Happy Wednesday Everyone
Has anyone been having consistency problems with their p16 staining since
MTM has been taken over by Ventana?
One day the procedure works another day it doesn't. Same procedure, no
variation in the temperature on pretreatment, run on the Dako 48, with no
errors.
-celllab.com
-Original Message-
From: Ross Benik [mailto:r...@premierlab.com]
Sent: Wednesday, February 06, 2013 1:26 PM
To: Cynthia Pyse
Subject: RE: [Histonet] p16
Do you monitor the temperature and humidity inside the Link48? How often do
you prepare your retrieval solution/are you using
Hi Histonetters
We are currently having a problem with our Medicare reimbursement on the
tech component of the 88305. If the patient has been seen at a hospital,
either in-patient or out- patient, we are told by Medicare that we have to
contract with that hospital to bill for the 88305. The
)
Laboratory Manager
X-Cell Laboratories
20 Northpointe Parkway Suite 100
Amherst, NY 14228
716-250-9235 etx. 232
e-mail cp...@x-celllab.com
-Original Message-
From: Brendal Finlay [mailto:brendal.fin...@medicalcenterclinic.com]
Sent: Tuesday, January 29, 2013 2:32 PM
To: Cynthia Pyse
Cc: histonet
Good afternoon histonetters
We are currently investigating bringing Her 2 Neu CISH staining into our
lab. FISH staining has too many logistics nightmares for me to consider, not
to mention the cost of purchasing digital scanning equipment. Our
pathologist are off site in 5 different hospitals.
Happy Monday Histonetter
Who out there in Histoland is doing the testing for BRAF, KRAS, ALK, and
HER2neu and what testing methods are you using. I prefer not to use FISH.
Would rather have a permanent record of the slides. Any information is
welcome. Have a great week.
Cindy
Cindy Pyse,
Yes you are.
Cindy
Cindy Pyse, CLT, HT (ASCP)
Laboratory Manager
X-Cell Laboratories
20 Northpointe Parkway Suite 100
Amherst, NY 14228
716-250-9235 etx. 232
e-mail cp...@x-celllab.com
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
I want to thank everyone who responded to my chemical disposal question. I
now have adequate ammunition to combat my general manager's lack of
knowledge. He seems to think I don't know what I am talking about. Thanks
again for the backup it is appreciated. Have a great weekend.
Cindy
Cindy
I would also be interested in the answers to this.
Cindy
Cindy Pyse, CLT, HT (ASCP)
Laboratory Manager
X-Cell Laboratories
20 Northpointe Parkway Suite 100
Amherst, NY 14228
716-250-9235 etx. 232
e-mail cp...@x-celllab.com
-Original Message-
From:
Quick question for Histoland. I am having a debate about DAB disposal. Our
general manager ( non lab background) insists that the liquid DAB can go
into a biological hazardous waste. I disagree, it is a chemical and needs to
be disposed in the chemical hazardous waste. What is everyone else doing
Rhonda
I have both a Thermo-Fisher Excelsior and a Sakura VIP6. Both work well but
I prefer the Excelsior. There is less hands on time for changing reagents,
flushing and cleaning. I did have problems at first with the VIP. Since
Sakura replaced the gate valve all has been great. If I need to
Happy Wednesday Histonetters
I have had a request from some of our clients to do epidermal nerve fiber
density testing. I have found information in how to obtain the specimen but
have not found the protocol for processing, cutting, and staining the
specimen. To anyone out there who is
We also use safranin on all our small biopsies and also our breast biopsies
with no adverse effects to any of our IHC or ISH.
Cindy
Cindy Pyse, CLT, HT (ASCP)
Laboratory Manager
X-Cell Laboratories
716-250-9235 etx. 232
e-mail cp...@x-celllab.com
-Original Message-
From:
Andrew
You could use Clearium from Leica. Clearium can either be coverslipped from
xylene or isopropyl alcohol. Drying time from isopropyl is longer then
xylene.
Cindy
Cindy Pyse, CLT, HT (ASCP)
Laboratory Manager
X-Cell Laboratories
716-250-9235 etx. 232
e-mail cp...@x-celllab.com
I know. I was hoping to see what everyone else is doing.
-Original Message-
From: Kim Donadio [mailto:one_angel_sec...@yahoo.com]
Sent: Friday, May 04, 2012 6:30 PM
To: Cynthia Pyse
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] vaginitis panel
It's Friday :)
Noticed
Histonetters
For those of you performing vaginitis panels, how are you billing these? Do
you bill for each separate test or use the combine CPT code? We are having a
discussion on what is our best method of billing. Thanks in advance for the
info.
