Human or mouse? Are they trying to stain bone vasculature? If so I can
recommend superior markers.
Andrea
> On Dec 12, 2014, at 10:06 AM, Connolly, Brett M
> wrote:
>
> Hi all,
>
> A colleague is having problems staining CD31 on bone sections and is looking
> for recommendations for specifi
Hi all,
A colleague is having problems staining CD31 on bone sections and is looking
for recommendations for specific antibodies and antigen retrieval methods.
Can anyone help?
Thanks,
Brett
Brett M. Connolly, Ph.D.
Principle Scientist, Imaging Dept.
Merck & Co., Inc.
PO Box 4, WP-44K
West Poin
Hi,
Santacruz Goat sc-1506 works well on rabbit tissues. Good lucks.
On Tue, Oct 14, 2014 at 3:46 PM, Mandy Bell wrote:
> Hi,
>
> We are trying to run CD31 in rabbit tissue. We've tried a couple of
> different antibodies with no luck. Hoping someone can point me in the
> right direction- d
Hi,
We are trying to run CD31 in rabbit tissue. We've tried a couple of different
antibodies with no luck. Hoping someone can point me in the right direction-
does anyone have a CD31 they have had success with in rabbit tissue?
Thanks,
Mandy Bell, HTL(ASCP)
___
inal Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Thursday, February 14, 2013 12:27 PM
To: Jo-Ann Bader, Ms.; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] CD31 problewms
If you have not changed
J.
From: "Jo-Ann Bader, Ms."
To: "histonet@lists.utsouthwestern.edu"
Sent: Thursday, February 14, 2013 11:49 AM
Subject: [Histonet] CD31 problewms
We are having problems getting our CD31 to work well on our NBF fixed mouse
tissue. There is staining but it is very pale.
We are having problems getting our CD31 to work well on our NBF fixed mouse
tissue. There is staining but it is very pale.
The CD31 comes from Bio-care and we are are using the IntelliPATH stainer.
We have tried:
- Trypsine 37C 15 min / Trypsine RT 30 min
- Trypsine (1:2 and 1:3)
- With Decloak
Does anyone know if this clone WM59 crosses to mouse CD31?
Andrea
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r 21, 2012 11:20 AM
Subject: RE: [Histonet] CD31 for FFPE IHC on Mouse
Rat anti mouse cd31 from Dionova is the way to go it is better than all the
others.
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting, LLC
40864 Arkansas Ave
Bennett, CO 80102
Phone: 303-644-4538
Fax: 720-859-4110
pru...@i
, 2012 11:20 AM
To: 'Mark Elliott'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] CD31 for FFPE IHC on Mouse
Rat anti mouse cd31 from Dionova is the way to go it is better than all the
others.
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting, LLC
40864 Arkansas Ave
Bennett, CO 8
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Elliott
Sent: Friday, October 19, 2012 3:45 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CD31 for FFPE IHC on Mouse
Amy
Can you please share the responses you got with the rest of us
Thanks
Mark
Message
Amy
Can you please share the responses you got with the rest of us
Thanks
Mark
Message: 8
Date: Thu, 18 Oct 2012 16:21:55 -0400
From: "Amy Porter"
Subject: RE: [Histonet] CD31 for FFPE Immunohistochemistry on Mouse
Model
To: "'Amy Porter'" ,"'Histon
From: Amy Porter
To: 'Histonet'
Sent: Thursday, October 18, 2012 12:35 PM
Subject: [Histonet] CD31 for FFPE Immunohistochemistry on Mouse Model
Anyone out there have CD31 working well on FFPE samples for Mouse Samples??
I know this might be a long shot, however I haven't look
18, 2012 3:36 PM
To: 'Histonet'
Subject: [Histonet] CD31 for FFPE Immunohistochemistry on Mouse Model
Anyone out there have CD31 working well on FFPE samples for Mouse Samples??
I know this might be a long shot, however I haven't looked for anything on
this in quite awhile.
Am
Anyone out there have CD31 working well on FFPE samples for Mouse Samples??
I know this might be a long shot, however I haven't looked for anything on
this in quite awhile.
