Re: [Histonet] LDT's Regulatiions by the FDA

Histonet does not allow attachments. -Original Message- From: Histology via Histonet Sent: Wednesday, June 26, 2024 8:13 AM To: Paula ; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] LDT's Regulatiions by the FDA EXTERNAL SENDER - Exercise caution with requests, links, and

Re: [Histonet] What is the best xylene substitute for histology?

We have used both XS-3 and Clear-Rite 3 with success in processing and staining. They are not as efficient as xylene, so you will need to extend the times a bit to account for that. We have had the most success with Permount. No fading or incompatibility. Jennifer -Original Message-

Re: [Histonet] 100% ethanol in tissue processing

Absolutely can be used on the tissue processor or for staining. -Original Message- From: Curt Tague via Histonet Sent: Wednesday, May 29, 2024 12:16 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] 100% ethanol in tissue processing EXTERNAL SENDER - Exercise caution with

[Histonet] California Society for Histotechnology Symposium

Our California Society for Histotechnology Symposium is coming up. This is a great way to earn those CEs and network with fellow histotechs. Please share this information with your colleagues. You will find more information on our

[Histonet] RFID Tracking

Good afternoon, Does anyone use RFID to track specimens in their laboratories? Thank you, Jennifer Wooten, BA, BS, HTL (ASCP)CM . STATEMENT OF CONFIDENTIALITY:This message

Re: [Histonet] tissue cassettes

We embed from cold and this happens if the tissue is not brought up to the temperature and the wax is melted. -Original Message- From: Carl Hobbs via Histonet Sent: Friday, February 9, 2024 11:12 AM To: histonet Subject: Re: [Histonet] tissue cassettes EXTERNAL SENDER - Exercise

[Histonet] California Society for Histotechnology Symposium

The CSH is still looking for speakers for their annual education symposium. May 2-5 in Costa Mesa, California. Please contact me if you are interested in presenting a workshop. Thank you, Jennifer MacDonald https://www.californiahistology.org/home

[Histonet] Positions for KP Chino Hills Histology

We have three positions that have gone external for KP Chino Hills Histology. Our On-Call positions (per diem) will support staffing LOAs, vacations, and special projects. The hours and schedules may vary depending on the staffing and operational needs. We anticipate growth this year and look

Re: [Histonet] Microcredentials for Histology

With route 2 the applicants must have at least one year of full-time acceptable experience working histology with experience in microtomy, embedding, fixation, staining, and lab operations. They will be required to provide proof of the employment/experience to the ASCP with their transcripts.

Re: [Histonet] Moderate vs High Complexity for routine histology

Good morning, Under CLIA, Histology processes (processing, embedding, cutting, staining) are considered pre-analytic (not testing) and are not classified under either complexity level. Currently, the only activities that are considered high complexity are grossing, ISH, and reporting results.

Re: [Histonet] Stored frozen fatty muscle tissue

Are you allowing them to come up to the temperature of the cryostat before cutting? -Original Message- From: Gerard Spoelstra via Histonet Sent: Tuesday, September 26, 2023 3:45 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Stored frozen fatty muscle tissue EXTERNAL

Re: [Histonet] Von Kossa staining

I have used a UV light like the ones for indoor plants and one that is in the fume hood for disinfection. Both proved successful. Jennifer -Original Message- From: Charles Riley via Histonet Sent: Wednesday, July 26, 2023 11:23 AM To: histonet@lists.utsouthwestern.edu Subject:

Re: [Histonet] (no subject)

We have good success with the Twort. -Original Message- From: Rhonda McCormick via Histonet Sent: Monday, July 3, 2023 11:22 AM To: Histonet Subject: [Histonet] (no subject) EXTERNAL SENDER - Exercise caution with requests, links, and attachments. Hello, I'm wondering if anyone does

