Martha,
We reported our study of 15 brands of adhesive slides for "wash off" and
found little difference among the different slides when well-fixed cell
culture material was examined.
On the other hand, poorly-fixed breast cancer tissues did appear to adhere
more strongly to some slides than
It was brought to my attention that we had significant washing on 3 of 8 bone
marrow clot sections the other day; this is not the first time so we would like
to get to the bottom of this. We use positively charged slides and all 8
cases were cut and run the same morning but allowed to air
Our Histology Techs also assist with Bone Marrows in CT, though there is little
to do. We collect the aspirate in Heparin Tubes and the core in Bouins. We
bring it back to the lab and make our smears from one of the heparin tubes.
The other tubes go for special studies. I'd like to think
Can someone please recommend a good place to order customizable bone marrow
biopsy kits?
We want foam for specimen containers, tubes, and want to add our own
products. Not premade completely.
Thank you,
JB
___
Histonet mailing list
To: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] [Histonet] bone marrow clots
Hi all,
Question: Does anyone produce beautiful, well fixed bone marrow clot blocks
that has a procedure they would be willing to share? Do you all use the
specimen from the EDTA tube after prepping the smears
Hi all,
Question: Does anyone produce beautiful, well fixed bone marrow clot blocks
that has a procedure they would be willing to share? Do you all use the
specimen from the EDTA tube after prepping the smears? Any help would be
greatly appreciated!
Thank you,
Noëlle
Noëlle Linke, MS,
Hi All,
Are you allowed to bill an 88305 for both the aspirate and the biopsy on bone
marrow cases?
Thank you,
Lacie
Lacie Algeo, HTL (ASCP) MBCM
Histology Supervisor
Providence Sacred Heart Medical Center Laboratory
101 W 8th Avenue
L-2
Spokane, WA 99204
509-474-4418
FAX 509-474-2052
How are you billing for bone marrow bxs. we do irons on smear and core plus
clot. should we bill for all iron stains? also we do a wrights stain.
Anita Dudley
Providence Hospital
Mobile, Al
___
Histonet
I am looking for a Bone Marrow procedure that uses Immunocal as the
decalcifier. I am particularly interested in how long they fix in
Immunocal and if heat is used in any way . Our lab just started using
this product because we are under the impression that it gave better ISH
results.
Thank-you
...@ihctech.net
Date: Mon, 17 Nov 2014 14:05:28 -0500
From: carolyn.barn...@va.gov
To: Histonet@lists.utsouthwestern.edu
CC:
Subject: [Histonet] Bone Marrow processing using Immunocal
I am looking for a Bone Marrow procedure that uses Immunocal as the
decalcifier. I am particularly interested
Over the past several years we have had some questions by our
hematopathologists regarding the quality of the trephine blocks. The biggest
concern has been that the sections seem to have a fragmented appearance which
we have determined in the past to be areas where the lipid cell borders
Has anyone experienced poor staining of bone marrow smears that were sent from
a doctor's office to a central laboratory? We have some cases of poor staining
that we are thinking may be from fume contamination from the fixative bottle.
Has anyone experienced anything of this nature? Thank you
Hi All , I was wondering if anyone had any advise on getting clots and cores to
stay put? We are using plus slides and giving the slides extra oven time and we
are still having issues. So if anyone has some histomagic up their sleeve
regarding this issue it would be appreciated.
Ginny Miller
I sent out a request for information a while back and am going to ask again
from a different approach.
I am finding problems with embedding blocks of bone marrow particles with or
without blood clot.
Does anyone have a special technique they would like to share to concentrate
the particles
on the
quality of the slides.
Cathy
Kelowna, B.C.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shea's
Sent: Saturday, June 1, 2013 4:26 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] bone marrow
I am also interested in how others are processing bone marrow aspirates.
Currently, we let the aspirate clot on a watch glass for about 20 minutes,
gentle slide it onto filter paper, roll it around to get rid of the excess
fluid, place the clot between sponges, and process as usual.
However, I
Can anyone share with me their process for processing bone marrow aspirations
and embedding them in paraffin blocks. Currently ours are being spread out
throughout the entire base mold and makes it cumbersome to screen. Has anyone
used histogel or making a button and embed that instead of
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jordan Phillips
Sent: Tuesday, May 28, 2013 7:56 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Bone Marrow Issues
HI everyone. We are currently having problems with all of our bone marrows,
mainly clots, washing off the slides. We
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jordan
Phillips
Sent: Tuesday, May 28, 2013 7:56 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Bone Marrow Issues
HI everyone. We are currently having problems with all of our bone
marrows
HI everyone. We are currently having problems with all of our bone marrows,
mainly clots, washing off the slides. We fix our bone marrows in Zenkers. We
use positive charged slides, put the slides in the oven for a minimum of 2
hours and hand depar with no agitation. When we get to the
Try hydrating the slide in 95% then distilled before staining. The iron stain
is made in water so hydrating to water should help the stain transfer better.
