;
gently rinse in DW 1 min;
counterstain nuclei and all other steps with regular manner of your lab.
This solutions will not bleach DAB.
Sections will not detach from slides.
Hope this help.
--
Maxim Peshkov
Russia
Taganrog. mailto:maxim...@mail.ru
Charles, sea urchin spicules are contain a calcit, one of many forms of CaCO3.
Just use a soft decal agent, as 14% EDTA with pH 7.0. Check endpoint carefully
with weight loss
weight gain test.
--
Maxim Peshkov,
Russia,
Taganrog.
mailto:maxim
Here is very good free e-book:
http://www.leicabiosystems.com/fileadmin/biosystems/PDF/95.9890_Rev_C_Difficult_Blocks_and_Reprocessing.pdf
--
Maxim Peshkov,
Russia,
Taganrog.
mailto:maxim...@mail.ru
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I am searching the contact with Janet Minshew from Texas.
Janet, please, if it possible, e-mail for me directly: maxim...@mail.ru
--
Maxim Peshkov,
Russia,
Taganrog.
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Garrey,
We add to water bath 3-4 drops of Tween 20.
It is decrease the surface tension and small debrises of wax and tissues.
Also sections better become flat.
Adding of Tween 20 is necessary for all type of sections: for HE, HC, IHC.
Sincerely,
Maxim Peshkov,
Russia,
Taganrog
Julie,
Please, check the temperature around stainer or staining bath.
Dapaffinization will be poor or incompletely (as in youre case) in the cold
(under 18oC) room.
--
Sincerely,
Maxim Peshkov,
Russia,
Taganrog.
You wrote:
Hi,
I made slides of paraffin-embedded mouse small intestine (Swiss
Laurie,
The slides must be pre-coated with poly-L-lisine or any other type adhesive,
suitable for IHC.
IHC is done usually for a several markers. Do you have a several HE slides?
Sincerely,
Maxim Peshkov
Russia,
Taganrog. mailto:maxim...@mail.ru
individually sections will have own specific thickness.
Sincerely,
Maxim Peshkov,
Russia,
Taganrog.
mailto:maxim...@mail.ru
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adhesive.
Histonet people's teached me for fatty tissues to use two main rules:
1. Thicknes slices for processing no more than 2 mm
2. Fixation in 10%NBF at least 24-48 hours.
Maxim Peshkov,
Russia,
Taganrog. mailto:maxim...@mail.ru
Dear histonetters,
I want to add, that it is very important
to use Schiff's reagent in lab environment
without any formaline vapours.
--
Sincerely,
Maxim Peshkov
Russia,
Taganrog.
mailto:maxim...@mail.ru
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Tasha,
1. Try 10% Potassium Iodide (KJ) for 5-10 minutes, spot will turn to yellow as
lemon,
then apply 10% Sodum Thiosulfate, wipe it and then talc powder and dry.
2. 15% sodium chloride with some drops hydrochloric acid, then apply 10% Sodum
Thiosulfate,
wash and dry.
Maxim Peshkov,
Russia
Tim,
HM340 + STS now successfully uses in
many Russians hisology labs and techs
loves them.
STS have the one disadvantage: is need
to much time for cleaning and
maintenance vs waterbath.
Also it is necessary some skills for
using it.
Sincerely,
Maxim Peshkov,
Russia,
Taganrog
.
Series 2125 is the same as Microm 325.
I do not try Leica Cool Clamp.
Hope this help.
Maxim Peshkov
Russia,
Taganrog.
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Lucy,
As well 70% EtOH we use 70% IPA for
long storage formalin fixed tissues.
Maxim Peshkov,
Russia,
Taganrog. mailto:maxim...@mail.ru
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.
Maxim Peshkov,
Russia,
Taganrog.
Original message---
From: Johnson, Kevin kjohn...@med.miami.edu
To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu
Sent: Friday, February 8, 2013 1:00 PM
Subject: [Histonet] Could these samples have been saved?
Dear all,
During an overnight
this
and/or pathologist not like the slides.
If this supervisor can do such work at least one
week with great results, then I want to learn this
and many other things from he.
Sincerely,
Maxim Peshkov
Maxim mailto:maxim...@mail.ru
solution after workday.
Never any metallic film on surface.
Before hematoxyline we rinse rack with slides in DW 1
minute and drain excess water.
Always very good results.
Sincerely,
Maxim Peshkov
Russia,
Taganrog.
You wrote:
We are currently using a vintage solution,
and the manufacturer states
Dear Histonetters!
Can anybody to advise, what type automatic knife sharpener
we can buy in Europe (Germany) instead Leica SP9000,
which now is unavailable for sale? What vendors?
We do not like a Shandon Autosharp 5 type sharpener.
Maxim Peshkov
Russia,
Taganrog.
mailto:maxim...@mail.ru
and lab aides.
Sincerely,
Maxim Peshkov,
Russia,
Taganrog. mailto:maxim...@mail.ru
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as usual and
you will see clear and crisp counterstain.
If hematoxyline will be to much, then you can
differentiate it in 0.25% HCl 5-10-15 dips, then wash
buffer.
Hope this help.
Maxim Peshkov,
Russia,
Taganrog.
From: Megan French megan.fre...@mcri.edu.au
Subject: [Histonet] DAB haematoxylin
I am sorry, that uses histonet for personal goals.
Dear Rene!
Last 3 my answers for you are returned back to me.
Your e-mails I got successfully.
Support service of mail.ru wrote that problem is at
yahoo.com server. Please check your postmaster service.
Here is my post-address:
Maxim Peshkov,
ul
and gentle. Mineral
oil is absolutely not toxic for personnel.
Transitions between IPA, MO and paraffin are very
gentle.
Please let me know about results.
If you need any more details please contact to me.
Best wishes,
Maxim Peshkov,
Russia,
Taganrog. mailto:maxim...@mail.ru
Dear Histonetters!
Can anyone send for me the last full pdf-version on
CAP questionnaire for AP and IHC lab, please?
I can not able to download it from CAP website.
Maxim Peshkov,
Russia,
Taganrog.
mailto:maxim...@mail.ru
Margaret:
We uses 1% aqueous neutral red as red counterstain
with very good results.
Do not forget rinse slides at 10-15 secs in DW
with 1-2 drop of glacial acetic acid before neutral
red and after this, because this dye have red color
at pH 6.
Sincerely,
Maxim Peshkov,
Russia,
Taganrog
. 5-5.5. At reduction pH red conterstain will
look as you have seen.
Maxim Peshkov,
Russia,
Taganrog.
mailto:maxim...@mail.ru
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Patsy:
Chemically, silver nitrate deposites can be
reduced onto the slides by 0.5% potassium ferricyanide.
I am not sure that it may work onto living skin.
Sincerely,
Maxim Peshkov,
Russia,
Taganrog.
mailto:maxim...@mail.ru
.
-Original Message-
From: Maxim Peshkov [mailto:maxim...@mail.ru]
Sent: Tuesday, March 23, 2010 11:43 AM
To: pru...@ihctech.net
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] help (UNCLASSIFIED)
Patsy:
Chemically, silver nitrate deposites can be
reduced onto the slides by 0.5
was not react.
Please, anyone help for us which antibodies, detection
system, manufacturers and vendors need to use?
Sincerely,
Maxim Peshkov,
Russia,
Taganrog.
mailto:maxim...@mail.ru
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not a
possibility to buy it.
Sincerely,
Maxim Peshkov,
Russia,
Taganrog. mailto:maxim...@mail.ru
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Dear colleagues!
My pathologists asked me about books for myocardial
biopsies (Histopathology of Myocard;
Endomyocardial Biopsy).
Can you recommend any good books for pathologists and techs
about these issue, please?
We are new on this field.
Thanks you for advise.
Sincerely,
Maxim Peshkov
Rene,
I am sorry, that I need call to you through Histonet,
but, it seems that yahoo-server have some troubles
with my mail-delivery.
I got next message now:
This is the mail system at host fallback2.mail.ru.
I'm sorry to have to inform you that your message could not
be delivered to one or
excluded ethanol as dhydratant.
If you need our processing schedule, please let me know.
All the best,
Maxim Peshkov,
Russia,
Taganrog. mailto:maxim...@mail.ru
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Sandra:
The volume of water under the section onto slide are
very small for microwave heating and this water
cannot be heated adequately. Paraffin is transparent
for microwave and therefore instead drying of slides
you will have a damaged magnetron.
Never dry paraffin sections in MWO!
Maxim
answer to me privately.
Thank you and I only hope that somebody can help us!
Sincerely,
Maxim Peshkov,
Russia,
Taganrog.
mailto:maxim...@mail.ru
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Dear colleagues!
One friend of mine can not able to find full text of the article:
Karlsen F, Kalantari M, Chitemerere M, et al.
Modifications of human and viral deoxyribonucleic
acid by formaldehyde fixation. Lab Invest 1994;71:604–11.
Can anyone help to us?
Many thanks.
Maxim Peshkov,
Russia
Angela:
We stains our cytology slides for Gram and AFB with
the same reagents as histology. Only misses dewax and
final dehydration, clearing and coverslip steps.
Results are like both, the pathologists and
cytopathologist.
Maxim Peshkov,
Russia,
Taganrog.
mailto:maxim
We also addes 4-5 drops of Tween 20 in waterbath.
Human eye's sections have not any wrinlkes.
Maxim Peshkov,
Russia,
Taganrog.
--- On Wed, 3/4/09, Perry, Margaret
From: Perry, Margaret margaret.pe...@sdstate.edu
Subject: [Histonet] problem eyes
To: histonet@lists.utsouthwestern.edu
histonet
Jackie,
Lipofuscin in FFPE sections is good stain with
Oil red O or similar fatty dye (Sudan III, IV)
with following mounting in glycerol-gelatine.
Maxim Peshkov,
Russia,
Taganrog.
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