts of the Medics will the sun shine on us' deserves dissection (pardon the pun) are we volutarily 'behind the medics or are we conveniently 'kept' there by those same medics medics=pathologists (some exceptions) where i come from most of these 'medics' are running the (very lucrative) private labs and the techs are kept 'lean and hungry' - they are 'worker bees'' grateful for work and paid a pittance. i once voiced my desire to take unpaid leave in order to study further and was refused time off for this, on the basis that i would then cost more to employ!!! i have a 4 year diploma (now called a BTech degree) - i am licensed as a Medical Technologist with Cell Path Speciality. i am neither an HT or an HTL. i have 30 years experience and have been supervising/managing AP labs for over 15 years but because i dont have a degree i would most likely have a hard time finding employment in the USA or Canada - your loss guys. its not what you call it its how you apply what you know - having a degree does not make you a good tech. flame away!! AnnieinArabia (out of Africa) 2009/7/15 Kemlo Rogerson kemlo.roger...@waht.swest.nhs.uk Do you want the blunt truth? There's a perception, even within the other disciplines in Diagnostic Labs, that BMS's in Histology (HistoTechs) are second rate Scientists. I know that's an inflammatory remark but I've battled with it for years. Pharmacists, Physiotherapists, Ots, Audiologists and Speech Language Therapists run Clinics treat Patients and are 'clinical'. The perception is that 'scientists' are not clinical and before we get appreciated for that we probably need to run Clinics ourselves but how do Histotechs/ BMS's achieve that? In the UK scientific staff are slowly doing that with Anticoagulant Clinics, with advanced dissection and the reporting of cervical smears after achieving the appropriate level of qualification. I'm hoping one day that the 'glass ceiling' will be taken off the Path Labs and that a scientist will, after obtaining his/ her degree, Masters (or PhD), like the Clinical Scientists, obtain the MRCPath and then clinically lead a discipline. Only when we step from behind the skirts of the Medics will the sun shine on us. Does that help? Kemlo Rogerson MSc MIBiol CBiol DMS CSci FIBMS (I tried). -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shea's Sent: 15 July 2009 04:13 To: jaustin1...@gmail.com Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] HTL Michael, Ditto, very well stated. I too believe that our industry is under appreciated. Many new grads of today find a two year degree demeaning and wouldn't consider HT because of it. I don't understand how some professions like pharmacy physical therapy gain respect and grow to create 5 yr, 6yr 7yr programs. They are very well respected by the MDs and Hospital administration and have nice salaries to show for it. Why hasn't our field flourished? Jan, BS, HTL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Anne van Binsbergen (Hope) Abu Dhabi UAE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Help With Hemo Fading
I agree.. Also you're not storing them in sunlight are you? Silly question I know. Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 02 July 2009 20:28 To: histonet@lists.utsouthwestern.edu; sr...@aol.com Subject: Re: [Histonet] Help With Hemo Fading The fading most probably is caused by acid in the permanent slide, probably because the sections were passed through the alcohols very quickly after the acid differentiation, or they stayed little time in tap water after differentiation or no bluing agent was used. It is unlikely that the mounting medium is acidic, although that could also be the cause also. An acid environment over the cover slipped section is the most probable culprit for the henatoxylin fading. Check the staining protocol. René J. --- On Thu, 7/2/09, sr...@aol.com sr...@aol.com wrote: From: sr...@aol.com sr...@aol.com Subject: [Histonet] Help With Hemo Fading To: histonet@lists.utsouthwestern.edu Date: Thursday, July 2, 2009, 2:42 PM Hello everyone, ? We are having problems with short-term hematoxylin fading and loss of detail. The pathologist is freaking out! I've seen hemo fade over a long period of time but not in a matter of a few months. Slides from one year ago are really bad. ? I've been out of the business for a number of years and in the interim much has changed including reagents. These are GI tract biopsies processed by microwave. ? Any thoughts at all? ? Thanks! Marg ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] signs of good perfusion
That's cruel; how would you like it? Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of raghul Sent: 30 June 2009 05:03 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] signs of good perfusion Dear Dzung, I wonder whether the perfusion would be good in an euthanized animal with the circulation arrested. It would be better to try it under deep anesthesia. Certainly perfusion would be better Regards Raghul RCC laboratories India Pvt. Ltd Hi Everyone!! I am perfusing CO2 euthanized 3 weeks old mice with PBS only. I am nicking the upper right atrium of heart to collect the gushed out blood and then perfusing through ventricle using a 21G butterfly needle and peristaltic pump. Sometimes I see the lungs swelling up and fluid comes out of mouth. Occasionally, I see the liver fade to light pink. Most of the time the paws become white. But the brain and spinal cord (my tissues of interest) are always white, and seem to have been perfused. I was wondering how to improve this to get more consistent good perfusions, and what signs should I look for to indicate good perfusion? Thanks much, Dzung NIAID -- Message: 5 Date: Mon, 29 Jun 2009 08:47:07 -0700 (PDT) From: Phyllis Thaxton dch...@yahoo.com Subject: [Histonet] Cardboard Paraffin Catchers To: Histonet@lists.utsouthwestern.edu Message-ID: 372922.65853...@web43506.mail.sp1.yahoo.com Content-Type: text/plain; charset=iso-8859-1 Does anyone remember the little cardboard microtome trays for paraffin waste? If so please email me the company that makes them. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL -- Message: 6 Date: Mon, 29 Jun 2009 12:07:04 -0400 From: Merced M Leiker lei...@buffalo.edu Subject: Re: [Histonet] Signs of good perfusion To: Thach, Dzung (NIH/NIAID) [E] thac...@niaid.nih.gov, histonet@lists.utsouthwestern.edu Message-ID: 1d8e958050abad54bd852...