Hope this helps :)
Cassandra Davis
cda...@che-east.org
302-575-8095
Saint Francis Hospital
Saintfrancishealthcare.org
Saint Francis
Hello, We are currently having problems with our bone marrow aspirate slides
for iron stains which we run on the Ventana nexes special stainer. The RBCs
look crenated or damaged. We currently fix them 5 minutes in methanol then air
dry before running them on the stainer. Any suggestions?
Jason
What fixative and decal solution is everyone using for bone marrow specimens
which will have subsequent IHC and Kappa/Lambda ISH staining? We are currently
using B+ fixative and Decal A (formic acid and formaldehyde). Our pathologists
demand a quick turn around time, and are willing to
I fix in NBF at pH7 exactly and decalcify with EDTA
René J.
From: Clare Thornton cthorn...@dahlchase.com
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Sent: Thursday, February 14, 2013 9:22 AM
Subject: [Histonet] bone marrow specimens
What fixative and decal solution
Does anyone have a protocol on how to fix and process a bone marrow aspirate to
paraffin?
Thanks,
Tim Coskran
Pfizer
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
on the slide, the whole slide is
marrow.
Good luck!
Ashley
Message: 5
Date: Fri, 4 May 2012 16:54:57 +
From: Coskran, Timothy M
timothy.m.cosk...@pfizer.commailto:timothy.m.cosk...@pfizer.com
Subject: [Histonet] bone marrow aspirate
To:
histonet@lists.utsouthwestern.edumailto:histonet
What is the procedure for collecting and processing bone marrow bx? How much
time should it be placed in DECAL??
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
What's up Tena!
Long time. The bone marrows will be fixed in B-5.
Dawud
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Hello Histonet!
Does anyone have an HE automated stainer protocol for bone marrows that they
wouldn't mind sharing? I'm looking at different protocols. It would be greatly
appreciated. Thanks.
Dawud
___
Histonet mailing list
dmlongo...@ecrmc.org
Subject: [Histonet] bone marrow biopsy
To: Histonet@lists.utsouthwestern.edu
Histonet@lists.utsouthwestern.edu
Message-ID:
12b71261212be94bb9fb7735484b9fa1014...@exmbx01.ecrmc.ci.el-centro.ca.us
Content-Type: text/plain; charset=us-ascii
Hello Histoland,
I read
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana
Martinez-Longoria
Sent: Thursday, March 24, 2011 11:01 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] bone marrow biopsy decals
Our pathologist wants
Our pathologist wants to know why Formical-4 is the best decal for bone marrow
biopsies, since we heard about in Histonet? I have experimented with four
different decals: Rapid-Immuno, Formical-4, Nitric Acid, and HCL\Formic Acid
and we are still not sure which will be the best decal. Also we a
Hi Diana,
I recently made a histonet posting Great B5 substitute which also
addresses BM decal. Since there are a number of companies who sell
decal solutions with similar names, I am not sure you tried the
products from BBC Chemical. It is best to use both products
together. My post
Hi All,
I would appreciate some insight as to how most labs are treating bone marrow
smears, in regards to fixation. I am not embarrassed to say that it has been
awhile since I worked with them. With the smears are most labs air drying,
methanol (alcohol fixation) or spray fixation (pap
Either air dry → methanol (most labs) or air dry → PAP fixative (I always
preferred methanol).
René J.
--- On Wed, 3/2/11, Debra Siena dsi...@statlab.com wrote:
From: Debra Siena dsi...@statlab.com
Subject: [Histonet] bone marrow aspirations
To: histonet@lists.utsouthwestern.edu histonet
...@statlab.com
Subject: [Histonet] bone marrow aspirations
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Date: Wednesday, March 2, 2011, 10:43 AM
Hi All,
I would appreciate some insight as to how most labs are treating bone marrow
smears, in regards to fixation
We always air dried them and fixed them in methanol, otherwise they washed
off.
Debra Siena dsi...@statlab.com
Sent by: histonet-boun...@lists.utsouthwestern.edu
03/02/2011 07:49 AM
To
histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
cc
Subject
[Histonet] bone marrow
Hi All,
It appears that air drying and then methanol fixation is the method most labs
are using on bone marrow smears. Thanks for all your help, I do appreciate all
the responses. Best Wishes
Debbie Siena HT(ASCP)QIHC
Technical Manager | StatLab Medical Products
407 Interchange St. |
[mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of Goodwin,
Diana
Sent: Monday, February 21, 2011 11:40 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Bone marrow charges
Greetings, Histonetters.
How many times can I charge 88313 for a bone marrow case
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Bone marrow charges
Greetings, Histonetters.
