Re: [Histonet] HTL Certification

[Rathborne, Toni via Histonet]

Hi Diana,

We have had two students complete the program at University of North Dakota. 
Both passed their certification exam the first time. You can get more 
information from this link.

 https://med.und.edu/histotechnology/admission-requirements.html

Best of luck finding a program that works for you!

Toni





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-Original Message-
From: Diana Martinez-Longoria via Histonet 
Sent: Thursday, June 27, 2024 12:48 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] HTL Certification


*** This is an External Email ***

Good day all,


I have a question that hopefully I get some guidance on. I have an HT 
certification, but I wanted to try to study for the HTL certification, but it 
has been a very long time since I had to study for the boards therefore, I was 
wondering if you guys that are so knowledgeable can give me guidance on how to 
pursue my endeavor. I am a type of person that needs structure and an outline 
on how to study, so preferably I would need like an online school that can help 
me for the HTL.  Any recommendations?


Thank you,

Diana Martinez-Longoria

El Centro Regional Medical Center

Lead Histotechnician (ASCP)cm, B.S, A.S

Laboratory – Pathology Department
1415 Ross Ave | El Centro, CA  92243

(: 760.339.7267  | *: diana.martinez-longo...@ecrmc.org



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Re: [Histonet] IHC background

[Rathborne, Toni via Histonet]

We have not encountered a covertile artifact with our Bond III, which we've had 
for over 10 years. It may be that the covertiles need to be replaced or cleaned 
more, but we have not experienced this. We also place a control tissue at the 
top of the slide, and often have large patient sections on the lower portion. 
Rene had some good suggestions. I would also call Leica to see if they can 
offer suggestions over the phone. Their technical support team has been very 
helpful over the years.
Best of luck.




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-Original Message-
From: Regan Fulton via Histonet 
Sent: Thursday, September 29, 2022 3:42 PM
To: RENE MORALES 
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC background


*** This is an External Email ***

Hi,

The artifact you are seeing is a well-known artifact of the Leica covertile
design.
It does limit the available footprint for placing samples.

Hope this helps.

Regan



Regan Fulton, M.D., Ph.D.
CEO and Co-Founder
Array Science, LLC
475 Gate 5 Road, #100
Sausalito, CA 94965
(415) 577-7360



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On Thu, Sep 29, 2022 at 12:37 PM RENE MORALES via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Paula,
> Regardless of the position of the tissue: control/ patient ‘s tissue, most
> likely is the  distribution of the reagents during staining, isn’t
> adequate. Syringe, staining  reagents expiration, calibration, etc.,   Have
> you contacted service from Leica? Hope the solution is found,
> Thanks,
>
>
> Sent from my iPhone
>
> > On Sep 29, 2022, at 12:00, Paula via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
> >
> > Hello everyone,
> >
> >
> >
> > We have the Bond III and we ran an S100.  We placed 2 serial sections
> > vertically on the slide with the control tissue at the top for staining.
> The
> > patient's section at the top underneath the control tissue had background
> > staining, while the section on the bottom of that had no background
> > staining.  Can someone determine why this would happen?
> >
> >
> >
> > Thank you in advance.
> >
> > Paula
> >
> > Bio-Path Medical Group
> >
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Re: [Histonet] Stainer comparisons

[Rathborne, Toni via Histonet]

Rene,

I have the information from the vendors as you suggested. What I'm looking for 
is opinions. If you (or anyone else on histonet) have experience with either or 
both of these stainers, I'd like to know what you feel are the best features. 
Also looking to see what features are not as well praised or useful. Which 
specials do you run, and how do they compare to what you were previously using? 
How did getting one of these stainers impact your day-to-day operations? Did 
the positives outweigh the negatives, if there were any.

Thank you,
Toni

-Original Message-
From: RENE MORALES  
Sent: Monday, August 15, 2022 2:10 PM
To: Rathborne, Toni 
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Stainer comparisons


*** This is an External Email ***

Toni, the complete information can be found on the manufacturer specifications 
for these auto stainers. The only factor to be solved, no matter what 
instrument you will be selecting, is that you keep close monitoring of the 
service required for them. Important to be on the correct TAT.
Other factors are influencing the good results as well,
Thanks for sharing,
René Morales