@cdywxp1931.ad.med.buffalo.edu Content-Type: text/plain; charset=us-ascii; format=flowed Live going pale is a good sign, your tissues of interest going pale is an even better sign, but fluid coming out of the mouth (or even the nose, or additionally, any kind of bloating or swelling in the animal) is a bad sign. You may not be able to get good perfusion (pale tissues) if this happens before your tissues turn pale, as the pressure is too high causing fluid to leak out of the vasculatureideally you want to push the blood out through the the hole you made in the right atrium, not through the walls of the vessels. at what rate do you perfuse? if this happens a lot slow it down. Hope this helps. --On Monday, June 29, 2009 11:31 AM -0400 Thach, Dzung (NIH/NIAID) [E] thac...@niaid.nih.gov wrote: Hi Everyone!! I am perfusing CO2 euthanized 3 weeks old mice with PBS only. I am nicking the upper right atrium of heart to collect the gushed out blood and then perfusing through ventricle using a 21G butterfly needle and peristaltic pump. Sometimes I see the lungs swelling up and fluid comes out of mouth. Occasionally, I see the liver fade to light pink. Most of the time the paws become white. But the brain and spinal cord (my tissues of interest) are always white, and seem to have been perfused. I was wondering how to improve this to get more consistent good perfusions, and what signs should I look for to indicate good perfusion? Thanks much, Dzung NIAID ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. -- Message: 7 Date: Mon, 29 Jun 2009 09:39:02 -0700 (PDT) From: Steven Coakley sjchta...@yahoo.com Subject: [Histonet] WI/MI To: Histonet@lists.utsouthwestern.edu Message-ID: 385293.93112...@web38204.mail.mud.yahoo.com Content-Type: text/plain; charset=iso-8859-1 I'm looking for a few
RE: [Histonet] Sheep Lung
Fixed or unfixed sheep lung? If fixed then you can perfuse with formalin from a header tank 1 metre above lung and then infiltrate with gelatine. Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Annette Featherstone Sent: 24 June 2009 13:52 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sheep Lung We will be cutting frozen sections on sheep lung and I was wondering if anyone has a method to produce good quality sections. Are you injecting anything in the lung prior to sectioning? Annette Featherstone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] hood for manual special stains
SKIN: Repeated contact can produce dermatitis (dryness and cracking) due to degreasing action. SKIN SENSITIZATION: Skin sensitization was not produced in any of 24 volunteers. There is one case report of a person developing an allergic skin reaction (contact urticaria) following exposure to xylene (unspecified composition) vapour. The person subsequently tested positive in a patch test. No information was provided regarding previous history of allergies. No conclusions can be drawn regarding the potential for xylene to produce allergic skin reactions, based on this single case report. NERVOUS SYSTEM EFFECTS: Long-term xylene exposure may cause harmful effects on the nervous system, but there is not enough information available to draw firm conclusions. Symptoms such as headaches, irritability, depression, insomnia, agitation, extreme tiredness, tremors, and impaired concentration and short-term memory have been reported following long-term occupational exposure to xylene and other solvents. This condition is sometimes generally referred to as organic solvent syndrome. Unfortunately, there is very little information available which isolates xylene from other solvent exposures in the examination of these effects. Other study deficiencies include inadequate reporting on the duration of exposure and the exposure levels, and poor matching of controls. In a recent study, 175 employees were exposed to an average xylene concentration of 21 ppm for an average of 7 years. Subjective symptoms such as anxiety, forgetfulness, inability to concentrate and dizziness were reported. Xylenes accounted for greater than 70% of the total exposure.) This study is also limited by factors such those described above. BLOOD EFFECTS: Historical reports sometimes associate xylene exposure with certain blood effects, including leukemia, which are now known to be caused by benzene. Uncontaminated xylene is not known to cause these effects. Reduced blood platelet counts were observed in 12 of 27 men exposed to xylene (unspecified composition) at a level up to 200 ppm. When exposure stopped, platelet counts returned to normal. There is insufficient information to draw any conclusions from this study. LIVER AND KIDNEY EFFECTS: A number of case reports and occupational studies have suggested that liver and kidney damage may result from long-term occupational exposure to xylene. However, it is not possible to attribute these effects directly to xylene exposure because generally there was exposure to other chemicals at the same time, particularly other solvents, and there was no information provided on the exposure levels or duration of exposure. In a recent study, 175 employees were exposed to a mean xylene concentration of 21 ppm for an average of 7 years. Liver and kidney effects were not reported. Xylenes accounted for greater than 70% of the total exposure. From Web Site. In the UK we have to limit exposure to both formalin and xylene. Most modern Labs have downdraught dissection tables and benches have back ventilation. Neither of these chemicals ought to be tolerated if you can smell them and exposure ought to be controlled. Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Love Sent: 24 June 2009 23:34 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] hood for manual special stains The hospital where I work just opened a new lab. The histology department is now combined with cytology and is made up of me, a second histotech, a cytology prep tech, and our cytotech supervisor. We didn't have a hood in the old lab, so we did our manual special stains on a workbench with weak, overhanging vents that looked like lamps. The one hood in our new department completely belongs to the cytology prep tech. She has her centrifuge and a lot of other equipment in it, and my supervisor does not want me or the other histotech to share it. Some of our workbenches have small vents built into the walls, but when I do my specials there, I still smell chemicals. Do most histotechs today use hoods to do manual special stains or am I being too wishful? _ Hotmail(r) has ever-growing storage! Don't worry about storage limits. http://windowslive.com/Tutorial/Hotmail/Storage
