Re: [Histonet] Help to interview new employees for the first time

I'm also a fan of having any fellow employees available ask questions. I have my staff limit it to histology related subjects, but my thought is if they are going to be the ones working directly with them, it helps to have their opinions. Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent

Re: [Histonet] CAP Requirements for Scales and Balances

Yes, there are CAP requirements, and I believe they are in either the General checklist or the All Common checklist.  I think the requirement is for calibration once a year.  Laurie Redmond -Original Message- From: Piche, Jessica via Histonet To: histonet@lists.utsouthwestern.edu

[Histonet] Alopecia

Does anyone have an Alopecia policy that they would be willing to share?  One of our pathologists wants us to exhaust the block/30 slides when cutting these cases.  Is this normal practice? Thanks, Laurie Redmond ___ Histonet mailing list Histonet

Re: [Histonet] Bleach Regs

We don't store anything under the sinks in our lab per CAP regulations.  I do not understand why, nor do I have the specific checklist question at my fingertips, but we always make sure everything is removed from under the sinks at inspection time. Laurie Redmond -Original Message

[Histonet] PAS Stain

Does anyone else out there use warm water to rinse the PAS slides after the Schiff's reagent?  Can anyone comment on whether using warm water vs. cold water would have any affect on the stain? Laurie Redmond HT (ASCP) ___ Histonet mailing list Histonet

[Histonet] Restaining old Heamatology smears

a solution to this problem. Regards, Laurie. Mr. Laurie REILLY Histopathology Veterinary and Biomedical Sciences James Cook University Townsville Qld. 4811 Australia. Phone 07 4781 4468 Mobile 0448 957747 ___ Histonet mailing list Histonet

Re: [Histonet] TMA slides for IHC validation

I think they usually include negative tissue in the TMA's.  Check with Biomax on your specific slides. Laurie Redmond -Original Message- From: Dessoye, Michael via Histonet To: histonet@lists.utsouthwestern.edu Sent: Mon, Oct 15, 2018 10:34 am Subject: [Histonet] TMA slides for IHC

Re: [Histonet] VIP issue

4) there was a machine malfunction Good Luck! Laurie Redmond -Original Message- From: Gudrun Lang via Histonet To: histonet Sent: Thu, Oct 11, 2018 10:58 am Subject: [Histonet] VIP issue Dear all! I have a question for those, who are familiar with the VIPs from Sakura. Last time

[Histonet] Paraffin Dispenser

I'm looking for a 2.5 gallon (approx) paraffin dispenser ASAP - new or refurbished.  I need to find one that is in stock and can be shipped immediately.  Vendors are welcome to respond. Thank you, Laurie Redmond Path MD  Los Angeles, CA ___ Histonet

Re: [Histonet] Giemsa vs Diff Quik

Corporation. Laurie Redmond -Original Message- From: Joseph Esposito via Histonet To: histonet Sent: Thu, Sep 6, 2018 7:42 am Subject: [Histonet] Giemsa vs Diff Quik The laboratory I work at has been using the Diff Quik for years now as a stain for fine needle aspirates. Recently, when we tried

[Histonet] Per diem Histotech position at Path MD

are looking for a team player that is reliable and responsible.  Salary based on experience.  Candidates may send their resume to Laurie Redmond at both of the following emails:  boz...@aol.com     and    lredm...@pathmdlabs.com ___ Histonet mailing list

[Histonet] Special stains and hoods

I would like to know how many people perform (manual) routine special stains under a hood.  We do basic specials - Giemsa, AB/PAS, GMS, Trichrome, Muci - but we do them on the counter, not under a hood.  We have been told the staining needs to be done under a hood, but I have never done that in

Re: [Histonet] Microtomes

We have three Thermo 355S's.  They are nice because you can use them in the automated mode or manual mode.  All of our HT's use the manual mode, so now we just buy the manual version. Laurie Redmond HT (ASCP) Path MD -Original Message- From: Piche, Jessica via Histonet To: histonet

[Histonet] Excelsior Tissue Processor

If there is anyone out there who is using the Thermo Fisher Excelsior Tissue processor for biopsies, would you please contact me directly at my personal email?  I have a question about your processing program. Thanks, Laurie Redmond ___ Histonet

