Re: [ccp4bb] cryo for high salt crystal

rking in a cold room to harvest your crystals will help reduce the evaporation rate. Good luck! Best,Anna On Tue, Jul 10, 2012 at 9:28 AM, m zhang wrote: regaentDear All, I am sure this question was discussed before. But I am wondering if anyone got the same experience as I do. I got a

Re: [ccp4bb] cryo for high salt crystal

You could also try high salt solutions with similar technique. Good luck! Jim From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of m zhang [mzhang...@hotmail.com] Sent: Tuesday, July 10, 2012 11:28 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] cryo for high salt

[ccp4bb] cryo for high salt crystal

regaentDear All, I am sure this question was discussed before. But I am wondering if anyone got the same experience as I do. I got a crystal out of condition with 1M KCl, 1.4M Ammonium sulfate at pH7. I tried to use glycerol, ethylene glycol, 25% sucrose, paraton-N oil, or ammonium sulfate itse

Re: [ccp4bb] AW: [ccp4bb] crystallization of complex and ...

e e-mail and do not disclose its contents to any person. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited.Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von m zhang Gesendet: Freitag, 16. September 2011 04:20 An: CCP4BB@JISCMAIL.AC.UK

[ccp4bb] crystallization of complex and ...

Dear all, I have two questions: First, I was trying to crystallize a complex of two proteins. Both proteins has been crystallized before. The two proteins bind to each other based on Biacore study, but they didn't form a single peak on gel filtration. When I mixed them at 1:1 ratio, the cry

Re: [ccp4bb] stoichiometry of complex and incubator

this helps. Regards, Krishna Krishna Chinthalapudi Graduate Student Hannover Medical School Germany On Thu, Aug 4, 2011 at 4:32 AM, m zhang wrote: Dear all, I have two unrelated questions. Any suggestion on any of them will be greatly appreciated. First, we have two proteins

[ccp4bb] stoichiometry of complex and incubator

Dear all, I have two unrelated questions. Any suggestion on any of them will be greatly appreciated. First, we have two proteins that bind each other without a doubt. But since we have very limited amount of proteins and it takes a long time to reproduce them, we are very hesitating to try

[ccp4bb] setting up additive screen

Dear all, I am trying to optimize my crystal with additives. Since the yield of my protein purification is very limited, I am wondering what is the most efficient way to set up drops with additive to save my protein and not wasting additives? I am setting up 1 to 1 drops with 0.2 ul additiv

Re: [ccp4bb] low expression and aggregation of protein

example, temperature, cuture, etc. Best, Lin On Fri, Jan 21, 2011 at 8:12 PM, m zhang wrote: Dear All, Recently I am trying to express a glycoprotein in Drosophila S2 cells. However, the expression yield is every low and most of the protein expressed aggregate. I was suggested making new

[ccp4bb] low expression and aggregation of protein

Dear All, Recently I am trying to express a glycoprotein in Drosophila S2 cells. However, the expression yield is every low and most of the protein expressed aggregate. I was suggested making new constructs. But I am wondering if anyone here can give me some suggestions to improve it beyond tha

[ccp4bb] model-mask

Hi All, I was trying to make a model-mask file for ncs-averaging in DM. I noticed that in CNS, the mask file has to be O-compressed. Does that mean if I read in O the mask file and write it out, the output file is already in O-compressed format? Related to the first question, the second on

[ccp4bb] questions about CNS

Hi everyone, I have some basic questions about CNS. First, I am wondering if there is anywhere I can set my initial B-factor during my early refinement. When I generate my initial model in generate.inp, I can set the B-factore. However, after I did the rigid.inp and anneal.inp, the B-factor just