RE: [Histonet] microtome calibration
Have you tried altering the angle of the knife? Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dianne Holmes Sent: 02 June 2009 22:44 To: Histonet Subject: [Histonet] microtome calibration Has anyone had problems with the calibration of an A/O Spencer sliding microtome? I have used this one for years and now I am getting 'thick-thin' sections? I thought maybe the accumulation of oils in the mechanics of it have slowed things down. Can WD40 be applied without adverse effects? I certainly would not attempt opening up all the gears!! Any suggestions would be appreciated or if a medical equipment repairman is reading this - I really need this thing fixed. Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Recycling Formalin for Fresh Tissue
Why would you want to? Surely the time and energy needed to make sure that the recycled formalin was of the correct strength, was not infective and was at the correct pH and properly buffered would not make it cost effective. Bit like using a teabag twice; that's uncivilised!!! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of akemiat3...@yahoo.com Sent: 28 May 2009 17:14 To: Histonet Subject: [Histonet] Recycling Formalin for Fresh Tissue Good Morning Histo Land, I am asking all you out there to give me your input on recycIing formalin. I realize this has been discussed in the not too distant past, but this may be a little different situation. I realize some labs are recycling formalin to put on their tissue processors, but this is a totally different scenario. I have been a manager at my lab for only a month. Much to my surprise, I just found out yesterday that our lab is recycling formalin and shipping it out to one of our clients to put their FRESH SPECIMENS into. I just had a lengthy conversation with Robert Lott the day before yesterday, regarding pre-analytical protocol's effecting the test results of AFB. By the way, we were in full agreement. This formalin issue was unknown to me then. I realize that the pre-analytical, pre-analytical, pre-analytical, process effects the final out come. I need to present my case to our Director of Pathology why this is compromising patient care. Any and all responses are gladly welcome. Thanks, Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Ct. Los Gatos, CA 95032 Cell: 425.941.4287 W: E-Mail: aallison-ta...@apmglab.com P: E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cloudy formalin in vip tissue processor
That's a 'harsh' fixative; used it years and years ago as it mordants the haematoxylin. I think that I still stand by the harsh effects of that fixative on coagulating protein and that the culprit is precisely that; coagulated protein from using a additive fixative (as you know formalin is a coagulant, non-additive fixative, normally). Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Christine I. Braaten Sent: 22 May 2009 17:36 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cloudy formalin in vip tissue processor Thanks to all who have replied to my query. I probably should have mentioned that we use zinc formalin in our processor and as our primary fixative. I have spoken to both Sakura and Anatech (supplier of zinc formalin) and feel more confused. Anatech says it's a change in pH that causes the zinc to precipitate and that xylene will cause a whole lot more problems than a little cloudiness. Sakura thinks the zinc formalin is the problem. We clean the retort with the cleaning program every morning and make sure to dry the retort so I'm not sure how xylene would get into the formalin. If this brings up any other issues I would appreciate more feedback. Thanks CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Bone!
As I say I don't have experience of preservatives but question if the effects of ethanol (70%) over a long time may also 'overfix' the bone? Anyway 70% ethanol has perfectly preserved me!! Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: 22 May 2009 13:21 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Bone! Lets face it there is no really ideal medium for long term storage. However this depends on what techniques you want to carry out later and when. I agree that 10% NBF is a good fixative for bone, I would definitely not advise leaving it in this fixative for extended periods of time. The reason is that formalin continues cross linking. This means that many stains and immunohistochemistry may no longer be possible depending on the amount of time left in fixative. Also Kemlo is correct in that temporary formalin bonds can be broken by extended washing in watre. This only applies to the weak temporary bonds and not to the more stable bonds. The longer the fixing the greater the number of stable bonds. I would recommend having a mixture of 70% ethanol plus glycerin up to 10-20%. The glycerin will prevent any drying out of the solution over extended periods of tim. Barry From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson [kemlo.roger...@waht.swest.nhs.uk] Sent: Friday, May 22, 2009 2:24 AM To: Robert Edward Pogue Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Bone! Probably of all the fixatives 10% neutral buffered formalin is least likely to overfix and I've left tissues in it for years. Formalin, as you know, is one of those fixatives that you can wash out. There are preservatives, rather than fixatives, you could try but I don't have experience of those; my best advise is formalin but make sure that it is neutral and buffered. I think the effects of pH would be more damaging than the relatively 'soft' formalin fixation. Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Edward Pogue Sent: 21 May 2009 16:33 To: Kemlo Rogerson Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Bone! Thanks Kemlo... I thought of that, but was worried about over-fixation,- what do you think? Redward. -- In 10% neutral buffered formalin? Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Edward Pogue Sent: 21 May 2009 14:47 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bone! Friends, Can someone recommend to me how to store bones (juvenile rat femur) that I have fixed and decalcified, but am not yet ready to cut (they're for vibratome, so not embedded). Thanks! Redward. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cloudy formalin in vip tissue processor