Re: [Histonet] pH of formalin

We monitor the concentration and pH of our recycled formalin weekly, but since it has been consistently within range I may start monitoring monthly. -Original Message- From: Baldwin, Kathy via Histonet To: 'histonet@lists.utsouthwestern.edu' Sent: Wed, Jun 6, 2018 11:25 am Subject:

[Histonet] Silver disposal

the silver into the 55 gallon drum since it was such a small percentage of silver. We use 10 ml of a 5% silver solution and then dilute that to 50 ml, so there is a very small percentage of silver in the solution. Thanks, Laurie Redmond ___ Histonet

[Histonet] V600E

Can anyone tell me about the V600E antibody?  Our pathologist wants to add it to our menu of IHC stains, and I've never heard of it.  Sources? Thanks, Laurie Redmond ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

[Histonet] p16 Antibody

their reagents into my own vials for my stainer?  I'm wondering if anyone else is dealing with this same issue. Are there other companies that sell the p16 antibody?  The last time I checked (which was years ago), Ventana was the only one selling this. Thanks, Laurie Redmond Path MD Los Angeles, CA

[Histonet] Histotech positions available in Los Angeles

include embedding, cutting and special stains Interested candidates may send a cover letter and resume to both emails below: David Costanzo, Director of Operations;  dcosta...@pathmdlabs.com Laurie Redmond, Acting Supervisor:  boz...@aol.com

Re: [Histonet] Slide labels falling off- Slidemate AS

rent slide (different manufacturer) to narrow down the issue. Good luck! Laurie -Original Message- From: Harris, Stefanie via Histonet <histonet@lists.utsouthwestern.edu> To: histonet <histonet@lists.utsouthwestern.edu> Sent: Wed, Mar 28, 2018 8:51 am Subject: [Histonet] Sl

[Histonet] Supervisor and per diem histotech positions available in Los Angeles

to Laurie Redmond at: boz...@aol.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Per diem histotech needed for nights, Los Angeles area

experience. Competitive salary offered. Please forward resumes to Laurie Redmond at boz...@aol.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Supervisor Position in Los Angeles

Immediate full time opening for Histology Supervisor at PATH MD Lab in West Hollywood, CA.  Monday-Friday, 4:00 am to 1:00 pm, no weekends;  Must be ASCP certified and have at least 5 years supervisory experience; HTL(ASCP) preferred, but will consider HT(ASCP), and Bachelors degree also

Re: [Histonet] mounting medium

We also would be interested in other's thoughts on mounting media. We are having coverslips coming unstuck from some of our teaching slides after 3 or 4 years. It is very frustrating when cover slipping manually and wondering how long it will last. Thanks and regards, Laurie. Mr

[Histonet] Part time IHC position in LA area

I am posting this for a friend of mine who was recently laid off.  He is a PhD, not a histotech, and has worked doing IHC for over 30 years.  He is very experienced and competent.  He is looking for a part time IHC position in the LA area. Please see all of his contact info below. Sabir Husain

Re: [Histonet] HSV I & II

-immunize and properly validate the antibody. HSV 2 could be in short supply for a while. Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 On Fri, Sep 1, 2017 at 1:12 PM

[Histonet] Histology Supervisor Position in Los Angeles CA

PATH MD has an immediate opening for a Histology Supervisor. PATH MD is a state of the art histology lab in West Hollywood and offers competitive salaries, full health insurance and a matching 401(k) plan.  Candidates must have prior experience and be ASCP registered.  Contact David Costanzo at

[Histonet] Employment

Two open Histology positions East Setauket, NY 6am-2:30pm 12am- 8:30am NY license required Please contact off line for details. lauriediane...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Fwd: A suitable negative control for BAP1

, Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

[Histonet] A suitable negative control for BAP1

, Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

Re: [Histonet] Medical/health related post

Joe and Rene, you have singlehandedly removed me from the list. Don't take yourself so seriously, you will likely live longer. -Original Message- From: Joseph Hardin via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Friday, April 29, 2016 10:39 AM To: Rene J Buesa;

[Histonet] Vegf.

