Re: [Histonet] Xylene substitutes for clearing agents

[Rene J Buesa via Histonet]

 Under separate cover I am sending you 3 papers of mine that answer your 
question.René
On Wednesday, August 28, 2019, 08:45:31 AM EDT, Ingles Claire via Histonet 
 wrote:  
 
 Chris:
Propar from Anatech works great for us. I believe it is still advisable to use 
xylene in the cleaning cycle on the processors though. We had to go back the 
other way a bit when our Doc wanted a tape coverslipper. Now he gripes about 
the xylene smell. Hmmm.
Claire


From: Hagon, Christopher (Health) via Histonet 

Sent: Tuesday, August 27, 2019 10:53 PM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Xylene substitutes for clearing agents

WARNING: This email appears to have originated outside of the UW Health email 
system.
DO NOT CLICK on links or attachments unless you recognize the sender and know 
the content is safe.




UNOFFICIAL

Hello histonetters!

I realise that this has been asked a lot, but cannot find a good link for the 
comparisons of each. I am charged with looking into converting our lab to go 
xylene free. We don't want to go down the limonene path, so that leaves the 
isopropyl alcohol method, or the aliphatic hydrocarbon xylene substitution 
(Leica Sub-X etc).

Looking for opinions from each camp if possible, on how easy or hard it was to 
change procedures. Was there much trial and error in changing the processing 
protocols with the aliphatics? Any pitfalls I should look out for?

Any input greatly appreciated.


Chris Hagon | Senior Scientist, Anatomical Pathology
ACT Pathology | health.act.gov.au<https://health.act.gov.au/>



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Re: [Histonet] Xylene substitutes for clearing agents

[Ingles Claire via Histonet]

Chris:
Propar from Anatech works great for us. I believe it is still advisable to use 
xylene in the cleaning cycle on the processors though. We had to go back the 
other way a bit when our Doc wanted a tape coverslipper. Now he gripes about 
the xylene smell. Hmmm.
Claire


From: Hagon, Christopher (Health) via Histonet 

Sent: Tuesday, August 27, 2019 10:53 PM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Xylene substitutes for clearing agents

WARNING: This email appears to have originated outside of the UW Health email 
system.
DO NOT CLICK on links or attachments unless you recognize the sender and know 
the content is safe.




UNOFFICIAL

Hello histonetters!

I realise that this has been asked a lot, but cannot find a good link for the 
comparisons of each. I am charged with looking into converting our lab to go 
xylene free. We don't want to go down the limonene path, so that leaves the 
isopropyl alcohol method, or the aliphatic hydrocarbon xylene substitution 
(Leica Sub-X etc).

Looking for opinions from each camp if possible, on how easy or hard it was to 
change procedures. Was there much trial and error in changing the processing 
protocols with the aliphatics? Any pitfalls I should look out for?

Any input greatly appreciated.


Chris Hagon | Senior Scientist, Anatomical Pathology
ACT Pathology | health.act.gov.au<https://health.act.gov.au/>



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[Histonet] Xylene substitutes for clearing agents

[Hagon, Christopher (Health) via Histonet]

UNOFFICIAL

Hello histonetters!

I realise that this has been asked a lot, but cannot find a good link for the 
comparisons of each. I am charged with looking into converting our lab to go 
xylene free. We don't want to go down the limonene path, so that leaves the 
isopropyl alcohol method, or the aliphatic hydrocarbon xylene substitution 
(Leica Sub-X etc).

Looking for opinions from each camp if possible, on how easy or hard it was to 
change procedures. Was there much trial and error in changing the processing 
protocols with the aliphatics? Any pitfalls I should look out for?

Any input greatly appreciated.


Chris Hagon | Senior Scientist, Anatomical Pathology
ACT Pathology | health.act.gov.au



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Re: [Histonet] Xylene substitutes in staining line

[Gayle Callis]

You wrote: 

 

Hello Histo Land,

Does anyone use xylene substitutes in their stain line only? I am especially
interested in d-limonene options. Thanks in advance!

 


*

 

We used xylene substitutes in the stain line for both deparaffinization and
dehydration but did not use limonene (citrus smelling).   We preferred the
Clearite 3 (Richard Allan) i.e. single aliphatic hydrocarbons  also
available from other vendors, or Propar (Anatech)  for this purpose.
Limonene was particularly offensive and personally made me turn green with
nausea!  It was banned from my lab due to intolerance to the smell which
also included banning limonene based household cleaners in my home.I
think people can become sensitized to this substitute so be careful about
handling and working under a hood.   We also used Clearite 3 for tissue
processing to eliminate xylene.   

 

There are caveats about using xylene substitutes.  They are sensitive to
residual water carry over and do not clear water as well as xylene.  We
added an extra stations to deparaffinize sections and at the end of
dehydration sequence ensure NO residual water carry over before mounting a
cover glass.  On some high humidity days, Clearite 3 would be cloudy meaning
the last stations with Clearite had to be changed to fresh solvent not
exposed to humid air.  To combat a water carryover problem commonly not seen
with xylene and its ability to handle water carryover better, we rotated the
substitutes frequently during a work week so the last station was fresh.
If you have cloudy looking sections after cover slipping from a xylene
substitute, then you have water carry over.  A hint is if the last alcohol
in the dehydration series is pink, then there is water carryover.   Due
diligence is necessary to avoid poor paraffin removal and also good
dehydration.   In a very busy laboratory, this means extra time and expense
to deal with a xylene substitute.

 

The test for paraffin carry over in  deparaffinization sequence, one can
pipette a few mls of last 95% alcohol (closest to water) into a glass beaker
of tap water.  It the aliquot turns the water cloudy, then you have paraffin
carry over into the alcohol.  If not seen in last 95%, the test the 95%
station by working backwards in the deparaffinizing sequence.  If any
alcohol test is cloudy, then that alcohol and all previous stations were
changed in our lab.  Some rotation of clear test was done into an earlier
station slot. This will test also works when using xylene.  


However, I really like the emerging practice of using hot soapy water for
deparaffinizing sections recently discussed in depth on Histonet.   There is
a wonderful publication by Tony Henwood et al,  J of Histotechnol 2013;
36(2):45-50.  Tresa Goins also entered in on this discussion about their
lab's success with this method.  Go to Histonet Archives and read the
commentary.   It certainly means less dependence on organic solvents, less
exposure to potentially toxic chemicals, and the expense of solvent
disposal.   I think the soap method is definitely worth trying. 