hehehe, thanks... Only got it completely the wrong way around didn't I? Freida is correct, I think! Kemlo Rogerson e-mail kemloroger...@nhs.net mailto:kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From: Freida Carson [mailto:frei...@sbcglobal.net] Sent: 26 May 2009 13:38 To: Kemlo Rogerson Subject: RE: [Histonet] cloudy formalin in vip tissue processor I'm sorry, but formalin is an additive, non-coagulant fixative. I am not sending this through the histonet, but did want to correct you. I imagine others will also. Formalin adds on at the amino group. Freida Carson --- On Tue, 5/26/09, Kemlo Rogerson kemlo.roger...@waht.swest.nhs.uk wrote: From: Kemlo Rogerson kemlo.roger...@waht.swest.nhs.uk Subject: RE: [Histonet] cloudy formalin in vip tissue processor To: Christine I. Braaten cbraa...@cheshire-med.com, histonet@lists.utsouthwestern.edu Date: Tuesday, May 26, 2009, 6:16 AM That's a 'harsh' fixative; used it years and years ago as it mordants the haematoxylin. I think that I still stand by the harsh effects of that fixative on coagulating protein and that the culprit is precisely that; coagulated protein from using a additive fixative (as you know formalin is a coagulant, non-additive fixative, normally). Kemlo Rogerson e-mail kemloroger...@nhs.net http://us.mc825.mail.yahoo.com/mc/compose?to=kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu http://us.mc825.mail.yahoo.com/mc/compose?to=histonet-boun...@lists.uts outhwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu http://us.mc825.mail.yahoo.com/mc/compose?to=histonet-boun...@lists.uts outhwestern.edu ] On Behalf Of Christine I. Braaten Sent: 22 May 2009 17:36 To: histonet@lists.utsouthwestern.edu http://us.mc825.mail.yahoo.com/mc/compose?to=histo...@lists.utsouthwest ern.edu Subject: [Histonet] cloudy formalin in vip tissue processor Thanks to all who have replied to my query. I probably should have mentioned that we use zinc formalin in our processor and as our primary fixative. I have spoken to both Sakura and Anatech (supplier of zinc formalin) and feel more confused. Anatech says it's a change in pH that causes the zinc to precipitate and that xylene will cause a whole lot more problems than a little cloudiness. Sakura thinks the zinc formalin is the problem. We clean the retort with the cleaning program every morning and make sure to dry the retort so I'm not sure how xylene would get into the formalin. If this brings up any other issues I would appreciate more feedback. Thanks CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://us.mc825.mail.yahoo.com/mc/compose?to=histo...@lists.utsouthwest ern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://us.mc825.mail.yahoo.com/mc/compose?to=histo
RE: [Histonet] cloudy formalin in tissue processor
The old tissue processors used to have cloudy formalin to the point you'd get a layer at the bottom of the jars. If it is not contamination from the clearing fluids already suggested, could it not just be the fixed protein from the tissues that is held in suspension. I would have thought very bloody or very proteinaseous tissue would have these labile proteins fixed which would then fall out of solution; from memory the cold makes it worse. Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Christine I. Braaten Sent: 21 May 2009 17:42 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cloudy formalin in tissue processor Has anyone had a problem with their formalin getting cloudy in their tissue processor? We have a VIP tissue processor and just recently started having problems with cloudy formalin. Any suggestions would be appreciated. I checked the archives and couldn't find any articles on this particular subject. Thanks. CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Bone!
Probably of all the fixatives 10% neutral buffered formalin is least likely to overfix and I've left tissues in it for years. Formalin, as you know, is one of those fixatives that you can wash out. There are preservatives, rather than fixatives, you could try but I don't have experience of those; my best advise is formalin but make sure that it is neutral and buffered. I think the effects of pH would be more damaging than the relatively 'soft' formalin fixation. Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Edward Pogue Sent: 21 May 2009 16:33 To: Kemlo Rogerson Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Bone! Thanks Kemlo... I thought of that, but was worried about over-fixation,- what do you think? Redward. -- In 10% neutral buffered formalin? Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Edward Pogue Sent: 21 May 2009 14:47 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bone! Friends, Can someone recommend to me how to store bones (juvenile rat femur) that I have fixed and decalcified, but am not yet ready to cut (they're for vibratome, so not embedded). Thanks! Redward. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Gram AFB staining FNA smears
Agree; I guess the difference is that FNAC and Micro samples haven't been subjected to processing like the tissue block sections. The tissue stains have been shown to work on something that has been fixed, dehydrated, set in hot wax, cut, rehydrated and then stained. The FNAC and Micro samples may or may not have been fixed (although I concede air drying is a form of fixation) and the stains used on them have been shown to work. The logic that these techniques are interchangeable is not only flawed but (oxy)moronic. Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 06 May 2009 17:24 To: histonet@lists.utsouthwestern.edu; Angela Bitting Subject: Re: [Histonet] Gram AFB staining FNA smears The doc is wrong, otherwise your histology sections to be stained with Gram AFB should also be sent to micro. Perhaps you will not have to do them anymore. Your doc's reasoning is purely oxymoronic. René J. --- On Wed, 5/6/09, Angela Bitting akbitt...@geisinger.edu wrote: From: Angela Bitting akbitt...@geisinger.edu Subject: [Histonet] Gram AFB staining FNA smears To: histonet@lists.utsouthwestern.edu Date: Wednesday, May 6, 2009, 9:22 AM The subject of staining FNA smears with AFB and Gram stains in Histology vs in Micro came up today. Is there a reason that FNAs can't be stained the same way we stain our tissue sections? One of our docs was under the impression that it's not acceptable to use the staining methods we use in our Histology lab. I don't know what method Micro labs use, so I was hoping someone could shed some light on this subject for me. Thanks, as always, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] New HTL Program!
