Hello Patrick,
On 02/07/2020 21:03, Patrick Loll wrote:
[...]
Does anyone have an estimate for the flow rate one would typically
use for the cold nitrogen stream passing over a protein crystal in a
standard data collection?
[...]
A Cryostream 700 works at 5 litres per minute (gas), I
Well I very much doubt that many software developers are going to trawl
through all their code, comments, output statements & documentation to
change 'redundancy' or 'multiplicity' to 'MPR' or whatever terminology is
agreed on (assuming of course we do manage to come to an agreement, which I
Hi Navdeep
Yes good point, the principle of redundancy (though they wouldn't have used
that term!) has a very long history, but von Neumann did more than anyone
before him to formalise it:
Sorry, way off topic:
Does anyone have an estimate for the flow rate one would typically use for the
cold nitrogen stream passing over a protein crystal in a standard data
collection?
Background: Our nitrogen “generator” has gone belly-up and the vendor no longer
services it, so I’m testing
Job opening for a structural biologist to join the Structural Motility team at
the Curie Institute Paris, France.
We are looking for a post-doctoral fellow to join the Structural Motility team
at the Curie Institute (Paris Center) directed by Anne Houdusse.
The Structural Motility team at the
There is probably some justification for the absence of 'reflection' (as used
in crystallography) - in 'purist' Physics. The process
itself is not a 'reflection', despite that it can be macroscopically described
(in first approximation at least, and good enough for finding diffraction spot
Correction: It should read "with the detector almost but not quite
hitting the source."
On 02.07.20 17:13, Navdeep Sidhu wrote:
> Alexander Blake wrote a nice chapter on small-molecule crystallization
> in this book, if you run into problems in the crystallization stage:
>
> Alexander Blake.
Alexander Blake wrote a nice chapter on small-molecule crystallization
in this book, if you run into problems in the crystallization stage:
Alexander Blake. Crystal growth and evaluation (Chapter 3). In Clegg,
William (Ed.) Crystal Structure Analysis: Principles and Practice. 2nd
Edition.
Dear all,
I am writing regarding a problem we are facing with the refinement of a
structure. We would really appreciate it if anybody could suggest how to
set up geometrical restraints for a protein-DNA covalent bond in Refmac or
Phenix?
Thanks in advance,
Best wishes,
Cristina
--
Cristina
Dear all,
The MepAnti project aims to tackle the antimicrobial resistance problem by
targeting enzymes of the methylerythritol phosphate pathway. The MepAnti
consortium consists of nine academic partners and seven non-academic
partners coordinated by The Helmholtz Institute for Pharmaceutical
Dear Ian,
You seem to be slightly off there: The successful use of repeating
observations to reduce (especially systematic) observational error
predates von Neumann by at least 4 centuries.
One of the first instances of its use was in the 1500s, due to a migrant
scientist working in Denmark and
Dear all
I’ve been persuaded that MPR is a useful name (and see that there are
shortcomings with both “multiplicity” and “redundancy") and I agree with much
of what’s been said most recently in this thread.
BTW, just because the Physics definition of a measurement/quantity/whatever is
given
Thermo Fisher sells His-tagged GFP quite cheaply.
https://www.thermofisher.com/order/catalog/product/A42611#/A42611
Tristan
On 2020-07-02 10:31, Mark J van Raaij wrote:
Dear All,
Wondering if anyone knows of an economical commercial source of a soluble His-tagged protein. In principle any
Dear Luca,
Thanks for your email. CCP4i is no longer supported by the CCP4 Core Team.
The main interfaces in CCP4 7.1 are CCP4i2 and the CCP4cloud client.
However, ccp4i is still included with the suite, as you found, and can be
run from the command line if you source the CCP4 environment in a
Dear all,
While following the development of this thread, I am truly amazed how people
cling to names for the number of measurements per reflection whose meaning:
* Depends on the cultural/engineering/scientific context
* Can only be understood by experts
* Where the experts, as
Dear Mark,
this is a small spin-off company from my Dept. I am not personally
involved, but as they are biochemists they produce biophysics quality
proteins and they have the expertise to discuss your needs.
All the best,
Beatrice
--
Prof.
Hi Mark,
NEB
Dear All,
Wondering if anyone knows of an economical commercial source of a soluble
His-tagged protein. In principle any His-tagged protein would suffice, because
it's for testing coupling to a surface via the His-tag. Asking for an
international collaborator, who don't have access/expertise
It is hard for us to rise above these cultural differences, so perhaps the
adoption of a third, precisely-defined, and neutral term is indeed
warranted. MPR seems a good start, but it forces us to think about what we
mean by measurement. My feeling is that a diffraction spot can be
measured in
Good morning Jose,
The devil is always on the detail:-
You are of course correct that I had presumed, as Ethan pointed out, a sub 10
fsec pulse.
Neutrons creating magnetic waves, you are again correct, “spin echo“ does
occur, but without damage though as neutrons have such gentle energies
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