Re: [Histonet] Number of blocks
No problem. This will list all articles by Buesa RJ http://www.ncbi.nlm.nih.gov/pubmed?term=Buesa%20RJ%20[au] Greetings, V. Neubert Am 25.10.2012 17:26, schrieb Rene J Buesa: I do not know how to do that! René J. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Does xylene cause skin cancer?
Debbie: Are the 30 min official? Any standard procedure that states 30 min as maximum? If you can get a hold of them, try using Nitrile gloves as these have a higher chemical resistance than latex. I use them and change every 30 minutes to avoid breakthrough. Debbie Faichney Institute of Aquaculture University of Stirling UK -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 22 February 2012 13:09 To: histonet@lists.utsouthwestern.edu; Jenny Vega Subject: Re: [Histonet] Does xylene cause skin cancer? There is no evidence in the literature about skin cancer produced by xylene, although dermatitis are well documented. Regardless you should use gloves whenever your hands can get in contact with any chemical as a good safety practice. If your colleagues do not want to use gloves, that is their prerogative, as is yours to wear them. René J. --- On Tue, 2/21/12, Jenny Vegahistotech...@gmail.com wrote: From: Jenny Vegahistotech...@gmail.com Subject: [Histonet] Does xylene cause skin cancer? To: histonet@lists.utsouthwestern.edu Date: Tuesday, February 21, 2012, 10:17 PM I am asking this because in my job we mount slides by hand, and my coworkers don't like to use gloves because it leaves a residue of latex in the back of the slides. I really don't feel comfortable mounting without gloves because I heard that xyelene can cause cancer. Some people I know personally has told me that this is not possible, but I read in some places that xylene could a possible carcinogen. I have already gotten contact with xylene in my hands a couple of times and I am worried. Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] picric acid
Just wipe away any drops after closing the bottle. But your solution makes nice shiny plastic objects become ugly dull yellow plastic objects. Avoid spilling ;) I am curious how big an explosion there would be from 1% picric acid in acetone if a little dried around the cap. Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] adding eosin to tissue
I cannot see cons right now, but histoland: please feel free to correct me. Eosin is soluble in water, and as dewaxing most certainly will end in water, the eosin will be washed out by 70% EtOH and distilled water. Check your eosin to be sure. Am 07.12.2011 17:17, schrieb Carol Bryant: What are the pros and cons of adding eosin to tissue as it is processed to increase visibility when embedding with small specimens? Thank you in advance for your input. Carol Bryant, CT (ASCP) Cytology/Histology Manager Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cb...@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] antibody who's who?
Hi! Why not just call the companies and ask them? On 28.10.2011 21:31, Jason Madore wrote: Hello, We are in the process of testing multiple antibodies against the same target(s). I can't figure out though if two companies are selling the same antibody, or rather, as I know they are repackaging and selling the same antibodies, I can't tell who selling who's antibody. If anyone has any suggestions they would be greatly appreciated. Jason ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Drying oven temps
59 °C, 1h, for all slides. Is anyone willing to share their slide drying temperature and times? Does anyone know of a particular standard concerning this? Kimberly Artim, AST, HT (ASCP) Technical Coordinator, Anatomic Pathology St Lukes Hospital Health Network 801 Ostrum Street Bethlehem, PA 18015 610-954-4832 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] hematoxylin washed off
Is it only the HTX which is pale? Did you change anything else? Like dewaxing? anuradha shrivastava: Hello Every body, We changed the solutions testerday in the processor, and today Dr. complained about pale hematoxylin. Can u suggest what is wrong. thanks. anu. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Histonet - unsubscribe
Okay, bye bye :) But do not forget to visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet and fill out the form on the bottom of the page! Unsubscribe from Histonet. Thank you Georgia Stewart ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] thermometer
A pyrometer should do the job, I guess. My last CLIA inspection, I was told to check the temperature in the wax chamber of my embedding station to compare against the temperature it says it is...does anyone know of a good thermometer to purchase? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re:2
Redirects to a site which offers pills (I cannot even see what pills...) ...I hope you’ll enjoy after visiting this site. http://kristiang.kr.funpic.de/page.php?mafortune=65m5 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] OT: April Fool's Nonsense
http://www.youtube.com/watch?v=tPifEfo0GuI ? This might result in permanent damage to YOU as well, I guess... Remove the little wheels under the chairs? Mostly they are just plugged in and can easily be removed. Gelatine in the coffee maker? Whatever you do - make sure the list will know the results somehow ;) I like what I've gotten so far but I'm obviously a lot more evil than some of you... I'm thinking about how I could temporarily glue the radio station dial on the boss's radio to classical music in order to keep him from listening to Rush Limbaugh and Glen Beck. Or Saran Wrap over the urinal. Vaseline on office door handles. Filling one of their offices with bubble wrap. Gluing their desk drawer shut (the one with the food in it). Let's get SERIOUS here - I need more! I want people to be glad I've retired! Heh...heh... No pathologists were harmed in the making of this Tomfoolery. Darn it. And, yes, I do have work to do today... so far, anyway. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Please add
Almost looks like Friday :-D Dear Lucie, please go to http://lists.utsouthwestern.edu/mailman/listinfo/histonet and fill out the form at the bottom of the page to subscribe. Regards ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Need help with interpretation: Gomori Trichrome / MELAS-Syndrom
Hi all, I'm reading about OXPHOS diseases (defects in mitochondrial protein subunits of respiratory chain). RFF in MERRF stands for red ragged fibers, see: http://en.wikipedia.org/wiki/File:Ragged_red_fibers_in_MELAS.jpg I don't get what is stained there exactly. The picture shows a muscle biopsy stained with Gomori's trichrome stain.. What is stained in these red fibers? I'm sure one of you has some experience with that. Thanks, V. Neubert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] glass cleaner
~ 90 mL 70% EtOH ~ 10 mL 25% HCl Removed everything I ever had to deal with. After that give your glassware a good wash in the dishwasher, then rinse with plenty of demineralized water. And wear protective gloves, but as we all do, I actually don't have to remind any of the list members, right? ;) Hello I am looking for suggestions on the best chemical cleaner for glassware that is used for special stains. Any ideas? Thanks, Nacaela Johnson Histology Technician KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: nacaela.john...@usoncology.com /preThe contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.brOnly the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone./pre ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] (no subject)
Is there a chance to go and see a lab which does histology? Hello I am really new to the field of histlogy. I wanted to get some opinions on this field vs. cytology. I am wondering which field is better for me. Any suggestions or thoughts are welcome. Thanks!!! I remain yours truely, Candice Camille ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Looking for picture of urinary bladder, murine
Hello Histoland, if there is anyone who can send me a digital picture of murine urinary bladder, HE stained, Bouin-Hollande fixed if possible - please do so! Thank you, Valentin ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Problems with listserv
YMMD! You are the first I notice who wants to subscribe, not unscibe or unscribe or unsubscribe :D http://lists.utsouthwestern.edu/mailman/listinfo/histonet I am having some issues with receiving email from the listserv. I was successfully receiving email and it suddenly stopped. If I have been unsubscribed, please re subscribe my email address. sfe...@cmc-nh.org Thank you, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfe...@cmc-nh.org mailto:sfe...@cmc-nh.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] coated slides
Hi, you could buy Super Frost Plus slides. If you want to coat slides by yourself, you could buy poly-L lysine 0.1mg/mL. Put a drop of about 5µL on the left upper corner of a slide, then put two of those slides together (surface on surface), move them around until the lysine is spread all over the slides, then seperate them by just pulling without letting any air between the slides. Easy :) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Water collecting at bottom of sections
I experienced it almost daily and thought this was normal!? I just dipped the slides gently on a paper towel right after getting them on the slide and shaking, for the case I could not just shake it off. Indeed, as I remember, the Super Frost Plus slides had to be dipped a bit harder to let the water rinse off. Hello all, From time to time and depending on what brand of adhesive (or charged) slides I am using, I seem to get a bag of water that drains to the bottom of my sections but doesn't drain out. I have been working in microtomy a long time and have had to deal with this contingency time and time again, but never really have gotten to the bottom of the problem. I spoke with a premium manufacturer of such slides and they seemed to indicate that it is a problem with the coating, but couldn't tell me for sure. All I know is that certain brands do this more than others. If you know what I mean, you know it is a problem. My bath is pure distilled H2O with no gelatin or Sta-on added. It is if the adhesive properties are SO good that they will not release the water when vertically drained and have to be shaken off or cut with a razor blade at bottom to release the water. Anyway, if anyone has an insight or two on this, I would be interested. It seem sthe most challenging issues are ones that seem related to some of the most simple tasks that one has performed for many years!! Manufacturers understand what I mean, but cannot pinpoint the problem for me via phone or e-mail. Anyone see this and have a chemical/mechanical solution they have developed over the years? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] bat wing histology
Make that an open discussion on the list, no under cover posting if possible, please! :) Hello everyone, This is my very first post and I am desperately looking for help. I am new to histology, so any help would be much appreciated. I am studying the peripheral sensory innervation of bat wings. As a first step, I would like to demonstrate the innervation pattern on the different parts of the wing membrane (a whole mount of the wing?). Second, I would like to demonstrate the mechanoreceptor make-up of the tiny hairs on the wing membrane. Bat wings are highly elastic, with numerous folds, a thickness of about 35-45 microns (in the species I study), a network of thin collagen bundles, and pigmented superficial epidermal layers. I could provide more information if required. Hoping to hear back from the members. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] slim jims
I just found a partially eaten Slim Jim snack picture on wikipedia... Is it Friday again? :- Do you put the slim jims in formalin and then process them or just put them in the processor? Margaret ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Yellow counterstain
I'd use tartrazine or picric acid mixed with acetone. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Xylene substitutes
My hand staining setup contained some commercial naphta mix, which is comparable to any other petroleum benzine solvant, for dewaxing. For dehydrating/coverslipping, I used n-Butyl acetate a.k.a. butyl ethanoate. Coverslipping can be done with any xylene soluble glue. What are labs using for xylene substitute. I will be using it in a hand staining setup for Mohs. Feed back on Histo-Clear, Clear solve, S-3 and formular 83. Thanks in advance. Dawn Oakes HT - Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] keep deparaffinized and rehydrated slides in dH2O for a few days
No, I would not do that. Why don't you dehydrate again an put some drops of paraffin on it? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Please include me at your mailing list. Thank you.
