[Histonet] S100

Our S100 is not working very well. I think it's too old. Can someone recommend an S100 antibody that works well in dog? It would be really nice to find a good monoclonal instead of the polyclonals. We are looking for schwannomas, astrogliomas, malignant melanomas and their metastases. Marga

[Histonet] antibody diluent

I am looking for a less expensive antibody diluent and would like some opinions on which companies are best . Are there differences between diluents when it comes to background staining? Margaret Perry HT(ASCP) Veterinary & Biomedical Sciences Department North Campus Drive Box 2175 South Dakota

[Histonet] polymer detection

I would like to get your opinion about rabbit/mouse universal polymer detection systems. Are there any out there that have little or no background when used on canine ,feline, ovine, bovine, porcine, equine or caprine tissues? Right now I use separate polymers for rabbit or mouse antibodies.

[Histonet] Nile Red dye

Is there another name for Nile Red? Could it be a florescent dye? I couldn't find it on the Biological Stain commission or in a reference book from Harleco. I don't know what the stain will be used for. Margaret Perry HT(ASCP) Veterinary & Biomedical Sciences Department North Campus Drive Box

[Histonet] gloves

Where do you buy the Sempermed gloves? Margaret Perry HT(ASCP) Veterinary & Biomedical Sciences Department Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.ut

[Histonet] what is this item called

I found something at tedpella.com. It's a glass bottom dish, catalog number 14026. There are several sizes. It looks similar to the one you posted. Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 _

[Histonet] PVK stain

We are in need of some help trouble shooting this stain. Is it normal for the tissue nuclei to stain red? If not what are we doing wrong? The Chlamydia are staining OK. Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 5700

[Histonet] cytokeratin Lu5

Has anyone used this antibody on dog? If so which company did you use? Would you be willing to share your protocol? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___

[Histonet] picric acid

I am curious how big an explosion there would be from 1% picric acid in acetone if a little dried around the cap. Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___

[Histonet] H&E stainers

We are in the market for a new H&E stainer. We probably won't be doing any special stains with it. Does anyone use the Shur/stain automated slide stainer by TBS? If so what kind of repair record does it have? Are you satisfied with it? Thanks for any info you would like to share. Margaret

[Histonet] Circo antibody

We are running out of our circo antibody from Iowa and did not find it listed in their bank. Are there any recommendations for a different source? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 _

[Histonet] Need help with Laminin

I am trying to work up Laminin in dogs and cats. I have tried the antibody from Sigma L9393 with a variety of retrieval methods both enzymatic and HEIR with no success. I would like to hear from anyone who is testing for laminin. Do I need to use a different antibody? Margaret Perry HT(ASCP)

[Histonet] looking for

Does anyone have an e-mail address for Dick Dapson, Clive Taylor, and William Grizzle? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list

[Histonet] nonstructured proteins

I am posting this again to see if anyone has worked with nonstructured proteins. Do they form the same crosslinks when fixed with formalin? Would antigen retrieval be of any use? Do they react with IHC the same as structured proteins? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedic

[Histonet] RE: klotz solution

We have the same problem. All we do now is make up the solution and leave out the Chloral Hydrate. I haven't talked to the pathologist about it so I don't know if it worked OK. Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings

[Histonet] unstructured proteins

Have any of you worked with the unstructured proteins? Do they form the same crosslinks when fixed with formalin? Would antigen retrieval be of any use? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 __

[Histonet] laminin and collagen4 in cat and dog

We want to use laminin and Collagen IV to sort out the origin of some spindle cell tumors. Specifically, malignant peripheral nerve sheath tumors reportedly show laminin and collagen IV basement membranes vs. Fibrosarcoma which does not. We need antibodies that will work in both cat and dog.

[Histonet] canine teeth

Hi I was given blocks today of a canine tooth that had been decaled for 8 days. We processed it on our regular overnight program. It was too hard to cut and popped out of the paraffin. The biopsy trim sheet did not indicate it was a tooth so I put the pieces in ammonium hydroxide for 10 min.

[Histonet] microwave processors

Has anyone used the LabPulse microwave processor from EBS? We need a new microwave for staining and retrieval and thought maybe we could get one that will do processing also. I've looked at the Shurwave from Fisher, and the Mars from Hacker. Are there any other companies that have small mic

[Histonet] RE: Stupid rabbit antibodies

Have you tried the Dako Rabbit envision? We also tried the AbCam Expose Rabbit specific HRP and used Nova red from Vector or the DAB in the kit depending on which antibody we used. It is excellent , maybe even a little better than Dako's envision. I did have to use blocks such as FC recep

[Histonet] liver endothelial stains

What antibody would you recommend using to label the liver endothelial cells? Is factor 8 our best marker? Also do you know of a special stain other than H&E that would demonstrate these cells? Thank you. Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota

[Histonet] special stains QC

Our lab would like to know how you handle the quality issue of verifying that the control material you use for special stains is what it's supposed to be. For example: how do you confirm that bile in a piece of liver used as a Halls control is actually bile? We use controls for bacteria or virus

[Histonet] FW: WNV

From: Perry, Margaret Sent: Monday, September 27, 2010 11:41 AM To: 'tgo...@mt.gov' Subject: WNV We have a problem with the equine tissue as do other labs. A lot of samples can be done and only one will come up positive. We have good luck with avian tissues. We use DAKO envi

[Histonet] drying ovens

Has anyone used the TDO66 Medite drying oven? Do you like it? Is the company good to work with? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet ma

[Histonet] coverslip removal

I am using aqueous mounting media, faramount, for the first time. The slides I coverslipped several days ago look dry. Will soaking in water remove the coverslip? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605

[Histonet] modified Mayers Hematoxylin

We are looking for a procedure for modified mayer's hematoxylin. The pathologists don't like the Gills. We need something that is close to Mayer's in staining. We were told to find something without the chloral hydrate. We have found a modified Hematoxylin from Thermo that the doc's like bu

[Histonet] muscle stain

I am posting this for a friend. . They would like to stain muscle. The eosin and hematoxylin stain gives us too much information since it stains the nucleus separately from the cytoplasm and confuses the image analysis software. What we need is the measurement of the cell diameter only, a simpl

[Histonet] metal base molds

The following link has larger molds, maybe one of them will work. http://www.brainresearchlab.com/productinform.html Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 Message: 20 Date: Thu, 12 Aug 2010 11

[Histonet] RE: Histonet Digest, Vol 81, Issue 1

What antibody are you trying to use? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Sunday, August 01, 2010 12:01 PM To: histonet@lists.utsouthwestern.edu S

[Histonet] EXPOSE

Has anyone tried the new polyvalent polymer detection "EXPOSE" from AbCam? What did you think of it? Margaret SDSU ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] slim jims

Do you put the slim jims in formalin and then process them or just put them in the processor? Margaret ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] xylene substitutes

We use formula 83 for everything and do not have a problem with a film. We have found that we must use isopropal alcohol instead of reagent alcohol in the final dehydration steps. Margaret Perry SDSU ___ Histonet mailing list Histonet@lists.utsouthwe

[Histonet] paraformaldayhde

In the Brown and Hopps gram stain can I substitute 37 g in 100 ml of powdered paraformaldyhyde for the 37% formalin or do I need to buffer it? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/h

[Histonet] Gram stain

Now that I'm done with my rant I have a real question. We are trying to do a gram stain on fish and the safranine O is staining everything red. What other stain would you use? I usually have time to look at the books but unfortunately it's almost quiting time and the slides need to be done by

[Histonet] new DAB

When did DAKO retire their DAB and only stock the DAB plus? Since it's Friday I can rant. I am so disgusted that no one was notified of the change. With QC I'm going to have to revalidate or prove that the New DAB works as well as or better than the old. Of course I had to be a klutz and spi

[Histonet] GMS stain

We sometimes have problems with the stain if we use positive slides. Margaret ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] elisa's

Is there a list serve for elisa's? Margaret Perry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] alcian blue van gieson stain

We want to try the double stain alcian blue Van gieson. Do you stain for the alcian blue first and then the Van gieson? We do both stains but I am unsure of how to put them together. Margaret Perry South Dakota State University ___ Histonet mailing l

[Histonet] looking for mouse antibodies

Are there good mouse antibodies that will work on dog and cat for the following: Factor 8 GFAP Mycobacterium sp S100a ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Klotz fixative

Do you know of anyone that sells Klotz fixative? We use it in a pre vet class but would like to eliminate the choloralhydrate since it is such a hassel due to it's being a controlled substance. Is there a substitute fixative that would retain the properties of Klotz? _

[Histonet] need help with stain

A researcher wants to measure the length of the intestinal crypts and asked for a suggestion on what type of stain to use. I am thinking of using a phloxine/tartrazine stain and do a double stain with a GMS to show the basement membrane. What is your opinion on this? Do you have other suggest

[Histonet] alcian blue stain

We are really getting new research procedures lately. As with almost all published papers the methods are a quick overview without details. We have been asked to do a combination stain of H&E with alcian blue. Would you recommend doing the alcian blue 8GX pH 2.5 first, doing it after the hem

[Histonet] fish scales

We are trying to cut fish scales that have been decalcified. They are chunking out when we try to cut them. I think we need to soften the keratin and I looked in the archives for the right dilution of ammonium hydroxide to use. One post said 5% the other said straight. What dilution do you r