Hope this helps


Gayle M. Callis  HTL/HT/MT(ASCP)  


 

 

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[Histonet] Xylene Substitutes

[Alicia Lange]

Hello Histo Land,
Does anyone use xylene substitutes in their stain line only? I am
especially interested in d-limonene options. Thanks in advance!
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Re: [Histonet] Xylene Substitutes

[Rene J Buesa]

We used NOTHING in our staining line. We dewaxed with 2% dishwasher soap 
followed by the normal staining (no xylene → no ethanol).After staining we 
dried the stained section in an oven at 60ºC for 15 minutes → coverslip (no 
ethanol → no xylene).To process we used iso-propanol → mineral oil → paraffin 
wax no ethanol → no xylene).Try it, you will love it.René 


 On Wednesday, June 24, 2015 1:03 PM, Alicia Lange 
aliciarosela...@gmail.com wrote:
   

 Hello Histo Land,
Does anyone use xylene substitutes in their stain line only? I am
especially interested in d-limonene options. Thanks in advance!
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[Histonet] Xylene Substitutes

[Yaskovich, Ruth A (NIH/NIDCR) [E]]

I know this question has been asked before but what's the best and safest 
Xylene Substitute?
Ruth Yaskovich
N.I.H. N.I.D.C.R.
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Re: [Histonet] Xylene Substitutes

[Rene J Buesa]

Ruth:
I all reality none is good. Both alkanes or D-Limonene are either as dangerous 
or more than xylene, and all perform below the xylene standard.
Any one requires modifications of all procedures, they seldom dewax adequately 
and all constitute a processing compromise. Many cannot de recycled.
Please go to http://www.histosearch.com/rene.html and read about xylene 
substitution.
 
The optimal solution to eliminate xylene consists on:
1- dehydrate with 2-propanol (or pure isopropyl alcohol or IPA)
2- do not use any ante-medium and go from IPA directly to paraffin wax or 
ideally to mineral oil:IPA at 1:1
3- infiltrate as usual
4- dewax sections with 2%aq. sol. of dishwater soap at 90ºC for 1 min twice
5- stain as usual
6- dehydrate stained sections in a convection oven at 60ºC for 5 minutes
7- coverslip as usual.
As you can see xylene is nowhere to be found in this sequence that produce 
optimal quality slides.
Try it!
René J.



From: Yaskovich, Ruth A (NIH/NIDCR) [E] ryaskov...@dir.nidcr.nih.gov
To: 'Histonet@lists.utsouthwestern.edu' Histonet@lists.utsouthwestern.edu 
Sent: Wednesday, August 21, 2013 11:18 AM
Subject: [Histonet] Xylene Substitutes


I know this question has been asked before but what's the best and safest 
Xylene Substitute?
Ruth Yaskovich
N.I.H. N.I.D.C.R.
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RE: [Histonet] Xylene Substitutes

[Dingersoll]

   Donna S. Ing= ersoll, B.S., HTL, CT(ASCP)

   Laboratory Manager

   A P Laboratories, LLC
   2090 Executive Hall Rd S= uite 165
   Charleston= , SC 29407
   843-300-= 3001 X 202
   843-300-= 3003 (fax)
   [1]dingers...@aplaboratories.com   The  contents  of  this  message, 
together = with any attachments, are
   intended  only  for  the  use  of  the  person(s)  to whi= ch they are
   addressed  and  may  contain  confidential  and/or  privileged inform   
ation.  Further,  any  medical  information herein is confidential and
   protecte=  d  by  law. It is unlawful for unauthorized persons to use,
   review,   copy,   dis=  close,  or  disseminate  confidential  medical
   information.  If  you  are  not  the = intended recipient, immediately
   advise  the  sender and delete this message a= nd any attachments. Any
   distribution,  or  copying  of  this  message, or any at= tachment, is
   prohibited.

   



   
 Original M= essage 
   
Subject: Re: [Histonet] Xylene Substitutes
   
From= : Rene J Buesa [2]rjbu...@yahoo.com
   
Date: Wed, August 21, 2013 12:06 pm
   
To:Yaskovich,Ruth=A(NIH/NIDCR)[E][3]rya   
skov...@dir.nidcr.nih.gov,
   
'[4]Histonet@lists.utsouthwestern.edu'
   
Histonet@lists.utsouthwest= ern.edu
   

   
Ruth:
   
I all reality none is good. B= oth alkanes or D-Limonene are either
   as  dangerous  or more than xylene, and = all perform below the xylene
   standard.
   
Any  one  requires  modifications=  of  all procedures, they seldom
   dewax  adequately and all constitute a proce= ssing compromise. Many
   cannot de recycled.
   
Please go to [5]http://www.histosearch.com/rene.html= /a and read
   about xylene substitution.
   

   
The optimal solu= tion to eliminate xylene consists on:
   
1- dehydrate with 2-propanol (o= r pure isopropyl alcohol or IPA)
   
2-  do  not  use  any  ante-medium  and  =  go from IPA directly to
   paraffin wax or ideally to mineral oil:IPA at = 1:1
   
3- infiltrate as usual
   
4-  dewax  sections with 2%aq. sol. of= dishwater soap at 90ºC
   for 1 min twice
   
5- stain as usual
   
=  6-  dehydrate stained sections in a convection oven at 60ºC
   for 5 minut= es
   
7- coverslip as usual.
   
As  you  can  see  xylene is nowhere to b= e found in this sequence
   that produce optimal quality slides.
   
Try it!   
René J.
   

   

   

   
From: Yaskovich, Ruth A (NIH/NIDCR) [E]
   [6]ryaskov...@dir.nidcr.nih.gov
   
To: '[7]Histonet@lists.utsouthwes= tern.edu' [8]His   
to...@lists.utsouthwestern.edu
   
Sent: Wednesday, August 21, 2= 013 11:18 AM
   
Subject: [Histonet] Xylene Substitutes
   

   

   =  
I  know  this question has been asked before but what's the best
   and safes= t Xylene Substitute?
   
Ruth Yaskovich
   
N.I.H. N.I.D.C.R.
   