Come on, guys! Who's the first to post Click link at bottom of mail!? :D Please include me at your mailing list. Thank you. Dima ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] sliver help
Stained yourself? Nothing to be ashamed of! Wo gehobelt wird, da fliegen Späne German saying :) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] spinal cord paraffin embedding
Hi, I processed small porcine spinal cord once (diameter ~3-5mm). My pathologist did not mention any abnormalities, so I guess our standard protocol for every type of tissue was fine. for 1h each: NBF 70% isopropanol ( = 2-propanol) 80% isopropanol 95% isopropanol 100% isopropanol 100% isopropanol 100% isopropanol xylene xylene paraffine paraffine If nothing else helps, maybe you want to give that a try with slightly shorter times in the alcoholic solutions, as your specimen seems to be smaller in size. Dear all, I'm quite new in morphological analysis and Histology. In my l=ab we are processing rat spinal cord to perform luxol fast blue staining. First we fix the spinal cord (only lumbar portion) in 4% parafolmaldehyde (4h) =y immesion, then the samples are embedded in paraffin using a authomatic Leica ASP300 machine. When observed, the white matter of the spinal cord is full of holes and myelin seems to be absent. I guess the problem was related to the processing steps=erformed by the machine, in particular the Xylol step. Is there someone who can help me sharing his/her experience on spinal cord process=ng protocol? Any suggestion will be highly appreciated. Regards -- Dott.ssa Elisa Ballarini,PhD student Dipartimento di Neuroscie=ze e Tecnologie Biomediche Università degli Studi Milano-Bicocca Via C=dore 48-20052,Monza,MB e.ballari...@campus.unimib.it Tel. 02-64488119 Fax. 02-64488253 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Am 15.03.2010 15:05, schrieb Elisa Ballarini: Dear all, I'm quite new in morphological analysis and Histology. In my l=ab we are processing rat spinal cord to perform luxol fast blue staining. First we fix the spinal cord (only lumbar portion) in 4% parafolmaldehyde (4h) =y immesion, then the samples are embedded in paraffin using a authomatic Leica ASP300 machine. When observed, the white matter of the spinal cord is full of holes and myelin seems to be absent. I guess the problem was related to the processing steps=erformed by the machine, in particular the Xylol step. Is there someone who can help me sharing his/her experience on spinal cord process=ng protocol? Any suggestion will be highly appreciated. Regards -- Dott.ssa Elisa Ballarini,PhD student Dipartimento di Neuroscie=ze e Tecnologie Biomediche Università degli Studi Milano-Bicocca Via C=dore 48-20052,Monza,MB e.ballari...@campus.unimib.it Tel. 02-64488119 Fax. 02-64488253 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC TBS wash buffer
7,6 ;) Can anyone tell me what an acceptable pH range for IHC TBS wash buffer is? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] light staining
Check pH of your washing water. pH too low will result in not staining at all. I'd clean the stainer as well. Hello Histonetters We are having trouble with light staining from the hematoxylin. It is the same lot number we have been in. We changed it out and still the same. All reagent containers were emptied, cleaned and refilled yesterday. We have not changed anything with the timings on our stainer. What am I missing? Thanks for your help Nancy Schmitt United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Tips for drying slides before HE stain.