[Histonet] RE: Mycoplasma special stain

Hi JoAnne, Our pathologist requested we try a Faulkner stain for Mycoplasma. I found two methods. The Faulkner/Lillie and the Faulkner/Kerr variations of the Warthin Starry. One of these is a lower acid. The pathologist is looking up the reference and will call the people who wrote a paper o

[Histonet] RE: Antigen retrieval question

If we need to hold retrieved slides for awhile we hold them in water up to 4 hours. Holding in water overnight leads to no signal. We have also tried holding the slides in our Tris buffer and have found decreased or no staining. Margaret Perry ___ His

[Histonet] Warthin Faulkner method for spirochaetes

We are trying to work up this stain per pathologists special request. We usually do the Warthin-Starry method not this modification. I have the published article from Stain Technology Vol. 20 No. 3 July 1945. The protocol is a little vague and I have some questions? Step one brings the slide

[Histonet] tris buffer

I am trying a protocol that uses 0.1M Tris/0.15M sodium chloride pH7.5, which is what I normally use. At another step in the protocol it says to wash with 0.05M Tris buffer pH 7.5. What do I use to make the 0.05M Tris buffer? Is it a combination of Tris HCl and Tris base without sodium chlorid

[Histonet] Tissue Embedder

We are in the market for a new embedder. What do you suggest? I tried searching the archives but only came up with older comments. Vendors please feel free to contact frank@sdstate.edu. We may have to go with a used instrument so would like comments on good

[Histonet] West Nile

Has anyone found a good antibody for west Nile FFPE that will work on horses? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Start Up Lab

I would add an eye wash station and a chemical spill kit to the list. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 ___

[Histonet] Thermo Fishe maintenance contracts

Do any of you use Thermo-Fisher for your maintenance contract consolidation? What kind of service do you receive and are the people who do the service competent? Have you had any problems and were they resolved quickly and efficiently? Was it easy to drop and add items from the contract? Ple

[Histonet] time in formula 83

Our processor has gone down but we can process up to the paraffin step. My question is how long can we keep the tissues in the formula 83 without any visible problems? We would like to start processing tonight and possibly infiltrate by hand tomorrow. Not optimal but it does work. Your comme

[Histonet] Mycobacterium bovis

I have been looking for a replacement for my antibody from DAKO. It has been discontinued. I've tried the internet and several antibody search engines with no luck. Can anyone point me in the right direction. I am doing IHC paraffin FFPE Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Sc

[Histonet] ground meat samples

We will be doing IHC on ground pork and would like tips on how to process and embed the samples. Can you leave the processed sample in a paper biopsy bag and embed both the meat and the paper? Will cutting the paper make the blade dull to quickly? Is there anything else we can use to hold the

[Histonet] protease XIV

I'm trying to find a source for protease XIV other than Sigma. Any suggestions? Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007

[Histonet] Looking for Patsy Ruegg's e-mail address

Patsy I have a couple of questions for you but have the wrong address. Margaret Perry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE:DAKO

I don't understand why your prices are so different from mine. I just got a new quote from my Rep and my price increases were very reasonable. There seems to be a large discrepancy in prices across the USA. I would call the office at DAKO and complain or like you said change vendors. I would

[Histonet] problem eyes

Please help! We have been trying to cut whole eyes from a pig. They need to be nice enough for a publication. We are having the devil of a time because no matter what we do there are wrinkles. If we turn the waterbath up to stretch things out the retina detaches. Do any of you have suggesti

[Histonet] brain sections falling off slides during IHC

A few years ago we also had this problem. The brains were terrible. We found that changing our processing chemicals, especially the wax, more frequently got rid of most of our problems. We use Fisher plus slides. There is a difference in plus slides. I recommend you contact several distribut

[Histonet] Coxiella

Gayle, I was very interested in the comment you made about Coxiella not being killed with formalin fixation. I have found references for 5% formalin and formaldehyde gas but did not find references addressing 10% formalin. Will you provide more references that I can show my pathologist? Thanks

[Histonet] lids for stainer containers

We are looking for a source that sells a lid that looks like wax covered cardboard. We use them on top of the Shandon slide containers holding xylene or formula 83 while waiting to coverslip. They are about 5x7 and very thin. They have been here for years so I have no idea where they came fro

[Histonet] HIER

We have been using the microwave HIER and have had good results, however when our microwave bits the dust I would like to have a pressure cooker method in place. I feel the pressure cooker is more consistent for all the slides. We are a veterinary diagnostic lab and I would like to have some i

[Histonet] methylene blue

Help! We have been asked to do a methylene blue stain for Myxosporidia and don't have a procedure. Of course the pathologist wants it quickly. I can't get into the archives. It must be down right now. Can someone help us? Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science