This could be as simple as moving the specimen holder forward. It may be racked
right into the microtome and therefore show as stopped.
Try moving the holder forward and see if the stopped light goes off.
Regards
steve weston
lab manager
Breathe-Well CRE
UTAS-SOM
Message: 9
Date: Sat, 15
Ever tried turning the handle of the cryostat, when it's at room
temperature?
Cryostats are tooled manufactured to operate at a low temperature.
Since metal contracts at the low temperature, you'll find that you can't
operate the microtome at the higher temperature. The handle will barely
move.
You might also consider using methyl salicylate instead of xylenes. Thanks to
the help of Bob Skinner, I have achieved very nice results with native tendon.
Generally speaking these MS steps will take a little longer, but you can
monitor the progress very easily by watching for complete
Hi All,
Hope all are having a great Monday
I am trying to do a Picro Sirius Red stain and have looked at many protocols
that seem to not use a particular reagent that is in a manufacturers kit. I was
hoping if someone could enlighten me as to why this reagent would be used and
if someone
Hello Histonet! I'm a long time reader first time poster. Does anyone have
experience processing guinea pig tissues? I have been processing kidney and
heart but it is consistently coming out mushy in the middle. The mouse
tissue comes out fine even when processed on the same run. I had the tissue
I do a Picro Sirius Red stain for collagen (got the protocol from Gayle Callis)
and don't use Phosphomolybdic acid.
My protocol is simple:
one hour in PSR
two rinses in acidified water
rinse
you can counterstain but my clients don't want a counterstain so I dehydrate,
clear and coverslip.
Boom,
Good Morning,
Can anyone recomend a good, reliable cryostat repair service in the San diego
area?
thanks
Dusko
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Dear John,
I usually perform Picrosirius Red stain in cardiac tisse sections.
I use phosphomolibic acid treatment in order to eliminate the cytoplasmic
staining improving collagen/myoplasm contrast.
Have a look at this paper:
Picrosirius red staining of cardiac muscle following phosphomolybdic
Does anyone have an ACIS Calibration set they are not using and are willing to
part with? One of my slides broke and I can't find a replacement anywhere.
Thanks,
Kari Breal
Kari Breal, HT (ASCP)
Histology Manager
Alexian Brothers Health System
ABMC-847-437-5500 ext. 5155
SAMC-847-843-2000
Heather,
Looking at your protocol for processing the kidney and heart tissue I can't
figure out why it is mushy especially if you are putting it in the cassettes so
thin and it has had a chance to fix well before processing. In fact, looking at
your protocol I might think that your tissue might
Hello all you Histologists,
Does anyone know of a used Histology equipment company that
sells Sakura parts? We have a fairly new embedding center, (but past warranty),
that needs a single heater unit. Sakura only sells the entire dispenser unit
for nearly $8,000. I don't mean
Hi Histonetters,
I am moving back to Phoenix, Arizona and I am looking for a Histology position.
I have over 17 years experience in the Histology field. I am willing to work
full time and even part time. Please feel free to contact me at
kecsl...@yahoo.com I hope to hear from you soon.
I work in a hospital, there are three of us on this particular shift and we
cut approx. 200 blocks, give or take a few. Our histo lab manager is
telling us we should only be using one pack of blades (50 per pack) a
month. I'm wondering what other techs think of this especially lab
managers and
That's penny pinching right there. I'd say fine, you tell me when to change
blades and then put through the crap slides they get. Just avoid the calculi
and staples at all costs... sometimes these number crunchers don't think.
Curt
-Original Message-
From:
Sorry, I tell my techs a knife is cheaper than a complaint from the
pathologists so, please don't abuse. H owever; don't hesitate to use what you
need. As teh old saying oges for us A happy Patholgist is a happy life!!
A dull is bad for sectioning and is dangerous overall. I can't
From a managers point of view, whoin my opinion that is a poor way to try to
cut expenses. It will only lead to recuts and possible loss of important
tissue. For the techs to understand the necessity to conserve is important but
the tech needs to use their discretion as to when a blade needs
We are in the process of updating job descriptions and want to use this
opportunity to see how other organizations are handling the difference in job
descriptions between HT's and HTL's. Obviously if you have a different pay
schedule for the two positions you need to clearly delineate the job
St. Anthony Hospital, located in Oklahoma City, Oklahoma currently has an
excellent opportunities for experienced Histologic Technicians. To work for our
organization requires Certification as an HT or HLT by the American Society of
Clinical Pathologists (ASCP) - or - other nationally
We have Histotechnologst 1, 2, 3, and Lead, Supervisor: We don't have a
requirement that they have their HT or HTL certification, but do need specific
experience in a histology lab.
All require bachelor's degree with educational qualifications to sit for HT or
HTL exam. Most have their HT or
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