Amy S. Porter, HT(ASCP) QIHC
Michigan State University
Investigative HistoPathology Laboratory
William S. Spielman, Ph.
SC had a goat anti mouse CD31 and the goat croaked about 6 years ago. I have
yet to see a better CD31 in FFPE mouse.
-Original Message-
From: Patsy Ruegg
To: helen.ilsley ; histonet
Sent: Mon, Sep 17, 2012 12:38 pm
Subject: RE: [Histonet] Cd31
Unfortunately we do not have an anti
@lists.utsouthwestern.edu
Subject: [Histonet] Cd31
Hi
Which endothelial marker would you use for rat tissue?
I am really interested to see what you all say.
Thanks in advance
Helen
Sent from my BlackBerryR wireless device
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Histonet
Hi
Which endothelial marker would you use for rat tissue?
I am really interested to see what you all say.
Thanks in advance
Helen
Sent from my BlackBerry® wireless device___
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...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lucie
Guernsey
Sent: Friday, December 16, 2011 7:09 PM
To: Colleen Forster
Cc: Histonet
Subject: Re: [Histonet] CD31 for rat tissue
Colleen,
Unfortunately, I can't help you out, but I'm also interested in this
questi
Colleen,
Unfortunately, I can't help you out, but I'm also interested in this
question. If you happen to get responses off-list, can you share which rat
antibodies are suggested by others? Also, would you mind sharing which
mouse antibody and dilution you found works well?
Thanks!
Lucie
Lucie Gu
Hello Histonetters,
I am looking for a CD31 that has been proven to work well in rat tissue
samples...have any of you out there done this with success? I have good
human and mouse antibodies not rat!
Thanks in advance!
Colleen Forster
Bionet Histology Research Laboratory
U of MN
612-626-1930
I realize that CD31 staining has been discussed extensively in the past
on histonet, but typically the conversation revolves around which
fixatives to use for paraffin sections or the inability to find a good
CD31 antibody for mouse tissue.
I am simply looking for a CD31 antibody that works well o
ubject: RE: SPAM-LOW: Re: [Histonet] Cd31 in pigs
Yea you can use F8 and SMA for vessel lining cells but those will not pick
up all the developing endothelial cells like cd31 or cd34.
There is a new rat anti mouse CD31 from Japan, I wonder it if works on pig,
I will try it and see, it works great
, April 09, 2010 10:24 AM
To: Jean-Martin Lapointe; histonet@lists.utsouthwestern.edu
Subject: SPAM-LOW: Re: [Histonet] Cd31 in pigs
Von Willebrand Factor (Factor VIII) is also what I use to stain pig
endothelium.
Jan Shivers
Senior Scientist
Pathology Teaching Program
Histology/IHC/EM Section Head
delete this message and any attachments immediately.)
- Original Message -
From: "Jean-Martin Lapointe"
To:
Sent: Friday, April 09, 2010 10:59 AM
Subject: [Histonet] Cd31 in pigs
Hi Joel,
we have tried a few antibodies for CD31 in FFPE tissues in pigs and have had
n
Hi Joel,
we have tried a few antibodies for CD31 in FFPE tissues in pigs and have had no
luck. We currently use an antibody to vWF to stain pig endothelium. If someone
has a working technique for CD31 in swine, I'd be happy to hear about it.
Jean-Martin
__
Jean-Ma
Does anyone have a procedure that WORKS for cd31 in pigs? I can't seem
to get it to work no matter what I do. Any help will be greatly
appreciated. Thanks.- joel
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Hi, we use Abcam mouse-anti CD31
also acetone fixed frozen sections.
pretty well.