___= 
   
Histonet mailing list
   
[9]histo...@lists.uts= outhwestern.edu
   
[10]http://lists.utsouthwestern.edu/mailman/listinfo/histo= net
   
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Histone= t mailing list
   
[11]H= isto...@lists.utsouthwestern.edu
   
[12]http://lists.utsouthwestern.edu/mailm= an/listinfo/histonet
   



   


References

   1. =mailto:dingers...@aplaboratories.com;
   2. 3Dmailto:rjbu...@yahoo.com;
   3. 3Dmailto:ryaskov...@dir.nidcr.nih.gov;
   4. 3Dmailto:Histonet@lists   5. file://localhost/tmp/3D   6. 
3Dmailto:ryaskov   7. 3Dmailto:Histonet@lists.utsouthwestern.edu;
   8. 3Dmailto:Histonet@lists.utsouthwestern.edu;
   9. 3Dmailto:Histonet@lists.utsouthwestern.edu;
  10. 3Dhttp://lists.utsouthwestern.edu/mailma  11. 
3Dmailto:Histonet@lists.utsouthwestern.edu;
  12. 3Dhttp://lists.utsouthw=/
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Re: [Histonet] Xylene Substitutes

[Rene J Buesa]

Donna:
Please go to the web site I indicated in my previous answer and you find 
detailed answer.
After you stain the sections (regardless of the procedure) you dry them in an 
oven at 60ºC for 5 mins. and they are completely dry (ANATHEMA!!! to most) 
you just place them in your automated coverslipper with the same mounting 
medium you are using now. You need to change NOTHING.
Try it!
René J.



From: dingers...@aplaboratories.com dingers...@aplaboratories.com
To: Rene J Buesa rjbu...@yahoo.com; Yaskovich, Ruth A (NIH/NIDCR) [E] 
ryaskov...@dir.nidcr.nih.gov; 'Histonet@lists.utsouthwestern.edu' 
Histonet@lists.utsouthwestern.edu 
Sent: Wednesday, August 21, 2013 12:20 PM
Subject: RE: [Histonet] Xylene Substitutes



Rene,

How do you recommend cover slipping with out xylene?  We use a tape cover slip 
instrument that requires xylene.  If you are using glass coverslips what kind 
of adhesive is used?  I am very interested in eliminating xylene as much as 
possible, but we are a high volume lab and many of the automated instruments 
(stainers, coverslippers) require xylene.  Thanks in advance for your feedback.

Donna S. Ingersoll, B.S., HTL, CT(ASCP)
Laboratory Manager
A P Laboratories, LLC
2090 Executive Hall Rd Suite 165
Charleston, SC 29407
843-300-3001 X 202
843-300-3003 (fax)
dingers...@aplaboratories.com

The contents of this message, together with any attachments, are intended only 
for the use of the person(s) to which they are addressed and may contain 
confidential and/or privileged information. Further, any medical information 
herein is confidential and protected by law. It is unlawful for unauthorized 
persons to use, review, copy, disclose, or disseminate confidential medical 
information. If you are not the intended recipient, immediately advise the 
sender and delete this message and any attachments. Any distribution, or 
copying of this message, or any attachment, is prohibited.


 Original Message 
Subject: Re: [Histonet] Xylene Substitutes
From: Rene J Buesa rjbu...@yahoo.com
Date: Wed, August 21, 2013 12:06 pm
To: Yaskovich, Ruth A (NIH/NIDCR) [E] ryaskov...@dir.nidcr.nih.gov,
'Histonet@lists.utsouthwestern.edu'
Histonet@lists.utsouthwestern.edu

Ruth:
I all reality none is good. Both alkanes or D-Limonene are either as dangerous 
or more than xylene, and all perform below the xylene standard.
Any one requires modifications of all procedures, they seldom dewax adequately 
and all constitute a processing compromise. Many cannot de recycled.
Please go to http://www.histosearch.com/rene.html and read about xylene 
substitution.
 
The optimal solution to eliminate xylene consists on:
1- dehydrate with 2-propanol (or pure isopropyl alcohol or IPA)
2- do not use any ante-medium and go from IPA directly to paraffin wax or 
ideally to mineral oil:IPA at 1:1
3- infiltrate as usual
4- dewax sections with 2%aq. sol. of dishwater soap at 90ºC for 1 min twice
5- stain as usual
6- dehydrate stained sections in a convection oven at 60ºC for 5 minutes
7- coverslip as usual.
As you can see xylene is nowhere to be found in this sequence that produce 
optimal quality slides.
Try it!
René J.



From: Yaskovich, Ruth A (NIH/NIDCR) [E] ryaskov...@dir.nidcr.nih.gov
To: 'Histonet@lists.utsouthwestern.edu' Histonet@lists.utsouthwestern.edu 
Sent: Wednesday, August 21, 2013 11:18 AM
Subject: [Histonet] Xylene Substitutes


I know this question has been asked before but what's the best and safest 
Xylene Substitute?
Ruth Yaskovich
N.I.H. N.I.D.C.R.
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RE: [Histonet] Xylene substitutes for tissue processor clean cycle

[Boyd, Debbie M]

We have two Excelsiors (had Three).  We use Americlear in the cleaning cycle.  
We have never had issues with our cleaning cycle or the operation of our 
processors.  Our oldest processor was 12 years old before the oven cracked and 
started leaking.  The repair was too expensive for an older processor so we 
replaced it with a new one.  Americlear can only be purchased through 
Allegiance (Baxter).


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vanessa Avalos
Sent: Tuesday, May 21, 2013 8:01 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Xylene substitutes for tissue processor clean cycle

I use a excelsior and was told to always use Xylene in the cleaning cycle.  
Since sub is not as pure as xylene ( obviously) you could be at risk of 
developing paraffin clogs that the sub may not get. Using one xylene  in the 
cleaning has not effected the odor at all in the lab. Good Luck.

V.Avalos
ADS, INC
Fax:602-277-2134


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Conway, Carla
Sent: Tuesday, May 21, 2013 8:26 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Xylene substitutes for tissue processor clean cycle

Hello all,

The technician who does preventive maintenance on our LX-120 tissue processor 
recommended that we use xylene as the solvent in our clean cycle.
We are considering using a xylene substitue in the clean cycle and would 
appreciate any recommendations.

Thanks very much,


Carla Conway
Histology Technician
Western Fisheries Research Center, USGS
6505 N.E. 65th Street
Seattle, WA 98115-5016 USA
Phone: 206-526-6282 ext. 242
Fax: 206-526-6654
E-mail: cmcon...@usgs.gov
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[Histonet] Xylene substitutes for tissue processor clean cycle

[Conway, Carla]

Hello all,

The technician who does preventive maintenance on our LX-120 tissue
processor recommended that we use xylene as the solvent in our clean cycle.
We are considering using a xylene substitue in the clean cycle and would
appreciate any recommendations.

