If you’re doing online retrieval/deparaffinization, try deparafinizing offline.
I found the paraffin we loved had was higher in polymers and resulted in the
tissue being prone to lifting. As soon as we started deparafinizing BEFORE
putting the slides on the instrument, we had greater (not perf
From the vendor perspective, we have definitely seen an increase in
implementation of our processors. Going to the xylene-free, isopropanol
clearing approach has lead to converts because the process is more gentle
(xylene can harden dense tissues and over clear small specimens). Before
coming
Unfortunately, there are no “best practices” for room conditions that I can
find. The only real standard is to follow the manufacturer’s guidelines for
reagents AND equipment. There is a reference to ideal ranges on the FDA
website, but it’s specifically refers to clinical labs. I look forward
We baked our slides in our IHC oven on metal trays (for cytology) overnight so
we could file them. 15 minutes wouldn’t have worked, but maybe they are using
a hotter oven. As for the air pockets, the baking won’t solve that. This
usually (but not always) happens when the ratio of mounting med
I don’t know the brand, but they are called paratrimmers. Multiple vendors
carry they such as Newcomer or Mercedes. My techs loved them!
From: Jay Lundgren via Histonet
Sent: Wednesday, March 1, 2023 6:04:37 PM
To: histonet
Subject: [Histonet] Who sells that bl
This is so hard to unpack everything that could be wrong with this situation.
The weights the lab assistants take would lead me to the conclusion that this
would be part of grossing, because it is reportable. It’s such a slippery
slope when you start delegating tasks associated with high compl
We included random reviews along with any discrepancies and how they were
handled. If you look through the CLIA brochure for competency assessments, it
defines all of the required elements. You need to cover each of the points
mentioned somehow. I included the link :)
https://www.cms.gov/regul
Hello
As a matter of interest, what processes do labs use for the discarding of
formlin, xylene and alcohol laboratory waste?
Regards
Michelle
From: Greg Dobbin via Histonet
Sent: Monday, April 4, 2022 2:14 PM
To: nancy.schm...@mercyhealth.com; histonet@lists.utsouthwestern.edu
Subject: Re
Hello
I would like to know if any histology laboratory stores their fixed specimens
this way and what instrument do you use?
Regards
Michelle
Sent from Mail<https://go.microsoft.com/fwlink/?LinkId=550986> for Windows
Disclaimer - University of Cape Town This email is subject to UCT polici
Hi All,
We have numerous pipettes from facilities we have acquired over the
years. We are considering donating them. If you know of causes that
would benefit could you please let me know.
Thank you!
Michelle
--
Best of all things to you,
Michelle Jamison
//
Tel : +1.435.752.6011 Ext
? Thanks much for any input, especially if you have a recipe
you would like to share for artificial matrix!
Michelle
--
Best of all things to you,
Michelle Jamison
//
Tel : +1.435.752.6011 Ext. 435.227.1474
m.jami...@elitechgroup.com • www.elitechgroup.com
<http://www.elitechgroup.
!!
--
Best of all things to you,
Michelle Jamison
//
Tel : +1.435.752.6011 Ext. 435.227.1474
m.jami...@elitechgroup.com • www.elitechgroup.com
<http://www.elitechgroup.com/>
irs in the
processor?
I currently coverslip with Eukitt mounting medium, is this compatible?
Anything other advice you can offer or things you have discovered during your
switch?
Thanks!
Michelle (Shelly) Aono
Research Associate II
107B/124 Greene Hall
Auburn University, D
effect? If so, what do you
do for that? Last oneif you do dehydrate with alcohol, would you
ever consider air dehydration instead?
Thanks for your input,
Cheers!
Michelle
--
Best of all things to you,
Michelle Jamison
//
Tel : +1.435.752.6011 Ext. 435.227.1474
m.jami...@elitechgroup.com
Hi All, I am from industry. We are interested in developing an
instrument that stains then prepares microscope slides to be
coverslipped. If you do coverslip, what substitute do you use instead of
toluene to go to mounting media? Thank you! Michelle
--
All the best to you,
Michelle Jamison
Hi All, Do you know of a similar list serve that is for Cytologists?
