I'm still looking for a p/t tech to run a new GI path lab in Santa Rosa. Send
resumes to tja...@yahoo.com. Great facility, flexible hours, friendly docs,
warm, caring staff, and competitive pay.
Timothy Garcia-Jay
Pillar Consulting
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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
histonet-requ...@lists.utsouthwestern.edu
Sent: Thursday, 20 May 2010 3:35 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 78,
My wrongI meant epidermis. Blame old age
On Thu, May 20, 2010 at 12:08 AM, Andrew Burgeson nap...@siscom.net wrote:
One thing i forgot to mention wasthat when you embed, try
to orientate the
tissue so that the long axis (if there is one) lies in the
same direction as
the cutting
Please contact me at this email address. Thank you.
Sally Breeden, HT(ASCP)
Veterinary Diagnostic Services
New Mexico Department of Agriculture
700 Camino de Salud NE
Albuquerque, NM 87108
595-841-2576
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At 9:00 PM -0400 5/19/10, Robert Richmond wrote:
I probably shouldn't be admitting it, but I don't think I had enough
college science courses to be allowed to gross today. Maybe if they
don't find out that two of my biology courses were in paleontology. I
can gross a trilobite like you wouldn't
Hello,
I am currently making cryosections of transgenic tomato fruit expressing
mRFP
http://www.invitrogen.com/site/us/en/home/support/Product-Technical-Resources/Product-Spectra.mRFP.html
but am having some problems visualising fluorescence with a confocal. I
realise fluorescent proteins
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We require gloves for sterile sectioning as we are usually taking sections
for DNA and RNA (and a mask).Otherwise gloves etc are optional. Nothing for
embedding.
Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
ECOGPCO-RL
710 N Fairbanks Court
Olson 8-421
Chicago,IL
I would like to thank everyone for their contribution on how to remedy our
large fibrous bone tissue. Just for your knowledge, this case is a Congenital
Pseudoathrosis(CPT) and we have process them both for paraffin and MMA. The
paraffin was the choice for our study since we will be doing a lot
Help!
Does anyone know a substitute for sodium borate in the GMS stain? Ours got
unknowingly thrown out by our out-date police and he didn't tell anyone, and
now we need it ASAP. And we don't have half of the stuff for the Warthin-Starry
either. (Derm lab. We do this stuff oh so often, but now
Hello,
I want to stain sections of optic tectum to show the different
cellular layers. I am planning on using cresyl violet and luxol fast
blue. Anyone have any better ideas? I'm going to try HE as well
simply because everyone knows it.
Thanks
--
Tyrone Genade
http://tgenade.freeshell.org
20 Mule Team Borax in the laundry soap aisle. ;)
From: Ingles Claire cing...@uwhealth.org
To: histonet@lists.utsouthwestern.edu
Sent: Thu, May 20, 2010 9:47:50 AM
Subject: [Histonet] Sodium borate?
Help!
Does anyone know a substitute for sodium borate in the
HISTOTECHNOLOGIST
ProPath, a high volume, pathology practice, located in Dallas, Texas, has an
immediate opening for a Histotechnologist.
Responsibilities include embedding tissue specimens, microtomy of
paraffin-embedded tissue, operation of automated stainer and coverslipper,
equipment
TBS has an electric 3 well forceps warmer catalog #FW-120
Message: 3
Date: Tue, 18 May 2010 10:26:17 -0700
From: Pat Laurie foreig...@gmail.com
Subject: Re: [Histonet] alternative for bunsen burner?
To: Brandi Higgins brandihigg...@gmail.com
Cc: histonet@lists.utsouthwestern.edu
Message-ID:
We normally coverslip our immunofluorescence slides with a permanent
aqueous mounting medium. By mistake, today they were coverslipped with
a mounting medium that we use for coverslipping our FISH slides. This
mounting medium is described as Mounting medium for fluorescence, with
propidium
I think that the propidium iodide will stain the nuclei or DNA, similar
to DAPI, so this mounting media will counterstain the nuclei and its
visible in the FITC wavelength (488), while if you conterstain with DAPI
it is excited with a different wavelength, therefore it might interfere
with the
Hi Laurie,
Propidium iodide is a DNA intercalator that fluoresces red (ex 488nm).
Regards,
Merced
--On Thursday, May 20, 2010 11:13 AM -0700 Laurie Colbert
laurie.colb...@huntingtonhospital.com wrote:
We normally coverslip our immunofluorescence slides with a permanent
aqueous mounting
Histonetters:
Here's a message I was asked to post..
Dear Colleagues,
I have the following question concerning tissue processing. We do a lot of
IHC work on NF fixed tissue. To standardize and minimize the effect of NF
fixation, we fixate the tissue always for 24h. This is of course a
Actually if I could make a minor correction to your statement: propidium
iodide is excited by green light at 488nm but emits in the red portion of
the spectrum (620nm)...
