Thanks all, looks like the consensus is that water into the clearing agent
causes it and I should change out alcohol more frequently.
Allan
On Wed, Oct 31, 2018 at 5:46 PM Allan Wang wrote:
> Hi histonet,
>
> For coverslipping Ventana IHC slides I have dish soap, rinse, alcohol 80%,
> 100%,
Hi histonet,
For coverslipping Ventana IHC slides I have dish soap, rinse, alcohol 80%,
100%, 100%, 100%, followed by three Clear-Rite 3 instead of xylene. In the
past I didn't have this issue, but now I frequently get a lot of residue on
the slides after they enter the Clear-Rite.
If I
Richard W. Cartun, MS, PhD - Director, Histology & The Martin M. Berman, MD
Immunopathology & Morphologic Proteomics Laboratory - Director, Biospecimen
Collection Programs - Assistant Director, Anatomic Pathology, Hartford
Hospital, Hartford, CT asks
>>Anyone using ClearRite (xylene replacement)
Greetings!
We have been using Clear-Rite 3 here at our lab, and we are happy with
the product. Our supplier says it will be on back-order for some time
now. Our crack supply folks are looking for another source. I'm taking
another route to find out what products out there are comparable.
Are
] Clear-Rite 3
To: Histonet@lists.utsouthwestern.edu
Date: Wednesday, August 5, 2009, 9:11 AM
Greetings!
We have been using Clear-Rite 3 here at our lab, and we are happy with
the product. Our supplier says it will be on back-order for some time
now. Our crack supply folks are looking for another
, Bill billodonn...@catholichealth.net
To: Histonet@lists.utsouthwestern.edu
Sent: Wednesday, August 05, 2009 9:11 AM
Subject: [Histonet] Clear-Rite 3
Greetings!
We have been using Clear-Rite 3 here at our lab, and we are happy with
the product. Our supplier says it will be on back-order for some
; BillO'Donnell
Subject: Re: [Histonet] Clear-Rite 3
Bill:
Xylene substitutes are not all pretty much the same. There are d-Limonene
derivatives (something to stay absolutely away from) and alkane derivatives
with many known and unknown constituents.
Since you are about to change and not have any
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell,
Bill
Sent: Wednesday, August 05, 2009 9:12 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Clear-Rite 3
Greetings!
We have been using Clear-Rite 3 here at our lab, and we are happy with
the product. Our
: [Histonet] Clear-Rite 3
To: Histonet@lists.utsouthwestern.edu, BillO'Donnell
billodonn...@catholichealth.net
Date: Wednesday, August 5, 2009, 2:01 PM
Bill:
Xylene substitutes are not all pretty much the same. There are d-Limonene
derivatives (something to stay absolutely away from) and alkane derivatives
Sicurello
Sent: Wednesday, August 05, 2009 12:49 PM
To: Rene J Buesa; HistoNet
Subject: Re: [Histonet] Clear-Rite 3
Hi Rene,
Why do you not like the d-limonene solvents?
I work with them and the results seem fine. I have to mention that I use an
AutoTechnicon Duo and cannot place it in a fume hood
: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Va Paula
Sicurello
Sent: Wednesday, August 05, 2009 12:49 PM
To: Rene J Buesa; HistoNet
Subject: Re: [Histonet] Clear-Rite 3
Hi Rene,
Why do you not like the d-limonene solvents?
I work
My answer is in the attached article I just published. Please read the
d-Limonene discussion seciton.
René J.
--- On Wed, 8/5/09, Sherwood, Margaret msherw...@partners.org wrote:
From: Sherwood, Margaret msherw...@partners.org
Subject: RE: [Histonet] Clear-Rite 3
To: Va Paula Sicurello vapat
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