Congratulations from the UK. As you know we've had this in place for many years and resulted in the profession having a higher profile; I hope the rest will follow for you. Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: 07 May 2009 15:03 To: Sebree Linda A Cc: histonet@lists.utsouthwestern.edu; Kimberly Feaster Subject: Re: [Histonet] New HTL Program! This is great news!! Good luck and let's all wish this school success for many in years into the future. It sounds like a very good program. Pam Marcum UPENN Vet School New Bolton Center - Original Message - From: Sebree Linda A lseb...@uwhealth.org To: Kimberly Feaster kfeas...@hsc.wvu.edu, histonet@lists.utsouthwestern.edu Sent: Thursday, May 7, 2009 9:57:03 AM GMT -05:00 US/Canada Eastern Subject: RE: [Histonet] New HTL Program! This is great news for our field! If I were 30 years younger. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kimberly Feaster Sent: Monday, May 04, 2009 8:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New HTL Program! West Virginia University School of Medicine Histotechnology Program is now accepting applications for fall 2009 enrollment! The WVU Histotechnology Program is offered as an area of emphasis within the Medical Laboratory Science major. Students graduate with a Bachelor's of Science degree in Medical Laboratory Science. The first two years consist of a pre-professional curriculum and the last two years consist of the Histotechnology Program curriculum. The pre-professional curriculum can be completed at WVU main campus, one of the WVU regional campuses or any regionally accredited college or university. The Histotechnology Program curriculum is based at the WVU Robert C. Byrd Health Sciences Center. The first year consists of a didactic schedule focusing on routine laboratory procedures with incorporated laboratory sessions and the second year will focus on complex procedures with on-site and clinical rotations. Clinical rotations will be completed at the program's affiliated clinical laboratories located in West Virginia and Pennsylvania. If anyone would like more information or know someone who does, please contact: Kimberly Feaster Histotechnology Program Director 304-293-7628 kfeas...@hsc.wvu.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Destain Hematoxylon
From memory I found that it was better to give a good water wash or go into bicarb after destaining with acid/ alcohol as the acidity impacted on the staining if you went from acid/ alcohol directly into haematoxylin again (if you wanted to restain it). Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Histonet Alias Sent: 04 May 2009 22:47 To: V. Neubert Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Destain Hematoxylon Acid alcohol. It may not remove all of it but it will remove the majority. On Mon, May 4, 2009 at 5:32 AM, V. Neubert histonet.nos...@vneubert.comwrote: Hi, I want to destain hematoxylin (Shandon's Gill II). How to do that? Thanks in advance, V. Neubert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Al Ias HT(ASCP) Histology Manager Pathology Laboratory United States ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (canine) eosinophil IHC
As already stated a good HE will, but a bad one will show them even better; wash most of the E out of the HE and the eosiniphils tend to let go of it last. Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: 04 May 2009 20:41 To: histonet Subject: [Histonet] (canine) eosinophil IHC Hello all, I'm asking this question on behalf of a researcher here. Does anyone know of the availability of an antibody that reacts with eosinophils, and if so, do you know if it reacts with canine eosinophils? Thank you in advance. Jan Shivers Senior Scientist Pathology Teaching Program Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Lab Week-Histology Trivial or Fun Facts LONG OFF TOPIC
I can only say Pathology needs more kids like you. I've no idea what your saying but it looks very interesting... Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of koelli...@comcast.net Sent: 15 April 2009 05:02 To: Disher Lori Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Lab Week-Histology Trivial or Fun Facts LONG OFF TOPIC Hi in grad school taking microanatomy and pathology classes, 2 that I heard are this: The surface area of all the alveoli in the lungs of an adult is between 40-70 square meters. That seems reasonable in having a 40-70 square meter surface (where all gas exchange takes place) represent all the gas exchange in lungs. Have seen that figure numerous times so while can't test it, can believe it. The other one that I also can't test and is hard to believe is that the sum total length of all vessels (large small, artery vein down to every single capillary) in one adult measures about 100,000 kilometers (62,000 miles). Again there are many disparate medical and anatomical references so either all right or all wrong. The 2 micron sectioned egg I don't believe. (1) There are 25,400 microns in an inch. A 2 inch long egg is about 50,000 microns long. At 2 microns per section thats about 25,000 egg sections. Even is each section is 2 square inches (that's generous since each end isn't close to 2 squre inches in area), thats 100,000 square inches. At 1,296 square inches per square yard, that's about 40 square yards which is far short of a football field (100 yards x 53 yards). (2) If you calculate the volume of a solid rectangle covering a football feild that is 100 yards x 53 yards x 2 microns and of course converting all to yards or microns, the answer is a specific volume. If you take the volume of an ellipsoid which is four thirds times pi times a times b times c with a, b and c being the lenggth of the 3 axis of the ellipsoid, and using approximate measurements for the egg, I come up with far , far less volume in egg than in the rectangular solid covering football field. (3) This is a classical calculus definte integral washer problem. Whether this egg as an ellipsoid is scalene, oblate or prolate, integrating volume over the limits of integration gives me much, much less volume than is needed to cover a football field 2 microns thick. Have tried all 3 methods and converting everything to microns or yards using scientific notation. So 6 calculations. Everytime I come up somewhere close to the area of 2 micron slices covering approximately 1/100 of the football field. Unless my math is all wrong, or this is a humongous, enormous ostrich and not chicken egg. Ray Raymond Koelling PhenoPath Labs Seattle, WA - Original Message - From: Disher Lori lori.dis...@hcahealthcare.com To: histonet@lists.utsouthwestern.edu Sent: Tuesday, April 14, 2009 12:12:40 PM GMT -08:00 US/Canada Pacific Subject: [Histonet] Lab Week-Histology Trivial or Fun Facts Hello, I was wondering if anyone has some histo trivial-fun facts to share for Lab Week? I remember a supervisor told me long ago that she was told while in training, that if you took a hard boilded egg and sectioned it at 2 microns you would have enough sections to cover a football field. Has anyone ever heard that one before? Can anyone contribute any others? We are trying to come up with some games for lab week. Lori ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Liver (control tissue for PAS/D)
I know this might be a stupid reply but as it's just for PAS/D can't you use animal liver? Why not pig's liver? Go to an slaughter house and get fresh pig's liver and bingo (I assume pig glycogen is the same as our's?)... You could fry the residual with onions, very nice. Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Putnam, Jodi Sent: 15 April 2009 13:56 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Liver (control tissue for PAS/D) Hi again. I was wondering if anyone knew of a good source for liver tissue for PAS/D control blocks. I am currently having to buy them and it is ~42.00 for 10 slides. I am hoping to find a better price than that as my doctor orders a fair amount of these. I don't have access to autopsy tissue and when I did there was a problem with autolysis. I work in a derm lab so I am limited. I have asked several of my former employers and so far no luck. Any help acquiring the tissue or just telling me about a better price with a different vendor would be great. I will be totally out of tissue within ~2 weeks (if I am lucky to have it last that long). Thanks and everyone have a great day. Thanks to everyone for info for manuals. I am so glad I signed up for the histonet. Jodi Jodi Putnam (HT,ASCP) Graves Gilbert Clinic Pathology Department 201 Park Street Bowling Green, KY 42102 (270) 393-2728 (voicemail) (270) 393-2795 Fax : (270) 393-2736 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Graves-Gilbert Clinic. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] FREEZY spray
Say thank you Uncle Kemlo!! Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: 08 April 2009 08:58 To: Bernie Taupin; Akemi Allison-Tacha; Jennifer MacDonald; Ingles Claire Cc: Histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: RE: [Histonet] FREEZY spray Hey, Would you believe that putting the word Taupin into my outlook rules actually works. Now every obnoxious post from Taupin actually disappears into my Junk folder and I do not have to read the drivel. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, 8 April 2009 3:38 PM To: Akemi Allison-Tacha; Jennifer MacDonald; Ingles Claire Cc: Histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] FREEZY spray Sorry, again, I'm confused... are you responding to me? I'm not the original poster... I never stated I had trouble with cracking, because, well, I don't. I'm Bernie Taupin, the King of Cryomicrotomy, Esq. From: Akemi Allison-Tacha akemiat3...@yahoo.com To: Jennifer MacDonald jmacdon...@mtsac.edu; Ingles Claire cing...@uwhealth.org; Bernie Taupin bernietau...@ymail.com Cc: Histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Sent: Wednesday, April 8, 2009 1:27:35 AM Subject: Re: [Histonet] FREEZY spray Sorry, I too noticed that the text looked weird then I did a cut and paste. I keep all the Data Sheets I created in my files, and this was just a portion of the Notes from the original Data sheet. I thought it might be helpful because you stated you had difficulty with cracking. You did not mention using isopentane. Some people immerse the tissue straight into liquid nitrogen, which could cause freezing artifacts. Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Court, Los Gatos, CA 95032 Cell: (425) 941-4287 E-Mail: akemiat3...@yahoo.com --- On Tue, 4/7/09, Bernie Taupin bernietau...@ymail.com wrote: From: Bernie Taupin bernietau...@ymail.com Subject: Re: [Histonet] FREEZY spray To: Akemi Allison-Tacha akemiat3...@yahoo.com, Jennifer MacDonald jmacdon...@mtsac.edu, Ingles Claire cing...@uwhealth.org Cc: Histonet@lists.utsouthwestern.edu, histonet-boun...@lists.utsouthwestern.edu Date: Tuesday, April 7, 2009, 10:11 PM I use isopentane suspended in liquid nitrogen, too. sorry if this sounds curt, but due to your cut-and-pasting, im not entirely sure what point youre trying to get at...? From: Akemi Allison-Tacha akemiat3...@yahoo..com To: Jennifer MacDonald jmacdon...@mtsac.edu; Ingles Claire cing...@uwhealth.org; Bernie Taupin bernietau...@ymail.com Cc: Histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Sent: Wednesday, April 8, 2009 1:03:04 AM Subject: Re: [Histonet] FREEZY spray Bernie, I used to use the method below for FS on muscles at Emanual Hospital in Portland OR. Since we had such wonderful success, we incorporated this method for all FS. Years later, I developed the MATSSE (pH3.4) 1-Step Trichrome Stain Kit (Modified Trichrome Method for Muscle Biopsies Cut on Frozen Sections) for Biocare Medical. This kit has long since discontinued by the way...This came from Biocare's Data Sheet. NOTE:Muscle tissue should be suitably obtained and frozen within 30 minutes after excision. The usual method of freezing is to first mount a transverse section of the muscle on a chuck using 10% tragacanth gum or equivalent commercial frozen section mounting media as the adhesive. The chuck with the mounted specimen is then immersed in prechilled isopentane until frozen. Isopentane is precooled when a container of the substance is placed into liquid nitrogen. At a temperature of -160° C, the isopentane has a slightly syrupy consistency. Care
RE: [Histonet] Controls needed!
It's just a thought, can't you get the control from your Microbiology Lab? You just need to make a cell block out of the bacteria that they've grown; I'm not aware that Hpylori has to go through a human system before you can demonstrate it. Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hofecker, Jennifer L Sent: 08 April 2009 13:14 To: Knutson, Deanne; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls needed! Hi Deanne, Have you checked with the NSH Control Tissue Bank? The form to request tissue is online on the NSH web page (www.nsh.org). The bank is run by the Quality Control committee in conjunction with the IHCRG. It is a free service to NSH members. You may contact the committee chair, William DeSalvo at wdesalvo@hotmail.com with any specific questions. Have a great week. Jennifer L. Hofecker HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph 615.343.0083 fax 615.343.7089 -Original Message- From: Knutson, Deanne [mailto:dknut...@primecare.org] Sent: Tuesday, April 07, 2009 3:06 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Controls needed! We are looking for H. Pylori control tissue and also GMS/fungus control tissue. Is there anyone out there that might have extra to share? We have good GRAM control blocks that we would be happy to exchange. Please let me know if you can help us out. Thank you! Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center Bismarck, N. Dak. 58506 701-530-6730 Fax 701-530-6735 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Histology