Thanks very much,


Carla Conway
Histology Technician
Western Fisheries Research Center, USGS
6505 N.E. 65th Street
Seattle, WA 98115-5016 USA
Phone: 206-526-6282 ext. 242
Fax: 206-526-6654
E-mail: cmcon...@usgs.gov
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Re: [Histonet] Xylene substitutes for tissue processor clean cycle

[Rene J Buesa]

Use a 5% solution of a strong lab detergent in isopropyl alcohol. Since the 
cleaning cycle is completed at 50ºC that will be enough and you will not have 
to expose yourself to xylene vapors.
René J.

From: Conway, Carla cmcon...@usgs.gov
To: histonet@lists.utsouthwestern.edu 
Sent: Tuesday, May 21, 2013 11:25 AM
Subject: [Histonet] Xylene substitutes for tissue processor clean cycle


Hello all,

The technician who does preventive maintenance on our LX-120 tissue
processor recommended that we use xylene as the solvent in our clean cycle.
We are considering using a xylene substitue in the clean cycle and would
appreciate any recommendations.

Thanks very much,


Carla Conway
Histology Technician
Western Fisheries Research Center, USGS
6505 N.E. 65th Street
Seattle, WA 98115-5016 USA
Phone: 206-526-6282 ext. 242
Fax: 206-526-6654
E-mail: cmcon...@usgs.gov
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RE: [Histonet] Xylene substitutes for tissue processor clean cycle

[Elizabeth Chlipala]

Carla

I think most tissue processor vendors recommend xylene for cleaning and do not 
recommend a xylene substitute.  This may have changed recently but I would 
suspect it would void your warranty if you were still under one if the vendor 
recommends xylene and you are using a xylene substitute for cleaning.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Laboratory Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
Work (303) 682-3949
Fax (303) 682-9060
Cell (303) 881-0763
l...@premierlab.com
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Conway, Carla
Sent: Tuesday, May 21, 2013 9:26 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Xylene substitutes for tissue processor clean cycle

Hello all,

The technician who does preventive maintenance on our LX-120 tissue processor 
recommended that we use xylene as the solvent in our clean cycle.
We are considering using a xylene substitue in the clean cycle and would 
appreciate any recommendations.

Thanks very much,


Carla Conway
Histology Technician
Western Fisheries Research Center, USGS
6505 N.E. 65th Street
Seattle, WA 98115-5016 USA
Phone: 206-526-6282 ext. 242
Fax: 206-526-6654
E-mail: cmcon...@usgs.gov
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RE: [Histonet] Xylene substitutes for tissue processor clean cycle

[Blazek, Linda]

Carla, 
We use Formula 83 in the clean cycle.  We do change it more often than you 
would xylene though.  

Linda

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Conway, Carla
Sent: Tuesday, May 21, 2013 11:26 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Xylene substitutes for tissue processor clean cycle

Hello all,

The technician who does preventive maintenance on our LX-120 tissue processor 
recommended that we use xylene as the solvent in our clean cycle.
We are considering using a xylene substitue in the clean cycle and would 
appreciate any recommendations.

Thanks very much,


Carla Conway
Histology Technician
Western Fisheries Research Center, USGS
6505 N.E. 65th Street
Seattle, WA 98115-5016 USA
Phone: 206-526-6282 ext. 242
Fax: 206-526-6654
E-mail: cmcon...@usgs.gov
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RE: [Histonet] Xylene substitutes for tissue processor clean cycle

[Tom McNemar]

We use Americlear.  Have not used Xylene for many, many years.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Conway, Carla
Sent: Tuesday, May 21, 2013 11:26 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Xylene substitutes for tissue processor clean cycle

Hello all,

The technician who does preventive maintenance on our LX-120 tissue
processor recommended that we use xylene as the solvent in our clean cycle.
We are considering using a xylene substitue in the clean cycle and would
appreciate any recommendations.

Thanks very much,


Carla Conway
Histology Technician
Western Fisheries Research Center, USGS
6505 N.E. 65th Street
Seattle, WA 98115-5016 USA
Phone: 206-526-6282 ext. 242
Fax: 206-526-6654
E-mail: cmcon...@usgs.gov
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RE: [Histonet] Xylene substitutes for tissue processor clean cycle

[Vanessa Avalos]

I use a excelsior and was told to always use Xylene in the cleaning cycle.  
Since sub is not as pure as xylene ( obviously) you could be at risk of 
developing paraffin clogs that the sub may not get. Using one xylene  in the 
cleaning has not effected the odor at all in the lab. Good Luck.

V.Avalos
ADS, INC
Fax:602-277-2134


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Conway, Carla
Sent: Tuesday, May 21, 2013 8:26 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Xylene substitutes for tissue processor clean cycle

Hello all,

The technician who does preventive maintenance on our LX-120 tissue processor 
recommended that we use xylene as the solvent in our clean cycle.
We are considering using a xylene substitue in the clean cycle and would 
appreciate any recommendations.

Thanks very much,


Carla Conway
Histology Technician
Western Fisheries Research Center, USGS
6505 N.E. 65th Street
Seattle, WA 98115-5016 USA
Phone: 206-526-6282 ext. 242
Fax: 206-526-6654
E-mail: cmcon...@usgs.gov
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RE: [Histonet] Xylene Substitutes

[Tom McNemar]

Not familiar with SubX but we have used Americlear for many years with good 
results.  You just can't beat Xylene for some things though and still keep a 
little around.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne 
Anderson
Sent: Tuesday, February 05, 2013 4:58 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Xylene Substitutes

Hello all,

My lab is looking into xylene substitutes, and I'd love some feedback on what 
other labs are using. We currently use SubX, but are there other items out 
there more economical?

Thanks,
Adrienne
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This e-mail, including attachments, is intended for the sole use of the 
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RE: [Histonet] Xylene Substitutes

[SimmsColon, Janine]

We use Pro Par Clearant through Fisher from Anatech. Health-1,
Flammablity-2, Instability-0. For a case of 4 gallons it runs us about
$170.

Janine Simms Colon, CPhT, HT(ASCP), BHA
Histology/Pathology
Johnson Memorial Hospital
201 Chestnut Hill Road
Stafford Springs, CT 06076
Office: 860-684-8230 ext. 5197
janine.simmsco...@jmmc.com

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne
Anderson
Sent: Tuesday, February 05, 2013 4:58 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Xylene Substitutes

Hello all,

My lab is looking into xylene substitutes, and I'd love some feedback on
what other labs are using. We currently use SubX, but are there other
items out there more economical?

