Biolegend). It is commonly used for sample preparation for flow
> cytometry. Check the SDS to make sure it does not contain formaldehyde.
>
>
> -Original Message-
> From: Mac Donald, Jennifer via Histonet
> [mailto:histonet@lists.utsouthwestern.edu]
> Sent: Friday, 1
ommonly used for sample preparation for flow
> cytometry. Check the SDS to make sure it does not contain formaldehyde.
>
>
> -Original Message-
> From: Mac Donald, Jennifer via Histonet
> [mailto:histonet@lists.utsouthwestern.edu]
> Sent: Friday, 17 January 2020 4:53 A
List
Subject: Re: [Histonet] Cell block preparations
Acetic acid would work.
Get Outlook for iOS<https://aka.ms/o0ukef>
From: Charles Riley via Histonet
Sent: Thursday, January 16, 2020 8:55:21 AM
To: Histo List
Subject: [Histonet] Cell block prepar
Acetic acid would work.
Get Outlook for iOS<https://aka.ms/o0ukef>
From: Charles Riley via Histonet
Sent: Thursday, January 16, 2020 8:55:21 AM
To: Histo List
Subject: [Histonet] Cell block preparations
EXTERNAL SENDER- Exercise caution with requests,
What is the best way to remove excess blood from FNA sample collections
before spinning them down into cell blocks?
--
Charles Riley BS HT, HTL(ASCP)CM
Histopathology Coordinator/ Mohs
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
raud via Histonet histonet@lists.utsouthwestern.edu
> Sent: Monday, October 28, 2019 10:14 AM
> To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Cell block processing
>
> [External Email] This email originated from outside of the organizatio
Sent: Monday, October 28, 2019 10:14 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] Cell block processing
[External Email] This email originated from outside of the organization. Think
before you click: Don’t click on links, open attachments or respond to requests
We collect our FNAs in CytoLyt. We also use it to wash all our non-gyn fluids,
but then we fix the cell block "pellet" in formalin.
We have had no problems with immunos, and are able to lyse the RBCs to provide
a nice, clear specimen.
Hope this helps.
Terri L. Braud, HT(ASCP)
Anatomic
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
From: Charles Riley via Histonet
Sent: Friday, 25 October 2019 23:12
To: Histo List
Subject: [Histonet] Cell block processing
Does anyone have any tips
ober 25, 2019 12:57 PM
To: Joe W. Walker, Jr.
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Cell block processing
[External Email] This email originated from outside of the organization. Think
before you click: Don’t click on links, open attachments or respond to requ
utilize for this
process. The cell blocks cut great, look great, and you can perform IHC an
molecular testing if needed.
Joe Walker
From: Charles Riley
Sent: Friday, October 25, 2019 12:57 PM
To: Joe W. Walker, Jr.
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Cell block
t; joewal...@rrmc.org, www.rrmc.org
>
> -Original Message-
> From: Charles Riley via Histonet
> Sent: Friday, October 25, 2019 8:13 AM
> To: Histo List
> Subject: [Histonet] Cell block processing
>
> [External Email] This email originated from outside of the organi
: [Histonet] Cell block processing
[External Email] This email originated from outside of the organization. Think
before you click: Don’t click on links, open attachments or respond to requests
for sensitive information if the email looks suspicious or you don’t recognize
the sender.
Does
Does anyone have any tips or suggestions on how to better process extremely
bloody FNA specimens?Is there anyway to clear out some or all of the
blood without destroying the other tissues?
--
Charles Riley BS HT, HTL(ASCP)CM
Histopathology Coordinator/ Mohs
Is there any way to save cell blocks that have been rehydrated to much?
Tech left them on their ice bath too long and they have become really soft.
--
Charles Riley BS HT, HTL(ASCP)CM
Histopathology Coordinator/ Mohs
___
Histonet mailing list
We use a 50:50 ratio, 1 ml of each to cell button.
>
> On Apr 25, 2018 at 1:11 PM, (mailto:histonet@lists.utsouthwestern.edu)> wrote:
>
>
>
> Does anyone have the recipe for making cell blocks with Thrombin and Plasma?
> We made them at my first Histo job, oh
Does anyone have the recipe for making cell blocks with Thrombin and Plasma?
We made them at my first Histo job, oh say over 20 years ago but I do not
remember the ratio.
Thanks bunches,
Lisa
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
Hello all,
Our pathologists are worried about our cell yield with our cell blocks.
Can anyone share their protocols and what you use to make the cell blocks.
I do not know what our protocol is as they are made by the second shift
cytoprep team and I work the early shift
--
Charles Riley
Does anyone have a cell block protocol they use for Ultra sound Fine needle
aspirates? We have been experiencing some issues with the tissue falling
off slides and washing off immunostains. Any help would be greatly
appreciated
--
Charles Riley HT(ASCP)CM
Histopathology Coordinator/ Mohs
We use HistoGel instead of Cellblock with 100% capture of the specimen
(validated) and no peeling or sliding off of slides.
Hope that helps.
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com
On 12/17/15, 7:16
Hi everyone,
Someone in the lab wishes to look at cultured cells in paraffin embedded cell
blocks. These cells grow mostly in non-adherent clumps or cell bodies, which
need to be preserved. Sections will be stained by IHC.
I've been looking into the matter, and it seems there is not really a
We have something of a mystery here and I am hoping someone can help. My
cytopath docs are saying that our B72.3 (TAG-72) antibody is not working well
in cell blocks created from fluids (plural), etc. and that this is a fairly
recent development (over the past 5-6 weeks or so).The
, September 05, 2013 12:45 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cell Block Preparation
I am getting complaints in regard to insufficient cell blocks. We currently
spin, pour off the supernatant, retrieve the sediment and process in lens paper.
Does anyone have a more current
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Specian
Sent: Thursday, September 05, 2013 12:45 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cell Block Preparation
I am getting complaints in regard to insufficient cell blocks. We currently
spin, pour off
'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Cell Block Preparation
This is how we do it now. In the old days, we used agar and to my mind, it is
still the best way when you have scant material.
- Spin in a conical tube and pour off
- Melt an agar slant (we get TSA slant from micro
Of
Rathborne, Toni
Sent: Friday, September 06, 2013 7:18 AM
To: 'Tom McNemar'; 'Ann Specian'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Cell Block Preparation
I wonder if this method could be used with the product Histogel. Has
anyone tried it?
-Original Message-
From: histonet-boun
This is a pretty good method for scant specimens. I have even used it for CSFs
that have malignancy with success.
http://www.jove.com/video/1316/cell-block-preparation-from-cytology-specimen-with-predominance
CONFIDENTIALITY NOTICE:
This e-mail message, including all attachments, is for the
12:45 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cell Block Preparation
I am getting complaints in regard to insufficient cell blocks. We
currently spin, pour off the supernatant, retrieve the sediment and
process in lens paper.
Does anyone have a more current technique which
, September 05, 2013 12:45 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cell Block Preparation
I am getting complaints in regard to insufficient cell blocks. We
currently spin, pour off the supernatant, retrieve the sediment and
process in lens paper.
Does anyone have a more
[mailto:thisis...@aol.com]
Sent: Thursday, September 05, 2013 12:45 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cell Block Preparation
I am getting complaints in regard to insufficient cell blocks. We
currently spin, pour off the supernatant, retrieve the sediment and
process in lens
I am getting complaints in regard to insufficient cell blocks. We currently
spin, pour off the supernatant, retrieve the sediment and process in lens paper.
Does anyone have a more current technique which renders better cellularity?
Also, do you know which renders a better cell block: a
Hey guys-- looking to collect as many different cell block prep procedures as I
can find. Want the best outcome for my lab!
What do you have/ what have you do e that really works?
Cheryl
Eric--I cleaned off the thread! You should be so proud !!!
Sent from my iPhone
Hello everyone!
In our lab, we've been trying out the Shandon CB kit and we've been having
a few problems. We use non-buffered 10% formalin as a fixative (overnight),
follow the manual's instructions in regards to ratio of reagents/size of
pellet and follow a regular 10h histological processing
A couple of things i did when i had this same issue at one time was If the fna
had teeny core biopsies we would separate into two blocks. This allowed for one
block to get all the usual in house antibodies and the other could be sent out
for special testing. They both got HE s. another thing is
Happy Friday Histonetters,
We have a pathologist that will be ordering all the latest test such as MMR -
ALK Mutation and all of the other good ones that oncologist will be relying on
to treat their patients. He wants to order these test on cell block material
that he gets from FNA's. As is we
Hello all,
I'm interested to see what staining protocol folks are using for HE staining
of cell blocks. Lately we've been getting varying light and dark staining on
cell blocks only. Currently they are run on our standard HE protocol that we
use for all tissues. Does anyone use a separate
, 8/10/11, Dessoye, Michael J mjdess...@wvhcs.org wrote:
From: Dessoye, Michael J mjdess...@wvhcs.org
Subject: [Histonet] Cell block HE staining
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, August 10, 2011, 10:35 AM
Hello all,
I'm interested to see what staining protocol folks
Hello Histo folks
Would you all mind sharing with me your procedures for making cell
blocks? I would like to compare different procedures with what I am
doing.
Thanks so much
Kimberly
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
If one is in a hospital setting, I just go into Micro and get a TSA
slant. Loosen the top and pop it in the microwave for 5-10 seconds. Ta-daa!
Cell block!
Sincerely,
Jay
A. Lundgren M.S., HTL (ASCP)
___
Histonet mailing list
I was wondering if anyone has a technique or method for making agar for cell
blocks. I was also looking for manufactured agar and where I can get it from.
Thanks in advance!
Kind regards,
Michele Carr HTL ASCP
___
Histonet mailing list
: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michele Carr
Sent: Friday, March 18, 2011 11:04 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cell block agar
I was wondering if anyone has a technique or method for making agar
@lists.utsouthwestern.edu
Subject: [Histonet] cell block fixation
We recently switched vendors for our formalin and while we have not
experienced any difference with our surgical specimens, our cell blocks
from body fluids have been giving us a great deal of trouble. The
button that we get never seems
...@lists.utsouthwestern.edu] On Behalf Of Feher,
Stephen
Sent: Monday, February 14, 2011 9:31 AM
To: Hutton, Allison; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] cell block fixation
Try a 50/50 mix of formalin and 95% alcohol. Have your prep techs add
about 5 mL of this mixture
We recently switched vendors for our formalin and while we have not experienced
any difference with our surgical specimens, our cell blocks from body fluids
have been giving us a great deal of trouble. The button that we get never
seems to harden, leaving it sort of gelatinous, even if left to
44 matches
Mail list logo
