Re: [ccp4bb] Weird diffraction pattern

Dear all Thanks for all the input both on- and off- the list. We shall definitely look into these suggestions further and report again here in due course. Kind regards Sam On Tue, 9 Oct 2018 at 19:12, Sam Tang wrote: > Dear all > > Hello. We recently shot a crystal (a protein with small mole

[ccp4bb] AW: [EXTERNAL] [ccp4bb] Weird diffraction pattern

To me, it looks like some intergrown salt crystal. HS Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Sam Tang Gesendet: Dienstag, 9. Oktober 2018 13:13 An: CCP4BB@JISCMAIL.AC.UK Betreff: [EXTERNAL] [ccp4bb] Weird diffraction pattern Dear all Hello. We recently shot a

Re: [ccp4bb] Weird diffraction pattern

raised previously on CCP4bb From: Sam Tang Sent: 09 October 2018 15:02 To: Nave, Colin (DLSLtd,RAL,LSCI) Cc: ccp4bb Subject: Re: [ccp4bb] Weird diffraction pattern Hello Colin Although the unit cell dimensions from mosflm should be largely unreliable in this case, the software actually

Re: [ccp4bb] Weird diffraction pattern

*Sent:* 09 October 2018 12:13 > *To:* ccp4bb > *Subject:* [ccp4bb] Weird diffraction pattern > > > > Dear all > > > > Hello. We recently shot a crystal (a protein with small molecule as > ligand) at a synchrotron source and see a weird pattern. ( > https://drive.google.

Re: [ccp4bb] Weird diffraction pattern

Sam Would this unit cell index some of the spots? a = 7.00 ± 0.04 A, b = 9.96 ± 0.05 A, c = 6.29 ± 0.04 A. Colin From: CCP4 bulletin board On Behalf Of Sam Tang Sent: 09 October 2018 12:13 To: ccp4bb Subject: [ccp4bb] Weird diffraction pattern Dear all Hello. We recently shot a crystal (a

[ccp4bb] Weird diffraction pattern

Dear all Hello. We recently shot a crystal (a protein with small molecule as ligand) at a synchrotron source and see a weird pattern. ( https://drive.google.com/file/d/11bEtTJzKaAB5ZybezgN1cqBrckSRg2OV/view?usp=sharing ) Crystal was grown in Citric acid and ammonium sulfate, cryoprotected with gl

Re: [ccp4bb] weird diffraction pattern

Hello Tang, 1) For MR, you might want to try a range of homologs, or even a stack of overlapping homologs. A normal modes server like elNemo might also help if it can predict the "bend" your molecule undergoes upon binding. A long shot perhaps, but stranger things have happened. You also mig

Re: [ccp4bb] weird diffraction pattern

B@JISCMAIL.AC.UK Subject: Re: [ccp4bb] weird diffraction pattern Hi, Thanks to all who gave me suggestions concerning the weird diffraction pattern and I really appreciate it that Kay Diederichs help me processing my data set and answer my questions. Although the data set can be processed usi

Re: [ccp4bb] weird diffraction pattern

Hi, Thanks to all who gave me suggestions concerning the weird diffraction pattern and I really appreciate it that Kay Diederichs help me processing my data set and answer my questions. Although the data set can be processed using HKL3000, XDS without problems, the Rwork/Rfree values are still

Re: [ccp4bb] weird diffraction pattern

Dear Gerd, I wasn't really giving much attention to the poke between the ribs ;-) - for me the more serious matter was to see the merits of pixel detectors over CCDs made light of, as if they didn't really make much difference. If some people get carried away in the way you describe, we

Re: [ccp4bb] weird diffraction pattern

Dear Gerard, my "sound like a sales person" was meant as poking a little fun - nothing serious, of course. I and our users like our not-so-new-anymore Pilatus3 6M. It's a great detector in many ways. But, there is a lot of hype that this detector solves all-problem, for instance fine slic

Re: [ccp4bb] weird diffraction pattern

Thanks for spelling it out! Would that advice still hold if the mosaicity of the crystal is 0.7 degrees? (I know, I should go read the paper., but . . .) eab On 07/13/2017 03:00 PM, Gerard Bricogne wrote: Dear Gerd, I can assure you that I have no shares in Dectris nor any commecial conne

Re: [ccp4bb] weird diffraction pattern

Dear Gerd, I can assure you that I have no shares in Dectris nor any commecial connections with them. What I do have is a lot of still vivid memories of CCD images, with their wooly point-spread function that was affected by fine-grained spatial variability as well as by irredicible inaccura

Re: [ccp4bb] weird diffraction pattern

Dear Gerard, you sound like a sales person for Dectris. Fine slicing is perfectly fine with CCD detectors - it takes a bit longer because of the step scan instead of continuous scan. The read noise issue is often overstated compared to the sample induced scatter background. If for fine slicing

Re: [ccp4bb] weird diffraction pattern

Hi Chenjun, Few suggestions from my side. Process the data with XDS and look into acentric intensity distribution (it indicates any twinning possibility). Run XTRIAGE and SFCHECK to understand any twinning or pseudo translation possibilities. Twinning can confuse the program and suggest you smalle

Re: [ccp4bb] weird diffraction pattern

> -Original Message- > From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of ??? > Sent: Thursday, July 13, 2017 3:56 AM > To: CCP4BB@JISCMAIL.AC.UK > Subject: [ccp4bb] weird diffraction pattern > > hello everyone, > I would like to seek your opinion o

Re: [ccp4bb] weird diffraction pattern

Dear Tang, I noticed that your diffraction images seem to have been recorded on a 3x3 CCD detector. With this type of detector, fine slicing is often discouraged (because of the readout noise), and yet with the two long cell axes you have, any form of thick (or only semi-fine) slicing would r

[ccp4bb] AW: [ccp4bb] weird diffraction pattern

] weird diffraction pattern Hi David, Thanks for your comments. Although the spots become streaky in certain directions, I have processed the data in HKL3000 and imosflm, which suggested the C2221 space group (66.59, 246.95 and 210.17). The Rmerge(0.14), completeness(94.8%), redundancy(4.6) are OK

Re: [ccp4bb] weird diffraction pattern

Hi Jacob, I have tried seeding approaches but it didn't help. All best, Chenjun Tang

Re: [ccp4bb] weird diffraction pattern

Hi David, Thanks for your comments. Although the spots become streaky in certain directions, I have processed the data in HKL3000 and imosflm, which suggested the C2221 space group (66.59, 246.95 and 210.17). The Rmerge(0.14), completeness(94.8%), redundancy(4.6) are OK. When I tried to run Bal

Re: [ccp4bb] weird diffraction pattern

You've got multiple lattices--try seeding approaches mentioned in a recent/current thread. JPK -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of ??? Sent: Thursday, July 13, 2017 3:56 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] weird diffra

Re: [ccp4bb] weird diffraction pattern

.UK Subject: [ccp4bb] weird diffraction pattern hello everyone, I would like to seek your opinion on my crystal hits. I am working on a helicase of which the native structure is solved and the all solution statistics are fine. I am trying to crystallize and solve the structure of the protein/ssDNA

[ccp4bb] weird diffraction pattern

hello everyone, I would like to seek your opinion on my crystal hits. I am working on a helicase of which the native structure is solved and the all solution statistics are fine. I am trying to crystallize and solve the structure of the protein/ssDNA complex. I recently got some hits from c