[Histonet] Macrophage marker in sheep for IHC

Hi there, I am very new to basic sciences research and am planning an experiment to quantify and compare adipose tissue macrophage infiltration in sheep model using immunohistochemistry. I am specifically interested in CD11c as a marker of M1 activation of Macrophages. I have been advised that

Re: [Histonet] Frozen tissue question

From the research point of view, I've heard of people not fixing tissue before they section it. Since I work with embryonic tissue (which is mostly water!), we always fix our tissue. It definitely is better to not fix tissue when you want to stain with antibodies because anything you do to the

[Histonet] Tissue Shriveling in Paraffin

I was cutting some bone/joint tissue and noticed that the cartilaginous portion was concave/indented, instead of flush with the rest of the block surface. Even as I continued to cut that portion always seemed a little sunken into the block face and all the sections crumbled. I didn't seal the

[Histonet] SV-40 control blocks or slides

Hi, I am looking for a lab or a supplier for SV-40 control blocks or slides. If anyone can help us out it would be appreciated. Thanks Sharon Allen Senior Technologist Neuropathology Lab MS435U Health Sciences Centre 825 Sherbrook St. Winnipeg, Manitoba R3A 1R9 Ph# 787-4615 This email and/or

Re: [Histonet] Macrophage marker in sheep for IHC

CD68 - clone KP1 has not worked on sheep (also not dogs, cows, horses, and chickens) in my experience. I have found it to stain primates well (and rare cells in pigs and cats). Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota

Re: [Histonet] Tissue Shriveling in Paraffin

Why not try resin embedding techniques? Less shrinkage than paraffin, more specimen/block stability in cutting, no damaging effects of decalcification and a many times you have a better and more clear morphological representation. I could help you to achieve this if interested, so feel free to

Re: [Histonet] Sirius Red Stain

Why not save yourself some time and simply purchase the staining kit from Polysciences or Dorn and Hart Micredge. Jack On Jun 23, 2011, at 3:48 PM, Jesus Hernandez jesus.w@gmail.com wrote: Dear all, Does anyone have a protocol on how to prepare Sirius Red stain with known

[Histonet] Joplin's article

To those who were not able to open to the site, I tried to copy and paste and hopefully you can read it. This is a very interesting story about Joplin's St John's Regional Medical Center and code Gray. If you still have problems. please visit this link.

AW: [Histonet] Tissue Shriveling in Paraffin

That sounds like insufficient infiltration with paraffin. Melt your block again and let it sit in paraffin for a few hours or even over night. Most time this helps to get better sections. Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu

[Histonet] Looking for 2-3 week temp Histology assignment in July

Hi Histonetters! I am looking for a temp histology assignment for a couple of weeks in July. I am HT (ascp) and have 17 years experience. Please respond to this email if you are looking for some help, thank you, Jill Jill Cox, HT ASCP ___ Histonet

[Histonet] Invitation to connect on LinkedIn

LinkedIn Adekunle Oluwatosin Adeluwoye requested to add you as a connection on LinkedIn: -- David, I'd like to add you to my professional network on LinkedIn. - Adekunle Oluwatosin Accept invitation from Adekunle Oluwatosin

[Histonet] Invitation to connect on LinkedIn

LinkedIn Adekunle Oluwatosin Adeluwoye requested to add you as a connection on LinkedIn: -- David, I'd like to add you to my professional network on LinkedIn. - Adekunle Oluwatosin Accept invitation from Adekunle Oluwatosin

Re: [Histonet] Formula R paraffin

If sometimes you are experiencing compression problems it could be because your paraffin has some xylene contamination, or your blocks are not cold enough when cutting, or you are using a paraffin of lower melting point as needed for the tissue you regularly process. Paraplast X-tra always

[Histonet] HistoGel

Hello, Does anyone out there have any experience with HistoGel? It's Richard Allan/Thermo Fisher. They claim that you can embed scant tissues in the gel and then process, embed, and cut as usual. Just wondering how it works in the real world Michael J. Dessoye, M.S. | Histology

[Histonet] (no subject)

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[Histonet] RE: HistoGel

We use histogel a lot in our lab. It's a research lab and we use it for a few purposes - pelleting cultured cells then creating multi-culture TMAs for testing antibodies and also pelleting cells from ascites and pleural effusions. Has also been used to process really small samples that could

RE: [Histonet] Histogel Problem

Hi, I've encountered this problem before in my previous lab. To reduce the Histogel from shrinking that badly, avoid putting the tissue at the very edge of the Histogel, space them out nicely in the middle, providing sufficient amount of extra Histogel between each tissue and surrounding them.

[Histonet] bluing

Looking to change my bluing step in the HE process to obtain a bluer (less purple) hue to the nuclear detail. What is everyone using in their bluing step?? Thanks for all of your ideas!! This e-mail and any files transmitted with it are confidential and

Re: [Histonet] bluing

There are several bluing solutions in the market, or you could use lithium carbonate at different concentrations until you find one of your liking. René J. --- On Fri, 6/24/11, Webb, Dorothy L dorothy.l.w...@healthpartners.com wrote: From: Webb, Dorothy L dorothy.l.w...@healthpartners.com

RE: [Histonet] bluing

Hi Dorothy, Try Richard Allan Bluing Reagent. Here's what they say about their product: It is a buffered product that ensures the proper alkalinity (pH=8.0). Unlike ammonia and lithium carbonate, RA's Bluing Reagent does not allow for the pH shift which can affect the crispness of nuclear

[Histonet] blades

Trying to clean up some things hanging out there in our lab and wondering what everyone does with a blade that has been used minimally and tech done for the day with the microtome. Where do you store that blade for use tomorrow or do you toss and not worry about the cost involved? I do not

Re: [Histonet] blades

Dorothy, I put ours in a 15 mL centrifuge tube with a cap sit it on the base of the microtome for the next use, that way, no one gets cut the blade is able to be used to the fullest of it's potential. :-) Best regards, ~Sean McBride Scientific Specialist Bone Tissue Engineering Center

Re: [Histonet] blades

I use the cardboards that come in a box of slides. A small piece of tape on the open side and mark it used. I just always make sure I have the blade edge facing the folded part. I know some who will tape this folded board it to the side of their microtome and use it as a trimming blade holder.

Re: [Histonet] blades

We use plastic 5 slide mailers Sent from my iPhone On Jun 24, 2011, at 1:53 PM, Webb, Dorothy L dorothy.l.w...@healthpartners.com wrote: Trying to clean up some things hanging out there in our lab and wondering what everyone does with a blade that has been used minimally and tech done for

[Histonet] HT Position - Irvine, CA

Hi Histonet, We are currently looking for a full-time Histotechnician (preferably HT certified) with experience in IHC. Please read the description below and if you're interested please submit your resume via e-mail to: * eric.velazq...@agendia.com*. *ESSENTIAL DUTIES AND RESPONSIBILITIES*