58°C for 45-60min, slides in an upright position. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Slide marking pens
Should've kept it for Friday's fun :-D Anyway, I guess that there is no ink that will withstand several hours in both watery and organic solvants. I used Securline Lab Markers II, even those will faint slightly when processing. Can be scrubbed of with a HCl-ethanole-water solution. but the ink still comes off! Are these pens for this purpose? Or can you recommend a good marking pen for us? yep... a pencil ;) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Slide marking pens
Keep the cap on the pen and always store the pen on its tip (even in the lab coats pocket). Won't dry out, for sure. I have used Secureline Markers as one person suggested, but they seemed to dry up fast. Peggy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Eliminating the edge effect in IHC/IF
Is it just unevenly stained? Or are your sections unevenly thick!? Hi, I have a question for the generous input. When I do the IHC or IF, it seems very common that the intensity of the edge area of the tissue is always stronger than the central tissue part. Is it possible to eliminate this and make the staining evenly distributed around the whole tissue section? Your kind help is greatly appreciated, Thanks in advance, Best, Karen Karen Cai Research Scientist Prosci Incorporated (858) 513-2638 x 204 (858) 513-2692 Fax http://www.prosci-inc.com www.prosci-inc.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] leaving IHC slides in wash buffer
I guess yes. Unless I can say exactly which ingredient will affect my staining, I would not leave them in any solution for such a long time! Hi Everyone, I have yet another IHC-related question. So after antigen retrieval, and then allowing my slides too cool, I place them in wash buffer before loading them on to the autostainer. Would leaving the slides in the wash buffer in the fridge for an extended period of time (say, 1 week) before continuing with immunostaining affect staining quality in any way? Thank you, Jen Campbell ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] slide handling for special stains
I don't see any point in leaving deparaffinized and rehydrated(!) slides any longer in water. The tissue might even fall off. What special stains are you goint to do? Hello all, I need some information on special stains in regards to handling of slides. Would it be good practice to deparaffinize and rehydrate slides then allow them to sit in water over night or over the weekend before doing the actual staining? Thanks Roger Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 rchar...@state.pa.usmailto:rchar...@state.pa.us No trees were hurt in the sending of this email, However many electrons were severely inconvenienced! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Plants in Histology lab
Dish washer dude :-D That's a really awesome story, almost too much clichée to be true. Best Friday spam topic anyway. Bader Siddiki schrieb: Everybody is writing about the plants in the histology lab. May be I will share my experience with you in Biochemistry lab at MSU . Many many years ago when I was a post doc in Biochemistry dept. I had lots of plants in the window. One day when I came from lunch, I saw unusual plants along with mine, on close look, they were marijuana plants. I almost had heart attack to see these plants in my cubicle, right away I hid these plants. On investigation we found that our dish washer had these plants some place in the lab. and she was hiding it from the professor. Bader ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Please remove me from the list
I'm sure this will work from your iPhone too: http://lists.utsouthwestern.edu/mailman/listinfo/histonet Follow the instructions on the bottom of the page for unsubscribing :) Why leave Histonet, anyway? Have a nice weekend, Valentin Angela Riggin wrote: Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] xylene substitues
N-butyl acetate aka butyl ethanoate. njoydo...@aol.com wrote: just wanting to see what everyone's favorite xylene substitute is for clearing slides before coverslipping?? Gene Cleveland Clinic ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Destain Hematoxylon
Hi, I want to destain hematoxylin (Shandon's Gill II). How to do that? Thanks in advance, V. Neubert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] paraffin embedding
Take some paraffin into the mould, then grab your tissue, press it to the bottom, hold it and get onto cooling plate. Wait a few seconds for the paraffin to start getting solid, then fill up with hot paraffin. I use this technique everyday. Ryan McAdams wrote: I am trying to embed adult rat aortas in paraffin for histology purposes. Do you have any recommendations on an optimal method to keep the aortas in a vertical position while the paraffin solidifies? I am hoping to do transverse sectioning of the thoracic aortas to analyze wall thickness. Thanks, Ryan Ryan McAdams Assistant Professor of Pediatrics Division of Neonatology University of Washington Box 356320 Seattle, WA 98195-6320 Telephone: (206)-616-8246 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] email
You can do that on your own - see http://lists.utsouthwestern.edu/mailman/listinfo/histonet Spam stopped - why not stay and share histo knowledge? :) Nan Wang wrote: Hi, Can you remove me from the email list? Too many emails. Thanks. nan wang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] To all spam gourmets
