Re: [ccp4bb] Mac mini advice

Cara you have re-ignited the perennial Mac v PC debate!!! You'll be asking about depositing raw diffraction data next ;) Cheers Ashley Sent from my iPhone On 23/01/2013, at 10:29 PM, Anastassis Perrakis a.perra...@nki.nl wrote: I am of the opinion that the truth lies somewhere in between ...

Re: [ccp4bb] IUCr committees, depositing images

in the Acta Cryst TARDIS paper a couple of years ago (http://scripts.iucr.org/cgi-bin/paper?S0907444908015540) particularly the usefulness of raw data availability for methods development, so I'm really happy to see continued active discussion in this area! Cheers Ashley Buckle Steve Androulakis

Re: [ccp4bb] not so good news (Steve Jobs RIP)

Bill So eloquently put, much of what you say resonates with me. Very sad day. Cheers Ashley Sent from my iPhone On 06/10/2011, at 3:13 PM, William G. Scott wgsc...@ucsc.edu wrote: On Oct 5, 2011, at 5:52 PM, Bosch, Juergen wrote: http://www.apple.com/stevejobs/ May innovation continue to

[ccp4bb] Examples of images from various detectors

Dear All I am looking for example files of most common detector formats eg from the mosflm doc (below). I have some of the most common ones we use routinely eg Mar, ADSC and R-Axis but if anyone has by chance compiled an archive of example files, or can point me in the right direction that

Re: [ccp4bb] Structure Based Sequence Alignment

On 26/05/2010, at 6:53 AM, Jürgen Bosch jubo...@jhsph.edu wrote: http://ww2.cs.mu.oz.au/~arun/Site/mustang.html We have built a web server for this at http://pxgrid.med.monash.edu.au:8080/mustangserver/ Cheers Ashley

Re: [ccp4bb] Structure Based Sequence Alignment

! Thanks Dhirendra On Tue, May 25, 2010 at 4:05 PM, Ashley Buckle ashley.buc...@med.monash.edu.au wrote: On 26/05/2010, at 6:53 AM, Jürgen Bosch jubo...@jhsph.edu wrote: http://ww2.cs.mu.oz.au/~arun/Site/mustang.html We have built a web server for this at http

Re: [ccp4bb] Finding best model for molecular replacement

This is generally a good idea, but removal of residues is a subjective process, and a little trial and error. If your sequence searching finds multiple search models you can superimpose them and systematically remove the poorer fitting regions based upon RMSD. We have built a server for this

Re: [ccp4bb] software to represent raw reflections in hkl zones

hklview will generate pseudo precession images Sent from my iPhone On 05/05/2010, at 11:03 PM, Tillmann Heinisch tillmann.heini...@unibas.ch wrote: Hi, I have problems solving the structure of a protein crystal which seems to be disordered. In order to investigate the disorder it would

[ccp4bb] TARDIS v2: a central, searchable index for federated raw crystallography data

We are pleased to announce the release of TARDIS v2 at http://tardis.edu.au TARDIS v2 provides a central, searchable index for federated raw crystallography data. If you are interested in the safe storage and simple annotation of your diffraction data (eg as soon as it comes off the

Re: [ccp4bb] Refolding of Denatured Protein

Have a look at REFOLD: http://refold.med.monash.edu.au/ cheers Ashley On 05/05/2009, at 9:27 PM, Sanjiv Kumar wrote: I am working on a membrane protein. There was problem with the normal purification of the protein so I have tired to purify it under denaturing conditions (Using 8M Urea).

[ccp4bb] TARDIS v2 beta released

We are pleased to announce the release of TARDIS v2 beta at http://pxgrid.med.monash.edu.au:8000/ TARDIS v2 provides a central, searchable index for federated raw crystallography data. Improvements over TARDIS v1 (http://tardis.edu.au): - Data is no longer stored in a Fedora Digital

Re: [ccp4bb] long term data backup

We are working on a new version of TARDIS that massively simplifies the software requirements (no database needed), using Web Stores. We are planning to release this at the beginning of April (but not the 1st!) See http://tardis.edu.au/wiki/index.php/TARDIS_Web_Stores In a nutshell:

Re: [ccp4bb] buster-tnt on OSX ?

Having solved a few structures using AutoSHARP, I feel compelled to comment on the CCP4BB that I really like the software, never had a major problem installing or using it, feel extremely grateful to the guys at Global Phasing for making this available to us all, and for (in my case at

Re: [ccp4bb] how to promote folding for an unfolded protein

Jenny have a look at our REFOLD database: http://refold.med.monash.edu.au you can find several refolding protocols in there for immunoglobulin folds (you can blast your sequence against the refold database) cheers ashley -- Ashley Buckle Ph.D NHMRC Senior Research Fellow The Department

Re: [ccp4bb] need test data set

with most of the ccp4 programs in the wiki. (during recent bioxhit ,oulu workshop) where can i get them, is there any scientist ready to provide. -- S.Jayashankar Research Student Institute for Biophysical Chemistry Hannover Medical School Germany Ashley Buckle Ph.D NHMRC Senior Research Fellow

Re: [ccp4bb] Two off-topic questions

proteins are more stable than human ones? Any suggestions would be greatly appreciated. Thank you for your suggestions in advance! Best wishes Wei Yong Ashley Buckle Ph.D NHMRC Senior Research Fellow The Department of Biochemistry and Molecular Biology School of Biomedical Sciences, Faculty

Re: [ccp4bb] multiple sequence alignment from multiple pairwise structural alignments

Nuffield Medical Fellow Division of Structural Biology Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Phone: +44 1865 287 549 Ashley Buckle Ph.D NHMRC Senior Research Fellow The Department of Biochemistry and Molecular Biology School of Biomedical Sciences

Re: [ccp4bb] The importance of USING our validation tools

is _expensive_! Unmerged intensities are probably more manageable Phil On 16 Aug 2007, at 15:05, Ashley Buckle wrote: Dear Randy These are very valid points, and I'm so glad you've taken the important step of initiating this. For now I'd like to respond to one of them, as it concerns something I

Re: [ccp4bb] The importance of USING our validation tools

as raw data? Rawest are the images - everything beyond that is modellling - but archiving images is _expensive_! Unmerged intensities are probably more manageable Phil On 16 Aug 2007, at 15:05, Ashley Buckle wrote: Dear Randy These are very valid points, and I'm so glad you've taken

Re: [ccp4bb] Inclusion body

to know where to get the plasmids. Any help or comments would be appreciated. Weijun -- Petra Verdino, PhD Research Associate The Scripps Research Institute, BCC206 10550 North Torrey Pines Road CA 92037 La Jolla, USA tel:1-858-784-2294 fax:1-858-784-2980 Ashley Buckle Ph.D NHMRC Senior Research