[Histonet] Miller's Elastin Stain

** Proprietary ** ** Reply Requested When Convenient ** Hi there, Does anyone use commercial Miller's Elastic Stain other than VWR Ref 35115S one ? If so I would be grateful if you could let me know which one you use Thanks in advance Malika Malika Benatti Specialist BMS Camelia Botnar

[Histonet] H1B Visa Sponsorship

I wonder if anyone can help or perhaps knows anyone who could. I am a UK trained and registered medical technologist and have specialised in Histology, with 16 years laboratory experience. I am fully registered as a Specialist Biomedical Scientist with the Health Professional Council (Similar

[Histonet] H1B Visa Sponsorship

I wonder if anyone can help or perhaps knows anyone who could. I am a UK trained and registered medical technologist and have specialised in Histology, with 8 years of experience. I am fully registered with theHealth Professional Council. I am looking for a H1B Visa Sponsorship opportunity

[Histonet] Olig2 (ab33427) on the bond max

staining and heavy background staining. Any suggestions would be appreciated! Thanks in advance Malika Malika Benatti Specialist BMS Camelia Botnar Laboratories Histopathology Department Great Ormond Street Hospital London WC1N 3JH Tel: +44 20 7405 9200 ext 5475 Fax: +44 20 7829 7875

[Histonet] Lyve - 1 on Bond Max

we seems to achieve is background staining with no real specificity at 1:100 H1(10) ; 1:100 H2(20) ;1:100 E2(10): 1:50 E1(30) ;1:50 H1(30); 1:50 H2(10) Any suggestion ? Regards Malika Malika Benatti Specialist BMS Camelia Botnar Laboratories Histopathology Department Great Ormond

[Histonet] RE: Coverslips

10123 SE Market St. Portland, OR 97216 booze...@ah.org Malika Benatti Specialist BMS Camelia Botnar Laboratories Histopathology Department Great Ormond Street Hospital London WC1N 3JH Tel: +44 20 7405 9200 ext 5475 Fax: +44 20 7829 7875 ben...@gosh.nhs.uk Weems, Joyce jwe...@sjha.org

[Histonet] H1B Visa Sponsorship

To all Histonet list members in Knoxville Area I am Specialist Biomedical Scientist fully trained in the United Kingdom with a BSc Hons in Biomedical Science and a Post Graduate Certificate in Cellular Pathology from the University of Westminster in London United Kingdom and currently looking

[Histonet] Glut1 antibody

___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 79, Issue 28 Malika Benatti Specialist BMS Camelia Botnar

[Histonet] Gordon Sweet Reticulin Stain problem on BMT Sections

) --- 30 sec 14. Wash in water. 15. Counterstain with neutral red. 16. Dehydrate, clear and mount. Any suggestions would be appreciated. Malika Malika Benatti Specialist BMS Camelia Botnar Laboratories Histopathology Department Great Ormond Street Hospital London WC1N 3JH Tel: +44 20

[Histonet] Alpha Dystroglycan Immuno

to 9:200 dilution immuno come out patchy not strong enough Any one on the histonet list use this antibody, if so at what dilution. any input would be really appreciated Malika Malika Benatti Specialist BMS Camelia Botnar Laboratories Histopathology Department Great Ormond Street Hospital

[Histonet] Chloroform Fume Monitoring

-online.com/ProductPages/Chloroform.htm Or is there any better alternative ? Malika Malika Benatti Specialist BMS Camelia Botnar Laboratories Histopathology Department Great Ormond Street Hospital London WC1N 3JH Tel: +44 20 7405 9200 ext 5475 Fax: +44 20 7829 7875 ben...@gosh.nhs.uk

Re: [Histonet] Dark background with Gomori's reticulin impregnation

Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Malika Benatti Specialist BMS Camelia Botnar Laboratories Histopathology Department Great Ormond Street Hospital London WC1N 3JH Tel: +44 20 7405 9200 ext 5475 Fax: +44 20 7829

Re: FW: [Histonet] studying for ASCP certification exam

Theory and Practice of Histological Techniques by John D. Bancroft and Marilyn Gamble Dr. you can probably get hold of a copy on Amazon for 1/2 price, even at full price it definitely worth every cents. On Wed, Apr 14, 2010 at 6:32 PM, Kim Merriam kmerriam2...@yahoo.com wrote: The NSH sells

Re: [Histonet] Communication when pathologist are not in same area as lab

Well our 5 pathologists, advance practitioner and SPR are just across the way from us, until last July all request were made personally by pathologist to staff and if there were any query with regard to the request made it was sorted then and then and it was pretty straight forward. But the good

Re: [Histonet] Communication when pathologist are not in same area as lab

...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Malika Benatti Sent: Wednesday, April 14, 2010 2:38 PM To: Scott, Allison D Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Communication when pathologist are not in same area as lab Well our 5

Re: [Histonet] Masson Trichome Staining: Can I use Fast Green?

acetic acid. Hope this help Malika Benatti Specialist BMS Camelia Botnar Laboratories Histopathology Department Great Ormond Street Hospital London WC1N 3JH Tel: +44 20 7405 9200 ext 5475 Fax: +44 20 7829 7875 ben...@gosh.nhs.uk John Kiernan jkier...@uwo.ca 09/04/2010 17:08 The slides

Re: [Histonet] Histobath

Hi Matt, Depending on the wax melting point which should be around 57 degree Celsius, your water bath should be set at 50 degree Celsius. Hope this help. Malika Benatti BSc MIBMS Specialist Biomedical Scientist Great Ormond Street Children Hospital London, WC1N 3JH UK Tel: (+44) 0207 4059200

Re: [Histonet] Histobath

Celsius, - the Cryo chamber at - 22 degree Celsius. Hope this help Malika Benatti Malika Benatti BSc MIBMS Specialist Biomedical Scientist Great Ormond Street Children Hospital London, WC1N 3JH UK Tel: (+44) 0207 4059200 Ext 5475 ben...@gosh.nhs.uk - Show quoted text - On Mon, Apr 5, 2010

Re: [Histonet] 72644.18148...@web111105.mail.gq1.yahoo.com

I am very confuse reading every email reply to this tread also I would be really grateful if someone could enlighten with regard to what is the comment practice in the US. Having been trained as a histotechnologist although we are call Specialist Biomedical Scientist in the UK, we cannot practice

Re: [Histonet] 72644.18148...@web111105.mail.gq1.yahoo.com

varies from province to province here. Our country wide qualifying system by the CSMLS is a fortunate anomoly that nobody wants to change because it works so well for us. I hope this explains a little. Bryan Llewellyn - Original Message - From: Malika Benatti malbena...@googlemail.com

Fwd: [Histonet] (no subject)

Dear Histonet list manager Is they anyway you could filter/block spam such as this one from be sent out to the Histonet list. Cheers Malika Benatti BSc MIBMS Specialist Biomedical Scientist Great Ormond Street Children Hospital London ... Smile it confuses people ... Begin forwarded

Re: [Histonet] cassette labels erased by processor

Hi there, I would suggest to use a pencil rather than a marker pen to label cassettes when processing cassette with the Sakura VIP , my lab had a number of the so called solvent proof pen on trial and have yet to find one that survive processing. Malika Benatti BSc MIBMS Specialist

Re: [Histonet] GMS stain

this help Best wishes Malika Malika Benatti BSc MIBMS Specialist Biomedical Scientist Great Ormond Street Hospital London, UK On Wed, Mar 31, 2010 at 7:46 PM, Perry, Margaret margaret.pe...@sdstate.edu wrote: We sometimes have problems with the stain if we use positive slides. Margaret

Re: [Histonet] cassette labels erased by processor

no risk of lab number not surviving processing.So if you lab as some spare cash to spend get few on trial in, az they are worth it. Best wishes, Malika Benatti BSc MIBMS Specialist Biomedical Scientist Great Ormond Street Children Hospital London ... Smile it confuses people ... On 1 Apr 2010

Re: [Histonet] Removing tissues from OCT for molecular analysis

studies. I need to separate tissues from OCT. Thanks, Katie On Mar 29, 2010, at 10:21 AM, Malika Benatti wrote: Do you mean removing OCT from tissue ? ... Smile it confuses people ... On 29 Mar 2010, at 13:55, Katie Crosby kcro...@cellsignal.com wrote: Hello, Can someone suggest

Re: [Histonet] freezing mouse heart tissue

You may found that excessive amount of saline could cause freezing artefacts, so dabe Try to embed heart sample on cryostat chuck directly, with OCT then freeze chuck on dry ice block directly as opposed to using liquid nitrogen. Another option, would be freezing tissue in hexane boiling tube,

Re: [Histonet] Paraffin Question

This will depend on the amount of blocks processed. I worked in places who used to change their VIP twice a week on Wednesday Friday, and other that would change processor solutions once a week, but as a rule, processor wax regardless of the make should ALWAYS be changed after a maximum of 5

Re: [Histonet] histo techs doing cyto prep work

specimen. Malika Malika Benatti Specialist BMS Camelia Botnar Laboratories Histopathology Department Great Ormond Street Hospital London WC1N 3JH United Kingdom Tel: +44 20 7405 9200 ext 5475 Fax: +44 20 7829 7875 On 26 Mar 2010, at 16:42, Kim Tournear wrote: Hi all, I'm curious

Re: [Histonet] H E QC

Hi there, HE QC should be carry out on every slides that are stained, if you are checking for the staining intensity, this will be carried out using a control slide on new batch of staining solution, commercial or house made. Batch number should be recorded, and slides labelled with batch

Re: [Histonet] coverslipper

We use the Leica CV5030 the advantage of it is that it can be attached to their Auto stainer providing continuous workflow as rack and coverslip 30 slides per run, without the need to physically transfer slides rack between the autostainer and coverslipper or used as a stand alone coverslipper if

Re: [Histonet] primate brain

At what thickness do you cut your sections ? For Human brain we cut section at room temperature moist in Molifex and cut sections at 7 µm for standard HE/ 14 µm for LFB. Not sure what is the melting point of the wax your use, but if it is around 57 oC you can safely raise the temperature of

[Histonet] Contamination..processor?

, or embedding but during processing but during processing and the tonsil tissue is definitely in the block I suggest that you use bio-wrap/tissue wrap. Cheers, Malika Malika Benatti Specialist BMS Camelia Botnar Laboratories Histopathology Department Great Ormond Street Hospital London WC1N 3JH

Re: [Histonet] Reticulin Stain

Cook if you don'g have access to any of these book you can download the staining protocol GORDON SWEET'S RETIChttp://library.med.utah.edu/WebPath/HISTHTML/MANUALS/RETIC.PDF Hope this help. Malika Malika Benatti Specialist BMS Camelia Botnar Laboratories Histopathology Department Great Ormond

Re: [Histonet] US HT

Janelle The HPC registration rules have changed but most of the info you need are available on the IMBS website http://www.ibms.org/ and the HPC website http://www.hpc-uk.org/ If you have a recognised Degree it should only be a matter of getting your Part One the of the HPC Registration to be

Re: [Histonet] number of slides

on all blocks Working in paediatric I have not seen a prostate of a breast for the past 6 years, but my old lab had between 4 and 6 blocks per prostate or breast tissue and were cutting HE at level 1/2/3 and picking up 4 USS between level for further IHC. Malika Malika Benatti Specialist

Re: [Histonet] UK Trained and fully HPC Registered Histotechnologist looking to relocate in the US

ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Malika Benatti Sent: Sunday, March 21, 2010 5:12 PM To: histonet

[Histonet] UK Trained and fully HPC Registered Histotechnologist looking to relocate in the US

To all Histotechnologist State Side, I am fully qualified in the UK in all aspect of the work in histology with 8 years post UK Health Professional Council (HPC) Registration (2 years in General Pathology, 6 years in Pediatric Pathology). I was wondering if someone on the Histonet list has