Please, stop spamming this list. Comment if you know something useful. Otherwise keep it. I don't mind what you had for breakfast in June 1st last year or your opinion on my clothes' colour. Believe it or not, there IS a real life ( link: http://en.wikipedia.org/wiki/Real_life_(reality) ), and maybe you should check it out - it has way more to offer than waiting for responses to mock about. Thanks. Valentin PS: What about a moderated board? Every inappropriate comment on HISTO TOPIC could be moved or even deleted, except in the spamming forum which can be found on nearly every board on the net. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] So... I Need Some Expert Advice:
I suggest to start a board/forum. Really. On Wed, 1 Apr 2009 23:13:09 -0700 (PDT), aa aa lactose.intoler...@yahoo.com wrote: Dear Histotech Experts! I am working on a very complicated project, and could really use some advice from both the guru-level histologists and also from the go-getter know-how of the histotechs Because it's sort of hard to explain (and involves some comparatively obscure staining techniques and mounting methods) I've put together a short slide-show to help cut-to-the-chase, as it were, because frankly, some things are just too tedious, abstract and confusing to type out in text. Anyway, on with the show! I would truly and thankfully appreciate any and all commentary and advice! Please view my detailed presentation here: http://tinyurl.com/2g9mqh Thanks Everybody! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Strange circles in IHC slides
Hello, I really have some real links to real pictures of real relevance of the real histonet list. Really promised. http://img12.imageshack.us/img12/8513/ts0402162049.jpg http://img13.imageshack.us/img13/6514/ts0402162104.jpg So, has ever anyone experienced sth. like this? My conjugate control (every step except the antibody) was fine, nothing to be seen about DAB and no circles at all. I used Shandon single-use coverplates, sterile buffer, fresh antibody aliquots. Any idea? Thanks, V. Neubert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] (no subject)
Dear Sahar Darwish, I just had a look into the last 3330 mails I received from this list so far but could not find any mail sent by you. Please make sure you send your requests to histonet@lists.utsouthwestern.edu for making sure it is bounced to every participant. Greetings, V. Neubert Sahar Darwish wrote: dear. respon for histonet: i send many message to other participent in this site ,i have no response, sory iam depressed, i have i question about how to differentiate between islets ccells of pancreas with special stain ,may have an answer please. Dr .sahar kamal researcher in histology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Rehydration // urgent
Hi there, please help immediatly :) I want to rehydrate two specimen which have been processed as follows: 1h NBF 1h 70% 2-propanol (isopropanol) 1h 80% 2-propanol I need to go back to NBF as I want to add some more specimen but can process only one basket at one time (I'm using Microm STP120). Thanks for your replies! V. Neubert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microm HM325 users
Hello Histonetters! Is anyone using a Microm HM325 microtome? Please contact me if you want to share some tricks and useful hints, concerning sectioning to me :) I don't have a question in particular, just want to see if anyone is doing sth like cooling the blade or so. Bye, V. Neubert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Dilution of acetone
Dear Histonetters, to which level can I dilute acetone with water? Any chance to get it to 50% acetone / 50% a. dest. or even further? Thank you for replying ASAP :) Have a nice weekend, Valentin ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microm STP120 and 2-propanol protocol
Hello Histonetters, is there anyone with a Microm ST 120 processor? What protocol do you use? Even one with 2-propanol? Thanks for replying, V. Neubert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Removal magic
Good people, start to read this post and experience the magic which is IN YOUR HANDS! Follow the instructions given on http://lists.utsouthwestern.edu/mailman/listinfo/histonet! YES YOU CAN! Go to the bottom of the page, enter your mail address and follow the wizard :-) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Staining fungi with Warthin-Starry
Just a quick question: Did anyone successfully try to stain fungi with Warthin-Starry silver technique? Thank you for our answers, V. Neubert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Warthin-Starry // Best before dates
Hello! I use the Warthin-Starry technique to stain spirochaetes in FFPE sections. I don't know how long I can keep the AgNO_3 solutions and the hydrochinone solution whitout getting them to expire. Is there anyone who already had to deal with that question? Regards, Valentin ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Gram stain on tissue
Hello! To introduce myself: I've been reading this list for the last ~350 mails. I am a technical assistent, working in vet histo. I am quite inexperienced with staining, and one task is to get Gram staining to work. Right now, I've been trying to get results for 2! days, I tried about 10 different ways. If someone could share a method which has proven to be working, I'd be very happy. Reagents available to work with right now: xylene, acetone, picric acid, carbol fuchsin, basic fuchsin, safranine, fuchsin 1:10. I'd be glad if I had not to order any more extra reagents. Thank you for replying. Greets from Germany, Valentin __ Run, Fatboy, Run sowie Rails Ties kostenlos anschauen! Blockbuster-Gutscheine sichern unter http://www.blockbuster.web.de ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
