Re: [Histonet] Agarose embedded tissue arrays embedded in paraffin block

Exactly, I process the agar,not the tissue in it Cells,organoids, spheroids. Bernice -Original Message- From: Colleen Forster via Histonet Sent: Friday, May 17, 2024 3:07 PM To: Jay Lundgren Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Agarose embedded tissue arrays

[Histonet] FW: Golgi-cox staining

From: Rizaldy P Scott Sent: Wednesday, April 10, 2024 4:33 PM To: Bernice Frederick Subject: Re: [Histonet] Golgi-cox staining Hi Bernice, What they should have done ideally is to store the uncut agarose blocks submerged in whatever buffer they used to rinse the brains out prior to agarose

Re: [Histonet] preordering giemsa stains

We used to Alcian Yellow... Bernice -Original Message- From: Gudrun Lang via Histonet Sent: Thursday, February 29, 2024 8:48 AM To: 'Paula' Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] preordering giemsa stains In my place it is ordered on demand, but the pathologists

Re: [Histonet] Von Kossa staining

I use a 60 watt bulb and it works fine. Bernice -Original Message- From: John Kiernan via Histonet Sent: Wednesday, July 26, 2023 11:51 PM To: Charles Riley ; histonet@lists.utsouthwestern.edu; dsiena Subject: Re: [Histonet] Von Kossa staining Charles, A handheld light of any kind i

[Histonet] water under sectios

l. Bernice Bernice Frederick Pathology Core Facility Robert H. Lurie Cancer Center 710 North Fairbanks Court Olson 8-421 312-503-3723 b-freder...@northwestern.edu<mailto:b-freder...@northwestern.edu> ___ Histonet mailing list Histonet@lists.utsouth

[Histonet] RAt teeth

Hi all, I have bamboo rat teeth that have (ha-ha) been decalled. Obviously they are not soft. Can I use Nair on them or is there something better? I will need an H&E and an iron stain. They are not tiny samples.. Bernice Bernice Frederick Pathology Core Facility Robert H. Lurie Ca

[Histonet] P16

Who is the best and current supplier for p16? We were using biocare,but evidently it is no longer available from them. And no Santa Cruz per my IHC tech. Thanks, Bernice Bernice Frederick Pathology Core Facility Robert H. Lurie Cancer Center 710 North Fairbanks Court Olson 8-421 312-503-3723 b

Re: [Histonet] sectioning issues?

histonet@lists.utsouthwestern.edu> for histonet. It is a forum. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu<m

[Histonet] TUNEL

reagent in the stain be causing it? DAPI is used... Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu<mailto:b-fre

[Histonet] Fibroblasts

Hello all, Don't have a ton of time to search so am asking. Any good markers specific to fibroblasts? And am drawing a blank for regular special stains for some reason. Researcher says Vimentin is not specific enough. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research

[Histonet] CAP

ere is one real close. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu<mailto:b-freder..

Re: [Histonet] floor vibration

rnice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: Jeffrey Robinson via His

[Histonet] Mycoplasma

Hello all, Will PAS stain mycoplasma? Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu<mailto:b-fre

[Histonet] CAP

that long. Came up during interim self inspection. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu<mailto:

[Histonet] CD34

Been looking around a bit and seem to have it a roadblock. Does anyone know of a vendor for CD34 that will cross react with rabbit? On frozen sections, mind you. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern

[Histonet] Oil Red O

Hello all, Will Oil Red O stain phospholipids? We will be helping another lab with some work and the project out line states that Phopslipids are externalized on the cell surface during apoptosis. Can always try it I suppose. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology

[Histonet] Masson's on Frozens.

all? Tissue is rabbit liver. BErnice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu<mailto:b-freder...

