RE: [Histonet] Block Counts

I have always found there are so many variables that having an expectation of setting a set number is not always possible. I feel they should be compared to their own standards and not of their co-workers. If they can cut or embed a set number on a particular than they should maintain or

RE: [Histonet] Aspergillus tissue blocks for controls

As well we have used mushroom and other moldy food products but it only demonstrates the mold and not tissue elements. But you can mince tissue and mix it with the fungus or mold so it one big segment and this gave more desirable results. Diana -Original Message- From:

[Histonet] Endometrial biopsies

In the past when we receive these samples there has been no issues cutting them. Over the past few months there has been a tendency for these blocks to shred into fine strips when cut. Almost like there was sand in the blocks. Mollifex, decal, warm or cold, doesn't matter they still shred.

[Histonet] PowerPath QA

Is anyone using this module and are you willing to share any documents regarding it. Or opinions on what think of it for a QA program Sincere thanks Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] cryomolds without a cryobar

There was a recent post for someone inquiring about cryomolds. I have not used these and have researched them and found out the Sakura cryostat has a cryobar with recessed ports to accommodate these molds. Has anyone used these on a Microm microtome. Is there a problem removing the molds and

RE: [Histonet] Looking for cryomolds

I did not know such a product existed until now. How do these molds work? Diana McCaig Histology Lab Chatham Kent Health Alliance 80 Grand Avenue West Chatham. Ontario N7L 1B7 519-352-6401 (6604) This email communication and any files transmitted with it may contain confidential

[Histonet] Bone marrow blocks

I sent out a request for information a while back and am going to ask again from a different approach. I am finding problems with embedding blocks of bone marrow particles with or without blood clot. Does anyone have a special technique they would like to share to concentrate the particles

[Histonet] bone marrow aspirations

Can anyone share with me their process for processing bone marrow aspirations and embedding them in paraffin blocks. Currently ours are being spread out throughout the entire base mold and makes it cumbersome to screen. Has anyone used histogel or making a button and embed that instead of

[Histonet] Reagent containers for VIP5

Does anyone have spare reagent containers for a VIP5 they are willing to sell. I am looking for 3 to use exclusively for warm water wash. We are located in Ontario, Canada Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] standards for embedding

I know this topic has been beaten to death in the past and do realize it is dependent on tissue orientation, quantity of pieces in a block, and experience. There are guidelines suggested by the College of Med Lab of Ontario in a document Oct 2008 that indicates the expected minimum daily range

[Histonet] Alcian Blue

What is the shelf life of prepared 1% Alcian blue in 3% Acetic Acid? Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Cresyl violet stain for HP

Is anyone willing to share their staining protocol for Cresyl Violet stain for HP Thanks Diana McCaig ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] LEICA 6025 PROCESSOR

We are going to be setting up a Leica 6025 Processor. Can anyone send me their SOP and worksheets that you have created so I can use them as a reference and modify them according to our usage. Would truly be appreciated as well as any helpful tips or tricks you have encountered. Diana

[Histonet] decal affecting IHC

Does decalcifying tissue (Calex II) affect the antibody reaction for IHC in bony tissue? Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Stain for HP

What stain are you using for HP?--Giemsa. Warthin Starry or IHC. Do you do them routinely or only when requested? Are they done on an autostainer or with a kit? Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Breast processing times

Can you please give me your routine for processing breast cases over the weekend. Do you extend the time in alcohol to achieve the 48 hour maximum, call someone in over the weekend, or just indicate on the patient report that the fixation extended beyond the 48 hours limit for HER 2, 72 hours for

[Histonet] thickness of slides

Can you tell me what thickness you cut your routine slides for HE and immuno (in particular lymph nodes). Also, your protocol for cutting prostate needle core biopsies.how many spares you cut and do you designate the level on the slide label if multiple levels are submitted on one slide?

[Histonet] air drying special stain slides rather than dehydrate and clear

I was hoping to get information on why special stains are dehydrated, cleared and mounted vs allowing them to be blotted dry, air dried then coverslip. Every procedure I have ever encountered always indicates to dehydrate and clear but I have heard where some labs are blotting the slides ,

RE: [Histonet] RE: air drying special stain slides rather than

Would this work for auto cover slipping (tape film)if they were set in the xylene reservoir prior to cover slipping? Diana -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of E. Wayne Johnson Sent:

[Histonet] counterstain for Alcian Blue (ph2.5)

Can you please let me know what options for counterstain there are other than Nuclear Fast Red or a supplier who sells the prepared solution Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] restaining negative stain with synaptophysin