I think one of the things that I've learnt by leaving the world of Histology and dabbling (not too well) in the other disciplines is that there is no such thing as exacts. The maximum and minimum times for fixation for tissue depends on the tissue, the species, the temperature, the manufacturer of the fixative and, ah yes... The time of fixation. I think you need to do what the chemists do and control the reaction; fixatives alter proteins and the rate of reaction is dependent that which I've already said. Why concentrate on one variable? Surely the time taken to fix is just one of the variables and you need to control them all. As in all chemical reactions the time taken is dependent on all the others and you need to determine how that fixative works in your Lab, at your ambient temperature, with your manufacturer of fixative on the species of the tissue you are fixing. Multiple blocks of the same tissue, fixed for differing times and processed the way after being fixed with the same fixative; you can't go wrong. If I've been helpful then I apologise (g). Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: 07 April 2009 00:16 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology I hate to bring up histological technique for a change of pace but... Could we get some consensus of opinion as to maximum, minimum and optimal fixation times for different tissues? This is assuming that tissues will be fixed in buffered formalin at room temperature and processed to wax with a standard technique in a processor. This would also require a standard thickness for each tissue type. If there are students out there looking for projects this might seem to be suitable, as a few tissues only could be examined at one time. I know that several papers have been published about fixation in formalin but can't bring to mind any that deal with this aspect of the topic. If there are any students out there who would like a summary of fixation in general I will be happy to email it to them. Barry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Histonet Digest, Vol 65, Issue 14
Can I just say that you are giving him more importance than he's worth and you are perpetuating it? Obviously he must feed off your responses so stop being manipulated; just ignore or delete or make an Outlook rule, as I have, that his e-mails go to trash!! Bernie, Bernie, where are you? I can't see you. Well I can cos people him replying to you and the craps at the bottom of the e-mail. My point so stop inflicting me on him, please! Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez Sent: 07 April 2009 14:45 To: MKing; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 65, Issue 14 I second it!!! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of MKing Sent: Tuesday, April 07, 2009 6:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 65, Issue 14 a motion is hereby offered to have the active members of histonet vote this troll off the island, or at least request that the list moderator do so. this person has proven to have absolutely nothing of value to contribute to this group, and is only intent on disrupting it. second? - Message: 16 Date: Mon, 6 Apr 2009 15:43:07 -0700 (PDT) From: Bernie Taupin bernietau...@ymail.com Subject: Re: [Histonet] Nice one Kemlo To: histonet@lists.utsouthwestern.edu Message-ID: 453473.65848...@web43516.mail.sp1.yahoo.com Content-Type: text/plain; charset=iso-8859-1 Instead of spitting your venom try to learn Sort of like you might consider learning correct English? I mean, its one thing if its not your first language, but despite how many people on here called you kindly and helpful, I find it perplexing that you feel compelled to call me a piece of ignorant, and TWICE, no less. I do not speak French. This list is not in French. Ergo, my awareness (or lack thereof) of proper French diction has absolutely nothing at all to do with anything... aside form providing you a vehicle to call me names and lash out. If you have something to say, say it. The simple fact that you do not like me does not give you- or anyone else- wholesale right to insult me or call me names. You should be ashamed of yourself, Rene. I never expected such antisocial behavior from you, and frankly, when you behave so regrettably, I feel no compulsion to learn anything form you! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Job opportunity in Cambridge, MA
Try: Apply this rule after message arrives with Bernie Taulin in the senders address Delete it Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of michelle.bro...@novartis.com Sent: 07 April 2009 15:01 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Job opportunity in Cambridge, MA Hello all! We are a small pathology group within a large, Cambridge, MA based organization looking for a histologist. Our main function is to support drug development in a preclinical setting. The work would involve basic histology, IHC, and special staining on rodent tissue. Our ideal candidate is self-motivated, able to work independently but also as a team player, enthusiastic, and able to multitask. If that sounds like you, please visit our job posting at: http://www.novartis.com/careers/job-search/brassring/index.shtml and search for Job ID: 48106BR. We hope to speak with you soon! Regards, Michelle Broome Team Leader Preclinical Safety, Pathology Translational Sciences Novartis Institutes for BioMedical Research, Inc. 250 Massachusetts Avenue Cambridge, MA 02139 USA Email : michelle.bro...@novartis.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Getting rid of pests
Or Bernie Taupin... Even. Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: 07 April 2009 15:16 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Job opportunity in Cambridge, MA Try: Apply this rule after message arrives with Bernie Taulin in the senders address Delete it Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of michelle.bro...@novartis.com Sent: 07 April 2009 15:01 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Job opportunity in Cambridge, MA Hello all! We are a small pathology group within a large, Cambridge, MA based organization looking for a histologist. Our main function is to support drug development in a preclinical setting. The work would involve basic histology, IHC, and special staining on rodent tissue. Our ideal candidate is self-motivated, able to work independently but also as a team player, enthusiastic, and able to multitask. If that sounds like you, please visit our job posting at: http://www.novartis.com/careers/job-search/brassring/index.shtml and search for Job ID: 48106BR. We hope to speak with you soon! Regards, Michelle Broome Team Leader Preclinical Safety, Pathology Translational Sciences Novartis Institutes for BioMedical Research, Inc. 250 Massachusetts Avenue Cambridge, MA 02139 USA Email : michelle.bro...@novartis.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Nice one Kemlo
