Richard,
we do autopsies regularly at the weekends (both Saturday and Sunday) and also
metabolic cases as soon as we get the permit.
Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-Original Message-
From:
We are physically moved and Organized Chaos prevails (except in Histo,
of course!). There's been some discussion about what would be needed
when designing a new lab and I agree with what has been offered - and
actually used this sort of input from Histonet, along with the lab
design course at NSH
HISTONETTERS
I was trying my best to get out to Seattle for the workshop Steve was doing on
the lab design, our budget wouldn't allow it but any help I can get would be
great we are going to start construction Jan 2011 We are getting 3 patient
rooms and making them one big cytology/histology
I would love this same information.
Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR 72202
phone 501.364.4240
fax501.364.3155
visit us on the web at:www.archildrens.org
I would like this information also please.
Kathy, Gorham, H.T.
GRH National Recognition
Outstanding Rural Health Organization of 2009 awarded by NRHA
Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP
Leader in Innovative Excellence 2009 awarded by the OAHHS
Financial
Hi fellow histonetters,
I am trying to work out an assay for complement 3 and C5b-9 detection in
rodent. I have moved on to mouse, but have done some work with rats also. I
haven't felt all that successful in my endeavors. In mice, the tissue of
interest is kidney and have thus far
Nick Madary, HT/HTL(ASCP)QIHC
Medimmune Histology Mgr,
OMW, Area 4, Lab 2438
301.398.4745(vm)
301.398.6360(lab)
301.398.9745(fax)
To the extent this electronic communication or any of its attachments contain
information that is not in the public domain, such information is
Hazel-
Try calling Ventana. They were just in my lab (large reference lab in Houston)
and their evaluation system includes process and space evaluation and
suggestions. Barcoding speeds some areas and slows others down--nice to have
them come in and show you were to save a lot of time so
Hi Histonet's
I'm using Masson's trichrome to stain paraffin mice diaphragm fixed with
PFA.
I'm using this protocol:
http://www.bcm.edu/mcb/rosenlab/index.cfm?pmid=12997
with sigma masson's kit (#HT15) and Weigert's Iron Hematoxylin Set (#HT1079)
The staining is beautiful, but i can't see the
Hello,
A researcher wants me to do dimethyl methylene blue staining on some
cartilage sections. Is anyone familiar with this? Is it just another
name for standard methylene blue staining? Or is it actually another
dye? If so, where can I get the dye? And, does anyone have a staining
Hi Histonet's
I'm using Masson's trichrome to stain paraffin mice diaphragm fixed with
PFA.
I'm using this protocol:
http://www.bcm.edu/mcb/rosenlab/index.cfm?pmid=12997
http://www.bcm.edu/mcb/rosenlab/index.cfm?pmid=12997With sigma masson's
kit (#HT15) and Weigert's Iron Hematoxylin Set
I simply added the requirements for daily, weekly or monthly mainenance,
temp recording, cleaning under the procedure of using each piece of
equipment.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kathy
hello! Will you either provide me with info protocol for fixation of
adipose cryosections prior to staining and/or contact info for Dr. M.
Rocio Sierra-Honigmann ASAP? Thank you kindly, Atoska
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
Hello All,
I was wondering how you are filtering your cytology stains. We are a
relatively small lab, so we have been gravity filtering our cytology stains,
but as we are getting busier with a larger volume of slides, especially
fna's this is becoming a very time consuming process. I think
Hello all,
I have some questions for other Symphony users out there. Can someone please
contact me off-list if you don't mind answering some questions for me?
Thanks much!
Clare
Clare J. Thornton, HTL(ASCP),QIHC
Assistant Histology Supervisor
Dahl-Chase Diagnostic Services
417 State Street,
I have some questions about Fixing tissues for paraffin embedding when
the ultimate purpose will be looking for viral antigens and cell surface
markers.
We have some monkey tissue that has been in NBF for over a year. and we
are just now processing them and embedding them in to paraffin.
Itai,
Are you post fixing in Bouins? Post fix in heated Bouins for 1hr, wash well and
then follow your protocal.
Hope this helps,
Matt Lunetta HT (ASCP)
Longmont United Hospital
Message: 2
Date: Wed, 15 Sep 2010 18:27:29 +0200
From: Itai Moshe
Subject: [Histonet] Re: Problem with liver
Patrick:
Under separate cover I am sending an article about NBF fixation and its
comparison with other fixatives so you can make an informed decision.
René J.
--- On Wed, 9/15/10, Lewis, Patrick patrick.le...@seattlechildrens.org wrote:
From: Lewis, Patrick patrick.le...@seattlechildrens.org
Has anyone used the TDO66 Medite drying oven? Do you like it? Is the company
good to work with?
Margaret Perry HT(ASCP)
Dept of Veterinary and Biomedical services
Box 2175
South Dakota State University
Brookings SD 57007
605-688-5638
___
Histonet
HISTONETTERS
I was wondering what some of your thoughts on this.
Thanks
Pathology Supervisor
Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center
sbald...@mhhcc.org
Ph 812-482-0210, 482-0216, Fax 812-482-0232,
Pager 812-481-0897
Confidential information, Authorized use only.
I interpret this to mean that I, as the supervisor, need to document
monthly that temps have been recorded and instrument maintenance has
been recorded. I review and initial temp and maintenance charts at the
end of each month to make sure that it has been done and that temps are
in range.
Laurie
Laboratory Supervisor /Manager preferably with CLS Qualification
The Manager is a key role within the pathology organization located in Orange
County California. This individual will join our expanding laboratory
facilities, and will participate in daily operations of Histology, IHC, Flow,
Is there any way to relax curled brain sections? They've been in
cryoprotectant and I tried sitting them in PBS for a while. They're to be
used in an IHC- can they be rescued?
Thanks,
--
Teresa Iglesias
Graduate Group in Animal Behavior
University of California-Davis
Is there any way to relax curled brain sections? They've been in
cryoprotectant and I tried sitting them in PBS for a while. They're to be
used in an IHC- can they be rescued?
Thanks,
--
Teresa Iglesias
Graduate Group in Animal Behavior
University of California-Davis
Has anyone else noticed a change in the EM400 embedding medium from Surgipath?
We have not changed anything in our embedding, but now the EM400 results in
cracks in the blocks. Leica says although the labeling has changed to indicate
the EM400 is now also for infiltrating, the paraffin has not
Thanks, Montina
The sections are 40um and I had no trouble with all my other brains
(sectioned myself). I had help with these last two and they are all curled.
I think they just got sectioned too fast or something. I'll try the shaker
and see if it helps.
Thanks!
-Teresa
On Wed, Sep 15, 2010 at
Hello Histonetters,
I have been using Picrosirius Red stain to detect collagen fibers in
residues lifted from archaeological artefacts. The protocol has been
adapted for staining slides rather than tissue samples however I am
curious if it is normal that everything on the slide stains red. I
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