[ccp4bb] AW: [ccp4bb] Off topic - French Press Accessories

hi naza, i wouldn't touch machines like that from the USA – their prices are too high and their tolerances are miserable. we bought a made-to-measure French press (including various stainless-steel cells) from a REAL hydraulics manufacturer near here https://watzhydraulik.de/en/ about 20 years

[ccp4bb] ABP1 has no known function

Dear all, Many people know much more about the plant hormone auxin than I do, but I believe I am right in saying that, despite Romana Gáborová's current PDBe highlight on auxin binding protein 1 (ABP1) at https://www.ebi.ac.uk/pdbe/about/news/growth, there is currently no valid evidence that

[ccp4bb] PhD position: 3D structure/function of phytochrome photoreceptors

I have a position for a PhD student to work in my lab PhD position Phytochrome structure/function A PhD position at the Institute for Plant Physiology of the Justus Liebig University is available immediately. The position (E13 65%) is funded for three years as part of the project

[ccp4bb] AW: [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

in my experience using silane with PAGE plates, rain-x is much better (at least in that case). jon Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Goldman, Adrian Gesendet: Donnerstag, 31. Januar 2019 17:43 An: CCP4BB@JISCMAIL.AC.UK Betreff: Re: [ccp4bb] Is there any

[ccp4bb] AW: [ccp4bb] AW: [EXTERNAL] [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

yes, rain-x is excellent (also for PAGE gel plates). a little bottle costs almost nothing and will last you a lifetime. best jon Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Nagarajan V Gesendet: Donnerstag, 31. Januar 2019 16:14 An: CCP4BB@JISCMAIL.AC.UK Betreff: Re:

[ccp4bb] AW: [ccp4bb] 3D stereo and pymol

...just open pymol, go to display - stereo mode - cross-eye then click stereo. it might need a little practice and it makes one look even sillier than one does usually, but it works, costs nothing, needs no updates and i'm told that it's even good for your eye muscles! best, jon Von: CCP4

[ccp4bb] honeybee repair?

hi everyone, we use a honeybee pipetting robot for 96-well sitting-drop screening – or rather we would like to do so. several of the pins are defective, replacements seem not to be available, our workshop has not been able to fabricate them successfully. the only possibility would seem to be

[ccp4bb] AW: [ccp4bb] [Offtopic] Protein stain in Coomassie Blue dyes

hi, as a comment to zhijie's question, the biggest problem with marion bradford's "protein" assay is that it measures the SDS concentration too! therefore, where it would be most useful – that is in quantifying the total protein in an SDS-treated gel sample – it cannot be used! the amido-black

[ccp4bb] AW: [ccp4bb] Electron density maps for Cryo-EM structures.

yes, but irrespective of how much one knows or thinks one knows, one should avoid being gratuitously offensive. cheers jon Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Ian Tickle Gesendet: Montag, 10. September 2018 12:58 An: CCP4BB@JISCMAIL.AC.UK Betreff: Re: [ccp4bb]

[ccp4bb] AW: Re: [ccp4bb] crystals that dont diffract :( :(

Yes indeed! Jon -- Jon Hughes (+49/0)1757929098 Sent without the use of Apple products. Daniel M. Himmel, Ph. D. schrieb Dear JL, Years ago, this was a common problem when I was crystallizing myosin constructs for my doctoral work. Some of the most beautiful crystals I got showed

[ccp4bb] AW: [ccp4bb] crystals that dont diffract :( :(

maybe it's the cryobuffer that's the problem (you didn't mention it). you could try to fish the crystals with minimal liquid attached by mounting them in oil rather than a cryobuffer. or you could test the native diffraction "in situ" (at room temperature in the drop): quite a few beamlines

[ccp4bb] AW: [ccp4bb] AW: [ccp4bb] [ccp4bb] Oxford University Press

er every 4 years to ensure grant money continues to flow in. F. From: CCP4 bulletin board mailto:CCP4BB@JISCMAIL.AC.UK>> on behalf of Hughes, Jon mailto:jon.hug...@bot3.bio.uni-giessen.de>> Sent: Saturday, June 30, 2018 12:12:50 PM To: CCP4BB@JISCMA

[ccp4bb] AW: [ccp4bb] [ccp4bb] Oxford University Press

sorry about the typing error. 2 x 200 = 400, i know. j -Ursprüngliche Nachricht- Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Hughes, Jon Gesendet: Samstag, 30. Juni 2018 12:13 An: CCP4BB@JISCMAIL.AC.UK Betreff: [ccp4bb] AW: [ccp4bb] [ccp4bb] Oxford University

[ccp4bb] AW: [ccp4bb] [ccp4bb] Oxford University Press

8, at 8:11 AM, Hughes, Jon > wrote: > > whose paper? our universities pay subscriptions for these journals and we > even pay on top of that for the pages of our publications (even when they're > not actually printed!), whilst we review papers for free! sounds like a > well

[ccp4bb] AW: [ccp4bb] Oxford University Press

whose paper? our universities pay subscriptions for these journals and we even pay on top of that for the pages of our publications (even when they're not actually printed!), whilst we review papers for free! sounds like a well-validated way to use taxpayers' money to keep the expensive company

[ccp4bb] AW: [ccp4bb] Inquiry - active and non-active state in alternatives

dear petr, several phytochrome structures seem to represent mixed-state crystals. best, jon -Ursprüngliche Nachricht- Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Petr Kolenko Gesendet: Dienstag, 11. Juli 2017 16:00 An: CCP4BB@JISCMAIL.AC.UK Betreff: [ccp4bb]

[ccp4bb] PhD position - Phytochrome structure/function

Dear collegues, I would be grateful if you were to draw the attention of appropriate persons to the following flyer (if the fancy html formatting doesn't work, here's the flyer in PDF:

[ccp4bb] AW: [ccp4bb] Sliconizing of cover-slips

anyone tried rainex (if it's still on sale)? for us it works 1000x better than anything else: rugged, extremely water repellent, and cheap (one bottle lasts a lifetime). in the '90s at least you could get it in auto stores in the USA. cheers jon Von: CCP4 bulletin board

[ccp4bb] AW: [ccp4bb] UVEX UV Fluorescence

ah! ok, that's a different matter j Von: Keller, Jacob [mailto:kell...@janelia.hhmi.org] Gesendet: Mittwoch, 5. April 2017 14:29 An: Hughes, Jon <jon.hug...@bot3.bio.uni-giessen.de>; CCP4BB@JISCMAIL.AC.UK Betreff: RE: [ccp4bb] UVEX UV Fluorescence But I think he was asking about i

[ccp4bb] AW: [ccp4bb] UVEX UV Fluorescence

for ethidium bromide you need a 590nm (50-100nm FWHH) bandpass interference filter. ccd chips are sensitive to infrared and transilluminators produce a lot of it! best j Von: Keller, Jacob [mailto:kell...@janelia.hhmi.org] Gesendet: Mittwoch, 5. April 2017 14:16 An: Hughes, Jon <jon.hug...@b

[ccp4bb] AW: [ccp4bb] UVEX UV Fluorescence

you can save a fortune by just fitting a 590nm interference filter to the front of a ccd camera attached to a PC + a UV/B transilluminator. i set this up in my lab for about €1000 total 15 years ago and we've used it everyday since. cheers jon Von: CCP4 bulletin board

[ccp4bb] AW: [ccp4bb] [ccp4bb] protein precipitation reg

9) > > >Isn’t the whole point to use a small amount of buffer so you can easily >push the pH around in crystallization screens? (At which point the >sample is usually in 100+ mM buffer.) > > >On Mar 29, 2017, at 2:03 PM, Hughes, Jon ><jon.hug...@bot3.bio.uni-giessen.de&g

[ccp4bb] AW: [ccp4bb] [ccp4bb] protein precipitation reg

yes, "oil of vitriol" is sulphuric acid, "blue vitriol" is copper II sulphate as i recall. j -Ursprüngliche Nachricht- Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von CRAIG A BINGMAN Gesendet: Donnerstag, 30. März 2017 05:52 An: CCP4BB@JISCMAIL.AC.UK Betreff: Re:

[ccp4bb] AW: [ccp4bb] protein precipitation reg

...it's just a wonderful tradition! there's an interesting description of the history of tris in maniatis cheers jon Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von David Briggs Gesendet: Mittwoch, 29. März 2017 17:53 An: CCP4BB@JISCMAIL.AC.UK Betreff: Re: [ccp4bb]

[ccp4bb] AW: [ccp4bb] How to determine the concentration of biotinylatedpeptide?

very good point! you need lots of protein and it has to be pure (meaning also minimal buffer, salts and stuff) but it worked pretty well in our hands (when we were trying to measure the extinction coefficient of phytochrome). incidentally, I think that the notion of quantitative amino acid