RE: [Histonet] De-differentiated tumor vs. Radiation effect

2009-09-23 Thread Kemlo Rogerson
they settled down to give a normal looking 'post menopausal smear'. Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail

RE: [Histonet] Separation Artifact

2009-09-22 Thread Kemlo Rogerson
incorrectly made up. Hope that helps. Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential

RE: [Histonet] air quality in the lab

2009-09-17 Thread Kemlo Rogerson
That's how often you have to turnover the water in a Koi Pond; 4 times and hour. Do you keep fish? Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have

RE: [Histonet] type of paraffin and polymer

2009-09-14 Thread Kemlo Rogerson
Ester Wax Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you

RE: [Histonet] FNA SLIDES

2009-09-01 Thread Kemlo Rogerson
is not to pull on the plunger but let it seep into the syringe. Too much blood and what little you have gets well diluted!! Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard

RE: [Histonet] Rat's lung

2009-07-24 Thread Kemlo Rogerson
Immerse it in water fully inflated and see how much water is displaced; is that Avogadro hypothesis or some other Greek dude? Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty

RE: [Histonet] histology for kids

2009-07-23 Thread Kemlo Rogerson
Depends on the age of the kids as I don't understand the term 'grade-school'. What I did for kids around 10 yesrs old or so was to go to the Butchers and get some Ox kidney, heart and liver. I prepared slides from them, took a microscope to let them see the structure and also took scapels for

RE: [Histonet] nuclear bubbling

2009-07-16 Thread Kemlo Rogerson
Sub optimal fixation and as Rene said; drying off at high temperatures. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: 15 July 2009 20:45 To: Histonet Subject: [Histonet] nuclear

RE: [Histonet] HTL

2009-07-15 Thread Kemlo Rogerson
), like the Clinical Scientists, obtain the MRCPath and then clinically lead a discipline. Only when we step from behind the skirts of the Medics will the sun shine on us. Does that help? Kemlo Rogerson MSc MIBiol CBiol DMS CSci FIBMS (I tried). -Original Message- From

RE: [Histonet] What percent of HTL's do not have a BS degree?

2009-07-15 Thread Kemlo Rogerson
The histology world doesn't look for well qualified workers they look for cheap labor (SIC). I have heard more than one pathologist state that a monkey can do our job. See my other post. The retort ought to be that a Histology BMS/ Histotech can do yours!! A honest Pathologist once told me that

RE: [Histonet] HTL

2009-07-15 Thread Kemlo Rogerson
2009 09:01 To: Kemlo Rogerson Cc: histonet@lists.utsouthwestern.edu; Shea's Subject: Re: [Histonet] HTL well said!! your statement: 'Only when we step from behind the skirts of the Medics will the sun shine on us' deserves dissection (pardon the pun) are we volutarily 'behind the medics or are we

RE: [Histonet] Help With Hemo Fading

2009-07-03 Thread Kemlo Rogerson
I agree.. Also you're not storing them in sunlight are you? Silly question I know. Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions

RE: [Histonet] signs of good perfusion

2009-06-30 Thread Kemlo Rogerson
That's cruel; how would you like it? Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential

RE: [Histonet] Sheep Lung

2009-06-25 Thread Kemlo Rogerson
Fixed or unfixed sheep lung? If fixed then you can perfuse with formalin from a header tank 1 metre above lung and then infiltrate with gelatine. Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace

RE: [Histonet] hood for manual special stains

2009-06-25 Thread Kemlo Rogerson
downdraught dissection tables and benches have back ventilation. Neither of these chemicals ought to be tolerated if you can smell them and exposure ought to be controlled. Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311

RE: [Histonet] microtome calibration

2009-06-03 Thread Kemlo Rogerson
Have you tried altering the angle of the knife? Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential

RE: [Histonet] Recycling Formalin for Fresh Tissue

2009-05-29 Thread Kemlo Rogerson
Why would you want to? Surely the time and energy needed to make sure that the recycled formalin was of the correct strength, was not infective and was at the correct pH and properly buffered would not make it cost effective. Bit like using a teabag twice; that's uncivilised!!! -Original

RE: [Histonet] cloudy formalin in vip tissue processor

2009-05-26 Thread Kemlo Rogerson
is a coagulant, non-additive fixative, normally). Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential

RE: [Histonet] Bone!

2009-05-26 Thread Kemlo Rogerson
As I say I don't have experience of preservatives but question if the effects of ethanol (70%) over a long time may also 'overfix' the bone? Anyway 70% ethanol has perfectly preserved me!! Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057

RE: [Histonet] cloudy formalin in vip tissue processor

2009-05-26 Thread Kemlo Rogerson
hehehe, thanks... Only got it completely the wrong way around didn't I? Freida is correct, I think! Kemlo Rogerson e-mail kemloroger...@nhs.net mailto:kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty

RE: [Histonet] cloudy formalin in tissue processor

2009-05-22 Thread Kemlo Rogerson
bloody or very proteinaseous tissue would have these labile proteins fixed which would then fall out of solution; from memory the cold makes it worse. Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace

RE: [Histonet] Bone!

2009-05-22 Thread Kemlo Rogerson
; my best advise is formalin but make sure that it is neutral and buffered. I think the effects of pH would be more damaging than the relatively 'soft' formalin fixation. Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob

RE: [Histonet] Gram AFB staining FNA smears

2009-05-07 Thread Kemlo Rogerson
may or may not have been fixed (although I concede air drying is a form of fixation) and the stains used on them have been shown to work. The logic that these techniques are interchangeable is not only flawed but (oxy)moronic. Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work

RE: [Histonet] New HTL Program!

2009-05-07 Thread Kemlo Rogerson
Congratulations from the UK. As you know we've had this in place for many years and resulted in the profession having a higher profile; I hope the rest will follow for you. Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension

RE: [Histonet] Destain Hematoxylon

2009-05-05 Thread Kemlo Rogerson
From memory I found that it was better to give a good water wash or go into bicarb after destaining with acid/ alcohol as the acidity impacted on the staining if you went from acid/ alcohol directly into haematoxylin again (if you wanted to restain it). Kemlo Rogerson e-mail kemloroger

RE: [Histonet] (canine) eosinophil IHC

2009-05-05 Thread Kemlo Rogerson
As already stated a good HE will, but a bad one will show them even better; wash most of the E out of the HE and the eosiniphils tend to let go of it last. Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194

RE: [Histonet] Lab Week-Histology Trivial or Fun Facts LONG OFF TOPIC

2009-04-15 Thread Kemlo Rogerson
I can only say Pathology needs more kids like you. I've no idea what your saying but it looks very interesting... Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems

RE: [Histonet] Liver (control tissue for PAS/D)

2009-04-15 Thread Kemlo Rogerson
I know this might be a stupid reply but as it's just for PAS/D can't you use animal liver? Why not pig's liver? Go to an slaughter house and get fresh pig's liver and bingo (I assume pig glycogen is the same as our's?)... You could fry the residual with onions, very nice. Kemlo Rogerson

RE: [Histonet] FREEZY spray

2009-04-08 Thread Kemlo Rogerson
Say thank you Uncle Kemlo!! Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged

RE: [Histonet] Controls needed!

2009-04-08 Thread Kemlo Rogerson
It's just a thought, can't you get the control from your Microbiology Lab? You just need to make a cell block out of the bacteria that they've grown; I'm not aware that Hpylori has to go through a human system before you can demonstrate it. Kemlo Rogerson e-mail kemloroger...@nhs.net

RE: [Histonet] Histology

2009-04-07 Thread Kemlo Rogerson
blocks of the same tissue, fixed for differing times and processed the way after being fixed with the same fixative; you can't go wrong. If I've been helpful then I apologise (g). Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension

RE: [Histonet] Re: Histonet Digest, Vol 65, Issue 14

2009-04-07 Thread Kemlo Rogerson
see you. Well I can cos people him replying to you and the craps at the bottom of the e-mail. My point so stop inflicting me on him, please! Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194

RE: [Histonet] Job opportunity in Cambridge, MA

2009-04-07 Thread Kemlo Rogerson
Try: Apply this rule after message arrives with Bernie Taulin in the senders address Delete it Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy

[Histonet] Getting rid of pests

2009-04-07 Thread Kemlo Rogerson
Or Bernie Taupin... Even. Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential

RE: [Histonet] Nice one Kemlo

2009-04-06 Thread Kemlo Rogerson
that Bernie, to stick at the end of your e-mails as a 'wise saying' (g). Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e

RE: RE: RE: [Histonet] IHC on paraformaldehyde-fixed

2008-12-08 Thread Kemlo Rogerson
Isn't petrol toxic? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Don’t be afraid to take a big step when one is indicated. You can’t cross a chasm in two small jumps. --Buckminster Fuller This e-mail is confidential

RE: [Histonet] can anyone tell me...

2008-11-12 Thread Kemlo Rogerson
So it is 10% formol alcohol in 70% alcohol; wonder what the other 20% is? Water? Alcoholic fixatives as a genre tend to overharden on standing but I guess fatty tissue would benefit. Why would anyone use something you don't actually know what it is composed of? -Original Message- From: