i dont know how people decide that this buffer ph ,salt concn,dtt,etc in
perticular concm is suitable for protein to be in folded state,i am doing
crystallisation of few proteins but i dont know how to select the parent buffer
for the protein,is it CD or crysatllisation trials that tells abt
Dear Atul,
for crystallisation, I would say the most suitable buffer is water.
If your protein concentrates well in pure water, why add anything else?
Of course, many proteins will not concentrate well in pure water, so
start adding buffer components until your protein is happy:
- buffer
Dear Engin
I would also like to comment. I our recent structure determination of
the sodium pump (3.5 A) (see morth JP et al 2007) we did not have
experimental phasing to more than 6 A for the Ta6Br12 clusters and 7 A
for the Pt sites. Both with extensive multicrystal averaging and phase
Hello everyone,
I would like to install a system virtual machine to run Ubuntu Linux as
a guest OS on a 32-bit Vista laptop. The idea is to allow occasional
use of crystallographic refinement programs while I'm away from lab.
The laptop has an Intel Core 2 duo processor (2.0 GHz) and 3 GB
On Sun, May 10, 2009 at 08:24:34PM -0700, Engin Ozkan wrote:
The question is what would happen if your crystals diffract to 4 A,
and anomalous signal dies at 6 A. The interesting bit of course is 1
Met per 200 residue, which should put to death the 1 in 50 or 1
in 100 Methionine myths: it
Hi,
I was not implying to cut-out the bad pieces and apologize if it appeared as
such. In my short time in crystallography the lowest resolution I have worked
with is 1.95 A. This was only an example to help-out, and appeared to be the
best and complete answer to the question. Jason, I goofed
I'm using VirtualBox 2.2.2 to run a Ubuntu 9.04 on
WinXP (32 bit) as a testbed for migrating my lab servers/workstations
from Fedora 8. It runs surprisingly well with 512 Mbyte of assigned
memory and 64 Mbyte of assigned graphics memory. I have even used it to
test Wine to run some Win-only
Jason Porta jpo...@unmc.edu 05/09/09 3:19 PM
Hi everybody,
Sorry if this message was already asked (I could not find it in the
archives). I am making a figure of a recently solved protein structure
including the electron density. I would like the electron density to
cover only the
Many people responded with questions following our first
announcement of a ready-to-use small fragment screening library
designed for protein crystallographic screening. Given suitable crystals,
the methodology is of interest to anyone wishing to probe ligand
binding interactions and motifs
If experience from intrinsic zinc is ok, I'll add my two cents.
trying). I would be happy to hear about Se-Met cases, and data
collection strategies (2wl vs. 3wl MAD vs. SAD, etc.) and phasing
methods used in these cases, or references of them. Again, no other
Bert already mentioned
I just did the same today.
Those that are missing from the 4-03 patch (at least the src version)
included:
scala.f
version.fh
refmac5.tcl
scalepack2mtz.com
dtrek2mtz.com
pname2.com
import_scaled.script
also there was a tcl++ fix
William G. Scott
Contact info:
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