Re: [Histonet] tissue fixation-formaldehyde concentrations which is best.
There are many books on histological technique. Most of them are expensive. The 5th edition of John Kiernan's excellent HISTOLOGICAL AND HISTOCHEMICAL METHODS will sell for $100 when it comes out in July. If that is too much for your budget, there are older books that cover 95% of what you need to know. The 4th edition of Kiernan's HISTOLOGICAL AND HISTOCHEMICAL METHODS is available used for $60, but the price may fall when the 5th edition comes out. A used 4th edition of Gretchen Humason's ANIMAL TISSUE TECHNIQUES is a great bargain at $12. Allen A. Smith Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, Florida -Original Message- From: John Kiernan [mailto:jkier...@uwo.ca] Sent: Saturday, June 06, 2015 12:31 AM To: Peter Noyce; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] tissue fixation-formaldehyde concentrations which is best. Dear Peter, Some of the information you mention as anecdotal is wrong. Formaldehyde and paraformaldehyde are well documented in original peer-reviewed papers and in all textbooks in the fields of histotechnology and histochemistry. Your anecdote about high concentrations of formaldehyde quickly form a 'shell' in the tissue and will stop good penetration and fixation to the deeper tissues has no basis in published work. Paraformaldehyde is an insoluble polymer, not non polymerized formaldehyde. There is no such thing as 4% paraformaldehyde! It is a sad fact that many labs do not contain even one book about histotechnology. Nearly all books in the field (and there are many) have plenty of references to review articles and original literature about the techniques. There are also several websites that provide links to useful papers. Check out some of the useful links on the Biological Stain Commission's site: http://biologicalstaincommission.org/useful-links/ As a graduate student, you need to work from primary sources or reliable secondary sources. When you defend your thesis, you won't want to justify your fixation or staining method by saying I got the method by asking on an internet listserver. John Kiernan Professor Emeritus Anatomy Cell Biology University of Western Ontario London,Canada = = = On 05/06/15, Peter Noyce pwno...@gmail.com wrote: Formaldehyde 37% (commonly called 100% formalin) compared to 4% ( commonly known as 10% neutral buffered formalin)-in theory the 37% should fix quicker and better BUT anecdotally it is said that high concentrations of formaldehyde quickly form a shell in the tissue and will stop good penetration and fixation to the deeper tissues AND over the years it has been said anecdotally that 4% concentration is the quickest and most complete for all sample (mammal and plant) fixation and preservation-are these true. Please do discuss the methanol or buffers that is in the formaldehyde, or discuss paraformaldehyde (which is non polymerized formaldehyde with no methanol, in water). Regards Peter Noyce PhD student. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE:Blade Rationing
I run an academic lab on a very tight budget. A paper towel used to dry washed hands is used again. Outdated dye solutions are adsorbed onto a small pile of old paper towels to save on waste disposal costs. (A quarter-pound of solid waste costs less to dispose of than 2 liters of aqueous liquid waste.) Disposable pipettes are washed and reused until the numbers wear off. I make up Vector's ImmPact SG 1.7 ml a time, store it in the fridge, and use it all week. I don't save on microtome blades.Dull blades leave holes in 4 micron sections. Sections cut with a dull blade have the annoying habit of exploding on the water bath. Dull blades tease out collagen fibers and drape them over the cells I'm trying to study. When a blade is dull it goes into the sharps box. -Allen A. Smith, Ph.D. Barry University School of Podiatric Medicine Miami Shores, FL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] picric acid paranoia
Picric acid bound to collagen is not an explosion hazard. Even if it were, the surrounding paraffin wax would cushion the picric acid to the point of making it shockproof. Most of the picric acid in a fixative ends up in the hazmat bottle rather than in the tissue. Thus even putting 50 or so blocks of tissue fixed in picric acid into a hot fire would create less blast than a hearing aid battery. Bulk picric acid, where there is no moderator between the crystals, is another story. - Allen A. Smith, Ph.D. Barry University School of Podiatric Medicine -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tyrone Genade Sent: Monday, June 03, 2013 2:30 PM To: histonet Subject: [Histonet] picric acid paranoia Hello, I am moving to the USA from sunny South Africa. I would like to bring my wax blocks with me but the fish inside them were fixed with Bouin's fluid. I'm worried the picric acid could draw the wrong sort of attention. Courier companies and US Customs (which never got back to me) haven't been able to give me an answer if they are safe to travel. The blocks have sat under my lab bench for 4 years without blowing up so I guess they are perfectly safe. Anyone have an opinion on the issues or some advice on an expert (at US customs?) to contact? I would probably ship them by surface post as it just more cost effective. Thanks Tyrone Genade PhD Department of Human Biology University of Cape Town ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Formalin
Formalin does burn. If you soak a piece of paper in formalin, you can set it on fire with a match. Formalin is also slightly carcinogenic. The biggest problem with formalin is that a few people are severely allergic to it. -Allen A. Smith, Ph.D. Barry University School of Podiatric Medicine -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, May 31, 2013 12:41 PM To: Scott, Allison D; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Formalin Formalin is THEORETICALLY (and very UNLIKELY) flammable, but putting it into a flammables cabinet is going too far. Try to find out those conflicting standards (JCAHO vs. CAP) and follow the strictest. Also please remember that many inspectors just love to show off! Some think that have to cite for something because otherwise they are not doing a good job, or do not know enough. René J. From: Scott, Allison D allison.sc...@harrishealth.org To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Friday, May 31, 2013 12:31 PM Subject: [Histonet] Formalin Hello to all in histoland. Happy Friday. Last week we were inspected by a new group of surveyors that are like JCAHO called DNV. They said that we had to much formalin stored in our gross room. We had 15 gallons. They told us that we could only have 2 gallons there at a time. I have never heard of such. We were just inspected by CAP and I might add that it was an intense inspection. They checked everything. This never came up. What is the recommended amount of formalin that can be stored in an area. Does it need to be in a flammable cabinet? The cabinet that we have the formalin in is so old that we are having a vendor to come out to check to see if it suitable for chemical storage. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-2148 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Working in a Lab alone