Thanks,
Adrienne
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Re: [Histonet] Xylene Substitutes

[Adrienne Anderson]

Thanks so much, everyone, for the feedback. 

I have a colleague who is convinced paint thinner is exactly the same as the 
SubX we use. Does anyone know if this is true?

Thanks again,
Adrienne


On Feb 6, 2013, at 5:36 AM, Tom McNemar wrote:

 Not familiar with SubX but we have used Americlear for many years with good 
 results.  You just can't beat Xylene for some things though and still keep a 
 little around.
 
 Tom McNemar, HT(ASCP)
 Histology Co-ordinator
 Licking Memorial Health Systems
 (740) 348-4163
 (740) 348-4166
 tmcne...@lmhealth.org
 www.LMHealth.org
 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne 
 Anderson
 Sent: Tuesday, February 05, 2013 4:58 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Xylene Substitutes
 
 Hello all,
 
 My lab is looking into xylene substitutes, and I'd love some feedback on what 
 other labs are using. We currently use SubX, but are there other items out 
 there more economical?
 
 Thanks,
 Adrienne
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 This e-mail, including attachments, is intended for the sole use of the 
 individual and/or entity to whom it is addressed, and contains information 
 from Licking Memorial Health Systems which is confidential or privileged. If 
 you are not the intended recipient, nor authorized to receive for the 
 intended recipient, be aware that any disclosure, copying, distribution or 
 use of the contents of this e-mail and attachments is prohibited. If you have 
 received this in error, please advise the sender by reply e-mail and delete 
 the message immediately. You may also contact the LMH Process Improvement 
 Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be 
 secure or error-free as information could be intercepted, corrupted, lost, 
 destroyed, arrive late or incomplete, or contain viruses. The sender 
 therefore does not accept liability for any errors or omissions in the 
 contents of this message, which arise as a result of e-mail transmission. 
 Thank you.


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RE: [Histonet] Xylene Substitutes

[Vanessa Avalos]

I use XS-3 from Statlab for staining. Since it is substitute I had to do some 
adjusting to the stain line but once I got it right I have had no problems  We 
pay about $70 for 4 gal. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne 
Anderson
Sent: Wednesday, February 06, 2013 6:30 AM
To: Tom McNemar
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Xylene Substitutes

Thanks so much, everyone, for the feedback. 

I have a colleague who is convinced paint thinner is exactly the same as the 
SubX we use. Does anyone know if this is true?

Thanks again,
Adrienne


On Feb 6, 2013, at 5:36 AM, Tom McNemar wrote:

 Not familiar with SubX but we have used Americlear for many years with good 
 results.  You just can't beat Xylene for some things though and still keep a 
 little around.
 
 Tom McNemar, HT(ASCP)
 Histology Co-ordinator
 Licking Memorial Health Systems
 (740) 348-4163
 (740) 348-4166
 tmcne...@lmhealth.org
 www.LMHealth.org
 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne 
 Anderson
 Sent: Tuesday, February 05, 2013 4:58 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Xylene Substitutes
 
 Hello all,
 
 My lab is looking into xylene substitutes, and I'd love some feedback on what 
 other labs are using. We currently use SubX, but are there other items out 
 there more economical?
 
 Thanks,
 Adrienne
 ___
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 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 
 This e-mail, including attachments, is intended for the sole use of the 
 individual and/or entity to whom it is addressed, and contains information 
 from Licking Memorial Health Systems which is confidential or privileged. If 
 you are not the intended recipient, nor authorized to receive for the 
 intended recipient, be aware that any disclosure, copying, distribution or 
 use of the contents of this e-mail and attachments is prohibited. If you have 
 received this in error, please advise the sender by reply e-mail and delete 
 the message immediately. You may also contact the LMH Process Improvement 
 Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be 
 secure or error-free as information could be intercepted, corrupted, lost, 
 destroyed, arrive late or incomplete, or contain viruses. The sender 
 therefore does not accept liability for any errors or omissions in the 
 contents of this message, which arise as a result of e-mail transmission. 
 Thank you.


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Re: [Histonet] Xylene Substitutes

[Rene J Buesa]

The best solution to eliminate xylene is to use isopropyl alcohol mixed with 
mineral oil.
Xylene can be eliminated from staining by dewaxing with 2% aq. sol. of 
dishwasher soap.
Before coverslipping oven dry the stained sections and cover directly.
To clean tissue processors use a 2% mixture of strong lab lab-ware detergent.
Do all of the above and your lab will be xylene free.
René J.

From: Tom McNemar tmcne...@lmhealth.org
To: 'Adrienne Anderson' rennie1...@yahoo.com; 
histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu 
Sent: Wednesday, February 6, 2013 5:36 AM
Subject: RE: [Histonet] Xylene Substitutes

Not familiar with SubX but we have used Americlear for many years with good 
results.  You just can't beat Xylene for some things though and still keep a 
little around.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
http://www.lmhealth.org/


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne 
Anderson
Sent: Tuesday, February 05, 2013 4:58 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Xylene Substitutes

Hello all,

My lab is looking into xylene substitutes, and I'd love some feedback on what 
other labs are using. We currently use SubX, but are there other items out 
there more economical?

Thanks,
Adrienne
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individual and/or entity to whom it is addressed, and contains information from 
Licking Memorial Health Systems which is confidential or privileged. If you are 
not the intended recipient, nor authorized to receive for the intended 
recipient, be aware that any disclosure, copying, distribution or use of the 
contents of this e-mail and attachments is prohibited. If you have received 
this in error, please advise the sender by reply e-mail and delete the message 
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error-free as information could be intercepted, corrupted, lost, destroyed, 
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Re: [Histonet] Xylene Substitutes

[Rene J Buesa]

Paint thinners are somatimes as dangerous as xylene is and less effective.
If you want additional information about eliminating xylene please read my 
articles at
www.histosearch.com/rene.html
 
René J.

From: Adrienne Anderson rennie1...@yahoo.com
To: Tom McNemar tmcne...@lmhealth.org 
Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu 
Sent: Wednesday, February 6, 2013 8:30 AM
Subject: Re: [Histonet] Xylene Substitutes

Thanks so much, everyone, for the feedback. 

I have a colleague who is convinced paint thinner is exactly the same as the 
SubX we use. Does anyone know if this is true?

Thanks again,
Adrienne


On Feb 6, 2013, at 5:36 AM, Tom McNemar wrote:

 Not familiar with SubX but we have used Americlear for many years with good 
 results.  You just can't beat Xylene for some things though and still keep a 
 little around.
 