Hi Amos, I have done vegf on a few different occasions for different species. For the biocare, I remember it needing a decloaker retrieval. Not sure if you are using pt link or another retrieval type. Laurie Popp, HT/HTL ( ASCP ) cm ___ Histonet

Re: [Histonet] Favorite book for histotechnology and immunohistochemistry?

applications", Taylor/Cote "Immunomicroscopy, a Diagnostic tool for the surgical pathologist". There are many others out there that do a fantastic job, these are just the ones that I use regularly. Thanks, Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 1

Re: [Histonet] Fatty breast tissue

and clearing steps will also help. Remember, though, when you calculate the formalin fixation times, you need to add the time that it was on the processor too. Good luck Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC

[Histonet] F480 antibody

Abserotec has a great f4/80 antibody. Laurie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] are you desperate to hire a BS.c graduate topic

areas have more qualified bodies than others. Just my .02 Laurie Popp BA, HT/HTL(ASCP)cm ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Glutamine synthetase staining

Hi Linda, What about Cleveland clinic? Regards, Laurie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Protocol?

Nine slides with 3 levels each, trichrome and elastic stain. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential

[Histonet] Thermo Excelsior Processor

directly to discuss this. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information

[Histonet] Histotech positions in Los Angeles

stains) required, but the main duty would be embedding. Both positions are available immediately. We are a growing lab and have a great team environment. Please forward all resumes to this email. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323

[Histonet] Over- decalcified tissue

I have tissue that was left in decal over the weekend and now has very poor nuclear staining. Is there a fix for this so that I can get better nuclear staining (other than restaining for a long time in the hematoxylin)? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd

Re: [Histonet] Nuclear Artifact

not to be the culprit. Collection procedures are not usually done by lab staff, a clinician or staff may have a great idea to make things easier but not know the downstream effects. Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte

Re: [Histonet] Animal reference material

The best that I have used in the past was humason's animal tissue techniques. I think that there are several editions out there, I used a 1979 edition. After googling it, I see that there may be a full text version available online too. Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent

[Histonet] RE: Cutting of Paraffin Blocks

Heck no, crack that whip! Give them practice tissue that isn't important so they won't be afraid of ruining something. Laurie Colbert, HT (ASCP) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adesupo

Re: [Histonet] anti-BRAF V600E (VE1) Ventana

Spring it is RUO, so it is up to you to do the appropriate LDT. The Ventana antibody is an IVD. It is also a pretty pricey antibody from either vendor. Let me know if you need any further help. Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd

RE: [Histonet] Masson trichrome and H and E

Medical Chemical Corporation has a trichrome kit with 4 oz reagents for around $60.00. Laurie Colbert, HT (ASCP) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Brown Sent: Tuesday, March 03, 2015

[Histonet] Linear Stainers

, can you share pros and cons please? Laurie King __ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any

[Histonet] Full Time HT position in Los Angeles, CA

. Proficiency in embedding, cutting and special stains is required. PATH MD offers full medical, dental, and vision benefits as well as a 401(k) plan. Interested applicants may send their resumes to: lcolb...@pathmdlabs.com Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los

Re: [Histonet] RE: A sticky subject....

Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 On Tue, Dec 30, 2014 at 8:45 AM, Marcum, Pamela A pamar...@uams.edu wrote: I am not sure where you live so this may or may

[Histonet] Prostate Needle Bx

How do others process prostate needle bx's - what kind of cassettes do you use? Do you use sponges? Do you wrap the tissue in lens paper? Do you process them on a biopsy (short) run? Our bx's always seem to be less than optimal, but we do not have problems with other small bx's. Laurie

[Histonet] Decolorizing effect on IHC stain

Is there any information available that would indicate the effect of decolorizing an HE stained slide on subsequent IHC staining / antigenicity? Thanks, Laurie Laurie Best,BS Histology and Cyto Prep Supervisor Sunrise Medical Laboratories 250 Miller Place Hicksville, N.Y. 11801 tel (631)435

[Histonet] RE: Water baths

Yes, I have always done that at any lab I worked at. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information

[Histonet] RE: back orders from various vendors

Yes, I am having this issue with one vendor in particular (which I won't mention). It has affected our operations and it is very frustrating! Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab

[Histonet] HPV ISH

What do others do for HPV ISH? Do you do the stain yourself in-house, or do you send it out? I know there used to be a stainer specifically for ISH - does this still exist and who is the vendor? I heard there is some kind of conflict between vendors and the FDA?? Laurie Colbert, HT (ASCP

[Histonet] Storage of Glacial Acetic Acid

How do you store GAA? As I recall, it should not be stored with other acids in an acid cabinet. I'm think it should be stored with flammables?? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab

[Histonet] Labeling Errors

Is there a national average or benchmark for acceptable labeling errors in Histology? Laurie Colbert HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] slide id

We do. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected

Re: [Histonet] FW: Thermo-scientific vs Ventana Benchmark XT

of money. Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 On Mon, Sep 8, 2014 at 11:31 AM, Cheri Miller cmil...@physlab.com wrote: Cheryl A. Miller HT ASCP cm

[Histonet] GHS Labeling

Does anyone know of a source that sells a poster that can be hung on the wall that shows all of the pictograms for the new Globally Harmonized System (GHS) labeling system? I would like a master list that can be referred to. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly

[Histonet] Glassware Cleaning again

Is bromocresol purple the same as bromcresol purple? The CAP question regarding glassware cleaning refers to bromcresol purple, but I ordered a powder and it is labeled as bromocresol purple. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323

RE: [Histonet] Water/Bubbles

the alcohol. Also, avoid shaking the excess xylene off of the rack when you take it off the stainer before coverslipping. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained

Re: [Histonet] Slides for Thermo Slidemate

) that does has behaved very well in our Slidemates.. Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 On Mon, Jun 23, 2014 at 3:37 PM, Laurie Colbert lcolb

[Histonet] Slides for Thermo Slidemate

What slides are others using (other than Thermo's Superfrost Plus slides) that won't jam in the Slidemate and that will produce print that does not smear? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main

RE: [Histonet] Edit protocol for Shandon Gemini Varistainer

Call Thermo and talk to Sherry Anderson at x562. She is awesome! She just walked me through some programming issues this morning. Thermo (800) 522-7270 x562 Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284

Re: [Histonet] RE: competency form

We based ours off the Michigan Society of Histotechnologist's Competency Assessment Handbook (available on their website for a nominal fee). We took some of their forms/ideas and modified them for our use. Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T

[Histonet] CAP Question, New Reagent Lot Confirmation of Acceptability

has to perform the comparison. What are other labs doing? Are the histotechs signing off on new lots? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may

[Histonet] CM1800 manual

Got the manual I asked for, thanks everybody! Laurie __ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message

[Histonet] Leica Reichert Jung Cryocut 1800

Hello all, Looking for a manual for a Leica Reichert Jung Cryocut 1800. Laurie __ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should

[Histonet] RE: Cornflaking artifact

mountant and glass coverslips, I'm sure you will not see the artifact. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon Scalise Sent: Wednesday, March 12, 2014 8:00 PM To: histonet

[Histonet] IHC Validation

If I have validated an antibody at a specific incubation time, and then later want to decrease the incubation time, do I have to re-validate?? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab

[Histonet] RE: Fungus Controls

I am in need of fungus controls, if there are still some out there. I can provide shipping costs. Thanks! Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained

[Histonet] Herpes I and II

Can anyone tell me where I can purchase the Herpes I and II antibody combined and also IHC control slides for both type I and II - on one slide. Vendors welcome to respond. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct

[Histonet] Billing for ASR's and RUO's

Do hospitals/labs get reimbursed for staining with ASR and RUO antibodies since they are not FDA-approved? What if they are validated in your own lab? Laurie Colbert, HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

[Histonet] Respirator Guidelines

Are there any CAP requirements or checklist questions (I know there are OSHA requirements) that pertain to the use of respirators? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information

[Histonet] RE: Fungus contamination

I have had fungal contamination in the Fast Green counterstain from Thermo. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged

[Histonet] Buffer pH for IHC

What does everyone use to test the pH of their buffers for IHC? Are pH strips adequate or do you have to have an actual pH meter? If you use a meter, any suggestions on a make and model? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648

[Histonet] HistoWrap Filter Paper

small specimens/curettings. The wraps can be folded to fit inside of a plastic funnel and specimens can be filtered directly into the paper. If there is similar alternative that is available, I would be interested in hearing what that is. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD

Re: [Histonet] slides with flimsy coverslip coming off

on top. Since the coverslips dissolve in acetone, we tried several things with attaching the tissue, using acetone to dissolve the coverslip, but it never worked very well. Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte

[Histonet] Nails for fungal stain

question: Would the potassium hydroxide have any bearing on the results of the fungal PAS since the nails are not fixed in formalin before going into the potassium hydroxide? Laurie Colbert, HT (ASCP) ___ Histonet mailing list Histonet

[Histonet] RE: Freezer for tissue storage

In my experience the uprights have more frost buildup problems, causing sealing problems. The chest ones I've had in the lab do less of this. Laurie -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cartun

