Good morning. I work part-time at a small lab and they have a Sakura Feintek
Tissue-Tek SCA tape coverslipper, model 4764.
I have not worked with any tape coverslippers before and am having problems
with areas of the tissue drying out. The solvent bottle is full of Xylene.
However, there is a
Please remove this email from the list.
sad...@hcmhcares.org
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*Sharon Adams*
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Are you letting the tape sit out (cure) for awhile befoe you use it? We leave
ours out overnight?
Lisa
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bales, Candy A
Sent: Tuesday, April 12, 2011 8:19 AM
Hello,
I see that the ASCO/CAP Guidelines recommend using amplified, cutoff,
and normal controls for Her2 FISH testing. We currently use Pathvysion
and purchase normal and cutoff controls from Abbott. They do not offer
a product of amplified controls. Are you all running this third
control? Are
Thank you all for your suggestions. I will observe the drip rate and increase
it as well as replacing the cracked lid on the solvent bottle. I did leave
the tape out for a couple of days before placing it on the machine as a friend
who has a different model suggested I do so.
Thank you again
Good morning,
I have been attempting to do some dual chromogenic staining. I am using
a mouse and a rabbit antibody. For the mouse antibody I am using an
HRP-mouse secondary followed by AEC for visualization. For the rabbit
antibody I am using a biotinylated rabbit secondary, followed by
Has anyone used the LabPulse microwave processor from EBS? We need a new
microwave for staining and retrieval and thought maybe we could get one that
will do processing also. I've looked at the Shurwave from Fisher, and the
Mars from Hacker. Are there any other companies that have small
That is a huge question. Here is a good place to start
NYS Clinical Laboratory TechnologySep 9, 2009 ... Clinical Laboratory
Technician. License Requirements ...
www.op.nysed.gov
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical
You could do that or you could use DAB for one antibody (brownish color),
followed by DAB + Nickel for the other (bluish color) and they are both
permanent and alcohol resistant.
René J.
--- On Tue, 4/12/11, Eva Permaul e...@georgetown.edu wrote:
From: Eva Permaul e...@georgetown.edu
Subject:
Milestone manufactures very good and versatile MW that process and stain.
René J.
--- On Tue, 4/12/11, Perry, Margaret margaret.pe...@sdstate.edu wrote:
From: Perry, Margaret margaret.pe...@sdstate.edu
Subject: [Histonet] microwave processors
To: histonet@lists.utsouthwestern.edu
We use inhouse tissue and made really tiny pieces to place them near the
specimen slide. So we bring it beneath one coverslip.
Bye
Gudrun
-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Kuhnla,
Today I made a further experiment. I did two titration runs. On one slide I
added the working solution by pipette on the other slide I added it by
pushing the filled PrepKit.
Both stainings came out beautiful. - There has to be something wrong with
the dispension-amount. Ventana is informed, I
Margret,
Which microwave would that be
THANK YOU,
PATTI RUBEN-NELSON H.T.(ASCP)
LABORATORY CONSULTANT
SUPERVISOR/DESERT GASTROENTEROLOGY CONSULTANTS
35-900 Bob Hope Drive
Suite 275
Rancho Mirage, Ca. 92270
cell (909) 841-9761 / wk (760) 321-2500
nelsonr...@verizon.net
Where can I find a procedure for dual staining of blood and bone marrow
smears using Vector anti-kappa and anti-lambda tagged with Coumarine
followed by FISH hybridization. I am able to see what I think are plasma
cells by their fluorescent blue cytoplasm and on the same slide (changing
filters) I
What is everyone using for their light when developing the silver in the
VonKossa stain when you have no sunlight to use? We used to use a 60 watt
lamp, but haven't done one for years and am bringing this stain back to our
repetiore due to pathologist request. Thanks much!
