Hi Charles,
I have had excellent success with lysing the red blood cells (using Isotonic
Ammonium Chloride) prior to cell block preparation with thromboplastin-plasma.
The lysing solution contains EDTA so you will need to add a few drops of 1%
calcium chloride. Method as follows:
Lysis soluti
I agree with Joe. We used to use ETOH for cell blocks, but stopped using it
when we started doing IHC biomarker testing on these specimens. Alcohol is
good for some proteomic targets, but can be a disaster for others. We also fix
all of our cell block specimens that are collected in saline or
Are the tissues taken directly from the molten wax and placed in the
wax-containing mold or are they allowed to cool before embedding?
One cause of tissue separation is the difference in temperature between tissue
and embedding wax.
Try taking directly from molten wax and embedding directly in
As a cytotech, that wouldn’t be my first choice for collections and FNA
specimens. The main reason is that once fixed in 95% ETOH you are limited if
you need to perform IHC stains on the cell block unless you have validated your
IHCs on ETOH fixed specimens. How do you process the FNA rinses t
Katherine:Our lab did the same thing a few years ago and I received operating
instructions for this system from a sales rep with Biocare that I know. I'll
send this document to you offline. I can also put you in touch with the guy
from Biocare. As Colleen said, Biocare doesn't sell or support
Our tech said they use 95% alcohol to collect the specimen.
On Fri, Oct 25, 2019 at 12:23 PM Joe W. Walker, Jr.
wrote:
> Hi Charles,
>
> What are you collecting the FNA into? Cytorich? Cytolyt? Other?
>
> Joe W. Walker, Jr. MS, SCT(ASCP)
> Anatomical Pathology Manager
> joewal...@rrmc.org, www.
Hi Charles,
What are you collecting the FNA into? Cytorich? Cytolyt? Other?
Joe W. Walker, Jr. MS, SCT(ASCP)
Anatomical Pathology Manager
joewal...@rrmc.org, www.rrmc.org
-Original Message-
From: Charles Riley via Histonet
Sent: Friday, October 25, 2019 8:13 AM
To: Histo List
Subject:
Hi,
When I place my sections on the waterbath the paraffin pulls away from it.
Leaving just the edge of the tissue. It is very weird. I have tried at
different temps . The tissue itself is fine so I do not believe it is the
processing. I use the same paraffin for processing as well as embedding.
Where does everyone purchase their IFW from? or how do you produce it in
house?
--
Charles Riley BS HT, HTL(ASCP)CM
Histopathology Coordinator/ Mohs
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Does anyone have any tips or suggestions on how to better process extremely
bloody FNA specimens?Is there anyway to clear out some or all of the
blood without destroying the other tissues?
--
Charles Riley BS HT, HTL(ASCP)CM
Histopathology Coordinator/ Mohs
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