Hi Laurie,
I have had success keeping the nails on using gelatin coated slides. I make up
the gelatin in beaker and dissolve it using the microwave. Then coat the slide
and pick the section from the water bath using the the just coated slide. I
then dry the slide overnight in the 37degree
Hi
In our fairly large histopathology lab we're hoping to consolidate our
autostainers and coverslippers to only two (Sakura) machines.
In order to efficiently do this, we would need to bypass the
autostainer's oven so that multiple racks of slides can be processed
continuously without any
Use the sakura DRS hotpot
Loading from the plate (apart for the other drawbacks) will set the wax on the
first slides put in the racks and you may end uo with inadequate dewaxing
Just my 5cents worth
Annieinarabia
Empower your Business with BlackBerry® and Mobile Solutions from Etisalat
The correct answer is b: if there is water underneath the section that is
going to be placed on the hot plate, the most likely artifact is that of empty
nuclei that will ruin the sections' usefulness.
René J.
--- On Fri, 8/13/10, Gibson, Philip philip.gib...@nuth.nhs.uk wrote:
From: Gibson,
I need some advice. We have been trying to do C4d on paraffin sections
without much success. Does anyone have a good protocol we could try?
We use the C4d from Quidel for our frozen sections and ideally I would
like a procedure that we can use with our Bond Max, but I may be hoping
for too much!
Totally agree with Rene. You will need to use some type of drying oven
Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Key Kim,
The blade angle depends on the manufacturer of the microtome. In general,
Leica suggests and angle of 3 to 5. Microm uses 10 to 12 and Shandon uses
0 to 3. Confused? Me too and I repair them for a living.
I have found that vibration is caused by problems with the knife holder
more often
We use the C4d from Cell Marque now with great results; it is an IVD.
ER1 20 minutes on the BondMax and Bond III
Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
We use the same antibody from Cell Marque on the Dako Autostainers with great
success.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From:
Hi Everyone
Just for clarification; our current practice involves a mixture of
placing racks of slides in an oven for 25 minutes, or using the hot area
of an autostainer (we're talking HE's here, BTW).
Occasionally, with a particularly urgent case us old-timers will place
individual slides
We have been using Dragon with SoftPath and works well, there's a little bit of
training the software at first but well worth it
Bill Lecorchick
Cytology Prep.Tech.
609-584-5128
Fax 609-584-6439
wleco...@rwjuhh.edu
www.rwjhamilton.orghttp://www.rwjhamilton.org
We use Dragon here as well.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu]
Hi Histonetters!,
How are you? I have a exciting new opportunity I want to tell you
about. I am working with a leading client located in the Sarasota area.
They are looking for someone to do Mohs histology. If you are
interested you would also be considered for management of the lab. You
What conferences or trade shows do you attend and find most valuable?
NSH? Pathology Informatics? Others?
Maggie Allen
Niceware International, LLC
Tel (810) 629-3930
Email: maggie.al...@nicewareintl.com
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Dear histonetters
i have a requist for all of you as i'm new in histochemistery and IHC as many
other frinds i know in histonet . we found it difficult to know the name and
use of many antibodies in many many topics so we can't understand the all
masages and replaing on it. and lose the
Mohamed,
Deposition of C4d is an inactive fragment of C4b (derived from complement
component C4). It provides in-situ evidence of complement activation following
alloantibody binding to graft vasculature.
As such, the development of antibodies against C4d has dramatically improved
the
Carol we are using Dragon with a Voice Brook interface. Dragon will
work by itself but has some issues with Pathology terminology. Voice
Brook was created specifically for Pathology. Our staff, PA's and
Pathologists really like this configuration.
Steve
-Original Message-
From:
In case anyone is still wondering, here is the response from CAP to a question
about their current position on use of RUO's for diagnostic use:
The CAP removed reference to RUOs in the checklist. The CAP position is RUOs
are not to be used for clinical testing.
Tim Morken
UCSF Pathology
San
I may have the chance to get a tissue processor (VIP 2000 or 3000). Are these
units still serviceable? Is it hard to find parts? Are they Reliable?
Thanks for any help!!
Mike
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I don't know the answer to your first 2 questions, but reliable they really are!
René J.
--- On Fri, 8/13/10, Mike Tighe mti...@trudeauinstitute.org wrote:
From: Mike Tighe mti...@trudeauinstitute.org
Subject: [Histonet] Tissue-Tek VIP 2000-3000
To: histonet@lists.utsouthwestern.edu
Date:
Agreed extremely reliable
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, August 13, 2010 3:41 PM
To: histonet@lists.utsouthwestern.edu; Mike Tighe
Subject: Re: [Histonet]
I am new to using a cryostat and am having some issues with my tissue.
1.Every slice seems to shatter and fragment. When I have used a
microtome it was because the tissue was too cold.
2. The tissue itself was flash frozen and so was not fixed and the tissue
did not go through
Joe the toe Nocito...are you out there?
Joe has good ideas about nails. Maybe he will send out his
procedure again.
I like using either potassium hydroxide 10-20% or Sodium
hydroxide 10-20% for softening nail fragments before
processing.
Also, keep in mind that soft tissues attached are
Happy Friday all,
try 10% sodium hydroxide for 1 hour prior to processing. It is important to
let the slides drain really well before placing them in the oven. You want
to make sure there is no water between the section and the slide. We use
Plus slides with plain distilled water in our water
I am a US trained and ASCP certified Histologist. I am looking for job
opportunity in Canada either as Histologist or as an IHC Specialist.
Thanking you all in advance.
Emmanuel.
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