Dorothy Webb, HT
Funny you should ask... just last week I had two requests for a Von
Kossa. That's when I found out that, despite the fact that we just
moved into a brand new building with all the bells and whistles, there
was not one single UV light in any of the many hoods that had been
installed. Heaven
Date: Tue, 12 Apr 2011 11:37:17 -0400
From: Eva Permaul e...@georgetown.edu
Subject: [Histonet] Counterstain for dual chromogenic
To: histonet@lists.utsouthwestern.edu
Message-ID: 4da471ad.3040...@georgetown.edu
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Eva
I have been doing
WE use just a simple desk-lamp and put the coplin jar directly under the
bulb. And the desk-lamp is placed in the window.
Bye Gudrun
-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Webb,
Dorothy L
We have intense sun here but my windows face the wrong way! At least I have
windows.
I just use a light bulb from the lamp we have over the coverslipping station.
Works great.
Andi
On Apr 12, 2011, at 11:22 AM, Gudrun Lang wrote:
WE use just a simple desk-lamp and put the coplin jar
Hi,
Does anyone have experience embedding and staining in plastic? We have
rabbit rotator cuff tendons that are being embedded in plastic. I am trying
to figure out what the process is and which stains to use in order to
visualize (and quantify) blood vessels and collagen deposition at the Bone
Our lab is currently looking for a way to shorten our Biopsy processing program
without compromising the patient specimen. We do have an issue with our GI's
being very dry, which causes us to have to soak between each level taken and
also causes a lot of chatter. Also we have a goal to do a
Model KOS by Milestone is capable of tissue processing, decalcification,
special stains, fixation, gross hardening, and antigen retrieval.
René J.
--- On Tue, 4/12/11, SHANE NELSON nelsonr...@verizon.net wrote:
From: SHANE NELSON nelsonr...@verizon.net
Subject: [Histonet] Milestone MW
To:
You can use any strong intensity microscope light. The stronger the intensity
of the light the better, but the reduction has a cumulative effect so even not
very strong intensity bulbs can be effective.
René J.
--- On Tue, 4/12/11, Webb, Dorothy L dorothy.l.w...@healthpartners.com wrote:
I purchase a kit from Dorn and Hart Microedge and use sodium
carbonate-formaldehyde solution to chemically develop.
Stain in silver nitrate for 5 min in the dark, 3 fresh DI rinses for 1 min each
in the dark, then sodium carbonate-formaldehyde solution for 2 min in the dark,
2 fresh DI rinses
Try ethyl green:
1. 0.1N Acetic Acid
Add 6ml of glacial acetic acid to 1000 ml of distilled water.
2. 0.1N Sodium Acetate
Add 4.102 g of sodium acetate to 500 ml of distilled water
3. pH 4.2 buffer
Add 755 ml of 0.1N acetic acid to 264 ml of 0.1N Sodium Acetate and adjust
Interesting,
Meloan Puchtler (1985) have drawn our attention to Von Kossa's original work
on this technique. He regarded only the yellow colouration of calcium deposits
during early stages of the reaction as diagnostic for calcium phosphate and
credited the blackening to organic matter.
Increase the fixation time and make sure the air is out of the sponges (will
stop formalin from getting to the tissue- a quick dunk of the cassette (with
sponge and tissue) in alcohol and back into formalin will do the trick).
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC),
I would consider replacing the sponges. Tony is correct that you need to make
sure the air is removed from the sponge to facilitate exchange, but with
shortened processing times you will undoubtedly cause carry over from solution
to solution. Yuo may want to have a small container of alcoholic
I agree
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
Looking for a protocol to visualize vascularization and collagen deposition
at the bone-tendon interface of a rabbit rotator cuff embedded in a plastic
system. Sections will be rather thick (~50um) b/c they are being made
through a titanium anchor. Using MMA with a cold-curing resin, Technovit
From: eugenia90...@hotmail.com
To: histonet@lists.utsouthwestern.edu
Subject: 1 HE slide vs. 2
Date: Mon, 11 Apr 2011 13:06:59 -0400
Good afternoon everyone,
Does anyone know of an article or statistics discussing the impact (medical
diagnosing) of cutting 2 HE slides per block verses
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