Normally, I work alone. Sometimes a graduate student works with me. In a few cases, where special hazards are involved (e.g. lithium aluminum hydride, diazomethane), I will work only if I am alone in my lab so that I will be the only one injured AND someone who could help is present in an adjacent lab. Allen A. Smith,Ph.D. Professor of Anatomy Barry University School of Podiatric Medicine -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Friday, May 24, 2013 10:12 AM To: pam plumlee; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Working in a Lab alone Pam I don't believe that they are any reqs regarding this, but there is a tremendous amount of information on the web associated with this topic (working alone in the lab). We have a policy here that no one can work in the lab alone, but we instituted that ourselves and we are a small business. This has been addressed at lot at universities when individuals are in the lab at all hours of the day by themselves and if something happens there is really no one else there to help. I believe I have some documents in my files that I can forward onto you if you like but I did not see any regulations specifically and policies varied from institution to institution. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 l...@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of pam plumlee Sent: Friday, May 24, 2013 7:55 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Working in a Lab alone Good morning: I'm trying to help out my CLS coworkers...they have been asked to work Saturdays-by themselves-1 person alone in the lab for approx. 4-5 hours. They are a bit worried by the security factor-although we are located in a kind of remote industrial area, the building has card key locked doors. My concern is working in the lab alone. We have many freezers, fridges and other water sources that may present a slip risk. Does anyone know if there are state, CAP, CLIA guidelines, preventative rules about this matter. We are located in California. Any help, suggestions appreciated. Pam at BioT. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Alcian Blue
Alcian blue lasts until loss of dye onto the sections weakens staining. If one runs many slides through it, it may not last 6 weeks. With light use, it may last 6 years. Allen A. Smith Professor of Anatomy Barry University School of Podiatric Medicine -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Wednesday, February 27, 2013 3:03 PM To: Rene J Buesa; Diana McCaig; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Alcian Blue Why would you use a reagent until it starts to weaken, meaning the staining intensity and results are changing over time. In my opinion that's really poor quality control. Stain intensity, specificity and sensitivity should remain constant. Liz -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, February 27, 2013 11:57 AM To: Diana McCaig; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Alcian Blue Until the staining starts to weaken René J. From: Diana McCaig dmcc...@ckha.on.ca To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Sent: Wednesday, February 27, 2013 2:55 PM Subject: [Histonet] Alcian Blue What is the shelf life of prepared 1% Alcian blue in 3% Acetic Acid? Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Osmium tetroxide staining for lipids
Osmium tetroxide solutions will fix your skin on contact, but skin grows back. Osmium tetroxide fumes will fix your corneas which are irreplaceable. Osmium tetroxide is dangerous, but it is manageable. First, make sure your fume hood draws really, really well. Put 100 ml of pH 7.2 phosphate-buffered saline into a 150 or 200 ml glass bottle. Score a 1 gram ampoule of osmium tetroxide with a file. Drop the ampoule into the bottle, close the bottle tightly, and shake it until the ampoule breaks. Let the osmium tetroxide dissolve overnight. Use as little as possible of this solution on frozen sections IN THE FUME HOOD. The glass door should be as far down as is practical. Keep your head out of the fume hood! Only your hands should be inside. Used osmium tetroxide solution and the first rinse should be saved in a tightly closed glass bottle and given to a waste hauler. Since it has salvage value, some waste haulers will take osmium tetroxide without charge. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, Florida -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, August 24, 2012 10:32 AM To: Sheila Adey; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Osmium tetroxide staining for lipids Osmium tetroxide is one of the most dangerous substances you can use in the laboratory. Your pathologist probably read some article or found an old photo of fat stained with osmium tetroxide and now wants you to do the same thing. The problem is that the fat is allowed to react to the fumes of this very nasty substance and this is a very dangerous step. Nowadays this is never done. If he wants to demonstrate fat, freeze the tissue, prepare a frozen section and us Oil Red to demonstrate fat. This is the most current measure. On the other hand, as you point out, osmium tetroxide is used in electron microscopy. René J. From: Sheila Adey sa...@hotmail.ca To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Friday, August 24, 2012 8:30 AM Subject: [Histonet] Osmium tetroxide staining for lipids Hi Everyone: One of my pathologists wants me to look into Osmium Tetroxide for staining lipids. From what I can gather on the internet, it looks like it is used in Electron microscopy for fixation and staining. Is anyone using this procedure for routine 4 micrometer sections? Thanks :) Sheila ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: disposal of formalin
We hire a licensed waste disposal company. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, Florida -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lake,Debbie Sent: Friday, August 17, 2012 11:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] disposal of formalin Would anyone be willing to share how formalin is disposed of at your facility? Neutralization, disposal off site, etc. Thank you. Debra Lake MT(ASCP) Manager Micro, Blood Bank, Pathology Marion General Hospital Marion, IN 46952 (765) 660-6521 Fax: (765-651-7330) If you are not the intended recipient(s), you are notified that any disclosure, copying, distribution or any action taken or omitted to be taken in reliance on the contents of this information is prohibited and may be unlawful. If you receive this message in error, or are not the named recipient(s), please notify the sender, delete this e-mail from your computer, and destroy any copies in any form immediately. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: counterstain for Alcian Blue (ph2.5)
The time and trouble of making up a solution of aluminum mordanted nuclear fast red repays you well. The staining solution contains to work well for an entire year. If you must have something else, brazalum, made by substituting brazilin for hematoxylin in Mayer's hemalum, works very well. The solution keeps its staining qualities for 2 or 3 months. Anatech sells brazalum ready-made as Brazilliant. Although Orth's lithium carmine gives beautiful results, I don't recommend it: it's a pain to make and keeps its staining power for only 2 weeks. Allen A. Smith Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, FL -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Wednesday, August 01, 2012 12:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] counterstain for Alcian Blue (ph2.5) Can you please let me know what options for counterstain there are other than Nuclear Fast Red or a supplier who sells the prepared solution Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Negative Reagent Control
For the last couple of years, I've thought this was true, but I didn't have the guts to say so. Thank you, Richard for bringing the truth out and getting it accepted. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, FL -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Tuesday, July 17, 2012 7:05 PM To: Histonet Subject: [Histonet] Negative Reagent Control Most of you know that I have been advocating for the elimination of the Negative Reagent Control when using a non-avidin-biotin detection system (polymer) in immunohistochemical testing. In my opinion, it is a waste of healthcare dollars and, more importantly, precious patient specimen. Last year at the NSH IHC Forum in Denver I met James Dvorak, MT(ASCP) from the College of American Pathologists. We had a long discussion about this and he agreed to support my position within CAP. With the support of James, and the help of Dr. Regan Fulton (from the CAP IHC Committee), the wording on the CAP Anatomic Pathology checklist for question ANP.22570 will be changed. The new wording includes the following sentence: Immunohistochemical tests using polymer-based detection systems (biotin-free) are sufficiently free of background reactivity to obviated the need for a negative reagent control and such controls may be omitted at the discretion of the laboratory director. I announced this change at the 2012 NSH IHC/ISH Forum this past weekend in Windsor, CT to loud applause. This one change stands to save the healthcare system millions of dollars. Thank you Jim and Dr. Fulton for making this happen! Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Recommendations for HRP anti Rabbit and mouse and AEC substrates
