Re: [Histonet] Oil Red O

[Niihori, Maki - (mniihori) via Histonet]

Hello John

Thank you very much for the great source of information!

Maki

From: John Kiernan 
Sent: Saturday, March 6, 2021 9:55 AM
To: histonet@lists.utsouthwestern.edu ; 
Niihori, Maki - (mniihori) 
Subject: [EXT]Re: Oil Red O


External Email

It's in any textbook in the field of histotechnology published since about 
1950. This one from Amazon costs less than $8. Every lab should have a shelf of 
such books.

https://www.amazon.com/Introduction-Histotechnology-Geoffrey-G-Brown/dp/0838543405
[https://images-na.ssl-images-amazon.com/images/I/41xQTLdptPL.jpg1z]m]<https://www.amazon.com/Introduction-Histotechnology-Geoffrey-G-Brown/dp/0838543405>
An introduction to histotechnology: A manual for the student, practicing 
technologist, and resident-in-pathology: Brown, Geoffrey G: 9780838543405: 
Amazon.com: 
Books<https://www.amazon.com/Introduction-Histotechnology-Geoffrey-G-Brown/dp/0838543405>
An introduction to histotechnology: A manual for the student, practicing 
technologist, and resident-in-pathology [Brown, Geoffrey G] on Amazon.com. 
*FREE* shipping on qualifying offers. An introduction to histotechnology: A 
manual for the student, practicing technologist, and resident-in-pathology
www.amazon.com
John Kiernan
London, Canada
= = =

From: Niihori, Maki - (mniihori) via Histonet 

Sent: March 5, 2021 4:08 PM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Oil Red O

We would like to stain Right Ventricle (RV) and Lung (both from rat samples) 
with Oil Red O.
I appreciate if anybody can share a good protocol/kit information with me.

Thank you,
Maki


*

Maki Niihori, PhD

Life Sciences North Rm# 402,

Department of Medicine,

The University of Arizona

Phone: 520-626-6092

E-mail: mniih...@arizona.edu

*

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Re: [Histonet] Oil Red O

[John Garratt via Histonet]

In Histological and Histochemical Methods in Theory and Practice by John 
Kiernan there is a good method for Oil Red O.

There is definitely an art to a good Oil Red O that is passed down from the 
older experienced tech to the young folks in the lab.

Bancroft’s Theory and Practice of Histological Technique is also a good source 
for staining methods.

John

On Sat, Mar 6, 2021 at 8:55 AM, John Kiernan via Histonet 
 wrote:

> It's in any textbook in the field of histotechnology published since about 
> 1950. This one from Amazon costs less than $8. Every lab should have a shelf 
> of such books.
>
> https://www.amazon.com/Introduction-Histotechnology-Geoffrey-G-Brown/dp/0838543405
> [https://images-na.ssl-images-amazon.com/images/I/41xQTLdptPL.jpg1z]m]<https://www.amazon.com/Introduction-Histotechnology-Geoffrey-G-Brown/dp/0838543405>
> An introduction to histotechnology: A manual for the student, practicing 
> technologist, and resident-in-pathology: Brown, Geoffrey G: 9780838543405: 
> Amazon.com: 
> Books<https://www.amazon.com/Introduction-Histotechnology-Geoffrey-G-Brown/dp/0838543405>
> An introduction to histotechnology: A manual for the student, practicing 
> technologist, and resident-in-pathology [Brown, Geoffrey G] on Amazon.com. 
> *FREE* shipping on qualifying offers. An introduction to histotechnology: A 
> manual for the student, practicing technologist, and resident-in-pathology
> www.amazon.com
> John Kiernan
> London, Canada
> = = =
> 
> From: Niihori, Maki - (mniihori) via Histonet 
> 
> Sent: March 5, 2021 4:08 PM
> To: histonet@lists.utsouthwestern.edu 
> Subject: [Histonet] Oil Red O
>
> We would like to stain Right Ventricle (RV) and Lung (both from rat samples) 
> with Oil Red O.
> I appreciate if anybody can share a good protocol/kit information with me.
>
> Thank you,
> Maki
>
> *
>
> Maki Niihori, PhD
>
> Life Sciences North Rm# 402,
>
> Department of Medicine,
>
> The University of Arizona
>
> Phone: 520-626-6092
>
> E-mail: mniih...@arizona.edu
>
> *
>
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Re: [Histonet] Oil Red O

[John Kiernan via Histonet]

It's in any textbook in the field of histotechnology published since about 
1950. This one from Amazon costs less than $8. Every lab should have a shelf of 
such books.

https://www.amazon.com/Introduction-Histotechnology-Geoffrey-G-Brown/dp/0838543405
[https://images-na.ssl-images-amazon.com/images/I/41xQTLdptPL.jpg1z]m]<https://www.amazon.com/Introduction-Histotechnology-Geoffrey-G-Brown/dp/0838543405>
An introduction to histotechnology: A manual for the student, practicing 
technologist, and resident-in-pathology: Brown, Geoffrey G: 9780838543405: 
Amazon.com: 
Books<https://www.amazon.com/Introduction-Histotechnology-Geoffrey-G-Brown/dp/0838543405>
An introduction to histotechnology: A manual for the student, practicing 
technologist, and resident-in-pathology [Brown, Geoffrey G] on Amazon.com. 
*FREE* shipping on qualifying offers. An introduction to histotechnology: A 
manual for the student, practicing technologist, and resident-in-pathology
www.amazon.com
John Kiernan
London, Canada
= = =

From: Niihori, Maki - (mniihori) via Histonet 

Sent: March 5, 2021 4:08 PM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Oil Red O

We would like to stain Right Ventricle (RV) and Lung (both from rat samples) 
with Oil Red O.
I appreciate if anybody can share a good protocol/kit information with me.

Thank you,
Maki


*

Maki Niihori, PhD

Life Sciences North Rm# 402,

Department of Medicine,

The University of Arizona

Phone: 520-626-6092

E-mail: mniih...@arizona.edu

*

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Re: [Histonet] Oil Red O

[John Garratt via Histonet]

What have you fixed the tissue with? Can you cut frozen sections from the fixed 
tissue?
This is useful information to start with.

John

On Fri, Mar 5, 2021 at 1:08 PM, Niihori, Maki - \(mniihori\) via Histonet 
 wrote:

> We would like to stain Right Ventricle (RV) and Lung (both from rat samples) 
> with Oil Red O.
> I appreciate if anybody can share a good protocol/kit information with me.
>
> Thank you,
> Maki
>
> *
>
> Maki Niihori, PhD
>
> Life Sciences North Rm# 402,
>
> Department of Medicine,
>
> The University of Arizona
>
> Phone: 520-626-6092
>
> E-mail: mniih...@arizona.edu
>
> *
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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[Histonet] Oil Red O

[Niihori, Maki - (mniihori) via Histonet]

We would like to stain Right Ventricle (RV) and Lung (both from rat samples) 
with Oil Red O.
I appreciate if anybody can share a good protocol/kit information with me.

Thank you,
Maki


*

Maki Niihori, PhD

Life Sciences North Rm# 402,

Department of Medicine,

The University of Arizona

Phone: 520-626-6092

E-mail: mniih...@arizona.edu

*

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Re: [Histonet] Oil Red O permanent mountant

[Hagon, Christopher (Health) via Histonet]

UNCLASSIFIED

Hi again,

Meant to say ThermoFisher Aqua Mount, not Permount in my previous post.

Apologies,

Chris Hagon | Senior Scientist, Anatomical Pathology
ACT Pathology | health.act.gov.au
Phone (02) 5124 2874



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[Histonet] Oil Red O

[Amos Brooks via Histonet]

Hi,
 I have seen this too. I mitigate the problem by making it up in 50 ml
increments and staining them flat. I draw from around the middle of the
Falcon tube I make it up in. It tends to precipitate so you could filter
it. Don't bother re-using the reagent. It's cheap & easy to make up. I
usually only see the precipitate with old solutions. Just make what you use
within a few weeks.

Best 'o luck,
Amos



>
> Message: 2
> Date: Wed, 3 Oct 2018 15:26:25 +
> From: Reuel Cornelia 
> To: "histonet@lists.utsouthwestern.edu"
>         
> Subject: [Histonet] Oil Red O
> Message-ID:
> <
> sn1pr17mb0543f87dbc5e39aa8875c9f1e7...@sn1pr17mb0543.namprd17.prod.outlook.com
> >
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> I was wondering if you could help me know why our Oil Red O have some
> black snowflakes on our fat tissue hours after staining or after 24 hours
> more snowlflakes precipitation occurs.  The staining was reference was from
> Lillie RD, Ashburn. Please note that our staining works well between two to
> three hours. Is there a reason fro this precipitation?
>
>
> Reuel
>
> TSRH
>
> Dallas, TX
>
>
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Re: [Histonet] Oil Red O

[Caroline Miller via Histonet]

Hi Reuel,

In my experience this is the ORO crystallizing out on the section. I would
first filter your staining solution, then keep your staining times to as
short as you can. Also changing between 37 and room temperatures may be
useful too.

mills
ᐧ

On Wed, Oct 3, 2018 at 8:39 AM Reuel Cornelia via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> I was wondering if you could help me know why our Oil Red O have some
> black snowflakes on our fat tissue hours after staining or after 24 hours
> more snowlflakes precipitation occurs.  The staining was reference was from
> Lillie RD, Ashburn. Please note that our staining works well between two to
> three hours. Is there a reason fro this precipitation?
>
>
> Reuel
>
> TSRH
>
> Dallas, TX
>
> --
> Texas Scottish Rite Hospital for Children is one of the nation's leading
> pediatric centers for the treatment of orthopedic conditions, certain
> related neurological disorders and learning disorders, such as dyslexia.
> This email transmission and/or its attachments may contain confidential
> health information, intended only for the use of the individual or entity
> named above.
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-- 
Caroline Miller (mills)
Director of Biology

www.3Scan.com
415 2187297
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[Histonet] Oil Red O

[Reuel Cornelia via Histonet]

I was wondering if you could help me know why our Oil Red O have some black 
snowflakes on our fat tissue hours after staining or after 24 hours more 
snowlflakes precipitation occurs.  The staining was reference was from Lillie 
RD, Ashburn. Please note that our staining works well between two to three 
hours. Is there a reason fro this precipitation?


Reuel

TSRH

Dallas, TX

--
Texas Scottish Rite Hospital for Children is one of the nation's leading
pediatric centers for the treatment of orthopedic conditions, certain 
related neurological disorders and learning disorders, such as dyslexia. 
This email transmission and/or its attachments may contain confidential 
health information, intended only for the use of the individual or entity
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[Histonet] Oil Red O

[Bernice Frederick via Histonet]

Hello all,
Will Oil Red O stain phospholipids? We will be helping another lab with some 
work and the project out line states that Phopslipids are externalized on the 
cell surface during apoptosis. Can always try it I suppose.
Bernice

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-freder...@northwestern.edu

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[Histonet] Oil Red O

[Bitting, Angela K.]

Hello Fellow Histonetters,

We often get slides from another hospital for Oil Red O staining. On occasion, 
they send them at room temp. and until they get to us it's been a day or more.
I'm worried that because they are unfixed the cells will be autolyzed and I 
don't want to charge the patient for a useless stain.
  I haven't been able to find any guidance on how long a frozen section can sit 
at room temp. and still be acceptable to stain for Oil Red O.
Your thought and opinions please?

Angie




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[Histonet] Oil Red O staining question

[David Burk]

Experts!

 

Is there any reason you would not consider formalin fixation followed by
cryoprotection/cryosectioning for Oil Red O (or bodipy for that matter)
staining of mouse muscle? 

I ask as many folks seem to have difficulty in the flash freezing aspect
of tissue collection and end up with lots of ice crystal damage. While
the above method is still subject to freezing artifact it does at least
negate the 'rushed collection' part of the process.

 

The literature favors flash frozen tissue but we've done successful
staining of liver that has been fixed then cryoprotected prior to
staining and things looked fine. Is there an issue with tissue adhering
to slides after sectioning or some other reason this method is not
preferred?

 

I value your opinion as I am wondering why one would choose one approach
over the other.  Let's pretend tissue antigenicity isn't a factor - only
section quality and lipid droplet staining.

 

Thanks for your comments!

 

David

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[Histonet] oil red o on cytology

[Horn, Hazel V]

What kind of control do you use when looking for fat in a BAL specimen?  This 
stain is done on a cytospin slide.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.orgmailto:hor...@archildrens.org
archildrens.orghttp://www.archildrens.org/







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RE: [Histonet] Oil red O

[P.E. Visser]

Dear Rene, Jennifer and others 

my question is as Jennifer MacDonald suggested

I may agree that the question is irrelevant but the question was rased and I
have no clue.

Regards Piet visser

 

 

Van: Rene J Buesa [mailto:rjbu...@yahoo.com] 
Verzonden: maandag 9 september 2013 21:59
Aan: Jennifer MacDonald
CC: histonet@lists.utsouthwestern.edu;
histonet-boun...@lists.utsouthwestern.edu; P.E. Visser
Onderwerp: Re: [Histonet] Oil red O

 

It is for Sudan Red 5B. All these apothecary names are essentially
meaningless

René J.

 

From: Jennifer MacDonald  mailto:jmacdon...@mtsac.edu
jmacdon...@mtsac.edu
To: 
Cc:  mailto:histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu 
mailto:histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu;
mailto:histonet-boun...@lists.utsouthwestern.edu
histonet-boun...@lists.utsouthwestern.edu; P.E. Visser 
mailto:p...@xs4all.nl p...@xs4all.nl 
Sent: Monday, September 9, 2013 3:53 PM
Subject: Re: [Histonet] Oil red O


I believe the question is what is the O signify.
as in OG6 the O is for orange.



From:  Rene J Buesa rjbu...@yahoo.com
To:P.E. Visser p...@xs4all.nl, histonet@lists.utsouthwestern.edu 
histonet@lists.utsouthwestern.edu
Date:  09/09/2013 12:46 PM
Subject:Re: [Histonet] Oil red O
Sent by:histonet-boun...@lists.utsouthwestern.edu



Do you mean that you were asked to do Oil Red O (ORO) stain?
It is described in any technology book.
I piece of advise: stain the nuclei with hametoxyline first and after that 
stain with ORO
René J.



From: P.E. Visser p...@xs4all.nl
To: histonet@lists.utsouthwestern.edu 
Sent: Monday, September 9, 2013 3:40 PM
Subject: [Histonet] Oil red O


Hi all

I was requested where the O stands for. who has any suggestion.



Regards Piet Visser 

Histotech Bronovo The Netherlands





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Re: [Histonet] Oil red O

[Grantham, Andrea L - (algranth)]

Rene,
You still have not answered the question - what does the O stand for?




Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algra...@email.arizona.edumailto:algra...@email.arizona.edu
Tel: 520.626.4415 Fax: 520.626.2097





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RE: [Histonet] Oil red O

[Goins, Tresa]

May be the position of the active group on the benzene ring - ortho vs. 
meta vs. para ?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Grantham, 
Andrea L - (algranth)
Sent: Monday, September 09, 2013 2:06 PM
Cc: HISTONET
Subject: Re: [Histonet] Oil red O

Rene,
You still have not answered the question - what does the O stand for?




Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algra...@email.arizona.edumailto:algra...@email.arizona.edu
Tel: 520.626.4415 Fax: 520.626.2097





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Re: [Histonet] Oil red O

[Ada Feldman]

As you are finding out the answers to dye nomenclature you can had these 
endings to your list of the O' in Oil red O:
Oil red EGN
Oil red 4B


Ada Feldman
Anatech Ltd.
1020 Harts Lake Road
Battle Creek, MI 49037

Phone: 800.262.8324
Phone: 269.964.6450
Fax: 269.964.8084
adafeld...@anatechltdusa.com




On Sep 9, 2013, at 3:59 PM, Connolly, Brett M wrote:

 I'm not sure, but whatever you do... don't store it next to the Sudan Black 
 B...you'll stink up the lab!!
 
 Brett M. Connolly, Ph.D.
 Principal Scientist, Imaging Dept.
 Merck  Co., Inc.
 PO Box 4, WP-44K
 West Point, PA 19486
 brett_conno...@merck.com
 T- 215-652-2501
 F- 215-993-6803
 
 
 
 
 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of P.E. Visser
 Sent: Monday, September 09, 2013 3:41 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Oil red O
 
 Hi all
 
 I was requested where the O stands for. who has any suggestion.
 
 
 
 Regards Piet Visser 
 
 Histotech Bronovo The Netherlands
 
 
 
 
 
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Re: [Histonet] Oil red O

[Rene J Buesa]

I wrote that apothecary names are meaningless = they do not have to have a 
specific meaning that, in this case, I do not have the foggiest idea! It does 
not have to be anything specific. 
Something is for sure: it has nothing to do with orange.
 René J.



From: Grantham, Andrea L - (algranth) algra...@email.arizona.edu
To: 
Cc: HISTONET histonet@lists.utsouthwestern.edu 
Sent: Monday, September 9, 2013 4:06 PM
Subject: Re: [Histonet] Oil red O


Rene,
You still have not answered the question - what does the O stand for?




Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algra...@email.arizona.edumailto:algra...@email.arizona.edu
Tel: 520.626.4415    Fax: 520.626.2097





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[Histonet] Oil red O

[P.E. Visser]

Hi all

I was requested where the O stands for. who has any suggestion.

 

Regards Piet Visser 

Histotech Bronovo The Netherlands

 

 

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Re: [Histonet] Oil red O

[Rene J Buesa]

If that is the case, ORO will stain fat droplets in Frozen Sections. In FFPE 
will stain nothing.
René J.



From: Mitchell Jean A jmitch...@uwhealth.org
To: 'Rene J Buesa' rjbu...@yahoo.com; P.E. Visser p...@xs4all.nl; 
histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu 
Sent: Monday, September 9, 2013 3:49 PM
Subject: RE: [Histonet] Oil red O


I believe the question is:  what does the O in Oil Red O stand for?


Jean Mitchell, BS HT (ASCP)
University of Wisconsin Hospital  Clinics
Neuromuscular Laboratory 
600 Highland Avenue
Madison, WI  53792-5132 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Monday, September 09, 2013 2:45 PM
To: P.E. Visser; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Oil red O

Do you mean that you were asked to do Oil Red O (ORO) stain?
It is described in any technology book.
I piece of advise: stain the nuclei with hametoxyline first and after that 
stain with ORO René J.



From: P.E. Visser p...@xs4all.nl
To: histonet@lists.utsouthwestern.edu
Sent: Monday, September 9, 2013 3:40 PM
Subject: [Histonet] Oil red O


Hi all

I was requested where the O stands for. who has any suggestion.



Regards Piet Visser 

Histotech Bronovo The Netherlands





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RE: [Histonet] Oil red O

[Connolly, Brett M]

I'm not sure, but whatever you do... don't store it next to the Sudan Black 
B...you'll stink up the lab!!

Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of P.E. Visser
Sent: Monday, September 09, 2013 3:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Oil red O

Hi all

I was requested where the O stands for. who has any suggestion.

 

Regards Piet Visser 

Histotech Bronovo The Netherlands

 

 

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Re: [Histonet] Oil red O

[Rene J Buesa]

It is for Sudan Red 5B. All these apothecary names are essentially meaningless
René J.



From: Jennifer MacDonald jmacdon...@mtsac.edu
To: 
Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu; 
histonet-boun...@lists.utsouthwestern.edu; P.E. Visser p...@xs4all.nl 
Sent: Monday, September 9, 2013 3:53 PM
Subject: Re: [Histonet] Oil red O


I believe the question is what is the O signify.
as in OG6 the O is for orange.



From:  Rene J Buesa rjbu...@yahoo.com
To:    P.E. Visser p...@xs4all.nl, histonet@lists.utsouthwestern.edu 
histonet@lists.utsouthwestern.edu
Date:  09/09/2013 12:46 PM
Subject:        Re: [Histonet] Oil red O
Sent by:        histonet-boun...@lists.utsouthwestern.edu



Do you mean that you were asked to do Oil Red O (ORO) stain?
It is described in any technology book.
I piece of advise: stain the nuclei with hametoxyline first and after that 
stain with ORO
René J.



From: P.E. Visser p...@xs4all.nl
To: histonet@lists.utsouthwestern.edu 
Sent: Monday, September 9, 2013 3:40 PM
Subject: [Histonet] Oil red O


Hi all

I was requested where the O stands for. who has any suggestion.



Regards Piet Visser 

Histotech Bronovo The Netherlands





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Re: [Histonet] Oil red O

[Rene J Buesa]

There you have it!
René J.



From: Ada Feldman adafeld...@anatechltdusa.com
To: histonet@lists.utsouthwestern.edu 
Sent: Monday, September 9, 2013 4:13 PM
Subject: Re: [Histonet] Oil red O


As you are finding out the answers to dye nomenclature you can had these 
endings to your list of the O' in Oil red O:
Oil red EGN
Oil red 4B


Ada Feldman
Anatech Ltd.
1020 Harts Lake Road
Battle Creek, MI 49037

Phone: 800.262.8324
Phone: 269.964.6450
Fax: 269.964.8084
adafeld...@anatechltdusa.com




On Sep 9, 2013, at 3:59 PM, Connolly, Brett M wrote:

 I'm not sure, but whatever you do... don't store it next to the Sudan Black 
 B...you'll stink up the lab!!
 
 Brett M. Connolly, Ph.D.
 Principal Scientist, Imaging Dept.
 Merck  Co., Inc.
 PO Box 4, WP-44K
 West Point, PA 19486
 brett_conno...@merck.com
 T- 215-652-2501
 F- 215-993-6803
 
 
 
 
 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of P.E. Visser
 Sent: Monday, September 09, 2013 3:41 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Oil red O
 
 Hi all
 
 I was requested where the O stands for. who has any suggestion.
 
 
 
 Regards Piet Visser 
 
 Histotech Bronovo The Netherlands
 
 
 
 
 
 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 Notice:  This e-mail message, together with any attachments, contains
 information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
 New Jersey, USA 08889), and/or its affiliates Direct contact information
 for affiliates is available at 
 http://www.merck.com/contact/contacts.html) that may be confidential,
 proprietary copyrighted and/or legally privileged. It is intended solely
 for the use of the individual or entity named on this message. If you are
 not the intended recipient, and have received this message in error,
 please notify us immediately by reply e-mail and then delete it from 
 your system.
 
 
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RE: [Histonet] Oil red O

[WILLIAM DESALVO]

I believe the answer should be - Oil Red O is a brand name (not uncommon in the 
dye indutry) and represents the two dyes found in the compound, Solvent Red 27 
and Sudan Red 5B

William DeSalvo, BS HTL(ASCP)

 Date: Mon, 9 Sep 2013 13:32:08 -0700
 From: rjbu...@yahoo.com
 To: adafeld...@anatechltdusa.com; histonet@lists.utsouthwestern.edu
 Subject: Re: [Histonet] Oil red O
 CC: 
 
 There you have it!
 René J.
 