2010-03-02
TF
发件人： Hernandez, Anna
发送时间： 2010-03-02 09:50:38
收件人： Annette Featherstone; histonet@lists.utsouthwestern.edu
抄送：
主题： RE: [Histonet] CD31 in rats
Hello Annette,
We use Purified Mouse
Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Annette
Featherstone
Sent: Monday, March 01, 2010 7:45 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CD31 in rats
Is anyone using CD31 antibody in rats, if so
Is anyone using CD31 antibody in rats, if so, what vendor and what protocol.
thanks
Annette Featherstone
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
tonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nicholas
David Evans
Sent: Thursday, November 12, 2009 5:20 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CD31/PECAM antibody
Hello,
Can anyone suggest a good antibody to recognize mouse CD31 (PECAM)? I have
been trying to use BD
hi...that one works...according to my collegue
BUT...dont use PFA fixation
u can do frozen section, or parrafin section with Zinc fixation
2009-11-13
TF
发件人： Nicholas David Evans
发送时间： 2009-11-13 09:24:09
收件人： histonet@lists.utsouthwestern.edu
抄送：
主题： [Histonet] CD31/PECAM antibody
Hello,
Can anyone suggest a good antibody to recognize mouse CD31 (PECAM)? I have
been trying to use BD Pharmingen MEC 13.3 with little success. I use
vector lab's goat anti-rat (BA-9400) but get loads of background on mouse
sections with this secondary. I also tried a mouse-on-mouse kit and sa
ingham to help save on costs. I have a room reserved at DoubleTree
from Friday - Wednesday, but if you already have a room reserved and are
looking for someone to share costs, I can do that too.
Send me an email at mp...@alltech.com if you are interested
Thanks a lot!
Marquisha Paul
Researc
. Email me if you want
details on preparing it yourself.
Good luck,
Jameel
Message: 11
Date: Thu, 24 Sep 2009 13:46:16 -0500
From: JEFFREY S HARDING
Subject: [Histonet] CD31 Mouse Liver Staining
To: histonet@lists.utsouthwestern.edu
Message-ID: <7050ac4f65505.4abb7...@wiscmail.wisc.edu>
C
Hello,
I've tried a dozen times to get good fluorescent CD31 staining (endothelial
marker) on frozen mouse liver sections.
I'm using the standard pharmogen Anti-CD31 primary with a secondary FITC
label, and get a very weak (though noticable) signal.
My question is regarding fix
2009-08-18
TF
发件人： sotiris lakis
发送时间： 2009-08-16 17:57:03
收件人： histonet
抄送：
主题： [Histonet] CD31(PECAM-1) by novocastra on FFPE rabbit tissue
Hi,
I have question about CD31(PECAM-1) by novocastra. Has anybody worked with it
on FFPE rabbit tissue? I know for instance that DAKO&#
Hi,
I have question about CD31(PECAM-1) by novocastra. Has anybody worked with it
on FFPE rabbit tissue? I know for instance that DAKO's works, but could find no
references for the one by novocastra. Moreover I am using a Bond protocol that
comes with an antimouse/antirabbit IgG 2nd Ab. I coul
information.
dusko
From: "Zerfas, Patricia (NIH/OD/ORS) [E]"
To: dusko trajkovic
Sent: Monday, August 10, 2009 12:07:29 PM
Subject: RE: [Histonet] CD31 working protocol
Dusko,
I am using mouse tissue.
Patricia Zerfas
National Institutes of Healt
ePark Drive
Cambridge, MA 02140
--- On Mon, 8/10/09, Zerfas, Patricia (NIH/OD/ORS) [E]
wrote:
From: Zerfas, Patricia (NIH/OD/ORS) [E]
Subject: [Histonet] CD31 working protocol
To: "'histonet@lists.utsouthwestern.edu'"
Date: Monday, August 10, 2009, 2:50 PM
Dear Listservers,
:50 PM
To
"'histonet@lists.utsouthwestern.edu'"
cc
Subject
[Histonet] CD31 working protocol
Dear Listservers,
I have tried MANY protocols and I am unable to obtain a
working protocol for the CD31 antibody.
I have tried both the CD31 rat monoclonal
- Original Message - From: "Zerfas, Patricia (NIH/OD/ORS) [E]"
To:
Sent: Monday, August 10, 2009 2:50 PM
Subject: [Histonet] CD31 working protocol
Dear Listservers,
I have tried MANY protocols and I am unable to obtain a
working protocol for the CD31 antibody.
-3320
Phone: (517) 884-5026
Fax: (517) 432-1368
Email: port...@msu.edu
Web: www.humanpathology.msu.edu
- Original Message -
From: "Zerfas, Patricia (NIH/OD/ORS) [E]"
To:
Sent: Monday, August 10, 2009 2:50 PM
Subject: [Histonet] CD31 working protocol
Dear Listservers,
Dear Listservers,
I have tried MANY protocols and I am unable to obtain a working
protocol for the CD31 antibody.
