Hi Careina,
if you don't have grey hair (available in the lab), you can still mount
crystals at room temperature. With a MiTeGen RT kit, very little skill is
required to test crystal diffraction or even collect entire data sets at
room temperature.
https://www.mitegen.com/product/micrort-room-tem
Wouldn’t that be something for the PyMOL mailing list?
All best - Andreas
On Fri, 9 Feb 2018 at 20:38, David Schuller wrote:
> http://www.cell.com/current-biology/fulltext/S0960-9822(18)30014-9
> A Novel Form of Stereo Vision in the Praying Mantis
> Vivek Nityananda ,Ghaith Tarawneh, Sid
> Henri
Dear Nishant,
Rosetta is a good suggestion. You can also use an ensemble of several
related (superposed) structures as your search model. This will improve
your chances of success.
All best.
Andreas
On Tue, Aug 29, 2017 at 12:50 PM, Nishant Varshney wrote:
> Dear Crystallographers,
>
> I
Dear Rhys,
I second Roger on the use of jelly-body refinement. In addition, give
Buster a try. It sometimes does magic at lowish resolution.
All best.
Andreas
On Thu, Jul 13, 2017 at 1:17 AM, Rhys Grinter
wrote:
> Dear All,
>
> I'm currently in the process of refining a low(ish) resoluti
Hi Sebastian,
you're thinking about local sparse matrix screening. I've done this at a
90:10 ratio.
Majeed, S., Ofek, G., Belachew, A., Huang, C.C., Zhou, T., and Kwong, P.D.
Enhancing protein crystallization through precipitant synergy.
Structure. 2003; 11: 1061–1070
All best.
Andreas
On
Hi FBDD Evangelist,
Emerald Bio is now Rigaku Reagents. https://www.rigakureagents.com/
They don't seem to sell the pHAT screen on their website, but they might be
able to provide you with a PDF of the compositions.
All best.
Andreas
On Mon, Mar 6, 2017 at 10:56 AM, Hernani Silvestre
wrot
Dear Andre,
I agree with Jacob that P 42 21 2 might be the right space group, and that
and R free of 33% isn't so bad. If your electron density is poor, that
might just reflect the low resolution. Have you tried refinement with
Buster? It has a way with low-resolution data.
All best.
Andreas
Dear Wei,
if you process your data with XDS, the best is probably to do the scaling
in XDS (CORRECT) and be done with it. If you want to use Aimless for
merging, you can turn off scaling with the ONLYMERGE keyword or use SCALES
CONSTANT.
All best.
Andreas
On Thu, Nov 17, 2016 at 9:40 PM, W
Dear Deepa,
there was a time when spaces in the names of foldera and files was a big
nono in ccp4. Maybe it still is. Try changing New Folder to New_Folder or
something else without a space.
Best
Andreas
On Wed, Jun 3, 2015 at 6:09 AM, Deepa Raju wrote:
> Dear all,
> I have installed ccp
Hi Eric,
What does your map look like? Do you see features that don't come from the
search model? That's the key. That said, with a TFZ of above 10, I'd be
rather positive about my prospects.
Andreas
On Tue, May 19, 2015 at 2:36 AM, Eric Karg <
052044071b36-dmarc-requ...@jiscmail.ac.uk
Hey Tommi,
I am under the impression that Zbyszek Otwinowski has looked in depth
at all of the structures that have now been retracted and has prepared
a long manuscript detailing the evidence for fabrication and
falsification. As far as I know, this manuscript hasn't been
published yet (shame!),
Dear all,
I'm converting intensities from scala to amplitudes with ctruncate like so:
ctruncate -mtzin scala_protein_3_001_180.mtz -colin "/*/*/[IMEAN,SIGIMEAN]"
The data are native. An mtz file is generated, and it looks ok, but
ctruncate doesn't terminate properly. Instead, after Anisotropy
As Warren pointed out, dual-boot is so 20th century it's surprising people
still bother with it. For me, dual boot (never mind it was on a fantastic
Thinkpad) was the major reason to go for OSX. I was simply too sick of
it.. It might sound like heresy to true Macolytes but I feel I have now the
Depends on your robot, obviously.
On Mon, Apr 14, 2008 at 10:32 PM, William Scott <[EMAIL PROTECTED]>
wrote:
> Does this include the customary grieving period?
>
>
>
> On Apr 14, 2008, at 2:10 PM, JOE CRYSTAL wrote:
>
> Hi,
> >
> >
> > Does anyone have information about how long it takes to set
Hey Ethan, all,
On Feb 6, 2008 1:02 AM, Ethan Merritt <[EMAIL PROTECTED]> wrote:
> The problem is that both Fedora and Suse 10.3 currently ship with a
> broken xorg library. This is a known problem (Google for details),
> but I do not know if there is a fixed version available for download.
No
To avoid excessive excitement potentially caused by such a list, people
should also indicate the time spent with a model just good enough to
initially justify the eventually futile effort.
Andreas
On 9/21/07, Bryan W. Lepore <[EMAIL PROTECTED]> wrote:
>
> would anyone be willing to share storie
You'd need quite a large French press or meat grinder to crack the cells
and get the protein.
William Scott wrote:
On Thu, 20 Sep 2007 17:23:05 +0100
"R. J. Lewis" <[EMAIL PROTECTED]> wrote:
a large signalling complex called the 'stressosome' from B. subtilis.
Before going into an ethics class, I think this material needs to go into a
crystallography class. Every crystallographer (and maybe even every
structural biologist) should know why the structure is fishy, how fishiness
can be detected, how one can make sure one's own structure is legit, etc.
Anal
For the benefit of overworked and stressed-out graduate students and
post-docs, funding agencies need to be convinced that the remotes cannot
be bought without the console.
Andreas
Stephen Graham wrote:
Full VR systems with motion tracking and are now affordable to most labs
(~$2500 for all
This is an old version of the c++ libraries. You get it by installing
compat-libstdc++-33
Andreas
Paul Kraft wrote:
Hiys,
I've got an hp pavillion dv2000 with a 64x2 processor and I loaded the
redhat version of CCP4-6.0.2. I tried loading it manually but there was
no configure or make file
Hello Klaus,
I think you should give Gerard some "Hofnarrenfreiheit". (That's a fine
German word for you to figure out, Gerard.) He is certainly not an evil
racist at heart.
Andreas
Klaus Piontek wrote:
Greetings (or in "correct" Swiss German "Grüezi wohl", with Umlaut=vowel
mutation) t
Dear all,
two days ago I asked the ccp4 and 3DEM lists how one could prevent carbon
films from being destroyed by detergent. At least that's what I meant to
ask. Thanks for the many responses I got in no time (summarized below).
Thanks to all who responded and apologies to those I don't mention
Hey all,
this is wildly off-topic, but since we were even talking about NMR the other
day, I was thinking why not EM?
I'm trying to get negative stain images of a membrane protein. Problem is
that the detergent (similar to triton) that best stabilizes the protein eats
the carbon grid, even at c
Steve,
Steve Lane wrote:
Given the current situation at Apple, particularly their shift in focus
and revenue percentage from "computers" to other types of devices,
i.e. iPods, either/both of the above reasons for refusal to do the work
are plausible.
are you saying one shouldn't buy one's scie
Hey all,
let me give this discussion a little kick and see if it spins into outer
space.
How many reflections do people use for cross-validation? Five per cent is a
value that I read often in papers. Georg Zocher started with 5% but lowered
that to 1.5% in the course of refinement. We've had
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