Re: [Histonet] Coverslipping mystery

We have a CV5030 and use the suggested Surgipath micromount. It works well. As has been previously mentioned, you can adjust the amount of mountant dispensed as desired. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center

Re: [Histonet] Trichrome troubleshooting

dips each solution) Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: Elizabeth Chl

Re: [Histonet] : Paraffin block disposal

that person may end up on more than one trial and need a different block (like a LN when a tumor block was originally needed). Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421

[Histonet] Toluidine blue

Hello all, I was taught to do Toluidine Blue O without a control. Is there actual one and what would it be? I'm staining a bone core. Don's ask why, it's research and what a researcher wants... Plus they have a protocol they are following for this cartilaganous defect. Thanks, B

[Histonet] MTA-1 Microarrayer

Hello all, Anybody have any idea where to get the batteries for this arrayer? Regular 2450 batteries are too thick. Batteries plus was a no go. See some on Amazon but not sure if they are thin enough. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility

[Histonet] Question

All, I have a procedure here that call for and I quote "1.25 ml NaOH 10N in 1L of water." I know how to get 1 N, but how do I get 10. Having rarely hd the opportunity to make many Normal solutions ,my brain is not computing. Is it an error? Bernice Bernice Frederick HTL (ASCP) Senio

[Histonet] Schiffs

Am I missing something? I ordered Schiffs and sigma tells me it was shipped yesterday. Hello, today is Friday and last I recall Schiffs need to be refrigerated! You'd.. think they would realize this. Sorry all, have to vent. Bernice Bernice Frederick HTL (ASCP) Senior Research

[Histonet] Schiffs

Having a brain fart all- does the Schiff go into the formaldehyde or vice versa to test if the Schiffs is still good? Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421

RE: [Histonet] Kidney Stone Histology?

Do you all see the picture of the stone on the NSH facebook page? It looks like an easter egg! Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder

[Histonet] RE: cleaning glassware

We always acid clean before doing any silver stain. No metal Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu

RE: [Histonet] BS in Histotechnology

m with that answer.Grrr. Or the difference between a Mucin, Pas and Alcian Blue. The cytopath who asked did really need to know. As well I vaguely recall a question back on my HTL exam asking why a pathologist would request a mucin stain Bernice Bernice Frederick HTL (ASCP) Senior Research

RE: [Histonet] BS in Histotechnology

ave a degree in histo. I agree with all in that PI's think anyone can cut (no theory) and NU does not restrict who can but equipment or use it, despite the fact we are the core facility. We are not unionized so I envy your 36/hr. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech

RE: [Histonet] FW: Release of blocks to research facilities

Research facilities usually pay the submitting institution for submitting the block to the study. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b

RE: [Histonet] Acetone fixing and tissue damage

We use a 3:1 ration of cold acetone/100% ETOH to fix frozens for IHC. Slides are always air dried first to remove any moisture. Usually 75 mls acetone,25 mls alcohol. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University

[Histonet] Old slides.

recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N

RE: [Histonet] Controls:

There was actually an article written in The Journal of Histotechnology a few years back on this and it was, I f I recall, using a hot dog to create the gram control. Might be in the archives on nsh.org. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H

RE: [Histonet] Automatic H & E slide stainer recommendations

I agree as well. The XL is a wokhorse. We have the transfer station and the CV5030 to go with it. Bernice Frederick (HTL) ASCP Senior Research Tech Pathology Core Facility Robert H. Lurie Cancer Center Northwestern University 710 North Fairbanks Court Olson Room 8421 Chicago, IL 60611 312-503

[Histonet] TMA arrayer

Hello all, If anyone has a semi-automated arrayer, where did you get it? We have one from Veridiam, but are wondering if anyone else makes them. We are looking to purchase another unit. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer

[Histonet] RE: Specimen numbering systems

H use the system. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet

RE: [Histonet] Re: Flames at embedding centers

Darn right! We'll live forever at this rate of "fixation"... Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@n

[Histonet] RE: Rubber "mats" for pinning specimens

affin. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet

[Histonet] RE: Histology Tracking systems

as of yet, but it takes time. Once the requisition has been made we go in and make as completed for each task we perform. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chica

RE: [Histonet] On the lighter side...

Or we'll live forever..... Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message-

RE: [Histonet] Are Paraffin Blocks Biohazard

Same for IATA (shipping )We have to take the training every 3 years... Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder

RE: [Histonet] On the lighter side...

What's that in microns??? Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message-

RE: [Histonet] On the lighter side...

29. I feel so young... Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From

RE: [Histonet] Peggy Wenk

This is the announcement on the NSH page: http://nsh.org/content/peggy-wenk-true-mentor She will be missed! Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL

RE: [Histonet] Histology as art!

Love it! There is a tech that makes jewelry that sells at NSH. Made in a variety of special stains... Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312

[Histonet] RE: rolling sections

True. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun

[Histonet] CAP at NU

Oh yay! CAP is visiting us today. Why do they send an MT to do histo? And a snarky one at that.. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312

[Histonet] RE: Freezer for tissue storage

We use -80 uprights and even with a digital temp readout we have a chart on all of them (about 20). We are a tissue bank as well as doing research. We have what we call a freezer farm. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie

RE: [Histonet] HT HistoDeck question...

We fix H&E's in 95% and our IHC protocol is acetone/alcohol fixation. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723

RE: [Histonet] Shipping Slides

Same here and then in a box with more bubble wrap or we use some of those disposable bench underpads for space filler. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421

[Histonet] RE: Histology Video

In Providence? Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet

RE: [Histonet] RE: Competency for Anatomic and Clinical Pathology

We do the blinded sample part. Our manager has us look at an H&E stained slide and we identify the tissue (basic histo) and we are asked what stain is appropriate for the tissue (special stain or IHC) Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Rober

RE: [Histonet] Isopentane storage-the polling is open

Always in an explosion proof refrigerator for storage. The isopentane in use is in an isopentane tank (at -70C) in the frozen section area of the gross room as is the fridge Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center

[Histonet] FW: Primera Printer

>From my manager Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu<mailto:b-freder...@northwestern.edu&

RE: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin)

We only babysit when we receive outside slides or the label was not removed prior to staining. It can be adjusted to fit the parameters of the slides used. We have the XL and connected coverslipper. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H

RE: [Histonet] Pregnancy

We don't even let them near the stainers, processors or coverslippers. Those I've seen that are expecting can't deal with the smell anyway. It's a teratogen. I wouldn't risk it either. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility EC

[Histonet] RE: QC Slide retention

We keep ours for 2 years- kind of like those lovely QC sheets for CAP. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder

[Histonet] RE: slides and blocks discards

Correct- we had to learn all this for IATA training (how to package and properly ship biological samples). There's a massive list. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fair

[Histonet] RE: Microtome blades

VWR Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun

RE: [Histonet] 88305TC starting to hit the fan...

But can they afford to buy it Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message

RE: [Histonet] Devasting news on 88305TC component

Bear in mind it only 88305. 't's not the only CPT code we use for billing. Just all those biopsies..Yes, that will mess up those independent labs that just do biopsies. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lu

[Histonet] RE: disposal of formalin

Our department of Research Safety picks it up (5 gal) container. They are responsible for disposal. I believe it goes offsite. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court

[Histonet] RE: Eosin

We add our own 1% phloxine in 95% alcohol. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original

[Histonet] RE: counterstain for Alcian Blue (ph2.5)

As does Rowley Biochemical. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From

RE: [Histonet] automated H+E and cover slipper

I second this!!! Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun

[Histonet] Beecher

All ,I know this came up before,but who took over Beecher? We need to order some more punchers. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL

[Histonet] A poem

Themselves Histotechnologist Lifts tall buildings and walks under them Kicks locomotives off the tracks Catches speeding bullets in their teeth and eats them Freezes water with a single glance They are God - Anonymous Berni

[Histonet] RE: CAP

We got an A+ rating. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From

[Histonet] RE: Friday funny...block alignment

Haven't had that checked lately. How about you? I know my mom may have had something to say to that effect back in high school...... Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks

RE: [Histonet] BLock alignment tool

As does TechOne Biomedical. www.techoneweb.com. We have one for each microtome. Just bear in mind if your floor or workstation is not level, it may skew the leveling of the microtome. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer

[Histonet] CAP

They're here!!!!! Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu<mailto:b-freder...@northwes

RE: [Histonet] Unregistered techs

All, My point exactly. It belittles what we do (our education and training) and went through to get certified. As I voiced to a an MD the other day, it would be like him calling himself an MD without being licensed. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core

RE: [Histonet] Unregistered techs

Than you Jackie. That was the point I was trying to make. I like my job and I hate it when researchers think "anyone can do histology", yet even though the MD has to have us tell him or his Post-docs what they need or do not need. Bernce Bernice Frederick HTL (ASCP) Senior Res

RE: [Histonet] (no subject) (Not requiring HT Certification)

So why was OJT supposedly off the charts in 2005 (so to speak). Guess not. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder

RE: [Histonet] (no subject) (Not requiring HT Certification)

of these people never went to histo school. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu

RE: [Histonet] Aetna requiring CAP accreditation for non-hospital labs

new therapies). Don't mess with us techs not in a hospital. I did it for 20 years. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3

RE: [Histonet] Histobath

Fisher owns Shandon. Part of Thermo-fisher. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original

RE: [Histonet] Retention Standards

sletter.html&_state=maximized&_pageLabel=cntvwr Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Origin

RE: [Histonet] Water Quality

Our DI tanks are provided and serviced by Seimens. They do the testing. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder

RE: [Histonet] Processor Preferences??

