Thanks for the input on ouor antigen retrieval problem. Biocare did a little
troubleshooting for us, and things look great, at least for today.
Is your lab playing the lottery? Our is:
http://www.chicagonow.com/downsize-maybe/2016/01/sorry-gang-we-might-not-win-the-lottery-tonight/
Lester J. R
Hello 'netters, hope you all had a good weekend.
We are having some difficulties with the pressure cooker chambers we use for
antigen retrieval. What different methodologies would be recommended? If we
went to a different methodology for retrieval, would each antigen have to be
revalidated? Ho
Your 2 minutes would be better spent looking in an immunohistochemistry
textbook. A small but excellent one is Polak, J.M. and Van Noorden, S. (1997).
Introduction to Immunocytochemistry, 2nd ed. Royal Microscopical Society
Microscopy Handbooks, 37. Oxford: BIOS Scientific Publications.
You wi
Hi,
I am conducting a short 2 min survey for my science/business class
examining current trends for antigen retrieval also known as heat induce
epitope retrieval. Response will be greatly appreciated!
https://www.surveymonkey.com/s/7989LKR
Best,
Craig Vollert
Graduate Student
Department of Pharm
Lynn, I use the Decloaker and also stain for BVD, so will get in contact
with you later this afternoon.
Jan Shivers
University of Minnesota Veterinary Diagnostic Lab
St. Paul, MN 55108
On Thu, May 30, 2013 at 9:54 AM, Burton, Lynn wrote:
> If anyone out there is using a decloaker for antigen r
Is this with an older tissue sample? We don't do antigen retrieval, but we do
have a proprietary technique for tissue restoration which can help with
problematic IHC studies, especially in older FFPE tissue samples.
See here for some examples:
http://hematoxylin-eosin-tales.blogspot.com/2013/04
If anyone out there is using a decloaker for antigen retrieval, when doing ihc
staining, would you please contact me on my e-mail address? I have been
struggling with a BVD test for months. It is not working consistently and I
want to explore using this method along with our Benchmark XT stainer
Does anyone have a favorite antigen retrieval method for FFPE mouse tissues
that they would be willing to share? I have been using citrate buffer Ph6.0
with poor to moderate results. Thanks for any help!
Mike
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To those with the Biocare intelliPATH system, Is the antigen retrieval part of
the IHC automated or do you need to use the Biocare decloaker? Thankyou, Tom
Truscott
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Andrea T. Hooper
Sent: Wednesday, July 13, 2011 11:10 PM
To: Histonet
Subject: [Histonet] Antigen retrieval survey
Hi All,
I am doing a survey and will be happy to compile results and share if folks
will respond! What is your favorite antigen retrieval method and/or panel?
Buffer
Source
Citrate buffer is my first port of call.
Jim Reilly
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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Andrea T. Hooper
Sent: Wednesday, July 13, 2011 9:10 PM
To: Histonet
Subject: [Histonet] Antigen retrieval survey
Hi All,
I am doing a survey and will be
Citrate buffer
René J.
--- On Wed, 7/13/11, Andrea T. Hooper wrote:
From: Andrea T. Hooper
Subject: [Histonet] Antigen retrieval survey
To: "Histonet"
Date: Wednesday, July 13, 2011, 11:10 PM
Hi All,
I am doing a survey and will be happy to compile results and share if f
Hi All,
I am doing a survey and will be happy to compile results and share if folks
will respond! What is your favorite antigen retrieval method and/or panel?
Buffer
Source/composition
Temperature
Device
Thanks, Andrea
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Hi tistonetters,
I have to do IHC on 10µ sections. Is procedure for Antigen Retrieval same like
for 4-5µ (time and temperature)
Thanks in advance,
Naira
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Dear Histonetters,
I was wondering if anyone out there has used antigen retrieval methods to
facilitate immuno-staining of formalin-fixed skeletal muscle fibers? I am
using ALD-58 + ABC Elite Mouse kit to stain slow-tonic fibers in fresh
amphibian muscles, but need to take a look at the fiber dis
test
immediately after HIER. Maybe that is why it has not happened to me.
Just a thought!
René J.
--- On Tue, 10/20/09, Connolly, Brett M wrote:
From: Connolly, Brett M
Subject: [Histonet] Antigen retrieval question
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, October 20, 2009, 2:21 PM
Sounds like the retrieved antigen was lost rather than "reversal"
Janet
QIHC/HTL
--- On Tue, 10/20/09, Connolly, Brett M wrote:
From: Connolly, Brett M
Subject: [Histonet] Antigen retrieval question
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, October 20, 2009, 11:21 AM
Has anyone experienced a "reversal" of citrate HEIR unmasking do to a
prolonged delay in continuing the experiment after the retrieval step?
Brett M. Connolly, Ph.D.
Research Fellow, Imaging Dept.
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
tel. 215-652-2501 fax. 215-993-6803
brett_co
Dear all,
Might anyone be able to offer their advice on the best method to retrieve
antigens on paraffin embedded skin sections? I am immunostaining for
epithelial cytokeratins, and I have block-to-block variation in the
quality of the staining - some block give consistently good staining while
I am attempting to do IF on FFPE sections but the history/treatment of my
tissues (mouse tissues) is unknown. I would like to recommend to our labs a
strategy for tissue collection, length of time in NBF, type of antigen
retrieval, and so on. Would anyone (or everyone) be willing to share thei
Anjan,
I am more of a fan of steamer or waterbath than pressure cooker, in
my= hands at altitude the pressure cooker can be very harsh and make
my tissue= s fall off the slide or get chewed up badly. I know the
pressure is s= upposed to be controled by the cooker but in
hello everyone,
I wanted to know the exact stepwise protocol for antigen
retrieval using a pressure cooker. i tried using some protocol but there is no
staining. Kindly tell me the detailed protocol, like when i should place the
slides into the pressure cooker and from when
We put our plastic sections (5-6 microns in thickness) onto slides
manufactured by Scientific Device Laboratories, the "in situ plus"
slides. We then bake the sections overnight in a 60 degree oven, then
bring to room temperature before doing immunostaining. Sections will not
stay on gel coat
-06
tf
发件人： Swain, Frances L
发送时间： 2008-10-06 19:44:38
收件人： Sebree Linda A.; Patten, Nicole (NIH/NIAAA) [F];
histonet@lists.utsouthwestern.edu
抄送：
主题： RE: [Histonet] Antigen Retrieval
I work with non-decalcified and decalcified bone. After I have tried to
pressure cook it, steam it,
, October 03, 2008 12:49 PM
To: Patten, Nicole (NIH/NIAAA) [F]; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Antigen Retrieval
Try using a laboratory pressure cooker like the Decloaking Chamber from
Biocare Medical.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC
, October 03, 2008 11:28 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Antigen Retrieval
Hi-
I am new to using IHC and I need some advice on Antigen Retrieval. I
usually do it in an autoclave for 15min at 121C for FFPE human brain
tissue (in Citrate or Tris Buffer), but by the end
MAIL PROTECTED] On Behalf Of Patten,
Nicole (NIH/NIAAA) [F]
Sent: Friday, October 03, 2008 12:28 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Antigen Retrieval
Hi-
I am new to using IHC and I need some advice on Antigen Retrieval. I
usually do it in an autoclave for 15min at 121C
Hi-
I am new to using IHC and I need some advice on Antigen Retrieval. I
usually do it in an autoclave for 15min at 121C for FFPE human brain
tissue (in Citrate or Tris Buffer), but by the end my tissue looks
pretty bad and parts have even fallen off. The tissue is fixed on
SuperFrost Plus Slid
Are your PAP smear slides fixed in alcohol? If so, you probably don't need
antigen retrieval.
Richard
Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596
(860) 545-0174 Fax
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of hao
zhang
Sent: Sunday, 14 September 2008 1:12 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] antigen retrieval for
[Histonet] antigen retrieval for 5 year pap smear slides
Hi, everyone
I want to do IHC from a 5 year old pap smear slides for pan-keratin
stain.
I tried many methods of antigen retrieval protocols (10 mM citric
buffer, ph 6; 1 mM EDTA, Ph8; 10 mM Tris buffer, Ph 10; ) and heat at
95C for 40 minute
Usually PAP smears are NOT fixed with NBF so they should NOT be treated with
HIER before doing IHC. If you have some spare smear do IHC directly (after
destaining).
René J.
--- On Sat, 9/13/08, hao zhang <[EMAIL PROTECTED]> wrote:
From: hao zhang <[EMAIL PROTECTED]>
Subject: [Histo
Hi, everyone
I want to do IHC from a 5 year old pap smear slides for pan-keratin stain.
I tried many methods of antigen retrieval protocols (10 mM citric buffer, ph
6; 1 mM EDTA, Ph8; 10 mM Tris buffer, Ph 10; ) and heat at 95C for 40
minutes. I also used Triton x 100 (0.2%) pretreatment 10 minut
Brian,
I haven't seen your procedure for antigen retrieval before. I usually see
heat/citrate buffer or some kind of protease treatment. It seems pretty
straightforward. Have you ever used this procedure for keratins or
basement membrane proteins?
Brian wrote:
I have performed antigen retrieva
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