I work with bone samples trimmed to approx 35mmx35mmx4mm. Our general procedure
is:
Fixation:
untrimmed samples stored in 10% NBF @4°C until trimming. Slices placed in fresh
10% NBF for at least 12h.
Decalcification
Benchtop - 10% formic acid for 10 days (change soln every 3 days), rinse for
I used to sterilze my TBS by autoclaving it.
For aliquots I used TBS with 0,1% sodium acide.
I never had any problems with bacteria; my 10x TBS concentrate was fine for
weeks.
- Original Message -
From: amosbro...@gmail.com
To: histonet@lists.utsouthwestern.edu
Date: 24.02.2010 17:59:45
Dear Laurie,
We manufacture the Clini-RF Rapid Freezer =80 degs. C with options for a
range of freezing methods.
Best Regards
Alan Bright
Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England
Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email:
Hello Histonet,
it has been a while (~10 months) since I posted a problem about uneven
immuno-staining with specimen showing unstained circles after manual staining
with HRP-polymere/DAB method; complete mail see below, response mails see
Histonet archive (via website).
Links to pictures I
Hi Laurie,
We distribute Bright's Clini-RF. We hear good reports about using 3MT NovecT
Engineered Fluid HFE-7100 which is inert and has an excellent environmental,
health and safety profile.
Regards,
Michael Williams
mjwilli...@scilogex.com
SCILOGEX, LLC
TF: (877) SCILOGEX
T: (860) 828-5614
I like this method too, and I would say it's one of the best. The only issue is
high throughput. When freezing 100+ samples the floating tray becomes much more
user friendly and believe it or not, the results are just as good.
--- On Wed, 2/24/10, Adam . anonwu...@gmail.com wrote:
From: Adam
Who supplies the 3MT Novec Engineered Fluid HFE-7100?
Thanks
Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On
It can be purchased directly from 3M. You may also find some local
suppliers.
Michael Williams
mjwilli...@scilogex.com
SCILOGEX, LLC
TF: (877) SCILOGEX
T: (860) 828-5614
F: (860) 828-5389
www.SCILOGEX.com
-Original Message-
From: Houston, Ronald
To me it seems that the sections after being picked from the water bath were
not completely drained and the dewaxing process was incomplete in a way that
the round areas kept certain amount of paraffin wax that prevented the
reagents reactions.
The fact that the areas are round are an
Hi,
There is a great hospital based Histotechnologists position now available in
the great midwestern city of Indianapolis, Indiana. Follow the link provided:
http://jobsearch.usajobs.gov/search.aspx?q=KB-10-SRC-320328where=brd=3876vw=bFedEmp=NFedPub=Yx=55y=14
Sincerely,
Nick Frain, AP
In response to the interested in other freezing techniques part of
Laurie's query: We snap freeze by throwing a cyrovial of tissue (or a
tissue piece wrapped in foil) into some liquid nitrogen for a couple
minutes (vials will float, foil-wrapped pieces will sink). For controlled
freezing of
Does anyone know how frequently you have to certify your NIST Thermometers (the
thermometer you use to calibrate all of the thermometers in the laboratory),
including a reference.
The certifiicate for my NIST Thermometer does not document the next time it
needs to be calibrated.
Thank you,
Ann
Folks,
I am looking for a control for some Flag-tag IHC I am doing on FFPE
tissue. I was wondering if someone could share some slides off of a
block of flag transfected cells.
THANKS!!
Julie
Julie Randolph-Habecker, Ph.D.
Staff Scientist - Director
Experimental Histopathology Shared Resource
Hello Histonet,
I am looking for a positive control for the Fluoro Jade Stain consisting
of rodent brain tissue demonstrating neuronal necrosis in the
hippocampus caused by administration of trimethyltin. Does anyone have
access to a stock of controls or can anyone tell me who to contact. I
I am in NY State and they just told me yesterday that we need to do it
annually.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From:
In the last two or three years I and others have posted a number of
times on Histonet about the Histobath (which is no longer in
manufacture), Bright's Clini-RF, and 3M's NovecT Engineered Fluid
HFE-7100. You can access these posts in the Archives, through Google
if you wish.
Bob Richmond
Samurai
Hello everyone!
Does anyone know where I can get more information about
haematoxylin-haematein-oxyhaematein chemistry?
I have a problem with my Harris haematoxylin modification (sodium iodate
as oxidant). When I use a smaller amount of sodium iodate (about 0,1g
per 1g of haematoxylin) I have a
Hello,
Can anyone using voice recognition with Cerner Millennium tell me what system
they are using and how they like it?
Thanks,
Jan Mahoney
Omaha, NE
Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent
Health is faithful to the
0.1G should give you an approximately half-oxidized hamatoxylin solution along
the lines of Baker's haematal-8 or haematal-16, or Gill's haemalum. It should
keep well. Precipitation must be due to something else you're doing.
For the chemistry a good starting point is Conn's Biological Stains
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