RE: [Histonet] Does anyone have any ideas on the use of softener ondecal blocks. Does this help 'soften' them? If so how?

Floating the trimmed/faced block on the waterbath for 2 minutes helps. Then put them on wet ice to cool. Regards, Laurie. Mr. Laurie REILLY Histopathology School of Veterinary and Biomedical Sciences James Cook University Townsville Qld. 4811 Australia. Phone 07 4781 4468 -Original Mes

[Histonet] Re: Alcian Yellow

American Mastertech has a Helico*STAT stain kit that uses Alcian yellow solution and toluidine blue as staining components. If this is similar to what you are currently using, I am sure they would share their procedure with you if you called. I saw this stain at the Texas state meeting, and th

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Re: [Histonet] Isopropyl vs Ethanol

Amos: One disadvantage for manual processing with IPA is a little odor of "cat's urine". This is can fully eliminated when using VIP processor. We changed ethanol on IPA more than years ago and happy with it. We also uses mineral oil instead xylene and also happy. However none of our techs want not

RE: [Histonet] Antigen Retrieval

Try using a laboratory pressure cooker like the Decloaking Chamber from Biocare Medical. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PR

[Histonet] Antigen Retrieval

Hi- I am new to using IHC and I need some advice on Antigen Retrieval. I usually do it in an autoclave for 15min at 121C for FFPE human brain tissue (in Citrate or Tris Buffer), but by the end my tissue looks pretty bad and parts have even fallen off. The tissue is fixed on SuperFrost Plus Slid

Re: [Histonet] Winterize formalin?

Thank you Terry .. and thanks to everyone who replied to me about this ... Sate On Thu, Oct 2, 2008 at 10:37 AM, Marshall Terry Dr, Consultant Histopathologist <[EMAIL PROTECTED]> wrote: > 1.5% sounds homeopathic to me. > > There will be more depression of freezing point due to the salts in > bu

Re: [Histonet] Isopropyl vs Ethanol

Amos: Isopropyl alcohol (IPA) penetrates less quickly than ethanol, but dehydrates more gently and hardens the tissues less. When the tissue ihas been dehydrated with IPA, you can mixe it in a proportion of 5:1 and 2:1 with mineral oil at 50ºC and by doing so you can ELIMINATE --- On Fri, 10/3

Re: [Histonet] Isopropyl vs Ethanol

Amos: Isopropyl alcohol (IPA) penetrates less quickly than ethanol, but dehydrates more gently and hardens the tissues less. When the tissue has been dehydrated with IPA, you can mix it in a proportion of 5:1 (followed by another at 2:1) with mineral oil at 50ºC and by doing so you can ELIMINATE

[Histonet] damage to freeze-sections after cutting

Dear histonet users, I am just starting to do cryosections and I ran into a problem. I have mouse tissue that we freeze in OCT (we tried different tissue preprocessing: PFA immersion fix, whole animal PFA perfusion, sucrose gradient). I am sectioning skeletal muscle at 10 micron. When I look a

[Histonet] Isopropyl vs Ethanol

Hi, Is there anyone who uses exclusively isopropyl alcohol to process with rather than ethanol? I have had great results with isopropyl alcohol for some tissues, but I wanted to know if there are any pitfalls to using it exclusively for processing. I've noticed that isopropyl is much cheaper tha

Re: [Histonet] Does anyone have any ideas on the use of softener on decal blocks. Does this help 'soften' them? If so how?

Hi Sarah I don't use a softener as such, but have had good results putting trimmed blocks on a water soaked tissue on the bench for 30 min or so just before cooling and sectioning. I find it is more effective than an ice bath at softening tissues. REgards Piero Nelva Anatomical Pathology Mo