[Histonet] Fountain Valley Histology technician

Bio-Path has an open position for a histotechnician. If interested or if you have any questions about the position, please contact Paula Lucas at plu...@biopath.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] UCSD clinical lab is hiring Histotechnologist II and a Hospital Lab Technician

My lab is currently looking to hire 1 Histotech and 1 Hospital Lab Tech. New graduates are very welcome to apply. Willing to train. If you have any questions or would like to know more, please contact Sabrina at: s...@health.ucsd.edu. Thank you! Regards, Sabrina

Re: [Histonet] Histonet responses

Hi Anthony, If you hit reply only you will get a response. If you hit reply all a response will go you and the Histonet. Jennifer -Original Message- From: Anthony Lapiana via Histonet Sent: Tuesday, June 13, 2023 11:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet]

Re: [Histonet] IHC gunk

Is it possible that it is hematoxylin precipitate? -Original Message- From: Karen Heckford CA-San Francisco via Histonet Sent: Wednesday, March 22, 2023 10:45 AM To: Histonet Subject: [Histonet] IHC gunk EXTERNAL SENDER - Exercise caution with requests, links, and attachments.

Re: [Histonet] Specimens for Gout

They should be fixed in absolute alcohol and can go on the processor at the absolute alcohol step, or they can go into the xylene if completely fixed and dehydrated. -Original Message- From: Piche, Jessica via Histonet Sent: Monday, March 20, 2023 8:24 AM To: Histonet Subject:

Re: [Histonet] What is considered Grossing according to CLIA and CAP? Definition?

Per the grossing definition in ANP.11610 below, grossing is defined as a tissue examination requiring judgment and knowledge of anatomy. This includes the dissection of the specimen, selection of tissue, and any level of examination/description of the tissue including color, weight, measurement

Re: [Histonet] Cassette printers

We had a GD DirectMark that was marketed to print on 3 sides, but it would jam so much just printing on 1 side that we never could explore printing on the 3 sides. Jennifer Wooten, BA, BS, HTL (ASCP)CM -Original Message- From: Paula Sicurello Sent: Wednesday, December 28, 2022 11:44

Re: [Histonet] Advice for new grads?

The world of histology is very small. Do not burn any bridges. -Original Message- From: Pam Barker RELIA Solutions via Histonet Sent: Monday, May 9, 2022 9:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Advice for new grads? EXTERNAL SENDER - Exercise caution

[Histonet] Mohs Tech Position in Oakland CA

Job Description Responsibilities: * Receives and labels incoming specimens * Properly grosses tissue, inks, embeds and prepares frozen sections * Performs initial assessment under the microscope to ensure that complete epidermal and deep margins are present * Reports deviations

[Histonet] California Society for Histotechnology Annual Symposium

https://www.californiahistology.org/events/annual-symposium We are back in person for our annual meeting!! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Detection Systems

Thank you. Get Outlook for iOS From: Amos Brooks via Histonet Sent: Saturday, February 5, 2022 10:22:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Detection Systems EXTERNAL SENDER- Exercise caution with requests,

[Histonet] Detection Systems

Are labs using LSAB detection? I know the polymers are more popular, but was wondering if avidin/biotin is still being used? Thanks, Jennifer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Milestone Embedding Center

Does anyone use the HistoDream embeddding center from Milestone? Pros? Cons? Milestone rep for southern California? Thanks, Jennifer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] CAP Question

Good morning, and Happy Friday Histonet! Do any of you have a policy or response for CYP.07666, CAP's new unsatisfactory non-gyn cytology that you would be willing to share? We do few cytology cases here, this just isn't much of an issue; we've never really had to think about it. Please

Re: [Histonet] Stain line order

That step is known as differentiation. Clearing involves an organic solvent to prepare the slides for coverslipping in a synthetic mounting medium. A regressive H uses differentiation, usually with 1% acid alcohol. A progressive stain does not. Some companies use a modified progressive