How many times can I charge 88313 for a bone marrow case that has an Iron stain
on 2 separate blocks (one for the bx and one for the aspirate clot) and also on
1 smear made from the aspirate clot, a PAS
Greetings, Histonetters.
How many times can I charge 88313 for a bone marrow case that has an Iron stain
on 2 separate blocks (one for the bx and one for the aspirate clot) and also on
1 smear made from the aspirate clot, a PAS on the 2 blocks, a Trichrome and a
Retic on the bx. block, and a
I sent out an email around two months ago regarding a problem with our bone
marrow trephines. Our pathologist says that many of the trephines appear to
be fragmented and although this has not prohibited determining a diagnosis it
is still a problem. Currently we use AZF as a fixative and
Need Histonet help. Hematology recently purchased a new blood smear
stainer that is producing variable staining. It is especially bad with
the bone marrow smears, even when it is run through twice as was the
norm with the old stainer (now gone).
Until this is resolved, does anyone have a GOOD
Hello all,
Need Histonet help. Hematology recently purchased a new blood smear
stainer that is producing variable staining. It is especially bad with
the bone marrow smears, even when it is run through twice as was the
norm with the old stainer (now gone).
Until this is resolved, does anyone
will find this
also with alcohols
From: histot...@imagesbyhopper.com
To: histonet@lists.utsouthwestern.edu
Date: Mon, 26 Apr 2010 23:26:43 -0400
Subject: [Histonet] Bone Marrow Clots - falling off slides
Hi Histonetters!
We have started to have an issue with our bone marrow clots falling
off
Message-
From: Lynette Pavelich [mailto:lpave...@hurleymc.com]
Sent: Tuesday, April 27, 2010 6:56 AM
To: histot...@imagesbyhopper.com; histonet@lists.utsouthwestern.edu; MARY
HODGES
Subject: RE: [Histonet] Bone Marrow Clots - falling off slides
When I start losing tissue on the slide, I have found
, April 27, 2010 6:56 AM
To: histot...@imagesbyhopper.com; histonet@lists.utsouthwestern.edu;
MARY
HODGES
Subject: RE: [Histonet] Bone Marrow Clots - falling off slides
When I start losing tissue on the slide, I have found that it is
usually a
processing issue. Is the clot too thick? Try cutting
!
Michelle
-Original Message-
From: Lynette Pavelich [mailto:lpave...@hurleymc.com]
Sent: Tuesday, April 27, 2010 6:56 AM
To: histot...@imagesbyhopper.com; histonet@lists.utsouthwestern.edu;
MARY
HODGES
Subject: RE: [Histonet] Bone Marrow Clots - falling off slides
When I start losing
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
histot...@imagesbyhopper.com
Sent: Tuesday, April 27, 2010 7:33 AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Bone Marrow Clots - falling off slides
Mary,
Thanks for the suggestion. We do check
I have to agree with Lynette. Good solution as well ...change only one thing at
a time.
We occasionally have the same problem and the BM clots are the only tissues in
the entire batch to fall off (It is usually the blood in the center of the
section, the rim stays attached, which is the clue
Hi Histonetters!
We have started to have an issue with our bone marrow clots falling off the
slides. We are using plus slides, making sure they drain well (just like
our other slides), but when we stain them routinely, we are getting a fair
amount of tissue coming off the slides.
It has been
: [Histonet] Bone Marrow Clots - falling off slides
Hi Histonetters!
We have started to have an issue with our bone marrow clots falling off the
slides. We are using plus slides, making sure they drain well (just like
our other slides), but when we stain them routinely, we are getting a fair
amount
I'm in a bit of trouble. I work in the south of Brazil (Porto Alegre) for some
years and now in my hospital a lot of bone marrow biopsies are being performed
(leukemia and lymphomas). With decalcification with strong acids (nitric) I'm
getting very poor results with the immunohistochemistry.
-7210
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marinez
[mbba...@uol.com.br]
Sent: Wednesday, April 21, 2010 4:14 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Bone marrow trephine
I am using Fix-All from Surgipath for bone marrow fixation. I empty the
syringe into the fixative as soon as it is handed to me (no messing around with
clots). I fix for 1-2 hours in Fix-All then transfer to a screen cassette and
our normal processing with 10%NBF. The pathologist is very
Acetic Zinc Formalin
Disclaimer: The information in this message is confidential. If you are not
the intended recipient, do not disclose, copy, or distribute this message, and
please immediately contact the sender.
___
Histonet mailing list
Good afternoon everyone,
In past years our lab used B-5 fixative on bone marrow biopsies for better
immunostaining.
With the chemical hazards of B-5 we switched to AZF fixative and have been
using this for the past few years. I am just curious what everyone else is
using for fixative on their
Buffered 10% NBF, but with the pH exactly at 7.0, checked for each case. IHC is
perfect.
René J.
--- On Tue, 3/2/10, Amy Farnan farn...@nehealth.com wrote:
From: Amy Farnan farn...@nehealth.com
Subject: [Histonet] bone marrow fixative
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, March
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] bone marrow fixative
Good afternoon everyone,
In past years our lab used B-5 fixative on bone marrow biopsies for better
immunostaining.
With the chemical hazards of B-5 we switched to AZF fixative and have been
using this for the past few
We have recently been receiving bone marrow specimens where the syringe
has been heparinized before the aspiration is retrieved. We are having
problem getting the clot sections to stay on the slide. Could the
heparinization be causing this problem, and if so, is there a solution?
Thank you
Gary, absolutely the heparin is the problem. We (histologist) would
participate in the bone marrow procedure to make slides immediately with the
unheparinized sample. If someone is making those slides for you, they need to
use an unheparinized specimen. Of course, that makes the task of
Nice tip!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lynette
Pavelich
Sent: Thursday, February 04, 2010 2:19 PM
To: histonet@lists.utsouthwestern.edu; Gary Martin
Subject: Re: [Histonet] Bone Marrow
Good morning,
I am currently looking at the possibility of running bone marrow cores
and clots on our Peloris. Has anyone had success with this, and if so
would you be willing to share your protocol? We use xylene on our runs.
Debra Ann Ortiz
Chief Medical Technologist
The University of
A good tissue processor can handle those specimens without the need of any
protocol modification.
René J.
--- On Tue, 1/26/10, debra.or...@uchospitals.edu debra.or...@uchospitals.edu
wrote:
From: debra.or...@uchospitals.edu debra.or...@uchospitals.edu
Subject: [Histonet] Bone marrow
I am looking for some info on technique at bone marrow procedures?
Are aspirates being dropped from the syringe onto the slides or onto a
petri dish and only marrow with spicules transferred to slides or
something else? ??
Is the syringe heparanized??
Thank you in advance
Anna
The current protocol that we follow here is as follows: Two green tops are
obtained first thing for Flow and Cyto followed by eight smears, from that same
syringe then draw air in and place on its side allow a clot to form. From the
bone bx make a touch prep rolling the bone between two slides
Same here!
Mike
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Lecorchick, William
Sent: Monday, September 21, 2009 12:50 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] bone marrow
Has anyone had problems with bone marrow clots where the inside is well
preserved and the cells around the rim are staining lighter and some rbc's may
be lyced. We use the thrombin clot method.
Thanks,
Jimmy
Jimmy Lofton, M.S., HT,CT(ASCP)
Manager Histology Laboratory
Holy Cross
Use the same thing you would use for a blood smear: methanol 5 minutes and air
dry it.
René J.
--- On Mon, 3/9/09, Vanessa J. Phelan vjp2...@columbia.edu wrote:
From: Vanessa J. Phelan vjp2...@columbia.edu
Subject: [Histonet] Bone marrow aspirates from mouse
To: histonet
Hi all. I have a pathologist complaining about naked nuclei
in his bone marrow smears. It's fairly random, some are great and
some are naked. Anyone heard of this and have any idea what
may be causing this nakedness?
Any input would be helpful.Thank you.
Sheila Haas
Laboratory Supervisor
Micro
In bone marrow smears it is caused by improper fixation. In the same way that
Cabot rings in red blood cells are caused by improper fixation with methanol.
René J.
--- On Thu, 2/12/09, Sheila Haas micropathl...@yahoo.com wrote:
From: Sheila Haas micropathl...@yahoo.com
Subject: [Histonet] Bone
] On Behalf Of Georgine
Whitman
Sent: Friday, November 14, 2008 9:51 AM
To: [EMAIL PROTECTED]
Subject: [Histonet] Bone Marrow smears fixation
we are trying to do an iron stain on our bone marrow smears on the Ventana
Nexus. We are having trouble with the specimen staying on the slide. I
personally
We are currently using 10% formalin fixation on our bone marrow cores. We fix
for 2 hours minimum prior to decal. We are using Immunocal from Decal Corp.
for 2-4 hrs followed with processing overnight in VIP. Cores are still crunchy
upon sectioning and we are doing surface decal for up to 30
Suite 309
Charleston, South Carolina 29425
Tel: (843) 792-6353
Fax: (843) 792-8974
-Original Message-
From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Cynthia Robinson
Sent: Thursday, October 30, 2008 11:44 AM
To: histonet
Subject: [Histonet] bone marrow biopsies
We
]
[mailto:[EMAIL PROTECTED] Im Auftrag von Angela R.
Murray
Gesendet: Mittwoch, 24. September 2008 19:40
An: Histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Bone Marrow Biopsy Fixation
Have you fixed bone marrow biopsies in formaling and if so for how long
and what decal solution is best
75 matches
Mail list logo