 
 
 From: Ada Feldman adafeld...@anatechltdusa.com
 To: histonet@lists.utsouthwestern.edu 
 Sent: Monday, September 9, 2013 4:13 PM
 Subject: Re: [Histonet] Oil red O
 
 
 As you are finding out the answers to dye nomenclature you can had these 
 endings to your list of the O' in Oil red O:
 Oil red EGN
 Oil red 4B
 
 
 Ada Feldman
 Anatech Ltd.
 1020 Harts Lake Road
 Battle Creek, MI 49037
 
 Phone: 800.262.8324
 Phone: 269.964.6450
 Fax: 269.964.8084
 adafeld...@anatechltdusa.com
 
 
 
 
 On Sep 9, 2013, at 3:59 PM, Connolly, Brett M wrote:
 
  I'm not sure, but whatever you do... don't store it next to the Sudan Black 
  B...you'll stink up the lab!!
  
  Brett M. Connolly, Ph.D.
  Principal Scientist, Imaging Dept.
  Merck  Co., Inc.
  PO Box 4, WP-44K
  West Point, PA 19486
  brett_conno...@merck.com
  T- 215-652-2501
  F- 215-993-6803
  
  
  
  
  
  
  -Original Message-
  From: histonet-boun...@lists.utsouthwestern.edu 
  [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of P.E. Visser
  Sent: Monday, September 09, 2013 3:41 PM
  To: histonet@lists.utsouthwestern.edu
  Subject: [Histonet] Oil red O
  
  Hi all
  
  I was requested where the O stands for. who has any suggestion.
  
  
  
  Regards Piet Visser 
  
  Histotech Bronovo The Netherlands
  
  
  
  
  
  ___
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  Histonet@lists.utsouthwestern.edu
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  Notice:  This e-mail message, together with any attachments, contains
  information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
  New Jersey, USA 08889), and/or its affiliates Direct contact information
  for affiliates is available at 
  http://www.merck.com/contact/contacts.html) that may be confidential,
  proprietary copyrighted and/or legally privileged. It is intended solely
  for the use of the individual or entity named on this message. If you are
  not the intended recipient, and have received this message in error,
  please notify us immediately by reply e-mail and then delete it from 
  your system.
  
  
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RE: [Histonet] Oil red O

[Morken, Timothy]

Just like Formalin, a brand name now used interchangeably with formaldehyde.

And your trivia of the day:

Formaldehyde was the first polyatomic organic molecule detected in the 
interstellar medium

Courtesy of Wikipedia.

Tim Morken
UCSF Pathology

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO
Sent: Monday, September 09, 2013 2:00 PM
To: Rene J Buesa; Ada Feldman; histonet
Subject: RE: [Histonet] Oil red O

I believe the answer should be - Oil Red O is a brand name (not uncommon in the 
dye indutry) and represents the two dyes found in the compound, Solvent Red 27 
and Sudan Red 5B

William DeSalvo, BS HTL(ASCP)

 Date: Mon, 9 Sep 2013 13:32:08 -0700
 From: rjbu...@yahoo.com
 To: adafeld...@anatechltdusa.com; histonet@lists.utsouthwestern.edu
 Subject: Re: [Histonet] Oil red O
 CC: 
 
 There you have it!
 René J.
 
 
 
 From: Ada Feldman adafeld...@anatechltdusa.com
 To: histonet@lists.utsouthwestern.edu
 Sent: Monday, September 9, 2013 4:13 PM
 Subject: Re: [Histonet] Oil red O
 
 
 As you are finding out the answers to dye nomenclature you can had these 
 endings to your list of the O' in Oil red O:
 Oil red EGN
 Oil red 4B
 
 
 Ada Feldman
 Anatech Ltd.
 1020 Harts Lake Road
 Battle Creek, MI 49037
 
 Phone: 800.262.8324
 Phone: 269.964.6450
 Fax: 269.964.8084
 adafeld...@anatechltdusa.com
 
 
 
 
 On Sep 9, 2013, at 3:59 PM, Connolly, Brett M wrote:
 
  I'm not sure, but whatever you do... don't store it next to the Sudan Black 
  B...you'll stink up the lab!!
  
  Brett M. Connolly, Ph.D.
  Principal Scientist, Imaging Dept.
  Merck  Co., Inc.
  PO Box 4, WP-44K
  West Point, PA 19486
  brett_conno...@merck.com
  T- 215-652-2501
  F- 215-993-6803
  
  
  
  
  
  
  -Original Message-
  From: histonet-boun...@lists.utsouthwestern.edu 
  [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of P.E. 
  Visser
  Sent: Monday, September 09, 2013 3:41 PM
  To: histonet@lists.utsouthwestern.edu
  Subject: [Histonet] Oil red O
  
  Hi all
  
  I was requested where the O stands for. who has any suggestion.
  
  
  
  Regards Piet Visser
  
  Histotech Bronovo The Netherlands
  
  
  
  
  
  ___
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  Histonet@lists.utsouthwestern.edu
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  Notice:  This e-mail message, together with any attachments, 
  contains information of Merck  Co., Inc. (One Merck Drive, 
  Whitehouse Station, New Jersey, USA 08889), and/or its affiliates 
  Direct contact information for affiliates is available at
  http://www.merck.com/contact/contacts.html) that may be 
  confidential, proprietary copyrighted and/or legally privileged. It 
  is intended solely for the use of the individual or entity named on 
  this message. If you are not the intended recipient, and have 
  received this message in error, please notify us immediately by 
  reply e-mail and then delete it from your system.
  
  
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Re: [Histonet] Oil red O

[Rene J Buesa]

Not sure because for that designation to be used there have to be 2 equal 
groups and Oil Red O has 4 benzene rings and in 2 of them there are 4 methyl 
groups in para position so I doubt that the O is for ortho.
rené J.



From: Goins, Tresa tgo...@mt.gov
To: Grantham, Andrea L - (algranth) algra...@email.arizona.edu 
Cc: HISTONET histonet@lists.utsouthwestern.edu 
Sent: Monday, September 9, 2013 4:11 PM
Subject: RE: [Histonet] Oil red O


May be the position of the active group on the benzene ring - ortho vs. 
meta vs. para ?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Grantham, 
Andrea L - (algranth)
Sent: Monday, September 09, 2013 2:06 PM
Cc: HISTONET
Subject: Re: [Histonet] Oil red O

Rene,
You still have not answered the question - what does the O stand for?




Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algra...@email.arizona.edumailto:algra...@email.arizona.edu
Tel: 520.626.4415    Fax: 520.626.2097





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RE: [Histonet] Oil red O

[Mitchell Jean A]

I believe the question is:  what does the O in Oil Red O stand for?


Jean Mitchell, BS HT (ASCP)
University of Wisconsin Hospital  Clinics
Neuromuscular Laboratory 
600 Highland Avenue
Madison, WI  53792-5132 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Monday, September 09, 2013 2:45 PM
To: P.E. Visser; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Oil red O

Do you mean that you were asked to do Oil Red O (ORO) stain?
It is described in any technology book.
I piece of advise: stain the nuclei with hametoxyline first and after that 
stain with ORO René J.



From: P.E. Visser p...@xs4all.nl
To: histonet@lists.utsouthwestern.edu
Sent: Monday, September 9, 2013 3:40 PM
Subject: [Histonet] Oil red O


Hi all

I was requested where the O stands for. who has any suggestion.



Regards Piet Visser 

Histotech Bronovo The Netherlands





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Re: [Histonet] Oil red O

[Jennifer MacDonald]

I believe the question is what is the O signify.
as in OG6 the O is for orange.



From:   Rene J Buesa rjbu...@yahoo.com
To: P.E. Visser p...@xs4all.nl, histonet@lists.utsouthwestern.edu 
histonet@lists.utsouthwestern.edu
Date:   09/09/2013 12:46 PM
Subject:Re: [Histonet] Oil red O
Sent by:histonet-boun...@lists.utsouthwestern.edu



Do you mean that you were asked to do Oil Red O (ORO) stain?
It is described in any technology book.
I piece of advise: stain the nuclei with hametoxyline first and after that 
stain with ORO
René J.



From: P.E. Visser p...@xs4all.nl
To: histonet@lists.utsouthwestern.edu 
Sent: Monday, September 9, 2013 3:40 PM
Subject: [Histonet] Oil red O


Hi all

I was requested where the O stands for. who has any suggestion.



Regards Piet Visser 

Histotech Bronovo The Netherlands





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Re: [Histonet] Oil red O

[Rene J Buesa]

Do you mean that you were asked to do Oil Red O (ORO) stain?
It is described in any technology book.
I piece of advise: stain the nuclei with hametoxyline first and after that 
stain with ORO
René J.



From: P.E. Visser p...@xs4all.nl
To: histonet@lists.utsouthwestern.edu 
Sent: Monday, September 9, 2013 3:40 PM
Subject: [Histonet] Oil red O


Hi all

I was requested where the O stands for. who has any suggestion.



Regards Piet Visser 

Histotech Bronovo The Netherlands





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Re: RE: [Histonet] Oil red O

[E. Wayne Johnson 朱稳森]

While you're at it, maybe some one can explain what the
-wah- in diddy-wah-diddy means?



On 3:59, Morken, Timothy wrote:

Just like Formalin, a brand name now used interchangeably with formaldehyde.

And your trivia of the day:

Formaldehyde was the first polyatomic organic molecule detected in the interstellar 
medium

Courtesy of Wikipedia.

Tim Morken
UCSF Pathology

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO
Sent: Monday, September 09, 2013 2:00 PM
To: Rene J Buesa; Ada Feldman; histonet
Subject: RE: [Histonet] Oil red O

I believe the answer should be - Oil Red O is a brand name (not uncommon in the 
dye indutry) and represents the two dyes found in the compound, Solvent Red 27 
and Sudan Red 5B

William DeSalvo, BS HTL(ASCP)

   

Date: Mon, 9 Sep 2013 13:32:08 -0700
From: rjbu...@yahoo.com
To: adafeld...@anatechltdusa.com; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Oil red O
CC:

There you have it!
René J.



From: Ada Feldmanadafeld...@anatechltdusa.com
To: histonet@lists.utsouthwestern.edu
Sent: Monday, September 9, 2013 4:13 PM
Subject: Re: [Histonet] Oil red O


As you are finding out the answers to dye nomenclature you can had these endings to 
your list of the O' in Oil red O:
Oil red EGN
Oil red 4B


Ada Feldman
Anatech Ltd.
1020 Harts Lake Road
Battle Creek, MI 49037

Phone: 800.262.8324
Phone: 269.964.6450
Fax: 269.964.8084
adafeld...@anatechltdusa.com




On Sep 9, 2013, at 3:59 PM, Connolly, Brett M wrote:

 

I'm not sure, but whatever you do... don't store it next to the Sudan Black 
B...you'll stink up the lab!!

Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of P.E.
Visser
Sent: Monday, September 09, 2013 3:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Oil red O

Hi all

I was requested where the O stands for. who has any suggestion.



Regards Piet Visser

Histotech Bronovo The Netherlands





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contains information of Merck  Co., Inc. (One Merck Drive,
Whitehouse Station, New Jersey, USA 08889), and/or its affiliates
Direct contact information for affiliates is available at
http://www.merck.com/contact/contacts.html) that may be
confidential, proprietary copyrighted and/or legally privileged. It
is intended solely for the use of the individual or entity named on
this message. If you are not the intended recipient, and have
received this message in error, please notify us immediately by
reply e-mail and then delete it from your system.


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[Histonet] Oil red O stain on formalin fixed liver

[Dmborel]

We have an autopsy on a young child.  I have a working diagnosis of possible 
Reye's syndrome and need an Oil Red O stain on a section of formalin fixed 
liver (not processed) to confirm microvesicular hepatic steatosis.  Does anyone 
do this stain, and could I send the tissue or unstained slides for you to 
perform the stain and send islides back for me to interpret?  I can provide a 
bone marrow smear to use as a positive control slide, pay shipping both 
directions and a technical fee.


Thank you,


David M. Borel, M.D.
Pathology Services, P.A.
Topeka, KS
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RE: [Histonet] Oil red O stain on formalin fixed liver

[Tony Henwood (SCHN)]

David,

(From Doug Reye's old Laboratory).

I have found the following technique the best:

Cryotomy of Formalin Fixed Tissues
Sometimes frozen sections are requested on tissue that has been fixed in 10% 
formalin. Cutting frozen sections of formalin fixed tissue can be frustrating 
at times due to shredding of the tissue or failure of the sections to stay on 
the slides. The following procedure makes sectioning a lot easier:

Solutions:

1.  OCT frozen embedding compound (or equivalent)
2.  OCT Infiltration Solution:
OCT Compound4ml
Distilled Water 8ml 
3.  Sticky Slides (Poly Lysine or equivalent)

Method:

1.  Take well-fixed tissue and gently wash in tap water   1hr.
2.  Place in OCT Infiltration Solution and place on rotor.
*   For small pieces (needle or thin wedge) 2hr.
*   For larger pieces 4hrs - overnight.
3.  Gently blot excess solution from tissue and embed in OCT and freeze.
4.  Cut frozen sections and pick up on adhesive coated slides.
5.  Place one slide in Methanol and stain HE or air-dry sections for oil 
red O staining

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager  Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dmborel
Sent: Tuesday, 29 January 2013 6:10 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Oil red O stain on formalin fixed liver

We have an autopsy on a young child.  I have a working diagnosis of possible 
Reye's syndrome and need an Oil Red O stain on a section of formalin fixed 
liver (not processed) to confirm microvesicular hepatic steatosis.  Does anyone 
do this stain, and could I send the tissue or unstained slides for you to 
perform the stain and send islides back for me to interpret?  I can provide a 
bone marrow smear to use as a positive control slide, pay shipping both 
directions and a technical fee.


Thank you,


David M. Borel, M.D.
Pathology Services, P.A.
Topeka, KS
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RE: [Histonet] Oil Red O

[Monfils, Paul]

Oil Red O slides will never fade.  The technique isn't like typical
histochemical procedures where dye molecules bind chemically to tissue
components.  In Oil Red O staining there isn't any chemical binding that
can be reversed.  What you end up with is a solution of the dye in the
fat droplets.  Once coverslipped with an aqueous mounting medium, the
dye cannot escape from the fat droplets because it is insoluble in
water.  Therefore the dye in the fat droplets is just as stable as the
dye solution in the bottle, and the slides will remain good as long as
the coverslipping medium doesn't dry out - which in this case is the
reason for refrigeration, to slow the drying of the mounting medium.



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[Histonet] Oil Red O

[Daniela Bodemer]

Hi all,

 

I am staining cryo sections of aorta for lipids with Oil Red O and
mounting my slides with Mowiol. 

My question is how long will they last without fading and should they be
kept in a folder in the fridge?

 

Thanks for your thoughts,

 

Daniela

 

Daniela Bodemer 

Research Assistant

Surgical Research, Infection and Immunity

 

Murdoch Childrens Research Institute

The Royal Children's Hospital

Flemington Road Parkville Victoria 3052 Australia 

T 03 9936 6676 T (03 9936 6794) 

E daniela.bode...@mcri.edu.au mailto:firstname.surn...@mcri.edu.au 

www.mcri.edu.au http://www.mcri.edu.au/ 

 

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RE: [Histonet] Oil red O versus Sudan 4

[Tony Henwood (SCHN)]

Candice,

Remember freshly prepared Oil Red O Stock usually does not work.
Prepare your saturated Oil Red O Stock and leave it for a week or more before 
using it to prepare a working solution.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager  Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Candice Smoots
Sent: Thursday, 26 January 2012 3:21 AM
To: Histonet
Subject: [Histonet] Oil red O versus Sudan 4

Hi Histonetters
 
 
I am staining the plaques in aorta. I perfuse my animal with pbs before I 
biospy the aorta. I then pin the aorta down onto a wax plate and  then i stain 
the inside of the aorta for my plaques.
 
This seems to work well with the Sudan 4 but not so much with the Oil red o. 
The person who was doing the sudan 4 is no longer here and we have plenty of 
Oil red o. My thinking was that it should stain the same but I am not getting 
results with the Oil red o.
 
My question is what is the difference and what should I do differently with the 
Oil red o?
 
Thanks so much for your help!

I remain yours truely, 

Candice Camille 
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Re: [Histonet] Oil red O versus Sudan 4

[Candice Smoots]

Hi Sarah
 
 Actually, There is no micotmy involved. We biopsy the aorta. Clean the 
surrounding fat, cut it down the middle with a blade. And then we stain with 
Sudan 4. The aorta is mounted whole on a wax plate. we hydrate the tissue with 
PBS before we stain. This worked fine with the Sudan 4. Should I discontinue 
hydrating with PBS and use water instead with the Oil Red O.
 
Also with the Oil red O, we were rinsing them with isopranol before we stained 
them. Could this be the problem?

I remain yours truely, 

Candice Camille 



From: Sarah Dysart sdys...@mirnarx.com
To: Candice Smoots candice_cami...@yahoo.com 
Sent: Wednesday, January 25, 2012 10:32 AM
Subject: RE: [Histonet] Oil red O versus Sudan 4

I do oil red a lot.  You are doing it on frozen sections right?
What are you coverslipping with?
Need a few more details of the protocol and I might be able to help =)

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Candice Smoots
Sent: Wednesday, January 25, 2012 10:21 AM
To: Histonet
Subject: [Histonet] Oil red O versus Sudan 4

Hi Histonetters
 
 
I am staining the plaques in aorta. I perfuse my animal with pbs before I 
biospy the aorta. I then pin the aorta down onto a wax plate and  then i stain 
the inside of the aorta for my plaques.
 
This seems to work well with the Sudan 4 but not so much with the Oil red o. 
The person who was doing the sudan 4 is no longer here and we have plenty of 
Oil red o. My thinking was that it should stain the same but I am not getting 
results with the Oil red o.
 
My question is what is the difference and what should I do differently with the 
Oil red o?
 
Thanks so much for your help!

I remain yours truely, 

Candice Camille 
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Re: [Histonet] Oil red O versus Sudan 4

[Candice Smoots]

That would be great!
 
Thanks

I remain yours truely, 

Candice Camille 



From: Elizabeth Chlipala l...@premierlab.com
To: 'Candice Smoots' candice_cami...@yahoo.com; Histonet 
histonet@lists.utsouthwestern.edu 
Sent: Wednesday, January 25, 2012 10:43 AM
Subject: RE: [Histonet] Oil red O versus Sudan 4

Candice

Most of the references regarding this technique use Sudan IV, that's what we 
use here. We use Oil Red O for the Aortic Root Sections but not for the En Face 
Analysis of the Aorta.  I have the Sudan IV procedure we use here if you would 
like it.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Candice Smoots
Sent: Wednesday, January 25, 2012 9:21 AM
To: Histonet
Subject: [Histonet] Oil red O versus Sudan 4

Hi Histonetters


I am staining the plaques in aorta. I perfuse my animal with pbs before I 
biospy the aorta. I then pin the aorta down onto a wax plate and  then i stain 
the inside of the aorta for my plaques.

This seems to work well with the Sudan 4 but not so much with the Oil red o. 
The person who was doing the sudan 4 is no longer here and we have plenty of 
Oil red o. My thinking was that it should stain the same but I am not getting 
results with the Oil red o.

My question is what is the difference and what should I do differently with the 
Oil red o?

Thanks so much for your help!

I remain yours truely,

Candice Camille
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AW: [Histonet] Oil Red O for FS on Muscle

[Gudrun Lang]

Hi Akemi,
we don't do Oil Red O, but the Sudan III in an aceton-ethanol mixture. To
prevent the precipitates on the slide, we stain in a plastic coplin jar with
a screwed tap. The dye-precipitates accumulate on the ground. We take care
not to mix them up while putting in the slide. So we need no filtering
before staining. We only stain frozens of lung without fixation.

I don’t know the formula of Oil Red O solution, but perhaps it can be
handled like the sudan III.

bye
Gudrun

-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Akemi
Allison
Gesendet: Mittwoch, 26. Jänner 2011 18:16
An: histonet
Betreff: [Histonet] Oil Red O for FS on Muscle

Happy hump day!

Does anyone have a good procedure for Oil Red O for FS on Muscle.   
The  procedure the lab I am working with is having a great deal of  
problems with their muscle biopsy panel.  I am trouble-shooting some  
of the issues for the 1-Step Trichrome 3.4 pH, NADH, AT Pase,  I am  
working with them with pH issues.

The Oil Red O is very problematic.  They are mixing before use and  
filtering with 42 Watman paper, but there is a lot of residual  
background on the slides.  Also, they were not fixing the sections  
with 37% formaldehyde, even though the procedure calls for it.  Could  
you share who you get the stain from also.  Your assistance is  
greatly appreciated.

Akemi


Akemi Allison BS, HT (ASCP) HTL
Director
Phoenix Lab Consulting
Tele: 408.335.9994
E-Mail: akemiat3...@yahoo.com

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[Histonet] Oil Red O for FS on Muscle

[Michelle MacVeigh-Aloni]

Hi Akemi,

 

Order the stain from Electron Microscopy Sciences - cat # 26503-02.  It
comes as 250 ml solution and is a lot less expensive than some other
sources. I guess you can probably get it through VWR. 

 

Mix 3 parts of this stock with 2 parts dist. Water and let it stand for 10
min.

Filter through coarse filter paper. I have even used paper towel with no
problem. Anything finer will take forever to filter. 

Fix the sections with regular 10% NBF for few min. 3-5

Rinse slides in dist. Water few times

Working Oil -red-O for 10 min. I use the plastic containers for shipping
slides, because the stain will color the plastic and I can easily and
cheaply replace those, but you can wash them and reuse them multiple times.

70% alcohol - quick rinse to clear background

Dist. Water - 5 min

Mayers Hematoxylin 5 min

Tap water 10 min

Dist. water rinse or up to 1 min

Mount with Aqua Mount

 

Never had any problems. Normal muscle will have very, very tiny droplets. We
used it on rodent muscle, but it should work the same for human.

 

Good luck

Michelle Aloni 

 

Research Specialist

USC Keck School of Medicine

 

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[Histonet] Oil Red O for FS on Muscle

[Michelle MacVeigh-Aloni]

Few more things:

 

1.   The mixed/working solution is good for 1-2 hours only. If you
filter it for a long time, this might use the good working time and then you
have trouble, because it just won't work well for you. I just poor the stain
and the water in a glass cylinder and shake it. That's it. 

2.   After coverslipping, do not press on the coverslip to chase air
bubbles away or this might displace the lipid droplets from their original
place.

3.   Look at the slides as soon as possible. In few days, you will see
that the stain is changing into ugly black crystals and the longer you wait
the larger the crystals = have to repeat it all over again J

4.   To clean the glassware, spray All Purpose Cleaner, use a brush and
warm water

 

Michelle

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[Histonet] Oil Red O for FS on Muscle

[Akemi Allison]

Happy hump day!

Does anyone have a good procedure for Oil Red O for FS on Muscle.   
The  procedure the lab I am working with is having a great deal of  
problems with their muscle biopsy panel.  I am trouble-shooting some  
of the issues for the 1-Step Trichrome 3.4 pH, NADH, AT Pase,  I am  
working with them with pH issues.


The Oil Red O is very problematic.  They are mixing before use and  
filtering with 42 Watman paper, but there is a lot of residual  
background on the slides.  Also, they were not fixing the sections  
with 37% formaldehyde, even though the procedure calls for it.  Could  
you share who you get the stain from also.  Your assistance is  
greatly appreciated.


Akemi


Akemi Allison BS, HT (ASCP) HTL
Director
Phoenix Lab Consulting
Tele: 408.335.9994
E-Mail: akemiat3...@yahoo.com

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RE: [Histonet] Oil Red O for FS on Muscle

[Setlak, Lisa]

Hi,
We use Oil Red O - but we don't do it on muscle. Our protocol is we filter the 
whole bottle before we use it and then we apply it to unfixed slides for about 
30 minutes. Rinse in tap water, counterstain with Hematoxylin and coverslip 
with Aquamount. We use it for cytospins and occasionally on various frozen 
tissues. Our stain is from American Mastertech.
Hope this helps,
Lisa V.
Children's Memorial Hospital
Chicago, IL 60616
773-868-8949

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison
Sent: Wednesday, January 26, 2011 11:16 AM
To: histonet
Subject: [Histonet] Oil Red O for FS on Muscle

Happy hump day!

Does anyone have a good procedure for Oil Red O for FS on Muscle.   
The  procedure the lab I am working with is having a great deal of  
problems with their muscle biopsy panel.  I am trouble-shooting some  
of the issues for the 1-Step Trichrome 3.4 pH, NADH, AT Pase,  I am  
working with them with pH issues.

The Oil Red O is very problematic.  They are mixing before use and  
filtering with 42 Watman paper, but there is a lot of residual  
background on the slides.  Also, they were not fixing the sections  
with 37% formaldehyde, even though the procedure calls for it.  Could  
you share who you get the stain from also.  Your assistance is  
greatly appreciated.

Akemi


Akemi Allison BS, HT (ASCP) HTL
Director
Phoenix Lab Consulting
Tele: 408.335.9994
E-Mail: akemiat3...@yahoo.com

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RE: [Histonet] Oil Red O for FS on Muscle

[Liz Chlipala]

Allison

We make up our Oil Red O from scratch same day we use it, let it stand 10 
minutes, and then filter with a Millipore Stericup 0.22µm, GP Express Plus 
Membrane, 250ml Receiver Bottle, catalog number: SCGPU02RE under vacuum.  That 
seems to help a bit with the background and we are working with a really clear 
solution. We have not run muscles but aortic root and liver on mouse and rat.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison
Sent: Wednesday, January 26, 2011 10:16 AM
To: histonet
Subject: [Histonet] Oil Red O for FS on Muscle

Happy hump day!

Does anyone have a good procedure for Oil Red O for FS on Muscle.   
The  procedure the lab I am working with is having a great deal of  
problems with their muscle biopsy panel.  I am trouble-shooting some  
of the issues for the 1-Step Trichrome 3.4 pH, NADH, AT Pase,  I am  
working with them with pH issues.

The Oil Red O is very problematic.  They are mixing before use and  
filtering with 42 Watman paper, but there is a lot of residual  
background on the slides.  Also, they were not fixing the sections  
with 37% formaldehyde, even though the procedure calls for it.  Could  
you share who you get the stain from also.  Your assistance is  
greatly appreciated.

Akemi


Akemi Allison BS, HT (ASCP) HTL
Director
Phoenix Lab Consulting
Tele: 408.335.9994
E-Mail: akemiat3...@yahoo.com

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Re: [Histonet] Oil Red O for FS on Muscle

[Grantham, Andrea L - (algranth)]

I've done many of these stains and I get my ORO stain from Poly Scientific. 
Actually I use the whole kit and I use Freida's protocol in the second edition. 
That includes fixing in 37% formaldehyde. I filter the ORO just before use and 
usually get nice clean slides. If there is a residue it isn't much and wipes 
off easily. I even re-use the ORO stain once in a while and it still comes out 
great. My slides are often used for quantifying the amount of lipids so I don't 
counterstain most of the time.

Andi Grantham




On Jan 26, 2011, at 10:15 AM, Akemi Allison wrote:

 Happy hump day!
 
 Does anyone have a good procedure for Oil Red O for FS on Muscle.   
 The  procedure the lab I am working with is having a great deal of  
 problems with their muscle biopsy panel.  I am trouble-shooting some  
 of the issues for the 1-Step Trichrome 3.4 pH, NADH, AT Pase,  I am  
 working with them with pH issues.
 
 The Oil Red O is very problematic.  They are mixing before use and  
 filtering with 42 Watman paper, but there is a lot of residual  
 background on the slides.  Also, they were not fixing the sections  
 with 37% formaldehyde, even though the procedure calls for it.  Could  
 you share who you get the stain from also.  Your assistance is  
 greatly appreciated.
 
 Akemi
 
 
 Akemi Allison BS, HT (ASCP) HTL
 Director
 Phoenix Lab Consulting
 Tele: 408.335.9994
 E-Mail: akemiat3...@yahoo.com
 
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[Histonet] Oil Red O

[Chiriboga, Luis]

Hi Everyone
Posting this for a  colleague who is doing oil red o staining on carotid 
plaques and is seeing both large globular and abundant fine small  globular 
staining.  Localization is correct  but she is concerned about the amount and 
size variability of the staining pattern.  She has been using the propylene 
glycol method on frozen tissue and has tried a few variations but keeps getting 
the same pattern.  Can provide a picture if you  email me offline .  Any advice 
would be helpful.
Thanks
Luis
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Re: [Histonet] Oil Red O

[Jay Lundgren]

 That's the way it normally looks, kinda globby. ORO is really not that
soluble in fat, just more soluble than the prop. glycol.

Jay A. Lundgren M.S., HTL
(ASCP)
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[Histonet] Oil Red O trivia

[Finlay Finlay]

Hello
 
Just wondering if anyone knows the what the 'O' in oil red O stands for.
I suspect that it stands for nothing other than O similar to the 'G' in
Orange G. One of our pathologists is looking for a tricky stain question
to annoy trainees with.
 
Thanks for your help
 
Finlay
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Re: [Histonet] Oil Red O trivia

[Geoff McAuliffe]

So, the pathologist does not know the answer but he wants to annoy 
trainees? Glad I don't work for him!


Geoff

Finlay Finlay wrote:

Hello
 
Just wondering if anyone knows the what the 'O' in oil red O stands for.

I suspect that it stands for nothing other than O similar to the 'G' in
Orange G. One of our pathologists is looking for a tricky stain question
to annoy trainees with.
 
Thanks for your help
 
Finlay

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--
--
**
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcaul...@umdnj.edu

**



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[Histonet] Oil Red O control

[Inman, Anna]

Hello -

How does everyone handle having a positive control for Oil Red O - We
very infrequently have this stain and have had difficulty keeping
control on hand. 

 

 

Thank you 

Anna

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Re: [Histonet] Oil Red O control

[Drew Meyer]

There was a discussion on this recently, so you may want to search the
archives for it.  In my opinion, the easiest and best control to use is
Mayo.  Just smear it on a slide as you need it and there you go.  Cheap and
easy.

Drew

On Wed, Nov 10, 2010 at 09:45, Inman, Anna anna.in...@stmarygj.org wrote:

 Hello -

 How does everyone handle having a positive control for Oil Red O - We
 very infrequently have this stain and have had difficulty keeping
 control on hand.





 Thank you

 Anna

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 contact the sender by reply e-mail and destroy all copies of the original
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RE: [Histonet] Oil Red O control

[Sun,Yuping]

Hello Histonet Users,

I have a question about Oil Red O staining.
Could the Oil Red O solution(Poly Scientific, Cat.No. S1848) be reused?

I would appreciate any feedback whatsoever. 

Thank you very much.
Cathy 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Drew Meyer
Sent: Wednesday, November 10, 2010 9:55 AM
To: Inman, Anna
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Oil Red O control

There was a discussion on this recently, so you may want to search the
archives for it.  In my opinion, the easiest and best control to use is
Mayo.  Just smear it on a slide as you need it and there you go.  Cheap and
easy.

Drew

On Wed, Nov 10, 2010 at 09:45, Inman, Anna anna.in...@stmarygj.org wrote:

 Hello -

 How does everyone handle having a positive control for Oil Red O - We
 very infrequently have this stain and have had difficulty keeping
 control on hand.





 Thank you

 Anna

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RE: [Histonet] Oil Red O control

[Michelle MacVeigh-Aloni]

Once you mix your stock solution with water, it is good for only about an
hour. Then you have to discard it. 

 

Michelle Aloni MS HTL (ASCP)

USC Keck School of Medicine

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Re: [Histonet] Oil Red O control

[Lee Peggy Wenk]

Cut frozen section of adrenal from an autopsy or surgical pathology. Store 
slides in a box in the refrig. Good for a year.


Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073

--
From: Inman, Anna anna.in...@stmarygj.org
Sent: Wednesday, November 10, 2010 9:45 AM
To: Geoff McAuliffe mcaul...@umdnj.edu; Finlay Finlay 
f.fin...@formed.gla.ac.uk

Cc: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Oil Red O control


Hello -

How does everyone handle having a positive control for Oil Red O - We
very infrequently have this stain and have had difficulty keeping
control on hand.





Thank you

Anna

CONFIDENTIALITY NOTICE:  This e-mail message, including any attachments, 
is for the sole use of the intended recipient(s) and may contain 
confidential and privileged information. Any unauthorized review, use, 
disclosure or distribution is prohibited. If you are not the intended 
recipient, please contact the sender by reply e-mail and destroy all 
copies of the original message.

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[Histonet] Oil Red O Staining

[Willman, Sharon]

Hi,
Other than using plus slides and air drying, what other techniques do you use 
to help frozen section tissues for Oil Red O to adhere to the slides?  How long 
do you usually air dry slides for cryostat sectioning?
Thanks in advance for your input.
Sharon



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[Histonet] Oil Red O protocol

[Aprill Watanabe]

I¹m looking for a protocol for Oil Red O that does not use propylene glycol
to make the Oil Red O.  Does anyone have a protocol that I could use?

Aprill Watanabe, B.S.
Research Associate
Integrated Cancer Genomics Division
Tissue Microarray Center (TMA)
Translational Genomics Research Institute (TGen)
main: 602-343-8822
Fax: 602-343-8840
awatan...@tgen.org
www.tgen.org

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RE: [Histonet] Oil red o mounting media

[Ingles Claire]

We use Crystalmount. You don't even have to coverslip. Just make sure you 
evenly distribute the mountant on the slide and let it dry. We usually put it 
on a hot plate for a bit. This leaves a hardened shell around the tissue. The 
slides can be read like this too. If you really need a coverslip, it can be 
quick dipped in xylene and coverslipped with your regular mounting media. You 
can also coverslip directly with crystal mount, but we find it dries around the 
section and creates a big air bubble encompassing the tissue. This technique 
also has the added benefit of keeping the fat (and the stain) in the correct 
areas and is not squeezed out from the pressure during direct mounting. 
 
Claire


 

Hi,

I will be staining many samples for Oil red O in the near future.  I was
wondering if there were any aqueous mounting medias out there that
perform superior to Glycergel? 

Thanks

Dave




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Re: [Histonet] Oil Red O despair - point of clarification

[Andrea Grantham]

I didn't mean that the propylene glycol and glycerin jelly were 
combined - just wanted to clear that up. The protocol uses both but 
for different things.


Andi Grantham



At 09:12 AM 11/18/2008, Andrea Grantham wrote:
I get many projects here that require frozen liver sections and Oil 
Red O staining. Some years ago I was having problems with the 
staining and someone on histonet suggested the PolyScientific R  D 
Oil Red O Stain Kit. I got the kit and have been using it ever 
since. The stain works great - usually I have livers loaded with 
lipid - and the slides come out clean. The kit uses proplyene glycol 
and glycerin jelly to mount the coverslips. I do use one part of the 
ORO protocol from Freida Carson's book - I fix the slides in 37-40% 
(page 152, second edition).


Andi Grantham




At 04:49 PM 11/17/2008, Amos Brooks wrote:

Michele,
   You could try 0.5% Oil Red O in 60% triethylphosphate. That works really
well here. I've also used a formulation I found in Humanson, the same amount
of Oil Red O in 99% ethanol and it worked fairly well. Triethylphosphate is
much cleaner than the ethanol. (There was a precipitate on the slide.) I
can't avouch for isopropyl as I used ethanol, but it is good to know there's
an alternative in case we run out of triethylphosphate.

Amos Brooks

Message: 10
Date: Mon, 17 Nov 2008 11:56:45 -0600
From: Michele Wich [EMAIL PROTECTED]
Subject: [Histonet] Oil Red O despair
To: histonet@lists.utsouthwestern.edu
Message-ID:
   [EMAIL PROTECTED]
Content-Type: text/plain;   charset=us-ascii

I'm wondering what are the pros (or cons) of using the propylene glycol
version of Oil Red O over the isopropanol method. Is one more suited to
a specific application?

I'm trying to stain a frozen section of liver, which one would suspect
would be loaded with fat, and have thus far been unsuccessful using the
propylene glycol Oil Red O.

Is there something obvious that I'm missing here? I'm new to
cryosectioning...perhaps I did something wrong in the cryostat. I fixed
my sections in NBF before staining.

Please help. I'm feeling like a pathetic excuse for a histotech. Any
advice is greatly appreciated.
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.
: Andrea Grantham, HT(ASCP) Dept. of Cell Biology  Anatomy :
: Sr. Research Specialist   University of Arizona   :
: (office:  AHSC 4212)  P.O. Box 245044 :
: (voice:  520-626-4415)Tucson, AZ  85724-5044USA   :
: (FAX:  520-626-2097)  (email:  [EMAIL PROTECTED])   :
:...:
  http://www.cba.arizona.edu/histology-lab.html


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.
: Andrea Grantham, HT(ASCP) Dept. of Cell Biology  Anatomy :
: Sr. Research Specialist   University of Arizona   :
: (office:  AHSC 4212)  P.O. Box 245044 :
: (voice:  520-626-4415)Tucson, AZ  85724-5044USA   :
: (FAX:  520-626-2097)  (email:  [EMAIL PROTECTED])   :
:...:
  http://www.cba.arizona.edu/histology-lab.html


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[Histonet] Oil Red O despair

[Amos Brooks]

Michele,
   You could try 0.5% Oil Red O in 60% triethylphosphate. That works really
well here. I've also used a formulation I found in Humanson, the same amount
of Oil Red O in 99% ethanol and it worked fairly well. Triethylphosphate is
much cleaner than the ethanol. (There was a precipitate on the slide.) I
can't avouch for isopropyl as I used ethanol, but it is good to know there's
an alternative in case we run out of triethylphosphate.

Amos Brooks

Message: 10
Date: Mon, 17 Nov 2008 11:56:45 -0600
From: Michele Wich [EMAIL PROTECTED]
Subject: [Histonet] Oil Red O despair
To: histonet@lists.utsouthwestern.edu
Message-ID:
   [EMAIL PROTECTED]
Content-Type: text/plain;   charset=us-ascii

I'm wondering what are the pros (or cons) of using the propylene glycol
version of Oil Red O over the isopropanol method. Is one more suited to
a specific application?

I'm trying to stain a frozen section of liver, which one would suspect
would be loaded with fat, and have thus far been unsuccessful using the
propylene glycol Oil Red O.

Is there something obvious that I'm missing here? I'm new to
cryosectioning...perhaps I did something wrong in the cryostat. I fixed
my sections in NBF before staining.

Please help. I'm feeling like a pathetic excuse for a histotech. Any
advice is greatly appreciated.
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