Re: [ccp4bb] [QUAR] Re: [ccp4bb] embarrassingly simple MAD phasing question (another)

2010-10-16 Thread Kay Diederichs
Am 20:59, schrieb James Holton: ... The loss of the 1/r^2 term arises because diffraction from a crystal is compressed into very sharp peaks. That is, as the crystal gets larger, the interference fringes (spots) get smaller, but the total number of scattered photons must remain constant. The

Re: [ccp4bb] [QUAR] Re: [ccp4bb] embarrassingly simple MAD phasing question (another)

2010-10-15 Thread James Holton
Actually, people forget the 1/r term because it is gone by the end of Chapter 6 of Woolfson. Yes, it is true that, for the single reference electron the scattered intensity falls off with the inverse square law of distance (r) and, hence, the amplitude falls off with 1/r. However, the units

Re: [ccp4bb] [QUAR] Re: [ccp4bb] embarrassingly simple MAD phasing question (another)

2010-10-14 Thread Ethan Merritt
On Thursday, October 14, 2010 12:12:18 pm Lijun Liu wrote: I think I need make it clear. Not their changes (f' and f) but their contribution to reflection intensities changes. f' and f are not changes. They are the real and imaginary components of anomalous scattering. They are wavelength

Re: [ccp4bb] [QUAR] Re: [ccp4bb] embarrassingly simple MAD phasing question (another)

2010-10-14 Thread Bart Hazes
On 10-10-14 01:34 PM, Ethan Merritt wrote: ... The contribution from normal scattering, f0, is strong at low resolution but becomes weaker as the scattering angle increases. The contribution from anomalous scattering, f' + f", is constant at all scattering angles. ... My

Re: [ccp4bb] [QUAR] Re: [ccp4bb] embarrassingly simple MAD phasing question (another)

2010-10-14 Thread Ethan Merritt
On Thursday, October 14, 2010 01:18:04 pm Bart Hazes wrote: On 10-10-14 01:34 PM, Ethan Merritt wrote: ... The contribution from normal scattering, f0, is strong at low resolution but becomes weaker as the scattering angle increases. The contribution from anomalous scattering, f'

Re: [ccp4bb] [QUAR] Re: [ccp4bb] embarrassingly simple MAD phasing question (another)

2010-10-14 Thread Jacob Keller
I have always found this angle independence difficult. Why, if the anomalous scattering is truly angle-independent, don't we just put the detector at 90 or 180deg and solve the HA substructure by Patterson or direct methods using the pure anomalous scattering intensities? Or why don't we see

Re: [ccp4bb] [QUAR] Re: [ccp4bb] embarrassingly simple MAD phasing question (another)

2010-10-14 Thread Tim Gruene
On Thu, Oct 14, 2010 at 04:28:26PM -0500, Jacob Keller wrote: I have always found this angle independence difficult. Why, if the anomalous scattering is truly angle-independent, don't we just put the detector at 90 or 180deg and solve the HA substructure by Patterson or direct methods using

Re: [ccp4bb] [QUAR] Re: [ccp4bb] embarrassingly simple MAD phasing question (another)

2010-10-14 Thread Ethan Merritt
On Thursday, October 14, 2010 02:28:26 pm Jacob Keller wrote: I have always found this angle independence difficult. Why, if the anomalous scattering is truly angle-independent, don't we just put the detector at 90 or 180deg and solve the HA substructure by Patterson or direct methods using

Re: [ccp4bb] [QUAR] Re: [ccp4bb] embarrassingly simple MAD phasing question (another)

2010-10-14 Thread William G. Scott
On Oct 14, 2010, at 2:28 PM, Jacob Keller wrote: I have always found this angle independence difficult. Why, if the anomalous scattering is truly angle-independent, don't we just put the detector at 90 or 180deg and solve the HA substructure by Patterson or direct methods using the pure