[Histonet] Cryostat vacuums

Does anyone use a vacuum to clean out their cryostats, either on or off-board? Jennifer Wooten, BA, BS, HTL (ASCP)CM Technical Supervisor | Anatomic Pathology | University Hospital IMPORTANT: This message originates from TriCore Reference Laboratories or one of its affiliate organizations or

Re: [Histonet] release of body parts

We release specimens to patients here, especially because of our unique cultural demographic. If the specimen is in formalin, we will drain, rinse, and place contents in a new clean container with labels. The patient also signs a release form acknowledging that the specimen was preserved in

Re: [Histonet] Histonet Digest, Vol 213, Issue 6

We just recently purchased the Epredia NX50, which we use with our older HM525s. We love it, I just wish they would make height adjustability a standard option on them! Jennifer Wooten, BA, BS, HTL (ASCP)CM Pronouns: she/her/hers Technical Supervisor | Anatomic Pathology | University Hospital

Re: [Histonet] Histonet Digest, Vol 212, Issue 1

We surface decal with decal I for about 20-30 minutes (as long as you don't need to do any molecular studies). You can usually get 3-4 good sections before its crunchy again. Jennifer Wooten, BA, BS, HTL (ASCP)CM Pronouns: she/her/hers Technical Supervisor | Anatomic Pathology | University

Re: [Histonet] Specimen tracking scenario, accountability

Hi Curt, That sounds like a very difficult position to be in, especially with many of them not barcoded. Photo documentation might be your best bet; we use the Vantage tracking system throughout our process, and the Arcos system for tracking filed blocks once completed. You might want to reach

[Histonet] HSV control

Hi all, We ran out of good patient tissue controls for HSV IHC. We recently purchased slides from Statlab (not great), Mercedes medical (even less great) and Cellmarque (best ones). But they are pricey. Does anyone have extras, or want to set up a trade? Thank you, Jen Jennifer B Titus, PA

Re: [Histonet] Prussian Blue Reaction

spital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Origina

Re: [Histonet] Prussian Blue Reaction

to be insoluble. Try potassium permanganate followed by oxalaic acid. But book says insoluble. On Monday, June 7, 2021, Mac Donald, Jennifer via Histonet mailto:histonet@lists.utsouthwestern.edu>> wrote: The instrument malfunction and it was overstained. From: John Kiernan mailto:jkier...@

Re: [Histonet] Prussian Blue Reaction

= = = From: Mac Donald, Jennifer via Histonet mailto:histonet@lists.utsouthwestern.edu>> Sent: June 7, 2021 12:47 AM To: Gudrun Lang mailto:gu.l...@gmx.at>> Cc: histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu> mailto:histonet@lists.utsouthwestern.edu>>

Re: [Histonet] Prussian Blue Reaction

to simple make a "double stain". Regards Gudrun -Ursprüngliche Nachricht- Von: Mac Donald, Jennifer via Histonet [mailto:histonet@lists.utsouthwestern.edu] Gesendet: Sonntag, 6. Juni 2021 06:34 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Prussian Blue Reaction D

[Histonet] Prussian Blue Reaction

Does anyone know of a way to remove/reduce the Prussian blue reaction? Thanks, Jennifer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Animal Histology

, CFA Culling, RAB Drury, M Gabe, P Gray, RW Horobin, G Humason, JFA McManus, AGE Pearse. John A. Kiernan Dept of Anatomy & Cell Biology University of Western Ontario London, Canada = = = ________ From: Mac Donald, Jennifer via Histonet mailto:histonet@lists.utsouthwes

[Histonet] Animal Histology

Can anyone recommend a good book for processing animal tissue? Thanks, Jennifer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Changing decal question

Good afternoon, I would like to inquire what everyone's decal (RBD and/or Decal I) changing routines are. We are trying to determine if we should continue to change weekly, or based on how many specimens we put in it, or what the pH is. Any help is greatly appreciated, thanks! Jennifer Wooten,