We have no tissue in a block and would like to take the negative control and stain it for synaptophysin. We are using the Nemesis, Biocare Mach 4, AP protocol. Sincere thanks Diana McCaig ___ Histonet mailing list Histonet

[Histonet] Quality assurance program for pathologists

Is anyone willing to share with me their quality assurance/management program for pathologists. Sincere thanks Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] PowerPath Turn Around Time Report

Hi Does anyone using PowerPath have a turn around time report? Diana McCaig Histology Lab Chatham Kent Health Alliance 80 Grand Avenue West Chatham. Ontario N7L 1B7 519-352-6401 (6604) This email communication and any files transmitted with it may contain confidential

[Histonet] DRYING TIMES for immuno stains

Can you tell me how long you let your IHC slides air dry at room temperature and how long you put them in the oven prior to staining? Thanks Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Biocare decloaker

We are trying to validate a Biocare decloaker and have found when we use 122 degrees for 30 seconds we get great signal, but distorted morphology. If we reduce to 90 degrees for 45 minutes, the signal is significantly decreased but the morphology is good. What protocols are you using?

[Histonet] SMM-HC

We are using Biocare Smooth Muscle Myosin-Heavy Chains (AP Protocol using Warp Red and previously Vulcan Fast Red)on the Nemesis auto stainer.. Lately we have notice considerable background staining. Any suggestions. The slides almost look like in the old days when you put too much albumin ( as

[Histonet] uric acid crystals in tissue for gout

Does anyone have a method for identifying uric acid crystals in gout on a tissue sample. Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] MTS/Verhoeff's Stain

Does anyone have a procedure for a combination MTS/VG elastic stain that they would be willing to share? Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] diastase

I am looking to purchase diastase to use for the PAS/DIASTASE stain. I see there are many options in the catalogues. What is recommended? diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] negative controls on immunos

If you do a run of several immunos today and you run a negative control, and there is a request for additional immunos tomorrow, would you run another negative control with the additional slides. They are being stained on a stainer and not manually, diana

[Histonet] Control sections for frozen sections

Does anyone run a HE control prior to staining a frozen section? Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] PAS STAIN

Hi We have been doing a PAS stain on the same control block and same reagent supplier for a long time. Yesterday when we ran the slides, they failed to stain We opened new bottles of Periodic Acid and Schiff's Reagent and recut fresh controls Still, we are unable to get any staining.

[Histonet] inking dyes

Can someone provide me with a supplier of tissue marking dyes for use on the grossing bench. Diana McCaig Histology Lab Chatham Kent Health Alliance 80 Grand Avenue West Chatham. Ontario N7L 1B7 519-352-6401 (6604) This email communication and any files transmitted with it may contain

[Histonet] AB-PAS for Barrett's Esophagus

Can anyone share with me their procedure for AB-PAS for Barrett's Esophagus. With thanks Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] processing schedule for needle core biopsies

We have been notices folds and wrinkles in the needle cores biopsies that do not seem to be affected by increased soaking. I am inclined to think it has to with processing. Currently they are done with the routine surgical specimens .What is the preferred schedule for needle cores? Is it best to

RE: [Histonet] HPS Stain

I think there is confusion regarding the original question posed. They were not asking about the HPS stain. Some hospitals use an eosin/saffron stain in their routine HE. I had another lab recently asking about the formula to prepare this solution instead of eosin. Any advise? Diana

[Histonet] minimum fixation time for needle core prostate biopsies

Is there a minimum time for prostate biopsies to be fixed. Does fixation times affect PIN4's? Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] cassette labelers with bar codes

Can anyone advise me on cassette labelers that print 2D bar codes? Preferences or suggestions what to look for. Diana McCaig Histology Lab Chatham Kent Health Alliance 80 Grand Avenue West Chatham. Ontario N7L 1B7 519-352-6401 (6604) This email communication and any files transmitted

[Histonet] Alcian Blue

In the past, when we made up 1% Alcian blue in 3% acetic acid (using bottle distilled water), the pH was always 2.5. We did not have to modify it to get the proper pH. Still using the same powder, we are getting a pH of 2.1. I hate to adjust it because it seems to affect the staining. Any

[Histonet] IMMUNO STAINING--DECOLORIZING

We had a series of PIN 4 cocktail immuno stains and got a poor reaction. I am afraid to cut deeper in the block and miss the area of concern. I have determined the problem was due to the heat bar not being turned on. What can I do the slides to reverse the process and restain them. The DAB

[Histonet] ER/PR scoring guidelines

Can someone provide me with the guidelines (Canadian) for scoring ER/PR and what the preferred antibody is for determining this. I see there are several clones. Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] (no subject)

What should the pH range be for Scott's Tap Water? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet