RE: [Histonet] Number of blocks

Rene probably can't since Histonet does not allow attachments (definitely a good thing). Rene might have problem with journal copyright issues as well. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax

RE: [Histonet] Number of blocks

Brilliant!!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145

[Histonet] RE: help with dead fungi

I would use a Grocott's Methenamine Silver (uses chromic acid) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street

[Histonet] Please copy to the list when replying to posts

We seem to be missing discussions on some emails. Please share your suggestions with us all by replying all Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead

RE: [Histonet] Polyoma for renal transplantation

kidneys, BK virus is found in areas of interstitial nephritis. REFERENCES: Nickeleit et al (1999) Polyomavirus Infection of Renal Allografts J Am Soc Nephrol 10: 1080-1089. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612

[Histonet] RE: air drying special stain slides rather than

. Whoops, I forgot about the Oil Red O stains for fats, Oh well I did say nearly all!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road

RE: [Histonet] RE: air drying special stain slides rather than

Yep Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA

RE: [Histonet] Re: Teabags

Agree totally Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145

RE: [Histonet] Teabags

Cheaper! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145

[Histonet] RE: counterstain for Alcian Blue (ph2.5)

or even just a light eosin counterstain. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001

RE: [Histonet] Secondary antibody causing nuclear staining?

(Mount Cooper 2001). Mount SL Cooper K (2001) Beware of biotin: a source of false-positive immunohistochemistry Current Diagnostic Pathology 7:161-167. Nakatani et al (1994) Am J Surg Pathol 18(6):637-642. Sickel di Sant'Agnese (1994) Arch Pathol Lab Med 118:831-833 Regards Tony Henwood JP

RE: [Histonet] Secondary antibody causing nuclear staining?

I should have added that this was from the workshop notes on a Hypotheticals Workshop I ran last year at our Australian National Meeting. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318

RE: [Histonet] Re: Methanol in H2O2 explanation

So, the thinking at the time was to kill the endogenous peroxidase twice! No one can say that us oldies weren't thorough! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's

[Histonet] RE: Another Microscope Question

image patterns. A checkpoint for quality slides. American Journal of Dermatopathology. 4(2):143-8. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr

[Histonet] RE: Re: Methanol in H2O2 explanation

their thing efficiently. I was lead to believe that as H2O2 was catalysed by endogenous peroxidase, the reactive oxygen reacted with the methanol to then degrade the enzyme, but I need to look closer at this chemistry. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA

[Histonet] RE: Monoclonal antibodies from different companies

Hi Teri If you can get a monoclonal antibody from different sources, the same clone, the same Ig concentration, IT IS THE SAME. There is no any benefit in buying the antibody from Company B to try, since it is a duplication of effort and cost Regards Tony Henwood JP, MSc, BAppSc

[Histonet] RE: Monoclonal antibodies from different companies

Gee I do live in an ideal world, looking through rose coloured glasses - definitely affects my interpretation of an HE stain! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's

RE: [Histonet] Fwd: Plants

Well, Why are we not surprised that the higher-ups have the authority but little knowledge? At least Stephanie's Bosses decisions cannot be taken as gospel for the rest of us! (ie we can ignore them) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory

RE: [Histonet] Fwd: Plants

If referring to a Histopathology lab, what a load of codswallop. Don't we love supposed Professionals who make decisions based on wrong information. I would suggest that the Infectious Control person do some further study on other areas of pathology. Regards Tony Henwood JP, MSc, BAppSc

RE: [Histonet] Fwd: Plants

I would suggest that it is the potting mix that is the culprit not the plant. But then remember Histo laboratories are not ICUs nor are they Microbiology laboratories. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612

RE: [Histonet] Methyl green going away...

You could try dehydrating in acetone (very quick). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead

RE: [Histonet] In House Labs in WSJ

I find it interesting (and slightly amusing) that a professor of pediatrics is chairwoman of the task force on PSA testing. After my early publications on PSA IPXs, I thought that I was over that now I am in a Children's Hospital. Now I am not so sure! Regards Tony Henwood JP, MSc, BAppSc

RE: [Histonet] Need the name of a piece of equipment

Thermo's Para-Trimer is probably what you are after. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead

[Histonet] RE: Phosphorylase method

muscle fibres and background yellow-brown Smooth muscle in artery walls purple References Martinuzzi et al (1999) McArdle's Disease - The Unsolved Mystery of the Reappearing Enzyme American Journal of Pathology.;154:1893-1897 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT

[Histonet] RE: An immunologist...