I have used Vector's ImmPress and ABC secondary antibody systems. I like ImmPress better: it has fewer steps, it gives me less background, and the reagents last longer. Vector's Nova Red has given me better results than any other stain that I have tried. -Allen A. Smith Barry University School of Podiatric Medicine. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra Sent: Wednesday, June 27, 2012 1:38 PM To: 'Lewis, Patrick'; 'Histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Recommendations for HRP anti Rabbit and mouse and AEC substrates We use Jackson Immunoresearch for our secondaries and the Biocare Alk. Phosph. Kit or the DAKO DAB plus kit. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick Sent: Wednesday, June 27, 2012 9:45 AM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Recommendations for HRP anti Rabbit and mouse and AEC substrates Hi everyone, I have been using DAKO Kits for HRP anti rabbit and HRP anti Mouse and AEC substrates. I love DAKO, but their kits and reagents are expensive. Can anyone offer an alternative for HRP labeled anti (Rabbit/Mouse) antibodies and AEC substrate kits. I do all my IHC manually by hand. I am doing my IHCs primarily on FFPE slides of various tissues, some of which are large enough that they take up a lot of the slide, and hence need more drops to get complete coverage. Thanks Patrick. PS: I am considering Vector Labs, and I am also looking at Biocompare to seek out more vendors. Has anyone tried Spring Bioscience's products? CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] GLASSWARE CALIBRATION
Glass glassware, including glass pipets, has always been accurate enough for my needs in making up histological stains. This is not (repeat NOT) true of pipettors with disposable tips: Some of my pipettors deliver exactly what they say they do, some deliver 50% more, and some deliver 3 times as much as their factory calibration claims. I calibrate my pipettors by weighing the average volume of water delivered in 5 trials and write the true volume on a tape label on the barrel of the pipettor. Antibody dilutions using 2 pipettors thus require a bit of calculation. Allen A. Smith Barry University School of Podiatric Medicine -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, March 28, 2012 8:39 AM To: histonet; histonet; shehnaz khan Subject: Re: [Histonet] GLASSWARE CALIBRATION Calibrating glassware is a most in analytical chemistry but is of lesser importance while preparing staining solutions that, at the end, are going to used to determine if a tissue component has been stained or not, and seldom subjected to a quantitative intensity determination. A (+) PAS-Schiff reaction is what is needed and the intensity can depend on the temperature of the reaction, the condition of the solution or the amounts of the (+) components in the tissue. With so many sources for intensity it is of little importance if your cylinder is reading exactly 100mL when you are preparing the solution or if that amount is ± 0.5mL, especially when human error has to be considered also at the moment of the preparation. You have to trust the manufacturer of your glass equipment and accept the calibration provided with each glassware as true. Mind that, in addition, those calibrations are usually made at 20ºC and I do not think that 20ºC is the room temperature in most laboratories. But, IF you want to calibrate some glassware, you will need an analytical balance (not a common piece of equipment), a good thermometer to determine the distilled water temperature, a table with the density of water at different temperatures and you have to fill your cylinder, pipette, or whatever glassware you want to calibrate to a certain mark and them weigh the water. Divide the result (in grams) between the density of the water at the temperature you made the measurement and you will get the value of the volume. Too much trouble, just trust the manufacturer of your glassware! René J. --- On Wed, 3/28/12, shehnaz khan shehnazs...@gmail.com wrote: From: shehnaz khan shehnazs...@gmail.com Subject: [Histonet] GLASSWARE CALIBRATION To: histonet histo...@pathology.swmed.edu, histonet histonet@lists.utsouthwestern.edu Date: Wednesday, March 28, 2012, 4:51 AM Hi netters, Could someone please shed some light: 1. How is calibration for glassware performed on non-Class A glassware? 2. If Class A glassware is used but no certificate is located - does it still require calibration? Thanx again. S Kahn ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Shiny side of a paraffin section
My mentor, Nick Roman, told me that sections adhere to the slide better if they go on shiny side down. Brenda Disbrey's HISTOLOGICAL LABORATORY METHODS says that laying the sections on the water bath or water droplet shiny side down makes it easier to remove creases. Benno Romeiss' MIKROSKOPISCHE TECHNIK and Manfred Gabe's TECHNIQUES HISTOLOGIQUES say that section should be mounted shiny side down without giving a reason. Most other authors do not even mention this matter. Allen A. Smith Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, Florida -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey Sent: Tuesday, February 28, 2012 6:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Shiny side of a paraffin section As all of us who cut paraffin know, the underside of each section as it comes off the blade is shiny. I've always accepted it as a fact that the shiny side always goes down on the water bath, but I've begun to wonder why. Is there a specific reason why we're all taught to put the shiny side down? What would the difference be between a 'properly' collected section and a rebelliously collected shiny-side up section? Does it even matter? Thanks! Lucie Lucie Guernsey UC San Diego lguern...@ucsd.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] topoisomerase IHC
I am having difficulty localizing topoisomerase IIalpha with IHC. Mouse primary/biotinylated horse secondary works for me only some of the time. Rabbit primary/biotinylated goat secondary doesn't work at all for me. Does anyone have any suggestions for improving the performance of either system? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Slides
Try coating the slides yourself with Vector Labs Vectabond. Neither I nor any of my graduate students have ever lost a section from a slide coated with Vectabond. -Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, Florida From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Sheila Haas [micropathl...@yahoo.com] Sent: Tuesday, August 30, 2011 7:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slides Hi all! We are having issues with the tissue falling off of our slides during routine staining. The tissue primarily lifts from the bottom sections on the slides. We have had our stainer checked and have checked ourselves, the oven appears to be working properly and the water pressure is fine. I tried two different slide manufacturers, both silane coated, and are getting the same result. Not sure where to go at this point except to try anon-coated slide. Any suggestions on a manufacturer or vendor?? Thanks in advance. Your help is always appreciated! Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Formalin Fixation.
Formalin fixation kills most but not all bacteria. A significant percentage (~10%) of the mycobacteria present in the tissue remain viable. Tuberculosis bacteria have been cultured from ffpe blocks. Prions (Creutzfelt-Jacob disease) are believed to survive formalin fixation. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Podiatric Medicine -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of smitha rayadurg Sent: Wednesday, June 22, 2011 4:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin Fixation. Dear all Does Formalin fixation of tissues kill bacteria viruses if present in the tissue? Would you please email any references on the effect of Formalin fixation on the stability of microbes (Viruses, Bacteria Parasites etc) in the tissues? Grateful Thanks Smitha. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Formalin Fixation.
