Hi Rosa,
Excuse me if I am wrong, but I think Bill is referring to using a microwave for
special stains, and you are referring to a Microwave Tissue Processor.
I have used an oven set at a higher temperature (85 -110 degrees C) for
staining the silver portion of the GMS, and it generally only
Thanks, Joe and Adam. I'm always paranoid about over tightening and
warping some piece of the equipment but maybe I'm being a little too
gentle. I'll make sure everything is nice and snug and see how it
goes.
Happy slicing...
Nate
Galbraith, Joe wrote:
Nathan:
As i
Nathan:
As indicated by others - first check that everything is tight as that is
most often the issue - don't forget to check the adhesion of the
specimen to the chuck as well. Also check the angle of approach to the
blade as it could be too steep or too shallow.
If everything is tight and prope
I was having this problem too, but I think I finally figured it out. There
is a screw on our cryostat that attaches the chuck to the rest of the
machine. This screws fits into a small hole in the chuck. Sometimes the
screw isn't well set in the hole or is well set but for some reason comes
loose an
Thanks, Robyn... I'll make sure the holder is clamped down. (sometimes
its the little things...)
Best
Nate
R J VAZQUEZ wrote:
Nathan,
It sounds like the blade holder is not secure enough or even in the
tightness on each side.
Hope this helps.
Robyn
>
Bill,
I can highly recommend the EBS processor microwave. At a base price of 8,900
(last I checked) it is one of the lowest cost processors on the market. I
don't know if having the flexibility to process in this new microwave may help
justify the price? I purchased mine from Stat Lab, and th
Everyone may want to check this out. Heard today that this is no longer the
case. Maybe Ventana will make an announcement!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi
Allison-Tacha
Sent: Thursd
Greetings Histonetters,
We have finally come to the point where we need to fish or cut bait in
relation to purchasing a laboratory approved microwave and venting it.
While I think it would be "cool" to have one, I'm wondering if the cost
is justified to do a handful of special stains that could
Nathan,
It sounds like the blade holder is not secure enough or even in the tightness
on each side.
Hope this helps.
Robyn
> Date: Thu, 27 Aug 2009 16:44:11 -0400
> From: natec...@gmail.com
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] uneven alternating sections on cryostat
Most people are unaware of this, but that's probably because Ventana
has had a contract with Cell Marque for years as their primary antibody
and other consumables source.
--- On Thu, 8/27/09, Maria Katleba wrote:
From: Maria Katleba
Subject: RE: [Histonet] Best IHC stainer
To: "godsgal...@aol.c
When cutting PFA fixed, cryoprotected tissue on our cryostat, I
frequently find that every other section gets cut improperly. I'll get
one nicely cut section and then on the next pass I only get half of a
section. This cycle simply repeats over and over and I lose many slices.
It has been a whi
We purchase our Alcian Yellow kit for Helicobacter from Newcomer Supply, Kit
#9130. Phone number is 800-383-7799. They are a wonderful company to deal
with and their Alcian Yellow kit works beautifully. Our pathologists love it!!
Lynne Bell, HT (ASCP)
Lead Histologist
Central Vermont Medical
I was wondering if anyone on the histonet uses an alcian yellow stain method
for H. Pylori? Do you have a problem finding the alcian yellow, or do you
use a substitute? I would be interested to hear your pros and cons on this
stain. Do you stain manually or use a kit? If a kit, where do you pur
William, thanks for the reminder, I had totally forgotten about using a
counterstain to counteract AF in aldehyde fixed samples. We have some
glutaraldehyde fixed cryosections of kidney I think I'll try this with. I can
assure you, the autofluorescence in these samples is magnificent! I worry ab
Dear Anne,
I assume the glutaraldehyde tissue has been processed and embedded in
paraffin. Following removal of paraffin and any pretreatment to unmask the
antigen, you can block autofluorescence or change the color by treating
the sections with 0.05% sodium borohydride prepared in PBS for 30 m
I am currently writing a procedure for the use of Toluidine Blue staining for
both FNA's and frozen sections at the request of one of our pathologists. Can
anyone guide me in the use of this stain in lieu of Diff Quick or H&E for rapid
processing (such as references I could use, etc) and does a
I suggest you use a counterstain for your IF to reduce the autofluoresence.
Evans Blue - the product can be used as a counterstain in immunohistochemistry
when using FITC. After staining for immunofluorescence, dip sections in a 0.1%
(w/v) in water solution of Evans Blue for 5-10 minutes. Rinse
I was a DAKO fan for years... But after using Ventana, I completely changed my
mind!
