Re: [Histonet] ER/PR question

The antigen doesn't decay much inside the tissue block, as long as the sections were freshly done. We redo ER/PR on archived samples from 15 years ago and they still stain very strongly. Allan On Thu, Feb 7, 2019 at 8:26 AM Heckford, Karen - SMMC-SF via Histonet <

Re: [Histonet] Clear-Rite 3 dirty slides

Thanks all, looks like the consensus is that water into the clearing agent causes it and I should change out alcohol more frequently. Allan On Wed, Oct 31, 2018 at 5:46 PM Allan Wang wrote: > Hi histonet, > > For coverslipping Ventana IHC slides I have dish soap, rinse, alcohol 80%, > 100%,

[Histonet] Clear-Rite 3 dirty slides

Hi histonet, For coverslipping Ventana IHC slides I have dish soap, rinse, alcohol 80%, 100%, 100%, 100%, followed by three Clear-Rite 3 instead of xylene. In the past I didn't have this issue, but now I frequently get a lot of residue on the slides after they enter the Clear-Rite. If I

Re: [Histonet] benchtop fume hoods

I'd like to know as well. Thanks, Allan On Thu, May 10, 2018 at 11:25 AM, Histology via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi all, > > Can anyone share what type of benchtop fume hoods they are using in the > lab? This would be for manual coverslipping and grossing. > >

Re: [Histonet] effect of power outage on tissue processor

A generator is probably the cheapest option if you can go manually start it after a few hours. I purchased a UPS for a DNA sequencer which shouldn't lose power when in use. You may also want one to add a few hours of leeway before the generator is needed. You should measure the tissue processor's

Re: [Histonet] IHC validation

How are labs validating for rarer biomarkers like ALK, ROS1, or MMR loss? Following the guideline of 10 positive cases may be difficult. I've seen other companies with control slides just with a few engineered cell lines as positive and negative controls. Is that enough for validation alone? We

Re: [Histonet] MMR Negative controls

Hi, We (at US Biomax) recently screened our colon TMAs for loss of expression for MLH1, MSH2, MSH6, PMS2 using Ventana's antibodies. If you need around 10 cases of negative tissue: CO20813a, 208 cases of colon, contains loss of: PMS2: 35 MLH1: 18 MSH6: 14 MSH2: 8 This TMA is not listed on our

Re: [Histonet] Decontamination Of Ventana Benchmark Ultra

Lysol IC as recommended by the tech and manual. I just buy the concentrated one on Amazon and dilute it 1:250 Allan On Mon, Jan 22, 2018 at 8:27 AM, Sharon Dwyer via Histonet < histonet@lists.utsouthwestern.edu> wrote: > What solution are you using to do the decontamination of the Ventana >

Re: [Histonet] mounting medium

Hi, This information would be very useful for me and probably others as well, since I've just arbitrarily chosen one. Can you summarize the responses you received for us? Allan On Wed, Nov 8, 2017 at 10:28 AM, warda hassan via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hello to all

Re: [Histonet] benchmark ultra bulks

The LCS is fairly cheap in comparison to some other bulks and 650-010 is half the price of 650-210. I don't know if there's actually any difference between them. I think an alternate formulation for EZ Prep (or Discovery Wash), CC1 and especially CC2 would save more money. Allan On Fri, Nov 3,

Re: [Histonet] help!!

Tim and Tony, Why couldn't DAB be used on frozen sections in your example? Allan On Mon, Apr 17, 2017 at 9:03 PM, Tony Henwood (SCHN) via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi Bianca, > Well for most Pathology departments, Immunofluorescence (IF) is used for > Renal and

Re: [Histonet] ventana customers

I've found that using Clear Rite 3 instead of xylene prevented the stickers from peeling at the bottom and producing that sticky residue. But I also have the liquid level low to prevent direct contact. Allan Wang On Tue, Jan 24, 2017 at 8:53 AM, Duddey, Aimee via Histonet <

Re: [Histonet] Dirty H after break

dney > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > -Original Message- > From: Allan Wang via Histone

[Histonet] Dirty H after break

Hello all, Does someone know what would cause dirty flakes of light purple that showed across the whole slide? http://i.imgur.com/1nIXwAy.jpg Reagents are relatively fresh but the H stainer hadn't been used for a week over the holiday break. Is it surface film on the hematoxylin? I didn't

[Histonet] Adding glycerol to concentrated antibodies

Hello all, I noticed CST sells most of their antibodies with 50% glycerol and recommends -20C storage and no aliquoting. They remain in liquid form in the freezer. A little bit more difficult to pipette precisely due to the viscosity and temp difference, but it seems like a good trade-off for

[Histonet] Histotech opening in Rockville, MD. Immediate

Hello list, I'm looking for a histotech with 2-4 years of experience, starting immediately. Position is at a small business which is a biorepository for cancer researchers worldwide. Job responsibilities: 1. Section, mount and stain very valuable tissue blocks. No tissue processing for now. 2.

[Histonet] RTU antibodies with 1+ year expiration date

Hello list, Thanks for your responses to my previous dehydration question. I pretty much got conflicting responses about going straight to 100% alcohol, but I think I will just start them out in 80% alcohol to be safe. Something that's been bugging me: Every source states that diluted antibodies