The reference is K.F. Gerston et al: Viability of mycobacteria in formalin-fixed tissues. International Journal of Tuberculosis and Lung Diseases. 2:521-523 (1998). -Allen A. Smith, Ph.D. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of smitha rayadurg Sent: Wednesday, June 22, 2011 4:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin Fixation. Dear all Does Formalin fixation of tissues kill bacteria viruses if present in the tissue? Would you please email any references on the effect of Formalin fixation on the stability of microbes (Viruses, Bacteria Parasites etc) in the tissues? Grateful Thanks Smitha. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: block disposal
The best solution would be to give them to the Cooperative Human Tissue Network. Allen A. Smith Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, Florida -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fleming, Jackie M Sent: Monday, June 06, 2011 12:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] block disposal How do facilities dispose of large amounts of paraffin blocks after the after the CAP 10 year guidelines? Jackie Fleming HT ASCP Allina Medical Laboratory Histology Technical Consultant phone:612-863-4773 Internal Zip 11136 This message contains information that is confidential and may be privileged. Unless you are the addressee (or authorized to receive for the addressee), you may not use, copy or disclose to anyone the message or any information contained in the message. If you have received the message in error, please advise the sender by reply e-mail and delete the message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Counter top immunos
I like the Vector ABC horseradish peroxidase kits, especially with the Nova Red chromogen. Mouse primary antibodies usually give me better results than rabbit or goat primaries. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marshall, Kimberly K Sent: Thursday, June 02, 2011 9:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Counter top immunos Hello Histo folks Hope everyone is having a great weekI am writing to get some info on immuno kits for staining just by hand, like back when I first started in this field. We are a small lab in Alaska and all of our immunos are sent out of state, We are looking to do some of them here and try to save money, but don't really need a stainer. So are there any kits that are better than others? Is there anyone that does immunos this way that would give some much needed advise??? Again thanks in advance for all the imput... Kimberly ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Neutralizing Formalin
We bought materials for neutralizing formalin and started to use them. Two years later, the local EPA authorities told us to stop because we lacked the competence to neutralize hazardous materials. Our neutralizing solution became a hazardous material. We now pay a waste hauler to take our formalin. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Evans, Andria B Sent: Thursday, April 21, 2011 12:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Neutralizing Formalin Is anyone out there in Histoland Neutralizing their formalin before discarding it down the drain? We are looking into doing this because of our water authorities and we are finding that it is very costly. Has anyone found a cost efficient way to do this? I would need to know what supplier you are purchasing it from and the name of the product. Thank you in advance!! Andria B Evans HTL(ASCP)CM Lancaster General Hospital 555 North Duke Street Lancaster, PA 17604 (717)544-5511 ext: 77329 This email was sent securely from the LGHealth Email Service Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: microtome safety
My mentor used forceps. Thus I have always used forceps. I think the paraffin ribbon would melt onto fingers on a warm day. Allen A. Smith Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, Florida -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Tuesday, March 08, 2011 8:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] microtome safety Morning all! I need some quick responses to this question: do you use your fingers or an instrument of some sort to pull your paraffin ribbons off the block when sectioning? For those that do not use their fingers, what do you use? If forceps, are these the typical lab forceps or a special type? Thanks so much! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartl...@cdc.hhs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] re: storing acetic acid
We have separate storage cupboards for inorganic acids (many of which are oxidizing agents) and organic acids (many of which are flammable). If we didn't have an organic acids cupboard, we would store it with organic solvents. The reaction of acetic acid with nitric acid might be violent, the reaction of acetic acid with ethanol is slow and quiet. -Allen A. Smith Professor of Anatomy Barry University School of Podiatric Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] shrinkage
When I wrote my thesis on sweat glands, my experience was that fixation in Helly's fluid (Zenker-formol) and paraffin embedding caused serious shrinkage: about 10-15% in each dimension. Allen A. Smith Professor of Anatomy Barry University School of Podiatric Medicine -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Tuesday, August 24, 2010 11:50 AM To: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] shrinkage I think tissue shrinkage is a fairy tail, told by pathologists at the grossing. ;) Sure, it is a little bit large, but it will shrink.. Too big blocks stay too big while embedding. **just for smiling** There's a study by Cecil Fox 1985 (Formaldehyde fixation), who showed that shrinkage while fixation occurs only with concentrated formaldehyd, perhaps due to the methanol-part in commercial formalin. For dehydration, de-fattation and infiltration I think, it depends on the gradationsteps of the solutions, the applied temperature and the ingredients of the tissue. With our standard protocol (13 hours VIP) I never found excessiv shrinkage. So, like always, it depends... Gudrun Lang -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Tench, Bill Gesendet: Dienstag, 24. August 2010 17:33 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] shrinkage I have never seen a study, but when training, we were taught that fixation and processing could result in up to 5-10% shrinkage. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations - Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Disposal of MMA BPO Waste
This is what comes of letting people with little or no training in chemistry call themselves chemical waste mangers. Yes, methyl methacrylate is highly reactive when mixed with 1% benzoyl peroxide: it forms a hard, brittle polymer. Pour your left over methyl methacrylate and benzoyl peroxide into a disposable weighing boat and heat it overnight at 60 C and over another night at 80 C. The resultant polymer is commercially sold as Plexiglass; it can be safely disposed of as ordinary trash. A much higher concentration of benzoyl peroxide (10% or more) could explode when heated. Chemical potential depends on concentration. -Allen A. Smith, M.A. (chemistry), Ph.D. (anatomy) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Brad Herschler Sent: Sunday, June 20, 2010 9:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Disposal of MMA BPO Waste We use an MMA embedding technique similar to that used for histology in our lab. We sent a request to our campus Chemical Waste Management for the MMA waste mixture to be disposed of, but they are refusing because they say the mixture is too hazardous. The mixture contains 1gram of benzoyl peroxide (BPO) per 100mL of MMA. Chemical Waste Management informed us that MMA in the presence of BPO is highly reactive. We would like to know: for histology, how is MMA + BPO waste normally disposed of? Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: proper disposal of chemicals
Look up Waste Hauling in the Yellow Pages. Ask every company listed for a quote. Make sure the ammoniacal silver nitrate is wet; when it dries, it can rearrange to silver azide, a powerful and touchy primary explosive. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hutton, Allison Sent: Friday, May 07, 2010 10:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] proper disposal of chemicals I have a fair amount of different chemicals that are expired and need to be disposed of. I am looking for advice on how to properly dispose of the following chemicals beyond in accordance with local, state, and federal guidelines: *Gold Chloride 0.2% aq *Schiff reagent *Aniline Carbol Fuchsin *Gomori Trichrome Stain *Glycerol *Iodine Powder *Ammoniacal Silver Nitrate *1N Sodium Hydroxide Any advice would be greatly appreciated I am located in PA Thanks, Allison ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Mucicarmine Stains
I use Sheehan and Hrapchak's formula and stain 1/2 hour at 45 degrees C. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Evans, Andria B Sent: Monday, January 25, 2010 2:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mucicarmine Stains We are having some issues with our Muci stain being pale. I would like to know what everyone in Histoland is using for the Muci Stain. Thanks! Andria B Evans, HTL(ASCP)CM Anatomic Pathology York Hospital 1001 S. George Street York, PA 17405 You can learn a lot more from listening than you can from talking. Find someone with whom you don't agree in the slightest and ask them to explain themselves at length. Then take a seat, shut your mouth, and don't argue back. It's physically impossible to listen with your mouth open. -John Moe Maturity is accepting imperfections. CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . __ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] sharpen permanent knife blades
C.L. Sturkey (www.sturkey.com) in Lebanon, Pennsylvania, sharpens permanent microtome knives. They also have two grades of disposable blades. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Podiatric Medicine -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of MARCELLYN STONE Sent: Tuesday, December 22, 2009 9:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] sharpen permanent knife blades Please help:-). I am looking for information on anyone who sharpens permanent knife blades. Thanks Marcy CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for use by the designated recipients named above. They are intended solely for these recipients. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Calvert Memorial Hospital immediately by telephone at (410) 535-8282 and destroy all copies of this communication and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [histonet] Cleaning and re-using slides
Cleaning slides strikes me as a very poor use of student time. If they are paid even 3/4 minimum wage, it is uneconomical. If they are unpaid, it is unfair to set them a task they can learn nothing from. -Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Podiatric Medicine Miami shores, FL -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nicholas Evans Sent: Monday, November 30, 2009 1:28 PM To: histonet@lists.utsouthwestern.edu Subject: [histonet] Cleaning and re-using slides Dear all, In our lab our boss is adamant that we must clean and recycle old unmounted microscope slides (which have paraffin and cryo sections on them). It is driving us nuts, as the process of cleaning the slides is incredibly tedious and labor-intensive - it is vital that the slides are spotlessly clean before re-treating. We (when I say we, i mean the student lackies who get drafted in the lab, not me, hoho) currently physically scrub them with various solvents, such as citrisolve, acetone etc. we use Fisherbrand Superfrost/plus slides. Anyone got any ideas how to speed this up/automate - is there a machine available? And would anyone care to vote on the lunacy or soundness of this idea? (Please post to me, not the list.) We are not a clinical lab, by the way. Nick ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: discarding old dry chemicals with no expiration date
The stuff found in the Judean caves is kal ilan, indigo derived from Indigofera tinctoria. It is chemically identical to tekhelet, indigo derived from Murex trunculus by photochemical debromination of Tyrian purple. Although some second and third century Jews, knowingly or unknowingly, used kal ilan instead of tekhelet, Rabbinic opinion has generally held that only tekhelet is kosher for dyeing the fringes. However, the Talmud also remarks that, Only God can tell the difference between tekhelet and kal ilan. Modern spectrometry can detect traces of cellulose in kal ilan, which are not present in tekhelet. Allen A. Smith Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, Florida -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Thursday, October 01, 2009 10:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: discarding old dry chemicals with no expiration date Kelly D. Boyd, BS, HTL (ASCP) at Harris Histology Services in Greenville NC describes having a CLIA inspector ordering that all dry chemicals older than 10 years be discarded. Allen Smith sums it up The problem is letting people with little or no knowledge of chemistry make chemical decisions. To which I would add: and a system in which inspectors from agencies accountable to no one (e.g. JCAHO) are allowed to require summary changes in laboratory procedures, with no appeal allowed. Thirty years ago most histology labs did almost all of their own preparations - today making a 1% solution of periodic acid is considered high-level manufacturing. Allen Smith goes on to observe that Indigo used by Bar Kochba's soldiers to dye the fringes of their prayer shawls in the second century has been found in caves in the Judean desert; it is still indigo. Was this indigo, or was it the indigo-like dyes obtained from shellfish that they used? This remains a lively subject of debate among Jewish scholars. Present day Jews do not dye the fringes (tzitzit) of their prayer shawls (tallit or tallis), thinking that the technique has been lost (a vociferous fringe group - you might say - is using shellfish-derived dyes and has a cool T-shirt - I want one). Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] discarding old dry chemicals with no expiration date
The problem is letting people with little or no knowledge of chemistry make chemical decisions. Some chemicals are unstable on the shelf, some are not. Methyl green decomposes in a year or two, tetracycline decomposes in 5 years, indigo lasts for millennia. Indigo used by Bar Kochba's soldiers to dye the fringes of their prayer shawls in the second century has been found in caves in the Judean desert; it is still indigo. Allen A. Smith Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, Florida -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jstaruk Sent: Wednesday, September 30, 2009 4:40 PM To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] discarding old dry chemicals with no expiration date I always have a problem determining the expiration date of sodium chloride! Jim ___ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, September 30, 2009 4:27 PM To: histonet@lists.utsouthwestern.edu; Kelly Boyd Subject: Re: [Histonet] discarding old dry chemicals with no expiration date I was going to comment about how important some people seem to feel to find something to add to the inspection report even when it is unsubstantiated, about how many of those chemicals are extracted from mines where they have existed for eons, how the only important thing is to make sure that those designated as anhydrous have to be kept that way in order to assure the quality of the solutions and that everything else is almost ridiculous, but I better don't because I may hurt some feelings! René J. --- On Wed, 9/30/09, Kelly Boyd kdboydhi...@yahoo.com wrote: From: Kelly Boyd kdboydhi...@yahoo.com Subject: [Histonet] discarding old dry chemicals with no expiration date To: histonet@lists.utsouthwestern.edu Date: Wednesday, September 30, 2009, 1:24 PM Our lab recently had our CLIA inspection and the surveyor informed me that we need to discard any dry chemicals with an opened date of more than 10 years, even though none of the dry chemicals have an expiration date. This was not written up as a deficiency, but it was suggested we follow up on this for our next re-certification. I am sure it is best to keep the chemicals current, but is this what all labs are doing? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com Tele (252)-830-6866 (800)-284-0672 Cell (252)-943-9527 Fax (252)-830-0032 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] formalin storage