Re: [Histonet] 2014 IHC CPT coding

Yes, we are trying to wrap our heads around this one. 24% drop for the first IHC stain done on a specimen and 41% for each one following. This will hit our IHC department hard. Perhaps even will hurt patient care. At least the 88360 came out unscathed. Patrick Laurie Celligent Diagnostics

[Histonet] RE: Amended Reports

The specimen container and requisition should always be compared before accessioning. If the name on the specimen container label and the name on the requisition do not match, we do not accept the specimen. It should be sent back to be properly labeled. Laurie Colbert, HT (ASCP

[Histonet] RE: off site frozen sections

to process and send finished slides. Laurie -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hutton, Allison Sent: Tuesday, December 03, 2013 10:16 AM To: 'histonet@lists.utsouthwestern.edu' Subject

[Histonet] RE: Stainer/coverslipper question

is extremely fast and very trouble-free. I have heard that Sakura's glass coverslipper is good. We currently use the ClearVue glass coverslipper from Thermo, and it works well. Laurie Colbert, HT (ASCP) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun

Re: [Histonet] Specimen collection/transportation

state, only certain people who are trained can clean up any spill that is over 1 gallon. Patrick Laurie On Mon, Nov 18, 2013 at 3:13 PM, LeAnn Lang ll...@aipathology.com wrote: This was the message I received this morning: (I asked for the specific documentation on the violation). I also

RE: [Histonet] Problems with HE Bleeding

You probably have water and/or alcohol contamination in your xylenes. Make sure your dehydrating alcohols after the eosin are really clean. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab

Re: [Histonet] Aspergillus tissue blocks for controls

/listinfo/histonet -- Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 ___ Histonet mailing list Histonet

[Histonet] slide drying oven

Can anyone recommend a small, forced air slide drying oven? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab ___ Histonet mailing list Histonet

Re: [Histonet] slide drying oven

Hi Laurie, Biocare has one that I have used, they call it the desert chamber pro, one of the few that is setup and designed expressly for drying slides. It works very well, and has different heating options and a timer. However, all you may need is a basic mechanical convection oven, Boekel has

[Histonet] RE: Endometrial biopsies

, but it doesn't always help. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Tuesday, October 22, 2013 4:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Endometrial

[Histonet] Punch Biopsies

well - they are hard to section and we get incomplete sections. For the most part, all of the other tissue is fine to cut. Is there a trick to cutting/processing punches? We have never had so much trouble with them! Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD

[Histonet] Validation Sign off

When validating IHC antibodies and detection kits (either initially or with new lots), does a pathologist have to sign off on the validation , or can it be his/her designee (such as a PhD) that signs off? The pathologist will be signing the IHC procedures. Laurie Colbert, HT (ASCP

RE: [Histonet] GI biopsy slides

We cut exactly as Sheila does. Laurie Colbert, HT (ASCP) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Tuesday, September 10, 2013 8:59 AM To: Elizabeth Chatfield; histonet

[Histonet] Razor Blade Holder

Does anyone know where I can purchase the little plastic holder for razor blades for trimming your blocks and what the thing is called?? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab

[Histonet] IHC using Thermo antibodies and stainer

If there is anyone out there who is using Thermo's Autostainer and also using their antibodies, would you mind contacting me offline? We are just starting up IHC's, and I would appreciate some feedback and information on a few issues I am having. Thanks. Laurie Colbert, HT (ASCP) Histology

[Histonet] Recycled Xylene

Does anyone out there have any problems with using recycled xylene to clean the tissue processor? I was just told that we should not use recycled xylene to clean the tissue processor. How about using recycled xylene for either processing or staining? Laurie Colbert, HT (ASCP) Histology

Re: [Histonet] Competency for Anatomic and Clinical Pathology at

@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869

[Histonet] p16 control tissue

We are going to be running the p16 antibody from Biocare (RUO) on skin specimens. What type of tissue should I run for a positive control? The data sheet says normal testis.. Laurie Colbert ___ Histonet mailing list Histonet

[Histonet] Block labeling

legibly, so I was wondering if there is a clever solution that someone might have found. We've tried putting stickers or labels on the back, but that falls right off. Thanks in advance for any suggestions. -- Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East

Re: [Histonet] Tissue Tek VIP 3000

http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com ___ Histonet mailing list Histonet

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