--On Thursday, May 20, 2010 12:48 PM -0600 Liz Chlipala
l...@premierlab.com wrote:
I think that the propidium iodide
Concerning Immunofluorescence, where are you getting your (PBA) Protein
Blocking Agent from? I use to order it from Thermo Shandon but they no longer
carry it.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On
We just use Dako's serum free protein block for IF.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado
Please help, we have had an increase in demands for repeats on our AFB stain.
Our pathologists have been noticing what they believe are false positives on
our slides. We perform our AFBs on the Ventana Nexus and some are done by hand.
Because of this problem, we have been doing side by side
Hello Laurie
Pi gives red color in IF, it stains DNA and RNA
PI excites at 535nmand emits at 615nm, producing a *red* fluorescence.
Therefore if you are using any chromophore at these wave lengths which emits
red color you will have problems.
By the way we also have several mounting mediums for
I was talking to Peggy Wenk over the weekend at the MSH meeting and they
had a paper that was published regarding fixation and ER/PR staining
sensitivity etc. The biggest problem that they reported is
underfixation is much worse than over fixation. I think a minimum of 10
hours of fixation
From what I have seen and heard you can have fixation times up to= 72
hours and still be ok?
Sarah Goebel, B.A., HT (ASCP)
= div
Histo= technician
XBiotech USA Inc.
8201 East Rivers= ide Dr. Bldg 4 Suite 100
Austin, Texas 78744
(512)386-5107
How often are you decontaminating your NeXes machines (running
decontaminating reagents through the tubing)?
Jan Shivers
UMN VetDiagLab
- Original Message -
From: debra.or...@uchospitals.edu
To: histonet@lists.utsouthwestern.edu
Sent: Thursday, May 20, 2010 2:41 PM
Subject:
We fix all specimens in NBF for 24h, wash them in tap water and store them
in alcohol 70% at 4°C until further processing. That works perfectly well
for us, and we never noticed any loss of staining.
But I also knew that some colleagues prefer to run a weekend program on
their Tissue Tek without
I just realized the question came from Belgium. I have no idea how they do
things there.
Mark
On Thu, May 20, 2010 at 2:14 PM, Mark Tarango marktara...@gmail.com wrote:
Yes, you can go up to 72 hours for ER/PR (new CAP/ASCO guidlines), but if
you do HER2 the maximum is still 48 hours. I'm
Yes, you can go up to 72 hours for ER/PR (new CAP/ASCO guidlines), but if
you do HER2 the maximum is still 48 hours. I'm assuming you want HER2 as
well, so your best option would probably be to hold the tissues in 70%
alcochol on the processer until Sunday night.
Mark Tarango
On Thu, May 20,
We decontame once a month.
Mike
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jan
Shivers
Sent: Thursday, May 20, 2010 3:23 PM
To: debra.or...@uchospitals.edu; histonet@lists.utsouthwestern.edu
Subject:
Yeah. Good luck with that one. The minimum clearly states 6 hrs.
Mike
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
sgoe...@xbiotech.com
Sent: Thursday, May 20, 2010 3:08 PM
To: LINDA MARGRAF
Cc:
The problem with all this is that the ER/PR fixation times and the her2
fixation times do not match 72 hrs vs 48 hrs. Many times the same
specimen that receives ER/PR also gets her2. This is where things are
missed up. Why would they ever change one and not the othe...@#$%^*
Mike
-Original
Are you using tubing on your dispensers for your DI water? They can be a source
of contaminant. You can also have cross contaminantion from your control slide
during dehydration and hydration as well as during staining.Do not use the same
set up for contrpl and pt slide. Lay slides flat for
I heard on a teleconference yesterday that they're going to be changing both
to 72 hours max time fixation, but until its the published guidlines 48
hours still stands for her2neu.
Mark
On Thu, May 20, 2010 at 2:28 PM, Mike Pence mpe...@grhs.net wrote:
The problem with all this is that the
Hi Histonet Subscribers,
Are you interested in hearing about new job opportunities? I am a one of the
founders of a Healthcare Recruiting firm that specializes in placing Lab
Professionals. We work exclusively on permanent positions and have established
relationships with clients at
The CAP has the following to say about this issue (in the FAQ section
on HE= R2 testing):
The CAP Immu= nohistochemistry
Committee does not advocate
transferring formalin-fi= xed tissues into
alcohol. There are
no data to support the validity= of testing specimens
We discussed this problem of weekend fixation of breast tissue (about 54 hrs in
10% NBF) with our Reference Lab and they reassured us that ER/PR IHC wouldn't
be a problem. However, when ordering Her2, select FISH method instead of IHC.
(Otherwise, if it is fixed for less than 48 hrs we would
Dear Colleagues:
Please keep in mind that the ASCO/CAP guidelines are recommendations, not
mandates. You can experiment, validate, and establish your own
formalin-fixation protocol for breast specimens. At Hartford Hospital I have
established a minimum of 4.5 hours for needle core specimens.
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