If it was me and it is in the future, I want a Leica Peloris. Leica also has a new one that is a single chamber version of the Peloris. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N

RE: [Histonet] Diamond Knives

We have a DDK knife, works great on undecalcified bone. We send it to Dorn and Hart Microedge for resharpening as they are local to us and have been at it for years. They used to sharpen our old microtome knives. Talk about dating yourself! Bernice Bernice Frederick HTL (ASCP) Senior Research

[Histonet] annual salary survey

Has there been a new salary survey done yet? I have the one from last January's Advance magazine. Anyone seen another one lately? Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fair

RE: [Histonet] saffron vs. safran du gatinais

We buy our alcoholic saffron from Rowley biochemical. Already made and reusable. We were still using it when it hit its expiration date. You can but all the Movat's reagents as a kit or but what you need. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility EC

RE: [Histonet] Amazing

We use .5% acid alcohol ( hydrochloric) with Harris hemo. No problems. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder

RE: [Histonet] Control Slides

Same here René, unless we can absolutely not find a positive case. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder

[Histonet] RE: Fibulin 3

Or does it detect only 3??? Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From

RE: [Histonet] Staffing to work ratios

(probation) to get the quality and quantity of techs that had been around for at least 10 years and I was straight out of histo school. Well taught, I might add. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern

RE: [Histonet] Cat Scratch fever

Too true! Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun

RE: [Histonet] decal question

Ann Preece states acid decal uses aqueous solutions od either formic,nitric or trichloroacetic acid. Other methods mentioned are Ion-exchange resin,electrical ionization and chelation. The histo bible! Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL

RE: [Histonet] Alignment tool

So does TechOne biomedical Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From

[Histonet] Phosphogard/guard

Has anyone heard of or used this for protection of phosphorylation sites during IHC? My manager wants to try it. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421

RE: [Histonet] Automatic H&E stainer with coverslipper (glass)

Same here. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun

RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin

. You relearn/adapt your skills to do what is needed in your job- that is the reason I was hired- the hospital wanted a tech easy to retrain to their procedures. Bear in mind, OTJ is no longer a viable route (last I heard) Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core

RE: [Histonet] peggy wenk comments on HT/HTL practical

new? Their program is a CLS degree and she chose histo from that. Great tech by the way- histo is not much different the world over, from what I can see. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern Univer

[Histonet] RE: TFE-3

Same here. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Settembre, Dana Sent: Tuesday, August 09, 2011 11:05 AM To: 'Houston, Ronald'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: TFE-3 W

RE: [Histonet] What is a great manual mirotome

I actually use a Microm HM315 and have never had issues with it. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jsjurc...@comcast.net Sent: Tuesday, July 12, 2011 2:47 PM To: Patrick Laurie Cc: Histonet@l

RE: [Histonet] What is a great manual mirotome

I want my AO820 back! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie Sent: Tuesday, July 12, 2011 2:40 PM To: sdys...@mirnarx.com Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet

RE: [Histonet] Retirement

Pina coladas here.!!! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Friday, June 17, 2011 11:43 AM To: Cynthia Robinson; Bill O'Donnell; histonet@lists.utsouthwestern.edu Subject: R

RE: [Histonet] Formaldehyde in H2S's 12th RoC

Well we still use Hematoxylin powder, Xylene ,Oil Red O, Beibrich scarlet and Congo red and we haven't been stopped yet. I'd think embalmer would be well-fixed and preserved ,rather than have a high mortality rate. Have the proven it's from formaldehyde? Whom knows what the get from a dead body? B

RE: [Histonet] question on H pylori

We did diif -quik or Alcian Yellow. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Harrison, Sandra C. Sent: Friday, June 10, 2011 9:10 AM To: Setlak, Lisa; histonet@lists.utsouthwestern.edu; histonet-requ

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