Hi Careina, if you don't have grey hair (available in the lab), you can still mount crystals at room temperature. With a MiTeGen RT kit, very little skill is required to test crystal diffraction or even collect entire data sets at room temperature. https://www.mitegen.com/product/micrort-room-temperature-starter-kits/
All best. Andreas On Tue, Aug 14, 2018 at 12:48 PM, Harry Powell < 0000193323b1e616-dmarc-requ...@jiscmail.ac.uk> wrote: > Hi > > One other thing to try that someone with grey hair in your lab might know > about - mount a "crystal" in a capillary and see if it diffracts at room > temp. As long as you have a source and detector in your home lab, there's > no need to go to a synchrotron and use their in situ facilities. > > There are those of us who would contend that if your sample doesn't > diffract, then it isn't a crystal, no matter how nice it looks (though it > might have been one once...)! > > Harry > -- > Dr Harry Powell > Chairman of European Crystallographic Association SIG9 (Crystallographic > Computing) > > > > > On 14 Aug 2018, at 11:14, Elspeth Garman wrote: > > Yes, essential to test at room temperature without changing their buffer > medium before getting worried! > If they don’t diffract at RT, they are very very unlikely to diffract at > cryotemperatures, whatever you do to them before hand! > Best wishes > Elspeth > > *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of > *Hughes, > Jon > *Sent:* 14 August 2018 11:11 > *To:* CCP4BB@JISCMAIL.AC.UK > *Subject:* [ccp4bb] AW: [ccp4bb] crystals that dont diffract :( :( > > maybe it's the cryobuffer that's the problem (you didn't mention it). you > could try to fish the crystals with minimal liquid attached by mounting > them in oil rather than a cryobuffer. or you could test the native > diffraction "in situ" (at room temperature in the drop): quite a few > beamlines offer this possibility these days. > best > jon > > *Von:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK > <CCP4BB@JISCMAIL.AC.UK>] *Im Auftrag von *Careina Edgooms > *Gesendet:* Dienstag, 14. August 2018 11:59 > *An:* CCP4BB@JISCMAIL.AC.UK > *Betreff:* [ccp4bb] crystals that dont diffract :( :( > > I got the most beautiful crystals I have ever seen and they don't diffract > at all. Not poor diffraction, NO diffraction. Anyone know why this could be > and how I can go about fixing it? I had three beautiful crystals and not > one diffracted. I did leave them in the drop for about 3 weeks before > harvesting and in liquid nitrogen for about a month before diffracting. > Could that be a factor? If I regrew more beautiful crystals and diffracted > straight away could that help? > Careina > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
