[Histonet] Zeus (Michels) fixative and buffer wash

We purchased from Poly Scientific Corp, both the transport media (fixative) and the buffer wash. It comes in several sizes. Another supplier is Newcomer Supply. Gayle Callis GCallis Histology Service LLC Bozeman MT ___ Histonet mailing

[Histonet] Attention Gudrun Lang Re EDTA method for bone marrow biopsies

Dear Gudrun, EDTA will always be slower than a buffered formic acid and there are caveats about using it for some enzyme methods for bone. This EDTA method which is better than most but is avoid pH that is too low. This EDTA method is made with tetra-sodium EDTA (Fisher BP 121-500 which ha

Re: [Histonet] Decal following ISH?

the researcher do fixation, then EDTA decalcification before ISH particularly if the signal is ruined on snails with shells. I bet it would make his results better too - no shell to deter penetration of reagents. Good luck and let us know if you have success. Happy Holidays!!! Gayle Callis

[Histonet] Kawamoto protocol online from Rowe lab

invaluable, with Protocol and Materials. You can also access a pdf of protocol. Video access is on home page of this website. You will see cryomicrotomy, snap freezing and other techniques to help you. Good Luck Gayle Callis HTL/HT/MT(ASCP

[Histonet] Sandersons Rapid Bone stain chemistry and history

entually the stain does not work very well and you need to start with new stock. We preferred to use a MacNeals tetrachrome combined with toluidine blue per Sterchi method for more brilliant staining of osteoid other bone components including cartilage. Take care Gayle Callis HT

[Histonet] Attention Daniel Blackburn about plastics embedding

. Take care Gayle Callis HTL, HT, MT(ASCP) GCallis Histology Service LLC Bozeman MT/USA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] glycol methylacrylate GMA enzyme and IHC

e with IHC using PMMA.Using this plastic also requires stringent safety precautions for handling the chemicals. Sorry I leaned towards a negative view of GMA. Take care Gayle Callis HTL/HT/MT(ASCP) GCallis Histology Service, LL

[Histonet] Re #2: μm H&E staining, WHOOPS!!

Sorry about hitting send too soon. Repeat of things to try: a. Do not use regressive hematoxylin and eosin where hematoxylin can be overly differentiated i.e. removed from too thin sections. Use progressive hematoxylin i.e. Gill II or Gill III type formulation. DO NOT use acid a

Re: [Histonet] 2 um sections H&E staining

You wrote: When I cut at 2μm my H&Es and special stains look pale. How can I get my stains to pop or am I stuck with pale looking stains when sectioning that thin? I run manual specials and a manual regressive H&E. For H&E I've tried increasing my time in hematoxylin (beyond the manufacturer r

Re: [Histonet] Bouins for testicular biopsies

Richard, You wrote: What are people fixing testicular biopsies in to evaluate infertility? In the past, I believe fixatives such as Zenker's and Bouin's were used for this purpose since they enhance nuclear detail. Obviously, those fixatives can no longer be used. Thank you.

[Histonet] NSH Journal of Histotechnology reminder on call of manuscripts for troubleshooting stains due July 1st

Dear Histonetters, This is a reminder for people to submit a manuscript on troubleshooting H&E and special stains on paraffin embedded tissue sections. You do not have to be a member of NSH to submit manuscripts to the Journal of Histotechnology. JOH wants to know how you solve staining p

Re: [Histonet] Reprocessing

A quick technique, published in Histologic Vol XXXVI, No. 1, May 2003 http://www.sakura-americas.com/Histologic/Histo-Tips/1008.htmlby Michael Johnson, A technic for correcting poorly processed paraffin blocks. Melt the paraffin, blot to remove excess melted paraffin, place in cassett

[Histonet] Journal of Histotechnology special issue call for manuscripts

Dear Histonetters, The Journal of Histotechnology is calling for manuscripts to be published in December, 2016 . The manuscript submission deadline is no later than July 1st to allow for a review process. Any late submissions would be published in a future issue.This is a special issue on

[Histonet] glycine method to remove aldehyde induced autofluorescence

ne is cheap and goes into solution readily. FYI, lysine has also been used to get rid of free aldehydes (Elias J. Immunohistopathology book) Good luck Gayle Callis HTL/HT/MT(ASCP) Bozeman MT ___ Histonet mailin

Re: [Histonet] auto-fluorescence

but these photograph beautifully. OR if you have a spectral imaging or confocal capabilities, you can rule out autofluorescence without having to treat the tissue section. If you have difficulty access this pdf, I will send via private email. Gayle Callis HTL/HT/MT(ASCP) Boze

Re: [Histonet] formalin fixed tonsil frozen sections

Dear Erin You wrote: Good morning! My pathologists would like us to cut formalin fixed (not yet processed) tonsil tissue on a cryostat for DIF staining. Has anyone done this? I did a quick search that seemed to indicate that it was possible but that the architecture of the cells would

Re: [Histonet] Glass knife holder

blade one at a time with a fine forceps, and then float it on RT distilled water. A glass staining dish has always worked for this. Good luck and hope you have some success. Gayle Callis HTL/HT/MT(ASCP) ___ Histonet mailing lis

Re: [Histonet] Hematoxylin Precipitate and filtering Gill formulations

Yes, I have used Gill 1, 2 and 3 even in the early days of buying these formulations from a vendor, and always filtered them before using. Old school habits never changed.. Gayle Callis -Original Message- From: Manfre, Philip via Histonet [mailto:histonet

Re: [Histonet] Hematoxylin Precipitate

Sandy, After years of using Richard Allan's hematoxylin 2 with great success, if we didn't filter daily before use, we had stain precipitate on sections. Some of this comes from the hematoxylin continuing to oxidize in open air, bacteria and other "crud". Tim is absolutely correct ignoring ma

Re: [Histonet] Long reply on problems with hydrogen peroxide blocking

ng reagent background test. I will be happy to provide the simple background test too. Take care Gayle Callis HTL/HT/MT(ASCP) Amos and Patrick Wrote: Hi, Peroxidase can really be a pain. If you look in the archives though (or ask her really nice) Gayle Callis submitte

[Histonet] Re. Decalcification with formic acid sodium

Thanks everyone Gayle Callis You Wrote: There was a paper <http://www.genedetect.com/Merchant2/ExampleRefs/Decalcifying_protocols.pdf> http://www.genedetect.com/Merchant2/ExampleRefs/Decalcifying_protocols.pdf Talking about formic acid (Morse solution) c

[Histonet] Re. Decalcification with formic acid sodium citrate

Merissa and Tim, This formic acid decalcifying solution is basically the classic Evans and Krajian fluid (Sheehan and Hrapchak, Theory and Practice of Histotechnology, 2nd edition, P.92). Shandon has added other ingredients for some reason, and has kept those concentrations proprietary.

Re: [Histonet] mouse leg decalcification

Good luck Gayle Callis HTL/HT/MT(ASCP) You wrote: We do mouse legs routinely, either with Cal Ex II or EDTA. We look at the bone and the tissue around, so we have to have good fixation, decal., cutting and staining for both. Cal Ex II (formic acid) is a gentler decal a

Re: [Histonet] Trichrome troubleshooting

with acetic acid and will automatically do this, even on Weigert's Iron hematoxylin. We also examined our sections microscopically during staining to make sure the check the depth of red staining reagents on smooth muscle is correct - once again, Liz is correct about this fact. Ga

Re: [Histonet] Trichrome troubleshooting

Suzanne, I don't understand what you wrote here " We are using Leica's kit with the Weigerts iron with Gills". In a long histo career, I never used Gills half oxidized hematoxylin for a trichrome stain nor heard of the combined staining you mentioned. Could you explain what you are doing

Re: [Histonet] Xylene substitutes in staining line

You wrote: Hello Histo Land, Does anyone use xylene substitutes in their stain line only? I am especially interested in d-limonene options. Thanks in advance! * We used

Re: [Histonet] Cryojane bone frozen sections and immunofluorescence

Everyone wrote: Hi Ana, The OCT on the coated slides will not disappear like it does on regular glass slides. It appears to polymerize along with the coating, but it doesn't seem to interfere on the tissue section with the staining. According to Gayle Callis' recommendation,

Re: [Histonet] toluidine blue for cartilage with controls and mast cell staining

You wrote: We use a canine mast cell tumor as positive control - veterinary lab naturally. Probably looking for mast cells in the core. Tresa -Original Message- From: Bernice Frederick [mailto:b-frederick at northwestern.edu

[Histonet] RE..... murine CD4, CD8 and CD68 for FFPE tissue

You wrote: Is anyone of you familiar with the possibility of applying CD4, CD8 and CD68 antibodies on formalin fixed paraffin embedded liver of mice? Thanks in advance. Joost Bruijntjes ** Thankfully and at long last after years of frustration by m

[Histonet] Staining net dishes for brain sections

lift the sections in some way. Gayle Callis HTL/HT/MT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Can't log into Histonet to do anything

il address. Stymied and dead in the water. I would love to unsubscribe, but not sure anyone gets this message. Gayle Callis ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Passing onto PAS discussion RE: GMS question

difficult. Thanks for the supportive comment on GMS. Your comments on Histoplasma sp. staining were interesting as not many of us have the opportunity to work with ancient tissues. Gayle Callis -Original Message- From: Bob Richmond [mailto:rsrichm...@gmail.com] Sent: Saturday, May

Re: [Histonet] GMS Question

ain either mature or immature fungal and/or both cell wall structure. Gayle Callis HTL/HT/MT (ASCP) -Original Message- From: Goins, Tresa [mailto:tgo...@mt.gov] Sent: Friday, May 01, 2015 11:29 AM To: Paula Lucas; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] GMS Question

[Histonet] long answer RE: GMS Question

Hi Paula, The same site of infection for your control and patient tissues does not mean the fungus species was the same. Now for addtional commentary. The classic GMS uses chromic acid as the oxidizer, stronger than periodic acid. This is something to beware of since you had a false negative

[Histonet] RE... Frozen section fixation problems

chase away these annoying "gremlins". Gayle M. Callis HTL/HT/MT(ASCP) Patrick, We do a lot of frozen section IHC work. Years ago Gayle Callis turned me on to fixing in cold acetone:ethanol (3:1) . We keep it at -20C and I fix for 10 min. on the bench then wa

[Histonet] Re: Pam Marcum colleague losing bone sections from slides

IF all else fails, try Sterchi tape transfer method with packaging tape. I have the method with photos and publication, and will send privately. Good luck Gayle Callis HTL/HT/MT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.e

[Histonet] Re: Laczko/Levai stain protocol

Methods: Eight beagle dogs received implants randomly installed into . Levai. Laczko staining. Original magnification x 2.5. Fig. 9. Most apical . Good luck Gayle Callis HTL/HT/MT(ASCP) ___ Histonet mailing list Histonet@lists.utsou

[Histonet] Re: TRAP staining on formic acid decalcified bone reference

The reference within a reference from Ray is A Chimeric Form of Osteoprotegerin Inhibits Hypercalcemia and Bone Resorption Induced by IL-1β, TNF-α, PTH, PTHrP, and 1,25(OH)2D3 . Sean Morony et al . J Bone Mineral Res V 14, pp 1478-1485. However, the formic acid decalcification method is no

[Histonet] Staining dishes and slide racks for sale

Dear Histonetters, Janet Maass has these new and used staining dishes, covers and slide racks for sale. I will be happy to forward your email message to her since Histonet messes with email addresses. 22 glass staining dishes with covers (for 50 slides) 4 extra covers available

RE: [Histonet] Acetone fixing and tissue damage

anks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Thursday, March 12, 2015 12:02 PM To: histonet@lists.utsouthw

[Histonet] RE: Masson Trichrome stain

ut a good start. Whew, a long reply but hope helps... Gayle Callis HTL/HT/MT(ASCP) Written is: Justine, I do not have any metal forceps in the special stains area, due to the reaction that they can cause when staining with silver. As a rule of thumb, it is just easie

[Histonet] Acetone fixing and tissue damage

for 10 minutes 5. Air dry to evaporate acetone 6. Proceed to immunostaining Gayle Callis HTL/HT/MT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Re: Bird head stored in 70% alcohol and possible decalcification

You wrote: I have an adult bird skull that fixed with formalin and then has been stored in 70% ethanol. I have seen the post that the sample stored in 70% ethanol can be walking back through to series of ethanol to water and can be decalcified if it needs to be. I am wondering if anybo

RE: [Histonet] Re: Embedding

rkets, discount stores or a recycled frozen food pan without separations. These pans come in various sizes and depths. The joy is toss pans when full of paraffin. Gayle Callis HTL/HT/MT(ASCP) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet

[Histonet] Re: Embedding

After years of never winning the battle of paraffin on cassette edges after embedding, we purchased a paraffin block trimmer. It saves time and the stress on finger joints compared to scraping cassettes daily. No matter how careful we were during embedding to keep excess paraffin off cassette ed

[Histonet] Re: squamous cell contamination on slides

l, then dexterity is not lost. Good luck Gayle Callis HTL/HT/MT(ASCP) *** We have that problem mostly during the winter months when our hands get dry. U

[Histonet] Re: Decalcified bone eosin overstaining

What Hazel is saying is true but with acid decalcifiers i.e. formic, nitric or HCl. These acids damage nucleic acids aka acid hydrolysisand if the decalcification is not controlled by endpoint testing, then the nuclei will appear pale and in some cases, don't stain at all ("over-decalcification").

[Histonet] At long last, a Murine CD4 monoclonal antibody that works on FFPE tissues

Dear Histonetters, This antibody is going to be very welcome for those working with NBF fixed paraffin embedded murine tissue.It is a rat antiMouse CD4 for FFPE tissue. For those interested go to Affymetrix/eBioscience, Rat antiMouse CD4, clone 4SM95 (L3T4/Ly-4, Catalog number 14-9766.

[Histonet] Re: Processing whole rat heads

For staining, avoid overly vigorous rinsing as the softer brain can still dislodge from bone and/or fold over. Good luck and send photos to histonet when you have results. Gayle Callis HTL/HT/MT(ASCP) ___ H

[Histonet] Re: antibody suggestions CD8, FAP, Cd11b, CD11c

You Wrote: I need your help to find good antibodies for IHC on mouse and human FFPE tissues like normal tissues and tumors. Please share any information you think might be helpful. CD8 for IHC on mouse FFPE spleen or other lymphoid organs. FAP (Fibroblast Activated protein) for IHC on m

RE: [Histonet] RE: paraffin

suggest in front of the processors too. Gayle Callis HTL/HT/MT(ASCP) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Esther C Peters Sent: Wednesday, August 13, 2014 7:18 PM To: Walter Benton; Algeo

RE: [Histonet] Weight Loss/Weight Gain Decal _Histonet Digest, Vol 129, Issue 18

Re: RE:On the lighter side... (Barry Rittman) 13. Re: Weight Loss/Weight Gain decalcification endpoint test (gayle callis) 14. Consult for taking abs from RUO to IVD (Patsy Ruegg) 15. RE: On the lighter side... (susan.wal...@hcahealthcare.com) 16. RE: Cytospin validation (Jamal)

[Histonet] Liquid cover glass for H&E stained cell culture in 6 well plates; toluidine blue stain for cell cultures

or making up the stain, staining method and preferred fixative would be appreciated. You can send information privately if you wish. A challenge to be sure Thanks Gayle Callis HTL/HT/MT(ASCP) ___ Histonet mailing l

[Histonet] Re: Weight Loss/Weight Gain decalcification endpoint test

Trevor and Jennifer, This method originally came from Mawhinney et al. Control of rapid nitric acid decalcification J Clin Pathol 1984 37:1409-1415 and was cited by Cathy Sanderson (Mayton)in a publication using EDTA, found in Biotechnic and Histochemistry. Mawhinney acutally did a chemical

RE: [Histonet] Gayle Callis, You Made Our Monday Morning!!

Still chuckling and glad I provided some unintentional amusement in that lengthy post. Gayle Callis ;) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jones, Laura Sent: Monday, August 04,

[Histonet] Re: Schiff's storage temperature - long reply

Dear Histonetters, At the risk this may be more information than you need. My fascination with PAS and Schiffs reagents prompted the following. Our Schiffs reagent, prior to available commercial products, was made in house. We made Lillies Cold Schiffs solution stored at 4C accordin

[Histonet] Request for copy of old Technicon operation manual

Dear All, A friend is donating her old Technicon to be used in another country, and needs a copy of the operation manual. If anyone still has this, could they send a scanned pdf copy to me asap.I will forward it to person in need Thank you in advance Gayle Callis HTL/HT/MT(ASCP

[Histonet] Re: VIP 1000 charcoal filters

ly, but careful opening and then resealing with duct tape might work. However, first VIP charcoal filter containers were made of the same plastic as the solvent containers and not easily damaged during a change. Good luck Gayle Callis

[Histonet] Re: OCT embedding problems

fixation was popular for H&E if it wasn't for rapid diagnosis. Our frozens looked very much like FFPE unless there was incorrect, slow snap freeze causing freezing artifact. Gayle Callis HT/HTL/MT(ASCP) ___ Histonet mail

RE: [Histonet] Staining FFPE with biotinylated SNA - how to block?

Vector is a lectin expert. For those doing IHC with anti-lectin, antigen retrieval may be needed per James Watson reply and is included in the Lectin IHC protocol I have.I am presuming he was doing true IHC for his lectin work. Gayle Callis HTL/HT/MT(ASCP) -Original Message

RE: [Histonet] Mouse Joints

block after surface decalcification. This will take time for each block as you would have to repeat this each time you want to cut further into the sample. 2. If you have a tungsten carbide knife, you could try sectioning with the TC knife, and not surface decalcify. Good luck Gayle Callis H

RE: [Histonet] Treatment of calcified human arteries

Dear Galina, Formic acid, buffered that contain either sodium citrate or sodium formate that have an approximate 4.5% formic acid concentration (yes, I did the calculation one time) and as made from 90% stock formic acid. You can buy these decalcifying solutions from a vendor, but look at

[Histonet] Caveats Re: Bouins in microwave for Massons trichrome

Dear Histonetters, Microwaving Bouins can be an absolute disaster as experienced by me. It went all over the interior of microwave (borrowed one at that!) and created an unbelievable mess of picric acid that then dried out, and formaldehyde going into every nook and cranny of the microwave

[Histonet] Need help with a publication reference

Dear Histonetters, If anyone has an original copy of Woods and Ellis, Laboratory Histopathology: A Complete Reference, 1994 Churchill Livingstone, I would like to know the chapter and page numbers for Anthony S-Y Leong, " Fixation and Fixatives" chapter. Title of book, publisher, date and IS

[Histonet] Re: Activated charcoal for VIP 3000?

nd/or do a Google search. Gayle Callis HTL/HT/MT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Re: Filtering stains

for introducing dry chemicals to a container as wide ends will cause splashing. Gayle Callis HTL/HT/MT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] CD4 and CD8 mouse tumor IHC, thanks..

Good luck Gayle Callis HTL/HT/MT(ASCP) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Erickson, Jamie E Sent: Monday, December 09, 2013 12:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet

[Histonet] Re: Questions about IHC on Frozen Sections

and Brett presented, but it does drive home some points. Take care Gayle Callis HTL,HT/MT (ASCP) -Original Message- I agree with Liz, We usually fix with acetone/ethanol 5-10 min then go right into buffer, but occasionally use 2.0% NBF for some

[Histonet] Re: ImmEdge Pap Pen problems

e pens for both deparaffinized slides (well dried around section) and air dried frozen section (on dry slides). Good luck Gayle Callis HTL/HT/MT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthw

[Histonet] RE: goat anti Rabbit Alexa 594 woes.

You wrote: I would like to get your keen ideas about two issues I am facing now in my immunohistochem. procedure: 1- I am using goat anti rabbit Alexa 594 (red) as secondary antibody for detecting C-Fos in rat nervous system. When I am looking at the slides, it seems always I have some res

RE: [Histonet] Isopentane storage-the polling is open

(supported on a platform) in a styrofoam box for snap freezing multiple tissue blocks (as many as 40) in a tissue dissection/collection session. 1. If you have to have it in lab without an explosion proof refrigerator or freezer, store at RT in flammable cabinet. Gayle Callis

[Histonet] Re: Holes in paraffin, remedies

us always doing a 1 minute running water rinse after hematoxylin, acidic solution, and bluing.If you don't have running water rinsing, at least change your water rinses before each staining run. This all sounds very picky, but our H&E staining was very suc

[Histonet] Re: Oil Red O and Mayers

rs? It could be outdated or needs changing? Also, after final tap water rinse, we coverslip without an extra deionized water rinse. Good luck Gayle Callis HTL/HT/MT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.

[Histonet] FW: CD4 CD8 Zinc Tris PE staining

RT on air dried frozen sections then into buffer from this solvent fixative (sections were not alllowed to air dry again after this fixaton). As for papers you have seen, can you give the references please? Gayle Callis HTL/

RE: [Histonet] eosin in the processor

The only immunostaining any residual eosin in tissue might affect is if you do immunofluorescence. Eosin does fluoresce. Others can address any effect on IHC. Gayle Callis HTL/HT/MT(ASCP) Bozeman MT -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] Looking for US Distributor for biological dyes

Amy, Cellpoint Scientific. You have to call them for information on what you want, or a catalog? Telephone: 800-999-9734 or email: i...@cellpointscientific.com They also have brain matrix devices for rodents, etc., but no website other than contact information. Gayle Callis HTL/HT

[Histonet] Rabbit/Mouse Polymer Detection Kit Recommendation?

Biocare and Golden Bridge International have kits.I believe Vector can be added to this group too. Gayle Callis HTL/HT/MT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo

[Histonet] RE: Wrinkle Out Water Bath

the antigens are still protected by the paraffin in the tissue section. Gayle Callis HTL/HT/MT(ASCP) Bozeman MT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Re: Changing dynamics in histotechnology

Well, this honey has been on the same page since the EARLY 1960'S. I crossed over from the MT side into histology and never looked back. It was obvious very early on that histology was far more interesting than working as an MT, poor pay or otherwise. Way back in the Dark Ages, our MT traini

[Histonet] RE: zinc fixative

urpose of this fixative, I wouldn't want a sniff of formalin from NBF carry over into ANY of the solvents. Others may have more suggestions on this. I am not sure what you are using zinc fixative for, but presume it is for CD markers. Good luck, Gayle Callis

[Histonet] Re: DAPI stained smeared nuclei

to RT? Tossing out a lot of things here. Take care Gayle Callis HTL/HT/MT(ASCP) Bozeman MT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] JB fixative

Hi All, You wrote: I use a Zinc fixative called JB fixative for tissues that I want to stain for CD antigens(see attachment). Does anyone know if this fixative kills all microbes and prions? You did not say what species you are working with? The answer to your question is no for

[Histonet] Re:Glucose Oxidase peroxidase block that works for eosinophils

is slow, continuous production of H2O2 removes/blocks peroxidase and pseudoperoxidases in tissues/cells. I had feedback that it worked on paraffin sections with stubborn endogenous peroxidase. Good luck Gayle Callis ___ Histonet

[Histonet] Re: Unsubscribing problems for the amusement with a touch of seriousness for all

Dear Histonetters particularly those irritated with people who incorrectly unsubscribe from this list, After participating on Histonet since its very beginning, it is noted that incorrect unsubscribing (and other infractions) from the list has been a continuous problem from the start. Th

[Histonet] Immunofluorescence in the clinical laboratory, some questions

Dear Histonetters, I know that immunofluorescence has been done for decades on renal biopsies, but am curious if laboratories are using IF more these days? If so, I would be very interested to talk to you one on one about this as I have more questions on why you deviate from standard chromog

RE: [Histonet] Xylene Substitute for Counterstain Clearing

. Good luck! Gayle Callis -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, May 11, 2012 12:23 PM To: histonet@lists.utsouthwestern.edu; Andrew Coleman Subject: Re: [Histonet

[Histonet] Re: Massons Trichrome on decalcified bone

Dear Sara, You wrote: I work at an Orthopaedic research lab and I have been having some trouble getting our trichromes to work on our bone. I have tried a masson's, gomori, and goldner's and in all cases the bone stained red. Our bone is arrive to our lab frozen, we fix them in formalin

[Histonet] RE: Nocardia sp in FFPE

Jeanine, I always recall what the third edition AFIP manual (green) had to say about staining Nocardia successfully. Unfortunately, I didn't see an antibody for Nocardia. Several stains work and maybe have to do a combination of stains correlating results using adjacent sections?

RE: [Histonet] Gram Stain

They have excellent staining kits too. Gayle Callis HTL/HT/MT(ASCP) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Giroux, Stacy Sent: Monday, March 12, 2012 11:36 AM To: his

[Histonet] Re: undecalcified bone IHC

Jeff, It is most certainly possible to do IHC on undecalcifed bone sections embedded in PMMA although not the easiest task. Sectioning is done on a microtome that is powerful enough to cut the plastic and using tungsten carbide knives. The key is total removal of the plastic from MMA embed

Posting photographs RE: [Histonet] microtomy

observed. Harder paraffin helps for thin sections but one should be able to section at 2 um without difficulty on a properly adjusted microtome, a sharp disposable blade and using any modern day paraffin. Gayle Callis HTL/HT/MT (ASCP) Bozeman MT -Original Message- From: histonet

[Histonet] Re: PAMS Jones Basement Membrane

Stanley Shapiro, where he used freshly made 0.5% periodic acid via private email. The nuclei will pick up some silver, but one should be able to discern nuclei from basement membranes on 1 to 2 µm sections. Good luck Gayle Callis HTL/HT/MT(ASCP) Bozeman MT You wrote

RE: [Histonet] Cutting on an interview

I find the wording "cutting on an interview" interesting. It could mean cutting out e.g. leaving and not doing the interview. Cutting = sectioning?! Sorry if these appear to be a "cutting" remarks. Gayle M. Callis HTL/HT/MT(ASCP) -Original Message- From: histonet-boun...@lists.ut

RE: [Histonet] saffron vs. safran du gatinais

Beth, In the past, people replied to Histonet with the suggestion to buy saffron aka safran du gatinais from a grocery store spice section. Depending on where you are located e.g. a bigger city, try to find a store that sells spices from India may have the freshest saffron. However, you won't

[Histonet] Nail Polish sealant

You wrote: We are currently starting up some IHC on frozen tissue sections. After staining with different fluorescent antibodies, we end with applying DAPI w/Prolong gold and then coverslipping. We would like to seal the coverslip so that we can keep the slides longer. Any suggestions on where

RE: [Histonet] Re: Histonet Digest, Vol 97, Issue 21

This same clone for Rat CD31 still sold by BD Biosciences/Pharmingen/Invitrogen, also Serotec, ABCAM and a company called Research Diagnostics found in the IHC World protocol. The latter protocol used Proteinase K digestion. Gayle Callis -Original Message- From: histonet-boun

[Histonet] RE: mouse antiRat CD31

Several sources for mouse antiRat CD31 (PECAM) were found by doing a simple Google search with keywords mouse antiRat CD31 immunohistochemistry. You can buy this monoclonal from Serotec, BD Biosciences and probably eBiosciences plus other companies that specialize in rodent antibodies. As for pr

[Histonet] Re: Woelcke"s Myelin Sheath method

accessed online by NSH members free of charge. If you go to Google Scholar, you can pick up this publication Cereb. Cortex (1997) 7 (2): 166-177. doi: 10.1093/cercor/7.2.166 Or merely cut and paste the whole DOI link into Google to get free pdf/full text article. Gayle Callis HTL/HT/MT

RE: [Histonet] Tissue left in processor

processing is finished and schedule accordingly. Gayle Callis HTL/HT/MT(ASCP) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Thursday, October 06, 2011 9:45 A

[Histonet] RE: DAPI on thicker sections, problem with gradient

Carmen, You wrote: Good morning: I am Carmen Garcia from the Faculty of Medicine of the University of Valencia, Spain. I usually use your vectashield mounting medium for fluorescence with DAPI (H-1200) and I never have any problem. But now I am working with 50um frozen sections a

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