I think the benefit of getting older SHOULD be that one gets wiser. To get old without acquiring wisdom is a waste. One of those wisdoms is to know when a conversation has ended. or when there's no point due to the immaturity or lack of insight of some of the audience. You can use that Bernie, to stick at the end of your e-mails as a 'wise saying' (g). Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: 06 April 2009 05:28 To: rjbu...@yahoo.com; histonet@lists.utsouthwestern.edu; alan taylor Subject: Re: [Histonet] Nice one Kemlo 1. How is anyone supposed to know your gender online when you have such an androgynous name? 2. what does your gender have to do with this in the first place? gender equality is gender equality. but, not surprisingly, none of the feminists on this list have come out to say that. though i bet some would if i said something sexist, eh? From: Rene J Buesa rjbu...@yahoo.com To: histonet@lists.utsouthwestern.edu; alan taylor aj.tay...@blueyonder.co.uk Sent: Saturday, April 4, 2009 1:01:36 PM Subject: [Histonet] Nice one Kemlo Thanks to Kenlo and Alan for your nice words, only a detail though: I am a HE and not a SHE that just turned 75 on April First, I am a legitimate April Fool and I intend to keep doing what I am doing for as long as I can. René J. --- On Sat, 4/4/09, alan taylor aj.tay...@blueyonder.co.uk wrote: From: alan taylor aj.tay...@blueyonder.co.uk Subject: [Histonet] Nice one Kemlo To: histonet@lists.utsouthwestern.edu Date: Saturday, April 4, 2009, 12:42 PM Nice one Kemlo: Rene has always impressed me with the depth and breadth of her obviously great knowledge and skill in the practice of our humble art. Long may she continue to do so. As a histo group we should really strive to help each other and share our many and unique skills and applied techniques. A Giemsa squash prep for seeking Entamoeba, as described, sounds very appropriate to me. Come on guys, there has been a lot of knocking and critiscm of colleagues online lately. Now is the time to stop. Alan Taylor BSc(Hons), FRMS. Microtechnical Services Exeter. Devon. England. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: RE: RE: [Histonet] IHC on paraformaldehyde-fixed
Isn't petrol toxic? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Don’t be afraid to take a big step when one is indicated. You can’t cross a chasm in two small jumps. --Buckminster Fuller This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Tony Henwood Sent: 07 December 2008 22:06 To: [EMAIL PROTECTED]; Reuel Cornelia; histonet; [EMAIL PROTECTED]; Jan Shivers Subject: RE: RE: RE: [Histonet] IHC on paraformaldehyde-fixed Tf, In answer to you email: No I do not carry toxic liquid in my car. But does the PFA powder dissolve easily? My experience is that you need to make the solution alkaline then heat it. I never add methanol to my 10% neutral buffered formalin (it is buffered and diluted ie 10%). The risk of polymerisation of the formalin (since it is diluted) and formic acid formation (since it is buffered) is greatly reduced. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: tf [mailto:[EMAIL PROTECTED] Sent: Saturday, 6 December 2008 3:01 PM To: Reuel Cornelia; Tony Henwood; histonet; [EMAIL PROTECTED]; Jan Shivers Subject: Re: RE: RE: [Histonet] IHC on paraformaldehyde-fixed you want to carry a bottle of toxic liquid on your car? or you will take a box of powder that can dissolve into useful solution easily? You have to add methanol in 10% formalin 4% formaldehyde, rather 4% paraformaldhydePFA is methanol free..it's very important. 2008-12-06 tf 发件人： Reuel Cornelia 发送时间： 2008-12-06 00:09:36 收件人： Tony Henwood; [EMAIL PROTECTED]; histonet; [EMAIL PROTECTED]; Jan Shivers 抄送： 主题： RE: RE: [Histonet] IHC on paraformaldehyde-fixed I have been curious about this discussion. we used 4% paraformaldehyde for smaller biopsies only because it has a faster penetration to tissue than 10% formalin. In all my IHC that I have done. I observe that doing an IHC with 4% paraformaldehyde does not necessarily need antigen retrieval in comparison to 10% formalin either it will be human or animal tissue but this depends on how long was it fix, our 4% paraformaldehyde we fix smaller biopsies like nerve,muscle, skin for 6 to 12 hrs. and for formalin it is 12 to 48 hours or more. Maybe you can comment on the effect on this to tissue if you say you will use 4% paraformaldehyde for storage and transportation. Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 Tony Henwood [EMAIL PROTECTED] 12/04/08 9:29 PM tf wrote: I DO believe that one reason some people use 4% PFA rather 10% formalin is that PFA is a bit more stable, both for storage and transportation~~~. I have not heard this before. Do you have a reference for this? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: tf [mailto:[EMAIL PROTECTED] Sent: Friday, 5 December 2008 2:11 PM To: Tony Henwood; [EMAIL PROTECTED]; Jan Shivers; histonet Subject: Re: RE: [Histonet] IHC on paraformaldehyde-fixed the basic principles are the same for most cross-linking fixatives and induce similar bonds the difference you observed between may due to any other variability, or the co-fixative you used. I DO believe that one reason some people use 4% PFA rather 10% formalin is that PFA is a bit more stable, both for storage and transportation
RE: [Histonet] can anyone tell me...
So it is 10% formol alcohol in 70% alcohol; wonder what the other 20% is? Water? Alcoholic fixatives as a genre tend to overharden on standing but I guess fatty tissue would benefit. Why would anyone use something you don't actually know what it is composed of? -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 12 November 2008 13:48 To: [EMAIL PROTECTED]; histonet@lists.utsouthwestern.edu; Heath, Nancy L. Subject: RE: [Histonet] can anyone tell me... Found this on Histosearch. Also, when I was in a hospital lab we used it all the time on breast and any fatty tissues with great success. http://www.histosearch.com/histonet/Jan00/Penfix.html Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 [EMAIL PROTECTED] -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Rene J Buesa Sent: Wednesday, November 12, 2008 8:42 AM To: histonet@lists.utsouthwestern.edu; Heath, Nancy L. Subject: Re: [Histonet] can anyone tell me... Nancy: Well this is a different question altogether, you are wondering about if you should use Penfix versus NBF (you should never use unbuffered 10% formalin). Penfix is a commercial mixture of less than 10% formalin + methanol + ethanol + 2-propanol (3 different alcohols) in undisclosed (proprietary amounts) never evaluated independently (meaning that the only evaluation was done by the manufacturer). It has been said (anecdotalal information) that overfixes and dries small biopsies, something understandable due to the alcohols it contains. Since alcohols and formalin fix tissues in very different ways I personally do not see any advantage (and I could think on some disadvantages) of this combined fixation. I personally would NOT use it. Now it is after you.René J. --- On Wed, 11/12/08, Heath, Nancy L. [EMAIL PROTECTED] wrote: From: Heath, Nancy L. [EMAIL PROTECTED] Subject: [Histonet] can anyone tell me... To: histonet@lists.utsouthwestern.edu Date: Wednesday, November 12, 2008, 6:40 AM Hi Everyone :) Thanks to all for answering my question about why you would put bone in PennFix before decal :) Maybe I should have written the question a bit differently. I know bone is to be well fixed before decal...I just wanted to know why someone would put bone in Pennfix versus 10% neutral buffered formalin??? Is there any difference or extra benefits with PennFix?? Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
