We have had pads replaced as well but the problem continues.
Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
I am sending this on behalf of our Director. Thanks in advance for any and all
responses. Tony Boris
I am the Medical Director of Surgical Pathology at St. Joseph Mercy Hospital
Oakland, located in Pontiac MI. I staff our weekly breast cancer treatment
planning conference and am responsible
We are also having similar issues. Our instruments are about five years
old. I wonder if at a certain point they just start to fade out..?..
Very interesting to see the issue here on histonet. Must be more common
that we realize.
Melissa Kuhnla
Lead Technologist for IHC and ISH
Catholic Health
For anyone who grosses prostate needle biopsies- does anyone place 2 sites in
one cassette , marking one site with ink to identify the site? I would
appreciate any opinions or ideas on this technique.
Debbie Granato HT(ASCP)
Sent from my iPhone
Oppenheimer Urology Lab does that. I don't like the idea, but I guess it
is a cost savings. We do not do it here.
Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0230
-Original
Hi Histonetters! We are looking for a part time tech here in our very busy
Delray Derm Lab. This is a permanent part time position with full time growth
potential. PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES!!!
Email your resume to ksime...@leavittmgt.com if interested.
*part time position
I agree that the best you will probably find is someone with certification in
one discipline that will be willing to train in the other. I am a CT and HTL
and even though the fields are so closely related, people like me with both
certs (and experience) are very few.
Brian Gaylord CT, HTL
Jim Vickroy in US area code 217 asks for ASR antibodies verses IVD
antibodies so here are some verses for the occasion. (Happy Friday Eve!)
What's an ASR?
What's an IVD?
(I do know FDA
and sometimes CAP.)
Endless abbreviations
leave me with frustrations.
Please, spell it out,
for this aging lout!
I'm going to try sectioning at higher temps today and I hope that it works.
Thanks all for the suggestions.
We used to have a vibratome but the thing only worked right for certain kinds
of samples. I don't see us getting another one since the occasion to use
something like that happens very
Dr. Richmond,
I frequently share your frustration with acronyms and abbreviations, but
these are two that I actually know. ASR is Analyte Specific Reagent, a term
that is applied to many of the monoclonal antibodies that we use in the
clinical immunology lab, and also to antibodies used in
Hi Andi,
I second the option for Vibrotome section. I think that will be your best bet
and should provide the least amount of artifact.
If you must use the cryostat, I agree that -18 is way too cold, go warmer than
you are ordinarily comfortable with. If the OCT can't handle it, you might try
I wish I could do free floating sections! These are mounted on a slide.
8-(
Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724
1) Does anybody use a Texas Red filter for viewing IHC slides stained
with Sox10, red chromagen? I have used several different co.'s red
chromagen. We get positive staining. However, if we get as vibrant as my
pathologist likes then there is a slight blush. He returned from a meeting
Dear Mary Ann,
I'll (hopefully) beat Gayle Callis to the punch on red chromogen/fluorescent
detection. According to her experience, you need to slightly understain the red
chromogen to achieve the best fluorescent signal. I don't know if it has to do
with saturation but too much red deposition
Hi
I often section brain sections at 50 microns. However, I use a sliding
microtome. For me, those are the best at sectioning thick brain sections.
Vibratomes are great too. I would use the cryostat as a last resort. I also
agree that if it is too cold, it wont section correctly and will
Does anyone know of a reference lab that performs staining or any
testing on tissue for Granulocyte Colony Stimulating Factor? I have
found labs that perform the testing on blood, but haven't been able to
locate one yet that does this testing on tissue.
Thank you!
Connie Dieringer, HTL (ASCP)
Our hospital has not allowed freezing spray to be used in the frozen section
lab for many years. We now have a new group of doctors who want to use the
spray. The docs think the frozen sections take too long to freeze. Yet, they
meet the frozen section TAT for more than 98% of our cases. I
If they are hospital employees, you should have a little more say in what goes
on in the lab. Is there a policy which addresses this? If not, now might be a
good opportunity to draft one and have the Medical Director sign off on it. You
can also get your Infection Control department involved if
Mark
Sounds like I will be in similar circumstance soon. I am not a molecular person
and I am not certified in molecular OR cytogenetics. Just HTL/QIHC, I do have
degrees but it never made that much difference really so far. I have been
trained in FISH and FISH enumeration (barely- just
Reasons against the use of freezing spray for frozen sections:
From the Federal Registry 1910.1030: “All procedures involving blood or
other potential infectious materials shall be performed in such a manner
as to minimize splashing, spraying, spattering, or generation of droplets
of these
Hello all :-)
I am doing a quick microtome blade safety survey,
When you finish work, do you leave your blade in the microtome behind the blade
guard or do you take it out?
If you take it out and it is a new blade or a blade still useful for trimming
where do you store it?
Thank you for your
I gross
prostate needle biopsies every week.
I receive
12 samples but convert them in 6 paraffin blocks.
I stain
the Medial Samples of each level and each side with Eosin and the Lateral
Samples with EA - 36 .Example: Base Lateral Right ( EA - 36 ), with Base Medial
Right ( Eosin ).Base
We save the blade to use for trimming. We store the blade in a plastic
5-slide mailer.
From: Leah Simmons leah_simmon...@hotmail.com
To: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Date: 10/24/2013 06:36 PM
Subject:[Histonet] Microtome Blade safety, in
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