 Tom McNemar, HT(ASCP)
 Histology Co-ordinator
 Licking Memorial Health Systems
 (740) 348-4163
 (740) 348-4166
 tmcne...@lmhealth.org
 http://www.lmhealth.org/
 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne 
 Anderson
 Sent: Tuesday, February 05, 2013 4:58 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Xylene Substitutes
 
 Hello all,
 
 My lab is looking into xylene substitutes, and I'd love some feedback on what 
 other labs are using. We currently use SubX, but are there other items out 
 there more economical?
 
 Thanks,
 Adrienne
 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 
 This e-mail, including attachments, is intended for the sole use of the 
 individual and/or entity to whom it is addressed, and contains information 
 from Licking Memorial Health Systems which is confidential or privileged. If 
 you are not the intended recipient, nor authorized to receive for the 
 intended recipient, be aware that any disclosure, copying, distribution or 
 use of the contents of this e-mail and attachments is prohibited. If you have 
 received this in error, please advise the sender by reply e-mail and delete 
 the message immediately. You may also contact the LMH Process Improvement 
 Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be 
 secure or error-free as information could be intercepted, corrupted, lost, 
 destroyed, arrive late or incomplete, or contain viruses. The sender 
 therefore does not accept liability for any errors or omissions in the 
 contents of this message, which arise as a result of e-mail transmission. 
 Thank
 you.


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[Histonet] Xylene Substitutes

[Beth Austin-Sell]

Adrienne,

If you'd like information on Formula 83 please contact me off list at
b...@cbgbiotech.com 

 

Thanks, 

Beth Sell

CBG

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RE: [Histonet] Xylene Substitutes

[Dessoye, Michael J]

We used SubX but have switched to Clear-Rite 3 from Thermo Fisher with
excellent results as well as cost savings.

Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General
Hospital | An Affiliate of Commonwealth Health |
mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre,
PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 
 

-Original Message-
From: Adrienne Anderson [mailto:rennie1...@yahoo.com] 
Sent: Tuesday, February 05, 2013 4:58 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Xylene Substitutes

Hello all,

My lab is looking into xylene substitutes, and I'd love some feedback on
what other labs are using. We currently use SubX, but are there other
items out there more economical?

Thanks,
Adrienne
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RE: [Histonet] Xylene Substitutes

[Blazek, Linda]

We have used Formula 83 from CBG for years with excellent results.  We use it 
for processing and staining.  We also recycle it making the cost very low.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dessoye, 
Michael J
Sent: Wednesday, February 06, 2013 11:47 AM
To: Adrienne Anderson; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Xylene Substitutes

We used SubX but have switched to Clear-Rite 3 from Thermo Fisher with 
excellent results as well as cost savings.

Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital 
| An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 
N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 
570-552-1526 
 

-Original Message-
From: Adrienne Anderson [mailto:rennie1...@yahoo.com]
Sent: Tuesday, February 05, 2013 4:58 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Xylene Substitutes

Hello all,

My lab is looking into xylene substitutes, and I'd love some feedback on what 
other labs are using. We currently use SubX, but are there other items out 
there more economical?

Thanks,
Adrienne
_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

This email and any files transmitted with it are confidential and intended 
solely for the use of the individual or entity to whom they are addressed.
If you have received this email in error please notify the originator of the 
message. This footer also confirms that this email message has been scanned for 
the presence of computer viruses.

Any views expressed in this message are those of the individual sender, except 
where the sender specifies and with authority, states them to be the views of 
Commonwealth Health.

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Email Security software in conjunction with virus detection software.



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Re: Re: [Histonet] Xylene Substitutes

[ewj]

We have a small lab at a university in Beijing where we do diagnostic 
histopath for swine diseases.
The lab is shared with graduate students who make histomorphologic 
measurements on tissues like gut and
muscle as part of their research.  We have a Sakura autostainer.  Last 
summer one student left all of the
caps off of all of the containers in the DRS autostainer over a hot 
summer weekend and

vaporized a very large amount of xylene and ethanol into the
room and out into the hallway which is on a floor shared with clerical 
staff one of whom was in early
stage pregnancy.  There was quite a furor ensuing, and no matter what 
the cause, the foreigner in the
building and his heavy use of the laboratory was to blame.  I have the 
support of the leadership/management
at the building and weathered the incident but it was not a comfortable 
situation.


Reducing the use of xylene is very attractive to us.

We have now been using Rene's hot water detergent method for some time.  
I have noticed that
when I do the process we get more reliable removal of the paraffin than 
if my staff does it.  Also one
of my workers has small sensitive hands.  My hands are toughened up from 
growing up on a
farm and working in the oil fields, and working with cattle and horses 
for quite a few years,

so the hot water doesnt bother me much,
but it is a problem for her and she is an excellent and valuable staff 
member

and really I should be concerned about scalding any one.

Rene' had suggested that I could get one of those restaurant coffee 
'vats' with a spigot on the bottom
to make and hold the hot water detergent solution, but those are not so 
easy to come by.  I have found
some old sterilizers which are essentially glorified pressure cookers 
with some automatic control that were
cast aside. These I have rigged up with pt100 thermocouples and PID 
thermocontrollers and use them to heat the water.
They have a drain port so I have also affixed them with heat tolerant 
solenoid valves for input and to drain

the slide container, and water level sensors, all of which are connected
to a computer driven data access module via relays so that process can 
be automated  and repeatable and
have tweakable cycles.  It's easier for me to procure such stuff in 
Beijing than it is to find t-shirts that fit me.


This also provides ready access to crisp hot water which is great for 
cleaning up paraffin
 from tissue block molds and tables and floors,  clearing plugged 
cleaning lines from the VIP5 (like when students fail

to drain the retort prior to retrieving their cassettes), and what not.
We dont have hot running tap water in the university lab.  (It's a 
luxury in homes as well but

much more common in recent years.)

I havent got around to converting the VIP5 to 2-propanol and mineral 
oil.  It's on a different
floor with good ventilation and I have enough trouble getting the 
students to read the sign
to drain the retort (in chinese), so I am not needing another level of 
complexity there

just yet.

I have been fiddling with the detergent solution and have found what 
does not work and also
found a reasonable result with an imported powdered dishwasher soap.   
We used a water
bath at first which is a real pain to use, is slow, and unpredictable.  
Predictable hot water

is important.

We find much fewer problems with lost sections particularly brain
with this method than with the xylene-alcohol series.  We do pig work 
primarily

so we run lots of brain sections looking for PRV and CSF and strep.

I am glad that Rene' continues to push his xylene-free concept.  It has 
helped me

immensely

Wayne Johnson
Enable Ag Tech Consulting
Beijing

On 3:59, Rene J Buesa wrote:

The best solution to eliminate xylene is to use isopropyl alcohol mixed with 
mineral oil.
Xylene can be eliminated from staining by dewaxing with 2% aq. sol. of 
dishwasher soap.
Before coverslipping oven dry the stained sections and cover directly.
To clean tissue processors use a 2% mixture of strong lab lab-ware detergent.
Do all of the above and your lab will be xylene free.
René J.

From: Tom McNemartmcne...@lmhealth.org
To: 'Adrienne Anderson'rennie1...@yahoo.com; 
histonet@lists.utsouthwestern.eduhistonet@lists.utsouthwestern.edu
Sent: Wednesday, February 6, 2013 5:36 AM
Subject: RE: [Histonet] Xylene Substitutes

Not familiar with SubX but we have used Americlear for many years with good 
results.  You just can't beat Xylene for some things though and still keep a 
little around.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
http://www.lmhealth.org/


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne 
Anderson
Sent: Tuesday, February 05, 2013 4:58 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Xylene Substitutes

Hello all,

My lab is looking into xylene

[Histonet] Xylene Substitutes

[Adrienne Anderson]

Hello all,

My lab is looking into xylene substitutes, and I'd love some feedback on what 
other labs are using. We currently use SubX, but are there other items out 
there more economical?

Thanks,
Adrienne
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[Histonet] Xylene/substitutes Validation/verification

[Stephanie Rodriguez]

Liz,

You are correct. If you bring in an FDA-approved test with NO
modifications, all that is required is verification; you simply have to
verify that the test works in your lab when you follow the recommended
procedure.  Validation, which is required for laboratory-developed tests
as well as laboratory-modified FDA-approved tests, is, indeed, a much
lengthier process.

Stephanie Rodriguez, HTL(ASCP), QIHC
Lead Molecular Technologist-FISH
IHC Technologist III
Phenopath Laboratories
Seattle, WA
(206) 374-9000 



On 9/27/11 9:49 AM, histonet-requ...@lists.utsouthwestern.edu
histonet-requ...@lists.utsouthwestern.edu wrote:

Message: 8
Date: Tue, 27 Sep 2011 10:32:07 -0600
From: Elizabeth Chlipala l...@premierlab.com
Subject: RE: [Histonet] Xylene sensitivity
To: 'Rene J Buesa' rjbu...@yahoo.com, Histonet Listserv (E-mail)
   histonet@lists.utsouthwestern.edu, ShelleyD'Attilio
   sdatt...@stormontvail.org, Loralee AMcMahon
   loralee_mcma...@urmc.rochester.edu
Message-ID:
   14E2C6176416974295479C64A11CB9AE1DECBA0A63@SBS2K8.premierlab.local
Content-Type: text/plain; charset=iso-8859-1

Rene

Rene

Loralee is correct in some aspects and you have addressed this already
with mentioning validation.  This basically has to do with the internal
validation that is required when you bring these kits into the
laboratory.  When you modify a 510K cleared kit or FDA approved kit it
becomes a LDT or Laboratory Developed Test there is significant
additional validation required because by changing the process you have
essentially increased the risk.  Additional validation is required as
defined by CLIA.  The manufacturer of the kit has gone through a process
that essentially has decreased the laboratory risk when then get a kit
FDA cleared or approved, if the lab chooses to modify this kit they
essentially have increased their risk significantly and are required to
do additional validation, more than would be required if you used the kit
as recommended.

I'm thinking that all that is required if you use a cleared or approved
kit is verification and not validation, but I may be incorrect.
Validation is a more intense process.  Patty from Phenopath or Beth
Shepard from Ventana would be able to comment on this process also.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504




This e-mail message, including any attachments, is for the sole use of the 
intended recipients and may contain privileged information. Any unauthorized 
review, use, disclosure or distribution is prohibited. If you are not the 
intended 
recipient, please contact the sender by e-mail and destroy all copies of the 
original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. 
at (206) 374-9000.


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[Histonet] RE: Histonet- Xylene Substitutes

[Beth Austin]

Vanessa, 
I work with a lot of great histology folks and what I know about it is that
eosin bleeding and blue-haze problems usually occur as a consequence of
either not fully removing all of the water during the final absolute alcohol
rinse step, and/or not fully removing all of the alcohol during the clearing
step.  If the water and alcohol are completely removed, bleeding and haze
will not occur. I hope this helps. 

Beth Austin-Sell
CBG Biotech



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
histonet-requ...@lists.utsouthwestern.edu
Sent: None
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 80, Issue 4

Send Histonet mailing list submissions to
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than Re: Contents of Histonet digest...


Today's Topics:

   1. Re: control tissue for Hall's bile stain (Robert Richmond)
   2. RE: BILLING QUESTION (Cynthia Pyse)
   3. RE: ER and PR validation (jmye...@aol.com)
   4. Thomas Crowell is out of the office. (thomas.crow...@novartis.com)
   5. Looking for MIN6 cell line (Debra Baluch)
   6. bat wing histology (Amos Brooks)
   7. RE: BILLING QUESTION (Feher, Stephen)
   8. xylene substitute (Vanessa Avalos)


--

Message: 1
Date: Fri, 2 Jul 2010 13:48:52 -0400
From: Robert Richmond rsrichm...@gmail.com
Subject: [Histonet] Re: control tissue for Hall's bile stain
To: histonet@lists.utsouthwestern.edu
Message-ID:
aanlktildu0qipr9rjajgqqonoaanf2yk1skc0houk...@mail.gmail.com
Content-Type: text/plain; charset=ISO-8859-1

Hall's bile stain - not really a stain but a reaction, like the Perls
prussian blue reaction for iron - uses trichloracetic acid and ferric
chloride to oxidize yellow-brown bilirubin to bright green biliverdin.
Or so I've been told - I've never seen one.

Something I've wondered about for a long time: could Hall's reaction
be used to demonstrate meconium in macrophages in the chorion of the
human placenta? These can be hard to see, particularly if you don't
have a very good microscope. - If this would work, it would provide a
ready source for controls, at least for the hospital pathologist.