Cindy
Cindy Pyse, CLT, HT (ASCP)
Laboratory
Karen
I would just purchase a timer to plug the Decloaking chamber into, I use one
to heat my water bath for pretreatments prior to by techs coming in. I
purchased the timers from Fisher, cat. #666224 for $28.82. Works like a
charm.
Cindy
Cindy Pyse, CLT, HT (ASCP)
Laboratory Manager
X-Cell
What do you do if the IHC slides need to be sent to another facility.
Currently we place a control on every slide we run. I know this is using a
lot of tissue but I sometimes have 3 different cases going to 3 different
facilities for patient treatment. Also we stain all the IHC stains for 5
We had one of our clients looking into this testing. The company does a hard
sell. What they neglect to tell you is the insurance companies may not cover
this testing leaving the patient with a huge bill sometimes $700 to $800.
Known Error will tell you it is a Medicare approved test what they
If the pod lab is in NJ and the reading lab is in NY, which guide lines do
you follow. NYS requires us to save our blocks for 20 years. Due to storage
issues, I would rather the pod lab store the blocks.
Cindy
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Joanne
What Dako platform are you using, link or 48? On the link I pretreat with
PK, the IS series antibody is used with an incubation time of 10 minutes..
On the 48 I pretreat with High pH TRS, the IR antibody is used with an
incubation time of 20 minutes. Both systems give me clean staining.(I'm
Good Morning Histonetters
I am looking to add EGFR to our antibody list. What clone is everyone using
out there in Histoland? Any info would be greatly appreciated. Thanks in
advance.
Cindy
Cindy Pyse, CLT, HT (ASCP)
Laboratory Manager
X-Cell Laboratories
716-250-9235 Ext. 232
e-mail
NY in winter has low humidity, I still have no problems with my Dako
stainer. Make sure the lid is closed. I had a tech who consistently left the
lid open, there was some drying on the first row of slides. Closed the lid
the problem stopped. Are you using Dako buffer? The surfactant in the buffer
Good Morning Histonetters
One of our clients is interested in starting to compare biopsy tissue with a
DNA swab. The test name is Known Error test and it is performed at a company
in Utah. Does anyone out there know what this testing entails. I could use
any information since I have no knowledge
Hello Histonetters
I need to bleach melanin out of some paraffin sections. The only bleaching
solution I have is a 3% H2O2. I know you can bleach melanin in a 10% H2O2
solution for 1 to 2 days. How long do you think it will take to bleach the
section in a 3% solution? Of course everyone wants it
Hello Histonetters
Happy Tuesday. I hope everyone had a Merry Christmas. It is amazing how
times flies when you have time off. Now it's back to the old laboratory
grind.
We are in the market for a new immuno stainer. I currently use a Dako Link,
which I am very pleased with. I have demoed the
Hi Histonetters
I am looking to add to our test menu the High and low risk HPV. What is
everyone using out there. Instrumentation? Vendor? Pros and cons to any
system. I would appreciate any input. Thanks in advance.
Cindy
Cindy Pyse, CLT, HT (ASCP)
Laboratory Manger
X-Cell Laboratories
Martin
We currently have a Leica CV35030, which is a glass cover slipper. My 15
Pathologists prefer the glass covers slips for the optical quality. We are
required to archive our slides for 20 years, which is another reason we
chose glass cover slips.
The Leica CV5030 if cleaned nightly and
I would be interested in everyone responses.
Cindy
Cindy Pyse, CLT, HT (ASCP)
Laboratory Manger
X-Cell Laboratories
e-mail cp...@x-celllab.com
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bull,
Hello Histonetters,
Recently we have seen an increase number of unstained slides requested from
outside facilities. We do not release our blocks, so unstained slides are
the only option for any facility to obtain our tissue. Currently we do not
charge for these slides, but I am rethinking that
Market Lab cat#ML1212. I would check the cat# before ordering, the picture
in the catalog show the right rack but the description is different.
Cindy Pyse, CLT, HT (ASCP)
Laboratory/Histology Supervisor
X-Cell Laboratories
e-mail cp...@x-celllab.com
-Original Message-
From:
Thanks to everyone for the Cam5.2 info. A great weekend to all.
Cindy
Cindy Pyse, CLT, HT (ASCP)
Laobratory/Histology Supervisor
X-Cell Laboratories
e-mail cp...@x-celllab.com
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
Hello Histonetters
What company is everyone buying their Cam 5.2 antibody from? Thanks for the
information.
Cindy Pyse, CLT, HT (ASCP)
Laboratory/Histology Supervisor
X-Cell Laboratories
e-mail cp...@x-celllab.com
___
Histonet mailing list
Most clinician I know have other staff in the procedure room. Why not have
someone other than the clinician place the specimen in the formalin. This
way you get the best possible fixation for the patient.
Cindy
Cindy Pyse, CLT, HT (ASCP)
Laboratory/Histology Supervisor
X-Cell Laboratories
e-mail
Hi Histonetters
I have 2 tech off on vacation so I thought I would take the easy way this
time. Does anyone know the difference between distilled water system and the
reverse osmosis water system? Could if effect the IHC staining by using one
or the other? Usually I would research it myself but
As long as we don't have to go back to steel knives, old AO micrtomes and
the autotechnicon I'm in. I am also 15 to 18 years out. What is the world of
histology going to do without us. Who will know how to make a solution of
mucicarmine (not that I do anymore, but I could) or eosin? The tech I
Happy Friday Histonetters
Does anyone have a validation log sheet they have used for the validation of
a recycler. Someone spilled xylene on our copy then proceeded to discard it
without coping the sheet first. I would appreciate it if anyone would share
the log sheet they have used, it would
Hello Histonetters
What is everyone using to decal specimens. I haven't had to use it in a few
years, before that I just purchased formic acid and made a 10% solution.
Just wanted to see if the criteria has changes over the years. Thanks in
advance for the responses.
Cindy Pyse, CLT, HT
Hi Histonetters
I am testing the Leica Bond III immunostainer. How is everyone handling
processing the Hercept test from Dako on the Bond III? I cannot use either
the approved pretreatment or visualization system, not to mention the
approved buffer. I currently run my immunos on the Dako so it
Good Morning Histonetters
For those of you who have pap smears in their facility's, who do you have
preparing the pap smears. Lab assistants, lab tech, or lab technologists? I
am having a discussion with my general manager on who can prep pap smears. I
decided to take a quick poll to see what
Do you use a hydrophobic pen to circle the tissue. This concentrates the
solution where you need it.
Cindy Pyse, CLT, HT (ASCP)
Laboratory/Histology Supervisor
X-Cell Laboratories
716-250-9235 etx.232
e-mail cp...@x-celllab.com
-Original Message-
From:
Sabeti
I use the AEC chromogen (Dako catalog #K346989) for my melanoma cases. I use
a aqueous mounting media, placed on the tissue only, dry it completely on a
hot plate. When dry I coverslip it with permanent mounting media(don't
place it in xylene), dry coverslip. Works great, and the color
Cindy
I use a Fisher Isotemp 205 waterbath. The end result is beautiful.
Cindy
Cindy Pyse, CLT, HT (ASCP)
Laboratory/Histology Supervisor
X-Cell Laboratories
716-250-9235
e-mail cp...@x-celllab.com
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Practice, practice, practice!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jenny Vega
Sent: Monday, February 28, 2011 5:19 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] How many tissues an
Carol
We mark all our prostates with dye for ease when embedding. For prostates
that need to be accessioned in order we alternate the color of the dyes,
hematoxylin, safranin, eosin. When grossing, the color of the dye used is in
the dictation. Hope this helps.
Cindy
Cindy Pyse, CLT, HT (ASCP)
Happy Friday Histonetters
I want to thank everyone for the information on IHC strainers. This is just
what I needed. People who actually use the machines on a daily basis giving
their opinions. What a great forum we have. Hope everyone has a great
weekend. Currently we are receiving 6-10 inches
Hi Histonetters
I currently use a Dako stainer for my IHC staining. It is a work horse with
very little problems. It is a older model that we may need to replace in the
near future. What is everyone using out in histoland. I would be perfectly
willing to purchase another Dako but I want to
Hello Histonetters
We have recently added the BD Viper and Affrim testing to our lab. Training
is underway. I would like input from the experts out there on how much time
is actually spent on setting up both the Viper and Affrim testing. I know
what the manufacturer says I just want the opinion
Happy Monday Histonetters
I am looking for a reasonably priced, non-latex glove that will hold up to
xylene. The purple knight from Kimberly-Clark works great but is cost
prohibited. My general manager want me to find a cheaper alternative. I have
had several samples but none will hold up to any
From: Cynthia Pyse [mailto:cp...@x-celllab.com]
Sent: Tuesday, September 28, 2010 3:48 PM
To: 'histonet-requ...@lists.utsouthwestern.edu'
Subject: DAB
Hello Histonetters
I need some expert advice concerning the disposal of DAB. Our lab is in NYS
and there has been some discussion
Help!
I am having a problem with me VIP6 tissue processor. About every 4 to 6
weeks the processor has a error code that position 2 which is 80% has not
pumped in fully in the allotted time. Sakura answers to this problem is
either I need to use their formalin or the alcohol in the second station
Thanks everyone for your responses. Sakura has scheduled an upgrade of my
rotary and gate valve. I hope this is the answer I need.
Cindy
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
I second Linda's technique, the only other option I use is to bring the
slide up at a negative angle so the slide comes out of the water bath first
then the section. I find this works well for us.
Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cp...@x-celllab.com
Cathy
Have you tried adding an EnVision FLEX mouse linker to your protocol.
Sometimes that will work.
Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cp...@x-celllab.com
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
I use tattoo dyes. They work great and are much cheaper than tissue marking
dyes.
Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cp...@x-celllab.com
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
We have a contract with the hospitals, this comes from our billing manger.
We bill the hospitals, then they bill the patient.
Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cp...@x-celllab.com
-Original Message-
From:
I am also looking for the same type of waterbaths and would appreciate any
information.
Thanks
Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cp...@x-celllab.com
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
I'm relieved that someone else is having the same problem. I was beginning
to think it was my processor. I have already decided to switch paraffin.
Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cp...@x-celllab.com
-Original Message-
From:
Christi
We run a diff stain on all gastric bx suspected of H. pylori as routine, it
saves us time.
Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cp...@x-celllab.com
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Happy Friday Everyone
What clone is everyone using for the CD68 antibody for FFPE human tissue?
Thanks for the info in advance. Everyone have a great weekend.
Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cp...@x-celllab.com
Thanks to all for providing me with the information I requested. You have
made my decision making so much easier.
Thanks again
Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cp...@x-celllab.com
___
Histonet
Happy Friday Histonetters
What is everyone using for controls for FNA IHC stains. We routinely stain
human FFPE sections and only have controls for tissue. Recently we had a few
cases when the only sample was an FNA to perform IHC on. Any suggestions
would be welcome .
Regards
Cindy Pyse,
, April 09, 2010 12:40 PM
To: Cynthia Pyse; Histonet
Subject: RE: [Histonet] IHC on FNA
This topic touches a nerve with me. We recently went back to receiving
requests on FNAs after years of not doing any. When we were routinely
staining them with IHC, we would use a frozen tissue section
That is how I started many moons ago(29 years and counting). I spent 1 week
in histology, the next week in cytology, this continued for 2 years. I
currently supervise both the histology and the cytology lab sections. My
histotechs do little cytology prep work but are willing if time allows to
help
Leica CV3050
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margaryan,
Naira
Sent: Thursday, March 25, 2010 12:07 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] coverslipper
Hi Colleges,
I
Hi Histonetters
I am having a senior moment. Can anyone tell me what McCoy fixative is made
of and what it is used for? Thanks in advance for the help.
Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cp...@x-celllab.com
I just place the slides into 80% ETOH for 2 minutes.
Cindy
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
cscam...@uci.edu
Sent: Monday, March 15, 2010 7:42 PM
To: HistoNet
Subject: [Histonet] Removing
Matt
We have both the VIP 6(new)and the Excelsior(older). The Excelsior is a
little less user friendly but is a workhorse if maintained properly. There
is less solution exposure since you do not have to fill the containers as
you do in the VIP6. Both machines produce excellent tissue blocks. The
We purchase our p16 through MTM laboratories reference # 9517.
Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cp...@x-celllab.com
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf
We purchase it from Biocare. Protocol: pretreatment Diva (Biocare) 20
minutes water bath 20 minutes cool down. Detection system Mach 4 (Biocare)
10 minutes H2O2, 10 minute primary antibody dilution 1:100, 5 minutes mouse
probe, 12 minutes polymer, 10 minutes DAB(Dako), 5 minutes
hematoxylin(Dako).
Fontana-Masson also stains for Argentaffin granuals.
Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cp...@x-celllab.com
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Cathy
I prefer the glass coverslips. It seems to give more protection to the
tissue. Recoverslipping is also easier than with the tape. Just my opinion.
Hope this helps.
Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cp...@x-celllab.com
-Original Message-
Anne
You might want to try going up the Sakura chain of command. I had trouble
with a coverslipper, after contacting the regional manager it was amazing
the service it received. Good luck.
Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cp...@x-celllab.com
Tom,
I agree with you about Nate qualifications. Unfortunately in NYS if you
bring in outside work your tech MUST be licensed in NY. The copy of the
license need to be displayed in the lab. I haven't found any way to hire
employees without a license.
Cindy Pyse, CLT, HT (ASCP)
Histology
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