RE: [Histonet] interview

2013-01-09 Thread Edwards, Richard E.
Well if you only pay peanuts you only get monkeys.. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: 09 January 2013 00:05 To: Gale Limron; histonet

[Histonet] RELIA Special Job Alert - Exciting opportunity in Philadelphia for a Molecular Diagnostic Tech 01/09/2013

2013-01-09 Thread Pam Barker
Hi Histonetters!! I hope everybody is having a great day. I have a new position to tell you about that I am pretty excited about. If you are interested please let me know and if you know someone who is interested please pass the info along. Remember if I hire someone you refer you get a

[Histonet] RE: Control Tissue

2013-01-09 Thread Connolly, Brett M
Amy - here are a few... Cooperative Human Tissue Network- http://www.chtn.nci.nih.gov/ Asterand biorepository. - https://www.asterand.com/Asterand/ NDRI - http://ndriresource.org/ Analytical Biological Services, Inc. - http://www.absbioreagents.com/products/products.html Brett M.

Re: [Histonet] Control Tissue

2013-01-09 Thread Rene J Buesa
At the start of your lab activities you will have to rely on colleagues from other labs willing to give you old blocks (those not required to be kept any longer) that are positive for some procedures. With time you will build your own collection from your cases.  Try always to use tissues that

Re: [Histonet] Histology growth/volume ??

2013-01-09 Thread Rene J Buesa
Check CAP Today. This is a monthly publication from CAP that deals with this type of issues. Try to contact them with your questions. René J. From: Kiranjit Grewal kira...@sbcglobal.net To: Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu; Richard Cartun rcar...@harthosp.org;

[Histonet] RE: Microtome blades

2013-01-09 Thread Bernice Frederick
VWR Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From:

[Histonet] Microtome blades

2013-01-09 Thread Laurie Colbert
Does anyone know who I can purchase Accu-edge blades from, other than Cardinal or American Master Tech? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab ___

[Histonet] RE: Microtome blades

2013-01-09 Thread Boyd, Debbie M
Mercedes Medical -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Wednesday, January 09, 2013 10:56 AM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet]

[Histonet] RE: Microtome blades

2013-01-09 Thread Elizabeth Chlipala
Mercedes Medical or directly from Sakura Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO

Re: [Histonet] Microtome blades

2013-01-09 Thread Rene J Buesa
Sakura René J. From: Laurie Colbert lcolb...@pathmdlabs.com To: Histonet Post (histonet@lists.utsouthwestern.edu) histonet@lists.utsouthwestern.edu Sent: Wednesday, January 9, 2013 10:55 AM Subject: [Histonet] Microtome blades Does anyone know who I can purchase Accu-edge blades from, other

RE: [Histonet] Microtome blades

2013-01-09 Thread Bartlett, Jeanine (CDC/OID/NCEZID)
If you order them from Cardinal they get them from SakuraI think. Or maybe vice-versa... Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From:

[Histonet] Re: Histonet Digest, Vol 110, Issue 9

2013-01-09 Thread Madeleine Huey
Accu-edge microtome blades from Fisher Scientific. madelein...@elcaminohospital.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Wednesday, January 09, 2013 9:56 AM To:

[Histonet] Re: Histonet Digest, Vol 110, Issue 9

2013-01-09 Thread Madeleine Huey
Hello Gale, I normally ask the candidates questions relating to embedding/cutting/special stains. For example Special Stains; - how do they perform a test for collagen and smooth muscle (answer - Special Stain Trichrome). If they answer correctly with Trichrome, then ask them WHY Trichrome

RE: [Histonet] interview

2013-01-09 Thread joelle weaver
Yes, please interview and hire people with experience and/or training! The situation in histology will never get better otherwise. Joelle Weaver MAOM, HTL (ASCP) QIHC From: r...@leicester.ac.uk To: wdesalvo@outlook.com; ga...@unionhospital.org; histonet@lists.utsouthwestern.edu

[Histonet] Position Available- San Francisco Bay Area

2013-01-09 Thread Heidi Hawthorne
On Assignment Healthcare is currently looking for a Histotech to work in an acute care facility in the San Francisco Bay Area. This position is Monday-Friday 5:30am-1:30pm (part time availability may be considered). The position will require paraffin embedding, grossing, cutting, and fixing

[Histonet] Call for Speakers

2013-01-09 Thread Jennifer MacDonald
The California Society for Histotechnology is holding their annual symposium May 3-5 at the beautiful Hilton San Francisco Airport Bay Front Hotel. We are looking for speakers. If you are interested in speaking please contact me. Thank you, Jennifer MacDonald CSH Secretary

Re: [Histonet] interview

2013-01-09 Thread Pam Marcum
I totally understand hiring only experienced people however; I have a question.  What do you do when you have no one available and the institute you work for will not help with moving expenses or sign on bonuses?  Believe me I know about training OJT today when you are shortstaffed and

[Histonet] Reagent Alc or Denatured Alc ?