Thank you,
Michelle
--
All the best to you,
Michelle Jamison
/Bio Research Associate /
/ELITechGroup Inc. / • 370 W. 1700 S. • Logan Utah • USA
m.jami...@elitechgroup.com <mailto:m.jami...@elitechgroup.
City
Utah area.
Thank you!
Michelle
--
All the best to you,
Michelle Jamison
/Bio Research Associate /
/ELITechGroup Inc. / • 370 W. 1700 S. • Logan Utah • USA
m.jami...@elitechgroup.com <mailto:m.jami...@elitechgroup.com> •
_www.elitechgroup.com <http://www.elitechgroup.
Hi All
I am interested in knowing what samples you all are staining with Gills
or Harris or H&E staining.
I am interested in both liquid and paraffin preparations.
Thank you!
Michelle
--
Michelle Jamison
BioEngineering
Elitech Biosystems
370 West 1700 South
Logan UT, USA
84321
435.227.
I agree with Toysha. I have used both instruments, and the only consistency in
staining I could get with the Ventana was inconsistent staining. The DAKO is
more user friendly, and stains can be completed if the instrument goes down or
stops in the middle of a run.
Michelle R. Dragoo, MBA, HT
Hi Fred,
We use the ones from Thermo Fisher and are quite happy with them.
Michelle R. Dragoo, MBA, HT (ASCP)
Supervisor, Histology
Northwestern Medicine Central Dupage Hospital
25 North Winfield Road, Winfield IL 60190
O: 630.933.6748
michelle.dra...@nm.org
-Original Message
We are the same as Tim. Typically we try to get things out within 24 hours,
that said, it depends on how old the case is, does it need recuts etc. etc. I
think your current time of 48 hours sounds quite reasonable.
Michelle R. Dragoo, MBA, HT (ASCP)
Supervisor, Histology
Northwestern Medicine
Just wanted to let all Histoneters know there is a job posting for a certified
HT or HT certification eligible person at Hamilton Medical Center in Dalton GA.
Day shift, no holidays, no weekends, no call.
See Hamiltonhealth.com under Careers.
___
what I've read in the archives for immunohistochemistry?
Thanks so much.
Michelle B. Titunick
PhD Candidate, Anatomy
The Pennsylvania State University College of Medicine
mbt...@psu.edu
(973) 715-9831
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the crack? If it is
peripheral and localized on one side, you may want to check the angle of the
blade. If it is throughout, then you may want to check if block has totally
sink in sucrose prior to sectioning.
Let me know if you have any additional question.
Michelle
> On Feb 17, 2017,
Hi,
The reactivity of the antibodies depend on the epitope/immunogen of the
antibodies. I would advice to check % homology of the eptiope/immunogen to
that of chicken to know if the antibodies work for on chicken. High
homology means it is very much likely that it will work.
Thank you
Michelle
onate, recycle such items? Do people even use
these anymore? I would hate to have them thrown out.
Thanks!
Michelle (Shelly) Aono
~~~
Research Associate II
107B/124 Greene Hall
Auburn University, Dept of APP
Auburn, AL 36849
(334) 844
Good charged slides or subbed slides will work.
In regards to the fixation, I personally find the morphology with 4% PFA O/N
followed by 30% sucrose better than doing them simultaneously.
Michelle
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metimes it help.
Good Luck
Best,
Michelle
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I just wanted to say thanks to all who assisted me in my goal of getting a
lab CLIA certified. They had their inspection and just before Christmas
they received their CLIA number. They got through their inspection with
zero deficiencies! :)
Thanks again,
Michelle
e the link directly to the posting:
http://jobsearch.childrens.com/us/en-us/Job-Details/Sr-Medical-Tech-Histology-Services-Job/Dallas-Texas/XjdP-jf564-ct101520-jid52860517
Or, please visit www.childrens.com and look for the Sr. Med Tech in Allied
Health.
Michelle Lamphere
Senior Tech, Histolog
processing?
Also, are there microwave processors on the market that have safety measures in
place to prevent this sort of error?
Michelle Lamphere, HT(ASCP)
Lead Tech, Histology
Department of Anatomic Pathology
1935 Medical District Dr.
Dallas, TX 75235
214.456.2318
Please consider the environment
us with pricing of interfaces. Everything is
based on your current equipment, not the equipment that you have when the
project actually goes live either. If you want to know more, feel free to
contact me directly
Michelle Lamphere
Senior Tech, Histology
Anatomic Pathology
O: 214.456.23
, etc.