Bob Richmond
Samurai Pathologist
Knoxville TN



--

Message: 2
Date: Fri, 2 Jul 2010 13:51:51 -0400
From: Cynthia Pyse cp...@x-celllab.com
Subject: RE: [Histonet] BILLING QUESTION
To: 'Sara Baldwin/mhhcc.org' sbald...@mhhcc.org,
histonet@lists.utsouthwestern.edu
Message-ID: 01cb1a0f$40ca1c10$c25e54...@com
Content-Type: text/plain;   charset=us-ascii

We have a contract with the hospitals, this comes from our billing manger.
We bill the hospitals, then they bill the patient. 

Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cp...@x-celllab.com



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sara
Baldwin/mhhcc.org
Sent: Friday, July 02, 2010 11:59 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] BILLING QUESTION

Histonetters:
If you do some work for another hospital (Histology) can you bill the
hospital or do you have to bill the patient directly?  Is there a statute or
Regulation out there about this?

Thanks
Pathology Supervisor
Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center
sbald...@mhhcc.org
Ph 812-482-0210, 482-0216,  Fax 812-482-0232, 
Pager 812-481-0897
Confidential information, Authorized use only.

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--

Message: 3
Date: Fri, 2 Jul 2010 13:54:26 EDT
From: jmye...@aol.com
Subject: RE: [Histonet] ER and PR validation
To: histonet@lists.utsouthwestern.edu
Cc: dorothy.l.w...@healthpartners.com
Message-ID: 8f84.3cbd32f0.395f8...@aol.com
Content-Type: text/plain; charset=US-ASCII

This paper can also be downloaded, indirectly, from the CAP's website:
 
_http://www.archivesofpathology.org/doi/pdf/10.1043/1543-2165-134.7.e48_ 
(http://www.archivesofpathology.org/doi/pdf/10.1043/1543-2165-134.7.e48) 
 
 
Happy (re-)validating,
Joe
 
--

Message: 12
Date: Fri, 2 Jul 2010 10:23:08 -0500
From: Webb, Dorothy L dorothy.l.w...@healthpartners.com
Subject: [Histonet] ER and PR validation
To: _'histonet@lists.utsouthwestern.edu'_ 
(mailto:'histonet@lists.utsouthwestern.edu') 

In a recent 

[Histonet] Xylene substitutes

[Dawn Oakes]

What are labs using for xylene substitute. I will be using it in a hand
staining setup for Mohs. Feed back on  Histo-Clear,  Clear solve, S-3
and formular 83.

Thanks in advance.

Dawn Oakes HT

 


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Re: [Histonet] Xylene substitutes

[Brandi Higgins]

We use Formula 83 and are very happy with it.  We use it exclusively in the
VIP processor and to deparaffinize before staining.  We also use as clearing
agent after staining, prior to coverslipping, but have found that we still
need the last container before coverslipping to be xylene for best results.
Otherwise a film develops, and air bubbles are more prominent.  I tried
switching to another xylene substitute because it was slightly less
expensive, but it was not satisfactory and we went straight back to Formula
83.

Hope this helps.
Brandi Higgins, B.S. HT(ASCP)
On Wed, May 12, 2010 at 10:02 AM, Dawn Oakes doa...@olympicmedical.orgwrote:

 What are labs using for xylene substitute. I will be using it in a hand
 staining setup for Mohs. Feed back on  Histo-Clear,  Clear solve, S-3
 and formular 83.

 Thanks in advance.

 Dawn Oakes HT




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 Confidentiality Notice:  This e-mail message, including any attachments, is
 for the sole use of the intended individual(s) named above and may contain
 confidential, privileged, and/or protected information.  Any unauthorized
 review, use, disclosure, copying, or distribution of its contents is
 prohibited.  If you are not the intended recipient, you have received this
 email in error.  If so, please notify the sender immediately by reply email
 and delete/destroy the original and all copies of this communication.  Also
 know that Internet e-mail is not secure.  In choosing to communicate with
 Olympic Medical Center by email you will assume these confidentiality risks.
   Internet messages may become corrupted, incomplete, or may incorrectly
 identify the sender.
 ___
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[Histonet] xylene substitutes

[Perry, Margaret]

We use formula 83 for everything and do not have a problem with a film.  We 
have found that we must use isopropal alcohol instead of reagent alcohol in the 
final dehydration steps.
Margaret Perry
SDSU

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Re: [Histonet] Xylene substitutes

[V. Neubert]

My hand staining setup contained some commercial naphta mix, which is
comparable to any other petroleum benzine solvant, for dewaxing.

For dehydrating/coverslipping, I used n-Butyl acetate a.k.a. butyl
ethanoate. Coverslipping can be done with any xylene soluble glue.


 What are labs using for xylene substitute. I will be using it in a hand
 staining setup for Mohs. Feed back on  Histo-Clear,  Clear solve, S-3
 and formular 83.

 Thanks in advance.

 Dawn Oakes HT

  


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 Confidentiality Notice:  This e-mail message, including any attachments, is 
 for the sole use of the intended individual(s) named above and may contain 
 confidential, privileged, and/or protected information.  Any unauthorized 
 review, use, disclosure, copying, or distribution of its contents is 
 prohibited.  If you are not the intended recipient, you have received this 
 email in error.  If so, please notify the sender immediately by reply email 
 and delete/destroy the original and all copies of this communication.  Also 
 know that Internet e-mail is not secure.  In choosing to communicate with 
 Olympic Medical Center by email you will assume these confidentiality risks.  
  Internet messages may become corrupted, incomplete, or may incorrectly 
 identify the sender.
 ___
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 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
   


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RE: [Histonet] xylene substitutes

[Martin, Gary]

We use Formula 83 ... we have had no problems in all phases.
Gary

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
njoydo...@aol.com
Sent: Wednesday, May 13, 2009 7:25 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] xylene substitues

just wanting to see what everyone's favorite xylene substitute is for
clearing slides before coverslipping?? 

Gene
Cleveland Clinic
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RE: [Histonet] xylene substitutes

[Blazek, Linda]

I second Gary's comments.  We use Formula 83 in all phases also.

Linda 

 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martin, Gary
Sent: Wednesday, May 13, 2009 10:50 AM
To: njoydo...@aol.com; Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] xylene substitutes

We use Formula 83 ... we have had no problems in all phases.
Gary

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
njoydo...@aol.com
Sent: Wednesday, May 13, 2009 7:25 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] xylene substitues

just wanting to see what everyone's favorite xylene substitute is for
clearing slides before coverslipping?? 

Gene
Cleveland Clinic
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