Well, don't leave me hanging - what happened. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked

RE: [Histonet] Re: Redneck Lent

Roman or Anglican or Episcopalian? Well I'm Australian!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street

RE: [Histonet] FW: Redneck Lent

Yeah, But it is still funny (from a laugh-at-myself catholic) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street

[Histonet] RE: MSDS regarding waste alcohol, xylene, etc

) - that could be difficult Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW

[Histonet] RE: When to toss reagents?

From the seventies is not necessary a bad thing. Most you will find to be quite useable. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury

[Histonet] RE: MSDS regarding waste alcohol, xylene, etc

take precedence. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145

[Histonet] RE: Fixation for breasts

Try Microwaving blocks in 10% NBF or alcoholic formalin for 2 hours at 45oC. Works a treat with placentas Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital

RE: [Histonet] PAS Diastase

My two cents: I agree as well Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001

Recall: [Histonet] PAS Diastase

Tony Henwood (SCHN) would like to recall the message, [Histonet] PAS Diastase. * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom

[Histonet] RE: Re: PAS Diastase

No, If you need to demonstrate that PAS positive material is glycogen and not some other carbohydrate containing material you must do a PAS on the test case as well. (see my earlier Post) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior

[Histonet] Wet Workshops NSW Australia

Manual Immunohistochemistry Any other ideas? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) and Linda Prasad MSc, BSc Histopathology Department Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street

RE: [Histonet] Re: Gram stain

I agree with Bob wholeheartedly as well as agree 100% and I am unanimous in that! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road

[Histonet] RE: Gram Stain

the gram negative bugs. A simple red counterstain is to stain sections with 1% aqueous neutral red for 3 min after the crystal violet-Iodine steps Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318

RE: [Histonet] Shiny side of a paraffin section

Who wants to look at Cell bottoms all day!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked

RE: [Histonet] Shiny side of a paraffin section

staff call it Tony's Migraine Quiz - one 'properly' collected section and the second a rebelliously collected shiny-side up section. 4. Microscopically, when comparing special stains with a HE, it can drive you to distraction when microscopic features do not line up. Regards Tony Henwood

RE: [Histonet] Does xylene cause skin cancer?

(http://www.bioc.cam.ac.uk/safety/glove_guide.pdf) (2) Ansell Chemical Resistance Guide (http://www.ansellpro.com/download/Ansell_7thEditionChemicalResistanceGuide.pdf) http://www.aps.anl.gov/Safety_and_Training/User_Safety/gloveselection.html Regards Tony Henwood JP, MSc, BAppSc

RE: [Histonet] Does xylene cause skin cancer?

Wow, That's interesting; KIMBERLY-CLARK Nitrile Gloves are not resistant to xylene, whereas Ansell's Nitrile gloves are (see http://www.ansellpro.com/download/Ansell_7thEditionChemicalResistanceGuide.pdf) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory

RE: [Histonet] (no subject)

: 803-805 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA

[Histonet] RE: Processing adipose tissue

wax impregnation is at 60oC or more. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001

RE: [Histonet] Interview Questions

Joe, I would never wear a denim miniskirt! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked

RE: [Histonet] Oil red O versus Sudan 4

Candice, Remember freshly prepared Oil Red O Stock usually does not work. Prepare your saturated Oil Red O Stock and leave it for a week or more before using it to prepare a working solution. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager

RE: [Histonet] Question regarding staining of ligament tissue using HE. The nuclei in our ligament tissue is not staining consistently.

eosin staining as well - look at the contrast between collagen, smooth muscle and nerves). After xylene and alcohol, check the water rinsed slide. The tissue should not be hydrophobic. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior

RE: [Histonet] Pearls Prussian blue with green counterstain

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original

RE: [Histonet] Music in the Laboratory

to a song that seemed to contain naughty words. Fortunately I had no idea what the words to the song were (I needed sub-titles) - and that seems to be the case with most of them now. My hearin is probably shot from too much Deep Purple, Black Sabbath and ACDC anyway!! Regards Tony Henwood JP, MSc

RE: [Histonet] Re: Formalin Neutralizing

Thanks Rob for reminding me. It was John A. Kiernan who originally posted the message that I refer to below on Histonet back in 2002. As everyone will agree John is a great teacher and one of our National Treasures in Histotechnology. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys

[Histonet] RE: Elastichrome paper, and troubleshooting

How old is the alcoholic Hx you use to prepare the Verhoeffs. I have found that matured 10% ethanoic Hx is more resistant to differentiation than freshly prepared Hx. Might be of use Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior

RE: [Histonet] Has anyone else noticed

Remember we must validate, repeat, validate, repeate, vaallidatee, reeepeeaaat, valid. OMG its blurry again Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's

RE: [Histonet] Has anyone else noticed

I keep trying to verify this scientific discovery but everything is blurry by the time I get to the bottom of the bottle, Oh sorry I need to look in the glass? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306

RE: [Histonet] bacteria staining

Mesru, Use 1% aqueous Giemsa, stain dehydrated sections 5-10 minutes, dehydrate rapidly (either acetone, air dry or rapid alcohol dehydation), clear and mount Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306

[Histonet] RE: help

and technical review Arch Histol Cytol 70(1): 1-19. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag

RE: [Histonet] counterstain for fast red

Try 1-2% Ethyl green in acetate buffer (pH4) - will give you green nuclei (of course) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury

RE: [Histonet] My bit of trivia for today - True Blue

the load? Will you tie it up with wire, Just to keep the show on the road? Hey True Blue, Hey True Blue, now be Fair Dinkum Is your heart still there? If they sell us out like sponge cake Do you really care? Hey True Blue. If you would like further translation please ask!! Regards Tony Henwood

[Histonet] RE: processing program for biopsies

on the machine would be great)). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001

RE: [Histonet] microwave processing

I believe that the higher temperature is needed to evaporate off the isopropanol. Just make sure that your tissue is well fixed. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318

RE: [Histonet] (no subject)

Miha, Are your muscles arising in saline? Receive them dry or in cell culture media (eg Hanks). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr

RE: [Histonet] EDTA

José, I have inserted my ideas below within your email. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street

RE: [Histonet] ovarian cryosections

Carmen, If the tissues have been fixed in formalin, then this would not be a problem, but if not, why not freeze the tissues in OCT (or similar) and store it at -70oC before cryotomy, rather than placing it in a sucrose solution? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC

RE: [Histonet] will reembed frozen tissue in OCT affect the histology

and enzymatic activity (Horkayne-Szakaly et al (2009) Nonfrozen Transport Medium Preserves and Restores Skeletal Muscle Enzymatic Activity and Morphology J Histotechnol 32(2):49-53) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel

[Histonet] RE: RPM on Cytocentrifuge for CSF

500rpm for 5 minutes using the Shandon Cytospin 3 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead

RE: [Histonet] 0.5% acid alcohol

rather than 70%) What is it to be used for? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag

[Histonet] RE: Brigati Stable DAB

to 50ml Tris Buffer-Wash 3. Add 50μl Hydrogen Peroxide 4. Incubate slides for 5-7min until sections appear brown Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's

[Histonet] RE: PAS

Mirna, Lee Luna (Histologic 6(1):77, 1976) suggests that the best control for PAS staining, are the walls of blood vessels. They will show a bright red reaction only if the technique is working well. Glycogen may stain well even with sub-optimal reagents. Regards Tony Henwood JP, MSc, BAppSc

RE: [Histonet] RE: Retirement

(though I would probably be the last to know). But more importantly I am too young to be married to a Grandmother. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital

[Histonet] RE: Decal Solutions

Vicki, We fix our trephines in 10% formal alcohol overnight and decal for 6 hours in formic acid (4% Formaldehyde, 33% Formic acid, 0.85% Sodium Chloride). Excellent morphology and good antigen preservation. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA

RE: [Histonet] Submission of tonsils to pathology

period - 1 month from date of issue of specimen report (Australian NPAAC Standard 2009). In our department we keep them for 3 months. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318

RE: [Histonet] Submission of tonsils to Pathology - #2

in workload. The small pots that the tonsils arrive in did not affect our storage capacity. So in our situation it was not a big deal to accept these specimens. But this might not be feasible for all labs. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory

RE: [Histonet] Lipofuscin and counterstaining DAb IHC

Csaba, I can't answer the fluorescent question but for light microscopic IHC I would stain for the NeuN using peroxidase-DAB followed by a PAS for lipofuscin followed by Mayer's or Harris's haematoxylin. Result is Brown, pink, blue Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT

RE: [Histonet] Alternatives to Cytospin?

available, place a drop of the BALF on the slide and either air dry as you suggested (if cell count is low) or treat it like blood (if it looks quite opaque - lots of cells/ other junk). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior

RE: [Histonet] Help with Masson's Trichrome - not working on cryo sections

Jennifer, Looking at the images, is it possible that the slides were air-dried for too long prior to staining? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital

[Histonet] RE: IHC pos. neg. control question

my negative control. I already know these do not occur on my positive control tissue. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road

RE: [Histonet] IHC pos. neg. control question

either would fulfill the purpose of detecting non-specific positive staining - NO Not in the patients sample unless it is included (which for diagnostic uses is the most important). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior

RE: [Histonet] preference: fat stains

Definitely go with Oil Red O rather than Osmium. Osmium tetroxide reacts with unsaturated lipids whereas ORO colours neutral lipids. You can do an ORO on formalin fixed tissue (frozen sections). You could also look at the Sudan Black techniques. Regards Tony Henwood JP, MSc, BAppSc

[Histonet] RE: Biopsy Run

Increase the fixation time. If they are small biopsies (otherwise why would you be putting them in mesh cassettes), you could easily reduce the processing time to 30 minutes (eg 4 alcohols, 2-3 xylenes, 2-3 waxes) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA

RE: [Histonet] Re: processing times for skin specimens

This is important. Ensure that your fixation time is as long as possible. Short processing will give poor results if fixation is shortened Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318

[Histonet] RE: endogenous peroxidases

)). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA

[Histonet] RE: IHC on frozen sections

frozen sections. Any reason why you have chosen the frozen section of formalin fixed tissue? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury

RE: [Histonet] un-nucleated squamous epithelial cells

So does that mean I will have to wear makeup? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked

RE: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case?

. Formalin dilutes as it penetrates tissue. 3. Formalin substitutes may not be germicidal. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr

RE: [Histonet] Counterstain for dual chromogenic

staining Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA

RE: [Histonet] Von kossa stain

thiosulphate and cannot be demonstrated with von Kossa's technique (Meloan Puchtler 1985 J Histotechnol 8(1):11-13.). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital

[Histonet] RE: Biopsy program for Sakura VIP 5/6 Processor

Increase the fixation time and make sure the air is out of the sponges (will stop formalin from getting to the tissue- a quick dunk of the cassette (with sponge and tissue) in alcohol and back into formalin will do the trick). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC

RE: [Histonet] RE: Biopsy program for Sakura VIP 5/6 Processor

I agree Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA

[Histonet] RE: Verhoeff/Masson's Stain

be easily seen. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA

RE: [Histonet] Verhoeff/Masson's Stain

See there are always differing experiences (see my earlier post) How are you Bryan, well I hope? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr

RE: [Histonet] Control Slides

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original

[Histonet] RE: cell blocks

Sarah, It is important that the agar cell block is allowed to fix in formalin (10%NBF) before wrapping in paper, at least 2 hours. It will hold its shape better. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845

[Histonet] RE: Preferred green counterstain for GMS?

Ally, I would use Fast Green FCF. It does not fade as much as Light Green. Use a 1% in 2% Acetic acid. Tartrazine will give you a yellow colour. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845

[Histonet] RE: alcian blue-PAS control

? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA

[Histonet] RE: saliva for glycogen hydrolysis

into a 50ml specimen container and add PBS buffer, equal volumes, mix and pipette on to the slides and incubate for 10-15 min at room temp. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318

RE: [Histonet] Amylase Digestion for glycogen

. Place in Schiff's reagent 10 minutes. 8. Rinse slides in distilled water and then wash slides in tap water 3 minutes. 9. Counterstain slides with haematoxylin, differentiate and blue. 10. Dehydrate, clear and mount. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC

RE: [Histonet] CSF for cytology

even concentrate the cells using centrifugation prior to cytocentrifugation. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road

[Histonet] RE: Bacterial contamination

the sections for Gram, ZN and Fungi staining first thing in the morning to alleviate the problem. Only a thought! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead

[Histonet] RE: Parathyroid staining

Dorothy, A 0.5% aqueous Toluidine blue will suffice. Stain either air-dried or alcohol fixed frozen sections for 1-2 minutes, rinse in water, dehydrate quickly, clear and mount. That's it! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel

RE: [Histonet] need ur help

in fixative in a warm oven (37-45oC) before loading onto the processor. Are they being allowed to dry before being placed in fixative? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital

RE: [Histonet] need ur help

Kim, Did you send this to Histonet Wassan? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead

RE: [Histonet] Partially muddy slides

Michelle, It might possible be water contamination in the xylene. Try increasing alcohol dehydration times. Also re-cyled alcohol is probably around 96% pure. It seems to be not possible to obtain absolute ethanol by re-cycling, or so I am lead to believe. Regards Tony Henwood JP, MSc, BAppSc

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