Michelle Lamphere
Senior Tech, Histology
Anatomic Pathology
O: 214.456.2318 | Fax: 214.456.0779
E: michelle.lamph...@childrens.com
1935 Medical District Drive | B1.06 | Dallas, Texas 75235
Message: 2
Date: Fri, 29 May 2015 14:23:00 -0400
From: "Blazek, Linda"
To:
? If you are currently using a
program, what do you like? And what are the limitations? We are currently in
the process of implementing AB&T with Cerner, but will be moving to Epic in the
next couple of years.
Thank you in advance
Michelle Lamphere
Senior Tech, Histology
Anatomic Pa
have guinea pig
inner ears I could have... If you have ever sectioned inner ear I would love
to hear from you! Orientation seems to be critical and the one attempt we did
on dog IE was a miserable failure. Thanks for any and all help!
Michelle (Shelly) Aono
~~~
Research
ice not to see those messages.
Michelle
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michelle
Sent: Saturday, February 7, 2015 10:01 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] it happened
Does anyone have a clue as to why I keep getting a "foreign" reply to every
email I send to histonet? Is the group seeing the same foreign message I am
seeing? Am I the only one getting this message?
Michelle
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It seems every time I reply to a histonet email, it is followed up with a
foreign character email. I have no idea why this could be happening, if, in
fact it is related. I am just testing to see if a newly composed email will
do the same thing?
Thanks,
Michelle
ource. :)
Michelle
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Have some cloudy Fisher Superfrost plus slides. Sometimes they're cloudy even
from boxes that are still in the plastic wrapping. Does the wisdom of the
collective histonet think these slides are okay to use or should be tossed?
Thanks!
Michelle (Shelly) Aono
~~~
Res
research on the net, but
it can get so convoluted and confusing, I was hoping you might be able to
help me navigate some of the waters.
Thanks, in advance,
Michelle
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http
We have full and part time openings at Children's Health in Dallas, TX.
Positions require qualification for High Complexity Testing. Please contact me
directly to inquire.
Michelle Lamphere
Senior Tech, Histology
Children's Health
214-456-2318
michelle.lamph...@childrens.com
Pleas
antibody and detection system for longer time
as the tissue is thicker.
PS: You will not be able to handle free floating section that is thinner
than 25um.
Let me know if you need more information.
Michelle
michang2...@gmail.com
Date: Wed, 20 Aug 2014 13:06:54 +
From: Barbara Tibbs
the Omnis with
you as we are discovering them.
Michelle Lamphere, HT(ASCP)
Lead Tech, Histology
Department of Anatomic Pathology
1935 Medical District Dr.
Dallas, TX 75235
214.456.2318
Message: 3
Date: Wed, 29 Jan 2014 17:17:16 -0800
From: joe joe
Subject: [Histonet] Dako's Omnis
To: &quo
Are there any histology programs or school in the Atlanta, GA area? If not,
what would be a good place to start (in Atlanta) for somebody who wants to
become a histotech?
Michelle Lamphere, HT(ASCP)
Lead Tech, Histology
Department of Anatomic Pathology
1935 Medical District Dr.
Dallas, TX
We use Richard-Allan Paraffin Type 9. Embed nice and cuts beautifully for our
lab.
dermatology
CONSULTANTS
skin. care. experts.
NOTICE OF CONFIDENTIALITY: The information in this email may be privileged and
confidential and protected from disclosure. If the reader of this message is
not
We put all 3 levels on one slide as well.
Michelle R. Dragoo, MBA, HT (ASCP)
Histology Coordinator
Alverno Provena Hospital Laboratories
Presence Saint Joseph Hospital - Elgin
77 N Airlite St | Elgin, IL 60123
T: 847.695.3200 x5309 | F: 847.888.3318
michelle.dra...@presencehealth.org
Good Day Histoland!
Would anyone like to spend some of your winter in a tropical paradise? I have a
temporary HT position in the US Virgin Islands. Please email me if you are
interested. You must be ASCP certified.
Michelle Moore
Histopathology Supervisor
Schneider Regional Medical Center
St
We are writing path #, date of birth & patient initials as identifiers.
Michelle
From: Jean Wood
To: "histonet@lists.utsouthwestern.edu"
Sent: Tuesday, July 30, 2013 2:37 PM
Subject: [Histonet] Two Patient Identifiers on slides
Hell
Good day Histoland!
Can anyone that is using a formalin substitute that is happy with the quality
of fixation please share!
Thank you.
Michelle Moore
SRMC
St. Thomas USVI
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you do not have the
alcohols on the stain line either = huge cost savings all the way around, not
to mention the healthier work area!
Michelle Moore
SRMC
St. Thomas, USVI
From: "e...@pigsqq.org"
To: histonet@lists.utsouthwestern.edu
Sent: Wednesday, Jul
Lab
checklist that did not apply to ANP. Kinda shocking, I know.
It does not mean that we scrapped our entire competency program, we simply
removed some of the six elements.
Michelle Lamphere
Senior Tech, Histology
Anatomic Pathology
Children's Medical Center
O: 214.456.2318
http://montrealbarbell.com/nnlxwbn/cgia/pfstr/ucw.htm
Best regards, Michelle Moore
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. As specimens are
entered in EPIC, they will show up on the monitor in both the OR and histology.
As we receive them, they will drop off the list. Both departments will then
have a running log of every specimen from the OR. At least that is the
theory.
Michelle
-Original Me
Has anyone tried or have a Wave RPD system from Celerus Diagnostics in their
pathology lab? If yes, do you stain immunohistochemisrty on Mohs and paraffin
embedded slides? Would you suggest this instrument for a small lab? Also what
are the pros and cons using the Wave RPD system performing pa
rped and pulled off and pulled the
tissue sections with it. I am sure they have made improvements since then, but
made the lab very leery of using the tape.
Michelle M Lamphere, HT (ASCP)
Senior Tech, Histology
Children's Medical Center
1935 Medical District Drive
Dallas, TX 75235
Office :21
specimens make it
to histology?
Michelle M Lamphere, HT (ASCP)
Senior Tech, Histology
Children's Medical Center
1935 Medical District Drive
Dallas, TX 75235
Office :214-456-2798
Histology: 214-456-2318
Fax: 214-456-0779
Please consider the environment before printing this e-mail
This e
Does anybody in the DFW area have a vendor or company (other than the
manufacturer) that they would recommend to service their histology equipment?
Annual PMs, emergency repairs, etc...
Michelle M Lamphere, HT (ASCP)
Senior Tech, Histology
Children's Medical Center
1935 Medical District
I really appreciate any insight or advice you can offer. Thank you!
Michelle M Lamphere, HT (ASCP)
Senior Tech, Histology
Children's Medical Center
1935 Medical District Drive
Dallas, TX 75235
Office :214-456-2798
Histology: 214-456-2318
Fax: 214-456-0779
Please consider the environment
We do this on a fairly routine basis with our GI biopsies and have found it to
be most effective. Leaving them in the warm retort exposes the tissue to heat
longer than normal and could cause some problems.
On Feb 12, 2013, at 1:12 PM, Robert Schoonhoven
wrote:
> All you need to do is have t
I would love to hear any feedback that you get from this query.
Michelle M Lamphere, HT (ASCP)
Senior Tech, Histology
Children's Medical Center
1935 Medical District Drive
Dallas, TX 75235
Office :214-456-2798
Histology: 214-456-2318
Fax: 214-456-0779
Could I get the opinions of any
VoiceBrook is an awesome pathology voice recognition system! We use Meditech
and it does not require an interface.
Michelle
From: Ann Specian
To: histonet@lists.utsouthwestern.edu
Sent: Tuesday, January 15, 2013 6:37 PM
Subject: [Histonet] Voice Recognition
advance.
Michelle M Lamphere, HT (ASCP)
Senior Tech, Histology
Children's Medical Center
1935 Medical District Drive
Dallas, TX 75235
Office :214-456-2798
Histology: 214-456-2318
Fax: 214-456-0779
Please consider the environment before printing this e-mail
This e-mail, facsimile, or l
One thing to remember when addressing quality issues in the lab is to find a
root cause. If the same mistakes are being made over and over by both techs,
then that isn't a personnel problem that can be fixed with rewards/punishment.