Sent from my iPhone

> On Aug 15, 2022, at 07:23, Rathborne, Toni via Histonet 
>  wrote:
>
> 
> Good morning all,
>
> We're at the point where we are looking at replacing our H stainer, and I'm 
> wondering what opinions are out there about the Leica HistoCore Spectra ST - 
> High Throughput Routine & Special Stainer and the Sakura Prisma Plus Special 
> Stainer Configuration. What specials do you run on these stainers? Does this 
> significantly increase the TAT of the H slides? Overall, what do you like 
> or not like about either?
>
> Thank you,
>
> Toni
>
>
> 
>
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[Histonet] Stainer comparisons

[Rathborne, Toni via Histonet]

Good morning all,

We're at the point where we are looking at replacing our H stainer, and I'm 
wondering what opinions are out there about the Leica HistoCore Spectra ST - 
High Throughput Routine & Special Stainer and the Sakura Prisma Plus Special 
Stainer Configuration. What specials do you run on these stainers? Does this 
significantly increase the TAT of the H slides? Overall, what do you like or 
not like about either?

Thank you,

Toni




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Re: [Histonet] Microwave Reproducibility

[Rathborne, Toni via Histonet]

I would suggest starting with a standard amount of water (50-500 ml) to be 
tested at predetermined intervals according to your policy. Heat the water and 
record the temperatures at a minimum of 3 different times. Make sure that you 
are using a certified thermometer. From one test cycle to the next, you can 
note the deviation from your initial testing, and show reproducibility this way.




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-Original Message-
From: Willis, Donna G via Histonet 
Sent: Thursday, April 14, 2022 9:36 AM
To: Lora Jauregui ; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microwave Reproducibility


*** This is an External Email ***

What brand/type of microwave are you using?

Donna Willis
Anatomic Pathology Manager
Baylor Scott Health
Baylor University Medical Center
3500 Gaston Ave|Dallas, Texas 75246
214-820-2465 office|214-725-6184 mobile



-Original Message-
From: Lora Jauregui via Histonet 
Sent: Thursday, April 14, 2022 8:26 AM
To: histonet@lists.utsouthwestern.edu
Subject: {EXTERNAL} [Histonet] Microwave Reproducibility


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Any recommendations on a protocol for testing for reproducibility on our 
microwave and documenting it for CAP?  We are a new lab and just setting up 
equipment and such. We only use it to heat water and to heat silver solution 
for our GMS stain.
Thank you

Sent from 
Mail for Windows

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Re: [Histonet] slide printers

[Rathborne, Toni via Histonet]

Jessica,

We did this successfully. At one time, our cassette printers (Leica IPC) were 
not interfaced either, but we were able to use Leica's software and enter the 
cassette identifiers, and that information was able to be saved in the 
bar-code, which also printed on the cassette. Patient names were not printed, 
only the accession number/letter.
We have the SlideMate Pro printers, and before they were interfaced, we were 
able to scan the barcode on our blocks and they would print the information 
obtained from there. The SlideMates were also able to be programmed so that the 
hospital name was printed at the bottom of each slide.
So it can be done, but only if your blocks are bar-coded.

Toni




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-Original Message-
From: P Sicurello via Histonet 
Sent: Friday, January 14, 2022 10:06 AM
To: Piche, Jessica 
Cc: Histonet 
Subject: Re: [Histonet] slide printers


*** This is an External Email ***

Hi Jessica,

Slide writers don't have to be linked to a LIS but without that you really 
don't save any time. All the information will be typed in manually.

On the plus side-you don't have to worry about trying to read someone's hand 
writing if you use a slide writer.

Sincerely,

Paula Sicurello

Sent from my iPhone

> On Jan 14, 2022, at 5:35 AM, Piche, Jessica via Histonet 
>  wrote:
>
> Good Morning,
>
> Happy Friday Everyone.
>
> I was wondering what people are using/recommending for slide printers at the 
> microtome? Do they all have to be connected to an LIS?
>
> Thank you,
>
> Jessica
>
> Jessica Piche, HT(ASCP)
> Histology Team Leader
> Waterbury Hospital Histology Laboratory
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Re: [Histonet] microtome blades and wax

[Rathborne, Toni via Histonet]

Jessica,

What has Leica said about the problem? I have always found them to be 
responsive and able to provide suggestions. This would be especially true with 
a new instrument, still under warranty. We have never had any inconsistencies 
with our Autocut, which was purchased last spring.

Toni




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-Original Message-
From: Piche, Jessica via Histonet 
Sent: Friday, January 14, 2022 8:43 AM
To: Histonet 
Subject: [Histonet] microtome blades and wax


*** This is an External Email ***

Good Morning,

We have been using Leica Histocore Autocut microtomes for a few months and have 
been having inconsistency with cutting on them. We have tried so many different 
blades, high and low, and have tried changing the blade angle. Nothing is a 
perfect fix, and we feel like we are really having to work much harder to get a 
great section. We were wondering what everyone else uses. I was also wondering 
if the wax could be an issue. We use EM-400 for embedding. I'd appreciate 
anyone's thoughts.

Thank you and have a nice weekend!

Jessica

Jessica Piche, HT(ASCP)
Waterbury Hospital Histology Laboratory
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[Histonet] Looking for vendor - 2-circle slides

[Rathborne, Toni via Histonet]

Our current vendor for off-set double-cytofunnel slides has discontinued their 
product. If anyone uses these, I'd appreciate your vendor's information & part 
number.

Vendors are welcome to reply directly to me!

Thanks all,

Toni



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and destroy any printouts.




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Re: [Histonet] FW: Tissue disposal

[Rathborne, Toni via Histonet]


Stericycle will also take your blocks and slides when you're ready to discard 
them.

Toni 





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-Original Message-
From: Carol G Fields via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, January 29, 2020 2:05 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] FW: Tissue disposal


*** This is an External Email ***

FYI…_
From: Carol G Fields
Sent: Wednesday, January 29, 2020 8:32 AM
To: 'Eileen Akemi Allison' 
Subject: RE: [Histonet] Tissue disposal


Hi,
Stericycle takes care of all your waste, including decanting and disposal.  
They are all over the US..Health & Safety alone plus employee time will justify 
you using these people.  No one should still be doing this…
I’ve used this company at several hospitals. They are great.
Good luck,
Carole
MLKCH
Los Angeles, CA

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Stericycle is a compliance company that specializes in collecting and disposing 
regulated substances, such as medical waste and sharps, pharmaceuticals, 
hazardous waste, and providing services for recalled and expired goods. It also 
provides related education and training services, and patient communication 
services. Wikipedia
Stock price: SRCL (NASDAQ) $63.65 +0.11 (+0.17%)
Jan 29, 10:57 AM EST - Disclaimer
Headquarters: Lake Forest, IL
CEO: Cindy J. Miller (May 2, 2019–)
Number of employees: 23,200 (2017)
Founded: 1989
Subsidiaries: Shred-it, Stericycle Environmental Solutions, MORE



-Original Message-
From: Eileen Akemi Allison via Histonet 
[mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, January 29, 2020 4:25 AM
To: Histonet 
mailto:Histonet@lists.utsouthwestern.edu>>
Cc: mirnaiba...@umcelpaso.org
Subject: [Histonet] Tissue disposal

Good morning Histopeeps! After reviewing our Policy Procedure Manual, our 
manager is questioning the bags we are using for disposing our tissues after 
diagnosis is complete.  We are separating the tissue from the formalin by 
straining the excess formalin and neutralizing it.  We then put the tissue into 
“Large Biohazard Bags” and the EVS staff picks it up and disposes it.  My 
manager is wondering if the “Biohazard Bags" are appropriate.  Should the bags 
state “Tissue” on the them?  Are there any special disposal bags available?

While working for a GI Lab in Monterey, CA we put all our bottle and tissue in 
"Biohazard Bags" and a Hazardous Waste company picked up the waste products 
from our endoscopy department along with ours.  Any comments and suggestions 
would be greatly appreciated.

Thank you in advance,
Akemi Allison, BS, HT/HTL


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Re: [Histonet] Fwd: [HISTONET] Biocare

[Rathborne, Toni via Histonet]

I got this reply from my Biocare rep.

We are not going out of business   What happened is over a year ago we were 
acquired by a Venture group, which is a good thing. They infuse money and are 
making us stronger.





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and destroy any printouts.

-Original Message-
From: Colleen Forster via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, September 12, 2019 1:37 PM
To: Maria Cruz
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Fwd: [HISTONET] Biocare


*** This is an External Email ***

I'd love to know who actually told Michael this? I've had a company try
this on me as wellinterested to know if it is the same company.

C. Forster

On Thu, Sep 12, 2019 at 12:27 PM Maria Cruz via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Mr. Bradley:
> This is how awful rumors get started.  I’m sure that Biocare is not going
> out of business.  They’ve recently released a new stainer and last weak an
> IVD-labeled antibody for P16.  If a sales rep from another company told you
> this they are pretty unethical and could learn a few things from the people
> at Biocare.
> Maria
>
> -- Forwarded message --
> From: Michael Bradley 
> To: Histopeeps Histonet 
> Cc:
> Bcc:
> Date: Tue, 10 Sep 2019 00:28:08 +0200
> Subject: [Histonet] Biocare
>
> Hi all
>
> I heard a rumor that Biocare is going out of business. Can anyone confirm
> or deny this?
>
> Thanks.
> Sent using the mail.com
>  mail app
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>


--
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
B173 PWB  612-626-1930
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Re: [Histonet] Cerner AB

[Rathborne, Toni via Histonet]

I too would be interested in this information.

Toni Rathborne







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and destroy any printouts.

-Original Message-
From: deGuzman, Jose R via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, April 24, 2018 5:10 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cerner AB


*** This is an External Email ***

Hello Histonetters.

I'm looking for opinions from users of Cerner's Advanced Barcode labeling and 
Tracking Software. I'd like to know your experience working with the software, 
and if you've worked with another software.

Thank you.

Jose de Guzman

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Re: [Histonet] cutting test

[Rathborne, Toni via Histonet]

If a "practical" interview is permitted, I would also have the applicant embed 
some control tissue. You can tell a lot about a person's habits by observing 
them actually work. Can they talk and embed/cut at the same time, or do they 
stop working? Are they conscientious about specimen identification, 
cross-contamination, and quality? Or do they just speed through their work 
without paying attention, or are they too slow for your expectations?
Quite often these traits will carry over to other areas of a person's work 
habits, and you may find that this method is beneficial.

Toni




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reply e-mail, and permanently delete this e-mail and the attachments, if any, 
and destroy any printouts.

-Original Message-
From: Terri Braud via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, January 09, 2018 9:23 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] cutting test


*** This is an External Email ***

Please be sure to clear any use of a "cutting test" during the interview 
process with your institution's legal department.  If an interviewee should cut 
themselves, the liability could be steep.  If you have a clearly defined 
minimum cutting speed/quality, a better solution would be to hire the best fit 
for your lab, then, if they can't meet  the minimum requirements, then it's 
easy to let them go during the probationary period.  That's what it's for.
Terri

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal

Today's Topics:
   2. Suggested Requirements for new uncertified HTs (Erin McCarthy)
   3. Re: Suggested Requirements for new uncertified HTs (Jay Lundgren)
   4. Re: Suggested Requirements for new uncertified HTs
  (Sanders, Jeanine (CDC/OID/NCEZID))

Message: 2
Date: Mon, 8 Jan 2018 10:19:00 -0600
From: Erin McCarthy  Hi All,

I work at a Start-Up and I was our first hire. I have been practicing since
2011 when I got my certification. However, we are looking to expand, what would 
you suggest our baseline technical competencies be for a position that focuses 
exclusively on cutting and IHC. In an interview it is hard to access what a 
person is capable of, would it be acceptable for me to have applicants performs 
a cutting time test?
I am open to hiring new techs, but I want to make sure we are not hamstrung by 
a great interviewer with a lacking technical skill set.
Thanks!
Erin McCarthy, HT (ASCP)
Histotechnician
Tempus Labs
600 W. Chicago Ave.
Chicago IL 60654
Ph:(312) 638-6344 Ext.3835



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Re: [Histonet] Histonet Digest, Vol 158, Issue 22 Breast Specimen

[Rathborne, Toni via Histonet]

There is also a CAP checklist standard that might help you to document these 
cases, without it seeming like you're complaining. Once the offenders have it 
brought to their attention, and know that it is being documented, they may 
start to give more consideration to the sections they're putting through.

**NEW** 07/28/2015
ANP.10038 Tissue Sample Quality Phase II There is a procedure that describes 
the process by which histotechnologists provide feedback to submitting 
pathologists and pathology assistants on the quality of the gross tissue 
sections received for tissue processing.
NOTE: Inadequate fixation, overly thick tissue sections, non-decalcified bone, 
the presence of staples, etc., can lead to poor quality histologic sections 
and/or poor quality special stains/special studies.
This requirement applies to both laboratories that gross tissue and perform all 
processing onsite, as well as laboratories that gross tissue and send it to 
another laboratory for processing, embedding, and sectioning (regardless of the 
outside laboratory's accrediting organization).
Records of such feedback and corrective action taken when problems are 
identified may be incorporated into the laboratory's quality management program.
Evidence of Compliance:
✓ Records of feedback and corrective action for problems identified with tissue 
quality


Toni


-Original Message-
From: Steve McClain via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, January 30, 2017 3:14 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Histonet Digest, Vol 158, Issue 22 Breast Specimen


*** This is an External Email ***

In my experience, rushing to process fatty or inadequately fixed specimens is a 
fool's game.
In my opinion, this problem cannot be solved by the histotechs- it begins with 
the grossers and is one for the pathologists to solve at the grossing bench.

Suggestion #1 Do nothing and let the medical director pathologist/ sign it 
out/deal w this individual case.

Suggestion #2  Sometimes a decent section can be obtained after changing 
paraffin.
[place the blocks in molds and melt the blocks and change to new paraffin- let 
them sit in the embedding center in the new paraffin for 60 minutes. Re-embed 
in new paraffin (2 changes) and then re-embed.]

Suggestion #3 Reprocess these blocks if permitted, recognizing that breast 
markers (if cancerous) may be erroneous.

Suggestion #4 Seek to prevent future occurrences by adjusting behavior at the 
grossing bench.
a) first ensuring adequate fixation and b) second ensuring adequate length/time 
of processing.


Steve A. McClain, MD
631 361 4000

What is the best way to handle Breast specimens that were grossed too thick and 
did not process well?  Our medical director does not want us to reprocess the 
tissue but it is almost impossible to get even a remotely decent section. If 
anyone has any other tips please let me know as soon as possible

--

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs**

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Re: [Histonet] Lab Coats and OCT

[Rathborne, Toni via Histonet]

Heather,

We are currently using a lab coat made by White Knight, which is fluid 
resistant. I don't know if it's any cooler, but they do protect what you're 
wearing under it.

Regarding the OCT. We also get our from Sakura, and have not noticed any change 
to the consistency. Is this happening with all lot numbers, or only one? I 
would check with Sakura. There may be a recall that you're not aware of. It 
might also have something to do with temperature/storage conditions.

Toni


-Original Message-
From: Seeley, Heather via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, February 18, 2016 11:16 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Lab Coats and OCT

Hello All,





Now that the hospital is enforcing us to wear lab coats all the time, wondering 
if anyone has suggestions for some that aren't too hot, the ones we have now 
are making us sweat!



Also, we have been having issues with our OCT, we currently use the Sakura OCT 
and have use it for years. Within the last 6 months or so it has become like 
glue on the chucks even if we let it soak in hot water and scrub with a brush, 
it just doesn't want to come out! If anyone has any suggestions on brands to  
try we would appreciate it!

Thanks!


HEATHER SEELEY, HT(ASCP)
Histotechnologist
803-985-4676 OFFICE
803-327-7598 FAX

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Re: [Histonet] Temperatures

[Rathborne, Toni via Histonet]

Use a NIST certified thermometer as you would with other lab thermometers for 
comparison. 

Toni


-Original Message-
From: Walter Benton via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, February 03, 2016 11:57 AM
To: Fawn Bomar; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Temperatures

Secondary thermometer in paraffin reservoir of  embedding center.

-Original Message-
From: Fawn Bomar via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, February 03, 2016 11:23 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Temperatures

Hi All,



I was wondering how everyone tracked their cryostat and paraffin temperatures.  
Do you all just use the machine temperature, or do you all use the machine and 
a second thermometer as verification?



Thank you

Fawn
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[Histonet] OR specimens in formalin

[Rathborne, Toni via Histonet]

Good afternoon.

This question is mainly for those who attended NSH this year, but others may 
know what I'm looking for too. In the Exhibition Hall, there was a product 
which was to be used in the OR and in which the specimen was placed in a bag, 
which itself was in an instrument and was then filled with formalin depending 
on the size of the specimen. Although I didn't think that we had a need for it 
at the time, there has been some interest expressed by the OR now. If anyone 
remembers the name of this instrument or the company that sells it, I'd be 
grateful if you could respond.

Toni



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Re: [Histonet] OR specimens in formalin

[Rathborne, Toni via Histonet]

Thanks everyone for your quick responses! The product I was looking for was 
SealSAFE by Milestone.

Toni


_
From: Rathborne, Toni
Sent: Wednesday, November 11, 2015 12:01 PM
To: histonet@lists.utsouthwestern.edu
Subject: OR specimens in formalin


Good afternoon.

This question is mainly for those who attended NSH this year, but others may 
know what I'm looking for too. In the Exhibition Hall, there was a product 
which was to be used in the OR and in which the specimen was placed in a bag, 
which itself was in an instrument and was then filled with formalin depending 
on the size of the specimen. Although I didn't think that we had a need for it 
at the time, there has been some interest expressed by the OR now. If anyone 
remembers the name of this instrument or the company that sells it, I'd be 
grateful if you could respond.

Toni



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