Sure you pay a little more, but honestly the amount is negligible! In fact,
after pricing out Dako versus Ventana, in the end the was really no difference.
Plus the quality of the Ventana is exceptional. No on
Anne,
Tissue that has been fixed in glutaraldehyde has very, VERY bright
autofluorescence. Unless there is some way to minimize this (none that I'm
aware of), your immunofluorescence will be impossible to read over the
background signal.
Teri Johnson, HT(ASCP)QIHC
Managing Director Histology F
We do the same and all of our readings have been well under what they should be.
Dawn D. Schneider, HT(ASCP)
Howard Young Medical Center
Woodruff, WI 54568
(715)356-8174
dawn.schnei...@ministryhealth.org
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histone
I gave out misinformation which I want to rectify.
The metal sleeves and holding tray is sold by McCormick Scientific which is a
part of Leica, but they have their own ordering website:
http://www.mccormickscientific.com/cassettemangement.asp?PCID=853
The above link should open to the item its
If anyone knows how to contact Michael, please email me. Thanks!
Phyllis Thaxton HT(ASCP)QIHC
DCH Regional Medical Center
Tuscaloosa, AL
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/his
Anne,
we do DIF on formalin fixed renal biopsies routinly. To get good results we
perform Protease digestion before DIF. I don't have the protocol here, but
we use Proteinase K from Dako at RT for 3-4 min, and the antibodies are
between 1:20 and 1:40 für 30 min.
I don't know, if that would help wit
We seperate biopsies and grossed tissue before grossing. They get into
differently coloured cassettes, and different VIPs. The cassettes of the
grossed tissue are put directly into the VIP baskets - short time dry, then
put into a container with formalin. While grossing a report is written. So
the
Having worked with Dako autostainer for years and currently using Vantana
Discovery XT and Leica Bond stainer's, my preference would be the Bond. You
have the capability of the Ventana stainer, and being able to unlock the
software that will allow you to use it as any other stainer on the market
Agreed. Intellipath from Biocare. Load it and walk away - and it has a
voice notification to tell you the run is finished. You can load it in
the PM, and take slides off in the AM. Love it. Has a chilling station
for AP chromogen, too.
Jacke O'
godsgal...@aol.com
Sent by: histonet-
We have GE Ultra (formerly Triple G) LIS. It is interfaced directly into
the printers. j
From: gre...@arlt-digital.de [mailto:gre...@arlt-digital.de]
Sent: Thursday, August 27, 2009 09:05
To: pat.b...@ucdenver.edu; Weems, Joyce;
histonet@lists.utsouthwestern.edu
Ventana reagents eem to be a little pricey, but if you want a system that you
can load and walk away from, it is the way to go. DAKO is an easy enough to
use instrument and is an open system, but they are going to a price per slide
kind of thing, if I remmeber correctly.
I prefer the IP fro
We load the cassettes into the tissue processing basket as they are grossed in.
They stay in a container of formalin until they are loaded on the tissue
processor. What concern about formalin exposure do you have with this method?
The gross hood is on while they are doing this. We also monito
We use Copath for Histology LIS and Epic as our Hospital and clinic based
system.
In Epic, you can retrieve who reviewed your results by looking at the result in
Chart Review. Look below the results, it lists who and when. There is also an
electronic trail that Epic can produce if needed.
Do
I prefer the DAKO. Check, besides the instrument itself, the reagents COST and
how "open" one system is compared with the other.
René J.
--- On Wed, 8/26/09, Gareth Blaeuer Davis wrote:
From: Gareth Blaeuer Davis
Subject: [Histonet] Best IHC stainer
To: histonet@lists.utsouthwestern.edu
Date:
Hi Joyce, Hi Pat,
>From my experience the printers are working very well. There could be issues
>if any of the adjustments are of or the slides or cassettes that are used not
>fit to the system. What kinds of problems do you having? What makes you
>unhappy with the printer?
@Joyce: I would like
Andrea,Go for: Vector Blue (Vector Labs) or Permanent Blue (Diagnostic
Biosystems) for AP activityVector Red or Permanent Red, also for AP activity.
These two AP immunostaining methods can be combined in a sequential double
staining method with a HIER step (10 min, 98C) in between for removing t
I echo Joyce's comments!
Greg
Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PEC1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385
"I find that the harder I work, the
more luck I seem to have."
- Tho
Dear John and other knowledgeable Histonetters and Gurus of the trade
Renal bx tissue core has been placed in Gluteraldehyde/Trumps EM
fixative by mistake (we all make mistakes) - we now need to do DIF
Does anyone out there have a method for this?
--
Anne van Binsbergen (Hope)
Abu Dhabi
UAE
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