That is a process problem. You need to review you processes and
vious date if the machine is run for a
few days in a row and then taken off service for a few days. If you are not
careful, a delayed start becomes an immediate start as the machine tries to
backtrack to the previous date.
Michelle M Lamphere, HT (ASCP)
Senior Tech, Histology
Children's
Has anyone done manual IHC for Melan-A? If yes, who help set up the manual
protocol?
Thanks,
Michelle
Michelle M MacKinnon HT, (ASCP)
Histology Supervisor
Dermatology Consultants. PA
1215 Town Centre Drive, Suite 200
Eagan, MN 55123
Phone: 651-255-3462
Fax: 651-255-3461
combatible with
Meditech..ughhh!
Thanks in advance for any help or direction!
Michelle Moore
Schneider Regional Medical Center
9048 Sugar Estate
St. Thomas Virgin Islands 00802
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At what thickness are you cutting the subject tissue? I have had challenges in
the past with false negatives (on subject tissue not controls) turned out we
were cutting special stain slides too thin. Bump it up a notch or two & see if
that helps :)
From: amand
Hello all
If tissue (from autopsy cases) has been fixed in 10% formal saline,
will there be a difference in the stained section if:
processed in methanol
processed in ethanol
Thanks
Michelle
Michelle Perrins
Chief Medical Technologist
Forensic Pathology Services
Division Forensic
reduction from $83.01/100
slides down to 4 pennies! My tech's are breathing in much deeper :) and excited
to continue becoming as green as possible!
Best Regards-
Michelle Moore
Histopathology Supervisor/Medical Examiner Ofc
Schneider Regional Medical Center
St. Thomas, USVI,
evant scientific and technical aspects
* Ability to supervise other scientists when necessary.
Preferred qualifications:
* HT or HTL certification
* Ventana expertise
Michelle Broome, MBA, HTL(ASCP)
Laboratory Manager
PCS Discovery and Investigative Pathology
Novartis Institute
If interested, email resume to mgiresu...@gmail.com. Must be qualified to
perform gross and be able to perform all basic histology duties.
Sent from my iPad
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We are currently have a histology position open at Children's Medical Center in
Dallas. This position requires working knowledge of all aspects of histology
including immunohistochemistry. Associates degree in a science discipline
preferred, HT or HTL (ASCP) or equivalent or certification elig
compliance.
Michelle Lamphere, HT (ASCP)
Lead Tech, Histology
Children's Medical Center
1935 Medical District Dr
Dallas, TX 75235
tel 214.456.2318
michelle.lamph...@childrens.com
Please consider the environment before printing this e-mail
This e-mail, facsimile, or letter and any files or attach
Hi Histonetters! Does anyone have a protocol for fixing, processing,
sectioning brown adipose? I don't have any experience with adipose so a
detailed description would be fantastic. I think the tissue will be coming
from squirrels.
Thanks!
___
His
Histology position available in Dalton, GA. Full-time; dayshift, hours may
vary, no weekends, no holidays.
Please visit HamiltonHealth.com and click on the Career section.
Thanks,
Michelle Massey, HT (ASCP)
Tumor Bank Coord. | Histology Team Leader
Hamilton Medical Center
1200 Memorial Drive
alidation itself, we also need to validate IHC
use on the Peloris, per one of our pathologist's. How many antibodies,
controls or panels should we be doing to satisfy the 'validation' process for
IHC?? Any information will be helpful, thank you so much!!
Michelle Wahlsten, HT
My daughter is doing a paper on "repetitive motion injuries" I thought what
better than the AO Microtomes, if anyone has any info or can suggest any web
sites with articles on ergonomic issues histo techs have experienced over the
years we would greatly appreciate it, Thank
end of the month we will also have available
4. Embedding center - SHANDON Histocentre2 (still in use)
If interested please send me an offer.
Michelle Aloni MS HTL Research Specialist
USC Keck School of Medicine Los Angeles, CA
deparaffination
and only dehydration for our immunos.
If any one has experience with the TBS unit, or even better, used both of
them, please let me know what do you think.
I would appreciate this very much
Michelle
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o and
understanding client/customer needs and then delighting the
client/customer by exceeding service and quality expectations.
