[Histonet] specimen disposal

Hi, can you tell me the correct/safe way to dispose of the formalin fixed specimens? The fixed specimens should be considered hazardous waste, but the formalin is a chemical hazard. I think having biohazard waste company that burns these specimens with formalin . According to MSDS we shoul

Re: [Histonet] ? about a Leica RM2155 microtome

The RM 2155 does need a foot pedal to run. We've had ours where the connection wasn't secure, it would stop the microtome when it wasn't enabled. My techs use it as a manual mictotome though. The foot pedal just sits behind the micotome. The next generation RM 2255 and RM 2265 don't need the foo

Re: [Histonet] mouse kidney frozen sectioning

Hi Andrew, You must freeze fast to avoid the needle like crystals forming from the water during freezing. Best - freeze in OCT in liquid Nitrogen. It gets worse if you keep changing the temp of the block. For example keep at -80 then bring to -20 cut than bring the temp down to remove the block

RE: [Histonet] Trichrome Help

Hi Drew, I don't know precisely what causes this, but I have seen it happen when using a freshly made batch of the dye solution. Also, a very fine whitish precipitate often collects in the bottom of the bottle in which the dye solution is stored, requiring filtration every day before using the so

[Histonet] Trichrome Help

I'm trying to troubleshoot a problem we had today with a Trichrome stain and I was wondering if anyone out there could help. We do Gomori Blue Collagen Trichrome Stain... 10 minutes in Weigert's Hematoxylin and then 15 min in the Gomori Blue Collagen stain followed by a quick change through acetic

Re: [Histonet] ? about a Leica RM2155 microtome

This is not true. You have to press the two blue buttons at the same time on the control panel to engage the motor. One button says run stop and the other says run enable. Remember that if you are in continuous mode you have to press the run stop button to stop the motor. Jack On May 3, 20

[Histonet] c1fe5960057c084ca389ce97779062904ebd0...@tcdmsg01.ad.texaschildrenshospital.org

What procedures do you need to know? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] mouse kidney frozen sectioning

Having trouble with freezing artifact in the form of tiny fissures or cracks in mouse kidney on frozen section. Tissue is paraformaldehyde fixed and infiltrated w 70% aqueous sucrose OCT solution. Anyone else seen this and know how to deal with it? Thx __

[Histonet] ? about a Leica RM2155 microtome

Is anyone out there using this machine? I was offered one without the foot switch. I was told this microtome will only work with the foot switch and cannot be operated manually. Can someone confirm this for me? Thanks Jim ___ James E. Staruk HT(ASCP) www.masshistology.com

RE: [Histonet] Switching Back to Formalin

I suggest Creative Waste Solutions for your Formalin recycling (actually refilering). We are a large volume lab and have been using multiple sytems (3 gallon to 20 gallon) for 5+ yrs without issue. By refiltering, you can reduce your purchase of new solution by at least 70%. There is no end po

RE: [Histonet] Switching Back to Formalin

Don't dispose of it... Recycle it. Creative Waste Solutions has a nice "Green" recycler for smaller labs, but we're using the BR Instrument for our lab. I never imagined that we would recycle our formalin, but when we tried out the BR, I was very impressed. We've saved so much by not having to c

[Histonet] Switching Back to Formalin

After years of working to get away from the use of Formalin in our lab, it seems as though we are going to be bringing it back. I'm seeking advice concerning what everyone is using to neutralize their formalin prior to disposal. Any recommendations welcome! Thanks in advance! Laura

[Histonet] Region II Symposium

Histonetter's: For anyone planning to attend the Region II SympoiumI wanted to give you a quick reminder that Monday May 10th, 2010 is the final day to get the discounted room rate at the Clarion Hotel for the Region II.. If you need information on the meeting you can find it through the NJ Soc

RE: [Histonet] Barcoding and Tracking Information

There are several systems that are out there. But I am glad to see that you are wanting to interface with the LIS. That is a key step. But I would look at all of them and come up with a pretty good RFP process for selection. Make sure also they are live with at least 3 to 4 sites with the inter

Re: [Histonet] keep deparaffinized and rehydrated slides in dH2O for a few days

No, I would not do that. Why don't you dehydrate again an put some drops of paraffin on it? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] keep deparaffinized and rehydrated slides in dH2O for a few days

Hi histonetters,   Is it safe to keep deparaffinized and rehydrated slides in dH2O at 4 degree for 4 to 5 days before doing IHC?   Thanks,   Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listin

[Histonet] RE: Feulgen/BrdU Dual labeling

Hi Group, I am re-posting my question from last week, I didn't not have many responses. If anyone has experience with this method, please don't hesitate to e-mail or call me. Is anyone out there doing Feulgen/BrdU Dual labeling? I have the procedure working (Blue Feulgen kit from Scytek) firs

[Histonet] CD133

Happy Monday! (grumble grumble) I need to know if there are any reference labs out there that do CD133 for stem cells. Our doc wants this done, but we can't find anyone who does it. Thanks Claire ___ Histonet mailing list Histonet@lists.utsouthwestern.e

[Histonet] Dimethyl sulfoxide

We purchase ours from Sigma-Aldrich. 1-800-558-9160 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the indivi

[Histonet] Barcoding and Tracking Information

Does anyone currently use an automated barcoding and tracking system that interfaces with CoPath-Sunquest?? If so can you please tell me which company you are using? Thanks so much Blake Taylor Section Supervisor Dept. of Pathology Lexington Medical Center 803-936-8214 bcdu...@lexhealth.org

[Histonet] Job Listing: Full time Position in West Columbia, SC

Histologist Full-Time 3AM - 11:30 AM Basic Functions: Prepares slides from surgical, outpatient and bone marrow specimens for examination by a pathologist. Perform special stains both by hand and thru automation. Embeds tissues into molds after processing, selecting proper size base mold an

RE: [Histonet] RE: Histonet Digest, Vol 78, Issue 2

I had one of my techs come to me one day saying that she thought I needed to look at her microtome because she had not been able to cut anything on her microtome today. She thought something had gone wrong with the tissue during processing. The other techs though were sectioning fine. This tech h

[Histonet] RE: Histonet Digest, Vol 78, Issue 2

That is a funny story, I would like to see the slide that was cut from a "blade holder section" with no blade in the clamp. I have heard it can be done but never see it myself. Though in old blade holders with loose clamps I have seen users get two blades in there at once. That didn't section

RE: [Histonet] CD68

I use both KP-1 and PGM-1. KP-1 is ordered about ten times as much as the PGM-1. Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Py