[Histonet] ISH policies

Would anyone be willing to share their policies and procedures for ISH, specifically relating to kappa and lambda and CAP checklist guidelines? Thank you, Jen This message originates from the Yale New Haven Health System. The information contained in this message may be privileged and

Re: [Histonet] Sausage controls

The Journal of Histotechnology had an article regarding this years ago. Perhaps you can find it in the archives. Dr. Hector Battifora was the author. Jennifer -Original Message- From: Emily Horst via Histonet Sent: Wednesday, August 19, 2020 10:11 AM To:

Re: [Histonet] Cell block preparations

Acetic acid would work. Get Outlook for iOS From: Charles Riley via Histonet Sent: Thursday, January 16, 2020 8:55:21 AM To: Histo List Subject: [Histonet] Cell block preparations EXTERNAL SENDER- Exercise caution with requests, links,

Re: [Histonet] Canadian histology work

See CSMLS.org Get Outlook for iOS From: Alicia Murua via Histonet Sent: Thursday, August 15, 2019 1:56:48 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Canadian histology work EXTERNAL SENDER- Exercise caution with

Re: [Histonet] Policy bariatric patient for autopsy

Yes, there is a relatively new guideline in the checklist regarding performance of bariatric autopsies, and you need a policy at your institution **NEW** 08/21/2017 ANP.34160 Safe Handling of Bariatric Patients Phase II There are written procedures for the special handling of autopsies on

Re: [Histonet] We are Brainstorming on Promoting the Field of Histology and We Need Your Help!

You have my support and I believe the support of the CSH and the NSH. -Original Message- From: P Sicurello via Histonet Sent: Monday, October 15, 2018 10:03 AM To: jwwal...@rrmc.org Cc: HistoNet ; Bob Richmond Subject: Re: [Histonet] We are Brainstorming on Promoting the Field of

Re: [Histonet] Alcian Blue digested w Hyaluronidase - any alternatives?

The hyaluronidase is digesting out hyaluronic acid, a component found in connective tissue mucins. This reaction is used to differentiate connective tissue mucins from epithelial mucins. Hyaluronic acid and epithelial sialomucins will give the same staining results, both positive for alcian

Re: [Histonet] Sakura glas2

We use Permount and an aliphatic hydrocarbon clearing reagent. Jennifer Get Outlook for iOS From: Bitting, Angela K. via Histonet Sent: Wednesday, July 18, 2018 8:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura glas2

Re: [Histonet] Hazy/cooked cells in H

Is anyone using freezing spray? We had this problem and all cases were traced back to the same microtomist. She used the freezing spray excessively and was causing “freezer burn” to the GI biopsies. Jennifer Get Outlook for iOS From:

Re: [Histonet] New Bone Stain

Auto corrected on my phone. They were not decalcified. The counterstain was a brilliant red/fuschia in the osteoid. Get Outlook for iOS From: Jessica Riggleman Sent: Tuesday, July 10, 2018 1:16 PM To: Mac Donald, Jennifer;

Re: [Histonet] New Bone Stain

von Kossa will stain the mineralized bone black and a basic fuschin counter stain the osteoid will stain deep pink. Get Outlook for iOS From: Jessica Riggleman via Histonet Sent: Monday, July 9, 2018 1:17 PM To:

Re: [Histonet] Ethanol in tissue processing

We use reagent alcohol on the processor and for staining. Get Outlook for iOS From: Jamie Watson via Histonet Sent: Saturday, May 12, 2018 9:06:01 AM To: Histonet@lists.utsouthwestern.edu Subject:

[Histonet] CSH Annual Symposium

Please join us for the California Society for Histotechnology Annual Symposium in Anaheim. Workshops and registration information can be found at: http://californiahistology.org/events.html ___ Histonet mailing list

[Histonet] hand staining immunos

Hello, My dermatopathologist wants me to start hand staining immunos for MITF. I would really like some feedback about everyone's experiences/thoughts with manually hand staining immunos and especially with MITF. Pros and cons? I am concerned about the amount of time this will take for a tech