Please contact Michelle Hardin at mhar...@carisls.com for additional
details. Thanks!
Michelle Hardin
Corporate Recruiter
Caris Life Sciences
502-418-9447
www.carisl
We are seeking an experience Histotech for our lab in Phoenix, AZ. We
are on the cutting edge in the biotechnology industry and are looking
for dynamic lab professionals to join our team. If you would be
interested, please contact me for details!
___
Hi
tremendously. I also had a removable plastic cast to restrict movement.
Luckily the work was done by another lab and I now have extra staff.
The pain only comes back if it gets very cold, otherwise my wrist is
fine now.
Michelle Perrins
Chief Medical Technologist
Forensic Pathology Services
Division
I was cutting some bone/joint tissue and noticed that the cartilaginous
portion was concave/indented, instead of flush with the rest of the
block surface. Even as I continued to cut that portion always seemed a
little sunken into the block face and all the sections crumbled. I
didn't seal the blo
Michelle Schwab-MacDonald HT(ASCP), QIHC
Supervisor IHC, Neuropathology, & Special Stains
Education Coordinator Histopathology
Massachusetts General Hospital
Warren 501D
55 Fruit Street
Boston, MA 02114
617-643-4361
The information in this e-mail is intended only for the person to whom i
Hello
I would like your views on which provides the better result regarding
fat stains.
1. fat stains (Oil red O, etc) on frozen sections
2. osmium tetroxide on formalin fixed tissue
I know that osmium is nasty to work with but besides that does it
produce a better result?
Thanks
Michelle
blocks down personally for a visit to the Caribbean! I am in need
of fungus, AFB and H. Pylori. Any help would be fantastic and very appreciated!
Michelle Moore
Histopathology/Cytology Supervisor
Schneider Regional Medical Center
9048 Sugar Estate, ST. Thomas, US VI 00802
340-776-8311x1047
mlmo
Sarah,
I worked this antibody up using normal colon. Uterine cervix, lung, and
gallbladder (all normal) should also stain nicely.
Michelle Mathews, HT(ASCP)
Research Specialist
TPL
Does anyone know what the positive control for MET is?
Thanks
Sarah Goebel, BA, HT(ASCP
rs ago when I parted with my Histo lab and I am not current on
the latest regulations, but as far as the technical part... there shouldn't
be a problem.
Michelle Aloni MS, HTL ASCP
USC Keck School of Medicine
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THANKS! It worked J
Michelle
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I would also be interested to know that.
I am not even familiar with Welcan units.
Would someone please elaborate?
Michelle Aloni
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can share with me. You can just scan and e-mail your
pricelists if this is easier, and please mention the city and state of your
location.
If you would rather fax it, please write back and I will give you our fax
number.
Have a nice day and thank you ahead of time
Michelle Aloni
helpful thought
Michelle
USC Keck School of Medicine
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same time as the other species so it's not variations
in staining time, solution age, etc...
Has anyone else seen this effect?
Any ideas on how to fix this and why this is?
Species proteoglycan compositional differences?
Thanks!
Histology Newbie,
:) Shelly
~~~
Michell
ls and the longer you wait
the larger the crystals = have to repeat it all over again J
4. To clean the glassware, spray All Purpose Cleaner, use a brush and
warm water
Michelle
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Aqua Mount
Never had any problems. Normal muscle will have very, very tiny droplets. We
used it on rodent muscle, but it should work the same for human.
Good luck
Michelle Aloni
Research Specialist
USC Keck School of Medicine
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Our microtome is old and no longer serviced by Leica. Does anyone know
if independent contractors can do routine maintenance on old microtomes
and how I might find them? Thanks.
Shelly
~~~
Michelle (Shelly) Aono
Histology Technician IV
CVM & AU Staff
Advisory Cou
Absolutely not!!! In California.
Safety office picks it up and dispose of it.
Michelle Aloni
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Once you mix your stock solution with water, it is good for only about an
hour. Then you have to discard it.
Michelle Aloni MS HTL (ASCP)
USC Keck School of Medicine
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aintenance for histo equipment and how much
would this be.
Beside all the old stuff we also have a new Leica Bond, which we would like to
cover with someone different then Leica.
Please contact me off the list.
Michelle Aloni
Research Specialist
macve.
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