Does he require his wife to wear chain mail gloves while paring potatoes? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Friday, September 11, 2009 9:31 AM To: Jean Warren Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu; Riesen, Rebecca Subject: Re: [Histonet] formalin storage I once had my safety officer insist I wear chain maille gloves while cutting frozen sections. They didn' t care about all the reasons I gave them why I shouldn't - like it would be impossible to use the machine while wearing them, and the patient would have to lie on the operating table longer waiting to find out if their entire colon was going to be removed. Jean Warren jwarre...@cinci.rr.com Sent by: histonet-boun...@lists.utsouthwestern.edu 09/11/2009 08:25 AM To Riesen, Rebecca rebecca.rie...@nchmd.org, histonet@lists.utsouthwestern.edu cc Subject Re: [Histonet] formalin storage No, it is ridiculous. Safety people tried to argue this with us years ago. One of our pathologists told them, How can something that is almost 90% water be a combustion hazard? - Original Message - From: Riesen, Rebecca rebecca.rie...@nchmd.org To: histonet@lists.utsouthwestern.edu Sent: Friday, September 11, 2009 9:15 AM Subject: [Histonet] formalin storage We have been directed by our Safety Officer to store all formalin (37% and 10% NBF) in a flammable storage room, cabinet or container. Yes, 37% Formalin we do store in this manner, but I have never heard of this requirement for 10%NBF. I looked on line to many MSDS sheets from different vendors and found only one that stated such storage requirements for 10% NBF. During this search I found all but one company states that formalin is not flammable. I brought this to the Safety Officer. He agrees that it is not flammable but that it IS combustible. Combustible=Flash point of 100F to 200F. Of the dozen sites I visited I found the following data concerning the Flash Point of 10% NBF: from NA / 200F / 122F to 185F. The NFPA (National Fire Protection Agency) guideline of no more than 1 gallon in a flammable storage container and 1 gallon outside of a safety cabinet/container per 100 square feet is already quite limiting. Using this guideline, we have calculated acceptable volumes of the known flammables (Alcohols and Xylenes) we can store. Adding 10% NBF to the equation will have us traveling to our bulk storage area constantly. Does anyone out there store 10%NBF in flammable cans/cabinets? Riesen, Rebecca rebecca.rie...@nchmd.org NCH Healthcare Systems Direct 239-436-5000 x2188 Fax 239-436-6767 Visit our website at http://www.nchmd.org CONFIDENTIALITY NOTICE This email and any files transmitted with it are from the NCH Healthcare System. This message is confidential and is intended only for the addressee. If you are not the intended recipient or have received this email in error, please call us immediately at (239) 436-5000 and ask to speak to the message sender or promptly email the message sender of the delivery error and then delete the message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] formalin storage
Formaldehyde is flammable; formalin is not. Above 122 degrees Fahrenheit enough formaldehyde evaporates from formalin to create a modest fire hazard in the fumes just above the liquid. Try this: pour 3 ml of formalin (37% formaldehyde) into a watch glass under a fume hood (fan off). Touch a match to it. The match will flare briefly in the fumes and that is all (unless the temperature is above 122 F). -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jean Warren Sent: Friday, September 11, 2009 9:25 AM To: Riesen, Rebecca; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] formalin storage No, it is ridiculous. Safety people tried to argue this with us years ago. One of our pathologists told them, How can something that is almost 90% water be a combustion hazard? - Original Message - From: Riesen, Rebecca rebecca.rie...@nchmd.org To: histonet@lists.utsouthwestern.edu Sent: Friday, September 11, 2009 9:15 AM Subject: [Histonet] formalin storage We have been directed by our Safety Officer to store all formalin (37% and 10% NBF) in a flammable storage room, cabinet or container. Yes, 37% Formalin we do store in this manner, but I have never heard of this requirement for 10%NBF. I looked on line to many MSDS sheets from different vendors and found only one that stated such storage requirements for 10% NBF. During this search I found all but one company states that formalin is not flammable. I brought this to the Safety Officer. He agrees that it is not flammable but that it IS combustible. Combustible=Flash point of 100F to 200F. Of the dozen sites I visited I found the following data concerning the Flash Point of 10% NBF: from NA / 200F / 122F to 185F. The NFPA (National Fire Protection Agency) guideline of no more than 1 gallon in a flammable storage container and 1 gallon outside of a safety cabinet/container per 100 square feet is already quite limiting. Using this guideline, we have calculated acceptable volumes of the known flammables (Alcohols and Xylenes) we can store. Adding 10% NBF to the equation will have us traveling to our bulk storage area constantly. Does anyone out there store 10%NBF in flammable cans/cabinets? Riesen, Rebecca rebecca.rie...@nchmd.org NCH Healthcare Systems Direct 239-436-5000 x2188 Fax 239-436-6767 Visit our website at http://www.nchmd.org CONFIDENTIALITY NOTICE This email and any files transmitted with it are from the NCH Healthcare System. This message is confidential and is intended only for the addressee. If you are not the intended recipient or have received this email in error, please call us immediately at (239) 436-5000 and ask to speak to the message sender or promptly email the message sender of the delivery error and then delete the message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Start Up Lab
I can't imagine why any one would use dioxin, which used to be used as an insulator and heat convector in electron microscope transformers, as a clearing agent. I think you mean dioxane. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, July 23, 2009 12:13 PM To: Edwards, R.E.; Montina Van Meter; Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Hah! Sure do. -Original Message- From: Edwards, R.E. [mailto:r...@leicester.ac.uk] Sent: Thursday, July 23, 2009 11:51 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Montina Van Meter; Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab You still have fingers!?. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 23 July 2009 16:44 To: Montina Van Meter; Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab And I used to use dioxin in my tissue processor and would actually dip gauze in it to clean paraffin off my fingers. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Montina Van Meter Sent: Thursday, July 23, 2009 11:31 AM To: Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on cigarettes with an ashtray perched on top of her microtome. When we had inspections everyone would put their contraband in their personal drawer. I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood. That supervisor also smoked in the lab! I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay. Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it! Too funny! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of theci...@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -Original Message- From: Lynette Pavelich lpave...@hurleymc.com Date: Wed, 22 Jul 2009 18:07:07 To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu; cing...@uwhealth.org Subject: RE: [Histonet] Start Up Lab Gosh.I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh.. Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire cing...@uwhealth.org wrote: In the lab?!? For shame. :) From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Wed 7/22/2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Start Up Lab And a coffee pot. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu
RE: [Histonet] histology for kids
If you ripen it with air or sodium iodate, alum hematoxylin is quite safe. FDC green #3 is food grade fast green FCF, an excellent stain for collagen. FDC yellow #5 is tartrazine, a plasma stain. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Wednesday, July 22, 2009 9:58 AM To: Histonet Subject: [Histonet] histology for kids Hello All, My company is hosting an in-house science awareness day for local grade-school students. I would love to teach them about histology, but all of the demonstrations need to be done in our conference room (thus, nothing hazardous). Does anyone know of any house-hold dyes (grape juice, food coloring, beet juice, etc) that would stain tissue elements on slides? I would like to bring down some deparaffinized tissues and stain them with something and throw a coverslip on (water-mounted) so that they can look at the tissue with a microscope. I will also bring some already prepared slides (wtih real stains) for them to look at. Any ideas? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] hood for manual special stains
I think a fume hood and a safety shower should be standard laboratory fixtures. I have a fume hood. I use it for manual coverslipping. I require my students to do the same. OSHA says that exposure to xylene fumes can cause liver damage. There is also a persistent rumor that repeated exposure to xylene fumes can cause Reynaud's disease. I handle formalin (37% formaldehyde) in the fume hood, and I would insist on having the hood if I handled full-strength formalin very often. I handle trifluoroacetic acid in the fume hood; I wouldn't use it at all without the hood. I wouldn't use ammonium sulfide without a fume hood. No one should ever handle osmium tetroxide outside of a fume hood. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, Florida -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Love Sent: Wednesday, June 24, 2009 6:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] hood for manual special stains The hospital where I work just opened a new lab. The histology department is now combined with cytology and is made up of me, a second histotech, a cytology prep tech, and our cytotech supervisor. We didn't have a hood in the old lab, so we did our manual special stains on a workbench with weak, overhanging vents that looked like lamps. The one hood in our new department completely belongs to the cytology prep tech. She has her centrifuge and a lot of other equipment in it, and my supervisor does not want me or the other histotech to share it. Some of our workbenches have small vents built into the walls, but when I do my specials there, I still smell chemicals. Do most histotechs today use hoods to do manual special stains or am I being too wishful? _ Hotmail(r) has ever-growing storage! Don't worry about storage limits. http://windowslive.com/Tutorial/Hotmail/Storage?ocid=TXT_TAGLM_WL_HM_Tutorial_Storage_062009___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Gross photography/macrophotography
I have been very happy with the Olympus C-7000. It will focus at 3 1/4 inches (8 cm) in its macro mode. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Reuel Cornelia Sent: Wednesday, May 20, 2009 4:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gross photography/macrophotography Hello histonetters, I am looking for a good digital camera for gross photography. Any recommendations that works with your lab will be beneficial. Thank you. Happy memorial day!!! Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Question about DAB waste
We keep our DAB waste in a plastic container and give it to our waste disposal company every 6 months. No one has objected to this procedure. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, FL -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer Campbell Sent: Wednesday, May 13, 2009 8:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question about DAB waste We just purchased a Dako Autostainer and are in the process of figuring out how to get rid of the haz waste that we will be generating. The machine separates the haz from non-haz, thankfully. I've been communicating with our waste facility and have been having some difficulty. They want to know a lot of specifics like, how much waste we will be brining to them at a time and how often, what sort of containers we will be bringing it in etc. Do you let the haz waste carboy get to a certain level before emptying it? Is it ok to pour this waste into plastic containers of some sort (we were going to use empty distilled water bottles) to transport them to the facility? I would appreciate any insight. Thank you. Jennifer C. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Question about DAB waste
We were told that, in Miami-Dade County, we would have to apply for a license to do this. We gave up on the cumbersome licensing procedure. Allen A. Smith, Ph.D. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Wednesday, May 13, 2009 12:10 PM To: histonet@lists.utsouthwestern.edu; jcampb...@vdxpathology.com Subject: Re: [Histonet] Question about DAB waste We detoxify our DAB using this method from: HAZARDOUS MATERIALS IN THE HISTOPATHOLOGY LABORATORY, by Dapson Dapson, fourth edition, pg 184. 1. Prepare the following aqeous stock solutions: a. 0.2M potassium permanganate (3.16% or 31.6g KMnO4/liter) b. 2.0M sulfuric acid (11.2% or 112 mL concentrated acid/liter) 2. Dilute DAB solution of necessary so that its concentration does not exceed 0.9 mg/ml. 3. For each 10ml of DAB solution, add: a. 5ml 0.2 M potassium permanganate (3.16% or 31.6 g/liter) b. 5ml 2.0 M sulfuric acid (196 g/liter, 107 ml/liter or 10.7%) 4. Allow mixture to stand for at least 10 hours. It is now non-mutagenic. 5. Decolorize the mixture with ascorbic acid (add powder until color disappears). This too is an oxidation/reduction reaction. 6. Neutralize the decolorized mixture with sodium bicarbonate (test with pH meter, pH paper or dipsticks). Note: we recommend using magnesium hydroxide/oxide instead of sodium bicarbonate. 7. Discard the solution down the drain provided that local wastewater authorities have given their approval. Hope this helps, Lynette Jennifer Campbell jcampb...@vdxpathology.com 05/13/09 8:51 AM We just purchased a Dako Autostainer and are in the process of figuring out how to get rid of the haz waste that we will be generating. The machine separates the haz from non-haz, thankfully. I've been communicating with our waste facility and have been having some difficulty. They want to know a lot of specifics like, how much waste we will be brining to them at a time and how often, what sort of containers we will be bringing it in etc. Do you let the haz waste carboy get to a certain level before emptying it? Is it ok to pour this waste into plastic containers of some sort (we were going to use empty distilled water bottles) to transport them to the facility? I would appreciate any insight. Thank you. Jennifer C. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: I'm outta here
I can spot spam and o.t. posts and delete them in a half second each. The questions on Histonet make me aware of the extent of use of special stains, and the potential for new stains. Jjob postings are forwarded to the career service office here. The safety warnings are often very useful. HIstonet is a great source of information on antigen retrieval techniques and antibodies. I would really miss Histonet. --Allen A Smith, Ph.D. Barry University School of Podiatric Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Current books for Histotechnology
-Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Friday, March 20, 2009 4:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Current books for Histotechnology Our library has funds available to purchase books for the Histotechnology program. The problem is that we need current books. We have the latest Bancroft and Gamble. Any other suggestions for books that are newer than 2000? I have suggested John Kiernan's latest. By the way I did find a copy of Sheehan for $2,400!! Jennifer MacDonald Education Coordinator, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdon...@mtsac.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Current books for Histotechnology
Definitely get the 4th edition of Kiernan. Also get the latest edition of Polak and van Noorden's INTRODUCTION TO IMMUNOCYTOCHEMISTRY. If you don't already have them, used second or third editions of Lillie's HISTOPATHOLOGIC TECHNIC AND PRACTICAL HISTOCHEMISTRY and Pierce's HISTOCHEMISTRY, THEORETICAL AND APPLIED are surprisingly useful. -Allen A. Smith,Ph.D. Barry University School of Podiatric Medicine Miami Shores, Florida -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Friday, March 20, 2009 4:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Current books for Histotechnology Our library has funds available to purchase books for the Histotechnology program. The problem is that we need current books. We have the latest Bancroft and Gamble. Any other suggestions for books that are newer than 2000? I have suggested John Kiernan's latest. By the way I did find a copy of Sheehan for $2,400!! Jennifer MacDonald Education Coordinator, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdon...@mtsac.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Current books for Histotechnology
For an atlas, Ross and Pawlina's HISTOLOGY, Wheater's FUNCTIONAL HISTOLOGY, or Gartner and Hiatt's COLOR ATLAS OF HISTOLOGY are all good. They are also more accurate than DiFiore. -Allen a. Smith,Ph.D. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Monday, March 23, 2009 11:55 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Current books for Histotechnology I would strongly recommend di Fiore's Atlas of Histology by Victor P. Eroschenko. I don't know what edition its in now. I used it when I went through my program. It is great for microscopic anatomy, especially when combined with actual slide viewing. Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Jennifer MacDonald Sent: Fri 3/20/2009 3:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Current books for Histotechnology Our library has funds available to purchase books for the Histotechnology program. The problem is that we need current books. We have the latest Bancroft and Gamble. Any other suggestions for books that are newer than 2000? I have suggested John Kiernan's latest. By the way I did find a copy of Sheehan for $2,400!! Jennifer MacDonald Education Coordinator, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdon...@mtsac.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Disposal of Formaldehyde
Formaldehyde can mess up the bacteria that treat the sewage. We used to neutralize our formaldehyde with Richard Allen's Vytac and send the results down the drain. Two years ago we were told that we would have to get a license and do quantitative analysis on each batch in order to continue using Vytac. Now we pay a waste hauler to dispose of our annual gallon of formalin. If we had a lot of it, we would recycle it ourselves. Allen A. Smith, Ph.D. Barry University School of Podiatric Medicine Miami Shores, Florida -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jessica Piche Sent: Friday, March 13, 2009 10:11 AM To: histonet Subject: [Histonet] Disposal of Formaldehyde Hi All, We have a question regarding the disposal of formaldehyde. We were told at our hospital that a consultant said it was okay to dump formaldehyde down the drain. I believe they said it was okay to dump 15 gallons or so a day! We are not to fond of this idea and would like to know what everyone else is doing. How is everyone disposing of their formaldehyde? We would be especially interested in what other hospitals in CT are doing. Thanks, Jessica Piche-Grocki, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] query regarding IHC slide
A lot depends on the stain chosen. I have used Vector's Nova Red for the last 3 years. I have seen no fading of my older Nova Red slides. Prof. Allen A. Smith Barry University School of Podiatric Medicine Miami Shores, Florida -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of shazana hilda Sent: Monday, February 02, 2009 9:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] query regarding IHC slide Dear All, Is anyone has experience with faded IHC-slide stained? I've done my IHC stained on few markers and after 3-4 months I viewed the slide and it seems that the stain became faded.Does anyone has opinion regarding this matter and how to troubleshoot it? Any response are greatly appreciated. Regards. Shazana Hilda ShamsuddinUniversiti Sains Malaysia, Off. no: +609- 766 4440 Fax no: +609- 765 3370 Email: hilda_1...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] silver stain for mouse intestinal nerves
Almost any method will work in intestine. I have had good results with Holmes' stain and more impressive results with Kiernan's method. I have also had good results with Winkelmann's method, but it is terribly time consuming. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Helen E Johnson Sent: Monday, January 12, 2009 10:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] silver stain for mouse intestinal nerves I am looking for a silver stain method that would stain nerves in intestines of mice. Helen Johnson (he...@health.state.ny.us) IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: HE help
Some tap waters blue hematoxylin very well, some not so well. I avoid the issue of differences in tap waters by blueing in 1.2% aqueous lithium carbonate (almost a saturated solution). - Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, FL -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Charles, Roger Sent: Friday, January 09, 2009 9:14 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] HE help Hello All, TGIF I'm trying to help out one of my veterinarian pathologist in getting some information on how to increase the bluing of the HE staining. Presently we are using pre made Mayers Hematoxylin from Sigma with an automated schedule as follows: 1. Drying 45min 2. citrisolve 3 min 3. citrisolve 30 sec 4. citrisolve 3min 5. 100% 45 sec 6. 100% 45 sec 7. 100% 3 min 8. 95% 30 sec 9. 95% 1 min 30 sec 10. Tap water 1 min 11. Hematoxylin 8 min 12. tap water 4 min 13. eosin 1 min 30 sec 14. 95% 1 min 15. 95% 1 min 16. 95% 1 min 17. 100%1 min 18. 100%1 min 19. 100%1 min 20. Citrisolve 1 min 21. Citrisolve 1 min 22. Citrisolve 1 min 23. Citrisolve up to 120 min The pathologist is stating the slides are too eosinic and would like greater bluing. My question is are there limitations to the Mayers Hematoxylin on how blue it will actually get and is there something else we can change in our schedule to increase the bluing or decrease the eosin to get the desired affect? Thanks to all Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Alias identity
While I prefer to go by my own name, I recognize that some people rightly fear to do so. An anonymous source may still have something worthwhile to say. Experience does not always teach well. The wise and experienced Linus Pauling and Erwin Chargaff were wrong about the structure of DNA; the brash young James Watson was right. -Allen A. Smith Professor of Anatomy School of Podiatric Medicine Barry University Miami Shores, FL -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of John Kiernan Sent: Monday, December 08, 2008 3:18 PM To: Amber McKenzie Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Alias identity Amber McKenzie [EMAIL PROTECTED] wrote: . . .We are all supposed to be professionals asking each other for advice/suggestions on the Histonet - who cares who each person is? If I post a question, I don't care if it's Jane Doe answering or John Smith ... That's true, and it certainly makes no difference if the person asking a question hides behind an alias. But what about the person answering? Can you act upon advice from an unknown source? Jane Doe might be experienced and wise, whereas John Smith might be some pimply youth who only thinks he knows all the answers. John Kiernan Anatomy, UWO London, Canada = = = ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] B5 fixative
B5, Schaudinn's, SUSA, Helly's, and Zenker's fixatives contain mercury (II) ions, which are VERY poisonous. Sewage bacteria convert mercury (II) to methyl mercury, which is very, very poisonous. (For a shocking example of mercury toxicity, see the photo essay MINAMATA by W. Eugene Smith.) When these fixatives are used, the fixative and the first washing must be given to a waste disposal company that can recycle it. B5 substitutes substitute a less poisonous ion, usually zinc (II), for mercury. These substitutes contain other things that are more poisonous than zinc. I store them as if they were purely the most poisonous component and give them to a waste disposal company twice a year. -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Bryan Watson Sent: Monday, October 20, 2008 10:04 AM To: Piero Nelva; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] B5 fixative For all of the listed B5 substitutes mentioned, how are they disposed of and are they significantly safer? Piero Nelva [EMAIL PROTECTED] 10/17/2008 17:59 I agree. The Occ Health and SAfety representative is your best bet to consign the B5 to history. Piero Nelva Anatomical pathology Monash Medical Centre Australia - Original Message - From: Richard Cartun [EMAIL PROTECTED] To: histonet@lists.utsouthwestern.edu; Bryan Watson [EMAIL PROTECTED] Sent: Saturday, October 18, 2008 12:46 AM Subject: Re: [Histonet] B5 fixative If you are having a hard time with a pathologist regarding the use of B5, get your safety people involved. They will take care of it. We stopped using B5 a very long time ago. We use formalin for all our lymph nodes and bone marrows. No one should be using B5 and manufacturers should stop making it. Richard Richard W. Cartun, Ph.D. Director, Histology Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Bryan Watson [EMAIL PROTECTED] 10/17/08 9:21 AM How many still use B5 fixative? And for those who do not, what do you use as a replacement? One of our pathologists is insistent on using B5, and we'd rather try to get away from it. Thanks, Bryan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.173 / Virus Database: 270.8.1/1731 - Release Date: 10/17/2008 7:01 PM ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