I have tried both the CD31 rat monoclonal and a CD31 rabbit
polyclonal.
Thanks in advance for your help.
Patricia Zerfas
National Institutes of Health
Buildi
00:16:34
收件人： ti...@foxmail.com; Colleen Forster
抄送： histonet; ΕΛΕΝΗ ΚΟΝΣΟΛΑΚΗ
主题： RE: [Histonet] CD31 on rat brain
For mouse (specifically) have used CD31 from BD catalog number 550274. Its a
rat ant-mouse (Clone MEC13.3).
can supply more info if need.
The problem I have found is finding
e: 11
Date: Sat, 7 Mar 2009 00:44:32 +0800
From: "TF"
Subject: Re: RE: [Histonet] CD31 on rat brain
[Histonet] staining brain vessels
>
> I was wondering if anybody might have an idea with the following
> problem we are experiencing: we want to stain for blood vessels in
16:34
收件人： ti...@foxmail.com; Colleen Forster
抄送： histonet; ΕΛΕΝΗ ΚΟΝΣΟΛΑΚΗ
主题： RE: [Histonet] CD31 on rat brain
For mouse (specifically) have used CD31 from BD catalog number 550274. Its a
rat ant-mouse (Clone MEC13.3).
can supply more info if need.
The problem I have found is finding
samples to begin with.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of TF
Sent: Friday, March 06, 2009 10:23 AM
To: Colleen Forster
Cc: histonet; ΕΛΕΝΗ ΚΟΝΣΟΛΑΚΗ
Subject: [Histonet] CD31 on rat brain
Just wonder anyone has a suggestion of antibody that works on rat brain? Frozen
section and parafin sections?
It would be great if this also works on mice.
2009-03-06
TF
发件人： Colleen Forster
发送时间： 2009-03-06 00:57:29
收件人： tifei
抄送： iskaliora; histonet; ΕΛΕΝΗ ΚΟΝΣΟΛΑΚΗ
主题： Re: [Histo
I have used the antibody and kit from Biocare for CD31 and got good results.
Simple to use.
nancy.lo...@med.va.gov
--- On Fri, 2/13/09, Jackie M O'Connor wrote:
From: Jackie M O'Connor
Subject: Re: [Histonet] CD31 on FFPE mouse tissue
To: kmerriam2...@yahoo.com
Cc: "Histon
Does it have to be CD31? I have a bunch of good protocols for mouse
vessels but each tissue requires a different antibody. CD31 in FFPE
is never reliable in my experience. Which tissue are you using?
Andrea
Kim Merriam Sent by:
histonet-boun...@lists.utsouthwestern.edu
02/13/2009 12:27
his, but I would contact Biocare.
Kim Merriam
Sent by: histonet-boun...@lists.utsouthwestern.edu
02/13/2009 12:27 PM
Please respond to
kmerriam2...@yahoo.com
To
Histonet
cc
Subject
[Histonet] CD31 on FFPE mouse tissue
What is everyone doing for CD31 on FFPE mouse tissue? We are
Behalf Of Jackie M
O'Connor
Sent: Friday, February 13, 2009 11:33 AM
To: kmerriam2...@yahoo.com
Cc: Histonet; histonet-boun...@lists.utsouthwestern.edu
Subject: Re: [Histonet] CD31 on FFPE mouse tissue
Biocare Medical is supposed to have a good CD31 for FFPE mouse. I don't
have proof of t
Biocare Medical is supposed to have a good CD31 for FFPE mouse. I don't
have proof of this, but I would contact Biocare.
Kim Merriam
Sent by: histonet-boun...@lists.utsouthwestern.edu
02/13/2009 12:27 PM
Please respond to
kmerriam2...@yahoo.com
To
Histonet
cc
Subject
[Histonet]
What is everyone doing for CD31 on FFPE mouse tissue? We are looking to do IF,
but a DAB protocol would be just as good.
Thanks,
Kim
Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA
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