2013-01-09 Thread Stella Mireles
What is the difference between the two ? Thinking about switching to denatured becasue of cost costing. Which do you prefer? Stella ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Xylene Free Processing?

2013-01-09 Thread Jones, Laura
I would appreciate hearing all of your expert opinions on processing tissue without the use of xylene or any type of xylene substitute. Our processor was down recently, and a friendly local lab processed our tissue this way for us. Of course, the Pathologists loved it. The process was

RE: [Histonet] Reagent Alc or Denatured Alc ?

2013-01-09 Thread Walter Benton
They are one in the same basically, meaning not fit for human consumption, by means of adding methanol or other impurity. Pure ethanol would require an alcohol license and inventory tracking since it is fit for human consumption. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake

Re: [Histonet] interview

2013-01-09 Thread Rob Geske
Hi Pam, if there is no one qualified locally i would suggest you look to a temp agency that does have qualified individuals that can provide the skill sets you require.  if someone needs to be brought in from outside your area, the agency will certainly charge you per diem in addition to

[Histonet] Immunohistochemistry

2013-01-09 Thread Allyse Mazzarelli
To everyone in histoland that is familiar with IHC, I am a new histologist, unfamiliar with many techniques in IHC. Here in my lab, we section devices that contain cells, membrane and scaffolding. Unfortunately, due to the scaffolding, the devices do not hold up well when embedded in paraffin.

[Histonet] RE: Microtome blades

2013-01-09 Thread Hugh Luk
Laurie, Accuedge can also be found through: VWR (https://us.vwr.com/store/catalog/product.jsp?catalog_number=25608-964) or Electron Microscopy Services http://www.emsdiasum.com/microscopy/products/histology/sectioning.aspx I believe we're in a similar situation, as our institution has Favored

RE: [Histonet] Xylene Free Processing?

2013-01-09 Thread O'Donnell, Bill
The Xpress microwave tx processors are xylene free - perhaps they have one. I am pretty certain there still needs to be something that is misable with alcohol and paraffin, such as mineral oil. I believe this can be added to one or two of the paraffin steps, but I am just venturing a guess. Most

Re: [Histonet] Xylene Free Processing?

2013-01-09 Thread Rene J Buesa
Please go to http://www.histosearch.com/rene.html and you will find my articles on the sibject. René J. From: Jones, Laura lpjo...@srhs-pa.org To: Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu Sent: Wednesday, January 9, 2013 2:37 PM Subject: [Histonet] Xylene Free

Re: [Histonet] Xylene Free Processing?

2013-01-09 Thread Rene J Buesa
A mixture of isopropyl alcohol and mineral oil is the best solution to complete a gentle and graded tissue processing. To clean the tissue processor you can use a 5% solution of dishwasher soap in isopropyl alcohol. You can find my article on the subject in http://www.histosearch.com/rene.html

RE: [Histonet] Xylene Free Processing?

2013-01-09 Thread Sarah Dysart
FYI, I've tried ClearRite, and while I guess it technically works...it's a giant pain to work with because you have to extend out your times. I also found that it screws with Eosin because if it gets moisture in it (which you can't see); the water bleaches out the Eosin. Just my two cents

[Histonet] RE: Histonet Digest, Vol 110, Issue 9

2013-01-09 Thread Mayer,Toysha N
Amy, Try contacting the local hospitals for some blocks. For trichrome and mucin, you can use small intestine. Those are usually readily available from a local facility. Iron uses liver or spleen and those can be obtained the same way. By networking with other facilities you can begin a

RE: [Histonet] interview

2013-01-09 Thread joelle weaver
Yes, I understand that it can be a difficult situation. I just feel that so long as it continues as it has, we are likely to get the same result. If you have no applicants who are trained or experienced, and you cannot lure someone with experience and training by incentives, then I feel you

[Histonet] Re: Reagent alcohol or denatured alcohol

2013-01-09 Thread Bob Richmond
Stella Mireles asks: Reagent Alcohol or Denatured Alcohol? What is the difference between the two? Thinking about switching to denatured because of cost costing. Which do you prefer? Reagent alcohol is a commonly used term for ethanol (ethyl alcohol) to which 5% methanol and 5% isopropanol have

RE: [Histonet] Xylene Free Processing?

2013-01-09 Thread Finley, Sue [PH]
We use xylene free processing + microwave Formalin, 100% ethanol, isopropanol (clearant) and paraffin and have been doing so for 4 years -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill

Re: [Histonet] interview

2013-01-09 Thread Pam Marcum
In the 45 plus years I have been in Histology I seen this many times also.  Unfortunately, we are still the unheard of area of the lab.  T his is not changing through any of the organizations who are happy to take our money and not help us get out who we are and what we do.  I have

Re: [Histonet] RE: Microtome blades

2013-01-09 Thread Stella Mireles
I purchase Accu-edge blades from Stat Lab. Thanks to All for the comments on reagent and denatured alcohol. On Wed, Jan 9, 2013 at 2:00 PM, Hugh Luk hlu...@msn.com wrote: Laurie, Accuedge can also be found through: VWR